CN102988533A - Application of coffee residue extract in preparing medicaments - Google Patents
Application of coffee residue extract in preparing medicaments Download PDFInfo
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- CN102988533A CN102988533A CN2011102831947A CN201110283194A CN102988533A CN 102988533 A CN102988533 A CN 102988533A CN 2011102831947 A CN2011102831947 A CN 2011102831947A CN 201110283194 A CN201110283194 A CN 201110283194A CN 102988533 A CN102988533 A CN 102988533A
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Abstract
The invention provides application of coffee residue extract in preparing medicaments. The invention relates to an extract obtained from coffee residues and application thereof in preparing a medicament, and belongs to the field of medicine. Thin-layer identification indicates that the extract mainly comprises triterpene compounds, and has an inhibition effect to increased permeability of mouse peritoneal capillary, can be used for reducing the dimethylbenzene induced mouse ear swelling rate, and has certain anti-inflammation effect. According to the application, residues are utilized, and the coffee residue extract is applied to medicament preparation.
Description
Technical field:
The present invention relates to from spent coffee residues, extract and separate a kind of extract and application aspect the preparation medicine thereof that contains triterpenes components, belong to pharmaceutical field.
Background technology:
Instant coffee factory has produced a large amount of spent coffee residues when producing instant coffee, these waste residues are mostly by garbage disposal.According to United States Department of Agriculture's statistics, there is about 7,000,000,000 kilograms coffee output in the whole world every year, and these coffees are after edible or extraction, and the spent coffee residues amount of generation is extremely considerable.Along with environmental protection and low-carbon (LC) become the world idea gradually, the twice laid of coffee grounds research has obtained larger progress, utilizes spent coffee residues refinement biodiesel such as the scientist of Japan and the U.S.; Other has the bibliographical information Action of Extracts from Coffee Residue to have antioxidation and can extract glucide from spent coffee residues, yet the preparation extract is used for antiinflammatory from spent coffee residues, has not yet to see report.Studies show that, many triterpenoid compound have obvious antiinflammatory action, and just contain a large amount of triterpene substances in the spent coffee residues.
Purpose of the present invention will provide a kind of spent coffee residues extract exactly, and it contains triterpene substance, has significant antiinflammatory action, can be used for the application that medicine prepares the aspect.
Summary of the invention:
Spent coffee residues extract of the present invention is selected optimum extracting method through testing sieve, shows that through thin layer chromatography its triterpenes components content is higher.After obtaining this extract, through Hainan Medical College Hainan Province torrid zone medicinal plants study and developing focus laboratory it acute toxicity test and antiinflammatory pharmacodynamics test have been carried out.
One, the preparation of spent coffee residues extract:
The spent coffee residues extract forms in order to the below legal system is standby: get the spent coffee residues of certain mass, add a certain amount of alcohol reflux, collect extracting solution and be condensed into the extractum shape, obtain Action of Extracts from Coffee Residue.
This spent coffee residues extract differentiates that through chromogenic reaction inspection knowledge and thin layer demonstration contains triterpenes components.
Chromogenic reaction: get extract on a small quantity in vitro, with the acetic anhydride dissolving, carry out acetic anhydride-concentrated sulphuric acid chromogenic reaction, the result: solution finally takes on a red color.
Thin layer chromatography is differentiated: developing solvent: petroleum ether-acetone (4: 1), and the result: immaculate under the visible light, spray the colour developing of 10% ethanol solution of sulfuric acid, observed four speckles under the visible light and occurred.
The thin layer chromatography identification result shows and contains triterpenes components in the extract.
The preparation method of spent coffee residues extract of the present invention is preferably: get the spent coffee residues of certain mass, add the alcohol reflux 1～3 time of 1～20 times of amount, collect merge extractive liquid, and also be condensed into the extractum shape, obtain Action of Extracts from Coffee Residue.
Concentration of alcohol is preferred 50%～95% in the preparation spent coffee residues extract method, preferably uses 95% ethanol; Preferred 5～10 times of amounts of ethanol consumption are preferably 8 times of amounts; Extraction time is preferably 3 hours; Extraction time is preferably 2 times.
