CN102949437A - Traditional Chinese medicine for treating diabetic retinopathy and preparation method thereof - Google Patents
Traditional Chinese medicine for treating diabetic retinopathy and preparation method thereof Download PDFInfo
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- CN102949437A CN102949437A CN201210497399XA CN201210497399A CN102949437A CN 102949437 A CN102949437 A CN 102949437A CN 201210497399X A CN201210497399X A CN 201210497399XA CN 201210497399 A CN201210497399 A CN 201210497399A CN 102949437 A CN102949437 A CN 102949437A
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Abstract
The invention relates to a traditional Chinese medicine for treating diabetic retinopathy and a preparation method of the traditional Chinese medicinethereof, which relates to a traditional Chinese medicine preparation. The traditional Chinese medicine comprises the following raw materials: 5 to 15g of radix astragali, 2 to 8g of radix notoginseng, 3 to 7g of radix angelicae sinensis and 60 to 100ml of water; the traditional Chinese medicine for treating diabetic retinopathy is prepared by adding water into radix astragali, radix notoginseng and radix angelicae sinensis, boiling the mixture to obtain extracting solution after the soaking; placing the extracting solution into a traditional Chinese medicine storage tank, after the dregs are drained, concentrating the liquid medicine till each milliliter of the liquid medicine is equivalent to 1.1g of dried medicinal herbs, and thereby obtaining the traditional Chinese medicine for treating diabetic retinopathy. Based on the therapeutic principles of promoting circulation and removing stasis and warming channels and promoting coronary circulation, the traditional Chinese medicine for treating diabetic retinopathy is provided. The animal experiment research proves that the traditional Chinese medicine is capable of effectively inhibiting the inflammatory reaction inside rat retina suffering from type 2 diabetes mellitus, reducing the leukocyte adhesion, vascular permeability and the formation of the acellular blood vessels in the retinas caused by diabetes mellitus, thereby preventing the generation and/or the progress and exacerbation of the diabetic retinopathy.
Description
Technical field
The present invention relates to a kind of Chinese medicine preparation, especially relate to Chinese medicine of a kind for the treatment of of diabetic retinopathy change and its preparation method and application.
Background technology
Diabetic renal papillary necrosis (diabetic retinopathy, DR) is one of modal complication of diabetes, also is the most important reason of adult's blinding.Sickness rate prolongs with the increase of age and the course of disease, and the sickness rate of 3 years, 5 years, 10 years, the 15 years courses of disease of diabetes is respectively 8%, 25%, 60%, 80%, and blinding danger is higher 25 times than the ND.Its high incidence, high blind rate, patient's life quality in serious threat, is the key subjects that current medical domain is badly in need of solution.
Diabetic renal papillary necrosis is divided into non-proliferation period (simple type) and proliferation period (proliferous type).DR belongs to microangiopathies, and blood capillary refers to tiny blood vessels and the capillary network between small tremulous pulse and vein, is the place that tissue and blood carry out mass exchange.Retinal capillary is comprised of endotheliocyte, basement membrane, pericyte's three parts.Basic pathological changes comprises: 1. pericyte optionally loses; 2. basement membrane thickened; 3. the formation of microangioma; 4. endotheli ocytosis; 5. new vessels forms.Wherein, it is the earliest pathological change that retinal capillary pericytes is optionally lost, endotheliocyte basement membrane thickened, blood capillary automatic regulation function lose compensatory, the barrier function of endothelial cells infringement can appear thereupon, capillary permeability strengthens, thereby the generation blood constituent oozes out and blood capillary is inaccessible, causes retinal ischemia.And extensive retinal ischemia can further cause the formation of retinal edema and new vessels.In case new vessels occurs then indicate entering proliferation period.Proliferation period is to turn to principal character with retinal neovascularization formation and fiber.Because pathogenesis is not yet fully clear and definite, there is no at present for pathogenetic treatment means or the Drug therapy of determined curative effect.Non-proliferation period mainly takes to control blood glucose, blood pressure, blood fat, and oral drug therapy is even but glycemic control well can not stop generation and the development of DR fully.Proliferation period takes mainly that light is solidifying, cryocautery, and severe patient gives the oozy glass Corpectomy, but has complication and sequela, can cause fibrous tissue propagation, choroidal neovascularization, retinal hemorrhage and defect of visual field etc. under the retina such as laser therapy; Vitrectomy can cause recurrent vitreous hemorrhage, complicated cataract, iris neovascularization and neovascular glaucoma, tractional detachment of retina etc.Therefore be badly in need of at present strategy and the means that effectively prevention and treatment of diabetic retinopathy become, and particularly important to DR prevention and treatment at the onset diabetes initial stage.
