CN102908338A - Preparation method of resveratrol microcapsule - Google Patents
Preparation method of resveratrol microcapsule Download PDFInfo
- Publication number
- CN102908338A CN102908338A CN2012104731647A CN201210473164A CN102908338A CN 102908338 A CN102908338 A CN 102908338A CN 2012104731647 A CN2012104731647 A CN 2012104731647A CN 201210473164 A CN201210473164 A CN 201210473164A CN 102908338 A CN102908338 A CN 102908338A
- Authority
- CN
- China
- Prior art keywords
- resveratrol
- mass ratio
- colloid mill
- chitosan
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicinal Preparation (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
The invention discloses a preparation method of a resveratrol microcapsule, and the method comprises the following steps of: (1) dissolving resveratrol into a solvent, adding an emulsifying agent, and evenly mixing to obtain the core material solution by means of colloid mill; (2) adding water into chitosan, stirring, and swelling and dispersing the chitosan to prepare a wall material solution; and (3) stirring the core material solution and adding the core material solution into the wall material solution to obtain a mixed solution, homogenizing and emulsifying into white lactescence by means of colloid mill, adding a curing agent, continuously homogenizing by means of a colloid mill, and carrying out spray drying to obtain the resveratrol microcapsule with good dispersity. The resveratrol microcapsule prepared by the method provided by the invention is good in solubility and high in stability. The method provided by the invention is simple to operate, and easy for industrial production.
Description
Technical field
The present invention relates to the preparation method of Resveratrol microcapsule.
Background technology
Resveratrol is a kind of polyphenol compound that extensively is present in the higher plant; it is a kind of natural antioxidant; has blood viscosity lowering; anti-platelet clotting and vasodilation; keep unblocked blood flowing; have slow down aging, regulate blood fat, the chronic inflammatory disease that is caused by pathogenic microorganism infection can be prevented and treat to the functions such as protection cardiovascular and cerebrovascular vessel, anti-hepatitis.Contain many complement activity factors in the resveratrol, to the human body beneficial.The researcher points out, uses resveratrol can effectively treat cardiovascular disease and cancer.
The microencapsulation technology is a kind of new technique that develops rapidly over nearly 20 years.It utilizes natural or synthetic macromolecular material with molecule solid, liquid, gas, is wrapped in diameter and is in the microencapsulation that a kind of in 1 ~ 500 μ m scope have semi permeability or sealing cyst membrane.Material by embedding is referred to as core, and encapsulated material is called the wall material.This technology has been widely used in the industries such as medicine, food, chemical industry at present, and it can improve the stability of enveloped confrontation light, heat, oxygen well, control rate of release, the caking that keeps away from moisture, the shelf life of prolongation commodity.
The poorly water-soluble of resveratrol.
Summary of the invention
The method that the purpose of this invention is to provide a kind of preparation of Resveratrol microcapsule by used Novel curing agent in preparation process, can improve the dissolubility of product.
Technical scheme of the present invention is summarized as follows:
A kind of method of preparation of Resveratrol microcapsule comprises the steps:
(1) volumetric concentration that resveratrol is dissolved in as solvent is in the ethanol water of 20%-90%, add emulsifying agent, at the 20-30 ℃ of lower colloid mill 20-40min that passes through, mixing is made core solution, and the mass ratio of described resveratrol, solvent and emulsifying agent is 1:0.5-10:0.1-10;
(2) add entry in chitosan, 30-50 ℃ of lower the stirring 2-3 hour, make chitosan swelling, dispersion, make wall material solution, the mass ratio of described chitosan and water is 1:1-20;
(3) according to mass ratio be the ratio of 1:1-30, under 30-50 ℃, core solution is joined in the wall material solution while stirring, obtain mixed liquor, the colloid mill homogenized milk changes into white emulsion, add firming agent, continue to obtain the Resveratrol microcapsule of good dispersion after the spray drying with colloid mill homogenizing 30-60min, described firming agent is that mass ratio is the solution of vanillin, sodium alginate and the water composition of 1-20:1-20:1-100.
Described step (1) is preferably: it is in 75% the ethanol water that resveratrol is dissolved in volumetric concentration as solvent, add emulsifying agent, at 25 ℃ of lower colloid mill 30min that pass through, mixing is made core solution, and the mass ratio of described resveratrol, solvent and emulsifying agent is 1:2:5.
Described emulsifying agent is preferably: one or both in starch sugar fatty acid ester, sodium lauroyl lactylate and the starch alkenyl succinate sugar ester.
