CN102965957A - Wool product biological process mould-proof finishing technology based on cutinase pretreatment - Google Patents
Wool product biological process mould-proof finishing technology based on cutinase pretreatment Download PDFInfo
- Publication number
- CN102965957A CN102965957A CN2012104539337A CN201210453933A CN102965957A CN 102965957 A CN102965957 A CN 102965957A CN 2012104539337 A CN2012104539337 A CN 2012104539337A CN 201210453933 A CN201210453933 A CN 201210453933A CN 102965957 A CN102965957 A CN 102965957A
- Authority
- CN
- China
- Prior art keywords
- wool
- cutinase
- lysozyme
- certain
- mould
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention discloses a wool product biological process mould-proof finishing technology based on cutinase pretreatment, belonging to the technical field of wool fabric dyeing and finishing, which is aimed at realizing a mould-proof effect of a wool product by use of lysozyme. The technology disclosed by the invention can solve the problems of the traditional mould-proof method that a chemical mould inhibitor has toxicity and a colored mould inhibitor influences appearance and mould-proof effect of wool fabric and is not resistant to washing and the like. The technology comprises the following steps of: processing wool in a cutinase solution with certain concentration at certain temperature and pH value to remove a hydrophobic lipoid layer on the wool surface and improve the hydrophilicity of wool and expose a reactive group on the wool surface; impregnating the pretreated wool in a solution with certain pH value, and adsorbing lysozyme on wool fiber through electrostatic interaction; and processing the wool fiber with pre-adsorbed lysozyme in a glutaraldehyde solution with certain concentration at certain temperature for certain time, and fixing the lysozyme on the wool product through electrostatic adsorption and chemical crosslinking effect; and performing washing and low-temperature drying.
Description
Technical field
A kind of based on the pretreated wool by bio method of cutinase mildew resistant finish technique, belong to the applied technical field that woolen dyeing and finishing is processed in the wool textile industry.
Background technology
Mould is referred to as again filamentous fungi, have distribute wide, breeding is fast, strong adaptability and easy characteristics such as variation, is one of main biological factor that endangers wool textile.Mould is extensive in distributed in nature, the trace that it is arranged in soil, water, the air, in the south China area, because humiture is higher, the mildew phenomena of wool textile happens occasionally, and causes fabric impaired, after particularly seriously making moist, such as untimely processing mildew phenomena occuring very easily, causes serious loss.Wool textile surface mildew is a natural phenomena, and enzyme or other materials of mould secretion can damage wool textile, changes its physicochemical property and reduces service life.The textiles color and luster that goes mouldy changes, and can't restore, and can only abandon it, causes very large loss.And the musty that gives out in growth course of mould and the toxin of secretion also have harmful effect to health.Suck mycotic spore and can cause the mycotic pneumonia, especially the infant endangers especially severe.Therefore, production and application mildew-resistant wool textile has positive effect.
In recent years, the antimildew function of wool textile more and more causes people's attention.In order to reduce and eliminate the harm of mould, effective method is with various mould inhibitors wool textile to be processed, and can reach like this purpose of mildew-resistant.