Summary of the invention
The object of the present invention is to provide Chinese medicine of a kind for the treatment of of diabetic retinopathy change and preparation method thereof.
The raw material of the Chinese medicine that described a kind for the treatment of of diabetic retinopathy becomes consists of:
Wherein, the Radix Astragali, Radix Notoginseng and Radix Angelicae Sinensis are calculated in mass, and water is calculated by volume.
The raw material of the Chinese medicine that described a kind for the treatment of of diabetic retinopathy becomes forms can be preferably Radix Astragali 2g, Radix Notoginseng 1g, Radix Angelicae Sinensis 1g, water 25 ~ 50ml; Wherein, the Radix Astragali, Radix Notoginseng and Radix Angelicae Sinensis are calculated in mass, and water is calculated by volume.
The preparation method of the Chinese medicine that described a kind for the treatment of of diabetic retinopathy becomes may further comprise the steps:
1) Radix Astragali, Radix Notoginseng and Radix Angelicae Sinensis are added water, boil after the immersion, get extracting solution;
2) extracting solution is put into medicine storing pot, behind the diarrhea medicinal residues, be concentrated into every milliliter of medicinal liquid and be equivalent to the 1.1g crude drug, get the Chinese medicine that treatment of diabetic retinopathy becomes.
In step 1), the time of described immersion can be 20～40min, and described method of boiling can adopt microwave to boil the little 20～40min that boils of rear maintenance.
In step 2) in, described concentrated method can adopt opens vacuum, condensed water and microwave and concentrates in extraction pot, but the Chinese medicine packing 10ml/ bottle that the gained treatment of diabetic retinopathy becomes.
Through tested studies show that of the attached First Hospital diabetes study of Xiamen University, spontaneous type 2 diabetes mellitus model Goto-Kakizaki(GK) the blood glucose compared with normal Wistar rat of rat significantly increases, when 10 monthly ages, obvious blood capillary seepage occurring increases, leukocyte adhesion increases in the number of blood vessel wall, and a large amount of acellular inaccessible blood capillaries occur.The Chinese medicine that treatment of diabetic retinopathy of the present invention becomes (is called for short: Chinese angelica blood supplementing Tonga flavor side, code name: DG) formed by three flavor folk prescriptions (Radix Astragali, Radix Notoginseng, Radix Angelicae Sinensis), Main Function be invigorate blood circulation, blood stasis dispelling and collateral dredging, can effectively improve the above-mentioned pathological change of GK diabetic retinal tissue in rat.Trypan Blue shows, the retina seepage showed increased of GK rat, and DG can correct this phenomenon effectively.Long-term hyperglycemia causes the leukocyte adhesion rate to increase, leukocyte adhesion is piled up in the blood capillary, form little embolus, block blood capillary and discharge the various active material, increase the weight of microcirculation disturbance, cause that retinal capillary basement membrane thickened, blood-retina barrier are broken, blood capillary is inaccessible, DG can reduce the adhesion of leukocyte blood vessel wall in retina effectively, thereby reduces local leukocyte recruitment to blood vessel wall and damage blood-retina barrier.The formation of pericyte's apoptosis, forfeiture and acellular blood vessel has damaged the integrity of blood-retina barrier, also further increases the weight of amphiblestroid ischemia, anoxia, advances the process of diabetic renal papillary necrosis.Thereby the formation of acellular blood vessel stoped generation and/or the progress of DR when DG can reduce DR effectively.The improvement that DG changes retina pathology may be that the expression of intraretinal inflammatory factor realizes when suppressing diabetes.Above experimental result shows, amphiblestroid inflammatory reaction when this Chinese medicine preparation can suppress diabetes effectively reduces the formation of leukocyte adhesion, blood capillary seepage and acellular blood vessel, thereby prevents the generation of diabetic renal papillary necrosis or slow down its progress.
