CN102911513A - Extraction method of gardenia yellow pigment - Google Patents
Extraction method of gardenia yellow pigment Download PDFInfo
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- CN102911513A CN102911513A CN2012102929460A CN201210292946A CN102911513A CN 102911513 A CN102911513 A CN 102911513A CN 2012102929460 A CN2012102929460 A CN 2012102929460A CN 201210292946 A CN201210292946 A CN 201210292946A CN 102911513 A CN102911513 A CN 102911513A
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Abstract
The invention discloses an extraction method of gardenia yellow pigment, comprising the steps of: (A) crushing peeled gardenia to obtain gardenia particles, adding water to the gardenia particles and leaching the gardenia particles to obtain leachate; (2) carrying out solid-liquid separation on the leachate to obtain supernatant, and carrying out microfiltration on the supernatant to obtain first filtrate; (C) adsorbing gardenia yellow pigment in the first filtrate utilizing resin, and eluting the resin to obtain eluate; and (D) carrying out ultrafiltration on the eluate to obtain second filtrate, and finally drying the second filtrate to obtain gardenia yellow pigment. By utilizing the extraction method disclosed by the embodiment of the invention, gardenia yellow pigment with high color number, high purity, high quality and long storage life can be obtained; and the obtained gardenia yellow pigment does not turn into green or grey after long-term storage.
Description
Technical field
The present invention relates to a kind of extracting method of Gardenia Yellow, in particular to a kind of method of from cape jasmine, extracting Gardenia Yellow.
Background technology
Because the synthetic colour extraction cost is low, and stable in properties, strong coloring force, so synthetic colour is widely used.But, along with going deep into of medical science toxicology and biological study work, find that synthetic colour has potential teratogenesis, carcinogenic and other toxic side effect, so synthetic colour is substituted by natural pigment gradually.Gardenia Yellow is a kind of natural pigment that extracts from the fruit of madder wort cape jasmine, has strong, safe, the health protection effect of stability.
Yet also there is following defective in existing Jasmin uranidin prodn. tech: 1, the look valency of resulting Gardenia Yellow not high (below 300); 2, green change and ash change can occur in resulting Gardenia Yellow after storing for some time, a little precipitation occurs, affect quality product, and namely the storage time is short.
Summary of the invention
One object of the present invention is to propose a kind of extracting method that can obtain high look valency, high purity, the high-quality and Gardenia Yellow that storage period is long.
For achieving the above object, a kind of extracting method of Gardenia Yellow is proposed according to the present invention, described extracting method comprises: A) cape jasmine of processing through peeling is pulverized in order to obtain the cape jasmine powder, then in described cape jasmine powder, add entry and described cape jasmine powder is carried out lixiviate, obtain vat liquor; B) described vat liquor is carried out solid-liquid separation and obtains supernatant liquor, then described supernatant liquor is carried out micro-filtration and obtains the first filtrate; C) utilize Gardenia Yellow in described the first filtrate of resin absorption, then described resin is carried out wash-out and obtains elutriant; And D) described elutriant is carried out ultrafiltration and obtains the second filtrate, at last described the second filtrate is carried out drying in order to obtain Gardenia Yellow.
Because cape jasmine has passed through the peeling processing, therefore the content of impurity in the described vat liquor can be reduced significantly, thereby the absorption load of described resin can be alleviated widely, improved the adsorption efficiency of described resin.And, according to the extracting method of the embodiment of the invention by before described resin absorption Gardenia Yellow, described supernatant liquor being carried out micro-filtration, thereby can further alleviate the absorption load of described resin, thereby further improved the adsorption efficiency of described resin.Therefore, by utilizing the extracting method according to the embodiment of the invention, can obtain high look valency, high purity, the Gardenia Yellow that high-quality and storage period is long, green change does not occur after standing storage resulting Gardenia Yellow and ash becomes.Extracting method according to the embodiment of the invention also has the advantages such as simple, with short production cycle, the suitable scale operation of production technique.
In addition, the extracting method according to the embodiment of the invention can have following additional technical characterictic:
According to one embodiment of present invention, in described steps A) in, the particle diameter of described cape jasmine powder is 1-10mm, the mass ratio of described cape jasmine powder and described water is 1:10-15, adds the 1-3% polygalacturonase in the mixture of described cape jasmine powder and described water and described lixiviate is carried out 1h-4h under 25 ℃-40 ℃ condition.