The mould inhibitor that is used at present mildew resistant finish mainly contains inorganic metal compound such as Inorganic Copper compound (copper carbonate, cupric phosphate, copper fluoride, cupric oxide or cuprous oxide, copper formate etc.); Metal-organic complex such as oxine; (Ali's jar (unit of capacitance) C itself is not bactericide for organic compound such as salicylaniline, ethyl naphthol, dichlorophene, Ali's jar (unit of capacitance) C, but the preshrunk body of water miscible melamine resin, transfer molten with front not to be higher than 30 ℃ of warm water, and adding hydrogen peroxide, the solution Free-formaldehyde is oxidized to formic acid as catalyst, make simultaneously again it become acidic colloidal, its mildew-resistant mechanism is its low pH value, and mould fungus inhibition growth also makes fabric set up the protection isolation barrier), genuine 226 (ammonium chloride that contains the alkane 3,5-dimethylphenyl).But these mildew-proofing finishing agents, it is poisonous having many, all toxic such as Inorganic Copper compound, organic compound such as salicylaniline, ethyl naphthol, dichlorophene etc., Ali's jar (unit of capacitance) C contains a large amount of formaldehyde, the people is known from experience produce serious harmful effect; Many colors that also have are arranged, such as the Inorganic Copper compound, only can should not be used for interior dress material and light fabric for outdoor fluffy account series products.And these finishing agents can not react with wool fibre, can only be adsorbed on fiber surface by non-covalent bonding force, and its finishing effect is not durable, and once washing, its mildew resistant finish effect just exhausts.So these mildew-proofing finishing agents all can not be used for the mildew resistant finish of wool.
Lysozyme claims again cell membrane matter enzyme or N-acetylmuramide lycanohydrlase, has the enzyme of dissolution of bacteria cell membrane in nineteen twenty-two Britain bacteriologist A.Fleming finder's saliva, the tears, because it has bacteriolysis, so the called after lysozyme.After this in Various Tissues, juice and the certain plants of humans and animals, microorganism, also found the existence of lysozyme.Along with deepening continuously of research, find not only to have the lysozyme of dissolution of bacteria cell membrane, act in addition the lysozyme kind of fungal cell wall.
Lysozyme is divided into two large class, i.e. bacteria cell wall lysozyme and fungal cell wall lysozymes by its microbe species difference that acts on.The bacteria cell wall lysozyme has two kinds, and a kind of is the Lysozyme that acts on β-Isosorbide-5-Nitrae glycosidic bond, and another kind is the Lysozyme that acts on peptide " tail " and amide moieties.The fungal cell wall lysozyme comprises yeast cell wall lyase and fungal cell wall lyase.Lysozyme is distributed in occurring in nature widely, can find in people's tissue and secretion, also has in the animal tissue, and is maximum with content in the egg, also exists in other plant tissue and the microbial cell.Different according to the source, its character and mechanism of action are slightly variant.
Wool surface contains scale layer, and scale layer has complicated structure, can be divided into epicuticula, scale skin and scale internal layer.
Epicuticula claims again the epidermal cell thin layer, and thickness is about 0.9～2nm, and it is about 0.1% to account for the fiber total amount, has good chemical inertness, can be alkaline-resisting, the effect of oxidant, reductant and biology enzyme.The chemical stability of epicuticula is relevant with its unique chemical constitution.Be arranged with polarity and nonpolar lipides on the wool scale top layer, main 20 acid of 18-methyl and the LCFAs such as part C16, C18 by 70% consist of.Different aliphatic acid is by ester bond and serine, threonine, or links to each other with cystine residue by means of thioester bond, forms wool fibre surface water-repellent layer, makes wool have stronger hydrophobicity and stability.
This method is described a kind of based on the pretreated wool product bioanalysis of cutinase mildew resistant finish technique, at first be used to come from fungi or bacterium, can destroy the ester bond in the cutin polymer molecule and make its cutinase that is hydrolyzed to monomer and little molecule oligomer carry out preliminary treatment to wool, destroy the ester bond in the hydrophobicity lipoid structure of wool scale top layer, improve the hydrophily of wool surfaces, and the group that wool surfaces can be reacted exposes.The recycling electrostatic interaction is adsorbed onto lysozyme electronegative under the certain condition on the wool fibre of surface band positive charge; Then crosslinked being fixed on the wool fabric of fungal cell wall lysozyme of utilizing chemical method to have to dissolve the fungal cell wall effect stops growth, the breeding of mould on wool fabric, thereby reaches the purpose of mildew-resistant.
