CN102901785A - Temperature uniformizer integrating multiple chromatographic columns with different lengths and diameters - Google Patents
Temperature uniformizer integrating multiple chromatographic columns with different lengths and diameters Download PDFInfo
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- CN102901785A CN102901785A CN201210403661XA CN201210403661A CN102901785A CN 102901785 A CN102901785 A CN 102901785A CN 201210403661X A CN201210403661X A CN 201210403661XA CN 201210403661 A CN201210403661 A CN 201210403661A CN 102901785 A CN102901785 A CN 102901785A
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Abstract
The invention discloses a temperature uniformizer integrating multiple chromatographic columns with different lengths and diameters. An upper metal heat conducting block and a lower metal heat conducting block are linked with each other through a hinge and respectively provided with multiple upper semicircular chromatographic column grooves and multiple lower semicircular chromatographic column grooves, wherein one or more groups of metal inner casings are respectively placed in each upper semicircular chromatographic column groove and each lower semicircular chromatographic column groove, one or more groups of metal outer casings are sheathed outside each metal inner casing, the inner diameter of the metal inner casing is the same as the outer diameter of a body of the chromatographic column placed in the metal inner casing, the outer diameter of the metal outer casing is greater than or equal to that of a head of the chromatographic column, and the length of the upper semicircular chromatographic column groove and the lower semicircular chromatographic column groove is greater than that of the chromatographic column. The temperature uniformizer is reasonable in structure, and can be used for improving the heat conducting efficiency of a thermotank of a high performance liquid chromatograph (HPLC), shortening the heat balance time of the thermotank, and maintaining the consistency, stability and uniformity of the working temperature of the chromatographic columns.
Description
Technical field
The present invention relates to the temperature homogenizer that a kind of different length that uses and post many root chromatogram columns directly are integrated in High Performance Liquid Chromatography post constant temperature oven.
Background technology
The multidimensional high performance liquid chromatography is to utilize the chromatographic column of two or many different in kinds that the component in the complex sample is separated.In the detection of multidimensional high performance liquid chromatography, sample first carries out pre-separation in the first dimension chromatographic column, then will change through all or part of compositional selecting ground of pre-separation further separation in other one or more dissimilar chromatographic column over to.The multidimensional high-efficient liquid phase chromatogram technology has sample is carried out pre-service, and the function of separation and concentration can improve the separating power of high performance liquid chromatograph, can get rid of interfering material from the various ingredients of complexity, analyzes for required component selectively.
The chromatographic column that high performance liquid chromatograph uses has the different length specifications such as 100mm, 150mm, 200mm, 250mm and 300mm usually, also has the different post footpath specification such as 4mm, 5mm, 10mm, 15mm, 20mm and 30mm in the chromatographic column of same length.In the analytical test of multidimensional high performance liquid chromatography, people also can face the situation that need to use simultaneously many different lengths and different posts footpath chromatographic column.The working temperature of many different lengths and different posts footpath chromatographic column correctly is set, keep the post consistent, that stablize and evenly can improve chromatographic column of many different lengths and different posts footpath chromatographic column working temperature to imitate, improve and respectively measure the degree of separation of material chromatographic peak and the symmetry of peak shape, shorten the retention time of measured matter in chromatographic column, reducing the chromatographic column post presses, reduce the wearing and tearing of high performance liquid chromatograph pump, improve sample analysis result's accuracy and repeatability.
