CN102885305A - Health food composition for enhancing immunity and preparation method thereof - Google Patents
Health food composition for enhancing immunity and preparation method thereof Download PDFInfo
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- CN102885305A CN102885305A CN2012103862025A CN201210386202A CN102885305A CN 102885305 A CN102885305 A CN 102885305A CN 2012103862025 A CN2012103862025 A CN 2012103862025A CN 201210386202 A CN201210386202 A CN 201210386202A CN 102885305 A CN102885305 A CN 102885305A
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Abstract
The invention relates to a health food composition for enhancing immunity and a preparation method of the health food composition. The health food composition is prepared into hard capsules, troches, particles, powder and bag medicinal tea from 145-200 parts by weight of dried sea cucumber powder, 70-130 parts by weight of cervus elaphus Linnaeus powder and 130-180 by weight parts of extract of the fruit of Chinese wolfberry and clinically acceptable auxiliary materials. The health food composition for enhancing immunity is prepared from dried sea cucumber powder, cervus elaphus Linnaeus powder and extract of the fruit of Chinese wolfberry. The three materials are matched to meet the function of enhancing immunity. The product preparation process is simple, and the cost is lowered. The immunity-enhancing function experiments and safety evaluation experiments show that the health food composition has an explicit function of enhancing immunity, is safe without toxic side effects, and can be taken for a long time. The content of functional component of crude polysaccharide of the prepared product is more than 0.5g in every 100g of the prepared product.
Description
Technical field
The present invention relates to a kind of health-care food composition that strengthens immunity and preparation method thereof, belong to the health food technology field.
Background technology
The immunologic function of body is to realize that by the immune system of a complexity it comprises immune organ, immunocyte and immune factor.The normalization of immune existence and function is the stable basic guarantee of body's immunity, any part damaged or unusually all can cause the incomplete or disorderly of immunologic function wherein, thus reduce or the forfeiture immunologic function.Immunologic function is not enough or lowly can produce totally unfavorable impact to body health, and the M ﹠ M of multiple infectious disease or non-infective disease is improved.Cause the low reason of immunity of organism a lot, such as nutrient imbalance, spirit or psychological factor, age increase, endocrinopathy etc.The health of hypoimmunity very easily causes the infection such as bacterium, virus, fungi, so the most direct performance of hypoimmunity is exactly liable to illness.Because of often ill, increased the weight of the consumption of body, thus generally have a delicate constitution, the performances such as malnutritive, One's spirits are drooping, fatigue and weak, appetite reduction, sleep-disorder, sick, have an injection and take medicine the normal potluck that just gets married.Each sickly all will could recover for a long time, and usually repeatedly outbreak.If things go on like this can cause health and intelligence development bad, also easily bring out major disease.
At present, have the health food of numerous enhancing immunity on the market, this product is with animal drugs, autonomic drug, ocean medicine compatibility, the prescription clear efficacy, and compatibility is reasonable.
Summary of the invention
In order to solve the problems of the technologies described above, the invention provides a kind of health-care food composition that strengthens immunity and preparation method thereof.
The invention provides a kind of health-care food composition that strengthens immunity, contain the bulk drug of following weight portion:
Holothurian freeze-dried powder 145～200; Cervus elaphus linnaeus powder 70～130; Fructus lycii P.E 130～180.
Preferably, wherein said health-care food composition, contain the bulk drug of following weight portion:
Holothurian freeze-dried powder 155～180; Cervus elaphus linnaeus powder 90～110; Fructus lycii P.E 140 ~ 170.
Preferably, wherein said health-care food composition, contain the bulk drug of following weight portion:
Holothurian freeze-dried powder 167; Cervus elaphus linnaeus powder 100; Fructus lycii P.E 158.
Preferably, the crude protein content in the wherein said holothurian freeze-dried powder by weight percentage 〉=50%.
Preferably, the thick polyoses content in the wherein said Fructus lycii P.E by weight percentage 〉=6%.
Preferably, wherein said health-care food composition is to make oral formulations; Comprise: hard capsule, granule, tablet, packed medicinal tea, pulvis.