Lysozyme is a kind of natural preservative agent, has the characteristics such as safety non-toxic, Heat stability is good, thus the mildew-resistant woolen that the present invention produces to human body without any harmful effect, be a kind of safe, ecological function textile.And since adopt static adsorb in advance-the chemical crosslinking double action is fixed on lysozyme on the wool product, so finishing effect has durability.
Summary of the invention
Purpose of the present invention is intended to utilize biological mould inhibitor lysozyme to give the wool product anti-mold effect.It is toxic to use the present invention can solve the chemical anti mildew agent that traditional mold-proof method adopts, can produce serious harm to consumer's health, many mould inhibitors have color, can have a strong impact on the outward appearance of wool fabric, particularly present mould inhibitor all is non-response type, can only rely on non-covalent bonding force to be adsorbed on fiber surface and make not durable, the problem such as wash resistant not of anti-mold effect.Simultaneously, because the present invention has adopted cutinase preliminary treatment and the pre-adsorption treatment of static, can significantly improve the efficient of chemical cross-linking agent immobilized lysozyme, improve the anti-mold effect of lysozyme.
Technical scheme of the present invention: a kind of based on the pretreated wool product bioanalysis of cutinase mildew resistant finish technique, it is characterized in that wool fibre or wool top or woolen in containing the solution of certain density cutinase, under uniform temperature and certain pH value, process certain hour, remove the hydrophobicity lipoid layer of wool surfaces, improve the hydrophily of wool and the reactable group of wool surfaces is exposed; Then will be immersed in the certain pH value solution through pretreated wool fibre or wool top or wool fabric, since this pH value scope be in the wool isoelectric point above, below the lysozyme isoelectric point, so that lysozyme is positively charged and be adsorbed onto on the electronegative wool fibre by electrostatic interaction; And then will adsorb in advance the wool fibre that lysozyme is arranged and in certain density glutaraldehyde solution, process at a certain temperature certain hour, lysozyme is fixed on the wool product by Electrostatic Absorption and chemical crosslinking effect, wash at last, low temperature drying.
(1) cutinase preliminary treatment: wool fibre or wool top or wool fabric be impregnation process in containing the solution of wetting and penetrating agent that mass concentration is 0.5%～5.0% cutinase and 0～20g/L, 30～60 ℃ of temperature, pH 7.0～9.0, and 0.5～8 hour time, fabric is fully clean after processing; In cutinase solution during preliminary treatment, Determination of Polyoxyethylene Non-ionic Surfactants can be added or anion surfactant is made wetting and penetrating agent, to improve treatment fluid to the wettability of fiber; Also can not add any wetting and penetrating agent;
(2) Electrostatic Absorption: will be immersed in pH7.0～10.0 through the pretreated wool fibre of cutinase or wool top or wool fabric, concentration is in the lysozyme soln of 2-30g/L, and process 2-16h in 0-10 ℃, and take out, cold water fully washs 3-4 time;
(3) glutaraldehyde cross-linking is processed: will be through the cutinase preliminary treatment, adsorb wool fibre or wool top or woolen being immersed in the glutaraldehyde solution of 0.1%-1.0% of lysozyme, process 0.5-10h in 0-30 ℃, take out, cold water fully washs 3-4 time, 20-30 ℃ of oven dry;
Described a kind of based on the pretreated wool product bioanalysis of cutinase mildew resistant finish technique, the wool that is suitable for processing comprises wool fibre, wool tops and wool fabric.Wool fabric comprises worsted fabric and the various drap-de-berry fabrics such as all-wool gabardine, serge, valetin, women clothing.
The lysozyme of using is the fungal cell wall lysozyme.
