CN102978000B - Depickling method of antarctic krill oil - Google Patents
Depickling method of antarctic krill oil Download PDFInfo
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- CN102978000B CN102978000B CN201210457027.4A CN201210457027A CN102978000B CN 102978000 B CN102978000 B CN 102978000B CN 201210457027 A CN201210457027 A CN 201210457027A CN 102978000 B CN102978000 B CN 102978000B
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- 229940106134 krill oil Drugs 0.000 title claims abstract description 49
- 238000000034 method Methods 0.000 title claims abstract description 34
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims abstract description 108
- 239000002253 acid Substances 0.000 claims abstract description 23
- 239000011259 mixed solution Substances 0.000 claims abstract description 19
- 238000003756 stirring Methods 0.000 claims abstract description 18
- 238000001816 cooling Methods 0.000 claims abstract description 9
- 238000010438 heat treatment Methods 0.000 claims abstract description 5
- 238000002386 leaching Methods 0.000 claims description 16
- 238000013517 stratification Methods 0.000 claims description 16
- 238000012545 processing Methods 0.000 abstract description 4
- 239000006071 cream Substances 0.000 abstract 5
- 238000003811 acetone extraction Methods 0.000 abstract 1
- 239000000243 solution Substances 0.000 abstract 1
- 238000001291 vacuum drying Methods 0.000 abstract 1
- 241000239366 Euphausiacea Species 0.000 description 8
- 235000020777 polyunsaturated fatty acids Nutrition 0.000 description 6
- 238000009874 alkali refining Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 3
- 235000021323 fish oil Nutrition 0.000 description 3
- 150000003904 phospholipids Chemical class 0.000 description 3
- 238000007670 refining Methods 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 241000238557 Decapoda Species 0.000 description 2
- 230000004071 biological effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 235000019197 fats Nutrition 0.000 description 2
- 239000004519 grease Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 235000020660 omega-3 fatty acid Nutrition 0.000 description 2
- 239000010913 used oil Substances 0.000 description 2
- 239000003643 water by type Substances 0.000 description 2
- 241000239370 Euphausia superba Species 0.000 description 1
- 208000031226 Hyperlipidaemia Diseases 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000000747 cardiac effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000008157 edible vegetable oil Substances 0.000 description 1
- 210000003754 fetus Anatomy 0.000 description 1
- 235000021588 free fatty acids Nutrition 0.000 description 1
- 125000005908 glyceryl ester group Chemical group 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 238000004393 prognosis Methods 0.000 description 1
- 235000015067 sauces Nutrition 0.000 description 1
- 208000010110 spontaneous platelet aggregation Diseases 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- Coloring Foods And Improving Nutritive Qualities (AREA)
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Abstract
The invention discloses a depickling method of antarctic krill oil. The depickling method of the antarctic krill oil comprises the following steps of (1) adding acetone into the antarctic krill oil which is heated to the temperature in the range of 20 DEG C to 80 DEG C, wherein the temperature of the acetone is preheated to the same temperature of the antarctic krill oil and added volume of the acetone is 1to 10 times of that of the antarctic krill oil, stirring and extracting under the heated temperature for 10minutes to 300 minutes, cooling the mixed solution to 0 to 10 DEG C, standing and layering at the cooling temperature for 10 minutes to 600 minutes until the solution is layered, taking cream of the lower layer, (2) heating the cream to the temperature of 30 DEG C to 80 DEG C, adding the acetone with the same temperature of the cream, wherein the volume of the acetone is 1 to 10 times of that of the cream, stirring and extracting under the heated temperature for 10 minutes to 300 minutes, cooling the mixed solution to 0 DEG C to 20 DEG C, standing and layering under the cooling temperature for 5 minutes to 600 minutes until the mixed solution is layered, (3) taking the cream obtained by layering of the lower layer at the last of step 2, removing acetone through vacuum drying method, and obtaining low acid value antarctic krill oil. The depickling method of the antarctic krill oil builds up depickling process which is applicable to the antarctic krill oil for the first time, and acetone extraction method is used for carrying out depickling processing to the antarctic krill oil.
Description
Technical field
The present invention relates to the deep processing of antarctic krill oil, more particularly, relate to acid stripping method.
