CN104407070A - Method for detecting content of azodicarbonamide in food - Google Patents
Method for detecting content of azodicarbonamide in food Download PDFInfo
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Abstract
The invention discloses a method for detecting the content of azodicarbonamide in food. The method comprises the following steps: (1) weighing a certain amount of food, and adding dimethyl formamide for dissolution and filtration to obtain to-be-detected liquid; (2) determining the content of the azodicarbonamide in the to-be-detected liquid by high performance liquid chromatography, wherein a phosphoric acid water solution of which the pH equals to 3 is used as a mobile phase, and a photoelectric diode array detector is adopted. The method is easy to operate, low in detection limit, high in extraction efficiency, high in repeatability and low in cost, and requirements of laws and regulations on management and control of the azodicarbonamide are completely met.
Description
Technical field
The present invention relates to the detection field of food additives, particularly relate to the detection method of azobisformamide content in food.
Background technology
In February, 2014, U.S. CNN exposes fast food giant " Subway " (subway) in bread, adds azobisformamide event, and food-safety problem causes the concern of the public again.
Azobisformamide (Azodicarbonamide), has another name called azodicarbonamide, is a kind of food additives, there is bleaching and oxidation double action, be a kind of quick-acting flour gluten fortifier, can muscle brightened to flour and promote maturation, in order to improve baking goods quality.But there are some researches show in recent years, although azobisformamide itself is not carcinogenic, but it is in pyrolysis process, carcinogenic substance semicarbazides may be produced, and itself excessive amount also there will be asthma and irritated waits bad reaction, azobisformamide also can destroy vitamin in flour, affect calcareous absorption simultaneously.
China expressly provides in " GB2760-2011 food additives use standard ", and azobisformamide is as Flour ingredient, and maximum permission content is 0.045g/kg; The U.S. requires also to be 0.045g/kg to its limit value; The interpolation azobisformamide in food has been forbidden on the ground such as European Union, Australia, New Zealand, Singapore and Japan.In food, the test of azobisformamide has boundless test prospect, but be insoluble to cold water because azobisformamide has, be insoluble to the features such as alcohol, benzene, acetone and other organic solvent and high temperature easily decompose, in current food, the test of azobisformamide is also without any standard method of test, and periodical, magazine etc. do not have document to do relevant report yet.
Summary of the invention
Based on this, the invention provides the detection method of azobisformamide content in a kind of food, this method have simple to operate, extraction efficiency is high, detection limit is low, reproducible and low cost and other advantages.
In food, a detection method for azobisformamide content, comprises the steps:
(1) take a certain amount of food, add dimethyl formamide and dissolve, filter, obtain liquid to be measured;
(2) with the content of azobisformamide in high effective liquid chromatography for measuring liquid to be measured, with the phosphate aqueous solution of pH=3 for mobile phase, photodiode array detector is adopted.
Wherein in an embodiment, described a certain amount of food, is less than 2g.
Wherein in an embodiment, further comprising the steps of before described step (1): food is pulverized.
Wherein in an embodiment, described food is crushed to its maximum particle diameter and is less than 1mm
Wherein in an embodiment, organic filter membrane in described step (1), is adopted to carry out described filter operation.
Wherein in an embodiment, the specification of described organic filter membrane is 0.45 μm.
Wherein in an embodiment, the described mobile phase in described step (2) is placed in ultrasonic cleaner ultrasonic through 0.45 μm of organic membrane filtration before use.
Wherein in an embodiment, the described ultrasonic time is 10min ~ 15min.
Wherein in an embodiment, the described ultrasonic time is 10min.
Wherein in an embodiment, C18 chromatographic column selected by described high performance liquid chromatography, and described chromatographic column internal diameter is 250mm*4.6mm, and particle diameter is 5 μm.
In above-mentioned food, the detection method of azobisformamide content is relative to prior art, has following remarkable advantage:
(1) the present invention utilizes dimethyl formamide as extraction solvent, efficiently solves the deliquescent problem of azobisformamide, can extract the azobisformamide in food efficiently.