Beneficial effect of the present invention: of the present invention a kind of based on the pretreated wool product bioanalysis of cutinase mildew resistant finish technique, it is toxic to use the present invention can solve the chemical anti mildew agent that traditional mold-proof method adopts, and can produce serious harm to consumer's health; Many mould inhibitors have color, can have a strong impact on the outward appearance of woolen; Particularly present mould inhibitor all is non-response type, can only rely on non-covalent bonding force to be adsorbed on wool surface and make not durable, the problem such as wash resistant not of anti-mold effect.Simultaneously, because the present invention has adopted the cutinase preliminary treatment, remove the lipoid layer of wool surface, improved hydrophily and the reactable of wool fibre; On this basis, in conjunction with the pre-adsorption treatment of static, the binding capacity of very high lysozyme on fiber, and then significantly improve the efficient of chemical cross-linking agent immobilized lysozyme, improve the anti-mold effect of lysozyme.
The specific embodiment
Embodiment 1
Sample kind: all-wool gabardine.
With all-wool gabardine be immersed in pH7.0, concentration is in the lysozyme soln of 5g/L,, takes out abundant cold water washing, 20 ℃ of oven dry in 4 ℃ of reaction 12h.
Wool fabric through above-mentioned processing, after testing, its mildew-resistant grade is 3 grades, again after 2 washings, its mildew-resistant grade is 4 grades, illustrate that the lysozyme that adsorbs on the wool fabric all washes off, (anti-mold effect divides totally 5 grades of 0-4 levels, 0 grade: amplify Microscopic observations and have no mildew amplifying about 50 times to have no longer included anti-mold effect; 1 grade: be invisible to the naked eye or be difficult to see mildew, the fungus growth area is less than 10%; 2 grades: naked eyes are obviously seen mildew, and mould is 10%-30% in the area coverage of sample surfaces; 3 grades: naked eyes are obviously seen mildew, and mould is 30%-60% in the area coverage of sample surfaces; 4 grades: naked eyes are obviously seen mildew, and mould is at the area coverage of sample surfaces＞60%).
Embodiment 2
Sample kind: all-wool gabardine.
With all-wool gabardine impregnation process in containing the solution of wetting and penetrating agent that mass concentration is 1.5% cutinase and 2g/L, 50 ℃ for the treatment of temperatures, pH 7.0, and 1.5 hours time, fabric is fully clean after processing.
To impregnated in pH7.0 through the wool fabric that cutinase is processed, concentration is in the lysozyme soln of 5g/L, in 4 ℃ of reaction 12h, take out, fully cold water washing.
The all-wool gabardine of again will be through the cutinase preliminary treatment, having adsorbed lysozyme is immersed in 0.2% the glutaraldehyde solution, processes 2h in 10 ℃, takes out, and cold water fully washs 3-4 time, 20 ℃ of oven dry.
Through the wool fabric of above-mentioned processing, after testing, its mildew-resistant grade is 1 grade, and after 2 washings, its mildew-resistant grade is 1 grade.Again after 10 washings, its mildew-resistant grade is 2 grades, after the pre-adsorption treatment of static through cutinase preliminary treatment and lysozyme is described, can make more lysozyme by glutaraldehyde and the crosslinked combination of wool fibre, and the lysozyme on the wool fabric mainly is to be combined with wool fibre with the form of covalent bond and ionic bond, although through fully washing, still remain with more lysozyme, good anti-mold effect arranged.