Background technology
Krill (Euphausia superba) is single one of Biological resources of planting maximum on the earth, the estimated value of its stock on hand is about 4～1,500,000,000 tons, ripe shrimp annual production is 3～500,000,000 tons, but a year quantity of the catch can reach 100,000,000 tons of left and right, forms huge potential fishery resources.In recent years, along with the exhaustion gradually of worldwide traditional fishery resource, and the proposition of 200-nautical-mile exclusive economic zone, make krill resource huge in antarctic waters receive the concern of some sea going fisheries developed countries.China also lists the krill resource in one of main exploitation kind of Development for Distant Water Fishery from now on.
Krill is lived in the waters, frigid zone, in shrimp sauce, is rich in to take the phosphatide type omega-3 polyunsaturated fatty acid that DHA and EPA be representative.Research shows, antarctic krill phospholipid type omega-3 polyunsaturated fatty acid has wholesome effect widely, comprises the prognosis that improves the cardiac, increases the speed of growth of fetus, the growth that suppresses tumour and transfer, anti-inflammatory, platelet aggregation-against, hypertension and hyperlipidemia and regulates body to the susceptibility of Regular Insulin etc.This shows, nutrition and the pharmaceutical use of antarctic krill oil are high, has fine and exploitation prospect.
Krill is rich in free fatty acids, causes the acid value of antarctic krill oil higher, higher than the acid value standard of the existing edible oil of country and fish oil.Develop the krill oil production with edible safety, the depickling of antarctic krill oil is imperative.Industrial normally used oil and fat refining process, first the come unstuck technique of rear depickling of general employing, first by the process of coming unstuck except the phosphatide in degrease, adopt again the depickling of alkali refining method, otherwise the phosphatide in grease is the meeting water-swelling when the depickling of alkali refining method, affects deacidification effect.Different from other greases, antarctic krill oil is rich in phosphatide, and antarctic krill phospholipid is rich in the phosphatide type polyunsaturated fatty acid.Research shows, with glyceryl ester type polyunsaturated fatty acid, compares, and the bioavailability of phosphatide type polyunsaturated fatty acid is higher, biological activity is better.The phosphatide type polyunsaturated fatty acid is the greatest feature that antarctic krill oil is different from traditional fish oil product.To sum up tell, due to the singularity of antarctic krill oil, industrial alkali refining method deacidifying process commonly used also is not suitable for the depickling of antarctic krill oil, is applicable to the acid stripping method of antarctic krill oil in the urgent need to foundation.
Summary of the invention
The present invention aims to provide a kind of method of depickling of applicable antarctic krill oil, has the advantages that deacidification efficiency is high.
In order to achieve the above object, the invention provides a kind of acid stripping method of antarctic krill oil, comprise the steps
Step1: to being heated in the antarctic krill oil of 20-80 ℃, add the acetone that is preheating to uniform temp, acetone add 1-10 that volume is antarctic krill oil doubly, and under Heating temperature stirring and leaching 10-300 minute, mixed solution is cooled to 0-10 ℃, and under cooling temperature stratification 10-600 minute, treat layering, take off a layer paste;
Step2: described paste is heated to 30-80 ℃, add the acetone that is preheating to uniform temp, acetone add 1-10 that volume is paste doubly, stirring and leaching 10-300 minute under Heating temperature, mixed solution is cooled to 0-20 ℃, and under cooling temperature stratification 5-600 minute; Treat layering;
Step3, get lower floor's paste of the last layering of S2, through the de-acetone treatment of boulton process, must hang down the acid value antarctic krill oil.
Under optimal way, in order to improve acid removal rate, to Step2 gained lower floor paste, then this step 1-2 time repeatedly, obtain lower floor's paste of last layering.
Industrial normally used oil and fat refining process, generally first remove the phosphatide in degrease by degumming technology, then adopt the depickling of alkali refining method at present, otherwise the phosphatide in grease is the meeting water-swelling when the depickling of alkali refining method, affects deacidification effect.Antarctic krill oil is rich in phosphatide, and antarctic krill oil phosphatide is rich in the phosphatide type polyunsaturated fatty acid.Research shows, the bioavailability of antarctic krill phospholipid type polyunsaturated fatty acid is higher, biological activity is better, is the greatest feature that antarctic krill oil is different from traditional fish oil product.Visible, traditional oil refining process also is not suitable for the depickling of antarctic krill oil.The present invention sets up the deacidifying process that is applicable to antarctic krill oil first, has overcome the phosphatide loss that traditional acid stripping method brings; Adopt the acetone extract method to carry out the depickling processing to antarctic krill oil, acid removal rate can reach more than 60%.
Embodiment
Embodiment 1:
Add to being heated in the antarctic krill oil of 50 ℃ the acetone that is preheating to uniform temp, 5 times (volume ratios) that the add-on of acetone is antarctic krill oil, 50 ℃ of lower stirring and leaching 60 minutes, mixed solution is cooled to 4 ℃, and at this temperature stratification 180 minutes, after layering, remove the upper strata acetone soln.
Lower floor's paste is heated to 50 ℃, adds the acetone that is preheating to uniform temp, 5 times (volume ratios) that the add-on of acetone is paste, 50 ℃ of lower stirring and leaching 60 minutes, be cooled to 4 ℃ by mixed solution, and at this temperature stratification 180 minutes.After layering, remove the upper strata acetone soln.
Lower floor's paste is heated to 50 ℃, adds the acetone that is preheating to uniform temp, 5 times (volume ratios) that the add-on of acetone is paste, 50 ℃ of lower stirring and leaching 60 minutes, be cooled to 4 ℃ by mixed solution, and at this temperature stratification 180 minutes.After layering, remove the upper strata acetone soln.
Lower floor's paste is heated to 50 ℃, adds the acetone that is preheating to uniform temp, 5 times (volume ratios) that the add-on of acetone is paste, 50 ℃ of lower stirring and leaching 60 minutes, be cooled to 4 ℃ by mixed solution, and at this temperature stratification 180 minutes.After layering, remove the upper strata acetone soln.Lower floor's paste obtains low acid value antarctic krill oil after the de-acetone treatment of boulton process, and acid removal rate is 93.71%.
Embodiment 2:
Add to being heated in the antarctic krill oil of 40 ℃ the acetone that is preheating to uniform temp, 4 times (volume ratios) that the add-on of acetone is antarctic krill oil, 40 ℃ of lower stirring and leaching 150 minutes, mixed solution is cooled to 5 ℃, and at this temperature stratification 200 minutes, after layering, remove the upper strata acetone soln.Lower floor's paste is heated to 40 ℃, adds the acetone that is preheating to uniform temp, 4 times (volume ratios) that the add-on of acetone is paste, 40 ℃ of lower stirring and leaching 150 minutes, be cooled to 5 ℃ by mixed solution, and at this temperature stratification 200 minutes.After layering, remove the upper strata acetone soln.Lower floor's paste obtains low acid value antarctic krill oil after the de-acetone treatment of boulton process, and acid removal rate is 60.64%.
Embodiment 3:
Add to being heated in the antarctic krill oil of 35 ℃ the acetone that is preheating to uniform temp, 2 times (volume ratios) that the add-on of acetone is antarctic krill oil, 35 ℃ of lower stirring and leaching 120 minutes, mixed solution is cooled to 8 ℃, and under cooling temperature stratification 180 minutes, after layering, remove the upper strata acetone soln.
Lower floor's paste is heated to 55 ℃, adds the acetone that is preheating to uniform temp, 2 times (volume ratios) that the add-on of acetone is paste, 55 ℃ of lower stirring and leaching 150 minutes, be cooled to 8 ℃ by mixed solution, and at this temperature stratification 180 minutes.After layering, remove the upper strata acetone soln.
Lower floor's paste is heated to 55 ℃, adds the acetone that is preheating to uniform temp, 2 times (volume ratios) that the add-on of acetone is paste, 55 ℃ of lower stirring and leaching 150 minutes, be cooled to 8 ℃ by mixed solution, and at this temperature stratification 180 minutes.After layering, remove the upper strata acetone soln.Lower floor's paste obtains low acid value antarctic krill oil after the de-acetone treatment of boulton process, and acid removal rate is 78.78%.
Embodiment 4:
Add to being preheating in the antarctic krill oil of 35 ℃ the acetone that is preheating to uniform temp, 4 times (volume ratios) that the add-on of acetone is antarctic krill oil, 35 ℃ of lower stirring and leaching 45 minutes, mixed solution is cooled to 4 ℃, and at this temperature stratification 360 minutes, after layering, remove the upper strata acetone soln.
Lower floor's paste is heated to 55 ℃, adds the acetone that is preheating to uniform temp, 4 times (volume ratios) that the add-on of acetone is paste, 55 ℃ of lower stirring and leaching 120 minutes, be cooled to 4 ℃ by mixed solution, and at this temperature stratification 180 minutes.After layering, remove the upper strata acetone soln.
Lower floor's paste is heated to 55 ℃, adds the acetone that is preheating to uniform temp, 4 times (volume ratios) that the add-on of acetone is paste, 55 ℃ of lower stirring and leaching 120 minutes, be cooled to 4 ℃ by mixed solution, and at this temperature stratification 180 minutes.After layering, remove the upper strata acetone soln.Lower floor's paste obtains low acid value antarctic krill oil after the de-acetone treatment of boulton process, and acid removal rate is 85.41%.
By the contrast of above-described embodiment, the processing parameter of embodiment 1 is preferred version of the present invention, and its acid removal rate significantly is better than other embodiment.
The above; it is only preferably embodiment of the present invention; but protection scope of the present invention is not limited to this; anyly be familiar with those skilled in the art in the technical scope that the present invention discloses; be equal to replacement or changed according to technical scheme of the present invention and inventive concept thereof, within all should being encompassed in protection scope of the present invention.
Claims (2)
1. the acid stripping method of an antarctic krill oil, is characterized in that, comprises the steps
S1: to being heated in the antarctic krill oil of 20-80 ℃, add the acetone that is preheating to uniform temp, acetone add 1-10 that volume is antarctic krill oil doubly, and under Heating temperature stirring and leaching 10-300 minute, mixed solution is cooled to 0-10 ℃, and under cooling temperature stratification 10-600 minute, treat layering, take off a layer paste;
S2: described paste is heated to 30-80 ℃, add the acetone that is preheating to uniform temp, acetone add 1-10 that volume is paste doubly, stirring and leaching 10-300 minute under Heating temperature, mixed solution is cooled to 0-20 ℃, and under cooling temperature stratification 5-600 minute; Treat layering, obtain lower floor's paste;
S3: get lower floor's paste of the last layering of S2, through the de-acetone treatment of boulton process, must hang down the acid value antarctic krill oil.
2. the acid stripping method of antarctic krill oil according to claim 1, is characterized in that, to S2 gained lower floor paste, then S2 step 1-2 time repeatedly, obtains lower floor's paste of last layering.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210457027.4A CN102978000B (en) | 2012-11-14 | 2012-11-14 | Depickling method of antarctic krill oil |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210457027.4A CN102978000B (en) | 2012-11-14 | 2012-11-14 | Depickling method of antarctic krill oil |
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| Publication Number | Publication Date |
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| CN102978000A CN102978000A (en) | 2013-03-20 |
| CN102978000B true CN102978000B (en) | 2014-01-08 |
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| CN111088113A (en) * | 2020-02-11 | 2020-05-01 | 福建师范大学 | Euphausia superba oil extraction and refining process |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101573040A (en) * | 2006-06-28 | 2009-11-04 | 丢米尔焦联合股份有限公司 | Deacidification method |
| CN102439125A (en) * | 2010-12-03 | 2012-05-02 | 上海赢贝生物技术有限公司 | Method for preparing high quality antarctic krill oil of phosphatidylserine containing unsaturated double bond in the fatty acyl |
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2012
- 2012-11-14 CN CN201210457027.4A patent/CN102978000B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101573040A (en) * | 2006-06-28 | 2009-11-04 | 丢米尔焦联合股份有限公司 | Deacidification method |
| CN102439125A (en) * | 2010-12-03 | 2012-05-02 | 上海赢贝生物技术有限公司 | Method for preparing high quality antarctic krill oil of phosphatidylserine containing unsaturated double bond in the fatty acyl |
Non-Patent Citations (4)
| Title |
|---|
| Acetone-Stable Nanofiltration Membranes in Deacidifying Vegetable Oil;H.J.ZWIJNENBERG ET AL.;《J.Am Oil Chem Soc》;19991231;第76卷(第1期);第83-87页 * |
| H.J.ZWIJNENBERG ET AL..Acetone-Stable Nanofiltration Membranes in Deacidifying Vegetable Oil.《J.Am Oil Chem Soc》.1999,第76卷(第1期),第83-87页. |
| 刘发义 等.超临界流体萃取技术在海洋生物活性物质提取中的应用.《海洋科学》.1996,(第4期),第41-44页. |
| 超临界流体萃取技术在海洋生物活性物质提取中的应用;刘发义 等;《海洋科学》;19961231(第4期);第41-44页 * |
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