According to one embodiment of present invention, when carrying out described lixiviate, in the mixture of described cape jasmine powder and described water, add microwave.
According to one embodiment of present invention, at described step B) in, utilize the aperture for the first inorganic ceramic film of 50nm-1000nm described supernatant liquor to be carried out described micro-filtration.
According to one embodiment of present invention, at described step B) in, the working pressure of described micro-filtration is 0.15MPa-0.3MPa, the crossflow velocity of described micro-filtration is 2m/s-4m/s.
According to one embodiment of present invention, at described step C) in, described resin is macroporous resin, and utilize ethanolic soln that described resin is carried out wash-out, the volume ratio of wherein said resin and described ethanolic soln is 1:4-6, and the elution flow rate of described ethanolic soln is 1.0ml/min-2.0ml/min.
According to one embodiment of present invention, at described step C) in, utilize first concentration for the ethanolic soln of 25%-35% described resin to be carried out wash-out, the ethanolic soln of recycling 45%-55% carries out wash-out to described resin.
According to one embodiment of present invention, at described step D) in, utilize the aperture for the second inorganic ceramic film of 5nm-20nm described elutriant to be carried out described ultrafiltration.
According to one embodiment of present invention, at described step D) in, the working pressure of described ultrafiltration is 0.15MPa-0.3MPa, the crossflow velocity of described ultrafiltration is 2m/s-4m/s.
According to one embodiment of present invention, at described step D) in, first described the second filtrate is carried out vacuum concentration, carried out spraying drying again.
Additional aspect of the present invention and advantage in the following description part provide, and part will become obviously from the following description, or recognize by practice of the present invention.
Embodiment
The below describes embodiments of the invention in detail.
Extracting method according to the embodiment of the invention is described below.Extracting method according to the embodiment of the invention comprises:
A) cape jasmine of processing through peeling is pulverized in order to obtain the cape jasmine powder, then in described cape jasmine powder, add entry and described cape jasmine powder is carried out lixiviate, obtain vat liquor;
B) described vat liquor is carried out solid-liquid separation and obtains supernatant liquor, then described supernatant liquor is carried out micro-filtration and obtains the first filtrate;
C) utilize Gardenia Yellow in described the first filtrate of resin absorption, then described resin is carried out wash-out and obtains elutriant;
D) described elutriant is carried out ultrafiltration and obtains the second filtrate, at last described the second filtrate is carried out drying in order to obtain Gardenia Yellow.
Because cape jasmine has passed through the peeling processing, therefore the content of impurity in the described vat liquor can be reduced significantly, thereby the absorption load of described resin can be alleviated widely, improved the adsorption efficiency of described resin.And, according to the extracting method of the embodiment of the invention by before described resin absorption Gardenia Yellow, described supernatant liquor being carried out micro-filtration, thereby can further alleviate the absorption load of described resin, thereby further improved the adsorption efficiency of described resin.Therefore, by utilizing the extracting method according to the embodiment of the invention, can obtain high look valency, high purity, the Gardenia Yellow that high-quality and storage period is long, green change does not occur after standing storage resulting Gardenia Yellow and ash becomes.Extracting method according to the embodiment of the invention also has the advantages such as simple, with short production cycle, the suitable scale operation of production technique.
Wherein, cape jasmine refers to the fruit of madder wort cape jasmine (Gardenia jasminoides Ellis).
Can clean with dry in order to obtain dry cape jasmine fresh cape jasmine first.Cape jasmine to drying carries out the peeling processing.Then, can utilize high speed disintegrator that cape jasmine is pulverized, and obtain the cape jasmine powder that particle diameter is 1-10mm.Subsequently described cape jasmine powder is joined in the lixiviate container, and to add quality in the described lixiviate container be 10 times to 15 times the water (described water is preferably purified water, can avoid introducing like this impurity) of the quality of described cape jasmine powder.
