CN102885844B - Application of Houttuynoid B in medicine for treating tongue cancer - Google Patents
Application of Houttuynoid B in medicine for treating tongue cancer Download PDFInfo
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- CN102885844B CN102885844B CN201210417500.6A CN201210417500A CN102885844B CN 102885844 B CN102885844 B CN 102885844B CN 201210417500 A CN201210417500 A CN 201210417500A CN 102885844 B CN102885844 B CN 102885844B
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- houttuynoid
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Abstract
The invention discloses an application of Houttuynoid B in preparation for a medicine for treating tongue cancer, belonging to the technical field of medicine new applications. According to the invention, in-vitro MTT (3-4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) anti-tumour activity evaluation finds that Houttuynoid B also has a remarkable inhibition effect on the growths of human tongue cancer cell strains Tca8113 and T6. Thus, Houttuynoid B can be used for preparing the medicine for treating the tongue cancer, and has a good development and application prospect. The application of the Houttuynoid B in preparation for the medicine for treating the tongue cancer is initially disclosed; and Houttuynoid B has a brand new framework type and an unexpected inhibition activity on tongue cancer cells.
Description
Technical field
The present invention relates to the new purposes of compound H outtuynoid B, relate in particular to the application of Houttuynoid B in the anti-carcinoma of tongue medicine of preparation.
Technical background
Cancer is to one of disease of human life's health hazard maximum, has a large amount of people to die from cancer every year.The research and development of cancer therapy drug are the focuses of study of pharmacy always.In antitumor drug, have 74% to be natural product or derivatives thereof, if paclitaxel and derivant thereof are exactly the reasonable antitumor drug of effect clinically at present.Therefore, from natural product, find anticancer compound or lead compound has great importance.
The compound H outtuynoid B the present invention relates to is one and within 2012, delivers (Chen, S. D. et al., 2012. Houttuynoid B_E, Anti-Herpes Simplex Virus Active Flavonoids with Novel Skeletons from Houttuynia cordata. Organic Letters 14 (7), 1772 – 1775.) New skeleton compound, this compound has brand-new framework types, current purposes only relates to anti-herpes simplex virus activity (Chen, S. D. et al., 2012. Houttuynoid B_E, Anti-Herpes Simplex Virus Active Flavonoids with Novel Skeletons from Houttuynia cordata. Organic Letters 14 (7), 1772 – 1775.), purposes for the Houttuynoid B the present invention relates in preparation treatment carcinoma of tongue medicine belongs to open first, because framework types belongs to brand-new framework types, and its inhibition for Human Tongue Carcinoma Lines is active unexpectedly strong, there is not the possibility that is provided any enlightenment by other compounds, possesses outstanding substantive distinguishing features, control for carcinoma of tongue simultaneously obviously has significant progress.
Summary of the invention
The invention provides the application of compound H outtuynoid B in preparing antitumor drug.
The present invention adopts following technical scheme: the application of Houttuynoid B in the anti-carcinoma of tongue medicine of preparation, and the structural formula of Houttuynoid B is as shown in formula I:
The present invention finds by the evaluation of external MTT anti-tumor activity, and Houttuynoid B also has significant inhibitory action to the growth of people's Human Tongue Carcinoma Lines strain Tca8113 and T6, suppresses the IC of this 2 strain Growth of Cells
50value is respectively 2.13 ± 0.21 μ M and 3.19 ± 0.14 μ M.Therefore, Houttuynoid B can, for the preparation of anti-carcinoma of tongue medicine, have good development prospect.
The purposes of the Houttuynoid B the present invention relates in preparation treatment carcinoma of tongue medicine belongs to open first, because framework types belongs to brand-new framework types, and its inhibition for Human Tongue Carcinoma Lines is active unexpectedly strong, there is not the possibility that is provided any enlightenment by other compounds, possess outstanding substantive distinguishing features, the control for carcinoma of tongue simultaneously obviously has significant progress.
The present invention is further detailed explanation by the following examples, but protection scope of the present invention is not subject to any restriction of specific embodiment, but be limited by claim.
The specific embodiment
The preparation method of compound H outtuynoid B involved in the present invention is referring to document (Chen, S. D. et al., 2012. Houttuynoid B_E, Anti-Herpes Simplex Virus Active Flavonoids with Novel Skeletons from Houttuynia cordata. Organic Letters 14 (7), 1772 – 1775.).
