CN102942632A - Lactobacillus inoculation method for improving corn steeping effect - Google Patents
Lactobacillus inoculation method for improving corn steeping effect Download PDFInfo
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- CN102942632A CN102942632A CN2012104959028A CN201210495902A CN102942632A CN 102942632 A CN102942632 A CN 102942632A CN 2012104959028 A CN2012104959028 A CN 2012104959028A CN 201210495902 A CN201210495902 A CN 201210495902A CN 102942632 A CN102942632 A CN 102942632A
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Abstract
The invention relates to a lactobacillus inoculation method for improving a corn steeping effect, and belongs to the field of corn deep-processing. Old acid in a circulation impregnating tank group enters an added old acid tank by the aid of pressure difference and is placed in the added old acid tank for 7-9 hours, the temperature is 50 DEG C, the content of lactic acid reaches 2.2%-2.6%, the old acid cultivated in the added old acid tank is added to a steeping tank with the SO2 value ranging from 100ppm to 200ppm according to the weight ratio of 30:1 of steeping liquid to the cultivated old acid, and the liquid level of the old acid tank is kept unchanged. The content of the lactic acid in the steeping liquid is optimally increased in mass production, and the steeping effect and starch yield are improved.
Description
Technical field
The invention belongs to the corn deep processing field, refer in particular to a kind of improvement of corn soaking method.
Background technology
The immersion process of corn adopts continuous countercurrent circulation dipping flow process at present, steeping fluid adds impregnating autoclave against corn in impregnating autoclave, and continuous flow, in long impregnating autoclave of corn steeping time, quantitatively add dipping solution (new sulfurous acid solution), steeping fluid self is incubated circulation on one side in tank, in next tank, quantitatively carry on one side, guarantee in each tank liquid level; Remain that new sulfurous acid solution contacts with the corn that dipping time is grown (dipping closes to an end), and the corn that newly enters tank contacts with the old acid of discharge, thereby keep best dipping effect.Described old acid refers in immersion process the soak solution long with corn duration of contact, after soaking the steeping fluid dry matter content at 5%-9%, pH3-4, lactic acid content is after testing between 1.8%-2.0% in the old acid.
The shortcoming that above-mentioned immersion process exists is: lactic acid has a significant impact Maceration of Corn, can promote corn protein to soften and expansion, shortens dipping time, and lactic acid also can improve by reclaiming the horn shape endosperm starch yield of starch simultaneously.Steeping tank in the present circulated in countercurrent dipping flow process, working cycle is except adding eo-acid, pulp process China and foreign countries, all the time adverse current circulation immersion under the recycle pump effect.The best period of lactic acid effect is exactly before the adding eo-acid in immersion process, after adding eo-acid, along with soak solution constantly circulates, closes on that sulfurous acid concentration raises gradually in the tank, and the tank that sulfurous acid concentration is high suppresses the growth of lactic acid.The lactic acid amount of savings in period that is fit to growth milk-acid bacteria is inadequate, to add suppress lactic acid behind the eo-acid after, only have old sour tank lactic acid content just can reach about 2% in the circulation tank, other tank lactic acid contents all are lower than 2%, lactic acid content is on the low side in each tank, make soak time and soak effect to be affected, and then impact is loaded in large production raising and starch recoveries.
Summary of the invention
The invention provides a kind of lactobacillus inoculum method for improving the corn soaking effect, the lactic acid content after adding eo-acid that exists with the immersion process that solves present corn is low, and then the raising that impact is loaded in large production and the problem of starch recoveries.
The technical scheme that the present invention takes is: comprise the following steps:
(1) in existing corn steeping tank group, increase an old sour tank, this old sour tank is connected with each impregnating autoclave of existing corn steeping tank group by pipeline, valve and pump, make in the circulation impregnating autoclave group old acid enter this newly-increased old sour tank by pressure reduction, should old acid in this newly-increased old sour tank, place 7 ~ 9 hours, temperature 50 C, lactic acid content reaches 2.2% ~ 2.6%;
(2) detect the SO that soaks four hours each later steeping tanks
2Value is to SO
2The old acid of value after the steeping tank of 100ppm ~ 200ppm adds cultivation in the newly-increased old sour tank, according to steeping fluid: the part by weight of old acid=30:1 adding after cultivating, and keep old sour tank liquid level constant.
The present invention continues to improve its lactic acid content by old acid is independently cultivated, and inoculation is got back in the steeping fluid in proportion again, because SO2 concentration difference lactobacter growth speed are different, produces lactic acid production different.The experiment proved that the SO of steeping fluid
2Value is between 100ppm and 200ppm the time, and after the old acid of the present invention, lactic acid increases obviously after the inoculation, and lactic acid content on average can improve 0.06%.Steeping fluid after lactic acid increases like this is follow-up add eo-acid again after, although suppressed the lactic acid growth because in the accumulation that certain lactic acid production is arranged in earlier stage, can keep lactic acid content required in the circulation tank.Can improve 0.06% by lactic acid content in the steeping tank after this kind inoculation, guarantee the corn soaking effect.One be circulated throughout after, after the soaked corn fragmentation, starch content can reduce by 1% in the wheat bran, starch recoveries can improve 0.15%.