Can utilize enzyme hydrolysis method that described cape jasmine powder is carried out lixiviate (enzyme can crack the cell walls of cape jasmine), described lixiviate is carried out under lower temperature in order to avoid destroying the activity of Gardenia Yellow.Preferably, can utilize polygalacturonase or proteolytic enzyme that described cape jasmine powder is carried out lixiviate.Most preferably, can utilize polygalacturonase that described cape jasmine powder is carried out lixiviate.Particularly, can in the mixture of cape jasmine powder and water, add the 1-3%(weight percent) polygalacturonase.That is to say, the weight ratio of cape jasmine powder and polygalacturonase can be 1:0.01-0.03.
Described lixiviate can be carried out under 25 ℃-40 ℃ condition.Preferably, described lixiviate can be carried out under 30 ℃-40 ℃ condition.Further preferably, described lixiviate can be carried out under 35 ℃ condition, can make like this activity of polygalacturonase reach maximum.Described lixiviate can be carried out 1-4 time, and the vat liquor that each lixiviate obtains is merged.Advantageously, described lixiviate can be carried out 2-3 time, so not only can with the Gardenia Yellow in the described cape jasmine powder as much as possible lixiviate go out, and can shorten extraction time, improve extracting efficiency.The time of each lixiviate can be 1h-4h.Preferably, the time of each lixiviate can be 2h-3h, so not only can with the Gardenia Yellow in the described cape jasmine powder as much as possible lixiviate go out, and can shorten extraction time, raising extracting efficiency.
Preferably, when carrying out described lixiviate, can in the mixture of described cape jasmine powder and described water, add microwave, so not only can with the Gardenia Yellow in the described cape jasmine powder as much as possible lixiviate go out, and can shorten extraction time, improve extracting efficiency.Then, can carry out solid-liquid separation in order to remove suspended solid and cape jasmine wet goods in the described vat liquor to described vat liquor.Preferably, can carry out described vat liquor centrifugally, for example utilize dull and stereotyped whizzer to carry out centrifugal to described vat liquor.
Then, the supernatant liquor that described solid-liquid separation is obtained carries out micro-filtration and obtains the first filtrate.Preferably, can utilize the aperture for the first inorganic ceramic film of 50nm-1000nm described supernatant liquor to be carried out described micro-filtration in order to remove high molecular weight protein, polysaccharide and microorganism etc. in the described supernatant liquor.Preferably, the aperture of described the first inorganic ceramic film can be 50nm-800nm.Further preferably, the aperture of described the first inorganic ceramic film can be 50nm-500nm.Most preferably, the aperture of described the first inorganic ceramic film can be 50nm, so not only can all remove high molecular weight protein, polysaccharide and microorganism etc. in the described supernatant liquor, thereby alleviate widely the absorption load of described resin, the adsorption efficiency of the described resin of raising, and can reduce widely described the first inorganic ceramic membrane washing difficulty, described the first inorganic ceramic film is easily regenerated.
Particularly, can utilize topping-up pump that described supernatant liquor is carried out supercharging, then be that the described supernatant liquor of supercharging is through described the first inorganic ceramic film.At described step B) in, the working pressure of described micro-filtration can be 0.15MPa-0.3MPa, the crossflow velocity of described micro-filtration can be 2m/s-4m/s.Preferably, the working pressure of described micro-filtration can be 0.2MPa-0.3MPa, and the crossflow velocity of described micro-filtration can be 2.5m/s-3.5m/s.Further preferably, the working pressure of described micro-filtration can be 0.25MPa, and the crossflow velocity of described micro-filtration can be 3.0m/s.Described supernatant liquor is carried out can obtaining described the first filtrate and the first concentrated solution behind the described micro-filtration.Described the first concentrated solution can return solid-liquid separation step and circulate.
Preferably, can utilize Gardenia Yellow in described the first filtrate of macroporous resin (for example SX-2 macroporous resin) absorption.Can utilize ethanolic soln that described macroporous resin is carried out wash-out and obtains elutriant.Wherein, the volume ratio of described macroporous resin and described ethanolic soln can be 1:4-6, and the flow velocity of described ethanolic soln can be 1.0ml/min-2.0ml/min.Further preferably, the volume ratio of described macroporous resin and described ethanolic soln can be 1:5, and the elution flow rate of described ethanolic soln can be 1.5ml/min.
