CN102876655A - Immobilization method of microorganism for repairing petroleum-contaminated soil - Google Patents
Immobilization method of microorganism for repairing petroleum-contaminated soil Download PDFInfo
- Publication number
- CN102876655A CN102876655A CN2012103313653A CN201210331365A CN102876655A CN 102876655 A CN102876655 A CN 102876655A CN 2012103313653 A CN2012103313653 A CN 2012103313653A CN 201210331365 A CN201210331365 A CN 201210331365A CN 102876655 A CN102876655 A CN 102876655A
- Authority
- CN
- China
- Prior art keywords
- bacterium
- immobilization
- crude oil
- carrier
- microorganism
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 27
- 244000005700 microbiome Species 0.000 title claims abstract description 24
- 239000002689 soil Substances 0.000 title claims abstract description 22
- 241000894006 Bacteria Species 0.000 claims abstract description 45
- 239000000463 material Substances 0.000 claims abstract description 21
- 239000010779 crude oil Substances 0.000 claims abstract description 20
- 238000006731 degradation reaction Methods 0.000 claims abstract description 19
- 230000015556 catabolic process Effects 0.000 claims abstract description 18
- 238000001179 sorption measurement Methods 0.000 claims abstract description 10
- 230000001580 bacterial effect Effects 0.000 claims abstract description 3
- 239000007788 liquid Substances 0.000 claims description 16
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 239000001888 Peptone Substances 0.000 claims description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 7
- 239000003921 oil Substances 0.000 claims description 7
- 230000001954 sterilising effect Effects 0.000 claims description 7
- 238000004659 sterilization and disinfection Methods 0.000 claims description 7
- 235000015278 beef Nutrition 0.000 claims description 6
- 235000015097 nutrients Nutrition 0.000 claims description 6
- 239000012153 distilled water Substances 0.000 claims description 5
- 238000012360 testing method Methods 0.000 claims description 5
- 230000005526 G1 to G0 transition Effects 0.000 claims description 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 4
- 239000011780 sodium chloride Substances 0.000 claims description 4
- 239000002253 acid Substances 0.000 claims description 3
- 150000001875 compounds Chemical class 0.000 claims description 3
- 150000003839 salts Chemical class 0.000 claims description 3
- 238000005303 weighing Methods 0.000 claims description 3
- 108010080698 Peptones Proteins 0.000 claims description 2
- 230000004913 activation Effects 0.000 claims description 2
- 240000001791 bacterium SM3 Species 0.000 claims description 2
- 238000006243 chemical reaction Methods 0.000 claims description 2
- 238000012856 packing Methods 0.000 claims description 2
- 235000019319 peptone Nutrition 0.000 claims description 2
- 238000010298 pulverizing process Methods 0.000 claims description 2
- 239000009671 shengli Substances 0.000 claims description 2
- 239000000758 substrate Substances 0.000 claims description 2
- 238000004064 recycling Methods 0.000 abstract description 3
- 238000011084 recovery Methods 0.000 abstract description 2
- 239000010902 straw Substances 0.000 abstract 3
- 239000000969 carrier Substances 0.000 abstract 2
- 239000002699 waste material Substances 0.000 abstract 1
- 238000005516 engineering process Methods 0.000 description 14
- 239000012530 fluid Substances 0.000 description 6
- 239000003208 petroleum Substances 0.000 description 6
- 230000000593 degrading effect Effects 0.000 description 5
- 230000000813 microbial effect Effects 0.000 description 5
- 230000007812 deficiency Effects 0.000 description 4
- 239000002245 particle Substances 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 238000005067 remediation Methods 0.000 description 4
- 238000010521 absorption reaction Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000010855 food raising agent Nutrition 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 229930000044 secondary metabolite Natural products 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Landscapes
- Processing Of Solid Wastes (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention discloses an immobilization method of microorganisms for repairing petroleum-polluted soil, which is characterized in that straws are selected as carriers, the microorganisms are adsorbed on straw material carriers for immobilization under the optimal adsorption conditions that the immobilization time is 15 hours, the immobilization temperature is 30 ℃, the rotating speed is 160r/min and the carrier quantity is bacterial quantity =1:4-7, the straw materials fixed with the microorganisms are directly thrown into the petroleum-polluted soil, secondary pollution is not caused, recovery is not needed, the problem of recycling is not involved, and the degradation rate of crude oil is improved by nearly 40 percent compared with that of free bacteria. The invention provides an effective way for resource utilization of waste crops.
