CN102100758A

CN102100758A - Qingpeng gel for relieving pain and subdhing swelling and preparation method thereof

Info

Publication number: CN102100758A
Application number: CN2010106109851A
Authority: CN
Inventors: 刘海龙; 张国霞; 陈丽娟; 张樱山; 卢伍党
Original assignee: Tibet Cheezheng Tibetan Medicine Co Ltd
Current assignee: Tibet Cheezheng Tibetan Medicine Co Ltd
Priority date: 2010-12-29
Filing date: 2010-12-29
Publication date: 2011-06-22
Anticipated expiration: 2030-12-29
Also published as: CN102100758B

Abstract

The invention provides gel for subdhing swelling and relieving pain, which comprises an active ingredient and a gel substrate, wherein the active ingredient is prepared from the following raw material medicaments in part by weight: 80 to 120 parts of oxytropis, 30 to 70 parts of sub-rhubarb, 50 to 100 parts of tiebangchui aconite tuber, 80 to 120 parts of core removal medicine terminalia fruit, 80 to 120 parts of terminalia bellerica, 80 to 120 parts of emblic leafflower fruit, 20 to 50 parts of benzoin, 130 to 170 parts of Chinese tinospora stem and 10 to 40 parts of musk; and the substrate comprises the following auxiliary materials in part by weight: 40 to 1,600 parts of gel framework material. The gel has the advantages of no foreign body sensation, high adhesiveness at positions of pathological changes, no irritation to skin mucous membranes, no pollution of clothes and the like.

Description

A kind of blue or green roc gel that is used for alleviating pain and detumescence and preparation method thereof

Technical field

The present invention relates to a kind of pharmaceutical composition and preparation method thereof, particularly a kind of gel and preparation method thereof.

Background technology

To be the doctor among the people of Tibetan carry according to " Tibetan medicine doctor determine addendum release difficulty ", the Four-Volume Medical Code two secretaries blue or green roc ointment, and in conjunction with the classical Tibetan medicine and pharmacology proved recipe that practical experience is formed, prescription records in first 322 pages of " health drug standard promulgated by the ministries or commissions of the Central Government " 95 years version Tibetan medicines.The prescription of blue or green roc ointment is:

Herba Oxytropis falcatae 100g, sub-rhubarb extract 50g, Radix aconiti szechenyiani 75g, Fructus Chebulae 100g, Fructus Terminaliae Billericae 100g, Fructus Phyllanthi 100g, Benzoinum 35g, Herba Lycopodii 150g, Moschus 25g.

Method for making: above nine flavors, except that other porphyrize powder of Moschus, all the other are ground into fine powder altogether, sieve, and add the Moschus fine powder, and mixing is with 8 years old urine of baby boys, Adeps Sus domestica or old butter furnishing ointment, promptly.

Function with cure mainly: alleviating pain and detumescence.Be used for the fever that swells and ache that gout, arthralgia chiefly caused by damp pathogen, " lower limb vasculitis ", " yellow fluid " disease etc. cause, herpes, pestilence pestilence fever etc.

The doctor among the people of Tibetan is according to the Four-Volume Medical Code, " brilliant pearl book on Chinese herbal medicine " record, in conjunction with long-term practical experience, with Herba Oxytropis falcatae, sub-rhubarb extract, Radix aconiti szechenyiani, Fructus Chebulae, Fructus Terminaliae Billericae, Fructus Phyllanthi, Benzoinum, Herba Lycopodii, Moschus are the blue or green roc ointment of the Tibetan medicine of raw material production, are subjected to the trust of extensive patients with notable therapeutic effect, but the blue or green roc ointment of traditional Tibetan medicine preparation technology is modulated into ointment after all crude drug fine powders are mixed, its technology is numerous and diverse, and batch production cycle is long, the production technology instability; Raw material and adjuvant source are unstable, are unfavorable for suitability for industrialized production; Part drug osmotic ability, bioavailability is low, can not guarantee curative effect fully, should not bring into play the curative effect of medicine; Product should not be deposited in addition, carries inconvenience; Because each side has greatly influenced the curative effect and the popularization of product.

At present, be not that basic square tube is crossed dosage changing form and prepared the document of gel and the report of product not still about prescription with the blue or green roc ointment of traditional Tibetan medicine.

Summary of the invention

At the existing in prior technology deficiency, the object of the present invention is to provide a kind of prescription with the blue or green roc ointment of traditional Tibetan medicine is that basic square tube is crossed the blue or green roc gel that is used for alleviating pain and detumescence that dosage changing form prepares, and another object of the present invention also is to provide the preparation method of this green grass or young crops roc gel.

According to purpose of the present invention, the invention provides a kind of blue or green roc gel that is used for reducing swelling and alleviating pain, it comprises active component and gel-type vehicle, wherein,

Described active component is made by following raw materials in weight portion medicine:

Herba Oxytropis Kansuensis 80-120 weight portion, sub-rhubarb extract 30-70 weight portion, Radix aconiti szechenyiani 50-100 weight portion, Fructus Chebulae (pitted) 80-120 weight portion, Fructus Terminaliae Billericae 80-120 weight portion, Fructus Phyllanthi 80-120 weight portion, Benzoinum 20-50 weight portion, Herba Lycopodii 130-170 weight portion, Moschus 10-40 weight portion; And

Described gel-type vehicle comprises the adjuvant of following proportioning:

Gel skeleton material 40～1600 weight portions.

Described gel-type vehicle also can further comprise the transdermal enhancer of 0.01-450 weight portion.

Described gel-type vehicle also can further comprise the wetting agent of 0.01-550 weight portion.

Described gel-type vehicle also can further comprise the pH regulator agent of 0.01-600 weight portion.

Described gel-type vehicle also can further comprise the stabilizing agent of 0.01-350 weight portion.

Described gel-type vehicle also can further comprise the surfactant of 0.01-250 weight portion.

Described gel-type vehicle also can further comprise the antibacterial of 0.01-30 weight portion.

Wherein, described gel skeleton material can be and be selected from carbomer, sodium alginate, poloxamer, polyox-yethylene-polyoxypropylene block copolymer, Polyethylene Glycol based block copolymer, poly--2-ethoxy ethylene ether-poly-hydroxyethyl vinyl Ether block copolymer, poly-N N-isopropylacrylamide, polyacrylic acid, sodium polyacrylate, gelatin, methylcellulose, ethyl cellulose, sodium carboxymethyl cellulose, chitosan, goes one or more in the acetyl-removed gellan gum, but is not limited thereto.

Wherein, described transdermal enhancer can be and is selected from Borneolum Syntheticum, Camphora, Mentholum, menthol, methyl salicylate, laurocapram, Decylmethyl Sulphoxide and the plant Oleum sesami one or more, but is not limited thereto.

Wherein, described wetting agent can be and is selected from glycerol, Polyethylene Glycol, sorbitol, propylene glycol, paraffin oil, mineral oil, paraffin, squalane, silicone oil aliphatic alcohol and the wax fat one or more.

Wherein, described pH regulator agent can be and is selected from ammonia, triethanolamine, sodium hydroxide, aluminium hydroxide, sodium bicarbonate, sodium hydrogen phosphate and the sodium citrate one or more, but is not limited thereto.

Wherein, described stabilizing agent can be and is selected from sodium pyrosulfite, sodium sulfite, sodium sulfite, sodium thiosulfate, ascorbic acid, disodiumedetate, ethylenediaminetetraacetic acid, citric acid and the tartaric acid one or more, but is not limited thereto.

Wherein, described surfactant can be and is selected from Polysorbate and the sodium lauryl sulphate one or both, but is not limited thereto.

Wherein, described antibacterial can be and is selected from parabens, phenethanol, sodium benzoate and the sorbic acid one or more, but is not limited thereto.

Wherein, in described active component:

The 10%-100% weight of the mixed material medicine of Herba Oxytropis Kansuensis, sub-rhubarb extract, Radix aconiti szechenyiani, Fructus Chebulae (pitted), Fructus Terminaliae Billericae, Fructus Phyllanthi, Benzoinum and Herba Lycopodii is added water or 30-90% ethanol is made the concentrated solution a that relative density is 1.0-1.5, residue mixed material medicine is made the medicated powder that particle diameter is 0.1-150 μ m; And

Moschus is made the fine powder that particle diameter is 1-180 μ m.

Gel of the present invention can adopt this area existing conventional technology to prepare, and perhaps also can prepare according to method provided by the invention.The invention provides a kind of preparation method of described gel, it can comprise the steps:

1) Herba Oxytropis Kansuensis, sub-rhubarb extract, Radix aconiti szechenyiani, Fructus Chebulae (pitted), Fructus Terminaliae Billericae, Fructus Phyllanthi, Benzoinum, Herba Lycopodii are mixed;

2) take by weighing the 10%-100% of above-mentioned mixed material medicine gross weight, adopt water or 30-90% ethanol extracts, concentrate, obtaining relative density is the concentrated solution a of 1.0-1.5, and will remain mixed material medicated powder and be broken into the medicated powder that particle diameter is 0.1-150 μ m;

3) Moschus is ground to form the fine powder that particle diameter is 1-180 μ m;

4) gel skeleton material adding distil water is soaked, make its abundant swelling, add one or more adjuvants that are selected from pH regulator agent, stabilizing agent, transdermal enhancer, wetting agent, surfactant and the antibacterial selectively, stir, make gel-type vehicle;

5) with step 2) gel-type vehicle that obtains of the fine powder that obtains of the concentrated solution a that obtains and medicated powder, step 3) and step 4) mixes, and it is an amount of to add water, stirs, and is described gel.

Wherein, in step 2) in, can adopt water boiling and extraction method, ethanol reflux extraction, ethanol percolate extraction method or alcohol dipping extraction method to extract.

The another kind of preparation method of gel of the present invention can may further comprise the steps:

1) Herba Oxytropis Kansuensis, sub-rhubarb extract, Radix aconiti szechenyiani, Fructus Chebulae (pitted), Fructus Terminaliae Billericae, Fructus Phyllanthi, Benzoinum, Herba Lycopodii are mixed, be ground into the medicated powder that particle diameter is 0.1-150 μ m;

2) Moschus is ground to form the fine powder that particle diameter is 1-180 μ m; And

3) gel skeleton material adding distil water is soaked, make its abundant swelling, add one or more adjuvants that are selected from pH regulator agent, stabilizing agent, transdermal enhancer, wetting agent, surfactant and the antibacterial selectively, stir, make gel-type vehicle;

4) medicated powder, the step 2 that step 1) is obtained) gel-type vehicle that obtains of fine powder that obtains and step 3) mixes, and it is an amount of to add water, stirs, and is described gel.

The blue or green roc gel of the present invention is compared with the blue or green roc ointment of prior art has following advantage:

1, the present invention have no foreign body sensation, strong in the lesion tack, to skin mucosa nonirritant, advantage such as pollution clothes not.

2, the present invention also has advantages such as the convenience of smearing, uniform and smooth, no greasy feeling, Transdermal absorption speed be fast.

3, adopted micronizing, extraction process by water, alcohol extraction process to prepare active component among the present invention respectively, overcome the defective of traditional blue or green roc ointment preparation method, more effectively keep the active substance in the medical material and the active ingredient of medical material, improved the dissolution of crude drug composition greatly, dissolving, release, the stripping of medicine have been accelerated, improved bioavailability of medicament, made curative effect better.

4, in the present invention, about adopting the part material medicine to extract, be to utilize mechanical means, adopt the forced circulation mode, increasing solid-liquid contacts, the transmission of solute in solvent under dynamically in the medical material always keeps relative concentration poor, increased dissolving, the dissolution rate of medicinal ingredient, shortened the time, through extracting and separating effective ingredient, thereby reduced the drug administration amount, and improved therapeutic effect, also partly reduced the stripping of invalid components such as starch, colloid constituents simultaneously; With the preparation method that the surplus crude drug is pulverized, be influenced owing to effective ingredient in some medical material is subject to variation of temperature, can not adopt the method for extraction to carry out effective component extracting, so can only adopt the method for pulverizing.The present invention adopts crude drug partly to put forward the preparation method of half powder, has both kept effective ingredient in the medical material effectively, has improved the content of crude drug composition again greatly, has improved dissolution rate and bioavailability, makes curative effect better.

In order to understand essence of the present invention better,, describe in detail but do not limit the present invention below by description to better embodiment of the present invention.

Description of drawings

Fig. 1: the blue or green roc ointment transdermal experiment comparison diagram of blue or green roc gel of the present invention and prior art.

The specific embodiment

According to the specific embodiment of the present invention, the crude drug of gel of the present invention is formed and consumption can be preferably:

Herba Oxytropis Kansuensis 90-110 weight portion, sub-rhubarb extract 40-60 weight portion, Radix aconiti szechenyiani 60-90 weight portion, Fructus Chebulae (pitted) 90-110 weight portion, Fructus Terminaliae Billericae 90-110 weight portion, Fructus Phyllanthi 90-110 weight portion, Benzoinum 30-40 weight portion, Herba Lycopodii 140-160 weight portion, Moschus 20-30 weight portion.

More preferably, the crude drug of gel of the present invention composition and consumption are:

Herba Oxytropis Kansuensis 100 weight portions, sub-rhubarb extract 50 weight portions, Radix aconiti szechenyiani 75 weight portions, Fructus Chebulae (pitted) 100 weight portions, Fructus Terminaliae Billericae 100 weight portions, Fructus Phyllanthi 100 weight portions, Benzoinum 35 weight portions, Herba Lycopodii 150 weight portions, Moschus 25 weight portions.

Below, the plurality of raw materials medicine used to the present invention describes:

Herba Oxytropis Kansuensis is the dry herb of leguminous plant Herba Oxytropis falcatae Oxytropis falcata Bunge and Herba Oxytropis chiliophyllae O.chiliophyllaRoyle.Excavate herb at the beginning of autumn late summer, clean earth, Ex-all is assorted takes out, and dries.

Sub-rhubarb extract is the dry root and rhizome of plant Polygonaceae Radix Rhei emodi Rheum emodii Wall., Radix Rhei acuminati Rheumacuminatum Hook.f.et Thoms., Lhasa rhubarb Rheum lhasaense A.J.Li et P.K.Hsiao, the yellow Rheum inopinatum of red large pulse Prain, Tibet rhubarb Rheum tibeticum Maxim.ex Hook.f..

Radix aconiti szechenyiani is the dried root of cohosh FUMAO Radix aconiti szechenyiani Aconitum flavum Hand.-Mazz. and Radix aconiti szechenyiani A.pendulumBusch..Autumn end is excavated root, removes fibrous root and silt, dries.

Fructus Chebulae (pitted) this product is the dry mature fruit of Combretum Racemosum plant Fructus Chebulae (pitted) Terminalia chebula Retz. or fine hair Fructus Chebulae Terminaliachebula Retz.var.tomentella Kurt..Autumn, two season of winter gather during fruit maturation, remove impurity, dry.

Fructus Terminaliae Billericae adjoins the dry mature fruit of Li Le Terminalia billerica (Gaertn.) Roxb. for the Combretum Racemosum plant.Gather during fruit maturation winter, removes impurity, dries.

Fructus Phyllanthi is the dry mature fruit of euphorbia plant Fructus Phyllanthi Phyllanthus emblica L..Winter to time spring gathers during fruit maturation, removes impurity, drying.

Benzoinum is the dry resin of Styracaceae plant styrax tonkinensis Craib ex Hart Styrax tonkinensis (Pierre) Craib ex Hart..Trunk is collected effusive resin through damaging naturally or isolating trunk in summer, Qiu Erji, dries in the shade.

Herba Lycopodii is the dry stem of menispermaceous plants lobus cardiacus Herba Lycopodii Tinospora cordifolia (wulld) Miers or Herba Lycopodii T.sinensis (Lour.) Merr..Can adopt the whole year, cuts and get aerial parts, removes twig and leaf, and cutting is dried.

Moschus is the dry secretions in the ripe male body note capsule of animal in deer family woods Moschus moschiferous Moschus berezovskii Flerov, horse Moschus moschiferous MoschussifanicusPrzewalski or former Moschus moschiferous Moschus moschiferus Linnaeus.Moschus is divided into artificial Moschus and natural Moschus, and the artificial Moschus wins from the sachet of tame Moschus moschiferous, and the natural Moschus wins from the sachet of wild Moschus moschiferous.Wild Moschus moschiferous how hunt by the extremely inferior in the winter time spring, after trapping, extracts sachet, dries in the shade, and practises claiming " hair shell Moschus "; Cut sachet open, remove softgel shell, practise title " Moschus core ".The family Moschus moschiferous is directly taken out Moschus core from its sachet, dry in the shade or use the exsiccator close drying.Usually also the muscone of synthetic is called the artificial Moschus, its main pharmacological and natural Moschus are basic identical, and physical behavior is similar, and clinical efficacy is definite, can be equal to the prescription use with the natural Moschus.

Below specify the composition and the consumption of adjuvant in the gel of the present invention.In the present invention, 1 parts by volume: 1 weight portion=1L: 1kg.

According to the specific embodiment of the present invention, the adjuvant of gel of the present invention is formed and consumption can be preferably:

Gel skeleton material: carbomer 50-750 weight portion, sodium alginate 50-300 weight portion, poloxamer 50-550 weight portion, polyox-yethylene-polyoxypropylene block copolymer 80-280 weight portion, Polyethylene Glycol based block copolymer 50-400 weight portion, poly--2-ethoxy ethylene ether-poly-hydroxyethyl vinyl Ether block copolymer 40-300 weight portion, poly-N N-isopropylacrylamide 250-800 weight portion, polyacrylic acid 50-400 weight portion, sodium polyacrylate 100-300 weight portion, gelatin 80-1000 weight portion, methylcellulose 100-600 weight portion, ethyl cellulose 150-500 weight portion, sodium carboxymethyl cellulose 50-300 weight portion, chitosan 120-400 weight portion, remove in the acetyl-removed gellan gum 150-1600 weight portion one or more;

PH regulator: one or more in ammonia 0-300 weight portion, triethanolamine 0-350 weight portion, sodium hydroxide 0-600 weight portion, aluminium hydroxide 0-150 weight portion, sodium bicarbonate 0-180 weight portion, sodium hydrogen phosphate 0-180 weight portion and the sodium citrate 0-150 parts by volume;

Stabilizing agent: one or more in sodium pyrosulfite 0-350 weight portion, sodium sulfite 0-120 weight portion, sodium sulfite 0-180 weight portion, sodium thiosulfate 0-160 weight portion, ascorbic acid 0-90 weight portion, disodiumedetate (EDTA-2Na) 0-120 weight portion, ethylenediaminetetraacetic acid 0-260 weight portion, citric acid 0-410 weight portion and the tartaric acid 0-80 weight portion;

Transdermal enhancer: Borneolum Syntheticum 0-220 weight portion, Camphora 0-260 weight portion, Mentholum 0-120 weight portion, menthol 0-150 weight portion, methyl salicylate 0-230 weight portion, laurocapram 0-200 weight portion, Decylmethyl Sulphoxide 0-450 weight portion, plant Oleum sesami 0-140 weight portion;

Wetting agent: one or more in glycerol 0-550 weight portion, Polyethylene Glycol 0-500 weight portion, sorbitol 0-180 weight portion, propylene glycol 0-250 weight portion, paraffin oil 0-320 weight portion, mineral oil 0-65 weight portion, paraffin 0-165 weight portion, squalane 0-130 weight portion, silicone oil aliphatic alcohol 0-120 weight portion and the wax fat 0-165 weight portion

Surfactant: Polysorbate 0-200 weight portion, sodium lauryl sulphate 0-250 weight portion;

Antibacterial: one or more in parabens 0-30 weight portion, phenethanol 0-30 weight portion, sodium benzoate 0-15 weight portion and the sorbic acid 0-30 weight portion.