Can utilize described ethanolic soln that described macroporous resin is carried out gradient elution.Particularly, can utilize first concentration for the ethanolic soln of 25%-35% described macroporous resin to be carried out wash-out in order to the impurity of described macroporous resin adsorption is washed away, the ethanolic soln that then can utilize 45%-55% carries out wash-out to described resin in order to the Gardenia Yellow of described macroporous resin adsorption is washed away.Preferably, can utilize first concentration is that 30% ethanolic soln carries out wash-out to described macroporous resin in order to the impurity of described macroporous resin adsorption is all washed away, and then can utilize 50% ethanolic soln that described resin is carried out wash-out in order to the Gardenia Yellow of described macroporous resin adsorption is all washed away.Wherein, the volume sum of 30% ethanolic soln and 50% ethanolic soln is 5 times of volume of described macroporous resin.
Then, described elutriant is carried out ultrafiltration and obtains the second filtrate.Preferably, can utilize the aperture for the second inorganic ceramic film of 5nm-20nm described elutriant to be carried out described ultrafiltration in order to remove fine impurity in the described elutriant, can reduce widely like this turbidity of Gardenia Yellow.Preferably, the aperture of described the second inorganic ceramic film can be 10nm-15nm.Most preferably, the aperture of described the second inorganic ceramic film can be 15nm, so not only can all remove the fine impurity in the described elutriant, and can reduce widely described the second inorganic ceramic membrane washing difficulty, described the second inorganic ceramic film is easily regenerated.
At described step D) in, the working pressure of described ultrafiltration can be 0.15MPa-0.3MPa, the crossflow velocity of described ultrafiltration can be 2m/s-4m/s.Preferably, the working pressure of described ultrafiltration can be 0.2MPa-0.3MPa, and the crossflow velocity of described ultrafiltration can be 2.5m/s-3.5m/s.Further preferably, the working pressure of described ultrafiltration can be 0.25MPa, and the crossflow velocity of described ultrafiltration can be 3.0m/s.Described elutriant is carried out can obtaining described the second filtrate and the second concentrated solution after the described ultrafiltration.Described the second concentrated solution can return solid-liquid separation step and circulate.
Can directly carry out drying in order to obtain Gardenia Yellow to described the second filtrate.Preferably, can carry out vacuum concentration, carry out drying (for example spraying drying) again described the second filtrate first, can improve widely rate of drying like this.
Preferably, in described the first inorganic ceramic film and described the second inorganic ceramic film each can be with metal oxide as supporter, and applied the isolating active layer of centrifugation at described supporter, can be intermediate layer between described supporter and the described isolating active layer.In other words, each in described the first inorganic ceramic film and described the second inorganic ceramic film can be multilayered structure.Aperture homogeneous and the described isolating active layer of described first inorganic ceramic film of multilayered structure and described second inorganic ceramic film of multilayered structure are thin, compare with organic membrane have long service life, easy cleaning regeneration, high temperature resistant, corrosion-resistant, intensity advantages of higher, compare with metallic membrane and to have the characteristics such as corrosion-resistant.
Embodiment 1
Utilize high speed disintegrator (model: XL-880, Hangzhou match rising sun food machinery company limited) the cape jasmine dry fruit of 50kg being processed through peeling is pulverized, obtaining particle diameter is the cape jasmine powder of 1-10mm, then adds 500kg purified water and 1% polygalacturonase in described cape jasmine powder.Under 25 ℃ condition, carry out the lixiviate of 4h and obtain vat liquor.
Utilize dull and stereotyped whizzer (model: LD-800, Zhangjiagang Xinhua Chemistry Machine Co., Ltd.) described vat liquor is carried out solid-liquid separation and obtains supernatant liquor, described supernatant liquor is that the first inorganic ceramic film of 50nm is to carry out micro-filtration by the aperture after through the topping-up pump supercharging.The working pressure of described micro-filtration is 0.15MPa, and the crossflow velocity of described micro-filtration is 2m/s.
Utilize the Gardenia Yellow in SX-2 macroporous resin adsorption the first filtrate, then utilize first 25% ethanolic soln that the SX-2 macroporous resin is carried out the wash-out removal of impurities, the ethanolic soln of recycling 45% carries out wash-out to the SX-2 macroporous resin.Wherein, the volume sum of 25% ethanolic soln and 45% ethanolic soln is 4 times of volume of described macroporous resin, and the elution flow rate of ethanolic soln is 1.0ml/min.