Described step (2) is preferably: add entry in chitosan, 40 ℃ of lower stirrings 1.5 hours, make chitosan swelling, dispersion, make wall material solution, the mass ratio of described chitosan and water is 1:10.
Described step (3) is preferably: be the ratio of 1:20 according to mass ratio, under 40 ℃, core solution is joined in the wall material solution, obtain mixed liquor, the colloid mill homogenized milk changes into white emulsion, add firming agent, continue to obtain the Resveratrol microcapsule of good dispersion after the spray drying with colloid mill homogenizing 45min, described firming agent is that mass ratio is the solution of vanillin, sodium alginate and the water composition of 10:10:50.
Advantage of the present invention is:
1. use the Resveratrol microcapsule of method preparation of the present invention, solved the defective of the poorly water-soluble of resveratrol, enlarged the scope of application of resveratrol.
2. the present invention has used Novel curing agent, makes the product solubility of preparation better, and stability is higher.
3. the present invention is simple to operate, is easy to suitability for industrialized production, for the development of resveratrol provides a feasible technology path.
The specific embodiment
The present invention is further illustrated below in conjunction with specific embodiment.
Embodiment 1
A kind of method of preparation of Resveratrol microcapsule comprises the steps:
(1) resveratrol being dissolved in volumetric concentration as solvent is in 75% the ethanol water, add emulsifying agent starch sugar fatty acid ester, at 25 ℃ of lower colloid mill 30min that pass through, mixing is made core solution, and the mass ratio of described resveratrol, solvent and emulsifying agent is 1:2:5;
(2) add entry in chitosan, 40 ℃ of lower stirrings 1.5 hours, make chitosan swelling, dispersion, make wall material solution, the mass ratio of described chitosan and water is 1:10;
(3) according to mass ratio be the ratio of 1:20, under 40 ℃, core solution is joined in the wall material solution, obtain mixed liquor, the colloid mill homogenized milk changes into white emulsion, add firming agent, continue to obtain the Resveratrol microcapsule of good dispersion after the spray drying with colloid mill homogenizing 45min, described firming agent is that mass ratio is the solution of vanillin, sodium alginate and the water composition of 10:10:50.
Its dissolubility of Resveratrol microcapsule of the present embodiment preparation is 95.6%.
Embodiment 2
A kind of method of preparation of Resveratrol microcapsule comprises the steps:
(1) resveratrol being dissolved in volumetric concentration as solvent is in 20% the ethanol water, add the emulsifying agent sodium lauroyl lactylate, pass through colloid mill 40min at 20 ℃, mixing is made core solution, and the mass ratio of described resveratrol, solvent and emulsifying agent is 1:0.5:0.1;
(2) add entry in chitosan, 40 ℃ of lower stirrings 2 hours, make chitosan swelling, dispersion, make wall material solution, the mass ratio of described chitosan and water is 1:1;
(3) according to mass ratio be the ratio of 1:10, under 30 ℃, core solution is joined in the wall material solution while stirring, obtain mixed liquor, the colloid mill homogenized milk changes into white emulsion, add firming agent, continue to obtain the Resveratrol microcapsule of good dispersion after the spray drying with colloid mill homogenizing 40min, described firming agent is that mass ratio is the solution of vanillin, sodium alginate and the water composition of 1:20:1;
Its dissolubility of Resveratrol microcapsule of the present embodiment preparation is 92.3%.
Embodiment 3
A kind of method of preparation of Resveratrol microcapsule comprises the steps:
(1) resveratrol being dissolved in volumetric concentration as solvent is in 50% the ethanol water, add mass ratio and be the starch alkenyl succinate sugar ester of 1:1 and starch sugar fatty acid ester as emulsifying agent, lower to colloid mill 20min at 30 ℃, mixing is made core solution, and the mass ratio of described resveratrol, solvent and emulsifying agent is 1:5:5;
(2) add entry in chitosan, 30 ℃ of lower stirrings 3 hours, make chitosan swelling, dispersion, make wall material solution, the mass ratio of described chitosan and water is 1:20;
(3) according to mass ratio be the ratio of 1:30, under 50 ℃, core solution is joined in the wall material solution while stirring, obtain mixed liquor, the colloid mill homogenized milk changes into white emulsion, add firming agent, continue to obtain the Resveratrol microcapsule of good dispersion after the spray drying with colloid mill homogenizing 30min, described firming agent is that mass ratio is the solution of vanillin, sodium alginate and the water composition of 1:1:10.
Its dissolubility of Resveratrol microcapsule of the present embodiment preparation is 96.7%.