The adjuvant and the consumption of the blue or green roc gel of the present invention are preferably:

Gel skeleton material: carbomer 150-600 weight portion, sodium alginate 100-200 weight portion, poloxamer 150-450 weight portion, polyox-yethylene-polyoxypropylene block copolymer 150-200 weight portion, Polyethylene Glycol based block copolymer 150-300 weight portion, poly--2-ethoxy ethylene ether-poly-hydroxyethyl vinyl Ether block copolymer 100-200 weight portion, poly-N N-isopropylacrylamide 400-600 weight portion, polyacrylic acid 150-300 weight portion, sodium polyacrylate 150-250 weight portion, gelatin 300-800 weight portion, methylcellulose 200-400 weight portion, ethyl cellulose 250-400 weight portion, sodium carboxymethyl cellulose 100-250 weight portion, chitosan 200-300 weight portion, remove in the acetyl-removed gellan gum 500-1000 weight portion one or more;

PH regulator: one or more in ammonia 100-200 weight portion, triethanolamine 100-250 weight portion, sodium hydroxide 200-400 weight portion, aluminium hydroxide 50-100 weight portion, sodium bicarbonate 50-100 weight portion, sodium hydrogen phosphate 50-100 weight portion and the sodium citrate 50-100 parts by volume;

Stabilizing agent: one or more in sodium pyrosulfite 100-250 weight portion, sodium sulfite 30-90 weight portion, sodium sulfite 50-100 weight portion, sodium thiosulfate 50-100 weight portion, ascorbic acid 30-60 weight portion, disodiumedetate (EDTA-2Na) 30-90 weight portion, ethylenediaminetetraacetic acid 100-150 weight portion, citric acid 100-300 weight portion and the tartaric acid 20-60 weight portion;

Wetting agent: one or more in glycerol 150400 weight portions, Polyethylene Glycol 150-350 weight portion, sorbitol 50-100 weight portion, propylene glycol 50-150 weight portion, paraffin oil 150-250 weight portion, mineral oil 10-50 weight portion, paraffin 50-100 weight portion, squalane 30-100 weight portion, silicone oil aliphatic alcohol 20-100 weight portion and the wax fat 50-100 weight portion

Surfactant: Polysorbate 50-150 weight portion, sodium lauryl sulphate 50-200 weight portion;

Antibacterial: one or more in parabens 5-25 weight portion, phenethanol 5-25 weight portion, sodium benzoate 5-10 weight portion and the sorbic acid 5-25 weight portion.

Gel skeleton material: carbomer 400 weight portions, sodium alginate 175 weight portions, husky 300 weight portions in pool Lip river, polyox-yethylene-polyoxypropylene block copolymer 180 weight portions, Polyethylene Glycol based block copolymer 225 weight portions, poly--2-ethoxy ethylene ether-poly-hydroxyethyl vinyl Ether block copolymer 170 weight portions, poly-N N-isopropylacrylamide 525 weight portions, polyacrylic acid 225 weight portions, sodium polyacrylate 200 weight portions, gelatin 540 weight portions, methylcellulose 350 weight portions, ethyl cellulose 325 weight portions, sodium carboxymethyl cellulose 175 weight portions, chitosan 260 weight portions, remove in acetyl-removed gellan gum 875 weight portions one or more;

PH regulator: one or more in ammonia 150 weight portions, triethanolamine 175 weight portions, sodium hydroxide 300 weight portions, aluminium hydroxide 75 weight portions, sodium bicarbonate 90 weight portions, sodium hydrogen phosphate 90 weight portions and sodium citrate 75 parts by volume;

Stabilizing agent: one or more in sodium pyrosulfite 175 weight portions, sodium sulfite 60 weight portions, sodium sulfite 90 weight portions, sodium thiosulfate 80 weight portions, ascorbic acid 45 weight portions, disodiumedetate (EDTA-2Na) 60 weight portions, ethylenediaminetetraacetic acid 130 weight portions, citric acid 205 weight portions and tartaric acid 40 weight portions;

Transdermal enhancer: Borneolum Syntheticum 110 weight portions, Camphora 130 weight portions, Mentholum 60 weight portions, menthol 75 weight portions, methyl salicylate 115 weight portions, laurocapram 100 weight portions, Decylmethyl Sulphoxide 225 weight portions, plant Oleum sesami 70 weight portions;

Wetting agent: one or more in glycerol 0-275 weight portion, Polyethylene Glycol 250 weight portions, sorbitol 90 weight portions, propylene glycol 125 weight portions, paraffin oil 160 weight portions, mineral oil 32 weight portions, paraffin 83 weight portions, squalane 65 weight portions, silicone oil aliphatic alcohol 60 weight portions and wax fat 82 weight portions

Surfactant: Polysorbate 100 weight portions, sodium lauryl sulphate 125 weight portions;

Antibacterial: one or more in parabens 15 weight portions, phenethanol 15 weight portions, sodium benzoate 7 weight portions and sorbic acid 15 weight portions.

Gel skeleton material: carbomer 700 weight portions, sodium alginate 55 weight portions, poloxamer 500 weight portions, polyox-yethylene-polyoxypropylene block copolymer 85 weight portions, Polyethylene Glycol based block copolymer 390 weight portions, poly--2-ethoxy ethylene ether-poly-hydroxyethyl vinyl Ether block copolymer 45 weight portions, poly-N N-isopropylacrylamide 750 weight portions, polyacrylic acid 55 weight portions, sodium polyacrylate 295 weight portions, gelatin 85 weight portions, methylcellulose 550 weight portions, ethyl cellulose 155 weight portions, sodium carboxymethyl cellulose 295 weight portions, chitosan 125 weight portions, remove in acetyl-removed gellan gum 1500 weight portions one or more;

PH regulator: one or more in ammonia 10 weight portions, triethanolamine 340 weight portions, sodium hydroxide 50 weight portions, aluminium hydroxide 145 weight portions, sodium bicarbonate 10 weight portions, sodium hydrogen phosphate 175 weight portions and sodium citrate 10 parts by volume;

Stabilizing agent: one or more in sodium pyrosulfite 345 weight portions, sodium sulfite 10 weight portions, sodium sulfite 175 weight portions, sodium thiosulfate 10 weight portions, ascorbic acid 85 weight portions, disodiumedetate (EDTA-2Na) 10 weight portions, ethylenediaminetetraacetic acid 255 weight portions, citric acid 50 weight portions and tartaric acid 75 weight portions;

Transdermal enhancer: Borneolum Syntheticum 10 weight portions, Camphora 255 weight portions, Mentholum 10 weight portions, menthol 145 weight portions, methyl salicylate 20 weight portions, laurocapram 195 weight portions, Decylmethyl Sulphoxide 50 weight portions, plant Oleum sesami 135 weight portions;

Wetting agent: one or more in glycerol 500 weight portions, Polyethylene Glycol 50 weight portions, sorbitol 175 weight portions, propylene glycol 10 weight portions, paraffin oil 310 weight portions, mineral oil 10 weight portions, paraffin 160 weight portions, squalane 10 weight portions, silicone oil aliphatic alcohol 115 weight portions and wax fat 10 weight portions

Surfactant: Polysorbate 10 weight portions, sodium lauryl sulphate 245 weight portions;

Antibacterial: one or more in parabens 25 weight portions, phenethanol 5 weight portions, sodium benzoate 10 weight portions and sorbic acid 5 weight portions.

Gel skeleton material: carbomer 55 weight portions, sodium alginate 295 weight portions, poloxamer 55 weight portions, polyox-yethylene-polyoxypropylene block copolymer 275 weight portions, Polyethylene Glycol based block copolymer 55 weight portions, poly--2-ethoxy ethylene ether-poly-hydroxyethyl vinyl Ether block copolymer 295 weight portions, poly-N N-isopropylacrylamide 260 weight portions, polyacrylic acid 395 weight portions, sodium polyacrylate 110 weight portions, gelatin 950 weight portions, methylcellulose 110 weight portions, ethyl cellulose 495 weight portions, sodium carboxymethyl cellulose 55 weight portions, chitosan 395 weight portions, remove in acetyl-removed gellan gum 160 weight portions one or more;

PH regulator: one or more in ammonia 295 weight portions, triethanolamine 20 weight portions, sodium hydroxide 590 weight portions, aluminium hydroxide 10 weight portions, sodium bicarbonate 175 weight portions, sodium hydrogen phosphate 10 weight portions and sodium citrate 145 parts by volume;

Stabilizing agent: one or more in sodium pyrosulfite 20 weight portions, sodium sulfite 115 weight portions, sodium sulfite 10 weight portions, sodium thiosulfate 155 weight portions, ascorbic acid 10 weight portions, disodiumedetate (EDTA-2Na) 115 weight portions, ethylenediaminetetraacetic acid 20 weight portions, citric acid 400 weight portions and tartaric acid 10 weight portions;

Transdermal enhancer: Borneolum Syntheticum 215 weight portions, Camphora 20 weight portions, Mentholum 115 weight portions, menthol 10 weight portions, methyl salicylate 220 weight portions, laurocapram 10 weight portions, Decylmethyl Sulphoxide 440 weight portions, plant Oleum sesami 10 weight portions;

Wetting agent: one or more in glycerol 50 weight portions, Polyethylene Glycol 490 weight portions, sorbitol 10 weight portions, propylene glycol 245 weight portions, paraffin oil 20 weight portions, mineral oil 60 weight portions, paraffin 10 weight portions, squalane 125 weight portions, silicone oil aliphatic alcohol 10 weight portions and wax fat 160 weight portions

Surfactant: Polysorbate 195 weight portions, sodium lauryl sulphate 20 weight portions;

Antibacterial: one or more in parabens 5 weight portions, phenethanol 25 weight portions, sodium benzoate 1 weight portion and sorbic acid 25 weight portions.

The preparation method of the blue or green roc gel of the present invention comprises a kind of in A and the B method:

The A method:

1. Herba Oxytropis Kansuensis, sub-rhubarb extract, Radix aconiti szechenyiani, Fructus Chebulae, Fructus Terminaliae Billericae, Fructus Phyllanthi, Benzoinum, Herba Lycopodii are carried out remove impurity, cleaning, drying respectively, be ground into the medicated powder that particle diameter is 0.1-150 μ m;

2. Moschus is ground to form fine powder;

3. with gel skeleton material adding distil water, soaked overnight makes its abundant swelling, adds PH regulator, stabilizing agent, transdermal enhancer, wetting agent, surfactant and antibacterial, stirs, and makes gel-type vehicle;

4. after the gel-type vehicle that 3. makes of the medicated powder that above-mentioned steps is made in 1., Moschus fine powder and step that 2. step obtains mixed, it was an amount of to add water, stirs, and leaves standstill, and packing promptly gets gel of the present invention;

The B method:

1. with Herba Oxytropis Kansuensis, sub-rhubarb extract, Radix aconiti szechenyiani, Fructus Chebulae, Fructus Terminaliae Billericae, Fructus Phyllanthi, Benzoinum, Herba Lycopodii remove impurity, clean system, mixing;

2. Moschus is ground to form fine powder;

3. take by weighing the 10%-100% of above-mentioned mixed material medicine gross weight, decocting with the 5-20 times of weight boils 1-4 time, decocts collection, merge extractive liquid, and volatile oil 0.5-3 hour at every turn, it is the concentrated solution of 1.0-1.5 that filtration and concentrated extracting solution become relative density, gets concentrated solution a;

Or take by weighing the 10%-100% of above-mentioned mixed material medicine gross weight, decocting with the 5-20 times of weight boils 1-4 time, the each decoction 0.5-3 hour, collection, merge extractive liquid, and volatile oil filter, and the adsorption column of macroporous adsorbent resin is housed filtrate adding, 10-50 doubly measures eluting with 30-85% ethanol, collect eluent, be condensed into the concentrated solution that relative density is 1.0-1.5, and with volatile oil join in the concentrated solution concentrated solution a;

Or take by weighing the 10%-100% of above-mentioned mixed material medicine gross weight, and with the 30-90% alcohol reflux of 5-20 times of weight 1-4 time, extracted 0.5-24 hour at every turn, collect extracting solution, filtration, being condensed into relative density is the concentrated solution a of 1.0-1.5;

Or take by weighing the 10%-100% of above-mentioned mixed material medicine gross weight, the 30-90% ethanol that adds medicated powder weight 0.6-1.2 times of weight stirs and soaked into 0.5-2 hour, pack in the percolation jar, 30-90% ethanol percolation with the 5-20 times of weight, collect, merge percolate, filter, it is the concentrated solution a of 1.0-1.5 that concentrating under reduced pressure becomes relative density;

Or take by weighing the 10%-100% of above-mentioned mixed material medicine gross weight, and extract 1-4 time with the 30-90% alcohol dipping of 5-20 times of weight, flooded 12-24 hour at every turn, collect, merge impregnation liquid, filtration, it is the concentrated solution a of 1.0-1.5 that concentrating under reduced pressure becomes relative density;

4. will remain hybrid medicine and be ground into the medicated powder that particle diameter is 0.1-150 μ m;

5. with gel skeleton material adding distil water, soaked overnight makes its abundant swelling, adds PH regulator, stabilizing agent, transdermal enhancer, wetting agent, surfactant and antibacterial, stirs, and makes gel-type vehicle;

6. after the gel-type vehicle that 5. the Moschus fine powder that 2. above-mentioned steps is obtained, concentrated solution a and the step that 3. step obtains make mixed, it was an amount of to add water, stirs, and left standstill, and packing promptly gets gel of the present invention;

The A method:

1. Herba Oxytropis Kansuensis, sub-rhubarb extract, Radix aconiti szechenyiani, Fructus Chebulae, Fructus Terminaliae Billericae, Fructus Phyllanthi, Benzoinum, Herba Lycopodii are carried out remove impurity, cleaning, drying respectively, be ground into the superfine powder that particle diameter is 0.1-1 μ m;

2. Moschus is ground to form fine powder;

4. after the gel-type vehicle that 3. makes of the superfine powder that above-mentioned steps is made in 1., Moschus fine powder and step that 2. step obtains mixed, it was an amount of to add water, stirs, and leaves standstill, and packing promptly gets gel of the present invention;

The B method:

2. Moschus is ground to form fine powder;

3. take by weighing 10% of above-mentioned mixed material medicine gross weight, boil 3 times with 6 times of weight decoctings, decocted collection, merge extractive liquid, and volatile oil at every turn 1 hour, filter and concentrated extracting solution to become relative density be the concentrated solution of 1.25-1.35, and with volatile oil join in the concentrated solution concentrated solution a;

Or take by weighing 10% of above-mentioned mixed material medicine gross weight, decocting with 6 times of weight boils 3 times, the each decoction 1 hour, collection, merge extractive liquid, and volatile oil filter, and the adsorption column of macroporous adsorbent resin is housed filtrate adding, with 15 times of amounts of 35% ethanol eluting, collect eluent, be condensed into the concentrated solution that relative density is 1.25-1.35, and with volatile oil join in the concentrated solution concentrated solution a;

Or take by weighing 10% of above-mentioned mixed material medicine gross weight, and with 40% alcohol reflux of 6 times of weight 3 times, extracted 1 hour at every turn, collect, merge extractive liquid,, filter, being condensed into relative density is the concentrated solution a of 1.25-1.35;

Or taking by weighing 10% of above-mentioned mixed material medicine gross weight, 40% ethanol that adds medicated powder weight 0.8 times of weight stirs and soaked into 1 hour, in the percolation jar of packing into, ethanol percolation with 6 times of weight 40%, collect, merge percolate, filter, being evaporated to relative density is the concentrated solution a of 1.25-1.35;

Or take by weighing 10% of above-mentioned mixed material medicine gross weight, and extract 3 times with 40% alcohol dipping of 6 times of weight, flooded 16 hours at every turn, collect, merge impregnation liquid, filtering and be evaporated to relative density is the concentrated solution a of 1.25-1.35;

4. will remain hybrid medicine and be ground into the superfine powder that particle diameter is 0.1-1 μ m;

6. after the gel-type vehicle that 5. the Moschus fine powder that 2. above-mentioned steps is obtained, the concentrated solution a that 3. step obtains, superfine powder and step that 4. step obtains make mixed, it was an amount of to add water, stirs, and leaves standstill, and packing promptly gets gel of the present invention;

The A method:

1. Herba Oxytropis Kansuensis, sub-rhubarb extract, Radix aconiti szechenyiani, Fructus Chebulae, Fructus Terminaliae Billericae, Fructus Phyllanthi, Benzoinum, Herba Lycopodii are carried out remove impurity, cleaning, drying respectively, be ground into the superfine powder that particle diameter is 1-10 μ m;

2. Moschus is ground to form fine powder;

The B method:

2. Moschus is ground to form fine powder;

3. take by weighing 20% of above-mentioned mixed material medicine gross weight, boil 2 times with 8 times of weight decoctings, decocted collection, merge extractive liquid, and volatile oil at every turn 2 hours, filter and concentrated extracting solution to become relative density be the concentrated solution of 1.35-1.45, and with volatile oil join in the concentrated solution concentrated solution a;

Or take by weighing 20% of above-mentioned mixed material medicine gross weight, decocting with 8 times of weight boils 2 times, the each decoction 2 hours, collection, merge extractive liquid, and volatile oil filter, and the adsorption column of macroporous adsorbent resin is housed filtrate adding, with 20 times of amounts of 45% ethanol eluting, collect eluent, be condensed into the concentrated solution that relative density is 1.35-1.45, and with volatile oil join in the concentrated solution concentrated solution a;

Or take by weighing 20% of above-mentioned mixed material medicine gross weight, and with 50% alcohol reflux of 8 times of weight 2 times, extracted 2 hours at every turn, collect, merge extractive liquid,, filter, being condensed into relative density is the concentrated solution a of 1.35-1.45;

Or taking by weighing 20% of above-mentioned mixed material medicine gross weight, 50% ethanol that adds medicated powder weight 0.9 times of weight stirs and soaked into 1.5 hours, in the percolation jar of packing into, ethanol percolation with 8 times of weight 50%, collect, merge percolate, filter, being evaporated to relative density is the concentrated solution a of 1.0-1.5;

Or take by weighing 20% of above-mentioned mixed material medicine gross weight, and extract 2 times with 50% alcohol dipping of 8 times of weight, flooded 18 hours at every turn, collect, merge impregnation liquid, filtering and be evaporated to relative density is the concentrated solution a of 1.35-1.45;

4. will remain hybrid medicine and be ground into the superfine powder that particle diameter is 1-10 μ m;

6. after the gel-type vehicle that 5. the Moschus fine powder that 2. above-mentioned steps is obtained, the concentrated solution a that 3. step obtains, superfine powder and step that 4. step obtains make mixes, add water to the 300-600 weight portion, stir, leave standstill, packing promptly gets gel of the present invention;

The A method:

1. Herba Oxytropis Kansuensis, sub-rhubarb extract, Radix aconiti szechenyiani, Fructus Chebulae, Fructus Terminaliae Billericae, Fructus Phyllanthi, Benzoinum, Herba Lycopodii are carried out remove impurity, cleaning, drying respectively, be ground into the superfine powder that particle diameter is 10-40 μ m;

2. Moschus is ground to form fine powder;

The B method:

2. Moschus is ground to form fine powder;

3. take by weighing 50% of above-mentioned mixed material medicine gross weight, boil 2 times with 10 times of weight decoctings, decocted collection, merge extractive liquid, and volatile oil at every turn 1.5 hours, filter and concentrated extracting solution to become relative density be the concentrated solution of 1.15-1.25, and with volatile oil join in the concentrated solution concentrated solution a;

Or take by weighing 50% of above-mentioned mixed material medicine gross weight, decocting with 10 times of weight boils 2 times, the each decoction 1.5 hours, collection, merge extractive liquid, and volatile oil filter, and the adsorption column of macroporous adsorbent resin is housed filtrate adding, with 25 times of amounts of 50% ethanol eluting, collect eluent, be condensed into the concentrated solution that relative density is 1.15-1.25, and with volatile oil join in the concentrated solution concentrated solution a;