Two, acute toxicity test
1. test material:
SPF level Kunming mouse, male and female half and half, body weight 18～22g; The mouse stomach device; Tween 80
2. test method and result:
Get the spent coffee residues extract, take tween 80 as cosolvent, add water and be made into Cmax suspension (0.43gml
-1).Trial test: get Kunming mouse, male and female half and half are divided into 3 groups at random, 4 every group, after water 12h is can't help in fasting, press respectively 8.60gkg in one day
-1, 12.70gkg
-1, 17.20gkg
-1Gavage gives spent coffee residues extract (each gavage volume 0.2ml/10g, maximum administrations are 2 times in 1 day), observes mice survival condition, Continuous Observation 7 days after the administration.The results are shown in Table 1.
The preliminary experiment result can't find out its 100% fatal dose, and formal test wants to find out its maximum tolerated dose.
Formal test: get Kunming mouse, male and female half and half are divided into 3 groups at random, and 20 every group, after water 12h was can't help in fasting, administration group respectively gavage gave spent coffee residues extract 8.60gkg
-1, 10.75gkg
-1, the matched group gavage gives 1% tween 80 aqueous solution.The observation autonomic activities changes, Continuous Observation 14 days.The results are shown in following table 2.
Table 1 acute toxicity trial test result
Table 2 acute toxicity formal test result
Autonomic activities reduces after the mice administration, and idol has manic uneasiness, and administration is after 2 days, and it is normal that all mice autonomic activitieses recover.
Above result of the test shows, spent coffee residues extract toxicity is lower, and the maximum tolerated dose of its mice is 8.60gkg
-1
Three, antiinflammatory pharmacodynamics test
1. suppress the test of mice caused by dimethylbenzene xylene ear swelling.Get the spent coffee residues extract, take tween 80 as cosolvent, add water and be made into the variable concentrations suspension,
Get 50 of 18～22g Kunming kind white mouse, male and female half and half, are divided into totally 5 groups of the high, medium and low dosage group of spent coffee residues extract, aspirin group, blank groups at random by 10 every group.Every group respectively gavage give relative medicine, once a day, successive administration 7 days.Behind the 7th day administration 1h, dimethylbenzene 20 μ L evenly are applied to the left ear of white mice two sides, auris dextra in contrast, to scorching 30min, put to death mice, cut two ears along the auricle line, be that the 8mm card punch is got left and right sides auricle with diameter, use immediately scales/electronic balance weighing, take the difference of two ear weight as swelling.Adopt t method of inspection relatively each administration group and the blank group of matched group difference.The results are shown in Table 3.
Swelling rate=(left ear quality-auris dextra quality)/auris dextra quality * 100%.
2. suppress acetic acid and cause mouse peritoneal capillary permeability increase test
Get 50 of 18～22g Kunming mouses, male and female half and half, are divided into totally 5 groups of the high, medium and low dosage group of spent coffee residues extract, aspirin group, blank groups at random by 10 every group.Every group respectively gavage give relative medicine, once a day, successive administration 7 days.Behind the 7th day administration 1h, every mouse tail vein injection 0.5% azovan blue normal saline solution 0.1ml/10g (body weight), lumbar injection 0.6% glacial acetic acid 0.2ml/ only immediately, sacrificed by decapitation mice behind the 15min, cut off mouse web portion, with 6ml normal saline washing abdominal cavity, draw approximately 5ml of abdominal cavity eluate, 3000rmin with suction pipe
-1Centrifugal 10min gets ultraviolet spectrophotometer mensuration 590nm place's absorbance (Abs) on the supernatant.Adopt t method of inspection relatively each administration group and the blank group of matched group difference.The results are shown in Table 4.
Table 3 spent coffee residues extract xylol causes the effect of mice ear
Annotate: compare with the blank group: * p＜0.05
Table 4 spent coffee residues extract Dichlorodiphenyl Acetate causes the effect that mouse peritoneal capillary permeability increases
Annotate: carefully compare with blank: * p＜0.05
Above result shows: the spent coffee residues extract has preferably antiphlogistic effects, and along with the increase antiinflammatory action of dosage is more remarkable.
Four, the application of spent coffee residues extract in the preparation medicine
But spent coffee residues extract compatibility other drug adds relevant auxiliary materials, makes the clinically medicine of acceptable various dosage forms, wherein preferably makes tablet, capsule, granule.