Description
Technical field
The present invention relates to a kind of immobilization method of microorganism for remedying oil-polluted soils, the method belongs to microbial immobilized technology, specifically the process for fixation of a kind of immobilized microorganism for remedying oil-polluted soils.
Background technology
Oil-polluted soils is one of important pollution of oil and petroleum chemical enterprise, and petroleum pollution wherein has very high toxic action to human body and environment.The treatment process of oil-polluted soils mainly contains both at home and abroad: burning method, landfill method, stacking method and biological restoration etc.
The core of microorganism recovery technique is that efficient degrading bacteria is to the Degradation of petroleum pollution, it is more complicated that degradation process trends towards the composition of pollutent, the toxicity of some secondary metabolites and middle degraded product is larger, the deficiency of free microorganism repair just displays gradually, as in the unit volume effectively degradation bacteria concentration low, be in weak tendency, toxin immunity infringement ability etc. with indigenous bacterium competition.And microbial immobilized technology can overcome these shortcomings and drawback, and repairing effect is greatly improved.
Microbial immobilized technology is a kind of new bio technology of rising the eighties in 20th century, refer to will be free cell (microorganism) or the enzyme area of space that is positioned to limit in, and make it keep technology active and can Reusability.Although microbial immobilized technology is widely used in repairing in contaminated fluid medium (such as waste water) and semi-fluid medium (such as the mud) environment, but be applied to the research of restoring petroleum-polluted non-fluid medium (such as soil), report less both at home and abroad.
The major cause that the restriction immobilized microorganism is used in soil remediation is complicacy and the singularity that soil non-fluid medium itself has, proposed many problem in science that face when being different from fluid medium applying immobilized technology for this technology, exist deficiency such as immobilization technology itself, such as the untapped immobilization technology that goes out to be applicable to non-fluid medium, the untapped immobilization technology that goes out broad spectrum, not yet solve the immobilization problem of mixed bacterium, and lack the environmental safety evaluation of immobilization particle and the systematic study that environmental factor in the immobilization process is regulated and control; The carrier of immobilization microorganism particles is selected, and how to overcome the impact that the nonuniformity of the not returnability of immobilization particle in soil and Pollutant levels causes immobilization particle; How to guarantee the bacteria concentration of the immobilized microorganism of absorption method preparation.
Summary of the invention
Technical problem to be solved by this invention is to propose a kind of immobilization method of microorganism for remedying oil-polluted soils, to solve the dense deficiency of expensive poor efficiency, bacterium, these deficiencies of introducing secondary pollution in the present soil remediation process; And natural plant material can be used as the raising agent of soil, for the microorganism rehabilitating soil provides nutritive substance, for the recycling of rejected material provides an effective approach.
The present invention solves the technical scheme that its technical problem takes: do carrier with stalk material, under the condition of optimal adsorption time, temperature, carrier amount, microorganism adsorption is carried out immobilization at stalk vector, specifically realize according to the following steps:
1. the screening high efficient petroleum degrading bacteria screens oil degradation bacteria from petroleum-polluted soil, obtain a strain degradation rate and be single bacterium SM-3 of 23%, this bacterium is rod bacterium, measures the growth curve of this bacterium, the logarithmic phase of this bacterial strain is 5-16h, and be 16-25h stationary phase;
2. take Shengli crude as reaction substrate, measure the typical curve of this crude oil, in order to calculate degradation rate;
3. selecting stalk material is fixation support, with crushed stalk, crosses 80 mesh sieves.