The present invention is further detailed explanation by the following examples, but protection scope of the present invention is not subject to any restriction of specific embodiment, but be limited by claim.
Embodiment 1: the preparation of compound H outtuynoid B tablet involved in the present invention:
Get 20 and digest compound Houttuynoid B, add 180 grams of conventional adjuvants preparing tablet, mix, conventional tablet machine is made 1000.
Embodiment 2: the preparation of compound H outtuynoid B capsule involved in the present invention:
Get 20 and digest compound Houttuynoid B, add the conventional adjuvant of preparing capsule as 180 grams of starch, mix, encapsulatedly make 1000.
Below by pharmacodynamic experiment, further illustrate its pharmaceutically active.
Experimental example: adopt the growth inhibited effect of mtt assay assessing compound Houttuynoid B to the strain of people's Human Tongue Carcinoma Lines
1. method: the cell in growth logarithmic (log) phase: people's Human Tongue Carcinoma Lines strain Tca8113 and T6(buy from Chinese Academy of Sciences's cell bank) with 1.5 * 10
4concentration kind is in 96 orifice plates.After cell culture 24 h are adherent, suck original culture medium.Test is divided into blank group, drug treating group.Blank group is changed 1640 culture medium containing 10% hyclone; It is 100 μ M that drug treating group is changed containing concentration, 50 μ M, 10 μ M, 1 μ M, 0.1 μ M, the culture medium of the Houttuynoid B of 0.01 μ M and 0.001 μ M.Cultivate after 48 h, add the MTT of concentration 5 mg/mL, continue to be put in CO
2incubator is cultivated 4 h, then along culture fluid top, suck 100 μ L supernatants, add 100 μ L DMSO, 10 min are placed in dark place, utilize microplate reader (Sunrise company product) to measure light absorption value (wavelength 570nm), and calculating cell survival situation according to light absorption value, 6 repeating holes are established in each processing.Cell survival rate (%)=Δ OD
drug treating/ Δ OD
blank* 100.
2. result: Houttuynoid B has significant inhibitory action to the growth of people's Human Tongue Carcinoma Lines strain Tca8113 and T6.This compound suppresses the IC of people Human Tongue Carcinoma Lines strain Tca8113 and T6 growth
50value is respectively: 2.13 ± 0.21 μ M and 3.19 ± 0.14 μ M.
By above-described embodiment, shown, Houttuynoid B of the present invention has good inhibitory action to the growth of people's Human Tongue Carcinoma Lines strain Tca8113 and T6.Prove thus, it is active that Houttuynoid B of the present invention has anti-carcinoma of tongue, can be for the preparation of anti-carcinoma of tongue medicine.
Claims (1)
- The application of 1.Houttuynoid B in preparation treatment carcinoma of tongue medicine, described compound H outtuynoid B structure as formula Ishown in:
formula I.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210417500.6A CN102885844B (en) | 2012-10-27 | 2012-10-27 | Application of Houttuynoid B in medicine for treating tongue cancer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210417500.6A CN102885844B (en) | 2012-10-27 | 2012-10-27 | Application of Houttuynoid B in medicine for treating tongue cancer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102885844A CN102885844A (en) | 2013-01-23 |
| CN102885844B true CN102885844B (en) | 2014-04-16 |
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ID=47529663
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210417500.6A Active CN102885844B (en) | 2012-10-27 | 2012-10-27 | Application of Houttuynoid B in medicine for treating tongue cancer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102885844B (en) |
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2012
- 2012-10-27 CN CN201210417500.6A patent/CN102885844B/en active Active
Non-Patent Citations (2)
| Title |
|---|
| Shao-Dan Chen,等.Houttuynoids A-E, Anti-Herpes Simplex Virus Active Flavonoids with Novel Skeletons from Houttuynia cordata.《Organic Letters》.2012,第14卷(第7期),1772-1775. * |
| Shao-DanChen，等.HouttuynoidsA-E Anti-Herpes Simplex Virus Active Flavonoids with Novel Skeletons from Houttuynia cordata.《Organic Letters》.2012 |
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| Publication number | Publication date |
|---|---|
| CN102885844A (en) | 2013-01-23 |
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