Embodiment 4
A kind of method of preparation of Resveratrol microcapsule comprises the steps:
(1) resveratrol being dissolved in volumetric concentration as solvent is in 90% the ethanol water, add emulsifying agent starch sugar fatty acid ester, at 30 ℃ of lower colloid mill 30min that pass through, mixing is made core solution, and the mass ratio of described resveratrol, solvent and emulsifying agent is 1:10:10;
(2) add entry in chitosan, 50 ℃ of lower stirrings 3 hours, make chitosan swelling, dispersion, make wall material solution, the mass ratio of described chitosan and water is 1:15;
(3) according to mass ratio be the ratio of 1:1, under 40 ℃, core solution is joined in the wall material solution while stirring, obtain mixed liquor, the colloid mill homogenized milk changes into white emulsion, add firming agent, continue to obtain the Resveratrol microcapsule of good dispersion after the spray drying with colloid mill homogenizing 60min, described firming agent is that mass ratio is the solution of vanillin, sodium alginate and the water composition of 20:1:100.
Its dissolubility of Resveratrol microcapsule of the present embodiment preparation is 94.5%.
The dissolubility of encapsulated resveratrol is not 20%
Solubility experiment:
Take by weighing the 1.0g sample and in beaker, use 25 ~ 30 ℃ of water of 7.6ml, with sample dissolution, and move in the 10ml centrifuge tube, place the centrifugal 10min of centrifuge 5000r/min, suck supernatant with liquid-transfering gun, and clean tube wall with tampon.Add again 7.6ml25 ~ 30 ℃ water, fluctuate, precipitation is suspended, put again the centrifugal 10min of 5000r/min in the centrifuge, suck supernatant, and clean tube wall with tampon, with a small amount of water precipitation is washed on known weight, the tared dish that is of moderate size, move into again in 105 ℃ of baking ovens and be dried to constant weight.
Computing formula
Dissolubility (%)=[1--(W
2-W
1)/((1-B%) * W)] * 100%
W-example weight (g); W
1-tared dish heavy (g);
W
2-tared dish weight+insoluble matter heavy (g); B%-sample moisture content.
Claims (5)
1. the method for the preparation of a Resveratrol microcapsule is characterized in that comprising the steps:
(1) volumetric concentration that resveratrol is dissolved in as solvent is in the ethanol water of 20%-90%, add emulsifying agent, at the 20-30 ℃ of lower colloid mill 20-40min that passes through, mixing is made core solution, and the mass ratio of described resveratrol, solvent and emulsifying agent is 1:0.5-10:0.1-10;
(2) add entry in chitosan, 30-50 ℃ of lower the stirring 2-3 hour, make chitosan swelling, dispersion, make wall material solution, the mass ratio of described chitosan and water is 1:1-20;
(3) according to mass ratio be the ratio of 1:1-30, under 30-50 ℃, core solution is joined in the wall material solution while stirring, obtain mixed liquor, the colloid mill homogenized milk changes into white emulsion, add firming agent, continue to obtain the Resveratrol microcapsule of good dispersion after the spray drying with colloid mill homogenizing 30-60min, described firming agent is that mass ratio is the solution of vanillin, sodium alginate and the water composition of 1-20:1-20:1-100.
2. method according to claim 1, it is characterized in that described step (1) is in 75% the ethanol water for resveratrol being dissolved in volumetric concentration as solvent, add emulsifying agent, lower to colloid mill 30min at 25 ℃, mixing is made core solution, and the mass ratio of described resveratrol, solvent and emulsifying agent is 1:2:5.
3. method according to claim 1 and 2 is characterized in that described emulsifying agent is one or both in starch sugar fatty acid ester, sodium lauroyl lactylate and the starch alkenyl succinate sugar ester.
4. method according to claim 1 is characterized in that described step (2) for to add entry in chitosan, 40 ℃ of lower stirrings 1.5 hours, makes chitosan swelling, dispersion, makes wall material solution, and the mass ratio of described chitosan and water is 1:10.
