CN102928368A - Method for measuring homogeneous degree of fodder - Google Patents

Method for measuring homogeneous degree of fodder Download PDF

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Publication number
CN102928368A
CN102928368A CN2012104225448A CN201210422544A CN102928368A CN 102928368 A CN102928368 A CN 102928368A CN 2012104225448 A CN2012104225448 A CN 2012104225448A CN 201210422544 A CN201210422544 A CN 201210422544A CN 102928368 A CN102928368 A CN 102928368A
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methyl violet
tracer
feed
uniformity coefficient
sample
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CN102928368B (en
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付晓
王虎
周丽
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Sichuan New Hope Animal Husbandry Technology Co., Ltd.
New Hope Liuhe Co Ltd
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Sichuan New Hope Animal Husbandry Technology Co Ltd
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Abstract

The invention discloses a method for measuring the homogeneous degree of a fodder. The method comprises the following steps of: dissolving methyl violet by using ethanol with the volume percent concentration being more than 95% to prepare a methyl violet solution; mixing the prepared methyl violet solution with a tracer carrier for uniformly stirring and drying to obtain a methyl violet tracer; then adding the methyl violet tracer to the fodder for preparing a fodder sample; and measuring the homogeneous degree of the fodder sample. By utilizing the method, methyl violet solid does not need to be smashed, the method is environment-friendly, and health threat to operating workers is greatly reduced. Meanwhile, the methyl violet solid does not need to be smashed so that the method for preparing the methyl violet tracer is simple, convenient and easy to operate, investment in pulverizer equipment is also eliminated, and the production cost is reduced. Further more, a sampling device is adopted for sampling when the method is used for detecting the homogeneous degree of the samples, and the samples in different positions of a mixing machine can be sampled so that the condition of the homogeneous degree of the fodder can be truthfully reflected.