The present invention also provides the preparation method of described health-care food composition, comprising: get holothurian freeze-dried powder, cervus elaphus linnaeus powder and Fructus lycii P.E, sieve, add pharmaceutically acceptable auxiliary material, technique is prepared into required oral formulations routinely.
Preferably, wherein said tablet is like this preparation: get holothurian freeze-dried powder, cervus elaphus linnaeus powder and Fructus lycii P.E and cross respectively 80 mesh sieves, add filler, disintegrant, mix, add wetting agent and granulate, drying, whole grain adds lubricant, mixes, compressing tablet, dressing dries, and gets tablet.
Preferably, the preparation method of wherein said health-care food composition specifically may further comprise the steps:
1) sieve: holothurian freeze-dried powder, cervus elaphus linnaeus powder and Fructus lycii P.E, dextrin, PVPP, L-HPC, dolomol, coating agent are crossed respectively 80 mesh sieves;
2) mix, granulate: take by weighing holothurian freeze-dried powder, cervus elaphus linnaeus powder and the Fructus lycii P.E of described amount, the dextrin of 70～100 weight portions, the PVPP of 85～115 weight portions, the common mixing 30 minutes of L-HPC of 72～88 weight portions, add 40 ~ 60% edible alcohols, stirred 8 ~ 15 minutes, softwood processed, softwood is crossed 14 ~ 18 mesh sieves and is granulated, 50 ~ 60 ℃ of dryings are after the whole grain of 14 ~ 18 mesh sieves;
3) compressing tablet, dressing: the dolomol that particle adds 5～8 weight portions mixes that compressing tablet gets plain sheet after 2 ~ 6 minutes; It is 8 ~ 10% coating solution that the coating agent of getting 20～25 weight portions is prepared into concentration with 60 ~ 75% edible alcohol, with plain coating tablets, dries, and gets coating tablet.
The present invention further provides the application of described health-care food composition in the product of preparation enhancing immunity.
Holothurian freeze-dried powder described in the present invention, it is sweet, salty to distinguish the flavor of, kidney tonifying, benefit marrow, its benefit warm in nature, pharmacological research show that sea cucumber has effect antitumor, that improve immunity, and wherein rich in protein, arginine etc. are the necessary materials of immune function of human body, can prevent disease infect, adjust the immunity of body.
Cervus elaphus linnaeus powder invigorating kidney yang described in the present invention, benefiting essence-blood, strengthening the bones and muscles contain the Multiple components such as protein, peptide, and pharmacological research shows that it can strengthen cell and humoral immunity ability, suppress the immunologic hypofunction that first ammonia purine causes.
Fructus lycii P.E described in the present invention is nourishing liver and kidney, be used for soreness of waist and knee joint, consumptive disease essence thanks to, contain the compositions such as polysaccharide, pharmacological research shows antalzyme activity in phagocytic function that it can promote Turnover of Mouse Peritoneal Macrophages and the serum, significantly strengthen peritoneal macrophage and be subjected to vitality of subject, strengthen the T cytoactive.
The present invention adopts holothurian freeze-dried powder, cervus elaphus linnaeus powder and Fructus lycii P.E to make the health food that strengthens immunity, and three kinds of raw material reasonable compatibilities can effectively strengthen immunity; Finished product preparation technology is simple, has reduced the cost of manufacture of health food of the present invention; Health food of the present invention is by strengthening immunity function experiment and safety evaluatio experiment as can be known, and it is remarkable that health food of the present invention strengthens immunity function, and safe without toxic side effect, can long-term taking.
The specific embodiment
The invention will be further described below in conjunction with specific embodiment, can be implemented so that those skilled in the art can better understand the present invention also, but illustrated embodiment is not as a limitation of the invention.
In this manual, unless specialize, used technical term is those skilled in the art's Essential Terms; The experimental technique of unreceipted actual conditions is experimental technique routinely in this specification; Used test material is commercially available purchase product if no special instructions in this specification; Wherein used holothurian freeze-dried powder is tieed up special biological Co., Ltd available from the Yantai City, and the cervus elaphus linnaeus powder is available from the emerging ginseng and pilose antler of Xifeng County clock Co., Ltd, and Fructus lycii P.E is available from prosperous Bioisystech Co., Ltd in the Shaanxi.