Or take by weighing 50% of above-mentioned mixed material medicine gross weight, and with 60% alcohol reflux of 10 times of weight 2 times, extracted 1.5 hours at every turn, collect extracting solution, filter, being condensed into relative density is the concentrated solution a of 1.15-1.25;

Or take by weighing 50% of above-mentioned mixed material medicine gross weight, 60% ethanol that adds medicated powder weight 1.0 times of weight stirs and soaked into 0.5 hour, pack in the percolation jar, ethanol percolation with 10 times of weight 60%, collect, merge percolate, filter, being evaporated to relative density is the concentrated solution a of 1.15-1.25;

Or take by weighing 50% of above-mentioned mixed material medicine gross weight, and extract 2 times with 60% alcohol dipping of 10 times of weight, flooded 20 hours at every turn, collect, merge impregnation liquid, filtering and be evaporated to relative density is the concentrated solution a of 1.15-1.25;

4. will remain hybrid medicine and be ground into the superfine powder that particle diameter is 10-40 μ m;

The A method:

1. Herba Oxytropis Kansuensis, sub-rhubarb extract, Radix aconiti szechenyiani, Fructus Chebulae, Fructus Terminaliae Billericae, Fructus Phyllanthi, Benzoinum, Herba Lycopodii are carried out remove impurity, cleaning, drying respectively, be ground into the medicated powder that particle diameter is 40-60 μ m;

2. Moschus is ground to form fine powder;

The B method:

2. Moschus is ground to form fine powder;

3. take by weighing 60% of above-mentioned mixed material medicine gross weight, boil 1 time with 12 times of weight decoctings, decocted collection, merge extractive liquid, and volatile oil 3 hours, filter and concentrated extracting solution to become relative density be the concentrated solution of 1.05-1.15, and with volatile oil join in the concentrated solution concentrated solution a;

Or take by weighing 60% of above-mentioned mixed material medicine gross weight, decocting with 12 times of weight boils 1 time, the each decoction 3 hours, collection, merge extractive liquid, and volatile oil filter, and the adsorption column of macroporous adsorbent resin is housed filtrate adding, with 30 times of amounts of 60% ethanol eluting, collect eluent, be condensed into the concentrated solution that relative density is 1.05-1.15, and with volatile oil join in the concentrated solution concentrated solution a;

Or take by weighing 60% of above-mentioned mixed material medicine gross weight, and with 75% alcohol reflux of 12 times of weight 1 time, extracted 24 hours, collect, merge extractive liquid,, filter, being condensed into relative density is the concentrated solution a of 1.05-1.15;

Or taking by weighing 60% of above-mentioned mixed material medicine gross weight, 75% ethanol that adds medicated powder weight 0.8 times of weight stirs and soaked into 1 hour, in the percolation jar of packing into, ethanol percolation with 12 times of weight 75%, collect, merge percolate, filter, being evaporated to relative density is the concentrated solution a of 1.05-1.15;

Or take by weighing 60% of above-mentioned mixed material medicine gross weight, and extract 1 time with 75% alcohol dipping of 12 times of weight, flooded 24 hours, collect, merge impregnation liquid, filtering and be evaporated to relative density is the concentrated solution a of 1.05-1.15;

4. will remain mixed material medicated powder and be broken into the medicated powder that particle diameter is 40-60 μ m;

6. after the gel-type vehicle that 5. the Moschus fine powder that 2. above-mentioned steps is obtained, the concentrated solution a that 3. step obtains, medicated powder and step that 4. step obtains make mixed, it was an amount of to add water, stirs, and leaves standstill, and packing promptly gets gel of the present invention;

The A method:

1. Herba Oxytropis Kansuensis, sub-rhubarb extract, Radix aconiti szechenyiani, Fructus Chebulae, Fructus Terminaliae Billericae, Fructus Phyllanthi, Benzoinum, Herba Lycopodii are carried out remove impurity, cleaning, drying respectively, be ground into the medicated powder that particle diameter is 60-90 μ m;

2. Moschus is ground to form fine powder;

The B method:

2. Moschus is ground to form fine powder;

3. take by weighing 90% of above-mentioned mixed material medicine gross weight, boil 4 times with 15 times of weight decoctings, decocted collection, merge extractive liquid, and volatile oil at every turn 0.5 hour, filter and concentrated extracting solution to become relative density be the concentrated solution of 1.15-1.25, and with volatile oil join in the concentrated solution concentrated solution a;

Or take by weighing 90% of above-mentioned mixed material medicine gross weight, decocting with 15 times of weight boils 4 times, the each decoction 0.5 hour, collection, merge extractive liquid, and volatile oil filter, and the adsorption column of macroporous adsorbent resin is housed filtrate adding, with 35 times of amounts of 70% ethanol eluting, collect eluent, be condensed into the concentrated solution that relative density is 1.15-1.25, and with volatile oil join in the concentrated solution concentrated solution a;

Or take by weighing 90% of above-mentioned mixed material medicine gross weight, and with 80% alcohol reflux of 15 times of weight 4 times, extracted 0.5 hour at every turn, collect extracting solution, filter, being condensed into relative density is the concentrated solution a of 1.15-1.25;

Or taking by weighing 90% of above-mentioned mixed material medicine gross weight, 80% ethanol that adds medicated powder weight 0.6 times of weight stirs and soaked into 5 hours, in the percolation jar of packing into, 80% ethanol percolation with 15 times of weight, collect, merge percolate, filter, being evaporated to relative density is the concentrated solution a of 1.15-1.25;

Or take by weighing 90% of above-mentioned mixed material medicine gross weight, and extract 4 times with 80% alcohol dipping of 15 times of weight, flooded 12 hours at every turn, collect, merge impregnation liquid, filtering and be evaporated to relative density is the concentrated solution a of 1.15-1.25;

4. will remain mixed material medicated powder and be broken into the medicated powder that particle diameter is 60-90 μ m;

The A method:

1. Herba Oxytropis Kansuensis, sub-rhubarb extract, Radix aconiti szechenyiani, Fructus Chebulae, Fructus Terminaliae Billericae, Fructus Phyllanthi, Benzoinum, Herba Lycopodii are carried out remove impurity, cleaning, drying respectively, be ground into the medicated powder that particle diameter is 90-150 μ m;

2. Moschus is ground to form fine powder;

The B method:

2. Moschus is ground to form fine powder;

3. take by weighing above-mentioned mixed material medicine, boil 3 times with 10 times of weight decoctings, decocted collection, merge extractive liquid, and volatile oil at every turn 2 hours, filter and concentrated extracting solution to become relative density be the concentrated solution of 1.05-1.15, and with volatile oil join in the concentrated solution concentrated solution a;

Or take by weighing above-mentioned mixed material medicine, decocting with 10 times of weight boils 3 times, the each decoction 2 hours, collection, merge extractive liquid, and volatile oil filter, and the adsorption column of macroporous adsorbent resin is housed filtrate adding, with 40 times of amounts of 80% ethanol eluting, collect eluent, be condensed into the concentrated solution that relative density is 1.05-1.15, and with volatile oil join in the concentrated solution concentrated solution a;

Or take by weighing above-mentioned mixed material medicine, and use 60% alcohol reflux 3 times of 10,8,6 times of medicine gross weights respectively, extracted respectively 90,60,30 minutes at every turn, collect, merge extractive liquid,, filter, being condensed into relative density is the concentrated solution a of 1.05-1.15;

Or taking by weighing above-mentioned mixed material medicine, 60% ethanol that adds medicated powder weight 1.0 times of weight stirs and soaked into 1 hour, in the percolation jar of packing into, 60% ethanol percolation with 10 times of weight, collect, merge percolate, filter, being evaporated to relative density is the concentrated solution a of 1.05-1.15;

Or take by weighing above-mentioned mixed material medicine, and extract 3 times with 60% alcohol dipping of 10 times of weight, flooded 18 hours at every turn, collect, merge impregnation liquid, filtering and be evaporated to relative density is the concentrated solution a of 1.05-1.15;

4. with gel skeleton material adding distil water, soaked overnight makes its abundant swelling, adds PH regulator, stabilizing agent, transdermal enhancer, wetting agent, surfactant and antibacterial, stirs, and makes gel-type vehicle;

5. after the gel-type vehicle that 4. the Moschus fine powder that 2. above-mentioned steps is obtained, concentrated solution a and the step that 3. step obtains make mixed, it was an amount of to add water, stirs, and left standstill, and packing promptly gets gel of the present invention.

The used test material of the present invention if no special instructions, is commercially available purchase product.

Following experimental example and embodiment are used to further specify blue or green roc gel of the present invention, but the invention is not restricted to this.

Experimental example 1: gel adjuvant screening experiment of the present invention

1, the optimization test of gel-type vehicle

1. the selection of gel-type vehicle

Take by weighing Herba Oxytropis Kansuensis 100g, sub-rhubarb extract 50g, Radix aconiti szechenyiani 75g, Fructus Chebulae 100g, Fructus Terminaliae Billericae 100g, Fructus Phyllanthi 100g, Benzoinum 35g, Herba Lycopodii 150g, Moschus 25g; Moschus is ground to form fine powder; All the other medical materials decoct 3 times with 10 times of water of medical material gross weights, decocted 2 hours at every turn, collection, merge extractive liquid, and volatile oil, filter and concentrated extracting solution to become relative density be the concentrated solution of 1.05-1.15, and volatile oil is joined in the concentrated solution must concentrated solution a; Take by weighing the 500g gel-type vehicle respectively, add an amount of distilled water, soaked overnight makes its abundant swelling, and adding laurocapram 45g, glycerol 72g, ethyl hydroxybenzoate 2.5g make gel-type vehicle; Superfine powder, Moschus fine powder are joined in the gel-type vehicle, add water to 900g, stir, leave standstill, gel outward appearance situation is observed in packing, the results are shown in Table 1.

The different gel outward appearance of table 1 situation result

Experimental result shows, arabic gum, polyoxyethylene, liquid paraffin effect are bad, wherein carbomer, gelatin, sodium alginate, sodium carboxymethyl cellulose, polyox-yethylene-polyoxypropylene block copolymer, Polyethylene Glycol based block copolymer, poly--2-ethoxy ethylene ether-poly-hydroxyethyl vinyl Ether block copolymer, go the acetyl-removed gellan gum effect to carry out.

2. the selection of gel-type vehicle consumption

According to the form below 2 design tables are tested, and make the gel of different gel-type vehicles, observe gel outward appearance situation, the results are shown in Table 3.

The different gel-type vehicle EXPERIMENTAL DESIGN of table 2 scheme table

The different gel outward appearance of table 3 situation result

Annotate: the uniformity that is meant gel well is fine, viscosity is fine, sophistication good;

Be meant that well the having good uniformity of gel, viscosity are good, fine and smooth good;

The bad lack of homogeneity that is meant gel, viscosity is general, sophistication is poor.

Experimental result shows, the optimum amount of gel-type vehicle is: the 0.3-0.9 of carbomer comprises crude drug doubly, the 0.4-1.0 that gelatin accounts for crude drug doubly, sodium alginate account for crude drug 0.2-0.4 doubly, sodium carboxymethyl cellulose account for crude drug 0.2-0.4 doubly, polyox-yethylene-polyoxypropylene block copolymer account for crude drug 0.2-0.4 doubly, the Polyethylene Glycol based block copolymer account for crude drug 0.2-0.4 doubly, poly--2-ethoxy ethylene ether-poly-hydroxyethyl vinyl Ether block copolymer account for crude drug 0.2-0.4 doubly, go 0.3-1.5 that acetyl-removed gellan gum accounts for crude drug doubly.

2, the optimization test of transdermal enhancer:

1. the selection of transdermal enhancer

Take by weighing Herba Oxytropis Kansuensis 100g, sub-rhubarb extract 50g, Radix aconiti szechenyiani 75g, Fructus Chebulae 100g, Fructus Terminaliae Billericae 100g, Fructus Phyllanthi 100g, Benzoinum 35g, Herba Lycopodii 150g, Moschus 25g; Moschus is ground to form fine powder; All the other medical materials decoct 3 times with 10 times of water of medical material gross weights, decocted 2 hours at every turn, collection, merge extractive liquid, and volatile oil, filter and concentrated extracting solution to become relative density be the concentrated solution of 1.05-1.15, and volatile oil is joined in the concentrated solution must concentrated solution a; Take by weighing carbomer 375g and add an amount of distilled water, soaked overnight makes its abundant swelling, adds glycerol 72g, ethyl hydroxybenzoate 2.5g and each 50g of different types of transdermal enhancer, makes gel-type vehicle; Superfine powder, Moschus fine powder are joined in the gel-type vehicle, add water to 1000g, stir, leave standstill, drug transdermal absorbing state is measured in packing.

The present invention adopts the transdermal test method that exsomatizes, and gets white mice, etherization, carefully cut off back wool with shears, put to death, peel off skin of back, remove subcutaneous fat and mucous tissue, after cleaning with physiological saline solution, place the joint portion of the vertical diffusion cell of Franz, dermis of skin is towards receiving liquid, accurately take by weighing the gel of above-mentioned different transdermal enhancers, evenly coat skin surface, reception tank adds 30ml receiver media (constant temperature (32 ± 1) ℃, mixing speed 100r/min).Respectively at 1,3,6,9,12,24h sampling 2ml (replenishing receiver media 2ml simultaneously) puts in the 25ml measuring bottle, is diluted to scale with methanol solution, as need testing solution, operate with method with blank sample, make blank solution, measure trap in 269nm wavelength place, the results are shown in Table 4.

Table 4 gallic acid accumulative total transit dose result

Used transdermal enhancer all has and significantly sees through effect among the present invention, wherein laurocapram, Mentholum, that Decylmethyl Sulphoxide sees through effect is best.

2. the selection of transdermal enhancer consumption

According to the form below 5 design tables are tested, make the gel of different transdermal enhancers, adopt the transdermal test method that exsomatizes, get white mice, etherization, carefully cut off back wool with shears, put to death, peel off skin of back, remove subcutaneous fat and mucous tissue, after cleaning with physiological saline solution, place the joint portion of the vertical diffusion cell of Franz, dermis of skin is towards receiving liquid, accurately take by weighing the gel of above-mentioned different transdermal enhancers, evenly coat skin surface, reception tank adds 30ml receiver media (constant temperature (32 ± 1) ℃, mixing speed 100r/min).Respectively at 1,3,6,9,12,24h sampling 2ml (replenishing receiver media 2ml simultaneously) puts in the 25ml measuring bottle, is diluted to scale with methanol solution, as need testing solution, operate with method with blank sample, make blank solution, measure trap in 269nm wavelength place, the results are shown in Table 6.

Table 5 transdermal enhancer EXPERIMENTAL DESIGN scheme table

Table 6 gallic acid transit dose result of the test

Experimental result shows that the optimum amount of transdermal enhancer is: the 0.1-0.25 that laurocapram accounts for crude drug doubly, the 0.05-0.15 that Mentholum accounts for crude drug doubly, Decylmethyl Sulphoxide account for crude drug 0.15-0.35 doubly.

3, the optimization test of wetting agent:

1. the selection of wetting agent

Take by weighing Herba Oxytropis Kansuensis 100g, sub-rhubarb extract 50g, Radix aconiti szechenyiani 75g, Fructus Chebulae 100g, Fructus Terminaliae Billericae 100g, Fructus Phyllanthi 100g, Benzoinum 35g, Herba Lycopodii 150g, Moschus 25g; Moschus is ground to form fine powder; All the other medical materials decoct 3 times with 10 times of water of medical material gross weights, decocted 2 hours at every turn, collection, merge extractive liquid, and volatile oil, filter and concentrated extracting solution to become relative density be the concentrated solution of 1.05-1.15, and volatile oil is joined in the concentrated solution must concentrated solution a; Take by weighing the 120g carbomer and add an amount of distilled water, soaked overnight makes its abundant swelling, adds laurocapram 30g, ethyl hydroxybenzoate 2.5g and each 50g of different types of wetting agent, makes gel-type vehicle; Superfine powder, Moschus fine powder are joined in the gel-type vehicle, add water to 500g, stir, leave standstill, packing, room temperature is placed, and observes medicine moisture retention situation in 3-5 days.(the results are shown in Table 7)

The moistening effect of the various wetting agents of table 7 relatively

Annotate: bad: skin surface is dry and sense of discomfort arranged;

Good: the skin surface moistening does not have sense of discomfort;

Better: the basic moistening of skin surface and sense of discomfort is arranged slightly;

Used wetting agent all has tangible moisture-keeping function among the present invention, and wherein the moisture-keeping function of glycerol, Polyethylene Glycol, Sorbitol, propylene glycol is best.

2. the selection of wetting agent consumption

According to the form below 8 design tables are tested, and make the gel of different wetting agents, and room temperature is placed, and observe medicine moisture retention situation in 3-5 days, the results are shown in Table 9.

Table 8 wetting agent is investigated the design table

Table 9 moistening effect result

Experimental result shows that the optimum amount of wetting agent is: the 0.2-0.6 that glycerol accounts for crude drug doubly, the 0.1-0.3 that propylene glycol accounts for crude drug doubly, Polyethylene Glycol account for crude drug 0.3-0.6 doubly, sorbitol account for crude drug 0.05-0.2 doubly.

Experimental example 2: blue or green roc gel of the present invention and the test of blue or green roc ointment Transdermal absorption are relatively

1, test specimen:

The blue or green roc ointment of tradition: XiZang QiZheng Tibetan pharmaceuticals Co., Ltd provides;

Blue or green roc ointment 1: preparation method: 1. Herba Oxytropis Kansuensis 100g, sub-rhubarb extract 50g, Radix aconiti szechenyiani 75g, Fructus Chebulae 100g, Fructus Terminaliae Billericae 100g, Fructus Phyllanthi 100g, Benzoinum 35g, Herba Lycopodii 150g are made only, pulverize, mix homogeneously, standby; 2. take by weighing 50% of above-mentioned mixed material medicine gross weight,, decoct 3 times down, fried in shallow oil 2 hours at every turn, collect extracting solution and volatile oil respectively in 85 ℃ with the water of 2.13kg; Filter, concentrated extracting solution is 1.0～1.5 concentrated solution to relative density; Volatile oil is joined in the concentrated solution; 3. above-mentioned remaining mixed material medicated powder is broken into the medicated powder of 100 μ m, mixes, get mixed liquor a with the above-mentioned concentrated solution that contains volatile oil; Mixed liquor a is joined in the water of 50 times of weight, stir, obtain mixed liquor b; 4. mixed liquor b is mixed with 2: 1: 1 weight ratio with liquid paraffin and Tween-80, emulsifying gets emulsifying ointment; 5. the Moschus of 25g is joined in the water of 375 grams and grinds, cross 150 mesh sieves after, join in the above-mentioned emulsifying ointment and stir, promptly get QINGPENG GAOJI;

Blue or green roc ointment 2: preparation method: 1. Herba Oxytropis Kansuensis 13.5g, sub-rhubarb extract 6.5g, Radix aconiti szechenyiani 10.5g, Fructus Chebulae 13.5g, Fructus Terminaliae Billericae 13.5g, Fructus Phyllanthi 13.5g, Benzoinum 4.5g, Herba Lycopodii 20.5g are made only, pulverize, mix homogeneously, standby; 2. take by weighing 50% of mixed powder gross weight, the alcohol reflux with 50% 1 time extracted 1.5 hours, and alcohol adding amount is 20 times of medical material gross weight, and 50 ℃ of temperature are collected extracting solution; Filter, be condensed into proportion and be 1.0 concentrated solution, standby; 3. will remain medicated powder (50%) be ground into the medicated powder of 0.01～200 μ m after, mix with concentrated medicament, 45g octadecanol, 200g water, stir, obtain the mixed liquor of medicinal liquid and water, be warming up to 75 ℃ standby; 4. get the 100g lanoline, gained mixed liquor in adding 3. when being warming up to 75 ℃ stirs, and is to place in the homogenizer under the condition of 0.09MPa to stir 20 minutes fast keeping vacuum pressure, treat that temperature reduces to 30 ℃, add and promptly make ointment after Moschus 3.5g stirs;

Blue or green roc ointment 3: preparation method: 1. Moschus 3.4g is ground to form fine powder; 2. Herba Oxytropis Kansuensis, sub-rhubarb extract, Radix aconiti szechenyiani, Fructus Chebulae, Fructus Terminaliae Billericae, Fructus Phyllanthi, Benzoinum, Herba Lycopodii are picked totally, got Herba Oxytropis Kansuensis 13.6g, sub-rhubarb extract 6.8g, Radix aconiti szechenyiani 10.2g, Fructus Chebulae 13.6g, Fructus Terminaliae Billericae 13.6g, Fructus Phyllanthi 13.6g, Benzoinum 4.7g, Herba Lycopodii 20.4g and mix the back micronizing to become particle diameter be 0.01-0.1 μ m micropowder; 3. be mixed and made into ointment base with Tween-80 42g, liquid paraffin 16.8g, glycerol 2g, water 50g; 4. with technology 1. and 2. medicated powder mix with ointment base, make ointment, discharging, packing are promptly;

The blue or green roc gel of the present invention 1 (embodiment 12 is prepared from the description according to the present invention).