The specific embodiment:
Embodiment 1:
Get spent coffee residues 400g, add 50% ethanol 8000ml, heating and refluxing extraction 3 times, each 1h filters and collects filtrate, distinguishes the flavor of into the thick paste shape with Rotary Evaporators Recycled ethanol to extracting solution without alcohol.
Embodiment 2:
Get spent coffee residues 400g, add 80% ethanol 4000ml, heating and refluxing extraction 1h filters and collects filtrate, and Recycled ethanol to extracting solution is distinguished the flavor of without alcohol, is concentrated into the thick paste shape.
Embodiment 3:
Get spent coffee residues 400g, add 95% ethanol 3200ml, heating and refluxing extraction twice is extracted 2h for the first time, extracts 1h for the second time, filters and collects filtrate, and Recycled ethanol to extracting solution is distinguished the flavor of without alcohol, is concentrated into the thick paste shape.
Embodiment 4:
Get spent coffee residues 400g, add 95% ethanol 800ml, heating and refluxing extraction 0.5h filters and collects filtrate, and Recycled ethanol to extracting solution is distinguished the flavor of without alcohol, is concentrated into the thick paste shape.
Embodiment 5:
Get spent coffee residues 400g, add 95% ethanol 2000ml, heating and refluxing extraction 2 times, 1h for the first time, 0.5h filters and collects filtrate for the second time, and Recycled ethanol to extracting solution is distinguished the flavor of without alcohol, is concentrated into the thick paste shape.
Embodiment 6:
Get spent coffee residues extract among the embodiment 3, add disintegrating agent, binding agent, lubricant and filler and make tablet.
Embodiment 7:
Get spent coffee residues extract among the embodiment 3, add binding agent, lubricant, the agent of filler granulation.
Embodiment 8:
Get spent coffee residues extract among the embodiment 3, adding binding agent, lubricant, filler are made capsule.
Claims (6)
1. a spent coffee residues extract is characterized in that: contain triterpenes components, confirm to have antiinflammatory action through zoopery.
2. extract as described in claim 1, its preparation method is: get the spent coffee residues of certain mass, add the alcohol reflux 1～3 time of 1～20 times of amount, collect merge extractive liquid, and also be condensed into the extractum shape.
3. extract as described in claim 2, concentration of alcohol is 50%～95% in its preparation method, amount of alcohol is 5～10 times of amounts.
4. extract as described in claim 2, concentration of alcohol is 95% in its preparation method, and amount of alcohol is 8 times of amounts, and extraction time is 2 hours, and extraction time is 2 times.
5. with spent coffee residues extract claimed in claim 1, the compatibility other drug also adds relevant auxiliary materials, makes the clinically medicine of acceptable various dosage forms.
6. the medicine that contains the spent coffee residues extract as claimed in claim 5, its dosage form is tablet, granule, capsule.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102831947A CN102988533A (en) | 2011-09-14 | 2011-09-14 | Application of coffee residue extract in preparing medicaments |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102831947A CN102988533A (en) | 2011-09-14 | 2011-09-14 | Application of coffee residue extract in preparing medicaments |
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| CN102988533A true CN102988533A (en) | 2013-03-27 |
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| CN2011102831947A Pending CN102988533A (en) | 2011-09-14 | 2011-09-14 | Application of coffee residue extract in preparing medicaments |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110167400A (en) * | 2016-12-29 | 2019-08-23 | 皇家飞利浦有限公司 | Coffee is brewed |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN85107887A (en) * | 1984-01-18 | 1987-05-20 | 通用食品公司 | Finish coffee hydrolysis and oil extraction simultaneously |
-
2011
- 2011-09-14 CN CN2011102831947A patent/CN102988533A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN85107887A (en) * | 1984-01-18 | 1987-05-20 | 通用食品公司 | Finish coffee hydrolysis and oil extraction simultaneously |
Non-Patent Citations (1)
| Title |
|---|
| 潘文浩等: "咖啡渣提取物抗氧化性及其协同效应的研究", 《食品工业科技》, vol. 29, no. 11, 31 December 2008 (2008-12-31) * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110167400A (en) * | 2016-12-29 | 2019-08-23 | 皇家飞利浦有限公司 | Coffee is brewed |
| US11672375B2 (en) | 2016-12-29 | 2023-06-13 | Koninklijke Philips N.V. | Coffee brewing |
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Application publication date: 20130327 |