Embodiment 1
Present embodiment strengthens the health-care food composition of immunity, contains the bulk drug of following weight portion:
Holothurian freeze-dried powder 167; Cervus elaphus linnaeus powder 100; Fructus lycii P.E 158;
The preparation method is as follows:
1) sieves: get holothurian freeze-dried powder, cervus elaphus linnaeus powder, Fructus lycii P.E, dextrin, PVPP, L-HPC, dolomol, coating agent and cross respectively 80 mesh sieves;
2) mix, granulate: by formula rate take by weighing holothurian freeze-dried powder, cervus elaphus linnaeus powder, Fructus lycii P.E and 88 weight portion dextrin, 100 weight portion PVPPs, 82 weight portion L-HPCs are common mixed 30 minutes, add 50% edible alcohol, stirred 15 minutes, softwood processed, softwood is crossed 16 mesh sieves and is granulated, 50 ℃ of dryings are after the whole grain of 16 mesh sieves;
3) compressing tablet, dressing: the 5 weight portion dolomols that added 80 mesh sieves mix that compressing tablet gets plain sheet after 5 minutes; The edible alcohol of getting 23 weight portion coating agent volumetric concentrations and be 60 % is prepared into the coating solution that concentration is 8 %, with plain coating tablets, dries, and gets coating tablet.Every 0.7g of element sheet, dressing weightening finish 3.3%.Every 100g finished product contains thick polysaccharide 1.2g.
Using method: oral, every day 2 times, each 2.1g, one after each meal.
Suitable crowd: immunocompromised person.
Embodiment 2
Present embodiment strengthens the health-care food composition of immunity, contains the bulk drug of following weight portion:
Holothurian freeze-dried powder 145; Cervus elaphus linnaeus powder 130; Fructus lycii P.E 130;
The preparation method is as follows:
1) sieves: get holothurian freeze-dried powder, cervus elaphus linnaeus powder, Fructus lycii P.E, dextrin, PVPP, L-HPC, dolomol, coating agent and cross respectively 80 mesh sieves;
2) mix, granulate: by formula rate take by weighing holothurian freeze-dried powder, cervus elaphus linnaeus powder, Fructus lycii P.E and 100 weight portion dextrin, 115 weight portion PVPPs, 72 weight portion L-HPCs are common mixed 30 minutes, add 50% edible alcohol, stirred 13 minutes, softwood processed, softwood is crossed 16 mesh sieves and is granulated, 50 ℃ of dryings are after the whole grain of 16 mesh sieves;
3) compressing tablet, dressing: the 8 weight portion dolomols that added 80 mesh sieves mix that compressing tablet gets plain sheet after 5 minutes; The edible alcohol of getting 23 weight portion coating agent volumetric concentrations and be 60 % is prepared into the coating solution that concentration is 8 %, with plain coating tablets, dries, and gets coating tablet.Every 0.7g of element sheet, dressing weightening finish 3.3%.Every 100g finished product contains thick polysaccharide 1g.
Using method: oral, every day 2 times, each 2.1g, one after each meal.
Suitable crowd: immunocompromised person.
Embodiment 3
Present embodiment strengthens the health-care food composition of immunity, contains the bulk drug of following weight portion:
Holothurian freeze-dried powder 200; Cervus elaphus linnaeus powder 70; Fructus lycii P.E 180;
The preparation method is as follows:
1) sieves: get holothurian freeze-dried powder, cervus elaphus linnaeus powder, Fructus lycii P.E, dextrin, PVPP, L-HPC, dolomol, coating agent and cross respectively 80 mesh sieves;
2) mix, granulate: by formula rate take by weighing holothurian freeze-dried powder, cervus elaphus linnaeus powder, Fructus lycii P.E and 70 weight portion dextrin, 85 weight portion PVPPs, 88 weight portion L-HPCs are common mixed 30 minutes, add 50% edible alcohol, stirred 13 minutes, softwood processed, softwood is crossed 16 mesh sieves and is granulated, 50 ℃ of dryings are after the whole grain of 16 mesh sieves;
3) compressing tablet, dressing: the 7 weight portion dolomols that added 80 mesh sieves mix that compressing tablet gets plain sheet after 5 minutes; The edible alcohol of getting 20 weight portion coating agent volumetric concentrations and be 60 % is prepared into the coating solution that concentration is 8 %, with plain coating tablets, dries, and gets coating tablet.Every 0.7g of element sheet, dressing weightening finish 2.9%.Every 100g finished product contains thick polysaccharide 1.3g.
Using method: oral, every day 2 times, each 2.1g, one after each meal.
Suitable crowd: immunocompromised person.
Embodiment 4
Present embodiment strengthens the health-care food composition of immunity, contains the bulk drug of following weight portion:
Holothurian freeze-dried powder 155; Cervus elaphus linnaeus powder 110; Fructus lycii P.E 140;
The preparation method is as follows:
1) sieves: get holothurian freeze-dried powder, cervus elaphus linnaeus powder, Fructus lycii P.E, dextrin, PVPP, L-HPC, dolomol, coating agent and cross respectively 80 mesh sieves;
2) mix, granulate: by formula rate take by weighing holothurian freeze-dried powder, cervus elaphus linnaeus powder, Fructus lycii P.E and 100 weight portion dextrin, 115 weight portion PVPPs, 72 weight portion L-HPCs are common mixed 30 minutes, add 50% edible ethanol, stirred 15 minutes, softwood processed, softwood is crossed 16 mesh sieves and is granulated, 55 ℃ of dryings are after the whole grain of 16 mesh sieves;
3) compressing tablet, dressing: the 8 weight portion dolomols that added 80 mesh sieves mix that compressing tablet gets plain sheet after 5 minutes; The edible alcohol of getting 25 weight portion coating agent volumetric concentrations and be 60 % is prepared into the coating solution that concentration is 8 %, with plain coating tablets, dries, and gets coating tablet.Every 0.7g of element sheet, dressing weightening finish 3.6%.Every 100g finished product contains thick polysaccharide 0.9g.
Using method: oral, every day 2 times, each 2.1g, one after each meal.
Suitable crowd: immunocompromised person.
Below study enhancing immunity function and the edible safety (testing according to " health food check and assessment technique standard (2003 editions) " content) of health-care food composition of the present invention.
Acute toxicity test
1. sample: the sample of pressing the preparation of embodiment 1 described prescription and technique.
2. animal used as test: SPF level Kunming mouse.
3. its mouse oral acute toxicity test (MTD): select 20 of 18～22 gram SPF level Kunming mouses, male and female half and half are tested, and with the dosage per os secondary gavage of 15.0g/kgBW, Continuous Observation is 14 days after the administration.Record poisoning manifestations and death condition.
4. result: with the mouse of two kinds of sexes of dosage gavage of 15.0 g/kgBW, observed 14 days.Experimental session has no obvious poisoning manifestations, and nothing is dead in the observation period.Tested material greater than 15.0 g/kgBW, according to " toxicological evaluation of food safety procedure and method " (version in 2003) acute toxicity classification, belongs to nontoxic level to the acute oral toxicity (MTD) of the mouse of two kinds of sexes; The results are shown in Table 1.
The acute toxicity tests of table 1 mouse
30 days feeding trials
1. sample: the sample of pressing the preparation of embodiment 1 described prescription and technique.It is 4.2g/60kgBW day that the people intends with dosage.
2. animal used as test: select body weight 61～82g, 80 of SPF level Wistar rats, male and female half and half.
3. test method: it is 4.2g/60kgBW day that the people intends with dosage, and concrete dosage is designed to: 1.75,3.50,7.00g/kgBW(is equivalent to respectively the people and intends with dosage 25 times, 50 times, 100 times).Rat is divided into three tested material groups and control group at random, 20 every group, male and female half and half.Control group is fed and is raised normal feed, and the tested material group is then fed to raise and mixed the various dose sample.The single cage of animal is fed free diet, Continuous Observation 30 days.
4 observation index and result
4.1 ordinary circumstance is observed:
Observe outward appearance, behavior, toxicity performance and the death condition of animal every day.Weigh weekly, food-intake, calculate weekly food utilization, total foodstuff utilization rate, total food-intake and total augment weight.The food refusal phenomenon does not appear in animal.Each treated animal is food utilization, total foodstuff utilization rate, body weight and weightening finish and control group comparison weekly, the equal not statistically significant of difference (p〉0.05)
4.2 hematological examination:
Measuring hemoglobin content (Hgb), red blood cell (RBC) and leucocyte (WBC) counting, leukocyte differential count (lymph, monokaryon, neutral grain, have a liking for acid, basophilic).Experiment latter stage, hematological indices was all in range of normal value.
4.3 Biochemical Indexes:
Measure serum alanine aminotransferase (ALT), aspartate amino transferase (AST), urea nitrogen (BUN), cholesterol (CHO), triglycerides (TG), blood sugar (GLU), total protein (TP), albumin (ALB), creatinine (CRE).The every biochemical indicator of experiment experimental animal in latter stage is all in range of normal value.
4.4 gross examination of skeletal muscle and pathologic diagnosis:
Animal is put to death in the dislocation of test cervical vertebra in latter stage, observes each main organs and chest, the change of abdominal cavity general pathology.Take out liver, kidney, spleen, the testis of all animals, weigh and calculate organ coefficient.Take out liver, kidney, spleen, testis (or ovary), stomach and the duodenum of control group and high dose group animal, fix with 12% formalin, histological examination is carried out in FFPE, section, HE dyeing under light microscopic.Each main organs (heart, liver, spleen, lung, kidney, stomach, intestines etc.) no abnormality seen.
Strengthen the immunity function experiment
1. sample: the sample of pressing the preparation of embodiment 1 described prescription and technique.The people intended with dosage 4.2g/60kgBW day.
2. experimental animal: 160 of Kunming mouses, entirely female, body weight 18-22g.
3. dosage is selected: tested material establish 700mg/kg.BW, 1400mg/kg.BW, 2100mg/kg.BW low in a Senior Three dosage group (be equivalent to respectively human body recommended intake 10 times, 20 times, 30 times), other establishes the vegetable oil negative control group, every group of 40 animals.Be divided into immune one group, two groups, three groups, four groups, wherein one group is used for Turnover of Mouse Peritoneal Macrophages and engulfs the chicken red blood cell experiment; Two groups are used for NK cytoactive detection and lymphocyte transformation experiment; Three groups are used for antibody-producting cell experiment, serum hemolysin mensuration and the experiment of Delayed onset allergy; Four groups are used for mouse carbon and clean up experiment.Give continuously 30 days, claim weekly body weight one time, adjust the gavage volume.
4. experimental technique
4.1 Turnover of Mouse Peritoneal Macrophages is engulfed chicken red blood cell test (half intracorporal method): gavage is after 30 days continuously, every mouse peritoneal is injected 20% chicken erythrocyte suspension 1ml, after 30 minutes, the cervical vertebra dislocation is put to death, be fixed on the mouse plate, abdominal skin is cut off in the center, through Intraperitoneal injection physiological saline 2 ml, rotated the mouse plate 1 minute, sucking-off abdominal cavity washing lotion 1ml, mean droplet is on 2 slides, placed 30 minutes for 37 ℃, take out in the physiological saline rinsing, dry, fix, 4% Giemsa PBS dyeing 3 minutes, the distilled water rinsing is dried, microscopy.Calculate phagocytic percentage and phagocytic index, the result is as shown in table 2 below.
4.2 NK cytoactive detection (lactate dehydrogenase L DH determination method): gavage is after 30 days continuously, animal is put to death in the cervical vertebra dislocation, take out spleen, tear up, excessively behind 200 eye mesh screens, use Hank, s liquid is washed 3 times, and each centrifugal 10min of 1000r/min gets cytoplasm, the aqua sterilisa splitting erythrocyte is made into 2 * 10 with complete RPMI-1640
7Individual/the ml cell suspension.The cell suspension of each mouse is got 300 μ l be placed in 96 well culture plates, every hole 100 μ l, every hole adds target cell (YAC-1 cell, 4 * 10
5Individual/ml) 100 μ l, do simultaneously each 3 hole of target cell Spontaneous release hole (target cell 100 μ l+ nutrient solutions 100 μ l) and maximum release aperture (target cell 100 μ l+2.5%Triton 100 μ l), 37 ℃ of 5% CO
2Cultivated 4 hours, and took out the centrifugal 5min of 1500r/min.Each hole supernatant 100 μ l is placed another culture plate, and every hole adds 100 μ l matrix liquid again, adds 1mol/L HCL 30 μ l cessation reactions after 10 minutes, measures the OD value at the 490nm place, calculates NK cytoactive rate, and the result is as shown in table 3 below.
4.3 antibody-producting cell detects (Jerne improves slide method): gavage is after 30 days continuously, every mouse peritoneal is injected 2% hematocrit SRBC 0.2ml, the mouse cervical vertebra dislocation of immunity after 5 days half put to death, take out spleen and be placed in the plate that fills Hank ' s liquid, make splenocyte suspension.Agarose is mixed with 1% aqueous solution, 30min is boiled in water-bath, mix with the double concentration Hank ' s of equivalent liquid, the packing small test tube, every pipe 0.5ml, in pipe, add 10%(SA buffer solution preparation again) hematocrit SRBC 50 μ l, each 20 μ l of splenocyte suspension do two Duplicate Samples, rapidly behind the mixing, be poured on the agarose thin layer slide, after agar solidifies, the slide level buckled be placed on the horse, put into CO2gas incubator and hatch 1.5h, then complement is joined in the slide frame groove, continue incubation 1.5h, counting hemolysis plaque number, the result is as shown in table 4 below.
4.4 serum hemolysin is measured (Hemagglutination Method): in continuous gavage after 30 days, every mouse peritoneal injection 2%SRBC 0.2ml immunity, continue gavage after 5 days, extract eyeball and get blood in centrifuge tube, placed 1 hour, peel off, centrifugal 10 minutes of 2000r/min collects serum, with physiological saline serum is made doubling dilution, every part of dilution 12 holes place blood-coagulation-board with the dilution serum of difference, every hole 100 μ l, add again 0.5%SRBC suspension 100 μ l, mixing is placed observed result after 3 hours, is recorded the aggegation degree in every hole for 37 ℃.The calculating antibody product, the result is as shown in table 5 below.
4.5 delayed allergy (DTH) (the sufficient sole of the foot thickens method): gavage is after 30 days, inject the every mouse of 2% hematocrit SRBC(0.2ml/ to mouse peritoneal) sensitization is after 4 days, measure left back sufficient sole of the foot thickness, then at measuring point hypodermic injection 20%(v/v) the every mouse of SRBC(20 μ l/), 24h measures left back sufficient sole of the foot thickness after injection, and same position is measured three times, averages, represent the degree of DTH to attack the sufficient sole of the foot thickness difference in front and back (swelling degree of the paw), the result is as shown in table 6 below.
4.ConA the mouse lymphocyte conversion test (mtt assay) of inducing: at first carry out the preparation of splenocyte suspension.Cell concentration is adjusted into 3 * 10
6Individual/ml, then cell suspension is divided two holes to add in 24 well culture plates, every hole 1ml, a hole adds 75 μ lConA liquid, and another hole compares, and puts 5%CO
237 ℃ of cultivations of incubator 72h.Cultivate and finish front 4h, every hole sucks supernatant 0.7ml gently, adding 0.7ml does not contain the RPMI1640 nutrient solution of calf serum, add simultaneously MTT(5mg/ml) 50 μ l/ holes continuation cultivation 4h, after cultivation finished, it is pure that every hole adds the 1ml acid isopropyl, purple crystal is dissolved fully, then every hole liquid is moved in the cuvette, in 570nm wavelength place's colorimetric estimation OD value, the result is as shown in table 7 below with 723 spectrophotometers.
4.7 mouse carbon clearance test: gavage is after 30 days continuously, in the india ink of mouse tail vein injection with 5 times of normal saline dilutions, immediately timing after pressing 0.1ml/10g prepared Chinese ink and injecting is after injecting prepared Chinese ink the 2nd, 10min gets blood 20 μ l from the angular vein clump respectively, is added to 2ml Na
2CO
3In the solution, with Na
2CO
3Solution is made blank, measures OD value at the 600nm place with 723 spectrophotometers.Get behind the blood mouse is put to death, get liver, spleen is weighed, and calculates phagocytic index, the result is as shown in table 8 below.
Table 2 mouse macrophage engulf the chicken red blood cell ability impact (
± S)
As can be seen from Table 2, gavage is after 30 days continuously, and the phagocytic rate of three dosage treated animals is compared with negative control group with phagocytic index, learns by statistics and processes there was no significant difference (P＞0.05).
The impact of table 3 NK cells in mice activity (
± S)
As can be seen from Table 3, gavage is after 30 days continuously, and three dosage treated animal NK cytoactives are compared with negative control group, learns by statistics and processes difference that there are no significant (P＞0.05).
The impact of table 4 mouse antibodies cellulation (
± S)
As can be seen from Table 4, gavage is after 30 days continuously, and the hemolysis plaque number of three dosage treated animals is compared with negative control group, learns by statistics and processes, and low, high dose group has significant difference (P＜0.05, P＜0.05).
The impact of table 5 mice serum hemolysin (
± S)
As can be seen from Table 5, gavage is after 30 days continuously, and the antibody product of three dosage treated animals is compared with negative control group, learns by statistics and processes, and significant difference (P＜0.01, P＜0.05, P＜0.05) is all arranged.
The impact of table 6 mouse delayed allergy (DTH) (
± S)
As can be seen from Table 6, gavage is after 30 days continuously, and the swelling degree of the paw of three dosage treated animals is compared with negative control group, learns by statistics and processes, and low, high dose group has significant difference (P＜0.05, P＜0.05).
The impact of the mouse lymphocyte conversion test that table 7 ConA induces (
± S)
As can be seen from Table 7, the gavage mouse is after 30 days continuously, and the lymphopoiesis ability of three dosage treated animals is compared with negative control group, learns by statistics and processes there was no significant difference (P＞0.05).
Table 8 mouse monokaryon-macrophage carbon clean up function impact (
± S)
As can be seen from Table 8, gavage is after 30 days continuously, and the carbon of three dosage treated animals is cleaned up ability and compared with negative control group, learns by statistics and processes there was no significant difference (P＞0.05).
In sum, any two aspects result is positive aspect four of cellular immune functions, humoral immune function, monocytes/macrophages function, NK cytoactive, can judge that it has the enhancing immunity function.Conclusion: oral health-care food composition treatment of the present invention increases the advantages such as immunity function is good, safe ready.
The above embodiment is the preferred embodiment that proves absolutely that the present invention lifts, and protection scope of the present invention is not limited to this.Being equal to that those skilled in the art do on basis of the present invention substitutes or conversion, all within protection scope of the present invention.Protection scope of the present invention is as the criterion with claims.
Claims (10)
1. a health-care food composition that strengthens immunity is characterized in that, contains the bulk drug of following weight portion:
Holothurian freeze-dried powder 145～200; Cervus elaphus linnaeus powder 70～130; Fructus lycii P.E 130～180.
2. health-care food composition as claimed in claim 1 is characterized in that, contains the bulk drug of following weight portion:
Holothurian freeze-dried powder 155～180; Cervus elaphus linnaeus powder 90～110; Fructus lycii P.E 140 ~ 170.
3. health-care food composition as claimed in claim 2 is characterized in that, contains the bulk drug of following weight portion:
Holothurian freeze-dried powder 167; Cervus elaphus linnaeus powder 100; Fructus lycii P.E 158.
4. health-care food composition as claimed in claim 1 is characterized in that, the crude protein content in the described holothurian freeze-dried powder by weight percentage 〉=50%.
5. health-care food composition as claimed in claim 1 is characterized in that, the thick polyoses content in the described Fructus lycii P.E by weight percentage 〉=6%.
6. health-care food composition as claimed in claim 1 is characterized in that, described health-care food composition is to make oral formulations; Comprise: hard capsule, granule, tablet, packed medicinal tea, pulvis.
7. the preparation method of each described health-care food composition of claim 1-6, it is characterized in that described preparation method comprises: get holothurian freeze-dried powder, cervus elaphus linnaeus powder and Fructus lycii P.E, sieve, add pharmaceutically acceptable auxiliary material, technique is prepared into required oral formulations routinely.
8. the preparation method of health-care food composition according to claim 7 is characterized in that, described tablet is like this preparation: get holothurian freeze-dried powder, cervus elaphus linnaeus powder and Fructus lycii P.E and cross respectively 80 mesh sieves, add filler, disintegrant, mix, add wetting agent and granulate, dry, whole grain adds lubricant, mixes, compressing tablet, dressing dries, and gets tablet.
9. the preparation method of health-care food composition as claimed in claim 8 is characterized in that, specifically may further comprise the steps:
1) sieve: holothurian freeze-dried powder, cervus elaphus linnaeus powder and Fructus lycii P.E, dextrin, PVPP, L-HPC, dolomol, coating agent are crossed respectively 80 mesh sieves;
2) mix, granulate: take by weighing holothurian freeze-dried powder, cervus elaphus linnaeus powder and the Fructus lycii P.E of described amount, the dextrin of 70～100 weight portions, the PVPP of 85～115 weight portions, the common mixing 30 minutes of L-HPC of 72～88 weight portions, add 40 ~ 60% edible alcohols, stirred 8 ~ 15 minutes, softwood processed, softwood is crossed 14 ~ 18 mesh sieves and is granulated, 50 ~ 60 ℃ of dryings are after the whole grain of 14 ~ 18 mesh sieves;
3) compressing tablet, dressing: the dolomol that particle adds 5～8 weight portions mixes that compressing tablet gets plain sheet after 2 ~ 6 minutes; It is 8 ~ 10% coating solution that the coating agent of getting 20～25 weight portions is prepared into concentration with 60 ~ 75% edible alcohol, with plain coating tablets, dries, and gets coating tablet.
10. the application of each described health-care food composition of claim 1-6 in the product of preparation enhancing immunity.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012103862025A CN102885305A (en) | 2012-10-12 | 2012-10-12 | Health food composition for enhancing immunity and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104397695A (en) * | 2014-11-28 | 2015-03-11 | 山东维尼莱生物科技股份有限公司 | Sea cucumber, lycopene and tea polyphenol tablet and preparation method thereof |
| CN104686768A (en) * | 2013-12-06 | 2015-06-10 | 威海新异生物科技有限公司 | Sea cucumber compressed tablet candy capable of enhancing immunity with high efficiency |
| CN110140962A (en) * | 2019-06-06 | 2019-08-20 | 河北鲜合药业科技有限公司 | A kind of capsule of strengthen immunity and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101991555A (en) * | 2009-08-14 | 2011-03-30 | 上海秀新臣邦医药科技有限公司 | Quetiapine fumarate tablet and preparation method thereof |
| CN102657738A (en) * | 2012-04-20 | 2012-09-12 | 苏州爱斯欧蒂生物科技有限公司 | Health-care product capable of adjusting renal function |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101991555A (en) * | 2009-08-14 | 2011-03-30 | 上海秀新臣邦医药科技有限公司 | Quetiapine fumarate tablet and preparation method thereof |
| CN102657738A (en) * | 2012-04-20 | 2012-09-12 | 苏州爱斯欧蒂生物科技有限公司 | Health-care product capable of adjusting renal function |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104686768A (en) * | 2013-12-06 | 2015-06-10 | 威海新异生物科技有限公司 | Sea cucumber compressed tablet candy capable of enhancing immunity with high efficiency |
| CN104397695A (en) * | 2014-11-28 | 2015-03-11 | 山东维尼莱生物科技股份有限公司 | Sea cucumber, lycopene and tea polyphenol tablet and preparation method thereof |
| CN104397695B (en) * | 2014-11-28 | 2016-05-04 | 山东维尼莱生物科技股份有限公司 | A kind of sea cucumber lycopene tea polyphenol tablet and preparation method thereof |
| CN110140962A (en) * | 2019-06-06 | 2019-08-20 | 河北鲜合药业科技有限公司 | A kind of capsule of strengthen immunity and preparation method thereof |
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Address after: 100062, room 11, 706 Chongwen Avenue, Dongcheng District, Beijing Applicant after: Zhongke Ren (Beijing) Technology Development Co., Ltd. Address before: 100009, room 59, Chai Mo Hutong, 2210, Beijing, Dongcheng District Applicant before: Zhongke Ren (Beijing) Technology Development Co., Ltd. |
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