2, the preparation of isolated skin

The disconnected neck of nude mice is put to death, and strips skin of back and measures whole bark thickness, wraps standby then with preservative film.

3, transdermal experiment and test sample

Whole bark is placed the joint portion (volume is 10ml, and effective area is 1.6cm2) of the vertical diffusion cell of Franz, 3 kinds of samples are respectively got 2g, be applied in skin surface respectively.Receive liquid and adopt 1% potassium dihydrogen phosphate.(1,3,6,9,12,24h) the each 400 μ l of sampling replenish the release medium with volume at setting-up time respectively.The accumulative total transit dose is calculated by following formula:

Q＝(C _n×V+∑C _n-1×0.15)/A

Q: the unit are accumulation sees through dose; V: it is long-pending to receive liquid; A: diffusion cell open area; The concentration of Cn n sub-sampling.Test triplicate at least at every turn.

4, reference substance preparation

Precision takes by weighing the gallic acid reference substance of drying under reduced pressure to constant weight, adds the solution that methanol is made 0.108mg/mL, in contrast product solution.

5, the chromatographic condition of marker ingredients Carthamus yellow and system suitability:

High performance liquid chromatography (HPLC) analytical method

Chromatographic column Kromasil C18 250 * 4.6mm 5 μ m;

Mobile phase: methanol one 0.025% phosphate aqueous solution (4:96);

Flow velocity 1mL/min;

Detect wavelength 275nm;

Column temperature: 25 ℃

Sample size: 10 μ L;

Analytical method: external standard method.

6, experimental result and discussion

Experimental result is seen Fig. 1.The result shows that in 24 hours, gallic acid accumulative total transit dose is compared with gallic acid accumulative total transit dose in the blue or green roc ointment of tradition, blue or green roc ointment 1, blue or green roc ointment 2, the blue or green roc ointment 3 in the blue or green roc gel of the present invention, and the skin transit dose has improved nearly twice.

Experimental example 3: the blue or green roc gel of the present invention suppresses the experimentation of rat gouty arthritis

1, reagent, animal and reagent

(1) reagent

The blue or green roc gel (embodiment 2 is prepared from the description according to the present invention) of the present invention.

(2) animal

The SD rat, male, 80, body weight 190～210g is provided by Beijing dimension tonneau China laboratory animal technology company limited, the quality certification number: SCXK (capital) 2008-0003.

(3) reagent

Indomethacin (etidocine tablet), Zhangjiakou City east wind pharmaceutical factory, lot number 20070703; Uric acid, Sigma, packing factory of Shanghai chemical reagents corporation, lot number, 20401; The preparation of micro-crystal type uric acid sodium (MSU): get the 4g uric acid, place 800ml boiling water, transfer pH to 7.4, reheat to 95 ℃, the room temperature cooling is also stirred gently, filters, get micro-crystal type uric acid sodium (MSU), high temperature sterilize (200 ℃) is with the preceding suspension that is mixed with 100mg/ml with physiological saline solution.

2, method and result

(1) method

80 of male SD rats, be divided into 8 groups at random by body weight, every group 10, be respectively: the I group is model control group, II organizes positive matched group (indomethacin 6.75mg/kg/d), the III group is traditional blue or green roc ointment group (0.48g (crude drug)/kg/d), the IV group is 1 group of (0.48g (crude drug)/kg/d) of blue or green roc ointment, the V group is 2 groups of (0.48g (crude drug)/kg/d) of blue or green roc ointment, the VI group is 3 groups of (0.48g (crude drug)/kg/d) of blue or green roc ointment, (0.12g (crude drug)/kg/d), the VIII group is the blue or green roc gel of the present invention high dose group (0.48g (crude drug)/kg/d) to the VII group for the blue or green roc gel of the present invention low dose group.Wherein positive drug is a gastric infusion, and all the other are percutaneous dosing, 7 days (every day 1 time) of successive administration, behind the last administration 1h, at micro-crystal type uric acid sodium (MSU) the 100 μ l/ of its right hind foot sole of the foot subcutaneous injection 100mg/ml of portion only, respectively at injection back 1h, 2h, 4h, 6h, 8h calculates the foot swelling rate with miking swelling foot sole of the foot portion thickness, organize a t check, the results are shown in Table 10.

Computing formula is as follows:

Foot swelling rate=(injection metapedes sole of the foot thickness-injection front foot sole of the foot thickness)/injection front foot sole of the foot thickness * 100%

Table 10 gel of the present invention to micro-crystal type uric acid sodium (MSU) cause rat paw edema influence (x ± s, n=10)

Annotate: each administration group and model control group compare: ¹P＜0.05, ²P＜0.01, traditional blue or green roc ointment group, 1 group of blue or green roc ointment, 2 groups of blue or green roc ointment, 3 groups of blue or green roc ointment, the blue or green roc gel of the present invention low dose group are compared with the blue or green roc gel of the present invention high dose group respectively: ³P＜0.05, ⁴P＜0.01.

(2) statistical analysis

Each organizes rat paw edema rate mean ± standard deviation

Expression adopts the SPSS10.0 statistical software to carry out date processing.Carry out one factor analysis of variance, check with LSD during homogeneity of variance, heterogeneity of variance carries out comparing between each group with Dunnett ' s T3 check, P＜0.05, and P＜0.01 expression difference has significance.

(3) result

Experimental result is as shown in Table 10: traditional blue or green roc ointment group, 1 group of blue or green roc ointment, 2 groups of blue or green roc ointment, 3 groups of blue or green roc ointment, the blue or green roc gel of the present invention low dose group, the blue or green roc gel of the present invention high dose group are suppressing micro-crystal type uric acid sodium vola injection back 1h, 2h, 4h, 6h, 8h causes rat paw edema with model matched group significant difference (P＜0.01 of having compared, P＜0.05), all has certain antiinflammatory action.1,2,3 groups of tradition blue or green roc ointment group, blue or green roc ointment are with the blue or green roc gel of the present invention high dose group 1h behind vola injection micro-crystal type uric acid sodium, 2h, 4h, 6h, 8h causes rat paw edema rate significant difference (P＜0.01 of having compared, P＜0.05), it is better that the blue or green roc gel of the present invention high dose group presses down swollen rate.Each time point of the blue or green roc gel of the present invention low dose group records presses down swollen rate with the blue or green roc gel of the present invention high dose group significant difference (P＜0.01) of having compared.In a word, the more traditional blue or green roc ointment group of antiinflammatory action of the blue or green roc gel of the present invention, 1,2,3 groups of blue or green roc ointment are better, and the blue or green roc gel of the present invention high dose group is compared with the blue or green roc gel of the present invention group, and antiinflammatory action has dose dependent.

Experimental example 4: the blue or green roc gel of the present invention suppresses the experimentation of mice caused by dimethylbenzene xylene auricle edema

1, reagent, animal and instrument and reagent

(1) reagent

Blue or

green roc ointment

1,2 and 3 is prepared from method described in the above-mentioned experimental example 3;

The blue or green roc gel (embodiment 15 is prepared from the description according to the present invention) of the present invention.

(2) animal

KM kind mice, 80, male and female half and half, body weight 20 ± 2g is provided by Lanzhou Institute of Biological Products's animal center, the quality certification number: the moving word 08-014 of doctor.

(3) instrument and reagent

Ten thousand/electronic analytical balance, Beijing Sai Duolisi balance company limited; Electronic balance, Haikang, Shanghai Electronic Instruments Plant; Microsyringe (0.05ml), Shanghai Medicine Laser Instrument Plant; Stopwatch, Shenzhen epoch Powerise digital technology Co., Ltd, the 1ml syringe; Card punch (8mm); Dexamethasone acetate tablets, Zhejiang Province XianJu Pharmacy stock Co., Ltd, lot number: 080911; Dimethylbenzene, Xi'an chemical reagent factory, lot number: 010922.

2, method and result

(1) method

Choose 80 of healthy KM kind mices, be divided into 8 groups at random by body weight, every group 10, be respectively: the I group is model control group, II organizes positive matched group (dexamethasone 6mg/kg/d), the III group is traditional blue or green roc ointment group (0.6g (crude drug)/kg/d), the IV group is 1 group of (0.6g (crude drug)/kg/d) of blue or green roc ointment, the V group is 2 groups of (0.6g (crude drug)/kg/d) of blue or green roc ointment, the VI group is 3 groups of (0.6g (crude drug)/kg/d) of blue or green roc ointment, (0.15g (crude drug)/kg/d), the VIII group is the blue or green roc gel of the present invention high dose group (0.6g (crude drug)/kg/d) to the VII group for the blue or green roc gel of the present invention low dose group.Respectively organize continuous percutaneous dosing six days before the experiment, model control group gives isopyknic purified water, 30min after the last administration, with warm water with the auris dextra scrub, drawing 0.03ml/ dimethylbenzene with microsyringe evenly smears on the mouse right ear two sides and carries out proinflammatory effect, left side ear in contrast, the dislocation of animal cervical vertebra is put to death after dripping proinflammatory agent 30min, cut left and right sides auricle along the helix exterior feature, sweep away auricle with the card punch of diameter 8mm, weigh with analytical balance at the same corresponding site of ears, with two auricle weight differences is the swelling level index, compare each group difference, and obtain and press down swollen rate, the results are shown in Table 11.

Swelling degree (mg)=auris dextra weight-left ear weight

Press down swollen rate (%)=(model control group swelling degree-administration group swelling degree)/model control group swelling degree * 100%

Table 11 gel xylol of the present invention cause mice auricle swelling inhibitory action ( N=10)

(2) statistical analysis

Each organizes mice auricle swelling degree mean ± standard deviation

(3) result

Experimental result is as shown in Table 11: traditional blue or green roc ointment group, 1 group of blue or green roc ointment, 2 groups of blue or green roc ointment, 3 groups of blue or green roc ointment, the blue or green roc gel of the present invention low dosage agent group, the blue or green roc high dose of the present invention gel group all can suppress the mice caused by dimethylbenzene xylene ease auricle swelling, have certain antiinflammatory action.1,2,3 groups of tradition blue or green roc ointment group, blue or green roc ointment to press down swollen rate be 24.40%, 32.74%, 35.71%, 30.95%, auricle swelling degree relatively has with the model matched group and significant difference is all arranged (P＜0.01); Wherein blue or green roc ointment 2 effects are better.The auricle swelling degree of the blue or green roc ointment group of tradition more all has significant difference (P＜0.01) with the blue or green roc gel of the present invention high dose group (pressing down swollen rate is 42.26%); 1,2,3 groups of auricle swelling degrees of blue or green roc ointment compare there was no significant difference (P＞0.05) with the blue or green roc gel of the present invention high dose group (pressing down swollen rate is 42.26%); The blue or green roc gel of the present invention low dose group to press down swollen rate be 20.24%, its auricle swelling degree is with the blue or green roc gel high dose group significant difference (P＜0.05) of having compared.In a word, the blue or green roc gel of the present invention is compared for 3 groups with traditional blue or green roc ointment group, 1 group of blue or green roc ointment, 2 groups of blue or green roc ointment, blue or green roc ointment, and antiinflammatory action is better, and the blue or green roc gel of the present invention antiinflammatory action has dose dependent.

Experimental example 5: the blue or green roc gel of the present invention suppresses the experimentation that mouse peritoneal injection acetic acid causes pain and the rising of abdominal cavity capillary permeability

1, reagent, animal and reagent

(1) reagent

Blue or

green roc ointment

The blue or green roc gel (embodiment 24 is prepared from the description according to the present invention) of the present invention.

(2) animal

Mice, the Kunming kind, 80, male, body weight 18～22g is provided by Beijing dimension tonneau China laboratory animal technology company limited, the quality certification number: SCXK (capital) 2005-0005.

(3) reagent

Aspirin, Xi'an Bo Hua Pharmaceutical Co, lot number 0102019; Acetic acid, analytical pure, Beijing Chemical Plant, lot number 20060807.

2, method and result

(1) method

Choose 80 of healthy KM kind mices, be divided into 8 groups at random by body weight, every group 10, be respectively: the I group is model control group, II organizes positive matched group (aspirin 100mg/kg/d), the III group is traditional blue or green roc ointment group (0.6g (crude drug)/kg/d), the IV group is 1 group of (0.6g (crude drug)/kg/d) of blue or green roc ointment, the V group is 2 groups of (0.6g (crude drug)/kg/d) of blue or green roc ointment, the VI group is 3 groups of (0.6g (crude drug)/kg/d) of blue or green roc ointment, (0.15g (crude drug)/kg/d), the VIII group is the blue or green roc gel of the present invention high dose group (0.6g (crude drug)/kg/d) to the VII group for the blue or green roc gel of the present invention low dose group.Each is organized mice and all feeds under the same conditions, freely ingests, takes the photograph water, and room temperature is controlled at 20 ± 1 ℃, and humidity is about 60%, and wherein positive drug is a gastric infusion, and all the other are percutaneous dosing, are administered six times altogether.Behind the last administration 1h, lumbar injection 0.6% acetum 0.2ml/ only writes down mouse writhing number of times in the 15min (the abdominal part indent stretches hind leg, and buttocks is raised), calculates medicine is passed judgment on pharmaceutical composition to the suppression ratio of turning round body action effect.The blue normal saline solution 0.1ml/10g of tail vein injection 0.5% ivens body weight behind lumbar injection acetic acid 30min then, after 20min mice being taken off vertebra puts to death, an intraperitoneal injection normal saline 6ml, gently rub mouse web portion, so that the intraperitoneal dyestuff fully is dissolved in the normal saline and mix homogeneously, puncture into the abdominal cavity with No. 6 syringe needles then, the careful 4ml liquid of drawing, every pipe centrifugal 3000rpm, behind the 10min, get supernatant and measure the optical density at 590nm place, be converted into dyestuff content, the results are shown in Table 12 according to standard curve.

Table 12 gel of the present invention is to the influence of mice acetic acid twisting (x ± s)

(2) statistical analysis

Each organizes mouse writhing number of times and peritoneal exudate dyestuff content mean ± standard deviation Expression adopts the SPSS10.0 statistical software to carry out date processing.Carry out one factor analysis of variance, check with LSD during homogeneity of variance, heterogeneity of variance carries out comparing between each group with Dunnett ' s T3 check, P＜0.05, and P＜0.01 expression difference has significance.

(3) result

Experimental result is as shown in table 12: traditional blue or green roc ointment group, 1 group of blue or green roc ointment, 2 groups of blue or green roc ointment, 3 groups of blue or green roc ointment, the blue or green roc gel of the present invention group, the blue or green roc gel of the present invention group all can suppress acetic acid and cause the mouse writhing number of times, reduce peritoneal exudate dyestuff content, has the effect that the certain analgesia and the inflammation-inhibiting factor are oozed out, the blue or green roc ointment group of tradition, 1 group of blue or green roc ointment, 2 groups of blue or green roc ointment, 3 groups of blue or green roc ointment, the blue or green roc gel of the present invention low dose group, the blue or green roc gel of the present invention high dose agent group suppresses acetic acid and causes mouse writhing number of times and the model control group significant difference (P＜0.01) of having compared, the blue or green roc gel of tradition blue or green roc ointment group and the present invention low dose group suppresses acetic acid and causes mouse writhing number of times and the blue or green roc gel of the present invention high dose group significant difference (P＜0.01 of having compared, P＜0.05), blue or green roc ointment 1,2,3 groups are suppressed that acetic acid causes the mouse writhing number of times and the blue or green roc gel of the present invention high dose group is compared, it is worth lower slightly but there was no significant difference (P＞0.05), the abdominal cavity is not oozed out amount of dye and is suppressed acetic acid with it and cause the mouse writhing number of times and compare and have certain dependency on the same group, the blue or green roc ointment group of tradition, 1 group of blue or green roc ointment, 2 groups of blue or green roc ointment, 3 groups of blue or green roc ointment, the blue or green roc gel of the present invention low dose group, the blue or green roc gel of the present invention high dose group mouse peritoneal oozes out amount of dye and model control group significant difference (P＜0.01 of having compared, P＜0.05), the blue or green roc ointment group of tradition, blue or green roc ointment 1,2,3 groups of mouse peritoneals ooze out amount of dye and the blue or green roc gel of the present invention high dose group is compared, it is worth lower slightly but there was no significant difference (P＞0.05), the blue or green roc gel of the present invention low dose group mouse peritoneal oozes out amount of dye and the blue or green roc gel of the present invention high dose group significant difference (P＜0.05) of having compared, in a word, the blue or green roc ointment group of tradition, 1 group of blue or green roc ointment, 2 groups of blue or green roc ointment, 3 groups of blue or green roc ointment, the blue or green roc gel of the present invention low dose group, the blue or green roc gel of the present invention high dose group all can suppress oozing out of mouse writhing and abdominal cavity dyestuff, have certain antiinflammatory and analgesic activity, its action effect is followed successively by the blue or green roc gel of the present invention high dose group＞3 groups＞traditional blue or green roc ointment group of 1 group＞blue or green roc ointment of 2 groups＞blue or green roc ointment of blue or green roc ointment, and wherein the blue or green roc gel of the present invention high dose group analgesic and anti-inflammatory effects effect better and have certain dose dependent.

Experimental example 6: the blue or green roc gel of the present invention causes the experimentation of rat pain whipping to thermostimulation

1, reagent, animal and instrument and reagent

(1) reagent

Blue or

green roc ointment

The blue or green roc gel (embodiment 30 is prepared from the description according to the present invention) of the present invention.

(2) animal

The SD rat, 100, male and female half and half, body weight 20 ± 2g, the college of traditional Chinese medicine provides by Gansu, the quality certification number: SCXK (sweet) 2008-013.

(3) instrument and reagent

Electronic balance, Haikang, Shanghai Electronic Instruments Plant; Stopwatch, Shenzhen epoch Powerise digital technology Co., Ltd; The water-bath of temperature-controlled, Changzhou Guohua Electric Appliance Co., Ltd., model: HH-501; Pipettor; Diclofenac potassium (positive control), Novartis Pharma AG, lot number: X0036.

