CN102861078B - Application of Houttuynoid D in medicine for treating prostatic cancer - Google Patents
Application of Houttuynoid D in medicine for treating prostatic cancer Download PDFInfo
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- CN102861078B CN102861078B CN201210418640.5A CN201210418640A CN102861078B CN 102861078 B CN102861078 B CN 102861078B CN 201210418640 A CN201210418640 A CN 201210418640A CN 102861078 B CN102861078 B CN 102861078B
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Abstract
The invention discloses application of Houttuynoid D in preparation of a medicine for treating prostatic cancer, belonging to the technical field of new application of medicine. The invention discovers that Houttuynoid D has the remarkable inhibitory effects on the growth of human prostatic cancer cell strains, including ALVA, gpc-1, 9L-B and Du145 through the evaluation of in-vitro MTT antineoplastic activity. Therefore, Houttuynoid D can be used for preparing the anti-prostatic cancer medicine and has good development and application prospects. The application of Houttuynoid D in preparation of the medicine for treating prostatic cancer provided by the invention is disclosed firstly. The skeleton type belongs to new skeleton type, and the inhibitory activity of Houttuynoid D on prostatic cancer cells is unexpectedly strong.
Description
Technical field
The present invention relates to the new purposes of compound H outtuynoid D, relate in particular to the application of Houttuynoid D in preparing antiprostate cancer.
Technical background
Cancer is to one of disease of human life's health hazard maximum, has a large amount of people to die from cancer every year.The research and development of cancer therapy drug are the focuses of study of pharmacy always.In antitumor drug, have 74% to be natural product or derivatives thereof, if paclitaxel and derivant thereof are exactly the reasonable antitumor drug of effect clinically at present.Therefore, from natural product, find anticancer compound or lead compound has great importance.
The compound H outtuynoid D the present invention relates to is one and within 2012, delivers (Chen, S. D. et al., 2012. Houttuynoid D_E, Anti-Herpes Simplex Virus Active Flavonoids with Novel Skeletons from Houttuynia cordata. Organic Letters 14 (7), 1772 – 1775.) New skeleton compound, this compound has brand-new framework types, current purposes only relates to anti-herpes simplex virus activity (Chen, S. D. et al., 2012. Houttuynoid D_E, Anti-Herpes Simplex Virus Active Flavonoids with Novel Skeletons from Houttuynia cordata. Organic Letters 14 (7), 1772 – 1775.), purposes for the Houttuynoid D the present invention relates in preparation treatment carcinoma of prostate medicine belongs to open first, because framework types belongs to brand-new framework types, and its inhibition for prostate gland cancer cell is active unexpectedly strong, there is not the possibility that is provided any enlightenment by other compounds, possesses outstanding substantive distinguishing features, control for carcinoma of prostate simultaneously obviously has significant progress.
Summary of the invention
The invention provides the application of compound H outtuynoid D in preparing antitumor drug.
The present invention adopts following technical scheme: the application of Houttuynoid D in preparing antiprostate cancer, and the structural formula of Houttuynoid D is as shown in formula I:
The present invention finds by the evaluation of external MTT anti-tumor activity, and Houttuynoid D also has significant inhibitory action to the growth of human prostate cancer cell line ALVA, gpc-1,9L-B and Du145, suppresses the IC of this 4 strain Growth of Cells
50value is respectively 0.19 ± 0.03 μ M, 0.16 ± 0.06 μ M, 1.13 ± 0.15 μ M and 2.14 ± 0.36 μ M.Therefore, Houttuynoid D can, for the preparation of antiprostate cancer, have good development prospect.
The purposes of the Houttuynoid D the present invention relates in preparation treatment carcinoma of prostate medicine belongs to open first, because framework types belongs to brand-new framework types, and its inhibition for prostate gland cancer cell is active unexpectedly strong, there is not the possibility that is provided any enlightenment by other compounds, possess outstanding substantive distinguishing features, the control for carcinoma of prostate simultaneously obviously has significant progress.
The present invention is further detailed explanation by the following examples, but protection scope of the present invention is not subject to any restriction of specific embodiment, but be limited by claim.
The specific embodiment
The preparation method of compound H outtuynoid D involved in the present invention is referring to document (Chen, S. D. et al., 2012. Houttuynoid D_E, Anti-Herpes Simplex Virus Active Flavonoids with Novel Skeletons from Houttuynia cordata. Organic Letters 14 (7), 1772 – 1775.).
The present invention is further detailed explanation by the following examples, but protection scope of the present invention is not subject to any restriction of specific embodiment, but be limited by claim.