4. get the stalk material 3%-5%(V/V after the pulverizing) in the reactor of the certain volume of packing into after the compacting, with 121 ℃ of high-pressure water vapors sterilization 20min, will be 1 * 10 with the density behind the beef extract-peptone liquid nutrient medium activation 16h then
10-5 * 10
10The bacterium liquid SM-3 of individual/mL is by 20%(V/V) receive in the reactor, fix 12～24h in 30～40 ℃ of constant temperature;
The used bacterium of immobilization is SM-3, and this bacterium is cultured to logarithmic phase and point of interface stationary phase (16h) with the beef extract-peptone liquid nutrient medium, and the degradation rate to crude oil under its unbound state is 23%;
The prescription of described beef extract-peptone liquid nutrient medium is: NaCl 1%(m/m), peptone 1%(m/m), extractum carnis 0.5%(m/m) and the distilled water of certain volume mix, take the pH test paper as indication, regulate pH7.0 with NaOH or hydrochloric acid, 121 ℃ of water vapour sterilization 20min.
5. having fixed the 3%-5%(V/V of bacterium) the related loose bacterium liquid of carrier transfers in the Centrifuge Cup together, centrifugal 10min under 1000r/min, outwell upper strata bacterium liquid, lower floor's carrier aseptic technique is transferred in the previously prepd crude oil substratum fully, 30 ℃ of constant temperature degraded 7d, degradation rate can reach 60%, and the specific ionization bacterium improves nearly 40%;
The compound method of described crude oil substratum is first to take by weighing 0.4%(m/m in the reactor of certain volume) about crude oil, the record exact mass, measuring again 20%(V/V) basic minimal medium is to the reactor that weighs up crude oil, and the background oil concentration is about 10000mg/L; The prescription of basis minimal medium is NaCl 0.5%(m/m), MgSO
47H
2O 0.025%(m/m), (NH
4)
2SO
40.1%(m/m), NaNO
30.2%(m/m), K
2HPO
43H
2O 1%(m/m), KH
2PO
40.4%(m/m) and the distilled water of certain volume mix, take the pH test paper as indication, with NaOH or salt acid for adjusting pH to 7-7.5,121 ℃ of water vapour sterilization 20min.
6. the leaf before the immobilization is carried out BET and characterize, the leaf before and after degrading before the immobilization and behind the fixation of microbe is carried out electron-microscope scanning, obtain the internal structure performance of this carrier, estimate its immobilization effect.
The invention has the beneficial effects as follows: because the present invention uses absorption method and prepares immobilized microorganism, directly add the absorption of bacterium liquid in the stalk material carrier of the bacterium of having gone out fixing, therefore, do not need loaded down with trivial details preparation technology, the stalk material that is fixed with microorganism directly is added to by the soil of petroleum pollution, can not introduce secondary pollution, do not need to reclaim, do not relate to the problem of recycling, the free bacterium of the degradation rate of crude oil is improved nearly 40%, reduced cost, improved remediation efficiency.
Embodiment:
Further describe the present invention below by embodiment.
Immobilization method of microorganism of the present invention is to do carrier with stalk material, under the condition of optimal adsorption time, temperature, carrier amount, microorganism adsorption is carried out immobilization at stalk vector, and concrete steps are as follows:
1. the screening high efficient petroleum degrading bacteria is measured growth curve of bacteria.
2. measure the crude oil typical curve, in order to calculate degradation rate.
3. stalk material is carried out composition analysis, record its available phosphorus, total nitrogen and organic content and be respectively 0.508 ‰ (508.38mgkg
-1), 5.58% (wt) and 5.24% (wt).
4. take stalk material as fixation support, investigate respectively adsorption time, temperature, shaking speed and carrier amount to inhaling the impact of bacterium amount with colony counting method, calculate fixed rate, in conjunction with the degradation rate of the leaf immobilized microorganism for preparing under the experiment condition to crude oil, determine in the reactor of certain volume, when adsorption time is 12-24h, temperature is 30-40 ℃, the carrier amount is 3%-5%(V/V), bacterium liquid is 20%(V/V) time, its fixed rate and oil degradation rate are better; Be 15h in the immobilization time, the immobilization temperature is 30 ℃, and the carrier amount is 4%(V/V) time, immobilization effect is best.