5. method according to claim 1, it is characterized in that described step (3) is for being the ratio of 1:20 according to mass ratio, under 40 ℃, core solution is joined in the wall material solution, obtain mixed liquor, the colloid mill homogenized milk changes into white emulsion, adds firming agent, continues with colloid mill homogenizing 45min, obtain the Resveratrol microcapsule of good dispersion after the spray drying, described firming agent is that mass ratio is the solution of vanillin, sodium alginate and the water composition of 10:10:50.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210473164.7A CN102908338B (en) | 2012-11-20 | 2012-11-20 | Preparation method of resveratrol microcapsule |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210473164.7A CN102908338B (en) | 2012-11-20 | 2012-11-20 | Preparation method of resveratrol microcapsule |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102908338A true CN102908338A (en) | 2013-02-06 |
| CN102908338B CN102908338B (en) | 2014-05-14 |
Family
ID=47607079
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210473164.7A Active CN102908338B (en) | 2012-11-20 | 2012-11-20 | Preparation method of resveratrol microcapsule |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102908338B (en) |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103251053A (en) * | 2013-05-23 | 2013-08-21 | 晨光生物科技集团天津有限公司 | Weight-reducing and beautifying composite and preparation method |
| CN103636794A (en) * | 2013-12-06 | 2014-03-19 | 内蒙古伊利实业集团股份有限公司 | Resveratrol microcapsule suitable for being added to dairy products and preparation method and application thereof |
| CN104223260A (en) * | 2014-09-05 | 2014-12-24 | 晨光生物科技集团天津有限公司 | Solid health beverage containing resveratrol microcapsule and fructo-oligosaccharide |
| CN104644475A (en) * | 2015-03-05 | 2015-05-27 | 临沂大学 | Whitening cream containing resveratrol, apple polyphenol and plant polysaccharide |
| CN104689771A (en) * | 2015-03-05 | 2015-06-10 | 华南农业大学 | Preparation method of oxyresveratrol microcapsules and products thereof |
| CN105385502A (en) * | 2015-12-10 | 2016-03-09 | 上海应用技术学院 | Preparation method of tea tree essential oil nano particle |
| CN107519025A (en) * | 2017-08-23 | 2017-12-29 | 中山大学惠州研究院 | A kind of hydrophobic sun-proof microcapsules and preparation method thereof |
| CN108112954A (en) * | 2017-12-14 | 2018-06-05 | 成都新柯力化工科技有限公司 | A kind of microcapsule food sweetener and preparation method thereof |
| CN110101871A (en) * | 2019-05-30 | 2019-08-09 | 广东工业大学 | A kind of preparation method embedding resveratrol |
| CN114796259A (en) * | 2022-02-17 | 2022-07-29 | 泉州雷恩生化有限公司 | Medicine for inhibiting pulmonary nodule and inflammation and its preparing method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20040084364A (en) * | 2003-03-28 | 2004-10-06 | 주식회사 태평양 | Method of stabilization of active ingredients using polyol/polymer microcapsules and cosmetic composition containing the microcapsules |
| WO2009039195A1 (en) * | 2007-09-20 | 2009-03-26 | Resveratrol Partners, Llc | Resveratrol-containing compositions for modulating gene product concentration or activity |
| CN201657749U (en) * | 2009-12-16 | 2010-12-01 | 烟台开发区绿源生物工程有限公司 | Resveratrol microcapsule |
| CN101927148A (en) * | 2010-07-28 | 2010-12-29 | 河北农业大学 | Method for improving stability and water solubility of resveratrol and application thereof |
| CN102274280A (en) * | 2011-08-10 | 2011-12-14 | 华南农业大学 | Patchouli oil microcapsules and preparation method and application thereof |
-
2012
- 2012-11-20 CN CN201210473164.7A patent/CN102908338B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20040084364A (en) * | 2003-03-28 | 2004-10-06 | 주식회사 태평양 | Method of stabilization of active ingredients using polyol/polymer microcapsules and cosmetic composition containing the microcapsules |
| WO2009039195A1 (en) * | 2007-09-20 | 2009-03-26 | Resveratrol Partners, Llc | Resveratrol-containing compositions for modulating gene product concentration or activity |
| CN201657749U (en) * | 2009-12-16 | 2010-12-01 | 烟台开发区绿源生物工程有限公司 | Resveratrol microcapsule |
| CN101927148A (en) * | 2010-07-28 | 2010-12-29 | 河北农业大学 | Method for improving stability and water solubility of resveratrol and application thereof |
| CN102274280A (en) * | 2011-08-10 | 2011-12-14 | 华南农业大学 | Patchouli oil microcapsules and preparation method and application thereof |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103251053A (en) * | 2013-05-23 | 2013-08-21 | 晨光生物科技集团天津有限公司 | Weight-reducing and beautifying composite and preparation method |