Embodiment 1: the preparation of compound H outtuynoid D tablet involved in the present invention:
Get 20 and digest compound Houttuynoid D, add 180 grams of conventional adjuvants preparing tablet, mix, conventional tablet machine is made 1000.
Embodiment 2: the preparation of compound H outtuynoid D capsule involved in the present invention:
Get 20 and digest compound Houttuynoid D, add the conventional adjuvant of preparing capsule as 180 grams of starch, mix, encapsulatedly make 1000.
Below by pharmacodynamic experiment, further illustrate its pharmaceutically active.
Experimental example: adopt the growth inhibited effect of mtt assay assessing compound Houttuynoid D to human prostate cancer cell line
1. method: the cell in growth logarithmic (log) phase: human prostate cancer cell line ALVA, gpc-1,9L-B and Du145(buy from Chinese Academy of Sciences's cell bank) with 1.5 * 10
4concentration kind is in 96 orifice plates.After cell culture 24 h are adherent, suck original culture medium.Test is divided into blank group, drug treating group.Blank group is changed 1640 culture medium containing 10% hyclone; It is 100 μ M that drug treating group is changed containing concentration, 50 μ M, 10 μ M, 1 μ M, 0.1 μ M, the culture medium of the Houttuynoid D of 0.01 μ M and 0.001 μ M.Cultivate after 48 h, add the MTT of concentration 5mg/mL, continue to be put in CO
2incubator is cultivated 4 h, then along culture fluid top, suck 100 μ L supernatants, add 100 μ L DMSO, 10 min are placed in dark place, utilize microplate reader (Sunrise company product) to measure light absorption value (wavelength 570nm), and calculating cell survival situation according to light absorption value, 6 repeating holes are established in each processing.Cell survival rate (%)=Δ OD
drug treating/ Δ OD
blank* 100.
2. result: Houttuynoid D has significant inhibitory action to the growth of human prostate cancer cell line ALVA, gpc-1,9L-B and Du145.This compound suppresses the IC of human prostate cancer cell line ALVA, gpc-1,9L-B and Du145 growth
50value is respectively: 0.19 ± 0.03 μ M, 0.16 ± 0.06 μ M, 1.13 ± 0.15 μ M and 2.14 ± 0.36 μ M.
By above-described embodiment, shown, Houttuynoid D of the present invention has good inhibitory action to the growth of human prostate cancer cell line ALVA, gpc-1,9L-B and Du145.Prove thus, Houttuynoid D of the present invention has anti-prostate cancer activity, can be for the preparation of antiprostate cancer.
Claims (1)
- The application of 1.Houttuynoid D in preparation treatment carcinoma of prostate medicine, described compound H outtuynoid D structure as formula Ishown in:
formula I.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201210418640.5A CN102861078B (en) | 2012-10-27 | 2012-10-27 | Application of Houttuynoid D in medicine for treating prostatic cancer |
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| CN201210418640.5A CN102861078B (en) | 2012-10-27 | 2012-10-27 | Application of Houttuynoid D in medicine for treating prostatic cancer |
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| CN102861078A CN102861078A (en) | 2013-01-09 |
| CN102861078B true CN102861078B (en) | 2014-04-16 |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1880328A (en) * | 2006-04-30 | 2006-12-20 | 重庆大学 | Preparation method of hyperin and hypericin of Hypericum perforatum |
| CN102697942A (en) * | 2012-01-04 | 2012-10-03 | 陈玉堂 | Medicament capable of killing cancer cells and curing various tumors and cancers and leukemia |
-
2012
- 2012-10-27 CN CN201210418640.5A patent/CN102861078B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1880328A (en) * | 2006-04-30 | 2006-12-20 | 重庆大学 | Preparation method of hyperin and hypericin of Hypericum perforatum |
| CN102697942A (en) * | 2012-01-04 | 2012-10-03 | 陈玉堂 | Medicament capable of killing cancer cells and curing various tumors and cancers and leukemia |
Non-Patent Citations (4)
| Title |
|---|
| Houttuynoids A-E, anti-herpes simplex virus active flavonoids with novel skeletons from houttuynia cordata;Shao-Dan Chen et al;《Organic Letters》;20120313;第14卷(第7期);第1773页 * |
| Shao-DanChenetal.HouttuynoidsA-E anti-herpes simplex virus active flavonoids with novel skeletons from houttuynia cordata.《Organic Letters》.2012 |
| 李爽 等.鱼腥草的有效成分、药理作用及临床应用的研究进展.《沈阳药科大学学报》.1997,第14卷(第2期),第144-146页. |
| 鱼腥草的有效成分、药理作用及临床应用的研究进展;李爽 等;《沈阳药科大学学报》;19970430;第14卷(第2期);第144-146页 * |
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