When using many different lengths to carry out the analytical test of multidimensional high performance liquid chromatography with the chromatographic column in different posts footpath, people usually set with the chromatographic column constant temperature oven of high performance liquid chromatograph and stablize the working temperature of these different lengths with different posts footpath chromatographic column.Yet its principle of heating of chromatographic column constant temperature oven of high performance liquid chromatograph is to rely on the bottom electric-heating-wire-heating, and air heat conduction is slow.The chromatographic column constant temperature oven of fan-free, hot-air can't circulative convections, and bottom temp is higher when causing heating, and chromatographic column constant temperature oven head temperature is then lower, causes heating of chromatographic column inhomogeneous.Independent place of chromatographic column in High Performance Liquid Chromatography post constant temperature oven in different length and different posts footpath also can be caused the inconsistent of these chromatographic column working temperatures.In addition, in the high performance liquid chromatography testing process, mobile phase ceaselessly flows in chromatographic system, and this will make the temperature of chromatographic column mobile phase porch and the temperature in exit produce certain temperature difference.Above-mentioned these cause inconsistent, the unstable and inhomogeneous factor of chromatographic column working temperature can affect accuracy and the repeatability of multidimensional efficient liquid phase chromatographic analysis.
Summary of the invention
The temperature homogenizer that the object of the present invention is to provide a kind of many root chromatogram columns rational in infrastructure, that keep many different lengths and consistent, the stable and uniform different length of post chromatographic column working temperature directly and post footpath that in High Performance Liquid Chromatography post constant temperature oven, use to be integrated.
Technical solution of the present invention is:
The temperature homogenizer that many root chromatogram columns in a kind of different length and post footpath are integrated, it is characterized in that: comprise a metal heat-conducting piece, lower metal heat-conducting piece, upper slice metal heat-conducting piece, lower metal heat-conducting piece pass through hinge links, on upper slice metal heat-conducting piece, lower metal heat-conducting piece many upper semi-circle shape chromatographic column grooves and many lower semi-circular chromatographic column grooves are set respectively, and identical, the corresponding setting in position of quantity of upper semi-circle shape chromatographic column groove and lower semi-circular chromatographic column groove; In every upper semi-circle shape chromatographic column groove and lower semi-circular chromatographic column groove, place respectively one or more groups inner metal sleeve pipe, the total length of inner metal sleeve pipe equals by the length of obvolvent chromatographic column cylinder, one group of metal jacket pipe identical with this group inner metal sleeve length of tube of every group of outer suit of inner metal sleeve pipe; Every group of inner metal sleeve pipe is by first identical circular inner metal sleeve pipe of a pair of length, lower semi-circular inner metal sleeve pipe forms, every group of metal jacket pipe is by first identical circular metal jacket pipe of a pair of length, the lower semi-circular metal jacket pipe forms, first circular inner metal sleeve pipe, the external arc of lower semi-circular inner metal sleeve pipe respectively with first circular metal jacket pipe, the interior circular arc of lower semi-circular metal jacket pipe coincide, first circular metal jacket pipe, the external arc of lower semi-circular metal jacket pipe respectively with upper semi-circle shape chromatographic column groove, the interior circular arc of lower semi-circular chromatographic column groove coincide; The inner metal sleeve pipe is the form of choice for use; When the external diameter of the internal diameter of metal jacket pipe and the chromatographic column cylinder that needs to place is identical, only use metal jacket pipe; During the external diameter of the chromatographic column cylinder of placing greater than needs when the internal diameter of metal jacket pipe, increase and use the inner metal sleeve pipe; The external diameter of metal jacket pipe is more than or equal to the external diameter of chromatographic column column cap; The length of upper semi-circle shape chromatographic column groove and lower semi-circular chromatographic column groove is greater than chromatogram column length; The metal jacket pipe total length that one or more groups metal jacket pipe forms in every upper semi-circle shape chromatographic column groove and the lower semi-circular chromatographic column groove need to equal the length of the chromatographic column cylinder placed.
The identical length of upper slice metal heat-conducting piece, lower metal heat-conducting piece, many upper semi-circle shape chromatographic column grooves, many lower semi-circular chromatographic column grooves with.
Set temperature homogenizer upper slice has contact with on upper metal heat-conducting piece, and sheet has contact with post under set temperature homogenizer on the lower metal heat-conducting piece, lower have contact with post and upper slice have contact with and be used.