Description

A kind of method of measuring the feed uniformity coefficient
Technical field
The present invention relates to a kind of method of measuring uniformity coefficient, particularly a kind of method of measuring the feed uniformity coefficient.
Background technology
Methyl violet is commonly called as gentian violet, and crystal violet can be used as disinfection preservative, also as analytical reagent; But belong to medium malicious class, the diseases such as the visible Chronic gastrointestinal diseases of the workman of contact, laryngitis, rhinitis, dermatitis and conjunctivitis increase, but swallow Ulceration, and Long contact time can cause headache, nausea and vomiting.
In the production run of feed, the degree of uniformity that mixes in order to detect feed is mixed in tracer in the feed when feed mixes, and then feed is taken a sample, and reflects the feed mixture homogeneity by the content that detects tracer in each sample.Can carry out disinfection with anticorrosion to feed because a small amount of methyl violet is present in the feed, existing feed tracer adopts methyl violet usually.What existing methyl violet tracer adopted is the methyl violet powder, and for the tracer that makes small amount can be evenly distributed in the feed, then the particle diameter of methyl violet powder needed 200 mesh sieves.The preparation method of existing methyl violet tracer is simple, directly methyl violet is pulverized, and crosses 200 mesh sieves, namely gets the methyl violet tracer.Such methyl violet tracer preparation method, although simple, but because needs are pulverized virose methyl violet, sieve, can produce more methyl violet dust, environment has been caused larger pollution, and the operator works in the environment that the methyl violet dust is arranged for a long time, and workman's health has been caused great threat.In the prior art, when detecting the degree of uniformity of feed mixing, generally can only get the sample on feed exit or feed surface, not get the sample of feed inside.Such testing result can not be reacted the mixture homogeneity situation of same batch of feed really.
Summary of the invention
The object of the invention is to overcome existing above-mentioned deficiency in the prior art, a kind of method of measuring the feed uniformity coefficient is provided, the method has reduced the pollution of methyl violet dust to environment, has improved the detection accuracy of feed mixture homogeneity.
Further purpose of the present invention provides a kind of method that adopts the measurement feed uniformity coefficient that sampler takes a sample, and the method more can be reacted the situation of feed uniformity coefficient really.
In order to realize the foregoing invention purpose, the invention provides following technical scheme:
A kind of method of measuring the feed uniformity coefficient may further comprise the steps:
(1), preparation methyl violet tracer
(1) is 95% above ethanol dissolving methyl violet with concentration expressed in percentage by volume, makes methyl violet solution;
(2) the methyl violet solution that step (1) is made mixes with the tracer carrier, stirs;
(3) potpourri of drying steps (2) methyl violet solution and tracer carrier gets the methyl violet tracer;
(2), preparation Feed Sample
After feed added mixer, again the methyl violet tracer is added mixer, the quality of methyl violet tracer is 0.5～2:1000 with the ratio of feeding quality, mixes;
(3), cloud test
Sampling with the content of methyl violet in the colorimetric method for determining sample, is reacted the mixture homogeneity of feed with the difference of methyl violet content in the sample of diverse location in the above-mentioned feed.
As mentioned above, in the preparation of methyl violet tracer, the quality of methyl violet and ethanol and volume ratio are 1g:10-20mL; Filter after the ethanol dissolving methyl violet, make methyl violet solution; The quality of tracer carrier and ethanol and volume ratio are 5-10g:1mL; The tracer carrier is a kind of in calcium monohydrogen phosphate, inferior powder, the rice bran; The particle diameter of described tracer carrier is the 60-100 order.
The inventor finds in long-term practical study, always there is part not to be dissolved in the existing methyl violet raw material and extracts with the existence of the impurity in the organic solvent, and impurity content can't be predicted and skewness, so that can not reflect more exactly the feed mixture homogeneity by methyl violet content in the working sample, thereby the accuracy that the feed mixture homogeneity is detected is relatively poor.Can not be dissolved in the shortcoming of extracting the impurity in the solvent in order to overcome to contain in the methyl violet raw material, make the methyl violet tracer of preparation can reflect more exactly the feed mixing uniformity, step in the said method (1) is preferably filtered after ethanol dissolving methyl violet, makes methyl violet solution.
As mentioned above, adopt the sampler sampling when taking a sample in the step (three), described sampler, comprise sampler barrel, be provided with elongated slot and baffle plate on the described sampler barrel, described baffle plate is arranged on wherein bus of described sampler barrel, described bus and described elongated slot stagger, rotate in the described sampler barrel and be provided with capping, described capping one end is fixedly connected with handle, and described handle passes described sampler barrel bottom and stretches out sampler barrel; The section at the bus place of described baffle plate and described sampler barrel is perpendicular; Described sampler barrel top is coniform.
The present invention adopts the sampler sampling when the test sample uniformity coefficient, can obtain the sample of diverse location in the mixer, can react more really the situation of feed uniformity coefficient, has improved the detection accuracy of feed mixture homogeneity.
Compared with prior art, beneficial effect of the present invention:
The inventive method adopts dissolving methyl violet solid, mix with the tracer carrier, the potpourri of dry methyl violet solution and tracer carrier again, finally make the methyl violet tracer, so that the inventive method need not to pulverize the methyl violet solid, environmentally friendly, reduced largely the health threat to the operator; Simultaneously, need not the methyl violet solid is pulverized, not only make the method for preparing the methyl violet tracer become more simple and easy to operate, but also saved the input of comminutor equipment, reduced production cost.Further, the inventive method is filtered behind dissolving methyl violet solid, has removed and has not allowed impurity, when making the methyl violet tracer of preparation be used for the feed saturation degree to detect, can improve the accuracy of testing result.Further, the present invention adopts the sampler sampling when the test sample uniformity coefficient, can obtain the sample of mixer inside, can react more really the situation of feed uniformity coefficient.
Description of drawings
Fig. 1 is the structural representation of sampler among the present invention;
Mark among the figure: 1-sampler barrel, 2-capping, 3-handle, 4-baffle plate.
Embodiment
The present invention is described in further detail below in conjunction with test example and embodiment.But this should be interpreted as that the scope of the above-mentioned theme of the present invention only limits to following embodiment, all technology that realizes based on content of the present invention all belong to scope of the present invention.
Embodiment 1
A kind of method of measuring the feed uniformity coefficient may further comprise the steps:
(1), preparation methyl violet tracer
(1) is the methyl violet of 95% ethanol 200mL dissolving 10g with concentration expressed in percentage by volume, makes methyl violet solution;
(2) the methyl violet solution that step (1) is made mixes with the 2000g calcium monohydrogen phosphate, stirs, and the particle diameter of described calcium monohydrogen phosphate is 100 orders;
(3) put drying with the potpourri of step (2) methyl violet solution and tracer carrier is spacious, make the alcohol solvent volatilization, obtain the methyl violet tracer.
(2), preparation Feed Sample
Behind 1t feed adding mixer, again 2kg methyl violet tracer is added mixer, mix.
(3), cloud test
Adopt sampler to gather the sample of diverse location in the mixer, with the content of methyl violet in the colorimetric method for determining sample, react the mixture homogeneity of feed with the difference of methyl violet content in the sample of diverse location in the above-mentioned feed.
Embodiment 2-4
(1), the method for preparing the methyl violet tracer
Prepare the methyl violet tracer by embodiment 1 described step, compare with embodiment 1, partial parameters related in the method is different, and is as shown in table 1, without specifying, all identical with embodiment 1.
Table 1
Figure BDA00002327368000051
(2), preparation Feed Sample
After feed added mixer, again the methyl violet tracer is added mixer, mix.
It is as shown in the table for concrete addition:
Table 2
(3), cloud test
Adopt sampler to gather the sample of diverse location in the mixer, with the content of methyl violet in the colorimetric method for determining sample, react the mixture homogeneity of feed with the difference of methyl violet content in the sample of diverse location in the above-mentioned feed.
The methyl violet tracer that embodiment 1-4 is prepared, concrete operations are as follows:
The Feed Sample that takes by weighing 10.00g ± 0.05g is placed in the small beaker of 100mL, add 30mL absolute ethyl alcohol, stirring and dissolving methyl tracer, beaker loam cake one surface plate filters with the middling speed qualitative filter paper behind the 30min.Make blank with absolute ethyl alcohol and regulate zero point, use spectrophotometer, under 590 wavelength, measure the absorbance of filtrate with the 5mm cuvette.
The absorbance that records take 10 samples of same embodiment is as X1, X2, X3, X4, X5, X6, X7, X8, X9, X10, by formula 1., 2., 3. calculating mean value respectively
Figure BDA00002327368000061
Standard deviation S, coefficient of variation CV value (%).
X ‾ = X 1 + X 2 + X 3 + . . . + X 10 10 ……①
S = ( X 1 - X ‾ ) 2 + ( X 2 - X ‾ ) 2 + ( X 3 - X ‾ ) 2 + . . . + ( X 10 - X ‾ ) 2 10 - 1 ……②
CV = S X ‾ × 100 ……③
The absorbance that table 3, each sample record
Absorbance Embodiment 1 Embodiment 2 Embodiment 3 Embodiment 4
X1 0.403 0.433 0.403 0.423
X2 0.388 0.387 0.388 0.377
X3 0.443 0.413 0.443 0.363
X4 0.385 0.425 0.382 0.405
X5 0.419 0.399 0.409 0.399
X6 0.431 0.401 0.434 0.411
X7 0.409 0.425 0.405 0.386
X8 0.364 0.395 0.368 0.415
X9 0.412 0.372 0.422 0.392
X10 0.391 0.431 0.381 0.435
The accuracy of table 4, each embodiment
Figure BDA00002327368000071
Use the methyl violet tracer of embodiment 1-4 preparation, because in the preparation, the impurity of not allowing in the methyl violet raw material is removed, so that the methyl violet tracer of embodiment 1-4 preparation, be used for the test of feed mixture homogeneity, improved the detection accuracy of feed mixture homogeneity.Further, the present invention adopts the sampler sampling when the test sample uniformity coefficient, can obtain the sample of diverse location in the mixer, can react more really the situation of feed uniformity coefficient.
Embodiment 5
A kind of method of measuring the feed uniformity coefficient may further comprise the steps:
(1), prepare the method for methyl violet tracer, may further comprise the steps:
(1) is the methyl violet of 95% ethanol 150mL dissolving 10g with concentration expressed in percentage by volume, filters, make methyl violet solution;
(2) the methyl violet solution that step (1) is made mixes with the 1500g calcium monohydrogen phosphate, stirs, and the particle diameter of described calcium monohydrogen phosphate is 65 orders;
(3) put drying with the potpourri of step (2) methyl violet solution and tracer carrier is spacious, make the alcohol solvent volatilization, obtain the methyl violet tracer.
(2), preparation Feed Sample
Behind 1t feed adding mixer, again 1.5kg methyl violet tracer is added mixer, mix.
(3), cloud test
Adopt sampler to gather the sample of diverse location in the mixer, with the content of methyl violet in the colorimetric method for determining sample, react the mixture homogeneity of feed with the difference of methyl violet content in the sample of diverse location in the above-mentioned feed.
Embodiment 6-7
(1), embodiment 6-7 prepares the method for methyl violet tracer
Prepare the methyl violet tracer by embodiment 5 described steps, compare with embodiment 5, partial parameters related in the method is different, and is as shown in table 4, without specifying, all identical with embodiment 5.
Table 5
Figure BDA00002327368000081
(2), preparation Feed Sample
After feed added mixer, again the methyl violet tracer is added mixer, mix.Concrete addition is as shown in the table:
Table 6
Figure BDA00002327368000082
(3), cloud test
Adopt sampler to gather the sample of diverse location in the mixer, with the content of methyl violet in the colorimetric method for determining sample, react the mixture homogeneity of feed with the difference of methyl violet content in the sample of diverse location in the above-mentioned feed.
The methyl violet tracer that embodiment 6-7 is prepared and the methyl violet tracer that does not prepare through the present invention are used for the mensuration of feed uniformity coefficient, and concrete operations are as follows:
The Feed Sample that takes by weighing 10.00g ± 0.05g is placed in the small beaker of 100mL, add 30mL absolute ethyl alcohol, stirring and dissolving methyl tracer, beaker loam cake one surface plate filters with the middling speed qualitative filter paper behind the 30min.Make blank with absolute ethyl alcohol and regulate zero point, use spectrophotometer, under 590 wavelength, measure the absorbance of filtrate with the 5mm cuvette.
The absorbance that records take 10 samples of same embodiment is as X1, X2, X3, X4, X5, X6, X7, X8, X9, X10, by formula 1., 2., 3. calculating mean value respectively
Figure BDA00002327368000091
Standard deviation S, coefficient of variation CV value (%).
X ‾ = X 1 + X 2 + X 3 + . . . + X 10 10 ……①
S = ( X 1 - X ‾ ) 2 + ( X 2 - X ‾ ) 2 + ( X 3 - X ‾ ) 2 + . . . + ( X 10 - X ‾ ) 2 10 - 1 ……②
CV = S X ‾ × 100 ……③
The absorbance that table 7, each sample record
Absorbance Embodiment 5 Embodiment 6 Embodiment 7
X1 0.413 0.403 0.403
X2 0.397 0.458 0.388
X3 0.423 0.423 0.423
X4 0.425 0.385 0.385
X5 0.459 0.419 0.419
X6 0.461 0.431 0.421
X7 0.436 0.467 0.379
X8 0.384 0.405 0.464
X9 0.392 0.412 0.412
X10 0.411 0.391 0.391
The accuracy of table 8, each embodiment
Figure BDA00002327368000101
Use the methyl violet tracer of embodiment 6-7 preparation, because in the preparation, the impurity of not allowing in the methyl violet raw material is removed, so that the methyl violet tracer of embodiment 6-7 preparation, be used for the test of feed mixture homogeneity, improved the detection accuracy of feed mixture homogeneity.Further, the present invention adopts the sampler sampling when the test sample uniformity coefficient, can obtain the sample of diverse location in the mixer, can react more really the situation of feed uniformity coefficient.