| CN103251053B (en) * | 2013-05-23 | 2014-10-08 | 晨光生物科技集团天津有限公司 | Weight-reducing and beautifying composite and preparation method |
| CN103636794A (en) * | 2013-12-06 | 2014-03-19 | 内蒙古伊利实业集团股份有限公司 | Resveratrol microcapsule suitable for being added to dairy products and preparation method and application thereof |
| CN104223260A (en) * | 2014-09-05 | 2014-12-24 | 晨光生物科技集团天津有限公司 | Solid health beverage containing resveratrol microcapsule and fructo-oligosaccharide |
| CN104644475A (en) * | 2015-03-05 | 2015-05-27 | 临沂大学 | Whitening cream containing resveratrol, apple polyphenol and plant polysaccharide |
| CN104689771A (en) * | 2015-03-05 | 2015-06-10 | 华南农业大学 | Preparation method of oxyresveratrol microcapsules and products thereof |
| CN105385502A (en) * | 2015-12-10 | 2016-03-09 | 上海应用技术学院 | Preparation method of tea tree essential oil nano particle |
| CN105385502B (en) * | 2015-12-10 | 2019-12-03 | 上海应用技术学院 | A kind of preparation method of tea tree ethereal oil nanoparticle |
| CN107519025A (en) * | 2017-08-23 | 2017-12-29 | 中山大学惠州研究院 | A kind of hydrophobic sun-proof microcapsules and preparation method thereof |
| CN108112954A (en) * | 2017-12-14 | 2018-06-05 | 成都新柯力化工科技有限公司 | A kind of microcapsule food sweetener and preparation method thereof |
| CN110101871A (en) * | 2019-05-30 | 2019-08-09 | 广东工业大学 | A kind of preparation method embedding resveratrol |
| CN110101871B (en) * | 2019-05-30 | 2022-03-25 | 广东工业大学 | Preparation method of embedded resveratrol |
| CN114796259A (en) * | 2022-02-17 | 2022-07-29 | 泉州雷恩生化有限公司 | Medicine for inhibiting pulmonary nodule and inflammation and its preparing method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102908338B (en) | 2014-05-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102908338B (en) | Preparation method of resveratrol microcapsule | |
| CN104382024B (en) | A kind of porous starch embedding lycopene is prepared the method for microcapsules | |
| CN105054070B (en) | A kind of Sophora viciifolia color glycosides crude extract and its microcapsules | |
| Liu et al. | Isolation, purification, identification, and stability of anthocyanins from Lycium ruthenicum Murr | |
| CN102511570B (en) | Olive oil microcapsule and preparation method thereof | |
| Pan et al. | Microencapsulation of blueberry anthocyanins by spray drying with soy protein isolates/high methyl pectin combination: Physicochemical properties, release behavior in vitro and storage stability | |
| CN105832569A (en) | Water-dispersible transparent astaxanthin emulsion and preparation method | |
| CN113647624B (en) | Preparation method of curcumin microcapsule | |
| CN102653704A (en) | Preparation method of small peppermint essence soft capsule taking calcium alginate as wall material | |
| CN110037031B (en) | Preparation method of pH/temperature double-sensitive spinosad controlled-release granules | |
| CN107569470B (en) | Preparation method of sulforaphane microcapsule | |
| CN103830744A (en) | Sustained release gallogen-cyclodextrin compound and preparation method thereof | |
| Liu et al. | A novel approach for the simultaneous extraction of dihydroquercetin and arabinogalactan from Larix gmelinii by homogenate-ultrasound-synergistic technique using the ionic liquid | |
| CN103319616A (en) | Ethoxyl octenyl succinate tara gum and preparation method thereof | |
| CN105348165A (en) | Method for extracting astaxanthin yeast, yeast extract and glucan from yeast | |
| CN105622778A (en) | Preparation method of water-soluble chitosan | |
| CN109721740A (en) | A method of continuously preparing the chitin/chitosan solution of different deacetylations | |
| CN106192513A (en) | A kind of method preparing high-purity nano cellulose from fibre plant | |
| CN105693880A (en) | Protein removal method for hericium erinaceus polysaccharide extracting solution | |
| CN102775510A (en) | Preparation technique of low-temperature instant carrageenan | |
| CN105661549A (en) | Preparation method and application of with vitamin B1 and vitamin B6 supported by ferulic acid/chitosan | |
| Pan et al. | A novel soluble powder containing high Dendrobium huoshanense polysaccharide and its in vitro hypoglycemic activities evaluation | |
| CN109486213A (en) | A kind of antibacterial film and preparation method thereof | |
| CN103059223A (en) | Method for synthesizing super absorbent resin through graft copolymerization of alpha-potato starch | |
| CN103230344A (en) | A complex coacervation - spray drying method for preparing idebenone microcapsules |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |