CN102877299B - Application of superoxide dismutase and trehalose mixture for textile ashing reaction inhibitor to textiles - Google Patents
Application of superoxide dismutase and trehalose mixture for textile ashing reaction inhibitor to textiles Download PDFInfo
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- CN102877299B CN102877299B CN201210403433.2A CN201210403433A CN102877299B CN 102877299 B CN102877299 B CN 102877299B CN 201210403433 A CN201210403433 A CN 201210403433A CN 102877299 B CN102877299 B CN 102877299B
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- superoxide dismutase
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Abstract
The invention relates to a superoxide dismutase and trehalose mixture for a textile ashing reaction inhibitor to textiles. The superoxide dismutase (SOD) and trehalose mixture is based on superoxide dismutase (SOD) and trehalose and is used for removing free radicals in the ashed textiles, the mass concentration of superoxide dismutase (SOD) solution ranges from 0.1mg/mL to 5.0mg/mL, and the mass ratio of the superoxide dismutase (SOD) to the trehalose ranges from 1:2.5 to 1:20. The application aims to provide a simple, convenient and safe ashing reaction inhibition method with good compatibility for the textiles, so that purposes of removing the free radicals and inhibiting ashing reaction in a non-toxic and pollution-free manner are achieved while the original shape of the textiles are unchanged.
Description
Technical field
The present invention relates to a kind of application of the ashing reaction inhibition method for textiles, utilize specifically superoxide dismutase/trehalose to remove the free radical of ashing textiles, thereby suppress the further ashing of textiles in ancient times.
Background technology
Silk goods are one of important innovation and creation of Ancient Times in China, it is the important component part of China's cultural heritage, its enormous amount, kind are numerous, contained message reflection textile technologies and the level of social civilization development in ancient times, witness the development course of Chinese civilization, there is great research value and meaning.Silk fabrics is natural macromolecular material, be to take the polymer that amino acid is unit, preserve and use procedure in, be subject to the impact of external condition (light, heat etc.), easily there is ashing reaction, the free radical of generation special construction.Theoretical according to macromolecule degraded, the existence of free radical can make macromolecular chain generation chain type automatic oxidation reaction, makes its further ashing.Therefore, the existence of free radical is unfavorable to the preservation of silk fabric cultural relics, and the ashing reaction inhibition method of research silk fabrics, has vital meaning for the protection of textiles historical relic.
Superoxide dismutase is a kind of very important oxidoreducing enzyme of defence oxidative damage in organism, and its effect substrate is superoxide anion
, can catalysis superoxide anion
there is disproportionated reaction, thereby remove O
2, play the effect that protection biomolecule is avoided damage.
Trehalose, as a kind of irreducibility disaccharide, has the function of unique protection bioactive materials.Trehalose has protective effect to the enzyme of solution state, and it has larger aquation volume, can compete the water in more protein hydrated sheath, therefore protein structure is piled up more tight, is not vulnerable to the impact of extraneous adverse environment.
Summary of the invention
The object of the present invention is to provide a kind of ashing reaction inhibition method for textiles that compatibility is good, easy, safe, to reaching radicals scavenging, when suppressing ashing reaction, do not change the object of historical relic original state and nontoxic pollution-free.
A kind of method suppressing for textiles ashing reaction, its be superoxide dismutase (SOD)/trehalose built agent as the application of textiles ashing reaction inhibitor, based on superoxide dismutase (SOD)/trehalose for the radicals scavenging effect of ashing textiles.
Be applied in the ashing reaction inhibition method of textiles, it is characterized in that preparing superoxide dismutase (SOD)/trehalose built agent, the mass concentration of superoxide dismutase (SOD) solution is 0.1mg/mL-5.0mg/mL, and superoxide dismutase (SOD) is 1:2.5-1:20 with the mass ratio of trehalose.The ashing reaction inhibition method that is applied in textiles, is characterized in that, with 1cm
2it is standard that ashing textiles sprays 50 μ L-400 μ L SOD/ trehalose built agents, and inhibitor is sprayed to ashing textile surfaces, utilizes film to be sealed, and standing 24h-72h under room temperature, at room temperature makes its air dry afterwards.
Be applied in the ashing reaction inhibition method of textiles, it is characterized in that described silk goods at room temperature place 24-72h.
Use ashing reaction inhibitor, proton can be provided, eliminate the free radical on chain, interrupt the chain growth of autoxidation to stop the degraded of silk fabrics, suppress the ashing reaction of silk fabrics.Therefore ashing reaction inhibitor plays stabilization to the free radical on polymer, stop the further automatic oxidation reaction of macromolecular chain, thereby suppress the further aging of polymer.
beneficial effect of the present invention
EPR is a kind of Wave Spectrum technology that direct-detection and research contain unpaired electron paramagnet, according to the kind of the large I research freedom base of the magnetic field intensity of peak center in epr signal and the g factor.EPR spectrometer record be a differential spectral line of absorption signal, its meter is carried out to the content that area after integration is free radical.Because the unstability of base line of spectral line is fixed, with area, calculate and can produce larger error.The peak height of Free Radical Signal is larger, and the content of free radical is just higher.Therefore, this research adopt the peak height of EPR spectral line represent free-radical contents number.
By three kinds of Ancient Silk Textile (Yangzhou be unearthed silk, Jing Zhou be unearthed silk goods, the six peaces silk goods that are unearthed) with modern silk under the same conditions, carrying out electron paramagnetic resonance (EPR) analyzes, it the results are shown in Figure the epr signal that BL in 1, Fig. 1, JZ, LA, YZ represent respectively modern silk, the unearthed silk goods in Jing Zhou, the unearthed silk goods of six peaces, the unearthed silk in Yangzhou.By research, find, in three Ancient Silk Textile measuring, all contain a kind of relatively stable free radical, the epr signal of this free radical (as shown in Figure 1) does not have hyperfine splitting, for symmetric unimodal, the magnetic field intensity of peak center is all 324.5mT, with manganese standard sample, it is demarcated and is known, its g factor is 2.0037.Peak shape is similar to Bruker standard coal sample.According to the g factor of the structure of fibroin and epr signal thereof and this free radical of position estimating, be to be typically similar to the Ionization Potential of C-Centered Radicals in coal
, simulation heat ageing also has this free radical.The free radical of ashing textiles is different from oxygen radical.This experiment is applied to the superoxide dismutase of biological aspect on ashing textiles first, ashing reaction inhibitor by research preparation for ashing textiles---SOD/ trehalose built agent, inquires into the removing effect of built agent to the Ionization Potential of C-Centered Radicals of textiles.
(1)by modern silk heat ageing 40h at 230 ℃, silk becomes dark-brown, intensity extreme difference, and one touches brokenly, usings this as ashing textiles analog sample.Analog sample is carried out to cutting by 1cm * 1cm size.Compound concentration is the aqueous trehalose of 10.0mg/mL and the superoxide dismutase of 1.0mg/mL (SOD) solution, two kinds of solution equal proportions are mixed, and being mixed with total concentration is 0.55% superoxide dismutase (SOD)/trehalose built agent (superoxide dismutase (SOD) is 1:10 with trehalose mass ratio).Get 2 analog samples that cut and be placed in respectively two culture dishes, according to 1cm
2textile surface sprays the standard of 200 μ L built agents, and built agent is evenly sprayed on to textile surfaces, and the particle diameter of ejection steam is less better, and the impulsive force producing while avoiding the globule to drip the ashing textile surfaces in intensity extreme difference causes damage to it.Use afterwards film to seal (film does not contact with textiles) to culture dish, guarantee that textiles is in moisture state, room temperature is placed 24h, 48h.After being taken out, sample in natural drying at room temperature, does electron paramagnetic resonance (EPR) analysis that blank sample and inhibitor are processed rear sample.In this experiment, utilize the peak height of EPR spectral line to represent the amount of free radical in sample, calculate its free radical scavenging activity.
In Fig. 2, curve 1,2,3 represents that respectively blank analog sample, analog sample are used superoxide dismutase (SOD)/trehalose reaction 24h, analog sample to use the epr signal of superoxide dismutase (SOD)/trehalose reaction 48h, according to the peak height of spectral line calculate superoxide dismutase (SOD)/trehalose built agent in the differential responses time free radical scavenging activity to simulation ashing sample, the results are shown in Table 1.As can be seen from Table 1, superoxide dismutase (SOD)/trehalose built agent has higher removing effect to the free radical of ashing textiles analog sample.The existence of free radical can be accelerated the carrying out of the ashing reaction of textiles, by removing the Ionization Potential of C-Centered Radicals on ashing textiles, to playing of its ashing reaction certain inhibitory action.
Comparison diagram 3-1 and Fig. 3-2, display simulation ashing sample is being used superoxide dismutase (SOD) front and back, and its outward appearance does not change, and illustrates that relatively superoxide dismutase (SOD) can not impact the outward appearance of sample.
Sample strength extreme difference because of 230 ℃ of heat ageing 40h, one touch broken, therefore adopt the sample of 230 ℃ of heat ageing 2h, sample is sprayed to 0.55% superoxide dismutase (SOD)/trehalose built agent, and the aged samples that aged samples and superoxide dismutase (SOD)/trehalose is processed is placed in 60 ℃, the climatic chamber of 80%RH aging 3 days.By sample be cut into size radially * broadwise is 1cm * 5cm, detects the tensile strength of each sample with dynamic thermomechanical analysis apparatus (DMA), result is as following table 2:
By DMA, analyzed and drawn, aged samples is being used ashing reaction inhibitor front and back, and its tensile strength is constant, illustrates that superoxide dismutase (SOD)/trehalose built agent is on the not impact of the intensity of ashing textiles.
By above analysis, show that superoxide dismutase (SOD)/trehalose built agent can not affect the outward appearance of ashing textiles, and to it, be safely and effectively, the clearance rate of free radical can reach more than 65%, has suppressed to a certain extent further carrying out of textiles ashing reaction.
(2)be unearthed in Yangzhou to silk as research object, preparation superoxide dismutase (SOD)/trehalose built agent.Adopt spraying process that ashing reaction inhibitor is evenly sprayed to sample surfaces, 1cm
2textile surface sprays 200 μ L, then at sample surfaces, uses diaphragm seal, and room temperature is placed 24h, 48h, and after sample is taken out, in natural drying at room temperature, the EPR that does blank sample and the rear sample of inhibitor processing analyzes, and calculates its free radical scavenging activity.
In Fig. 4, curve 4,5,6 represents that respectively Yangzhou sample, Yangzhou sample are used superoxide dismutase (SOD)/trehalose reaction 24h, Yangzhou sample to use the epr signal of superoxide dismutase (SOD)/trehalose reaction 48h, according to the peak height of spectral line calculate superoxide dismutase (SOD)/trehalose built agent in the differential responses time free radical scavenging activity to Yangzhou sample.
(3)be unearthed in Jing Zhou to silk goods as research object, through EPR, analyze, the epr signal of the unearthed silk goods in Jing Zhou is very weak, illustrates that its free radical is more stable.By Jing Zhou sample heat ageing 6h at 230 ℃, accelerate its aging its generation free radical that makes.Adopt spraying process that ashing reaction inhibitor is evenly sprayed to sample surfaces, 1cm
2textile surface sprays 200 μ L, then at sample surfaces, uses diaphragm seal, and room temperature is placed 24h, 48h, and after sample is taken out, in natural drying at room temperature, the EPR that does blank sample and the rear sample of inhibitor processing analyzes, and calculates its free radical scavenging activity.
In Fig. 5, curve 7, 8, 9, 10, 11 represent respectively Jing Zhou aged samples, Jing Zhou aged samples is used 0.55% superoxide dismutase (SOD)/trehalose reaction 24h, Yangzhou sample is used 0.55% superoxide dismutase (SOD)/trehalose reaction 48h, Jing Zhou aged samples is used 1.10% superoxide dismutase (SOD)/trehalose reaction 24h, Jing Zhou aged samples is used the epr signal of 1.10% superoxide dismutase (SOD)/trehalose reaction 48h, according to the peak height of spectral line calculate superoxide dismutase (SOD)/trehalose built agent in the differential responses time free radical scavenging activity to Jing Zhou aged samples.
(4)six peaces are unearthed to silk goods as research object, through EPR, analyze, the EPR spectrum peak of the unearthed silk goods of six peaces is very weak, illustrates that its free radical is more stable.By six peace samples heat ageing 6h at 230 ℃, accelerate its aging its generation free radical that makes.Adopt spraying process that ashing reaction inhibitor is evenly sprayed to sample surfaces, 1cm
2textile surface sprays 200 μ L, then at sample surfaces, uses diaphragm seal, and room temperature is placed 24h, 48h, and after sample is taken out, in natural drying at room temperature, the EPR that does blank sample and the rear sample of inhibitor processing analyzes, and calculates its free radical scavenging activity.
In Fig. 6, curve 12, 13, 14, 15, 16 represent respectively six peace aged samples, six peace aged samples are used 0.55% superoxide dismutase (SOD)/trehalose reaction 24h, six peace aged samples are used 0.55% superoxide dismutase (SOD)/trehalose reaction 48h, six peace aged samples are used 1.10% superoxide dismutase (SOD)/trehalose reaction 24h, six peace aged samples are used the epr signal of 1.10% superoxide dismutase (SOD)/trehalose reaction 48h, according to the peak height of spectral line calculate superoxide dismutase (SOD)/trehalose built agent in the differential responses time free radical scavenging activity to six peace aged samples.
Accompanying drawing explanation:
Fig. 1 is the epr signal of the unearthed silk goods in Jing Zhou, the unearthed silk goods of six peaces, the unearthed silk in Yangzhou and blank sample;
Fig. 2 is the epr signal of simulation ashing sample;
Fig. 3-1st, analog sample adds the front photo of superoxide dismutase (SOD);
Fig. 3-2nd, analog sample adds the rear photo of superoxide dismutase (SOD);
Fig. 4 is the epr signal of the unearthed silk in Yangzhou;
Fig. 5 is the epr signal of Jing Zhou aged samples;
Fig. 6 is the epr signal of six peace aged samples;
Fig. 7 is that unearthed textiles aged samples is processed the epr signal of 24h through superoxide dismutase (SOD)/trehalose built agent of 0.55%;
Fig. 8 is that unearthed textiles aged samples is processed the epr signal of 72h through superoxide dismutase (SOD)/trehalose built agent of 2.05%;
Fig. 9 is that unearthed textiles aged samples is processed the epr signal of 72h through superoxide dismutase (SOD)/trehalose built agent of 0.70%.
Specific embodiment
Textiles preserve and use procedure in easily produce free radical, it is research object that modern heat ageing simulation ashing sample and ashing in ancient times textiles are take in the present invention, utilizes ashing reaction inhibitor to carry out radicals scavenging to it, to reach the effect of inhibition ashing.
Embodiment 1
230 ℃ of aging 6h of textiles will be unearthed, superoxide dismutase (the SOD)/trehalose built agent (mass ratio 1:10) of preparation gross mass mark 0.55%, be sprayed on sample, use film to cover sealing, make under its room temperature after standing 24h, dry under natural conditions, its free radical scavenging activity is 30.5%, its EPR the results are shown in Figure 7(curve 17 for the epr signal of the aging 6h of textiles sample that is unearthed, the epr signal of sample after the superoxide dismutase (SOD) that curve 18 is 0.55%/trehalose built agent processing 24h).
Embodiment 2
230 ℃ of aging 6h of textiles will be unearthed, superoxide dismutase (the SOD)/trehalose built agent (mass ratio 1:20) of preparation gross mass mark 2.05%, be sprayed on sample, use film to cover sealing, make under its room temperature after standing 72h, dry under natural conditions, its free radical scavenging activity is 74.5%, its EPR the results are shown in Figure 8(curve 19 for the epr signal of the aging 6h of textiles sample that is unearthed, the epr signal of sample after the superoxide dismutase (SOD) that curve 20 is 2.05%/trehalose built agent processing 72h).
Embodiment 3
230 ℃ of aging 6h of textiles will be unearthed, superoxide dismutase (the SOD)/trehalose built agent (mass ratio 1:2.5) of preparation gross mass mark 0.70%, be sprayed on sample, use film to cover sealing, make under its room temperature after standing 72h, dry under natural conditions, its free radical scavenging activity is 31.8%, its EPR the results are shown in Figure 9(curve 21 for the epr signal of the aging 6h of textiles sample that is unearthed, the epr signal of sample after the superoxide dismutase (SOD) that curve 22 is 0.70%/trehalose built agent processing 72h).
Claims (4)
1. the ashing reaction inhibitor of a textiles, it is characterized in that it is superoxide dismutase (SOD)/trehalose built agent, the mass concentration of superoxide dismutase (SOD) solution is 0.1mg/mL-5.0mg/mL, and superoxide dismutase in built agent (SOD) is 1:2.5-1:20 with the mass ratio of trehalose.
2. the application that a superoxide dismutase (SOD)/trehalose built agent suppresses for textiles ashing reaction, it is that superoxide dismutase (SOD) solution and aqueous trehalose are composite with certain proportion, compound concentration is the aqueous trehalose of 10.0mg/mL and the superoxide dismutase of 1.0mg/mL (SOD) solution, two kinds of solution equal proportions are mixed, being mixed with total concentration is 0.55% superoxide dismutase (SOD)/trehalose built agent, superoxide dismutase in built agent (SOD) is 1:10 with trehalose mass ratio, radicals scavenging based on superoxide dismutase (SOD)/trehalose for ashing textiles.
3. superoxide dismutase (SOD)/trehalose built agent is applied on textiles as the application process of ashing reaction inhibitor according to claim 2, it is characterized in that, with 1cm
2it is standard that ashing textiles sprays 50 μ L-400 μ L superoxide dismutase (SOD)/trehalose built agents, inhibitor is sprayed to ashing textile surfaces, utilize film to be sealed, standing 24h-72h under room temperature, at room temperature makes its air dry afterwards.
4. superoxide dismutase (SOD)/trehalose built agent is applied on textiles as the application process of ashing reaction inhibitor according to claim 3, it is characterized in that described textiles at room temperature places 24-36h.
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| CN1113516A (en) * | 1994-06-14 | 1995-12-20 | 北京市联合大学 | Bioenzyme used for dismounting pictures and the method therefor |
| CN1245831A (en) * | 1999-09-10 | 2000-03-01 | 袁勤生 | High temp. non-inactivation preparation method of superoxide dismutase |
| CN101492882A (en) * | 2008-12-31 | 2009-07-29 | 荆州文物保护中心 | Use method of bacteria cellulose in ancient protection of wrought silk cultural relics |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1113516A (en) * | 1994-06-14 | 1995-12-20 | 北京市联合大学 | Bioenzyme used for dismounting pictures and the method therefor |
| CN1245831A (en) * | 1999-09-10 | 2000-03-01 | 袁勤生 | High temp. non-inactivation preparation method of superoxide dismutase |
| CN101492882A (en) * | 2008-12-31 | 2009-07-29 | 荆州文物保护中心 | Use method of bacteria cellulose in ancient protection of wrought silk cultural relics |
| CN101613937A (en) * | 2009-08-10 | 2009-12-30 | 浙江理工大学 | The bionical method for strengthening of silk-fibroin compound system of silk historical relic |
Non-Patent Citations (4)
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