Described chromatographic column comprises the cylinder of chromatographic column centre and the column cap part at chromatographic column two ends.
The present invention is rational in infrastructure, and its main structure is with many metal heat-conducting piece and inside and outside semicircle metal sleeves of chromatographic column to semicircle chromatographic column groove that can obvolvent.In the test of multidimensional efficient liquid phase chromatographic analysis, we use the semicircle metal jacket pipe of the chromatographic column of this temperature homogenizer, just can allow the chromatographic column of different length place same chromatogram column temperature homogenizer.We add with the semicircle inner metal sleeve pipe of the chromatographic column of this temperature homogenizer, just can allow the chromatographic column in different posts footpath also can place same chromatogram column temperature homogenizer.As seen, by the semicircle metal inner and outer sleeve pipe of the chromatographic column that is combined with this temperature homogenizer, just can make different length place same chromatogram column temperature homogenizer with many root chromatogram columns in different posts footpath, and guarantee the cylinder of chromatographic column and the semicircle inner metal sleeve pipe of chromatographic column or outer tube, the close contact of the semicircle inner metal sleeve pipe of chromatographic column and outer tube, semicircle metal jacket pipe and temperature homogenizer upper slice, lower metal heat-conducting piece semicircle chromatographic column groove.Thereby make many different lengths combine closely in aggregates with cylinder and this temperature homogenizer metal heat-conducting piece of the chromatographic column in different posts footpath.
When the temperature homogenizer that is integrated when many root chromatogram columns in above-mentioned different length and post footpath merges use with High Performance Liquid Chromatography post constant temperature oven, the thermal conductive resin of its metal of temperature homogenizer that is integrated because of many root chromatogram columns in different length and post footpath and with the close contact of High Performance Liquid Chromatography post constant temperature oven, can promote high performance liquid chromatograph constant temperature oven heat transfer efficiency, shorten its thermally equilibrated time, and keep the consistent, stable and even of chromatographic column working temperature.
This temperature homogenizer of the present invention can help to overcome the limitation of existing High Performance Liquid Chromatography post constant temperature oven, keeps the consistent, stable and even of many different lengths and different posts footpath chromatographic column working temperature.When using this temperature homogenizer, we according to the post footpath of chromatographic column, determine whether to use inner sleeve first.As the chromatographic column external diameter of selecting is less than the outer tube internal diameter, is a section of one group of obvolvent chromatographic column cylinder with two inner sleeves first just, with the cylinders of many group inner sleeves with each group inner sleeve next whole chromatographic column of tight obvolvent of mode from beginning to end.The many groups of the outer again obvolvent of inner sleeve outer tube from beginning to end.As the chromatographic column external diameter of selecting equals the internal diameter of outer tube, just directly with the cylinders of many group outer tubes with each group outer tube next whole chromatographic column of tight obvolvent of mode from beginning to end.After the cylinder of chromatographic column was complete by inner sleeve+outer tube or outer tube obvolvent, the column cap of chromatographic column was stayed outside the two ends of inside and outside sleeve pipe of obvolvent chromatographic column cylinder not by obvolvent, and inner sleeve is equated with the total length of outer tube.Because the external diameter of outer tube is identical with the diameter of the fast semicircle chromatographic column groove of temperature homogenizer metal heat-conducting, and more than or equal to the external diameter of chromatographic column column cap, the column cap of chromatographic column just can not affect the heat conduction upper slice of temperature homogenizer, lower obvolvent.And form when using inner sleeve the close contact of the semicircle chromatographic column groove of chromatographic column cylinder and inner sleeve, inner sleeve and outer tube, outer tube and temperature homogenizer.When not using inner sleeve, the close contact of the semicircle chromatographic column groove of chromatographic column cylinder and outer tube, outer tube and temperature homogenizer.