With elutriant by the aperture be the second inorganic ceramic film of 5nm to carry out ultrafiltration, the working pressure of described ultrafiltration is 0.15MPa, the crossflow velocity of described ultrafiltration is 2m/s.At last the second filtrate is carried out vacuum concentration and spraying drying in order to obtain Gardenia Yellow.In the present embodiment, the look valency of Gardenia Yellow is 450, stores green change and ash change can not occur after 3 months.
Embodiment 2
Utilize high speed disintegrator that the cape jasmine dry fruit that 50kg processes through peeling is pulverized, obtaining particle diameter is the cape jasmine powder of 1-10mm, then adds 750kg purified water and 2% polygalacturonase in described cape jasmine powder.Carry out altogether 2 lixiviates, 1h is carried out in each lixiviate under 40 ℃ condition, and the vat liquor that then each lixiviate is obtained merges.
Utilize dull and stereotyped whizzer that described vat liquor is carried out solid-liquid separation and obtains supernatant liquor, described supernatant liquor is that the first inorganic ceramic film of 1000nm is to carry out micro-filtration by the aperture after through the topping-up pump supercharging.The working pressure of described micro-filtration is 0.3MPa, and the crossflow velocity of described micro-filtration is 4m/s.
Utilize the Gardenia Yellow in SX-3 macroporous resin adsorption the first filtrate, then utilize first 35% ethanolic soln that the SX-3 macroporous resin is carried out the wash-out removal of impurities, the ethanolic soln of recycling 55% carries out wash-out to the SX-3 macroporous resin.Wherein, the volume sum of 35% ethanolic soln and 55% ethanolic soln is 6 times of volume of described macroporous resin, and the elution flow rate of ethanolic soln is 2.0ml/min.
With elutriant by the aperture be the second inorganic ceramic film of 20nm to carry out ultrafiltration, the working pressure of described ultrafiltration is 0.3MPa, the crossflow velocity of described ultrafiltration is 4m/s.At last the second filtrate is carried out vacuum concentration and spraying drying in order to obtain Gardenia Yellow.In the present embodiment, the look valency of Gardenia Yellow is 400, stores green change and ash change can not occur after 2.5 months.
Embodiment 3
Utilize high speed disintegrator that the cape jasmine dry fruit that 50kg processes through peeling is pulverized, obtaining particle diameter is the cape jasmine powder of 1-20mm, then adds 600kg purified water and 3% polygalacturonase in described cape jasmine powder.Carry out altogether 4 lixiviates, 2h is carried out in each lixiviate under 30 ℃ condition, and the vat liquor that then each lixiviate is obtained merges.
Utilize dull and stereotyped whizzer that described vat liquor is carried out solid-liquid separation and obtains supernatant liquor, described supernatant liquor is that the first inorganic ceramic film of 100nm is to carry out micro-filtration by the aperture after through the topping-up pump supercharging.The working pressure of described micro-filtration is 0.2MPa, and the crossflow velocity of described micro-filtration is 2.5m/s.
Utilize the Gardenia Yellow in HPD400 macroporous resin adsorption the first filtrate, then utilize first 30% ethanolic soln that the HPD400 macroporous resin is carried out the wash-out removal of impurities, the ethanolic soln of recycling 50% carries out wash-out to the HPD400 macroporous resin.Wherein, the volume sum of 30% ethanolic soln and 50% ethanolic soln is 5 times of volume of described macroporous resin, and the elution flow rate of ethanolic soln is 1.5ml/min.
With elutriant by the aperture be the second inorganic ceramic film of 10nm to carry out ultrafiltration, the working pressure of described ultrafiltration is 0.2MPa, the crossflow velocity of described ultrafiltration is 2.5m/s.At last the second filtrate is carried out vacuum concentration and spraying drying in order to obtain Gardenia Yellow.In the present embodiment, the look valency of Gardenia Yellow is 430, stores green change and ash change can not occur after 3 months.