5. the leaf immobilized bacterium under the preparation top condition, namely in the reactor of certain volume, carrier amount 4%(V/V), connect bacterium amount 20%, immobilization time 15h, 30 ℃ of immobilization temperature.With the 4%(V/V behind the fixation of microbe) after carrier removed upper strata bacterium liquid, aseptic technique was transferred in the ready crude oil substratum fully, 30 ℃ of constant temperature degraded 7d, degradation rate can reach 60%, and the specific ionization bacterium improves nearly 40%.The compound method of described crude oil substratum is: first take by weighing 0.4%(m/m in the reactor of certain volume) about crude oil, the record exact mass, measuring again 20%(V/V) basic minimal medium is to the reactor that weighs up crude oil, and the background oil concentration is about 10000mg/L; The prescription of basis minimal medium is NaCl0.5%(m/m), MgSO
47H
2O 0.025%(m/m), (NH
4)
2SO
40.1%(m/m), NaNO
30.2%(m/m), K
2HPO
43H
2O 1%(m/m), KH
2PO
40.4%(m/m) and the distilled water of certain volume mix, take the pH test paper as indication, with NaOH or salt acid for adjusting pH to 7-7.5,121 ℃ of water vapour sterilization 20min.
Leaf before the immobilization is carried out BET characterize, recording its specific surface area is 2.0988m
2G
-1, pore volume 0.0030cm
3G
-1, aperture 6.585nm shows that stalk material only has simple meso-hole structure, material is mainly realized fixing bacterium by surface adsorption; Leaf before and after degrading before the immobilization and behind the fixation of microbe is carried out electron-microscope scanning, the result shows that pore structure is mainly cylinder hole, bacterium mainly is adsorbed on the surface of leaf, it is loose that material structure after the degraded can become, bacterium easily comes off, can not reuse, but because it is pollution-free, need not reclaim, can satisfy the application of soil remediation.
The present invention has proposed a kind ofly can guarantee microbial bacteria concentration, the immobilized microorganism preparation method that is used for remedying oil-polluted soils that need not reclaim on the basis of determining suitable solid support material and immobilization technology.
Claims (1)
1. immobilization method of microorganism that is used for remedying oil-polluted soils, the method is to adopt the stalk material carrier, under the condition of control adsorption time, temperature, rotating speed, carrier amount, microorganism adsorption is carried out immobilization at the stalk material carrier, it is characterized in that implementing the specifically suddenly as follows of this method:
1) screen oil degradation bacteria from petroleum-polluted soil, obtain a strain degradation rate and be single bacterium SM-3 of 23%, this bacterium is rod bacterium, measures the growth curve of this bacterium, and the logarithmic phase of this bacterial strain is 5-16h, and be 16-25h stationary phase;
2) take Shengli crude as reaction substrate, measure the typical curve of this crude oil, in order to calculate degradation rate;
3) selecting stalk material is fixation support, and stalk material is pulverized, and crosses 80 mesh sieves;
4) get stalk material after the pulverizing by 3%-5%(V/V) in the reactor of the certain volume of packing into after the compacting, with 121 ℃ of high-pressure water vapors sterilization 20min, will be 1 * 10 with the density behind the beef extract-peptone liquid nutrient medium activation 16h then
10-5 * 10
10The bacterium liquid SM-3 of individual/mL is by 20%(V/V) receive in the reactor, fix 12～24h in 30～40 ℃ of constant temperature;
The used bacterium of immobilization is SM-3, and this bacterium is cultured to logarithmic phase and point of interface stationary phase (16h) with the beef extract-peptone liquid nutrient medium, and the degradation rate to crude oil under its unbound state is 23%;
The prescription of described beef extract-peptone liquid nutrient medium is: NaCl 1%(m/m), peptone 1%(m/m), extractum carnis 0.5%(m/m) and the distilled water of certain volume mix, take the pH test paper as indication, regulate pH7.0 with NaOH or hydrochloric acid, 121 ℃ of water vapour sterilization 20min;