The present invention is take Traditional Chinese medicine blood circulation promoting blood stasis dispelling, warming the meridian and promoting blood circulation as the Therapeutic Principle, a kind of Chinese medicine that is used for the treatment of diabetic renal papillary necrosis is provided, animal experiment study confirms, inflammatory reaction in the Chinese medicine energy establishment type 2 diabetes mellitus rat retina of the present invention, the formation of intraretinal leukocyte adhesion, vascular leakage and acellular blood vessel due to the minimizing diabetes, thus stop the generation of diabetic renal papillary necrosis and/or progress to worsen.
Description of drawings
Fig. 1 is that DG is to the improvement of diabetic retina blood capillary seepage.In Fig. 1, CD: Doxium, DG: Chinese angelica blood supplementing Tonga flavor side,
*P＜0.05 vs.Control, #P＜0.05 vs.Diabetic, ##P＜0.01 v.Diabetic; Abscissa: grouping, vertical coordinate: the blue concentration (ng/mg) of Ivan scholar.
Fig. 2 is that Chinese angelica blood supplementing Tonga flavor side reduces leukocytic adhesion in the diabetic retina.In Fig. 2, A: Normal group; B: diabetic groups; C: the diabetes Chinese angelica blood supplementing Tonga side's of flavor treatment group; DG: Chinese angelica blood supplementing Tonga flavor side,
* *P＜0.001vs.Control, ###P＜0.001 vs.Diabetic vs.Diabetic; Abscissa: grouping, vertical coordinate: the leukocyte count Leukocytes/mm in the unit are
2
Fig. 3 is the analysis of accounts of leukocyte adhesion in the retina.In Fig. 3, DG: Chinese angelica blood supplementing Tonga flavor side,
* *P＜0.001vs.Control, ###P＜0.001 vs.Diabetic vs.Diabetic; Abscissa: grouping, vertical coordinate: the leukocyte count Leukocytes/mm in the unit are
2
Fig. 4 is the formation of the acellular blood vessel of retina when blood blood-enriching soup plus-minus reduces diabetes.In Fig. 4, A: Normal group; B: diabetic groups; C: the diabetes Chinese angelica blood supplementing Tonga side's of flavor treatment group; DG: Chinese angelica blood supplementing Tonga flavor side,
* *P＜0.001vs.Control, ###P＜0.001 vs.Diabetic vs.Diabetic; Abscissa: grouping, vertical coordinate: the acellular blood vessel in the unit are is counted Number/mm
2
Fig. 5 is the acellular vascular counts analysis of retina.In Fig. 5, DG: Chinese angelica blood supplementing Tonga flavor side,
* *P＜0.001 vs.Control, ###P＜0.001 vs.Diabetic vs.Diabetic; Abscissa: grouping, vertical coordinate: the acellular blood vessel in the unit are is counted Number/mm
2
Fig. 6 is that DG is to the inhibition of inflammatory Cytokines Expression in the diabetic retina.In Fig. 6, a is control, and b is Diabetic, and c is DG.DG: Chinese angelica blood supplementing Tonga flavor side,
*P＜0.05,
* *P＜0.001 vs.Control; #P＜0.05, ##P＜0.01, ###P＜0.001 vs.Diabetic; Abscissa: gene, mrna expression amount Normalized with β-acgtin that vertical coordinate :-actin proofreaies and correct.
The specific embodiment
The present invention is further illustrated in connection with accompanying drawing for following examples.