So, the chromatographic column (comprising the column cap part) in different length and different posts footpath can both place by the temperature homogenizer heat conduction upper slice of obvolvent, lower each cylindrical space that obvolvent forms, and the difference that the different length chromatographic column is placed in the cylindrical space only is the length of their two ends redundant spaces in each cylindrical space.Whether the difference of the chromatographic column in different posts footpath just is to add uses inner sleeve.It is in aggregates that final result is that these chromatographic column cylinders can both pass through chromatographic column+inner sleeve+outer tube or chromatographic column+outer tube and metal heat-conducting piece close contact.
When the temperature homogenizer that is integrated when many root chromatogram columns in different length of the present invention and post footpath merges use with High Performance Liquid Chromatography post constant temperature oven, because of the thermal conductive resin of this its metal of temperature homogenizer and with the close contact of high performance liquid chromatograph constant temperature oven, can eliminate above-mentioned independent use High Performance Liquid Chromatography post constant temperature oven keep the chromatographic column working temperature consistent, stable and evenly aspect limitation, improve the efficient of multidimensional efficient liquid phase chromatographic analysis.
Description of drawings
The invention will be further described below in conjunction with drawings and Examples.
Fig. 1 is the structural representation of one embodiment of the invention.
Fig. 2 is the semicircle metal jacket pipe schematic perspective view of chromatographic column.
Fig. 3 is the semicircle inner metal sleeve pipe of chromatographic column schematic perspective view.
Among the figure: (the temperature homogenizer that many root chromatogram columns in different length and post footpath are integrated is hereinafter to be referred as the temperature homogenizer; The semicircle metal jacket pipe of chromatographic column is hereinafter to be referred as outer tube; The semicircle inner metal sleeve pipe of chromatographic column is hereinafter to be referred as inner sleeve)
1. temperature homogenizer upper slice has contact with: have contact with post with sheet under the temperature homogenizer and be used, obvolvent temperature homogenizer.
2. upper slice metal heat-conducting piece of temperature homogenizer: quick conductive, evenly by the temperature of obvolvent chromatographic column.
3. first of the temperature homogenizer upper slice semicircle chromatographic column groove: when the obvolvent of temperature homogenizer, form the cylindrical space of placing by the first chromatographic column of outer tube (when the internal diameter of the external diameter of chromatographic column cylinder and outer tube is identical) or outer tube+inner sleeve (when the external diameter of chromatographic column cylinder during less than the internal diameter of outer tube) obvolvent with the semicircle chromatographic column groove of first time sheet of temperature homogenizer.
4. second of the temperature homogenizer upper slice semicircle chromatographic column groove: when the obvolvent of temperature homogenizer, form the cylindrical space of placing by the second chromatographic column of outer tube (when the internal diameter of the external diameter of chromatographic column cylinder and outer tube is identical) or outer tube+inner sleeve (when the external diameter of chromatographic column cylinder during less than the internal diameter of outer tube) obvolvent with the semicircle chromatographic column groove of second time sheet of temperature homogenizer.
5. the 3rd of the temperature homogenizer the upper slice semicircle chromatographic column groove: when the obvolvent of temperature homogenizer, form the cylindrical space of placing by the 3rd chromatographic column of outer tube (when the internal diameter of the external diameter of chromatographic column cylinder and outer tube is identical) or outer tube+inner sleeve (when the external diameter of chromatographic column cylinder during less than the internal diameter of outer tube) obvolvent with the semicircle chromatographic column groove of the 3rd time sheet of temperature homogenizer.
6. the heat conduction upper slice of temperature homogenizer, lower link hinge: chain jointing temp homogenizer heat conduction upper slice, lower, and make it free-open-close.
7. lower metal heat-conducting piece of temperature homogenizer: quick conductive, evenly by the temperature of obvolvent chromatographic column.
8. the semicircle chromatographic column groove of first of the temperature homogenizer time sheet: when the obvolvent of temperature homogenizer, form the cylindrical space of placing by the first chromatographic column of outer tube (when the internal diameter of the external diameter of chromatographic column cylinder and outer tube is identical) or outer tube+inner sleeve (when the external diameter of chromatographic column cylinder during less than the internal diameter of outer tube) obvolvent with the first upper slice semicircle chromatographic column groove of temperature homogenizer.