Claims (9)

1. method of measuring the feed uniformity coefficient is characterized in that may further comprise the steps:
(1), preparation methyl violet tracer
(1) is 95% above ethanol dissolving methyl violet with concentration expressed in percentage by volume, makes methyl violet solution;
(2) the methyl violet solution that step (1) is made mixes with the tracer carrier, stirs;
(3) potpourri of drying steps (2) methyl violet solution and tracer carrier gets the methyl violet tracer;
(2), preparation Feed Sample
After feed added mixer, again the methyl violet tracer is added mixer, the quality of methyl violet tracer is 0.5～2:1000 with the ratio of feeding quality, mixes;
(3), cloud test
Sampling with the content of methyl violet in the colorimetric method for determining sample, is reacted the mixture homogeneity of feed with the difference of methyl violet content in the sample of diverse location in the above-mentioned feed.
2. the method for measurement feed uniformity coefficient as claimed in claim 1 is characterized in that in the preparation of methyl violet tracer, and the quality of methyl violet and ethanol and volume ratio are 1g:10-20mL.
3. the method for measurement feed uniformity coefficient as claimed in claim 1 is characterized in that in the preparation of methyl violet tracer, filters after the described ethanol dissolving methyl violet, makes methyl violet solution.
4. the method for measurement feed uniformity coefficient as claimed in claim 1 is characterized in that in the preparation of methyl violet tracer, and the quality of tracer carrier and ethanol and volume ratio are 5-10 g:1mL.
5. the method for measurement feed uniformity coefficient as claimed in claim 1 is characterized in that in the preparation of methyl violet tracer, and the tracer carrier is a kind of in calcium monohydrogen phosphate, inferior powder, the rice bran.
6. the method for measurement feed uniformity coefficient as claimed in claim 1 is characterized in that in the preparation of methyl violet tracer, and the particle diameter of described tracer carrier is the 60-100 order.
7. the method for measurement feed uniformity coefficient as claimed in claim 1, it is characterized in that: adopt the sampler sampling when taking a sample in the step (three), described sampler, comprise sampler barrel, be provided with elongated slot and baffle plate on the described sampler barrel, described baffle plate is arranged on wherein bus of described sampler barrel, described bus and described elongated slot stagger, rotate in the described sampler barrel and be provided with capping, described capping one end is fixedly connected with handle, and described handle passes described sampler barrel bottom and stretches out sampler barrel.
8. the method for measurement feed uniformity coefficient as claimed in claim 7 is characterized in that, the section at the bus place of described baffle plate and described sampler barrel is perpendicular.
9. the method for the measurement feed uniformity coefficient described in claim 7 or 8 is characterized in that, described sampler barrel top is coniform.
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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105067539A (en) * 2015-07-16 2015-11-18 浙江大学 Method for detecting mixing uniformity of soil heavy metal pollution samples
CN106404701A (en) * 2016-08-31 2017-02-15 上海交通大学 Method for rapidly detecting mixing uniformity of protein in polymer solution
CN108072740A (en) * 2016-11-10 2018-05-25 内蒙古伊利实业集团股份有限公司 A kind of assay method of the mixture homogeneity of dry mixed powder product
CN108072654A (en) * 2017-12-29 2018-05-25 山东海奥斯生物科技有限公司 Judge the method that collagen auxiliary material and auxiliary material are uniformly mixed with major ingredient
CN109211731A (en) * 2018-10-11 2019-01-15 苏州优霹耐磨复合材料有限公司 The rapid detection method of flux-cored wire material mixing uniformity
CN110376046A (en) * 2019-08-07 2019-10-25 北京大学人民医院(北京大学第二临床医学院) Mouse Nerve retrograde tracing marks component
CN111239070A (en) * 2020-02-12 2020-06-05 首钢集团有限公司 Method for measuring uniform mixing degree of raw materials of pellet mixer
CN115155432A (en) * 2022-08-22 2022-10-11 陕西山秦能源技术服务有限公司 Preparation process and equipment of reservoir transformation agent

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US4421858A (en) * 1981-07-10 1983-12-20 Cambridge Products, Ltd. Method for determining the concentration of selected ingredients in animal feeds
CN201464223U (en) * 2009-07-01 2010-05-12 天津美德太平洋科技有限公司 Disposable fully-sealed body fluid specimen collector
CN202837017U (en) * 2012-08-27 2013-03-27 四川新希望畜牧科技有限公司 Sampler

Patent Citations (3)

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US4421858A (en) * 1981-07-10 1983-12-20 Cambridge Products, Ltd. Method for determining the concentration of selected ingredients in animal feeds
CN201464223U (en) * 2009-07-01 2010-05-12 天津美德太平洋科技有限公司 Disposable fully-sealed body fluid specimen collector
CN202837017U (en) * 2012-08-27 2013-03-27 四川新希望畜牧科技有限公司 Sampler

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105067539A (en) * 2015-07-16 2015-11-18 浙江大学 Method for detecting mixing uniformity of soil heavy metal pollution samples
CN106404701A (en) * 2016-08-31 2017-02-15 上海交通大学 Method for rapidly detecting mixing uniformity of protein in polymer solution
CN108072740A (en) * 2016-11-10 2018-05-25 内蒙古伊利实业集团股份有限公司 A kind of assay method of the mixture homogeneity of dry mixed powder product
CN108072740B (en) * 2016-11-10 2021-08-27 内蒙古伊利实业集团股份有限公司 Method for measuring mixing uniformity of dry-method mixed powder product
CN108072654A (en) * 2017-12-29 2018-05-25 山东海奥斯生物科技有限公司 Judge the method that collagen auxiliary material and auxiliary material are uniformly mixed with major ingredient
CN108072654B (en) * 2017-12-29 2020-10-16 山东海奥斯生物科技有限公司 Method for judging collagen auxiliary material and uniform mixing of auxiliary material and main material
CN109211731A (en) * 2018-10-11 2019-01-15 苏州优霹耐磨复合材料有限公司 The rapid detection method of flux-cored wire material mixing uniformity
CN110376046A (en) * 2019-08-07 2019-10-25 北京大学人民医院(北京大学第二临床医学院) Mouse Nerve retrograde tracing marks component
CN111239070A (en) * 2020-02-12 2020-06-05 首钢集团有限公司 Method for measuring uniform mixing degree of raw materials of pellet mixer
CN111239070B (en) * 2020-02-12 2023-04-07 首钢集团有限公司 Method for measuring uniform mixing degree of raw materials of pellet mixer
CN115155432A (en) * 2022-08-22 2022-10-11 陕西山秦能源技术服务有限公司 Preparation process and equipment of reservoir transformation agent

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