Embodiment 1:
1) Radix Astragali 25g, Radix Notoginseng 15g and Radix Angelicae Sinensis 20g are added water 350ml, boil after the immersion, get extracting solution; The time of described immersion is 20min, and described method of boiling can adopt microwave to boil the little 20min of boiling of rear little maintenance.
2) extracting solution is put into medicine storing pot, behind the diarrhea medicinal residues, be concentrated into every milliliter of medicinal liquid and be equivalent to the 1.1g crude drug, get the Chinese medicine that treatment of diabetic retinopathy becomes.Described concentrated method can adopt opens vacuum, condensed water and microwave and concentrates in extraction pot, but the Chinese medicine packing 10ml/ bottle that the gained treatment of diabetic retinopathy becomes.
Embodiment 2:
Similar to Example 1, its difference is that Radix Astragali 40g, Radix Notoginseng 10g and Radix Angelicae Sinensis 30g add water 300ml, boils after the immersion, gets extracting solution; The time of described immersion is 25min, and described method of boiling can adopt microwave to boil the little 30min of boiling of rear little maintenance.
Embodiment 3:
Similar to Example 1, its difference is that Radix Astragali 50g, Radix Notoginseng 25g and Radix Angelicae Sinensis 25g add water 400ml, boils after the immersion, gets extracting solution; The time of described immersion is 40min, and described method of boiling can adopt microwave to boil the little 25min of boiling of rear little maintenance.
Embodiment 4:
Similar to Example 1, its difference is that Radix Astragali 60g, Radix Notoginseng 40g and Radix Angelicae Sinensis 15g add water 500ml, boils after the immersion, gets extracting solution; The time of described immersion is 30min, and described method of boiling can adopt microwave to boil the little 40min of boiling of rear little maintenance.
Embodiment 5:
Similar to Example 1, its difference is that Radix Astragali 75g, Radix Notoginseng 30g and Radix Angelicae Sinensis 35g add water 400ml, boils after the immersion, gets extracting solution; The time of described immersion is 35min, and described method of boiling can adopt microwave to boil the little 35min of boiling of rear little maintenance.
Through tested studies show that of the attached First Hospital diabetes study of Xiamen University, spontaneous type 2 diabetes mellitus model Goto-Kakizaki(GK) the blood glucose compared with normal Wistar rat of rat significantly increases, when 10 monthly ages, obvious blood capillary seepage occurring increases, leukocyte adhesion increases in the number of blood vessel wall, and a large amount of acellular inaccessible blood capillaries occur.The Chinese medicine preparation that treatment of diabetic retinopathy of the present invention becomes is comprised of three flavor folk prescriptions (Radix Astragali, Radix Notoginseng, Radix Angelicae Sinensis), Main Function be invigorate blood circulation, blood stasis dispelling and collateral dredging, can effectively improve the above-mentioned pathological change of GK diabetic retinal tissue in rat.Trypan Blue shows, the retina seepage showed increased of GK rat, and DG can correct this phenomenon (Fig. 1) effectively.Long-term hyperglycemia causes the leukocyte adhesion rate to increase, leukocyte adhesion is piled up in the blood capillary, form little embolus, block blood capillary and discharge the various active material, increase the weight of microcirculation disturbance, cause that retinal capillary basement membrane thickened, blood-retina barrier are broken, blood capillary is inaccessible, DG can reduce the adhesion of leukocyte blood vessel wall in retina effectively, thereby reduces local leukocyte recruitment to blood vessel wall and damage blood-retina barrier (Fig. 2 and 3).The formation of pericyte's apoptosis, forfeiture and acellular blood vessel has damaged the integrity of blood-retina barrier, also further increases the weight of amphiblestroid ischemia, anoxia, advances the process of diabetic renal papillary necrosis.Thereby the formation of acellular blood vessel stoped generation and/or the progress (Figure 4 and 5) of DR when DG can reduce DR effectively.The improvement that DG changes retina pathology may be (Fig. 6) that the expression of intraretinal inflammatory factor realizes when suppressing diabetes.Above experimental result shows, amphiblestroid inflammatory reaction when this Chinese medicine preparation can suppress diabetes effectively reduces the formation of leukocyte adhesion, blood capillary seepage and acellular blood vessel, thereby prevents the generation of diabetic renal papillary necrosis or slow down its progress.