(2) the present invention utilizes high-efficient liquid phase color bands of a spectrum diode array detector to detect azobisformamide in liquid to be measured, solves the labile problem of azobisformamide high temperature.
(3) detection limit of the present invention is low, and extraction efficiency is high, reproducible, and cost is low, can meet the requirement of laws and regulations to azobisformamide management and control completely.
(4) the inventive method usable range is wide, is applicable to detect the food such as flour, bread and biscuit, and the preparation of the inventive method sample is simple, convenient and swift, is suitable for standardization.
Accompanying drawing explanation
Fig. 1 is the uv absorption spectra of azobisformamide, and in this spectrogram, ordinate represents percentage transmitance (T%), and horizontal ordinate represents wavelength, and unit is nm;
Fig. 2 is the chromatogram of 10mg/L azobisformamide standard items, and the ordinate in this chromatogram represents peak intensity, and horizontal ordinate represents retention time, and unit is min;
Fig. 3 is the canonical plotting of azobisformamide, and the ordinate in this curve map represents the response of gas chromatography peak area, and horizontal ordinate represents the concentration of azobisformamide.
Embodiment
For feature of the present invention, technological means and the specific purposes reached, function can be understood further, below in conjunction with embodiment, the present invention is described in further detail.
The preparation of liquid to be measured: precision takes food 1g, is accurate to 0.0001g, is placed in reaction bulb, add 8mL ~ 15mL dimethyl formamide, mechanical jolting 20min ~ 40min, leave standstill 5min ~ 15min, treat layering, get supernatant liquor 0.45 μm of organic membrane filtration, obtain liquid to be measured.
It should be noted that, if add dimethyl formamide jolting, after leaving standstill, without obvious layering, then use hydro-extractor to carry out centrifugal to sample under 1000r/min rotating speed, get supernatant liquor after centrifugal and cross 0.45 μm of organic membrane filtration.
Detecting instrument is thermoelectricity Ultimate 3000 high performance liquid chromatograph band diode array detector or equivalent apparatus;
Chromatographic condition is as follows:
Chromatographic column: Dionex Acclaim 200 C18 chromatographic column, its internal diameter is 250mm*4.6mm, and particle diameter is 5 μm or equal performance chromatographic columns;
Sampling volume: 10 μ L;
Mobile phase: phosphate aqueous solution, preferably, pH=3;
Column flow rate: 1mL/min;
Column temperature: 30 DEG C;
Determined wavelength: 245nm;
Sweep limit: 200nm ~ 600nm.
Be appreciated that, cold water is insoluble to because azobisformamide has, be insoluble to alcohol, benzene, the majority of organic solvents such as acetone, be dissolved completely in extractant in order to ensure the azobisformamide in sample, the present invention tests azobisformamide at methyl alcohol, acetonitrile, acetone, tetrahydrofuran, methylene chloride, sherwood oil, ethyl acetate, benzene, solubleness in a series of organic solvent such as dimethyl formamide, proved by many experiments, azobisformamide has good solubleness in dimethyl formamide, 11g/L can be reached, thus can dissolve the azobisformamide added in food.In addition, azobisformamide only needs jolting namely can dissolve in dimethyl formamide, can simplify the processing procedure of sample like this, promotes the extraction efficiency of azobisformamide, reduces testing cost.
Preferably, when adding dimethyl formamide dissolving, meanwhile, be heated to 40 to 50 degrees Celsius, vibrate 2 to 5 minutes at such a temperature.Preferably, after being heated to 40 to 50 degrees Celsius, sonic oscillation 2 to 5 minutes.Like this, the dissolution time of azobisformamide can be shortened, promote the extraction efficiency of azobisformamide.
In order to make azobisformamide have good separating effect on a column, the selection of suitable mobile phase is the emphasis that azobisformamide is applied on high performance liquid chromatography.The present invention selects pH=3 phosphate aqueous solution to be mobile phase, because under room temperature condition, azobisformamide is slightly soluble in water, its solubleness is 35mg/L, and azobisformamide in alkalescent, can increase azobisformamide solubleness at mobile phase by the acidity increasing mobile phase owing to having two amide groups.