9. the semicircle chromatographic column groove of second of the temperature homogenizer time sheet: when the obvolvent of temperature homogenizer, form the cylindrical space of placing by the second chromatographic column of outer tube (when the internal diameter of the external diameter of chromatographic column cylinder and outer tube is identical) or outer tube+inner sleeve (when the external diameter of chromatographic column cylinder during less than the internal diameter of outer tube) obvolvent with the second upper slice semicircle chromatographic column groove of temperature homogenizer.
10. the semicircle chromatographic column groove of the 3rd of the temperature homogenizer the time sheet: when the obvolvent of temperature homogenizer, form the cylindrical space of placing by the 3rd chromatographic column of outer tube (when the internal diameter of the external diameter of chromatographic column cylinder and outer tube is identical) or outer tube+inner sleeve (when the external diameter of chromatographic column cylinder during less than the internal diameter of outer tube) obvolvent with the 3rd upper slice semicircle chromatographic column groove of temperature homogenizer.
11. lower of the temperature homogenizer is had contact with post: have contact with temperature homogenizer upper slice and be used obvolvent temperature homogenizer.
12. outer tube: use two outer tubes to be one group and form the cylindrical space, with the tight obvolvent inner sleeve of outer sleeve inner wall (when the external diameter of chromatographic column cylinder during less than the internal diameter of outer tube) or chromatographic column cylinder (when the internal diameter of the external diameter of chromatographic column cylinder and outer tube is identical), and make outer tube outer wall and temperature homogenizer upper slice, the lower semicircle chromatographic column groove of metal heat-conducting piece form close contact.
13. inner sleeve: when chromatographic column cylinder external diameter during less than the outer tube internal diameter, use first the two tight obvolvent chromatographic column of inner sleeve cylinders, inner sleeve adds two outer tubes of bag outward again, and makes the semicircle chromatographic column groove of inner sleeve and outer tube, outer tube and temperature homogenizer form close contact.
Embodiment
The temperature homogenizer that many root chromatogram columns in a kind of different length and post footpath are integrated, comprise a metal heat-conducting piece, lower metal heat-conducting piece, upper slice metal heat-conducting piece, lower metal heat-conducting piece pass through hinge links, on upper slice metal heat-conducting piece, lower metal heat-conducting piece many upper semi-circle shape chromatographic column grooves and many lower semi-circular chromatographic column grooves are set respectively, and identical, the corresponding setting in position of quantity of upper semi-circle shape chromatographic column groove and lower semi-circular chromatographic column groove; In every upper semi-circle shape chromatographic column groove and lower semi-circular chromatographic column groove, place respectively one or more groups inner metal sleeve pipe, the total length of inner metal sleeve pipe equals by the length of obvolvent chromatographic column cylinder, one group of metal jacket pipe identical with this group inner metal sleeve length of tube of every group of outer suit of inner metal sleeve pipe; Every group of inner metal sleeve pipe is by first identical circular inner metal sleeve pipe of a pair of length, lower semi-circular inner metal sleeve pipe forms, every group of metal jacket pipe is by first identical circular metal jacket pipe of a pair of length, the lower semi-circular metal jacket pipe forms, first circular inner metal sleeve pipe, the external arc of lower semi-circular inner metal sleeve pipe respectively with first circular metal jacket pipe, the interior circular arc of lower semi-circular metal jacket pipe coincide, first circular metal jacket pipe, the external arc of lower semi-circular metal jacket pipe respectively with upper semi-circle shape chromatographic column groove, the interior circular arc of lower semi-circular chromatographic column groove coincide; The inner metal sleeve pipe is the form of choice for use; When the external diameter of the internal diameter of metal jacket pipe and the chromatographic column cylinder that needs to place is identical, only use metal jacket pipe; During the external diameter of the chromatographic column cylinder of placing greater than needs when the internal diameter of metal jacket pipe, increase and use the inner metal sleeve pipe; The external diameter of metal jacket pipe is more than or equal to the external diameter of chromatographic column column cap; The length of upper semi-circle shape chromatographic column groove and lower semi-circular chromatographic column groove is greater than chromatogram column length; The metal jacket pipe total length that one or more groups metal jacket pipe forms in every upper semi-circle shape chromatographic column groove and the lower semi-circular chromatographic column groove need to equal the length of the chromatographic column cylinder placed.