Claims (7)
1. the Chinese medicine that becomes of a treatment of diabetic retinopathy is characterized in that raw material consists of:
Wherein, the Radix Astragali, Radix Notoginseng and Radix Angelicae Sinensis are calculated in mass, and water is calculated by volume.
2. the Chinese medicine of a kind for the treatment of of diabetic retinopathy change as claimed in claim 1 is characterized in that the raw material of the Chinese medicine that described a kind for the treatment of of diabetic retinopathy becomes consists of Radix Astragali 2g, Radix Notoginseng 1g, Radix Angelicae Sinensis 1g, water 25 ~ 50ml; Wherein, the Radix Astragali, Radix Notoginseng and Radix Angelicae Sinensis are calculated in mass, and water is calculated by volume.
3. the preparation method of the Chinese medicine that becomes of a kind for the treatment of of diabetic retinopathy as claimed in claim 1 is characterized in that may further comprise the steps:
1) Radix Astragali, Radix Notoginseng and Radix Angelicae Sinensis are added water, boil after the immersion, get extracting solution;
2) extracting solution is put into medicine storing pot, behind the diarrhea medicinal residues, be concentrated into every milliliter of medicinal liquid and be equivalent to the 1.1g crude drug, get the Chinese medicine that treatment of diabetic retinopathy becomes.
4. the preparation method of the Chinese medicine of a kind for the treatment of of diabetic retinopathy change as claimed in claim 3 is characterized in that in step 1), and the time of described immersion is 20～40min.
5. the preparation method of the Chinese medicine of a kind for the treatment of of diabetic retinopathy change as claimed in claim 3 is characterized in that in step 1), and described method of boiling is to adopt microwave to boil the little 20～40min that boils of rear maintenance.
6. the preparation method of the Chinese medicine that becomes of a kind for the treatment of of diabetic retinopathy as claimed in claim 3 is characterized in that in step 2) in, described concentrated method is to adopt to open vacuum, condensed water and microwave concentrate in extraction pot.
7. the preparation method of the Chinese medicine of a kind for the treatment of of diabetic retinopathy change as claimed in claim 3 is characterized in that in step 2) in, the Chinese medicine dispensing 10ml/ bottle that the gained treatment of diabetic retinopathy becomes.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103830495A (en) * | 2014-02-25 | 2014-06-04 | 韩英 | Traditional Chinese medicine composition for treating fundus hemorrhage caused by diabetic retinopathy and preparation method thereof |
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| CN1419917A (en) * | 2001-11-21 | 2003-05-28 | 广州中医药大学第二临床医学院 | Oral pulse-invigorating liquid for treating chronic nephritis, and preparing method thereof |
| CN102631415A (en) * | 2011-03-01 | 2012-08-15 | 四川升和药业股份有限公司 | Traditional Chinese medicine composite as well as product and application thereof |
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Patent Citations (2)
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|---|---|---|---|---|
| CN1419917A (en) * | 2001-11-21 | 2003-05-28 | 广州中医药大学第二临床医学院 | Oral pulse-invigorating liquid for treating chronic nephritis, and preparing method thereof |
| CN102631415A (en) * | 2011-03-01 | 2012-08-15 | 四川升和药业股份有限公司 | Traditional Chinese medicine composite as well as product and application thereof |
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| > 20080229 唐爱华等 补阳还五汤化裁为主治疗单纯型糖尿病视网膜病变30例临床观察 32-33 1-7 第40卷, 第2期 * |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103830495A (en) * | 2014-02-25 | 2014-06-04 | 韩英 | Traditional Chinese medicine composition for treating fundus hemorrhage caused by diabetic retinopathy and preparation method thereof |
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Application publication date: 20130306 |