Embodiment 4
Utilize high speed disintegrator that the cape jasmine dry fruit that 50kg processes through peeling is pulverized, obtaining particle diameter is the cape jasmine powder of 1-15mm, then adds 600kg purified water and 1% polygalacturonase in described cape jasmine powder.Carry out altogether 3 lixiviates, 3h is carried out in each lixiviate under 35 ℃ condition, and the vat liquor that then each lixiviate is obtained merges.
Utilize dull and stereotyped whizzer that described vat liquor is carried out solid-liquid separation and obtains supernatant liquor, described supernatant liquor is that the first inorganic ceramic film of 500nm is to carry out micro-filtration by the aperture after through the topping-up pump supercharging.The working pressure of described micro-filtration is 0.25MPa, and the crossflow velocity of described micro-filtration is 3.5m/s.
Utilize the Gardenia Yellow in SX-2 macroporous resin adsorption the first filtrate, then utilize first 30% ethanolic soln that the SX-2 macroporous resin is carried out the wash-out removal of impurities, the ethanolic soln of recycling 50% carries out wash-out to the SX-2 macroporous resin.Wherein, the volume sum of 30% ethanolic soln and 50% ethanolic soln is 5 times of volume of described macroporous resin, and the elution flow rate of ethanolic soln is 1.5ml/min.
With elutriant by the aperture be the second inorganic ceramic film of 15nm to carry out ultrafiltration, the working pressure of described ultrafiltration is 0.25MPa, the crossflow velocity of described ultrafiltration is 3.5m/s.At last the second filtrate is carried out vacuum concentration and spraying drying in order to obtain Gardenia Yellow.In the present embodiment, the look valency of Gardenia Yellow is 450, stores green change and ash change can not occur after 3 months.
Embodiment 5
Utilize high speed disintegrator that the cape jasmine dry fruit that 50kg processes through peeling is pulverized, obtaining particle diameter is the cape jasmine powder of 1-10mm, then adds 600kg purified water and 3% polygalacturonase in described cape jasmine powder.Carry out altogether 3 lixiviates, 3h is carried out in each lixiviate under 35 ℃ condition, and the vat liquor that then each lixiviate is obtained merges.
Utilize dull and stereotyped whizzer that described vat liquor is carried out solid-liquid separation and obtains supernatant liquor, described supernatant liquor is that the first inorganic ceramic film of 200nm is to carry out micro-filtration by the aperture after through the topping-up pump supercharging.The working pressure of described micro-filtration is 0.25MPa, and the crossflow velocity of described micro-filtration is 3.0m/s.
Utilize the Gardenia Yellow in SX-3 macroporous resin adsorption the first filtrate, then utilize first 30% ethanolic soln that the SX-3 macroporous resin is carried out the wash-out removal of impurities, the ethanolic soln of recycling 50% carries out wash-out to the SX-3 macroporous resin.Wherein, the volume sum of 30% ethanolic soln and 50% ethanolic soln is 5 times of volume of described macroporous resin, and the elution flow rate of ethanolic soln is 1.5ml/min.
With elutriant by the aperture be the second inorganic ceramic film of 15nm to carry out ultrafiltration, the working pressure of described ultrafiltration is 0.25MPa, the crossflow velocity of described ultrafiltration is 3.0m/s.At last the second filtrate is carried out vacuum concentration and spraying drying in order to obtain Gardenia Yellow.In the present embodiment, the look valency of Gardenia Yellow is 480, stores green change and ash change can not occur after 4 months.
The look valency refers to the absorbancy of the extract of unit mass raw material, can measure according to potassium dichromate solution colorimetry among the GB5525.Storage time is by after storing for some time, estimates its color and whether turns green change ash or have precipitation to produce.
In the description of this specification sheets, the description of reference term " embodiment ", " some embodiment ", " example ", " concrete example " or " some examples " etc. means to be contained at least one embodiment of the present invention or the example in conjunction with specific features, structure, material or the characteristics of this embodiment or example description.In this manual, the schematic statement of above-mentioned term not necessarily referred to identical embodiment or example.And the specific features of description, structure, material or characteristics can be with suitable mode combinations in any one or more embodiment or example.
Although illustrated and described embodiments of the invention, those having ordinary skill in the art will appreciate that: in the situation that do not break away from principle of the present invention and aim can be carried out multiple variation, modification, replacement and modification to these embodiment, scope of the present invention is limited by claim and equivalent thereof.