The identical length of upper slice metal heat-conducting piece, lower metal heat-conducting piece, many upper semi-circle shape chromatographic column grooves, many lower semi-circular chromatographic column grooves with.
Set temperature homogenizer upper slice has contact with on upper metal heat-conducting piece, and sheet has contact with post under set temperature homogenizer on the lower metal heat-conducting piece, lower have contact with post and upper slice have contact with and be used.
Described chromatographic column comprises the cylinder of chromatographic column centre and the column cap part at chromatographic column two ends.
(the temperature homogenizer that many root chromatogram columns in different length and post footpath are integrated is hereinafter to be referred as the temperature homogenizer for the course of work of this equipment; The semicircle metal jacket pipe of chromatographic column is hereinafter to be referred as outer tube; The semicircle inner metal sleeve pipe of chromatographic column is hereinafter to be referred as inner sleeve)
(1) connects chromatographic column
According to requirement of experiment choose different length and post footpath chromatographic column, with selected the first chromatographic column, turn on the post lid at chromatographic column two ends, chromatographic column is linked to each other with the mobile phase inlet/outlet pipe respectively according to the direction of arrow, with special spanner outlet and the porch of chromatographic column are tightened.Connect successively the second chromatographic column and the 3rd chromatographic column with above-mentioned identical method.
(2) directly select to use outer tube or outer tube+inner sleeve obvolvent chromatographic column cylinder according to length and the post of chromatographic column;
When the internal diameter of the external diameter of chromatographic column cylinder and outer tube is identical, take two outer tubes (12) as one group, length according to the chromatographic column cylinder, be one group of a section of forming cylindrical space obvolvent chromatographic column cylinder with two outer tubes, come the cylinder of the whole chromatographic column of tight obvolvent in each group outer tube mode from beginning to end with many groups outer tube, the column cap that only stays chromatographic column outside the outer tube two ends of obvolvent not by obvolvent, the external diameter that makes outer tube is identical with the diameter of the semicircle chromatographic column groove of temperature homogenizer, and more than or equal to the external diameter of chromatographic column column cap.
When the external diameter of chromatographic column cylinder during less than the outer tube internal diameter, first take two inner sleeves (13) as one group, length according to the chromatographic column cylinder, be one group with two inner sleeves and form the cylindrical space, closely one section of obvolvent chromatographic column cylinder, organize the cylinder that inner sleeve mode from beginning to end is come the whole chromatographic column of tight obvolvent with many group inner sleeves with each.Inner sleeve adds the bag outer tube by above-mentioned method outward again, and inner sleeve is equated with the total length of outer tube, the column cap that only stays chromatographic column outside the inside and outside sleeve pipe two ends of obvolvent not by obvolvent.And the external diameter of inner sleeve is equated with the internal diameter of outer tube, the chromatographic column semi-circular groove equal diameters of the external diameter of outer tube and temperature homogenizer.