Proved by many experiments, when selection pH=3 phosphate aqueous solution is mobile phase, when column flow rate is 1mL/min, azobisformamide can be realized there is good separating effect.As shown in Figure 2, it is dissolved in the chromatogram in dimethyl formamide for azobisformamide standard model.From chromatogram, the retention time of azobisformamide is 4.383min, and the retention time of solvent dimethylformamide is 8.970min, azobisformamide can be separated effectively with solvent dimethylformamide from high performance liquid chromatography, thus avoid the interference of solvent peak.
For ensureing the accuracy of result further, wherein, the phosphate aqueous solution of pH=3 puts into the ultrasonic 10min ~ 15min of ultrasonic cleaner before use after the organic membrane filter of 0.45 μm, fully deviates from the gas in the phosphate aqueous solution of pH=3.
The selection of determined wavelength: after azobisformamide dimethyl formamide is configured to certain density solution, scan under diode array, with wavelength (nm) for horizontal ordinate, percentage transmitance (T%) draws out azobisformamide uv absorption spectra for ordinate, the results are shown in Figure 1.As can be seen from Figure 1, azobisformamide has absorption maximum at 245nm place, and therefore, selection 245nm is determined wavelength.
Be specific embodiment part below.
The drafting of embodiment 1 standard working curve
Azobisformamide standard items (purity is 97%) 0.0512g is accurately taken in the volumetric flask of 25mL with electronic balance, dissolve with dimethyl formamide and be settled to scale mark, shake up, be mixed with the azobisformamide stock solution of 1986.56mg/L.Get 503 μ L storing solutions in 10mL volumetric flask, be settled to scale mark with dimethyl formamide, obtain 100mg/L azobisformamide intermediate solution.Get in 0.1mL, 0.2mL, 0.5mL, 1mL, 2mL intermediate solution to 5 10mL volumetric flask respectively, be settled to scale mark with dimethyl formamide, obtain the standard working solution of 1.0mg/L, 2.0mg/L, 5.0mg/L, 10.0mg/L, 20.0mg/L totally 5 points.Pipette 10 μ L and inject high performance liquid chromatograph, measure by chromatographic condition, with the peak area of chromatographic peak for ordinate, the concentration corresponding with it is horizontal ordinate mapping, drawing standard working curve.
The typical curve obtained is Y=1.198X+0.365, the linear R of typical curve
2=0.9997, linearly well, can be used for the quantitative detection of azobisformamide.
The detection of azobisformamide content in embodiment 2 flour
Prepare liquid to be measured: precision takes flour 1g (being accurate to 0.0001g), is placed in 40mL reaction bulb, adds 10mL dimethyl formamide, machinery jolting 30min, leaves standstill 10min, layering, get supernatant liquor 0.45 μm of organic membrane filtration, obtain liquid to be measured.
Repeat the preparation process 3 times of above-mentioned liquid to be measured, obtain Duplicate Samples A, B, C of 3 parts of flour liquid to be measured.
With high performance liquid chromatography, above-mentioned liquid to be measured is analyzed, detecting instrument and chromatographic condition in the same manner as in Example 1, the concentration of liquid to be measured is drawn according to the typical curve in the peak area of corresponding chromatographic peak and embodiment 1, thus calculating the content (mg/kg) of azobisformamide in flour, mean value and relative standard deviation RSD further, testing result is in table 1.
Table 1
To flour Duplicate Samples A replication 5 times, test result is in table 2.
Table 2
The detection of azobisformamide content in embodiment 3 bread
Prepare liquid to be measured: after bread grinding, precision takes 1g (being accurate to 0.0001g), is placed in 40mL reaction bulb, add 10mL dimethyl formamide, mechanical jolting 30min, leave standstill 15min, layering, gets supernatant liquor 0.45 μm of organic membrane filtration, obtains liquid to be measured.
Repeat the preparation process 3 times of above-mentioned liquid to be measured, obtain Duplicate Samples A, B, C of 3 parts of bread liquid to be measured.