2, method and result

(1) method

Choose that qualified (rat is immersed in the 54-55 ℃ of warm water to the time that afterbody gets rid of from tail point, claim TFL, claim the threshold of pain again, with pain threshold greater than 3 seconds, less than 6 seconds was qualified) 80 of healthy rats, be divided into 8 groups at random by body weight, be respectively: the I group is the blank group, II organizes positive matched group (diclofenac potassium 5mg/kg/d), the III group is traditional blue or green roc ointment group (0.48g (crude drug)/kg/d), the IV group is 1 group of (0.48g (crude drug)/kg/d) of blue or green roc ointment, the V group is 2 groups of (0.48g (crude drug)/kg/d) of blue or green roc ointment, (0.48g (crude drug)/kg/d), (0.12g (crude drug)/kg/d), the VIII group is the blue or green roc gel of the present invention high dose group (0.48g (crude drug)/kg/d) to the VII group to the VI group for the blue or green roc gel of the present invention low dose group for 3 groups of blue or green roc ointment.Measure the pain threshold of respectively organizing rat with stopwatch earlier before the administration, and keep a record, percutaneous dosing is six days continuously, 1h after the last administration, 2h, 4h, 6h measures respectively and respectively organizes the rat pain threshold, every time point determining three times is with mean value computation, if TFL surpasses 10 seconds, then stops test and calculates according to 10 seconds.After experiment finishes, add up and organize a t check, calculate threshold of pain raising percentage rate, compare analgesic effect between different dosage form medicine, the various dose, the results are shown in Table 13 and table 14 according to experimental result according to surveys pain threshold.

Percentage rate=(pain threshold after the administration of administration group-blank group pain threshold)/blank group pain threshold * 100% is improved in the threshold of pain

Table 13 gel of the present invention to thermostimulation cause the rat pain threshold influence (x ± s, n=10)

Annotate: each administration group and blank group compare: ¹P＜0.05, ²P＜0.01.

Table 14 gel of the present invention improves percentage rate (n=10) to the threshold of pain that thermostimulation causes rat

(2) statistical analysis

Each organizes rat pain threshold mean ± standard deviation

(3) result

Experimental result shows: respectively organize rat pain threshold and the blank group there was no significant difference of comparing before the drug treating, the blank group is at 1h, 2h, and 4h, the rat pain threshold slightly changes but there was no significant difference during 6h, as above shown in the table 14,15.Experimental result shows traditional blue or green roc ointment group, blue or green roc ointment 1,2,3 groups, the blue or green roc gel of the present invention low dose group, high dose group all has certain analgesic activity, pain threshold after its administration is with model matched group significant difference (P＜0.05 of having compared, P＜0.01), after the data show administration 2 hours the time analgesic activity best, the blue or green roc ointment group of tradition, blue or green roc ointment 1,2,3 groups, 2 hours threshold of pains of the blue or green roc gel of the present invention high dose group are improved percentage rate and are respectively 31.03%, 34.98%, 41.58%, 32.25% and 51.95%, its result shows traditional blue or green roc ointment group, 1 group of blue or green roc ointment, 2 groups of blue or green roc ointment, 3 groups of blue or green roc ointment, the analgesic effect of the blue or green roc gel of the present invention by by force to a little less than be followed successively by: the blue or green roc gel of the present invention, 2 groups of blue or green roc ointment, 1 group of blue or green roc ointment, 3 groups of blue or green roc ointment, the blue or green roc ointment group of tradition; The blue or green roc ointment group of tradition, 1,2,3 groups of blue or green roc ointment and the blue or green roc gel of the present invention high dose group 1h, 2h, 4h, the 6h pain threshold there was no significant difference (P＞0.05) of comparing, the blue or green roc gel of the present invention is low, each time point pain threshold of high dose group is compared there was no significant difference (P＞0.05), and each time point pain threshold of the blue or green roc gel of the present invention high dose group is higher; In a word, the better and one-tenth dose dependent of the blue or green roc gel of the present invention analgesic effect.

Experimental example 7: the blue or green roc gel of the present invention is to the experimentation of immune function of mice influence

1, reagent, animal and instrument and reagent

(1) reagent

Blue or

green roc ointment

The blue or green roc gel (embodiment 34 is prepared from the description according to the present invention) of the present invention.

(2) animal

BALB/C mice, male, body weight 20 ± 2g is provided by Beijing Vital River Experimental Animals Technology Co., Ltd..The quality certification number: SCXK (capital) 2006-0006.

(3) instrument and reagent

The XDS-1B inverted biological microscope, Chongqing Photoelectric Instruments Corp.; The LS-9800 liquid scintillation counter, U.S. BECKMAN company; Titertek Cell Harvester 550 bull cell harvestors, Britain; YAMATO IP-31 CO ₂Incubator, Japan; Cyclophosphamide, Hualian Pharmaceutical Co., Ltd., Shanghai, lot number 011216; ConA, LPS (lipopolysaccharide), RPMI1640 culture medium, U.S. Sigma company; Calf serum, U.S. Hyclone company; ³H-TdR, the atomic energy academy of the Chinese Academy of Sciences provides; 96 well culture plates are U.S. Costar company, trichloroacetic acid, dehydrated alcohol.

2, method and result

(1) method

2.1 gel of the present invention is to the LT influence of mouse spleen T

BALB/C mice, male 80, by 8 groups at random of body weight, be respectively: the I group is normal control group (substrate), II organizes positive matched group (cyclophosphamide 15mg/kg), the III group is traditional blue or green roc ointment group (0.60g (crude drug)/kg/d), the IV group is 1 group of (0.60g (crude drug)/kg/d) of blue or green roc ointment, the V group is 2 groups of (0.60g (crude drug)/kg/d) of blue or green roc ointment, the VI group is 3 groups of (0.60g (crude drug)/kg/d) of blue or green roc ointment, (0.15g (crude drug)/kg/d), the VIII group is the blue or green roc gel of the present invention high dose group (0.60g (crude drug)/kg/d) to the VII group for the blue or green roc gel of the present invention low dose group.I, III-VIII group is percutaneous dosing, every day 1 time, continuous 14 days.In administration the 12nd day, the equal ip cyclophosphamide of positive controls mice (cy) 15mg/kg, once a day for three days on end.After administration finished, each treated animal was all plucked the eyeball sacrificed by exsanguination, wins spleen under the aseptic condition, and with containing 10% calf serum RPMI-1640, weight/ml makes splenocyte suspension (5 * 10 by the 15mg spleen ⁶/ ml), cross 200 order steel wires, put in the ice bath stand-by.Precision takes by weighing LPS, is made into 1000 μ g/ml with 1640 liquid.Get the flat culture plate in 96 holes, every hole adds splenocyte suspension 100 μ L, and every spleen is made diplopore, and a hole does not add LPS, and a hole adds LPS 10 μ L, adds 10% calf serum RPMI-1640 again, and making every hole final volume is 200 μ L, shakes up, and puts 37 ℃, 5%CO ₂Incubator is cultivated 72h, 24h before stopping cultivating, and every hole adds ³H-TdR 3.7 * 10 ⁴Bq/20 μ L, cultivation finishes, and uses bull cell harvestor collecting cell on 49 flint glass F fibrous filter membranes, and uses distilled water, 5% trichloroacetic acid, dehydrated alcohol sucking filtration successively, filter membrane is put in the scintillation vial, the activity cpm value that participates in liquid flashing counting determining.With the cpm number that adds the LPS hole divided by the cpm number that does not add the LPS hole as LPS to the stimulation index that the spleen bone-marrow-derived lymphocyte transforms, the results are shown in Table 15.

2.2, gel of the present invention influence that the mouse spleen bone-marrow-derived lymphocyte is transformed

Mice group, administration, modeling type are the same.Precision takes by weighing ConA, and it is standby to be made into 100 μ g/ml with 1640 liquid.Get the flat culture plate in 96 holes, every hole adds splenocyte suspension (cell concentration is the same) 100 μ L, and every spleen is made diplopore, one hole does not add ConA, and a hole adds ConA 20 μ L, adds 10% calf serum RPMI-1640 again, making every hole final volume is 200 μ L, shakes up cell culture, adding ³H-TdR, collecting cell, mensuration cpm value method are the same, the results are shown in Table 16.

2.3, gel of the present invention is to the influence of mouse spleen, thymus index

Mice group, administration, modeling type are the same.Administration is plucked the eyeball sacrificed by exsanguination after finishing, and wins spleen fast, and thymus precision respectively takes by weighing its weight, calculates according to following formula, the results are shown in Table 17.

Index and spleen index=spleen weight (mg)/the weight of animals (g)

Thymus index=thymic weight (mg)/the weight of animals (g)

Table 15 gel of the present invention to the LT influence of mice spleen T (x ± s, n=10)

Annotate: each administration group and normal control group compare: ¹P＜0.05, ²P＜0.01; The blue or green roc ointment group of tradition, 1 group of blue or green roc ointment, 2 groups of blue or green roc ointment, 3 groups of blue or green roc ointment, the blue or green roc film-coated preparation of the present invention low dose group are compared with the blue or green roc film-coated preparation of the present invention high dose group respectively: ³P＜0.05, ⁴P＜0.01.

The influence that table 16 gel of the present invention transforms the mice spleen bone-marrow-derived lymphocyte (x ± s, n=10)

Annotate: each administration group and normal control group compare: ¹P＜0.05, ²P＜0.01; The blue or green roc ointment group of tradition, 1 group of blue or green roc ointment, 2 groups of blue or green roc ointment, 3 groups of blue or green roc ointment, the blue or green roc gel of the present invention low dose group are compared with the blue or green roc film-coated preparation of the present invention high dose group respectively: ³P＜0.05, ⁴P＜0.01.

Table 17 gel of the present invention to the influence of mice important organ coefficient (x ± s, n=10)

Annotate: each administration group and normal control group compare: ¹P＜0.05, ²P＜0.01, traditional blue or green roc ointment group, 1 group of blue or green roc ointment, 2 groups of blue or green roc ointment, 3 groups of blue or green roc ointment, the blue or green roc gel of the present invention low dose group are compared with the blue or green roc film-coated preparation of the present invention high dose group respectively: ³P＜0.05, ⁴P＜0.01.

(2) statistical analysis

Each organizes mice spleen T, bone-marrow-derived lymphocyte conversion, thymus index and spleen index all with mean ± standard deviation

(3) result

Experimental result such as table 15,16 and 17 show: traditional blue or green roc ointment group, blue or green roc ointment 1,2,3 groups, the blue or green roc gel of the present invention is low, high dose group all can reduce mice T, the conversion ratio of bone-marrow-derived lymphocyte, comparing with the normal control group all has significant difference (P＜0.05, P＜0.01, simultaneously with reference to adding ConA and not adding ConA), the blue or green roc gel of the present invention high dose group is reducing mice T, bone-marrow-derived lymphocyte conversion ratio aspect and traditional blue or green roc ointment group, blue or green roc ointment is compared for 3 groups all significant difference (simultaneously with reference to adding ConA and not adding ConA, P＜0.05, P＜0.01), the blue or green roc gel of the present invention is low, high dose group is reducing mice T, comparing in bone-marrow-derived lymphocyte conversion ratio aspect all has significant difference (simultaneously with reference to adding ConA and not adding ConA, P＜0.05, P＜0.01) and present dose dependent, the blue or green roc ointment group of tradition, blue or green roc ointment 1,2,3 groups, after the administration of the blue or green roc gel of the present invention low dose group, the mouse thymus index is lower than the normal control group but the there was no significant difference of comparing (P＞0.05), the blue or green roc gel of the present invention high dose group mouse thymus exponential sum normal control group relatively has significant difference (P＜0.05), blue or green roc ointment 1,2, blue or green roc gel high dose group mouse spleen index of 3 groups and the present invention and normal control group are compared significant difference (P＜0.01 are all arranged, P＜0.05), the blue or green roc gel high dose group of the present invention and traditional blue or green roc ointment group, the blue or green roc gel of the present invention low dose group mouse spleen index is compared significant difference (P＜0.01 is all arranged, P＜0.05), in a word, the blue or green roc ointment group of tradition, blue or green roc ointment 1,2,3 groups, the blue or green roc gel of the present invention is low, high dose group all can suppress mice T to a certain extent, the conversion of bone-marrow-derived lymphocyte, reduce model mice thymus, the organ coefficient of spleen, the result shows traditional blue or green roc ointment group, blue or green roc ointment 1,2,3 groups, the blue or green roc gel of the present invention is low, high dose group all has certain immunosuppressive action, and this may be relevant with blue or green roc preparation antiinflammatory mechanism.

Following embodiment all can realize the effect of above-mentioned experimental example.

The specific embodiment

Embodiment 1: the blue or green roc gel of the present invention

Crude drug is formed:

Herba Oxytropis Kansuensis 100g, sub-rhubarb extract 50g, Radix aconiti szechenyiani 75g, Fructus Chebulae (pitted) 100g, Fructus Terminaliae Billericae 100g, Fructus Phyllanthi 100g, Benzoinum 35g, Herba Lycopodii 150g, Moschus 25g;

Adjuvant consists of:

Carbomer 150g, triethanolamine 100g, Mentholum 62g, ethyl hydroxybenzoate 5g;

Preparation method:

1. Herba Oxytropis Kansuensis, sub-rhubarb extract, Radix aconiti szechenyiani, Fructus Chebulae (pitted), Fructus Terminaliae Billericae, Fructus Phyllanthi, Benzoinum, Herba Lycopodii are carried out remove impurity, cleaning, drying respectively, be ground into the superfine powder that particle diameter is 0.1-1 μ m;

2. Moschus is ground to form fine powder;

3. with the carbomer adding distil water, soaked overnight makes its abundant swelling, adds triethanolamine, Mentholum, ethyl hydroxybenzoate, stirs, and makes gel-type vehicle;

4. after the gel-type vehicle that 3. makes of the superfine powder that above-mentioned steps is made in 1., Moschus fine powder and step that 2. step obtains mixed, it was an amount of to add water, stirs, and leaves standstill, and packing promptly gets gel of the present invention.

Embodiment 2: the blue or green roc gel of the present invention

Crude drug is formed:

Adjuvant is formed:

Gelatin 350g, sodium carboxymethyl cellulose 280g, laurocapram 176g, glycerol 235g, butoben 12g;

Preparation method:

1. Herba Oxytropis Kansuensis, sub-rhubarb extract, Radix aconiti szechenyiani, Fructus Chebulae (pitted), Fructus Terminaliae Billericae, Fructus Phyllanthi, Benzoinum, Herba Lycopodii are carried out remove impurity, cleaning, drying respectively, be ground into the superfine powder that particle diameter is 1-10 μ m;

2. Moschus is ground to form fine powder;

3. with gelatin, the sodium carboxymethyl cellulose adding distil water, soaked overnight makes its abundant swelling, adds laurocapram, glycerol, ethyl hydroxybenzoate, stirs, and makes gel-type vehicle;

Embodiment 3: the blue or green roc gel of the present invention

Crude drug is formed:

Adjuvant is formed:

Poloxamer 407 120g, poloxamer 188 520g, ascorbic acid 80g, Polyethylene Glycol 150g, sodium benzoate 14.5g;

Preparation method:

1. Herba Oxytropis Kansuensis, sub-rhubarb extract, Radix aconiti szechenyiani, Fructus Chebulae (pitted), Fructus Terminaliae Billericae, Fructus Phyllanthi, Benzoinum, Herba Lycopodii are carried out remove impurity, cleaning, drying respectively, be ground into the superfine powder that particle diameter is 10-40 μ m;

2. Moschus is ground to form fine powder;

3. with poloxamer 407, poloxamer 188 adding distil waters, soaked overnight makes its abundant swelling, and ascorbic acid, Polyethylene Glycol, sodium benzoate stir, and make gel-type vehicle;

Embodiment 4: the blue or green roc gel of the present invention

Crude drug is formed:

Adjuvant is formed:

Poly-N N-isopropylacrylamide 280g, sodium hydrogen phosphate 30g, sodium thiosulfate 35g, Borneolum Syntheticum 37.5g, benzoic acid 4.5g;

Preparation method:

1. Herba Oxytropis Kansuensis, sub-rhubarb extract, Radix aconiti szechenyiani, Fructus Chebulae (pitted), Fructus Terminaliae Billericae, Fructus Phyllanthi, Benzoinum, Herba Lycopodii are carried out remove impurity, cleaning, drying respectively, be ground into the medicated powder that particle diameter is 40-60 μ m;

2. Moschus is ground to form fine powder;

3. will gather N N-isopropylacrylamide adding distil water, soaked overnight makes its abundant swelling, adds sodium hydrogen phosphate, sodium thiosulfate, Borneolum Syntheticum, benzoic acid, stirs, and makes gel-type vehicle;

4. after the gel-type vehicle that 3. makes of the medicated powder that above-mentioned steps is made in 1., Moschus fine powder and step that 2. step obtains mixed, it was an amount of to add water, stirs, and leaves standstill, and packing promptly gets gel of the present invention.

Embodiment 5: the blue or green roc gel of the present invention

Crude drug is formed:

Adjuvant is formed:

Remove acetyl-removed gellan gum 1530g, sodium hydroxide 560g, sodium pyrosulfite 320g, Decylmethyl Sulphoxide 445g, glycerol 525g, Polysorbate 145g, sorbic acid 25g;

Preparation method:

1. Herba Oxytropis Kansuensis, sub-rhubarb extract, Radix aconiti szechenyiani, Fructus Chebulae (pitted), Fructus Terminaliae Billericae, Fructus Phyllanthi, Benzoinum, Herba Lycopodii are carried out remove impurity, cleaning, drying respectively, be ground into the medicated powder that particle diameter is 60-90 μ m;

2. Moschus is ground to form fine powder;

3. will remove the acetyl-removed gellan gum adding distil water, soaked overnight makes its abundant swelling, adds sodium hydroxide, sodium pyrosulfite, Decylmethyl Sulphoxide, glycerol, Polysorbate, sorbic acid, stirs, and makes gel-type vehicle;

Embodiment 6: the blue or green roc gel of the present invention

Crude drug is formed:

Adjuvant is formed:

Methylcellulose 280g, ethyl cellulose 240g, sodium bicarbonate 152g, sodium sulfite 165g, methyl salicylate 212g, paraffin oil 300g, sodium lauryl sulphate 120g, phenethanol 9.5g;

Preparation method:

1. Herba Oxytropis Kansuensis, sub-rhubarb extract, Radix aconiti szechenyiani, Fructus Chebulae (pitted), Fructus Terminaliae Billericae, Fructus Phyllanthi, Benzoinum, Herba Lycopodii are carried out remove impurity, cleaning, drying respectively, be ground into the medicated powder that particle diameter is 90-150 μ m;

2. Moschus is ground to form fine powder;

3. with methylcellulose, ethyl cellulose adding distil water, soaked overnight makes its abundant swelling, adds sodium bicarbonate, sodium sulfite, methyl salicylate, paraffin oil, sodium lauryl sulphate, phenethanol, stirs, and makes gel-type vehicle;

Embodiment 7: the blue or green roc gel of the present invention

Crude drug is formed:

Adjuvant is formed:

Polyox-yethylene-polyoxypropylene block copolymer 120g, aluminium hydroxide 50g, sodium sulfite 39g, plant Oleum sesami 34g, propylparaben 4.5g;

Preparation method:

1. with Herba Oxytropis Kansuensis, sub-rhubarb extract, Radix aconiti szechenyiani, Fructus Chebulae (pitted), Fructus Terminaliae Billericae, Fructus Phyllanthi, Benzoinum, Herba Lycopodii remove impurity, clean system, mixing;

2. Moschus is ground to form fine powder;

5. with the polyox-yethylene-polyoxypropylene block copolymer adding distil water, soaked overnight makes its abundant swelling, adds aluminium hydroxide, sodium sulfite, plant Oleum sesami, propylparaben, stirs, and makes gel-type vehicle;

Embodiment 8: the blue or green roc gel of the present invention

Crude drug is formed:

Adjuvant is formed:

Sodium polyacrylate 180g, menthol 87g, mineral oil 50g, Polysorbate 45g, sodium benzoate 5g;

Preparation method:

2. Moschus is ground to form fine powder;

5. with the sodium polyacrylate adding distil water, soaked overnight makes its abundant swelling, adds menthol, mineral oil, Polysorbate, sodium benzoate, stirs, and makes gel-type vehicle;

6. after the gel-type vehicle that 5. the Moschus fine powder that 2. above-mentioned steps is obtained, the concentrated solution a that 3. step obtains, superfine powder and step that 4. step obtains make mixes, add water to the 300-600 weight portion, stir, leave standstill, packing promptly gets gel of the present invention.