The present invention is by a large amount of simulation immersion tests, find the factor of restriction lactobacter growth, find by experiment the method that improves lactic acid in the steeping fluid, determine that finally the inoculation in large production adds best period and add-on, obtained improving the lactic acid content best approach in the soak solution in the large production, improved immersion effect and starch recoveries.
Description of drawings
Fig. 1 is lactic acid increment graph after the different SO2 content interval inoculations.
Embodiment
Embodiment 1
(1) in existing corn steeping tank group, increase an old sour tank, this old sour tank is connected with each impregnating autoclave of existing corn steeping tank group by pipeline, valve and pump, make in the circulation impregnating autoclave group old acid enter this newly-increased old sour tank by pressure reduction, should old acid in this newly-increased old sour tank, place 7 hours, temperature 50 C, lactic acid content reaches 2.2%;
(2) detect the SO that soaks four hours each later steeping tanks
2Value is to SO
2The old acid of value after the steeping tank of 100ppm ~ 200ppm adds cultivation in the newly-increased old sour tank, according to steeping fluid: the part by weight of old acid=30:1 adding after cultivating, and keep old sour tank liquid level constant.
Embodiment 2
(1) in existing corn steeping tank group, increase an old sour tank, this old sour tank is connected with each impregnating autoclave of existing corn steeping tank group by pipeline, valve and pump, make in the circulation impregnating autoclave group old acid enter this newly-increased old sour tank by pressure reduction, should old acid in this newly-increased old sour tank, place 8 hours, temperature 50 C, lactic acid content reaches 2.4%;
(2) detect the SO that soaks four hours each later steeping tanks
2Value is to SO
2The old acid of value after the steeping tank of 100ppm ~ 200ppm adds cultivation in the newly-increased old sour tank, according to steeping fluid: the part by weight of old acid=30:1 adding after cultivating, and keep old sour tank liquid level constant.
Embodiment 3
(1) in existing corn steeping tank group, increase an old sour tank, this old sour tank is connected with each impregnating autoclave of existing corn steeping tank group by pipeline, valve and pump, make in the circulation impregnating autoclave group old acid enter this newly-increased old sour tank by pressure reduction, should old acid in this newly-increased old sour tank, place 9 hours, temperature 50 C, lactic acid content reaches 2.6%;
(2) detect the SO that soaks four hours each later steeping tanks
2Value is to SO
2The old acid of value after the steeping tank of 100ppm ~ 200ppm adds cultivation in the newly-increased old sour tank, according to steeping fluid: the part by weight of old acid=30:1 adding after cultivating, and keep old sour tank liquid level constant.
Bottom further proves technical solution of the present invention by experiment.
The inventor proves no matter be by add carbon source in soak solution by experiment, improve the method for reducing sugar in the soak solution, or by in soak solution, adding nitrogenous source, increase the required nitrogenous source of lactobacter growth and trace element etc., from culture effect, fail obviously to improve lactic acid content in the soak solution.
And find that by the lactobacillus inoculum experiment effect is remarkable:
After getting soak solution in the old sour tank and cultivating for some time, according to steeping fluid: old sour inoculation liquid=30:1 joins different SO
2In the soak solution of concentration, 50 ℃ of constant temperature culture 7 ~ 9 hours, lactic acid content changes such as Fig. 1, as shown in Figure 1 before and after the contrast inoculation: at SO
2The rear lactic acid content of inoculation increases obviously in the steeping tank between value 100ppm and the 200ppm, can improve 0.06%.
Claims (1)
1. a lactobacillus inoculum method that is used for improving the corn soaking effect comprises the following steps:
(1) in existing corn steeping tank group, increase an old sour tank, this old sour tank is connected with each impregnating autoclave of existing corn steeping tank group by pipeline, valve and pump, make in the circulation impregnating autoclave group old acid enter this newly-increased old sour tank by pressure reduction, should old acid in this newly-increased old sour tank, place 7 ~ 9 hours, temperature 50 C, lactic acid content reaches 2.2% ~ 2.6%;
(2) detect the SO that soaks four hours each later steeping tanks
2Value is to SO
2The old acid of value after the steeping tank of 100ppm ~ 200ppm adds cultivation in the newly-increased old sour tank, according to steeping fluid: the part by weight of old acid=30:1 adding after cultivating, and keep old sour tank liquid level constant.