With high performance liquid chromatography, above-mentioned liquid to be measured is analyzed, detecting instrument and chromatographic condition in the same manner as in Example 1, the concentration treating sample measuring liquid is drawn according to the typical curve in the peak area of corresponding chromatographic peak and embodiment 1, thus calculating the content (mg/kg) of azobisformamide in bread, mean value and relative standard deviation RSD further, testing result is in table 3.
Table 3
To bread Duplicate Samples A replication 5 times, test result is in table 4.
Table 4
The detection of azobisformamide content in embodiment 4 biscuit
Prepare liquid to be measured: after being pulverized by biscuit, precision takes 1g (being accurate to 0.0001g), be placed in 40mL reaction bulb, add 10mL dimethyl formamide, machinery jolting 30min, use hydro-extractor to carry out centrifugal to sample under 1000r/min rotating speed, get supernatant liquor after centrifugal and cross 0.45 μm of organic membrane filtration, obtain liquid to be measured.
Repeat the preparation process 3 times of above-mentioned liquid to be measured, obtain Duplicate Samples A, B, C of 3 parts of biscuit liquid to be measured.
With high performance liquid chromatography, above-mentioned liquid to be measured is analyzed, detecting instrument and chromatographic condition in the same manner as in Example 1, the concentration treating sample measuring liquid is drawn according to the typical curve in the peak area of corresponding chromatographic peak and embodiment 1, thus calculating the content (mg/kg) of azobisformamide in biscuit, mean value, the recovery and relative standard deviation RSD further, testing result is in table 5.
Table 5
To biscuit Duplicate Samples A replication 5 times, test result is in table 6.
Table 6
Embodiment 5 recovery is tested
Standard solution in embodiment 1 is joined in the liquid to be measured of ready known negative result (after tested not containing azobisformamide), test according to embodiment 1 Instrumental analyzing detecting method.Do 6 horizontal surveies to this liquid to be measured to average, according to actual addition and measured result, calculate the recovery of standard addition of this liquid to be measured, test result is in table 7.
Table 7
The calculating of method detection limit: calculate instrument detection limit with 3 of noise times, on the basis of instrument detection limit, multiple increases the concentration of azobisformamide, when the signal to noise ratio (S/N ratio) (S/N) of target peak reaches 5, the concentration of azobisformamide is 7.5mg/kg, and namely method detects and is limited to 7.5mg/kg.
Data from table 1 to table 3 are known, and this method is to the detection method of azobisformamide content in flour, bread and biscuit, and method detects and is limited to 7.5mg/kg, and the recovery is between 91.5% ~ 98.5%, and RSD is between 1.8% ~ 3.9%.Visible, in above-mentioned food, the detection method of azobisformamide content has higher sensitivity, accuracy and precision, good reappearance and stability.
Each technical characteristic of the above embodiment can combine arbitrarily, for making description succinct, the all possible combination of each technical characteristic in above-described embodiment is not all described, but, as long as the combination of these technical characteristics does not exist contradiction, be all considered to be the scope that this instructions is recorded.
The above embodiment only have expressed several embodiment of the present invention, and it describes comparatively concrete and detailed, but can not therefore be construed as limiting the scope of the patent.It should be pointed out that for the person of ordinary skill of the art, without departing from the inventive concept of the premise, can also make some distortion and improvement, these all belong to protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
Claims (10)
1. the detection method of azobisformamide content in food, is characterized in that, comprise the steps:
(1) take a certain amount of food, add dimethyl formamide and dissolve, filter, obtain liquid to be measured;
(2) with the content of azobisformamide in high effective liquid chromatography for measuring liquid to be measured, with the phosphate aqueous solution of pH=3 for mobile phase, photodiode array detector is adopted.
2. detection method according to claim 1, it is characterized in that, described a certain amount of food, is less than 2 grams.
3. detection method according to claim 2, is characterized in that, further comprising the steps of before described step (1): to be pulverized by food.
4. detection method according to claim 3, is characterized in that, described food is crushed to its maximum particle diameter and is less than 1mm.
5. detection method according to claim 1, is characterized in that, adopts organic filter membrane to carry out described filter operation in described step (1).