Embodiment 9: the blue or green roc gel of the present invention

Crude drug is formed:

Herba Oxytropis Kansuensis 100g, sub-rhubarb extract 50g, Radix aconiti szechenyiani 75g, Fructus Chebulae (pitted) 100g, Fructus Terminaliae Billericae 100g, Fructus Phyllanthi 100g, Benzoinum 35g, Herba Lycopodii 150g, Moschus 25g, ethyl hydroxybenzoate 7.5g;

Adjuvant is formed:

Remove acetyl-removed gellan gum 165g, sodium alginate 205g, ammonia 47g, sodium sulfite 50g, Camphora 90g, squalane 120g, sorbic acid 10g;

Preparation method:

2. Moschus is ground to form fine powder;

4. will remain hybrid medicine and be ground into the medicated powder that particle diameter is 10-40 μ m;

5. will remove acetyl-removed gellan gum, sodium alginate adding distil water, soaked overnight makes its abundant swelling, adds ammonia, sodium sulfite, Camphora, squalane, sorbic acid, stirs, and makes gel-type vehicle;

6. after the gel-type vehicle that 5. the Moschus fine powder that 2. above-mentioned steps is obtained, the concentrated solution a that 3. step obtains, superfine powder and step that 4. step obtains make mixed, it was an amount of to add water, stirs, and leaves standstill, and packing promptly gets gel of the present invention.

Embodiment 10: the blue or green roc gel of the present invention

Crude drug is formed:

Adjuvant is formed:

Polyacrylic acid 274g, gelatin 256g, sodium citrate 118g, citric acid 72g, laurocapram 98g, glycerol 118g, sodium lauryl sulphate 54g, methyl hydroxybenzoate 10g;

Preparation method:

2. Moschus is ground to form fine powder;

4. will remain hybrid medicine and be ground into the medicated powder that particle diameter is 40-60 μ m;

5. with polyacrylic acid, gelatin adding distil water, soaked overnight makes its abundant swelling, adds sodium citrate, citric acid, laurocapram, glycerol, sodium lauryl sulphate, methyl hydroxybenzoate, stirs, and makes gel-type vehicle;

Embodiment 11: the blue or green roc gel of the present invention

Crude drug is formed:

Adjuvant is formed:

Polyox-yethylene-polyoxypropylene block copolymer 250g, Polyethylene Glycol based block copolymer 300g, sodium hydrogen phosphate 105g, tartaric acid 64g, Mentholum 50g, glycerol 168g, Polysorbate 25g, propylparaben 7.5g;

Preparation method:

2. Moschus is ground to form fine powder;

4. will remain hybrid medicine and be ground into the medicated powder that particle diameter is 60-90 μ m;

5. with polyox-yethylene-polyoxypropylene block copolymer, Polyethylene Glycol based block copolymer adding distil water, soaked overnight makes its abundant swelling, adds sodium hydrogen phosphate, tartaric acid, Mentholum, glycerol, Polysorbate, propylparaben, stir, make gel-type vehicle;

Embodiment 12: the blue or green roc gel of the present invention

Crude drug is formed:

Adjuvant is formed:

Polyox-yethylene-polyoxypropylene block copolymer 105g, sodium bicarbonate 25g, sodium pyrosulfite 37g, Decylmethyl Sulphoxide 42g, Polyethylene Glycol 48g, ethyl hydroxybenzoate 2.5g;

Preparation method:

2. Moschus is ground to form fine powder;

4. with the polyox-yethylene-polyoxypropylene block copolymer adding distil water, soaked overnight makes its abundant swelling, adds sodium bicarbonate, sodium pyrosulfite, Decylmethyl Sulphoxide, Polyethylene Glycol, ethyl hydroxybenzoate, stirs, and makes gel-type vehicle;

Embodiment 13: the blue or green roc gel of the present invention

Crude drug is formed:

Adjuvant is formed:

Gelatin 175g, chitosan 148g, ascorbic acid 58g, wax fat 150g, menthol 94g, benzoic acid 12g;

Preparation method:

2. Moschus is ground to form fine powder;

3. or take by weighing 10% of above-mentioned mixed material medicine gross weight, decocting with 6 times of weight boils 3 times, the each decoction 1 hour, collection, merge extractive liquid, and volatile oil filter, and the adsorption column of macroporous adsorbent resin is housed filtrate adding, with 15 times of amounts of 35% ethanol eluting, collect eluent, be condensed into the concentrated solution that relative density is 1.25-1.35, and with volatile oil join in the concentrated solution concentrated solution a;

5. with gelatin, chitosan adding distil water, soaked overnight makes its abundant swelling, adds ascorbic acid, wax fat, menthol, benzoic acid, stirs, and makes gel-type vehicle;

Embodiment 14: the blue or green roc gel of the present invention

Crude drug is formed:

Adjuvant is formed;

Methylcellulose 558g, ethyl cellulose 452g, ammonia 278g, sodium thiosulfate 154g, laurocapram 310g, Polyethylene Glycol 480g, sodium lauryl sulphate 225, phenethanol 20g;

Preparation method:

2. Moschus is ground to form fine powder;

3. take by weighing 20% of above-mentioned mixed material medicine gross weight, decocting with 8 times of weight boils 2 times, the each decoction 2 hours, collection, merge extractive liquid, and volatile oil filter, and the adsorption column of macroporous adsorbent resin is housed filtrate adding, with 20 times of amounts of 45% ethanol eluting, collect eluent, be condensed into the concentrated solution that relative density is 1.35-1.45, and with volatile oil join in the concentrated solution concentrated solution a;

5. with methylcellulose, ethyl cellulose adding distil water, soaked overnight makes its abundant swelling, adds ammonia, sodium thiosulfate, laurocapram, Polyethylene Glycol, sodium lauryl sulphate, phenethanol, stirs, and makes gel-type vehicle;

Embodiment 15: the blue or green roc gel of the present invention

Crude drug is formed:

Adjuvant is formed;

Polyacrylic acid 85g, sorbitol 18g, paraffin 54g, methyl salicylate 22g, sodium benzoate 4g;

Preparation method:

2. Moschus is ground to form fine powder;

3. take by weighing 50% of above-mentioned mixed material medicine gross weight, decocting with 10 times of weight boils 2 times, the each decoction 1.5 hours, collection, merge extractive liquid, and volatile oil filter, and the adsorption column of macroporous adsorbent resin is housed filtrate adding, with 25 times of amounts of 50% ethanol eluting, collect eluent, be condensed into the concentrated solution that relative density is 1.15-1.25, and with volatile oil join in the concentrated solution concentrated solution a;

5. with the polyacrylic acid adding distil water, soaked overnight makes its abundant swelling, adds sorbitol, paraffin, methyl salicylate, sodium benzoate, stirs, and makes gel-type vehicle;

Embodiment 16: the blue or green roc gel of the present invention

Crude drug is formed:

Adjuvant is formed:

Poly--2-ethoxy ethylene ether-poly-hydroxyethyl vinyl Ether block copolymer 285g, sodium hydroxide 112g, disodiumedetate 85g, Decylmethyl Sulphoxide 100g, wax fat 120g, sodium lauryl sulphate 64g, sorbic acid 5g;

Preparation method:

2. Moschus is ground to form fine powder;

3. take by weighing 60% of above-mentioned mixed material medicine gross weight, decocting with 12 times of weight boils 1 time, the each decoction 3 hours, collection, merge extractive liquid, and volatile oil filter, and the adsorption column of macroporous adsorbent resin is housed filtrate adding, with 30 times of amounts of 60% ethanol eluting, collect eluent, be condensed into the concentrated solution that relative density is 1.05-1.15, and with volatile oil join in the concentrated solution concentrated solution a;

5. will gather-2-ethoxy ethylene ether-poly-hydroxyethyl vinyl Ether block copolymer adding distil water, soaked overnight makes its abundant swelling, adds sodium hydroxide, disodiumedetate, Decylmethyl Sulphoxide, wax fat, sodium lauryl sulphate, sorbic acid, stir, make gel-type vehicle;

6. after the gel-type vehicle that 5. the Moschus fine powder that 2. above-mentioned steps is obtained, the concentrated solution a that 3. step obtains, medicated powder and step that 4. step obtains make mixed, it was an amount of to add water, stirs, and leaves standstill, and packing promptly gets gel of the present invention.

Embodiment 17: the blue or green roc gel of the present invention

Crude drug is formed:

Adjuvant is formed:

Poly-N N-isopropylacrylamide 750g, triethanolamine 338g, ethylenediaminetetraacetic acid 253g, glycerol 320g, paraffin 150g, Borneolum Syntheticum 210g, Polysorbate 184g, methyl hydroxybenzoate 25g;

Preparation method:

2. Moschus is ground to form fine powder;

3. take by weighing 90% of above-mentioned mixed material medicine gross weight, decocting with 15 times of weight boils 4 times, the each decoction 0.5 hour, collection, merge extractive liquid, and volatile oil filter, and the adsorption column of macroporous adsorbent resin is housed filtrate adding, with 35 times of amounts of 70% ethanol eluting, collect eluent, be condensed into the concentrated solution that relative density is 1.15-1.25, and with volatile oil join in the concentrated solution concentrated solution a;

5. will gather N N-isopropylacrylamide adding distil water, soaked overnight makes its abundant swelling, adds triethanolamine, ethylenediaminetetraacetic acid, glycerol, paraffin, Borneolum Syntheticum, Polysorbate, methyl hydroxybenzoate, stirs, and makes gel-type vehicle;

Embodiment 18: the blue or green roc gel of the present invention

Crude drug is formed:

Adjuvant is formed:

Carbomer 72g, mineral oil 18g, Mentholum 12g, ethyl hydroxybenzoate 2.5g;

Preparation method:

2. Moschus is ground to form fine powder;

3. take by weighing above-mentioned mixed material medicine, decocting with 10 times of weight boils 3 times, the each decoction 2 hours, collection, merge extractive liquid, and volatile oil filter, and the adsorption column of macroporous adsorbent resin is housed filtrate adding, with 40 times of amounts of 80% ethanol eluting, collect eluent, be condensed into the concentrated solution that relative density is 1.05-1.15, and with volatile oil join in the concentrated solution concentrated solution a;

4. with the carbomer adding distil water, soaked overnight makes its abundant swelling, adds mineral oil, Mentholum, ethyl hydroxybenzoate, stirs, and makes gel-type vehicle;

Embodiment 19: the blue or green roc gel of the present invention

Crude drug is formed:

Adjuvant is formed:

Sodium carboxymethyl cellulose 215g, sodium hydroxide 158g, sodium sulfite 90g, menthol 136g, propylene glycol 188g, Polysorbate 87g, phenethanol 4.5g;

Preparation method:

2. Moschus is ground to form fine powder;

3. take by weighing 10% of above-mentioned mixed material medicine gross weight, with 40% alcohol reflux of 6 times of weight 3 times, extracted 1 hour at every turn, collect, merge extractive liquid,, filter, being condensed into relative density is the concentrated solution a of 1.25-1.35;

5. with the sodium carboxymethyl cellulose adding distil water, soaked overnight makes its abundant swelling, adds sodium hydroxide, sodium sulfite, menthol, propylene glycol, Polysorbate, phenethanol, stirs, and makes gel-type vehicle;

Embodiment 20: the blue or green roc gel of the present invention

Crude drug is formed:

Adjuvant is formed:

Gelatin 100g, sodium citrate 75g, laurocapram 50g;

Preparation method:

2. Moschus is ground to form fine powder;

3. take by weighing 20% of above-mentioned mixed material medicine gross weight, with 50% alcohol reflux of 8 times of weight 2 times, extracted 2 hours at every turn, collect, merge extractive liquid,, filter, being condensed into relative density is the concentrated solution a of 1.35-1.45;

5. with the gelatin adding distil water, soaked overnight makes its abundant swelling, adds sodium citrate, laurocapram, stirs, and makes gel-type vehicle;

Embodiment 21: the blue or green roc gel of the present invention

Crude drug is formed:

Adjuvant is formed:

Poly-N N-isopropylacrylamide 484g, ammonia 80g, sodium sulfite 66g, Decylmethyl Sulphoxide 67g, silicone oil aliphatic alcohol 105g, Polysorbate 72g, sorbic acid 9.5g;

Preparation method:

2. Moschus is ground to form fine powder;

3. take by weighing 50% of above-mentioned mixed material medicine gross weight, with 60% alcohol reflux of 10 times of weight 2 times, extracted 1.5 hours at every turn, collect extracting solution, filter, being condensed into relative density is the concentrated solution a of 1.15-1.25;

5. will gather N N-isopropylacrylamide adding distil water, soaked overnight makes its abundant swelling, adds ammonia, sodium sulfite, Decylmethyl Sulphoxide, silicone oil aliphatic alcohol, Polysorbate, sorbic acid, stirs, and makes gel-type vehicle;

Embodiment 22: the blue or green roc gel of the present invention

Crude drug is formed:

Adjuvant is formed:

Poly--2-ethoxy ethylene ether-poly-hydroxyethyl vinyl Ether block copolymer 50g, sodium bicarbonate 30g, disodiumedetate 16g, plant Oleum sesami 46g, propylene glycol 58g, sodium lauryl sulphate 10g;

Preparation method:

2. Moschus is ground to form fine powder;

3. take by weighing 60% of above-mentioned mixed material medicine gross weight, with 75% alcohol reflux of 12 times of weight 1 time, extracted 24 hours, collect, merge extractive liquid,, filter, being condensed into relative density is the concentrated solution a of 1.05-1.15;

5. will gather-2-ethoxy ethylene ether-poly-hydroxyethyl vinyl Ether block copolymer adding distil water, soaked overnight makes its abundant swelling, adds sodium bicarbonate, disodiumedetate, plant Oleum sesami, propylene glycol, sodium lauryl sulphate, stir, make gel-type vehicle;

Embodiment 23: the blue or green roc gel of the present invention

Crude drug is formed:

Adjuvant is formed:

Remove acetyl-removed gellan gum 265g, chitosan 385g, aluminium hydroxide 130g, tartaric acid 78g, Borneolum Syntheticum 108g, sorbitol 174g, sodium lauryl sulphate 66g, butoben 12g;

Preparation method:

2. Moschus is ground to form fine powder;

3. take by weighing 90% of above-mentioned mixed material medicine gross weight, with 80% alcohol reflux of 15 times of weight 4 times, extracted 0.5 hour at every turn, collect extracting solution, filter, being condensed into relative density is the concentrated solution a of 1.15-1.25;

5. will remove acetyl-removed gellan gum, chitosan adding distil water, soaked overnight makes its abundant swelling, adds aluminium hydroxide, tartaric acid, Borneolum Syntheticum, sorbitol, sodium lauryl sulphate, butoben, stirs, and makes gel-type vehicle;

Embodiment 24: the blue or green roc gel of the present invention

Crude drug is formed:

Adjuvant is formed:

Carbomer 220g, triethanolamine 33g, Camphora 18g, Borneolum Syntheticum 20g, silicone oil aliphatic alcohol 46g, polysorbate 20 g, ethyl hydroxybenzoate 5g;

Preparation method:

2. Moschus is ground to form fine powder;

3. take by weighing above-mentioned mixed material medicine, use 60% alcohol reflux 3 times of 10,8,6 times of medicine gross weights respectively, extracted respectively 90,60,30 minutes at every turn, collect, merge extractive liquid,, filter, being condensed into relative density is the concentrated solution a of 1.05-1.15;

4. with the carbomer adding distil water, soaked overnight makes its abundant swelling, adds triethanolamine, Camphora, Borneolum Syntheticum, silicone oil aliphatic alcohol, Polysorbate, ethyl hydroxybenzoate, stirs, and makes gel-type vehicle;

Embodiment 25: the blue or green roc gel of the present invention

Crude drug is formed:

Adjuvant is formed:

Sodium alginate 85g, sodium polyacrylate 268g, sodium hydroxide 180g, disodiumedetate 100g, methyl salicylate 150g, sorbic acid 5g;

Preparation method:

2. Moschus is ground to form fine powder;

3. take by weighing 10% of above-mentioned mixed material medicine gross weight, 40% ethanol that adds medicated powder weight 0.8 times of weight stirs and soaked into 1 hour, in the percolation jar of packing into, ethanol percolation with 6 times of weight 40%, collect, merge percolate, filter, being evaporated to relative density is the concentrated solution a of 1.25-1.35;

5. with sodium alginate, sodium polyacrylate adding distil water, soaked overnight makes its abundant swelling, adds sodium hydroxide, disodiumedetate, methyl salicylate, sorbic acid, stirs, and makes gel-type vehicle;

Embodiment 26: the blue or green roc gel of the present invention

Crude drug is formed:

Herba Oxytropis Kansuensis 100g, sub-rhubarb extract 50g, Radix aconiti szechenyiani 75g, Fructus Chebulae (pitted) 100g, Fructus Terminaliae Billericae 100g, Fructus Phyllanthi 100g, Benzoinum 35g, Herba Lycopodii 150g, Moschus 25g

Adjuvant is formed:

Poloxamer 407 360g, poloxamer 188 85g, laurocapram 52g;

Preparation method:

2. Moschus is ground to form fine powder;

3. take by weighing 20% of above-mentioned mixed material medicine gross weight, 50% ethanol that adds medicated powder weight 0.9 times of weight stirs and soaked into 1.5 hours, in the percolation jar of packing into, ethanol percolation with 8 times of weight 50%, collect, merge percolate, filter, being evaporated to relative density is the concentrated solution a of 1.0-1.5;

5. with poloxamer 407, poloxamer 188 adding distil waters, soaked overnight makes its abundant swelling, adds laurocapram, stirs, and makes gel-type vehicle;

Embodiment 27: the blue or green roc gel of the present invention

Crude drug consists of:

Adjuvant is formed:

Chitosan 285g, sodium bicarbonate 126g, citric acid 88g, Decylmethyl Sulphoxide 158g, Polyethylene Glycol 180g, ethyl hydroxybenzoate 15g;

Preparation method:

2. Moschus is ground to form fine powder;

3. take by weighing 50% of above-mentioned mixed material medicine gross weight, 60% ethanol that adds medicated powder weight 1.0 times of weight stirs and soaked into 0.5 hour, pack in the percolation jar, ethanol percolation with 10 times of weight 60%, collect, merge percolate, filter, being evaporated to relative density is the concentrated solution a of 1.15-1.25;

5. with the chitosan adding distil water, soaked overnight makes its abundant swelling, adds sodium bicarbonate, citric acid, Decylmethyl Sulphoxide, Polyethylene Glycol, ethyl hydroxybenzoate, stirs, and makes gel-type vehicle;

Embodiment 28: the blue or green roc gel of the present invention

Crude drug is formed:

Adjuvant is formed:

Sodium polyacrylate 118g, sodium hydrogen phosphate 60g, sodium sulfite 45g, sodium lauryl sulphate 28g;

Preparation method:

2. Moschus is ground to form fine powder;

3. take by weighing 60% of above-mentioned mixed material medicine gross weight, 75% ethanol that adds medicated powder weight 0.8 times of weight stirs and soaked into 1 hour, in the percolation jar of packing into, ethanol percolation with 12 times of weight 75%, collect, merge percolate, filter, being evaporated to relative density is the concentrated solution a of 1.05-1.15;