Claims (10)
1. the extracting method of a Gardenia Yellow is characterized in that, described extracting method comprises:
A) cape jasmine of processing through peeling is pulverized in order to obtain the cape jasmine powder, then in described cape jasmine powder, add entry and described cape jasmine powder is carried out lixiviate, obtain vat liquor;
B) described vat liquor is carried out solid-liquid separation and obtains supernatant liquor, then described supernatant liquor is carried out micro-filtration and obtains the first filtrate;
C) utilize Gardenia Yellow in described the first filtrate of resin absorption, then described resin is carried out wash-out and obtains elutriant;
D) described elutriant is carried out ultrafiltration and obtains the second filtrate, at last described the second filtrate is carried out drying in order to obtain Gardenia Yellow.
2. extracting method according to claim 1, it is characterized in that, in described steps A) in, the particle diameter of described cape jasmine powder is 1-10mm, the mass ratio of described cape jasmine powder and described water is 1:10-15, adds the 1-3% polygalacturonase in the mixture of described cape jasmine powder and described water and described lixiviate is carried out 1h-4h under 25 ℃-40 ℃ condition.
3. extracting method according to claim 1 and 2 is characterized in that, when carrying out described lixiviate, adds microwave in the mixture of described cape jasmine powder and described water.
4. extracting method according to claim 1 is characterized in that, at described step B) in, utilize the aperture for the first inorganic ceramic film of 50nm-1000nm described supernatant liquor to be carried out described micro-filtration.
5. extracting method according to claim 4 is characterized in that, at described step B) in, the working pressure of described micro-filtration is 0.15MPa-0.3MPa, the crossflow velocity of described micro-filtration is 2m/s-4m/s.
6. extracting method according to claim 1, it is characterized in that, at described step C) in, described resin is macroporous resin, and utilize ethanolic soln that described resin is carried out wash-out, the volume ratio of wherein said resin and described ethanolic soln is 1:4-6, and the elution flow rate of described ethanolic soln is 1.0ml/min-2.0ml/min.
7. extracting method according to claim 6 is characterized in that, at described step C) in, utilize first concentration for the ethanolic soln of 25%-35% described resin to be carried out wash-out, the ethanolic soln of recycling 45%-55% carries out wash-out to described resin.
8. extracting method according to claim 1 is characterized in that, at described step D) in, utilize the aperture for the second inorganic ceramic film of 5nm-20nm described elutriant to be carried out described ultrafiltration.
9. extracting method according to claim 8 is characterized in that, at described step D) in, the working pressure of described ultrafiltration is 0.15MPa-0.3MPa, the crossflow velocity of described ultrafiltration is 2m/s-4m/s.
10. extracting method according to claim 1 is characterized in that, at described step D) in, first described the second filtrate is carried out vacuum concentration, carried out spraying drying again.
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| CN103735633A (en) * | 2013-12-30 | 2014-04-23 | 南京博士牛生物科技有限公司 | Preparation method of instant gardenia powder |
| CN103740130A (en) * | 2014-01-11 | 2014-04-23 | 云南瑞宝生物科技有限公司 | Preparation method of gardenia yellow pigment |
| CN108774407A (en) * | 2018-05-30 | 2018-11-09 | 山东省农业科学院农产品研究所 | A kind of extracting method of gardenia yellow pigment with high color value |
| CN112724703A (en) * | 2021-03-05 | 2021-04-30 | 中国林业科学研究院林产化学工业研究所 | Method for extracting gardenia yellow pigment |
| CN112812585A (en) * | 2020-12-26 | 2021-05-18 | 无锡济煜山禾药业股份有限公司 | Method for recovering gardenin from distillation waste liquid of Xingnaojing injection |
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| CN106084878A (en) * | 2016-07-15 | 2016-11-09 | 广西顺帆投资有限公司 | A kind of method extracting dyestuff based on Semen Momordicae fruit |
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| CN112812585A (en) * | 2020-12-26 | 2021-05-18 | 无锡济煜山禾药业股份有限公司 | Method for recovering gardenin from distillation waste liquid of Xingnaojing injection |
| CN112724703A (en) * | 2021-03-05 | 2021-04-30 | 中国林业科学研究院林产化学工业研究所 | Method for extracting gardenia yellow pigment |
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