6. detection method according to claim 5, it is characterized in that, the specification of described organic filter membrane is 0.45 μm.
7. detection method according to claim 1, it is characterized in that, the described mobile phase in described step (2) is placed in ultrasonic cleaner ultrasonic through 0.45 μm of organic membrane filtration before use.
8. detection method according to claim 7, it is characterized in that, the described ultrasonic time is 10min ~ 15min.
9. detection method according to claim 8, it is characterized in that, the described ultrasonic time is 10min.
10. according to the arbitrary described detection method of claim 1 to 9, it is characterized in that, C18 chromatographic column selected by described high performance liquid chromatography, and described chromatographic column internal diameter is 250mm*4.6mm, and particle diameter is 5 μm.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107589195A (en) * | 2017-11-02 | 2018-01-16 | 苏州出入境检验检疫局检验检疫综合技术中心 | The detection method of azodicarbonamide in a kind of stickiness Flour product |
| CN107917968A (en) * | 2017-04-10 | 2018-04-17 | 泰州市产品质量监督检验中心 | A kind of method of azodicarbonamide content in high effective liquid chromatography for measuring leather |
| CN109781906A (en) * | 2019-03-18 | 2019-05-21 | 珠海格力电器股份有限公司 | The method of azodicarbonamide in ultra performance liquid chromatography-PDA combination detection sponge product |
| CN115266979A (en) * | 2022-07-27 | 2022-11-01 | 上海市质量监督检验技术研究院 | Method for determining azodicarbonamide in textiles and artificial leather |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102841172A (en) * | 2012-09-27 | 2012-12-26 | 苏州市产品质量监督检验所 | High performance liquid chromatography (HPLC) detection method for azodicarbonamide (ADA) |
-
2014
- 2014-11-21 CN CN201410682713.0A patent/CN104407070B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102841172A (en) * | 2012-09-27 | 2012-12-26 | 苏州市产品质量监督检验所 | High performance liquid chromatography (HPLC) detection method for azodicarbonamide (ADA) |
Non-Patent Citations (4)
| Title |
|---|
| BRIAN G. OSBORNE: "High-performance liquid chromatography of azodicarbonamide", 《JOURNAL OF CHROMATOGRAPHY》 * |
| 周漪波等: "小麦粉中偶氮甲酰胺的高效淮相色谱法快速测定", 《食品研究与开发》 * |
| 弓耀忠等: "高效液相色谱法检测小麦粉中的偶氮甲酰胺方法的研究", 《分析测试》 * |
| 陈波等: "面粉中偶氮甲酰胺含量的高效液相色谱法测定", 《分析测试学报》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107917968A (en) * | 2017-04-10 | 2018-04-17 | 泰州市产品质量监督检验中心 | A kind of method of azodicarbonamide content in high effective liquid chromatography for measuring leather |
| CN107589195A (en) * | 2017-11-02 | 2018-01-16 | 苏州出入境检验检疫局检验检疫综合技术中心 | The detection method of azodicarbonamide in a kind of stickiness Flour product |
| CN109781906A (en) * | 2019-03-18 | 2019-05-21 | 珠海格力电器股份有限公司 | The method of azodicarbonamide in ultra performance liquid chromatography-PDA combination detection sponge product |
| CN115266979A (en) * | 2022-07-27 | 2022-11-01 | 上海市质量监督检验技术研究院 | Method for determining azodicarbonamide in textiles and artificial leather |
| CN115266979B (en) * | 2022-07-27 | 2024-02-23 | 上海市质量监督检验技术研究院 | Method for measuring azodicarbonamide in textile and artificial leather |
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Address after: No.7, Gongye North 4th Road, Songshanhu high tech Industrial Development Zone, Dongguan City, Guangdong Province, 523000 Patentee after: Guangdong Zhongding Testing Technology Co.,Ltd. Address before: 523808 No.7, Gongye North 4th Road, Songshanhu high tech Industrial Development Zone, Dongguan City, Guangdong Province Patentee before: DONGGUAN CONSUMER TESTING TECHNOLOGY Co.,Ltd. |