5. with the sodium polyacrylate adding distil water, soaked overnight makes its abundant swelling, adds sodium hydrogen phosphate, sodium sulfite, sodium lauryl sulphate, stirs, and makes gel-type vehicle;

Embodiment 29: the blue or green roc gel of the present invention

Crude drug is formed:

Adjuvant is formed:

Poly--2-ethoxy ethylene ether-poly-hydroxyethyl vinyl Ether block copolymer 180g, polyox-yethylene-polyoxypropylene block copolymer 65, Polyethylene Glycol based block copolymer 120g, sodium hydrogen phosphate 150g, plant Oleum sesami 130g, paraffin oil 174g, sodium benzoate 10g;

Preparation method:

2. Moschus is ground to form fine powder;

3. take by weighing 90% of above-mentioned mixed material medicine gross weight, 80% ethanol that adds medicated powder weight 0.6 times of weight stirs and soaked into 5 hours, in the percolation jar of packing into, 80% ethanol percolation with 15 times of weight, collect, merge percolate, filter, being evaporated to relative density is the concentrated solution a of 1.15-1.25;

5. will gather-2-ethoxy ethylene ether-poly-hydroxyethyl vinyl Ether block copolymer, polyox-yethylene-polyoxypropylene block copolymer, Polyethylene Glycol based block copolymer adding distil water, soaked overnight, make its abundant swelling, add sodium hydrogen phosphate, plant Oleum sesami, paraffin oil, sodium benzoate, stir, make gel-type vehicle;

Embodiment 30: the blue or green roc gel of the present invention

Crude drug is formed:

Adjuvant is formed:

Sodium carboxymethyl cellulose 72g, sodium citrate 36g, ascorbic acid 24g, Decylmethyl Sulphoxide 40g, glycerol 66g, Polysorbate 18g, sorbic acid 7.5g;

Preparation method:

2. Moschus is ground to form fine powder;

3. take by weighing above-mentioned mixed material medicine, 60% ethanol that adds medicated powder weight 1.0 times of weight stirs and soaked into 1 hour, in the percolation jar of packing into, 60% ethanol percolation with 10 times of weight, collect, merge percolate, filter, being evaporated to relative density is the concentrated solution a of 1.05-1.15;

4. with the sodium carboxymethyl cellulose adding distil water, soaked overnight makes its abundant swelling, adds sodium citrate, ascorbic acid, Decylmethyl Sulphoxide, glycerol, Polysorbate, sorbic acid, stirs, and makes gel-type vehicle;

Embodiment 31: the blue or green roc gel of the present invention

Crude drug is formed:

Adjuvant is formed:

Sodium alginate 285g, triethanolamine 136g, ethylenediaminetetraacetic acid 45g, Mentholum 102g, propylene glycol 200g, sodium lauryl sulphate 50g;

Preparation method:

2. Moschus is ground to form fine powder;

3. take by weighing 10% of above-mentioned mixed material medicine gross weight, extract 3 times with 40% alcohol dipping of 6 times of weight, flooded 16 hours at every turn, collect, merge impregnation liquid, filtering and be evaporated to relative density is the concentrated solution a of 1.25-1.35;

5. with the sodium alginate adding distil water, soaked overnight makes its abundant swelling, adds triethanolamine, ethylenediaminetetraacetic acid, Mentholum, propylene glycol, sodium lauryl sulphate, stirs, and makes gel-type vehicle;

Embodiment 32: the blue or green roc gel of the present invention

Crude drug is formed:

Adjuvant is formed:

Carbomer 700g, gelatin 950g, sodium hydroxide 528g, citric acid 404g, Camphora 250g, Borneolum Syntheticum 150g, glycerol 420g, propylene glycol 230g, sodium lauryl sulphate 180g, phenethanol 25g;

Preparation method:

2. Moschus is ground to form fine powder;

3. take by weighing 20% of above-mentioned mixed material medicine gross weight, extract 2 times with 50% alcohol dipping of 8 times of weight, flooded 18 hours at every turn, collect, merge impregnation liquid, filtering and be evaporated to relative density is the concentrated solution a of 1.35-1.45;

5. with carbomer, gelatin adding distil water, soaked overnight makes its abundant swelling, adds sodium hydroxide, citric acid, Camphora, Borneolum Syntheticum, glycerol, propylene glycol, sodium lauryl sulphate, phenethanol, stirs, and makes gel-type vehicle;

Embodiment 33: the blue or green roc gel of the present invention

Crude drug is formed:

Adjuvant is formed:

Methylcellulose 120g, ethyl cellulose 165g, aluminium hydroxide 90g, sodium sulfite 136g, laurocapram 180g, Polyethylene Glycol 203g, Polysorbate 57g, methyl hydroxybenzoate 5g;

Preparation method:

2. Moschus is ground to form fine powder;

3. take by weighing 50% of above-mentioned mixed material medicine gross weight, extract 2 times with 60% alcohol dipping of 10 times of weight, flooded 20 hours at every turn, collect, merge impregnation liquid, filtering and be evaporated to relative density is the concentrated solution a of 1.15-1.25;

5. with methylcellulose, ethyl cellulose adding distil water, soaked overnight makes its abundant swelling, adds aluminium hydroxide, sodium sulfite, laurocapram, Polyethylene Glycol, Polysorbate, methyl hydroxybenzoate, stirs, and makes gel-type vehicle;

Embodiment 34: the blue or green roc gel of the present invention

Crude drug is formed:

Adjuvant is formed:

Polyethylene Glycol based block copolymer 65g, Decylmethyl Sulphoxide 54g, squalane 39g;

Preparation method:

2. Moschus is ground to form fine powder;

3. take by weighing 60% of above-mentioned mixed material medicine gross weight, extract 1 time with 75% alcohol dipping of 12 times of weight, flooded 24 hours, collect, merge impregnation liquid, filtering and be evaporated to relative density is the concentrated solution a of 1.05-1.15;

4. will remain hybrid medicine and be ground into the superfine powder that particle diameter is 40-60 μ m;

5. with Polyethylene Glycol based block copolymer adding distil water, soaked overnight makes its abundant swelling, adds Decylmethyl Sulphoxide, squalane, stirs, and makes gel-type vehicle;

Embodiment 35: the blue or green roc gel of the present invention

Crude drug is formed:

Adjuvant is formed:

Polyacrylic acid 388g, Mentholum 54g;

Preparation method:

2. Moschus is ground to form fine powder;

3. take by weighing 90% of above-mentioned mixed material medicine gross weight, extract 4 times with 80% alcohol dipping of 15 times of weight, flooded 12 hours at every turn, collect, merge impregnation liquid, filtering and be evaporated to relative density is the concentrated solution a of 1.15-1.25;

4. will remain hybrid medicine and be ground into the superfine powder that particle diameter is 60-90 μ m;

5. with the polyacrylic acid adding distil water, soaked overnight makes its abundant swelling, adds Mentholum, stirs, and makes gel-type vehicle;

Embodiment 36: the blue or green roc gel of the present invention

Crude drug is formed:

Adjuvant is formed:

Poloxamer 407 305g, poloxamer 188 198g, triethanolamine 28g, tartaric acid 30g, laurocapram 46g, sodium benzoate 3.5g;

Preparation method:

2. Moschus is ground to form fine powder;

3. take by weighing above-mentioned mixed material medicine, extract 3 times with 60% alcohol dipping of 10 times of weight, flooded 18 hours at every turn, collect, merge impregnation liquid, filtering and be evaporated to relative density is the concentrated solution a of 1.05-1.15;

4. with poloxamer 407, poloxamer 188 adding distil waters, soaked overnight makes its abundant swelling, adds triethanolamine, tartaric acid, laurocapram, sodium benzoate, stirs, and makes gel-type vehicle;

More than the description of better embodiment of the present invention is not limited the present invention, those skilled in the art can make various changes or distortion according to the present invention, only otherwise break away from spirit of the present invention, all should belong to the scope of claims of the present invention.

Claims

1. a gel that is used for reducing swelling and alleviating pain comprises active component and gel-type vehicle, wherein,

Described gel-type vehicle comprises the adjuvant of following proportioning:

Gel skeleton material 40～1600 weight portions.

2. gel according to claim 1, wherein, described gel-type vehicle further comprises the transdermal enhancer of 0.01-450 weight portion;

Described transdermal enhancer is to be selected from Borneolum Syntheticum, Camphora, Mentholum, menthol, methyl salicylate, laurocapram, Decylmethyl Sulphoxide and the plant Oleum sesami one or more;

Described gel skeleton material is to be selected from carbomer, sodium alginate, poloxamer, polyox-yethylene-polyoxypropylene block copolymer, Polyethylene Glycol based block copolymer, poly--2-ethoxy ethylene ether-poly-hydroxyethyl vinyl Ether block copolymer, poly-N N-isopropylacrylamide, polyacrylic acid, sodium polyacrylate, gelatin, methylcellulose, ethyl cellulose, sodium carboxymethyl cellulose, chitosan, to remove in the acetyl-removed gellan gum one or more.

3. gel according to claim 1 and 2, wherein, described gel-type vehicle further comprises the wetting agent of 0.01-550 weight portion;

Described wetting agent is to be selected from glycerol, Polyethylene Glycol, sorbitol, propylene glycol, paraffin oil, mineral oil, paraffin, squalane, silicone oil aliphatic alcohol and the wax fat one or more.

4. according to claim 1,2 or 3 described gels, wherein, described gel-type vehicle further comprises one or more adjuvants in the antibacterial of the surfactant of stabilizing agent, 0.01-250 weight portion of the pH regulator agent that is selected from the 0.01-600 weight portion, 0.01-350 weight portion and 0.01-30 weight portion;

Described pH regulator agent is to be selected from ammonia, triethanolamine, sodium hydroxide, aluminium hydroxide, sodium bicarbonate, sodium hydrogen phosphate and the sodium citrate one or more;

Described stabilizing agent is to be selected from sodium pyrosulfite, sodium sulfite, sodium sulfite, sodium thiosulfate, ascorbic acid, disodiumedetate, ethylenediaminetetraacetic acid, citric acid and the tartaric acid one or more;

Described surfactant is to be selected from Polysorbate and the sodium lauryl sulphate one or both; And

Described antibacterial is to be selected from parabens, phenethanol, sodium benzoate and the sorbic acid one or more.

5. according to the described gel of one of claim 1-4, wherein, in described active component:

Moschus is made the fine powder that particle diameter is 1-180 μ m.

6. according to the described gel of one of claim 1-5, wherein, described active component is made by following raw materials in weight portion medicine:

7. according to the described gel of one of claim 2-6, wherein,

Described gel skeleton material is for being selected from carbomer 50-750 weight portion, sodium alginate 50-300 weight portion, poloxamer 50-550 weight portion, polyox-yethylene-polyoxypropylene block copolymer 80-280 weight portion, Polyethylene Glycol based block copolymer 50-400 weight portion, poly--2-ethoxy ethylene ether-poly-hydroxyethyl vinyl Ether block copolymer 40-300 weight portion, poly-N N-isopropylacrylamide 250-800 weight portion, polyacrylic acid 50-400 weight portion, sodium polyacrylate 100-300 weight portion, gelatin 80-1000 weight portion, methylcellulose 100-600 weight portion, ethyl cellulose 150-500 weight portion, sodium carboxymethyl cellulose 50-300 weight portion, chitosan 120-400 weight portion, remove in the acetyl-removed gellan gum 150-1600 weight portion one or more;

Described transdermal enhancer is to be selected from Borneolum Syntheticum 0.01-220 weight portion, Camphora 0.01-260 weight portion, Mentholum 0.01-120 weight portion, menthol 0.01-150 weight portion, methyl salicylate 0.01-230 weight portion, laurocapram 0.01-200 weight portion, Decylmethyl Sulphoxide 0.01-450 weight portion, the plant Oleum sesami 0.01-140 weight portion one or more.

8. according to the described gel of one of claim 3-7, wherein,

Described wetting agent is to be selected from glycerol 0.01-550 weight portion, Polyethylene Glycol 0.01-500 weight portion, sorbitol 0.01-180 weight portion, propylene glycol 0.01-250 weight portion, paraffin oil 0.01-20 weight portion, mineral oil 0.01-65 weight portion, paraffin 0.01-165 weight portion, squalane 0.01-130 weight portion, silicone oil aliphatic alcohol 0.01-120 weight portion and the wax fat 0.01-165 weight portion one or more.

9. according to the described gel of one of claim 4-8, wherein,

Described PH regulator is to be selected from ammonia 0.01-300 weight portion, triethanolamine 0.01-350 weight portion, sodium hydroxide 0.01-600 weight portion, aluminium hydroxide 0.01-150 weight portion, sodium bicarbonate 0.01-180 weight portion, sodium hydrogen phosphate 0.01-180 weight portion and the sodium citrate 0.01-150 parts by volume one or more;

Described stabilizing agent is to be selected from sodium pyrosulfite 0.01-350 weight portion, sodium sulfite 0.01-120 weight portion, sodium sulfite 0.01-180 weight portion, sodium thiosulfate 0.01-160 weight portion, ascorbic acid 0.01-90 weight portion, disodiumedetate 0.01-120 weight portion, ethylenediaminetetraacetic acid 0.01-260 weight portion, citric acid 0.01-410 weight portion and the tartaric acid 0.01-80 weight portion one or more;

Described surfactant is to be selected from Polysorbate 0.01-200 weight portion and the sodium lauryl sulphate 0.01-250 weight portion one or both;

Described antibacterial is to be selected from parabens 0.01-30 weight portion, phenethanol 0.01-30 weight portion, sodium benzoate 0.01-15 weight portion and the sorbic acid 0.01-30 weight portion one or more.

10. gel according to claim 9, wherein,

Described gel skeleton material is for being selected from carbomer 150-600 weight portion, sodium alginate 100-200 weight portion, poloxamer 150-450 weight portion, polyox-yethylene-polyoxypropylene block copolymer 150-200 weight portion, Polyethylene Glycol based block copolymer 150-300 weight portion, poly--2-ethoxy ethylene ether-poly-hydroxyethyl vinyl Ether block copolymer 100-200 weight portion, poly-N N-isopropylacrylamide 400-600 weight portion, polyacrylic acid 150-300 weight portion, sodium polyacrylate 150-250 weight portion, gelatin 300-800 weight portion, methylcellulose 200-400 weight portion, ethyl cellulose 250-400 weight portion, sodium carboxymethyl cellulose 100-250 weight portion, chitosan 200-300 weight portion, remove in the acetyl-removed gellan gum 500-1000 weight portion one or more;

Described PH regulator is to be selected from ammonia 100-200 weight portion, triethanolamine 100-250 weight portion, sodium hydroxide 200-400 weight portion, aluminium hydroxide 50-100 weight portion, sodium bicarbonate 50-100 weight portion, sodium hydrogen phosphate 50-100 weight portion and the sodium citrate 50-100 parts by volume one or more;

Described stabilizing agent is to be selected from sodium pyrosulfite 100-250 weight portion, sodium sulfite 30-90 weight portion, sodium sulfite 50-100 weight portion, sodium thiosulfate 50-100 weight portion, ascorbic acid 30-60 weight portion, disodiumedetate 30-90 weight portion, ethylenediaminetetraacetic acid 100-150 weight portion, citric acid 100-300 weight portion and the tartaric acid 20-60 weight portion one or more;

Described transdermal enhancer is to be selected from Borneolum Syntheticum 0.01-220 weight portion, Camphora 0.01-260 weight portion, Mentholum 0.01-120 weight portion, menthol 0.01-150 weight portion, methyl salicylate 0.01-230 weight portion, laurocapram 0.01-200 weight portion, Decylmethyl Sulphoxide 0.01-450 weight portion and the plant Oleum sesami 0.01-140 weight portion one or more;

Described wetting agent is to be selected from glycerol 150-400 weight portion, Polyethylene Glycol 150-350 weight portion, sorbitol 50-100 weight portion, propylene glycol 50-150 weight portion, paraffin oil 150-250 weight portion, mineral oil 10-50 weight portion, paraffin 50-100 weight portion, squalane 30-100 weight portion, silicone oil aliphatic alcohol 20-100 weight portion and the wax fat 50-100 weight portion one or more

Described surfactant is to be selected from Polysorbate 50-150 weight portion and the sodium lauryl sulphate 50-200 weight portion one or both;

Described antibacterial is to be selected from parabens 5-25 weight portion, phenethanol 5-25 weight portion, sodium benzoate 5-10 weight portion and the sorbic acid 5-25 weight portion one or more.

11. according to claim 9 or 10 described gels, wherein,

Described gel skeleton material is for being selected from carbomer 400 weight portions, sodium alginate 175 weight portions, husky 300 weight portions in pool Lip river, polyox-yethylene-polyoxypropylene block copolymer 180 weight portions, Polyethylene Glycol based block copolymer 225 weight portions, poly--2-ethoxy ethylene ether-poly-hydroxyethyl vinyl Ether block copolymer 170 weight portions, poly-N N-isopropylacrylamide 525 weight portions, polyacrylic acid 225 weight portions, sodium polyacrylate 200 weight portions, gelatin 540 weight portions, methylcellulose 350 weight portions, ethyl cellulose 325 weight portions, sodium carboxymethyl cellulose 175 weight portions, chitosan 260 weight portions, remove in acetyl-removed gellan gum 875 weight portions one or more;

Described PH regulator is to be selected from ammonia 150 weight portions, triethanolamine 175 weight portions, sodium hydroxide 300 weight portions, aluminium hydroxide 75 weight portions, sodium bicarbonate 90 weight portions, sodium hydrogen phosphate 90 weight portions and sodium citrate 75 parts by volume one or more;

Described stabilizing agent is for being selected from sodium pyrosulfite 175 weight portions, sodium sulfite 60 weight portions, sodium sulfite 90 weight portions, sodium thiosulfate 80 weight portions, ascorbic acid 45 weight portions, disodiumedetate (EDTA-2Na) 60 weight portions, ethylenediaminetetraacetic acid 130 weight portions, citric acid 205 weight portions and tartaric acid 40 weight portions one or more;

Described transdermal enhancer is to be selected from Borneolum Syntheticum 110 weight portions, Camphora 130 weight portions, Mentholum 60 weight portions, menthol 75 weight portions, methyl salicylate 115 weight portions, laurocapram 100 weight portions, Decylmethyl Sulphoxide 225 weight portions and plant Oleum sesami 70 weight portions one or more;

Described wetting agent is to be selected from glycerol 275 weight portions, Polyethylene Glycol 250 weight portions, sorbitol 90 weight portions, propylene glycol 125 weight portions, paraffin oil 160 weight portions, mineral oil 32 weight portions, paraffin 83 weight portions, squalane 65 weight portions, silicone oil aliphatic alcohol 60 weight portions and wax fat 82 weight portions one or more;

Described surfactant is to be selected from Polysorbate 100 weight portions and sodium lauryl sulphate 125 weight portions one or both;

Described antibacterial is to be selected from parabens 15 weight portions, phenethanol 15 weight portions, sodium benzoate 7 weight portions and sorbic acid 15 weight portions one or more.

12. according to claim 9 or 10 described gels, wherein,

Described gel skeleton material is for being selected from carbomer 700 weight portions, sodium alginate 55 weight portions, poloxamer 500 weight portions, polyox-yethylene-polyoxypropylene block copolymer 85 weight portions, Polyethylene Glycol based block copolymer 390 weight portions, poly--2-ethoxy ethylene ether-poly-hydroxyethyl vinyl Ether block copolymer 45 weight portions, poly-N N-isopropylacrylamide 750 weight portions, polyacrylic acid 55 weight portions, sodium polyacrylate 295 weight portions, gelatin 85 weight portions, methylcellulose 550 weight portions, ethyl cellulose 155 weight portions, sodium carboxymethyl cellulose 295 weight portions, chitosan 125 weight portions, remove in acetyl-removed gellan gum 1500 weight portions one or more;

Described PH regulator is to be selected from ammonia 10 weight portions, triethanolamine 340 weight portions, sodium hydroxide 50 weight portions, aluminium hydroxide 145 weight portions, sodium bicarbonate 10 weight portions, sodium hydrogen phosphate 175 weight portions and sodium citrate 10 parts by volume one or more;

Described stabilizing agent is for being selected from sodium pyrosulfite 345 weight portions, sodium sulfite 10 weight portions, sodium sulfite 175 weight portions, sodium thiosulfate 10 weight portions, ascorbic acid 85 weight portions, disodiumedetate (EDTA-2Na) 10 weight portions, ethylenediaminetetraacetic acid 255 weight portions, citric acid 50 weight portions and tartaric acid 75 weight portions one or more;

Described transdermal enhancer is to be selected from Borneolum Syntheticum 10 weight portions, Camphora 255 weight portions, Mentholum 10 weight portions, menthol 145 weight portions, methyl salicylate 20 weight portions, laurocapram 195 weight portions, Decylmethyl Sulphoxide 50 weight portions, plant Oleum sesami 135 weight portions one or more;

Described wetting agent is to be selected from glycerol 500 weight portions, Polyethylene Glycol 50 weight portions, sorbitol 175 weight portions, propylene glycol 10 weight portions, paraffin oil 310 weight portions, mineral oil 10 weight portions, paraffin 160 weight portions, squalane 10 weight portions, silicone oil aliphatic alcohol 115 weight portions and wax fat 10 weight portions one or more;

Described surfactant is to be selected from Polysorbate 10 weight portions and sodium lauryl sulphate 245 weight portions one or more;

Described antibacterial is to be selected from parabens 25 weight portions, phenethanol 5 weight portions, sodium benzoate 10 weight portions and sorbic acid 5 weight portions one or more.

13. according to claim 9 or 10 described gels, wherein,

Described gel skeleton material is for being selected from carbomer 55 weight portions, sodium alginate 295 weight portions, poloxamer 55 weight portions, polyox-yethylene-polyoxypropylene block copolymer 275 weight portions, Polyethylene Glycol based block copolymer 55 weight portions, poly--2-ethoxy ethylene ether-poly-hydroxyethyl vinyl Ether block copolymer 295 weight portions, poly-N N-isopropylacrylamide 260 weight portions, polyacrylic acid 395 weight portions, sodium polyacrylate 110 weight portions, gelatin 950 weight portions, methylcellulose 110 weight portions, ethyl cellulose 495 weight portions, sodium carboxymethyl cellulose 55 weight portions, chitosan 395 weight portions, remove in acetyl-removed gellan gum 160 weight portions one or more;

Described PH regulator is to be selected from ammonia 295 weight portions, triethanolamine 20 weight portions, sodium hydroxide 590 weight portions, aluminium hydroxide 10 weight portions, sodium bicarbonate 175 weight portions, sodium hydrogen phosphate 10 weight portions and sodium citrate 145 parts by volume one or more;

Described stabilizing agent is to be selected from sodium pyrosulfite 20 weight portions, sodium sulfite 115 weight portions, sodium sulfite 10 weight portions, sodium thiosulfate 155 weight portions, ascorbic acid 10 weight portions, disodiumedetate 115 weight portions, ethylenediaminetetraacetic acid 20 weight portions, citric acid 400 weight portions and tartaric acid 10 weight portions one or more;

Described transdermal enhancer is for being selected from Borneolum Syntheticum 215 weight portions, Camphora 20 weight portions, Mentholum 115 weight portions, menthol 10 weight portions, methyl salicylate 220 weight portions, laurocapram 10 weight portions, Decylmethyl Sulphoxide 440 weight portions, plant Oleum sesami 10 weight portions;

Described wetting agent is to be selected from glycerol 50 weight portions, Polyethylene Glycol 490 weight portions, sorbitol 10 weight portions, propylene glycol 245 weight portions, paraffin oil 20 weight portions, mineral oil 60 weight portions, paraffin 10 weight portions, squalane 125 weight portions, silicone oil aliphatic alcohol 10 weight portions and wax fat 160 weight portions one or more;

Described surfactant is to be selected from Polysorbate 195 weight portions and sodium lauryl sulphate 20 weight portions one or both;

Described antibacterial is to be selected from parabens 5 weight portions, phenethanol 25 weight portions, sodium benzoate 1 weight portion and sorbic acid 25 weight portions one or more.

14. the preparation method of one of a claim 1-13 described gel may further comprise the steps:

4) gel skeleton material adding distil water is soaked, make its abundant swelling, add one or more adjuvants that are selected from pH regulator agent, stabilizing agent, transdermal enhancer, wetting agent, surfactant and the antibacterial selectively, stir, make gel-type vehicle; And

15. preparation method according to claim 14, wherein,

In step 2) in, adopt water boiling and extraction method, ethanol reflux extraction, ethanol percolate extraction method or alcohol dipping extraction method to extract.

16. according to claim 14 or 15 described preparation methoies, wherein, in step 2) in,

Take by weighing the 10%-100% of above-mentioned mixed material medicine gross weight, decocting with the 5-20 times of weight boils 1-4 time, decocts collection, merge extractive liquid, and volatile oil 0.5-3 hour at every turn, it is the concentrated solution of 1.0-1.5 that filtration and concentrated extracting solution become relative density, gets concentrated solution a; Perhaps

Take by weighing the 10%-100% of above-mentioned mixed material medicine gross weight, decocting with the 5-20 times of weight boils 1-4 time, the each decoction 0.5-3 hour, collection, merge extractive liquid, and volatile oil filter, and the adsorption column of macroporous adsorbent resin is housed filtrate adding, with 30-85% ethanol 10-50 times of weight eluting, collect eluent, be condensed into the concentrated solution that relative density is 1.0-1.5, and with volatile oil join in the concentrated solution concentrated solution a; Perhaps

Take by weighing the 10%-100% of above-mentioned mixed material medicine gross weight, the 30-90% alcohol reflux of usefulness 5-20 times of weight 1-4 time extracted 0.5-24 hour at every turn, collected extracting solution, filtered, and being condensed into relative density is the concentrated solution a of 1.0-1.5; Perhaps

Take by weighing the 10%-100% of above-mentioned mixed material medicine gross weight, the 30-90% ethanol that adds medicated powder weight 0.6-1.2 times of weight stirs and soaked into 0.5-2 hour, pack in the percolation jar, 30-90% ethanol percolation with the 5-20 times of weight, collect, merge percolate, filter, it is the concentrated solution a of 1.0-1.5 that concentrating under reduced pressure becomes relative density; Perhaps

Take by weighing the 10%-100% of above-mentioned mixed material medicine gross weight, extract 1-4 time with the 30-90% alcohol dipping of 5-20 times of weight, flooded 12-24 hour at every turn, collect, merge impregnation liquid, filter, it is the concentrated solution a of 1.0-1.5 that concentrating under reduced pressure becomes relative density.

17. preparation method according to claim 16, wherein, in step 2) in,

Take by weighing 10% of above-mentioned mixed material medicine gross weight, boil 3 times with 6 times of weight decoctings, decocted collection, merge extractive liquid, and volatile oil at every turn 1 hour, filter and concentrated extracting solution to become relative density be the concentrated solution of 1.25-1.35, and with volatile oil join in the concentrated solution concentrated solution a; Perhaps

Take by weighing 10% of above-mentioned mixed material medicine gross weight, decocting with 6 times of weight boils 3 times, the each decoction 1 hour, collection, merge extractive liquid, and volatile oil filter, and the adsorption column of macroporous adsorbent resin is housed filtrate adding, with 15 times of amounts of 35% ethanol eluting, collect eluent, be condensed into the concentrated solution that relative density is 1.25-1.35, and with volatile oil join in the concentrated solution concentrated solution a; Perhaps

Take by weighing 10% of above-mentioned mixed material medicine gross weight, with 40% alcohol reflux of 6 times of weight 3 times, extracted 1 hour at every turn, collect, merge extractive liquid,, filter, being condensed into relative density is the concentrated solution a of 1.25-1.35; Perhaps

Take by weighing 10% of above-mentioned mixed material medicine gross weight, 40% ethanol that adds medicated powder weight 0.8 times of weight stirs and soaked into 1 hour, in the percolation jar of packing into, ethanol percolation with 6 times of weight 40%, collect, merge percolate, filter, being evaporated to relative density is the concentrated solution a of 1.25-1.35; Perhaps

Take by weighing 10% of above-mentioned mixed material medicine gross weight, extract 3 times with 40% alcohol dipping of 6 times of weight, flooded 16 hours at every turn, collect, merge impregnation liquid, filtering and be evaporated to relative density is the concentrated solution a of 1.25-1.35; Perhaps

Take by weighing 20% of above-mentioned mixed material medicine gross weight, boil 2 times with 8 times of weight decoctings, decocted collection, merge extractive liquid, and volatile oil at every turn 2 hours, filter and concentrated extracting solution to become relative density be the concentrated solution of 1.35-1.45, and with volatile oil join in the concentrated solution concentrated solution a; Perhaps

Take by weighing 20% of above-mentioned mixed material medicine gross weight, decocting with 8 times of weight boils 2 times, the each decoction 2 hours, collection, merge extractive liquid, and volatile oil filter, and the adsorption column of macroporous adsorbent resin is housed filtrate adding, with 20 times of amounts of 45% ethanol eluting, collect eluent, be condensed into the concentrated solution that relative density is 1.35-1.45, and with volatile oil join in the concentrated solution concentrated solution a; Perhaps

Take by weighing 20% of above-mentioned mixed material medicine gross weight, with 50% alcohol reflux of 8 times of weight 2 times, extracted 2 hours at every turn, collect, merge extractive liquid,, filter, being condensed into relative density is the concentrated solution a of 1.35-1.45; Perhaps

Take by weighing 20% of above-mentioned mixed material medicine gross weight, 50% ethanol that adds medicated powder weight 0.9 times of weight stirs and soaked into 1.5 hours, in the percolation jar of packing into, ethanol percolation with 8 times of weight 50%, collect, merge percolate, filter, being evaporated to relative density is the concentrated solution a of 1.0-1.5; Perhaps

Take by weighing 20% of above-mentioned mixed material medicine gross weight, extract 2 times with 50% alcohol dipping of 8 times of weight, flooded 18 hours at every turn, collect, merge impregnation liquid, filtering and be evaporated to relative density is the concentrated solution a of 1.35-1.45; Perhaps

Take by weighing 50% of above-mentioned mixed material medicine gross weight, boil 2 times with 10 times of weight decoctings, decocted collection, merge extractive liquid, and volatile oil at every turn 1.5 hours, filter and concentrated extracting solution to become relative density be the concentrated solution of 1.15-1.25, and with volatile oil join in the concentrated solution concentrated solution a; Perhaps

Take by weighing 50% of above-mentioned mixed material medicine gross weight, decocting with 10 times of weight boils 2 times, the each decoction 1.5 hours, collection, merge extractive liquid, and volatile oil filter, and the adsorption column of macroporous adsorbent resin is housed filtrate adding, with 25 times of amounts of 50% ethanol eluting, collect eluent, be condensed into the concentrated solution that relative density is 1.15-1.25, and with volatile oil join in the concentrated solution concentrated solution a; Perhaps

Take by weighing 50% of above-mentioned mixed material medicine gross weight, with 60% alcohol reflux of 10 times of weight 2 times, extracted 1.5 hours at every turn, collect extracting solution, filter, being condensed into relative density is the concentrated solution a of 1.15-1.25; Perhaps

Take by weighing 50% of above-mentioned mixed material medicine gross weight, 60% ethanol that adds medicated powder weight 1.0 times of weight stirs and soaked into 0.5 hour, in the percolation jar of packing into, ethanol percolation with 10 times of weight 60%, collect, merge percolate, filter, being evaporated to relative density is the concentrated solution a of 1.15-1.25; Perhaps

Take by weighing 50% of above-mentioned mixed material medicine gross weight, extract 2 times with 60% alcohol dipping of 10 times of weight, flooded 20 hours at every turn, collect, merge impregnation liquid, filtering and be evaporated to relative density is the concentrated solution a of 1.15-1.25; Perhaps

Take by weighing 60% of above-mentioned mixed material medicine gross weight, boil 1 time with 12 times of weight decoctings, decocted collection, merge extractive liquid, and volatile oil 3 hours, filter and concentrated extracting solution to become relative density be the concentrated solution of 1.05-1.15, and with volatile oil join in the concentrated solution concentrated solution a; Perhaps

Take by weighing 60% of above-mentioned mixed material medicine gross weight, decocting with 12 times of weight boils 1 time, the each decoction 3 hours, collection, merge extractive liquid, and volatile oil filter, and the adsorption column of macroporous adsorbent resin is housed filtrate adding, with 30 times of amounts of 60% ethanol eluting, collect eluent, be condensed into the concentrated solution that relative density is 1.05-1.15, and with volatile oil join in the concentrated solution concentrated solution a; Perhaps

Take by weighing 60% of above-mentioned mixed material medicine gross weight, with 75% alcohol reflux of 12 times of weight 1 time, extracted 24 hours, collect, merge extractive liquid,, filter, being condensed into relative density is the concentrated solution a of 1.05-1.15; Perhaps

Take by weighing 60% of above-mentioned mixed material medicine gross weight, 75% ethanol that adds medicated powder weight 0.8 times of weight stirs and soaked into 1 hour, in the percolation jar of packing into, ethanol percolation with 12 times of weight 75%, collect, merge percolate, filter, being evaporated to relative density is the concentrated solution a of 1.05-1.15; Perhaps

Take by weighing 60% of above-mentioned mixed material medicine gross weight, extract 1 time with 75% alcohol dipping of 12 times of weight, flooded 24 hours, collect, merge impregnation liquid, filtering and be evaporated to relative density is the concentrated solution a of 1.05-1.15; Perhaps

Take by weighing 90% of above-mentioned mixed material medicine gross weight, boil 4 times with 15 times of weight decoctings, decocted collection, merge extractive liquid, and volatile oil at every turn 0.5 hour, filter and concentrated extracting solution to become relative density be the concentrated solution of 1.15-1.25, and with volatile oil join in the concentrated solution concentrated solution a; Perhaps

Take by weighing 90% of above-mentioned mixed material medicine gross weight, decocting with 15 times of weight boils 4 times, the each decoction 0.5 hour, collection, merge extractive liquid, and volatile oil filter, and the adsorption column of macroporous adsorbent resin is housed filtrate adding, with 35 times of amounts of 70% ethanol eluting, collect eluent, be condensed into the concentrated solution that relative density is 1.15-1.25, and with volatile oil join in the concentrated solution concentrated solution a; Perhaps

Take by weighing 90% of above-mentioned mixed material medicine gross weight, with 80% alcohol reflux of 15 times of weight 4 times, extracted 0.5 hour at every turn, collect extracting solution, filter, being condensed into relative density is the concentrated solution a of 1.15-1.25; Perhaps

Take by weighing 90% of above-mentioned mixed material medicine gross weight, 80% ethanol that adds medicated powder weight 0.6 times of weight stirs and soaked into 5 hours, in the percolation jar of packing into, 80% ethanol percolation with 15 times of weight, collect, merge percolate, filter, being evaporated to relative density is the concentrated solution a of 1.15-1.25; Perhaps

Take by weighing 90% of above-mentioned mixed material medicine gross weight, extract 4 times with 80% alcohol dipping of 15 times of weight, flooded 12 hours at every turn, collect, merge impregnation liquid, filtering and be evaporated to relative density is the concentrated solution a of 1.15-1.25; Perhaps

Take by weighing above-mentioned mixed material medicine, boil 3 times, decocted 2 hours at every turn with 10 times of weight decoctings, collection, merge extractive liquid, and volatile oil, filter and concentrated extracting solution to become relative density be the concentrated solution of 1.05-1.15, and with volatile oil join in the concentrated solution concentrated solution a; Perhaps

Take by weighing above-mentioned mixed material medicine, decocting with 10 times of weight boils 3 times, the each decoction 2 hours, collection, merge extractive liquid, and volatile oil filter, and the adsorption column of macroporous adsorbent resin is housed filtrate adding, with 40 times of amounts of 80% ethanol eluting, collect eluent, be condensed into the concentrated solution that relative density is 1.05-1.15, and with volatile oil join in the concentrated solution concentrated solution a; Perhaps

Take by weighing above-mentioned mixed material medicine, use 60% alcohol reflux 3 times of 10,8,6 times of medicine gross weights respectively, extracted respectively 90,60,30 minutes at every turn, collect, merge extractive liquid,, filter, being condensed into relative density is the concentrated solution a of 1.05-1.15; Perhaps

Take by weighing above-mentioned mixed material medicine, 60% ethanol that adds medicated powder weight 1.0 times of weight stirs and soaked into 1 hour, in the percolation jar of packing into, 60% ethanol percolation with 10 times of weight, collect, merge percolate, filter, being evaporated to relative density is the concentrated solution a of 1.05-1.15; Perhaps

Take by weighing above-mentioned mixed material medicine, extract 3 times with 60% alcohol dipping of 10 times of weight, flooded 18 hours at every turn, collect, merge impregnation liquid, filtering and be evaporated to relative density is the concentrated solution a of 1.05-1.15.

18. the preparation method of one of a claim 1-13 described gel may further comprise the steps:

3) gel skeleton material adding distil water is soaked, make its abundant swelling, add one or more adjuvants that are selected from pH regulator agent, stabilizing agent, transdermal enhancer, wetting agent, surfactant and the antibacterial selectively, stir, make gel-type vehicle; And

19. preparation method according to claim 18, wherein, in step 1),

Herba Oxytropis Kansuensis, sub-rhubarb extract, Radix aconiti szechenyiani, Fructus Chebulae (pitted), Fructus Terminaliae Billericae, Fructus Phyllanthi, Benzoinum, Herba Lycopodii are mixed, be ground into the medicated powder that particle diameter is 0.1-1 μ m; Perhaps

Herba Oxytropis Kansuensis, sub-rhubarb extract, Radix aconiti szechenyiani, Fructus Chebulae (pitted), Fructus Terminaliae Billericae, Fructus Phyllanthi, Benzoinum, Herba Lycopodii are mixed, be ground into the medicated powder that particle diameter is 1-10 μ m; Perhaps

Herba Oxytropis Kansuensis, sub-rhubarb extract, Radix aconiti szechenyiani, Fructus Chebulae (pitted), Fructus Terminaliae Billericae, Fructus Phyllanthi, Benzoinum, Herba Lycopodii are mixed, be ground into the medicated powder that particle diameter is 10-40 μ m; Perhaps

Herba Oxytropis Kansuensis, sub-rhubarb extract, Radix aconiti szechenyiani, Fructus Chebulae (pitted), Fructus Terminaliae Billericae, Fructus Phyllanthi, Benzoinum, Herba Lycopodii are mixed, be ground into the medicated powder that particle diameter is 40-60 μ m; Perhaps

Herba Oxytropis Kansuensis, sub-rhubarb extract, Radix aconiti szechenyiani, Fructus Chebulae (pitted), Fructus Terminaliae Billericae, Fructus Phyllanthi, Benzoinum, Herba Lycopodii are mixed, be ground into the medicated powder that particle diameter is 60-90 μ m; Perhaps

Herba Oxytropis Kansuensis, sub-rhubarb extract, Radix aconiti szechenyiani, Fructus Chebulae (pitted), Fructus Terminaliae Billericae, Fructus Phyllanthi, Benzoinum, Herba Lycopodii are mixed, be ground into the medicated powder that particle diameter is 90-150 μ m.