(3) chromatographic column that obvolvent is complete is installed in the temperature homogenizer
After temperature homogenizer upper slice had contact with (1) sheet under the temperature homogenizer and have contact with post (11) and remove, upwards open temperature homogenizer upper slice metal heat-conducting piece (2), to connect successively and put into outer tube or good the first chromatographic column (the column cap part that comprises chromatographic column) of outer tube+inner sleeve obvolvent the semicircle chromatographic column groove (8) of first time sheet of temperature homogenizer, with connecting and putting into the semicircle chromatographic column groove (9) of second time sheet of temperature homogenizer with outer tube or good the second chromatographic column (the column cap part that comprises chromatographic column) of outer tube+inner sleeve obvolvent, will connect and put into outer tube or good the 3rd chromatographic column (the column cap part that comprises chromatographic column) of outer tube+inner sleeve obvolvent the semicircle chromatographic column groove (10) of the 3rd time sheet of temperature homogenizer.Move down temperature homogenizer upper slice metal heat-conducting piece (2), make it with the temperature homogenizer under sheet metal heat-conducting piece (7) closure.First upper slice semicircle chromatographic column groove (3) of formation temperature homogenizer and the semicircle chromatographic column groove (8) of first time sheet of temperature homogenizer, second upper slice semicircle chromatographic column groove (4) of temperature homogenizer and the semicircle chromatographic column groove (9) of second time sheet of temperature homogenizer, the 3rd upper slice semicircle chromatographic column groove (5) of temperature homogenizer and the semicircle chromatographic column groove of the 3rd time sheet (10) of temperature homogenizer be 3 cylindrical spaces of obvolvent respectively, allow and placed by the chromatographic column of obvolvent (the column cap part that comprises the chromatographic column two ends), lower metal heat-conducting piece is in the middle of the cylindrical of obvolvent, in the length of chromatographic column during less than the semicircle chromatographic column slot length of temperature homogenizer, chromatographic column by obvolvent is placed between two parties, and on average reserve the remaining space at two ends, when guaranteeing to use inner sleeve simultaneously, chromatographic column cylinder and inner sleeve, inner sleeve and outer tube, the close contact of the semicircle chromatographic column groove of outer tube and temperature homogenizer.When only using outer tube, the close contact of the semicircle chromatographic column groove of chromatographic column cylinder and outer tube, outer tube and temperature homogenizer.Thereby make the cylinder of above-mentioned many root chromatogram columns and temperature homogenizer metal heat-conducting piece combine closely in aggregates.Mobile temperature homogenizer upper slice has contact with (1), makes it to catch on that sheet has contact with post (11) under the temperature homogenizer, locks by obvolvent temperature homogenizer.
(4) the temperature homogenizer is put into High Performance Liquid Chromatography post constant temperature oven
After finishing above-mentioned action, the bottom of sheet metal heat-conducting piece (7) under the chromatogram column temperature homogenizer of this many root chromatogram columns of obvolvent one is placed on the bottom of High Performance Liquid Chromatography post constant temperature oven, loam cake and the fastening of the High Performance Liquid Chromatography of closing post constant temperature oven.The top of the temperature homogenizer upper slice metal heat-conducting piece (2) of many root chromatogram columns one is contacted with the constant temperature case lid.
(5) start detects
Then open routinely high performance liquid chromatograph, working temperature and other parameter of chromatographic column is set, behind the preheating certain hour, begin to detect.