Claims (3)
1. one kind based on the pretreated wool product bioanalysis of cutinase mildew resistant finish technique, it is characterized in that wool fibre or wool top or woolen in containing certain density cutinase solution, under uniform temperature and certain pH value, process certain hour, remove the hydrophobicity lipoid layer of wool surface, improve the hydrophily of wool and the reactable group of wool surface is exposed; Then will be immersed in the certain pH value solution through pretreated wool, so that lysozyme is positively charged and be adsorbed onto on the electronegative wool fibre by electrostatic interaction; And then will adsorb in advance the wool fibre that lysozyme is arranged and in certain density glutaraldehyde solution, process at a certain temperature certain hour, lysozyme is fixed on the wool product by Electrostatic Absorption and chemical crosslinking effect, wash at last, low temperature drying;
(1) cutinase preliminary treatment: wool fibre or wool top or wool fabric be impregnation process in containing the solution of wetting and penetrating agent that mass concentration is 0.5%～5.0% cutinase and 0～20g/L, 30～60 ℃ for the treatment of temperatures, pH7.0～9.0,0.5～8 hour time, fiber or wool top or fabric are fully clean after processing; In cutinase solution during preliminary treatment, Determination of Polyoxyethylene Non-ionic Surfactants can be added or anion surfactant is made wetting and penetrating agent, to improve treatment fluid to the wettability of wool product; Also can not add any wetting and penetrating agent;
(2) Electrostatic Absorption: will be immersed in pH7.0～10.0 through the pretreated wool fibre of cutinase or wool top or wool fabric, concentration is in the lysozyme soln of 2-30g/L, and process 2-16h in 0-10 ℃, and take out, cold water fully washs 3-4 time;
(3) glutaraldehyde cross-linking is processed: will be through the cutinase preliminary treatment, adsorb wool fibre or wool top or woolen being immersed in the glutaraldehyde solution of 0.1%-1.0% of lysozyme, process 0.5-10h in 0-30 ℃, take out, cold water fully washs 3-4 time, 20-30 ℃ of oven dry.
2. according to claim 1 a kind of based on the pretreated wool product bioanalysis of cutinase mildew resistant finish technique, it is characterized in that the wool product that is suitable for processing comprises wool fibre, wool tops and wool fabric; Wool fabric comprises worsted fabric and the various drap-de-berry fabrics such as all-wool gabardine, serge, valetin, women clothing.
3. according to claim 1 a kind of based on the pretreated wool product bioanalysis of cutinase mildew resistant finish technique, it is characterized in that the cutinase of using can select the cutinase of separate sources, derive from the cutinase of fungi, bacterium or mould such as optional usefulness; The lysozyme of using should be the fungal cell wall lysozyme.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012104539337A CN102965957A (en) | 2012-10-31 | 2012-10-31 | Wool product biological process mould-proof finishing technology based on cutinase pretreatment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012104539337A CN102965957A (en) | 2012-10-31 | 2012-10-31 | Wool product biological process mould-proof finishing technology based on cutinase pretreatment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102965957A true CN102965957A (en) | 2013-03-13 |
Family
ID=47796373
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012104539337A Pending CN102965957A (en) | 2012-10-31 | 2012-10-31 | Wool product biological process mould-proof finishing technology based on cutinase pretreatment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102965957A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105040454A (en) * | 2015-07-23 | 2015-11-11 | 东华大学 | Biological treatment method for wool blend fabric |
| CN110016801A (en) * | 2019-04-18 | 2019-07-16 | 太和县欧得利毛发制品有限公司 | A kind of processing method before hair production |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006002034A1 (en) * | 2004-06-15 | 2006-01-05 | Novozymes North America, Inc | Simultaneous desizing and scouring process |
| US20060042020A1 (en) * | 2002-12-20 | 2006-03-02 | Novozymes North America, Inc. | Treatment of fabrics, fibers, or yarns |
| CN101424048A (en) * | 2008-11-11 | 2009-05-06 | 江南大学 | Technique for preventing wool loomage from felting by two-bath process using cutinase/protease |
| CN101424047A (en) * | 2008-11-11 | 2009-05-06 | 江南大学 | Method for antibacterial finishing loomage by immobilized lysozyme |
| CN101565899A (en) * | 2009-04-22 | 2009-10-28 | 江南大学 | Method for performing antibacterial finishing on wool fabric by utilizing transglutaminase to catalyze lysozyme immobilization |
-
2012
- 2012-10-31 CN CN2012104539337A patent/CN102965957A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060042020A1 (en) * | 2002-12-20 | 2006-03-02 | Novozymes North America, Inc. | Treatment of fabrics, fibers, or yarns |
| WO2006002034A1 (en) * | 2004-06-15 | 2006-01-05 | Novozymes North America, Inc | Simultaneous desizing and scouring process |
| CN101424048A (en) * | 2008-11-11 | 2009-05-06 | 江南大学 | Technique for preventing wool loomage from felting by two-bath process using cutinase/protease |
| CN101424047A (en) * | 2008-11-11 | 2009-05-06 | 江南大学 | Method for antibacterial finishing loomage by immobilized lysozyme |
| CN101565899A (en) * | 2009-04-22 | 2009-10-28 | 江南大学 | Method for performing antibacterial finishing on wool fabric by utilizing transglutaminase to catalyze lysozyme immobilization |
Non-Patent Citations (1)
| Title |
|---|
| 王平等: "Savinase和木瓜蛋白酶处理对羊毛性能的影响", 《纺织学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105040454A (en) * | 2015-07-23 | 2015-11-11 | 东华大学 | Biological treatment method for wool blend fabric |
| CN110016801A (en) * | 2019-04-18 | 2019-07-16 | 太和县欧得利毛发制品有限公司 | A kind of processing method before hair production |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Wang et al. | Antibacterial functionalization of wool fabric via immobilizing lysozymes | |
| CN105734981B (en) | A kind of method that laccase TEMPO methods carry out the wrinkle resistant antibiotic finish of real silk fabric | |
| CN107059413A (en) | Cutinase and laccase Combined Treatment carry out the function modified method of terylene | |
| CN105544198A (en) | Antibacterial and anti-felting wool fabric finishing method | |
| JP2001502014A (en) | Enzyme treatment to increase wettability and absorbency of fabric | |
| Sudan et al. | Production and biochemical characterization of xylanase from an alkalitolerant novel species Aspergillus niveus RS2 | |
| Dhiman et al. | Pretreatment processing of fabrics by alkalothermophilic xylanase from Bacillus stearothermophilus SDX | |
| Mostafa et al. | Enzymatic, kinetic and anti-microbial studies on Aspergillus terreus culture filtrate and Allium cepa seeds extract and their potent applications | |
| CN103031733A (en) | Preparation method of anti-crease antimicrobial complex-function true silk | |
| CN101424047A (en) | Method for antibacterial finishing loomage by immobilized lysozyme | |
| US20220002938A1 (en) | Method for anti-felting finishing of wool fabric with protease K | |
| CN102691214A (en) | Antibacterial finishing method for grafting lysozyme to laccase-catalyzed hemp fibers (fabrics) | |
| CN103556476B (en) | Multifunctional real silk and preparation method thereof | |
| CN103526554B (en) | A kind of enzymatic grafting lysozyme carries out the method for real silk antibiotic finish | |
| CN102965957A (en) | Wool product biological process mould-proof finishing technology based on cutinase pretreatment | |
| CN104745567A (en) | Immobilized spore laccase as well as preparation method and application thereof | |
| Khare et al. | The role of microbial enzyme systems in plant growth promotion | |
| CN104227811A (en) | Environment-friendly anti-mildew and antiseptic treatment method for bamboo board | |
| CN102644203B (en) | Method for improving antibacterial durability of ramie by laccase/phenols processing mode | |
| Jajpura | Enzyme: a bio catalyst for cleaning up textile and apparel sector | |
| CN102965956A (en) | Wool worsted fabric compound enzyme padding-steaming stacking anti-felting finishing technology | |
| Schirp et al. | Advances in understanding the ability of sapstaining fungi to produce cell wall-degrading enzymes | |
| CN104153200A (en) | Anti-felting method of cashmere fiber through enzymatic method | |
| CN101565899A (en) | Method for performing antibacterial finishing on wool fabric by utilizing transglutaminase to catalyze lysozyme immobilization | |
| CN102965955A (en) | Cutinase, keratinase and protease one-bath process anti-felting technology |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130313 |