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Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109234184A (en) * | 2018-06-24 | 2019-01-18 | 山东省科学院生物研究所 | A kind of direct putting type microbial bacterial agent and its application in wet process corn soaking |
CN109913388A (en) * | 2019-03-20 | 2019-06-21 | 中粮集团有限公司 | Improve composite bacteria agent and its application of corn soaking effect |
CN110169537A (en) * | 2019-04-19 | 2019-08-27 | 安徽丰原发酵技术工程研究有限公司 | A kind of method of corn soaking new process preparation food-grade corn plumule |
CN110169488A (en) * | 2019-04-19 | 2019-08-27 | 安徽丰原发酵技术工程研究有限公司 | A kind of method of corn soaking new process preparation food-grade corn albumen |
CN110179054A (en) * | 2019-04-19 | 2019-08-30 | 安徽丰原发酵技术工程研究有限公司 | A kind of corn soaking new process |
CN110214956A (en) * | 2019-04-19 | 2019-09-10 | 安徽丰原发酵技术工程研究有限公司 | A kind of method of corn soaking new process preparation food-grade corn fiber |
CN114058669A (en) * | 2020-08-04 | 2022-02-18 | 山东福洋生物科技股份有限公司 | Method for evaluating corn soaking effect |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4086135A (en) * | 1973-12-14 | 1978-04-25 | Cpc International Inc. | Process for stepping grain and products obtained therefrom |
US4359528A (en) * | 1979-09-04 | 1982-11-16 | Roquette Freres | Corn steeping process for products thus obtained and their use in the manufacture of penicillin |
CN87101146A (en) * | 1986-12-15 | 1988-08-10 | Cpc国际公司 | Improved corn steep liquor |
CN1496994A (en) * | 2002-09-27 | 2004-05-19 | 玉米制品国际有限公司 | Method for reducing content of sugar in light corn extrcution |
CN1831013A (en) * | 2006-04-18 | 2006-09-13 | 吉林大学 | Corn starch soaking prodn. process of adding vegetable protease |
CN101293932A (en) * | 2008-04-17 | 2008-10-29 | 窦金玉 | Reversed flow slurry circulation immersion technique in corn starch process |
-
2012
- 2012-11-29 CN CN201210495902.8A patent/CN102942632B/en active Active
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4086135A (en) * | 1973-12-14 | 1978-04-25 | Cpc International Inc. | Process for stepping grain and products obtained therefrom |
US4359528A (en) * | 1979-09-04 | 1982-11-16 | Roquette Freres | Corn steeping process for products thus obtained and their use in the manufacture of penicillin |
CN87101146A (en) * | 1986-12-15 | 1988-08-10 | Cpc国际公司 | Improved corn steep liquor |
CN1496994A (en) * | 2002-09-27 | 2004-05-19 | 玉米制品国际有限公司 | Method for reducing content of sugar in light corn extrcution |
CN1831013A (en) * | 2006-04-18 | 2006-09-13 | 吉林大学 | Corn starch soaking prodn. process of adding vegetable protease |
CN101293932A (en) * | 2008-04-17 | 2008-10-29 | 窦金玉 | Reversed flow slurry circulation immersion technique in corn starch process |
Non-Patent Citations (3)
Title |
---|
DR. M. ROUSHDI,E TAL.: ""Factors Improving the Steeping Process of Corn Grains. Part III. Conditions Favouring Lactic Acid Formation During Corn Steeping and Its Effect"", 《STARCH》 * |
任海松等: ""玉米湿磨生产中减少浸泡时间的研究进展"", 《中国食物与营养》 * |
曹亚彬等: ""玉米浸渍过程中乳酸杆菌作用的研究"", 《生物技术》 * |
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109234184A (en) * | 2018-06-24 | 2019-01-18 | 山东省科学院生物研究所 | A kind of direct putting type microbial bacterial agent and its application in wet process corn soaking |
CN109234184B (en) * | 2018-06-24 | 2020-04-28 | 山东省科学院生物研究所 | Direct-throwing microbial agent and application thereof in wet corn soaking |
CN109913388A (en) * | 2019-03-20 | 2019-06-21 | 中粮集团有限公司 | Improve composite bacteria agent and its application of corn soaking effect |
CN110169537A (en) * | 2019-04-19 | 2019-08-27 | 安徽丰原发酵技术工程研究有限公司 | A kind of method of corn soaking new process preparation food-grade corn plumule |
CN110169488A (en) * | 2019-04-19 | 2019-08-27 | 安徽丰原发酵技术工程研究有限公司 | A kind of method of corn soaking new process preparation food-grade corn albumen |
CN110179054A (en) * | 2019-04-19 | 2019-08-30 | 安徽丰原发酵技术工程研究有限公司 | A kind of corn soaking new process |
CN110214956A (en) * | 2019-04-19 | 2019-09-10 | 安徽丰原发酵技术工程研究有限公司 | A kind of method of corn soaking new process preparation food-grade corn fiber |
CN114058669A (en) * | 2020-08-04 | 2022-02-18 | 山东福洋生物科技股份有限公司 | Method for evaluating corn soaking effect |
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