Claims (3)
1. the temperature homogenizer that is integrated of many root chromatogram columns in a different length and post footpath, it is characterized in that: comprise a metal heat-conducting piece, lower metal heat-conducting piece, upper slice metal heat-conducting piece, lower metal heat-conducting piece pass through hinge links, on upper slice metal heat-conducting piece, lower metal heat-conducting piece many upper semi-circle shape chromatographic column grooves and many lower semi-circular chromatographic column grooves are set respectively, and identical, the corresponding setting in position of quantity of upper semi-circle shape chromatographic column groove and lower semi-circular chromatographic column groove; In every upper semi-circle shape chromatographic column groove and lower semi-circular chromatographic column groove, place respectively one or more groups inner metal sleeve pipe, the total length of inner metal sleeve pipe equals by the length of obvolvent chromatographic column cylinder, one group of metal jacket pipe identical with this group inner metal sleeve length of tube of every group of outer suit of inner metal sleeve pipe; Every group of inner metal sleeve pipe is by first identical circular inner metal sleeve pipe of a pair of length, lower semi-circular inner metal sleeve pipe forms, every group of metal jacket pipe is by first identical circular metal jacket pipe of a pair of length, the lower semi-circular metal jacket pipe forms, first circular inner metal sleeve pipe, the external arc of lower semi-circular inner metal sleeve pipe respectively with first circular metal jacket pipe, the interior circular arc of lower semi-circular metal jacket pipe coincide, first circular metal jacket pipe, the external arc of lower semi-circular metal jacket pipe respectively with upper semi-circle shape chromatographic column groove, the interior circular arc of lower semi-circular chromatographic column groove coincide; The inner metal sleeve pipe is the form of choice for use; When the external diameter of the internal diameter of metal jacket pipe and the chromatographic column cylinder that needs to place is identical, only use metal jacket pipe; During the external diameter of the chromatographic column cylinder of placing greater than needs when the internal diameter of metal jacket pipe, increase and use the inner metal sleeve pipe; The external diameter of metal jacket pipe is more than or equal to the external diameter of chromatographic column column cap; The length of upper semi-circle shape chromatographic column groove and lower semi-circular chromatographic column groove is greater than chromatogram column length; The metal jacket pipe total length that one or more groups metal jacket pipe forms in every upper semi-circle shape chromatographic column groove and the lower semi-circular chromatographic column groove need to equal the length of the chromatographic column cylinder placed.
2. the temperature homogenizer that is integrated of many root chromatogram columns in different length according to claim 1 and post footpath is characterized in that: the identical length of upper slice metal heat-conducting piece, lower metal heat-conducting piece, many upper semi-circle shape chromatographic column grooves, many lower semi-circular chromatographic column grooves with.
3. the temperature homogenizer that is integrated of many root chromatogram columns in different length according to claim 1 and 2 and post footpath, it is characterized in that: set temperature homogenizer upper slice has contact with on upper metal heat-conducting piece, sheet has contact with post under set temperature homogenizer on the lower metal heat-conducting piece, lower have contact with post and upper slice have contact with and be used.
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| CN201210403661XA CN102901785B (en) | 2012-10-22 | 2012-10-22 | Temperature uniformizer integrating multiple chromatographic columns with different lengths and diameters |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104634905A (en) * | 2015-01-14 | 2015-05-20 | 杭州纽蓝科技有限公司 | Small-sized all-in-one liquid chromatograph with on-line analysis function |
| WO2021120609A1 (en) * | 2020-07-13 | 2021-06-24 | 南通大学 | Chromatography column temperature equalizer for ensuring temperature uniformity |
| WO2021120610A1 (en) * | 2020-07-13 | 2021-06-24 | 南通大学 | Highly-adjustable chromatography column temperature equalizer |
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| US5983710A (en) * | 1997-11-24 | 1999-11-16 | Brinkman Instruments, Inc. | Modular chromatography column heating system |
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| CN201844999U (en) * | 2010-11-03 | 2011-05-25 | 上海科油石油仪器制造有限公司 | Gas chromatographic thermostat |
| CN202033335U (en) * | 2011-03-04 | 2011-11-09 | 青岛盛瀚色谱技术有限公司 | Thermostat of ion chromatography conductivity detector |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104634905A (en) * | 2015-01-14 | 2015-05-20 | 杭州纽蓝科技有限公司 | Small-sized all-in-one liquid chromatograph with on-line analysis function |
| WO2021120609A1 (en) * | 2020-07-13 | 2021-06-24 | 南通大学 | Chromatography column temperature equalizer for ensuring temperature uniformity |
| WO2021120610A1 (en) * | 2020-07-13 | 2021-06-24 | 南通大学 | Highly-adjustable chromatography column temperature equalizer |
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| CN102901785B (en) | 2013-12-04 |
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