5) the 3%-5%(V/V that has fixed bacterium liquid) the related loose bacterium liquid of carrier transfers in the Centrifuge Cup together, centrifugal 10min under 1000r/min, outwell upper strata bacterium liquid, lower floor's carrier aseptic technique is transferred in the previously prepd crude oil substratum fully, 30 ℃ of constant temperature degraded 7d, degradation rate can reach 60%, and the specific ionization bacterium improves nearly 40%;
The compound method of described crude oil substratum is first to take by weighing 0.4%(m/m in the reactor of certain volume) about crude oil, the record exact mass, measuring again 20%(V/V) basic minimal medium is to the reactor that weighs up crude oil, and the background oil concentration is about 10000mg/L; The prescription of basis minimal medium is NaCl 0.5(m/m), MgSO
47H
2O 0.025%(m/m), (NH
4)
2SO
40.1%(m/m), NaNO
30.2%(m/m), K
2HPO
43H
2O1%(m/m), KH
2PO
40.4%(m/m) and the distilled water of certain volume mix, take the pH test paper as indication, with NaOH or salt acid for adjusting pH to 7-7.5,121 ℃ of water vapour sterilization 20min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012103313653A CN102876655A (en) | 2012-09-07 | 2012-09-07 | Immobilization method of microorganism for repairing petroleum-contaminated soil |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012103313653A CN102876655A (en) | 2012-09-07 | 2012-09-07 | Immobilization method of microorganism for repairing petroleum-contaminated soil |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102876655A true CN102876655A (en) | 2013-01-16 |
Family
ID=47478174
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012103313653A Pending CN102876655A (en) | 2012-09-07 | 2012-09-07 | Immobilization method of microorganism for repairing petroleum-contaminated soil |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102876655A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107828771A (en) * | 2017-11-21 | 2018-03-23 | 山东科技大学 | A kind of preparation method of immobilization oil degradation bacteria and the method based on immobilization oil degradation bacteria processing petroleum pollution |
| CN108718578A (en) * | 2018-04-17 | 2018-11-02 | 仁维国际股份有限公司 | Soil improvement method |
| CN113522960A (en) * | 2020-04-14 | 2021-10-22 | 中国石油天然气股份有限公司 | Method for restoring petroleum hydrocarbon polluted soil |
| CN114505340A (en) * | 2022-02-22 | 2022-05-17 | 中国石油大学(华东) | Method for restoring petroleum-polluted soil by sludge-based biochar immobilized microorganisms |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102085530A (en) * | 2009-12-04 | 2011-06-08 | 中国科学院生态环境研究中心 | Microbial ecological regulation method for repairing petroleum polluted saline-alkali soil |
-
2012
- 2012-09-07 CN CN2012103313653A patent/CN102876655A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102085530A (en) * | 2009-12-04 | 2011-06-08 | 中国科学院生态环境研究中心 | Microbial ecological regulation method for repairing petroleum polluted saline-alkali soil |
Non-Patent Citations (1)
| Title |
|---|
| 张秀霞 等: "微生物固定化载体的选择及其性能", 《化工进展》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107828771A (en) * | 2017-11-21 | 2018-03-23 | 山东科技大学 | A kind of preparation method of immobilization oil degradation bacteria and the method based on immobilization oil degradation bacteria processing petroleum pollution |
| CN108718578A (en) * | 2018-04-17 | 2018-11-02 | 仁维国际股份有限公司 | Soil improvement method |
| CN113522960A (en) * | 2020-04-14 | 2021-10-22 | 中国石油天然气股份有限公司 | Method for restoring petroleum hydrocarbon polluted soil |
| CN114505340A (en) * | 2022-02-22 | 2022-05-17 | 中国石油大学(华东) | Method for restoring petroleum-polluted soil by sludge-based biochar immobilized microorganisms |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109207413B (en) | A kind of highly effective petroleum degradation composite bacteria agent and the preparation method and application thereof | |
| CN106148318B (en) | A kind of preparation and application of charcoal immobilized microbial inoculum | |
| Canakci et al. | Effect of bacterial calcium carbonate precipitation on compressibility and shear strength of organic soil | |
| Zhang et al. | Removal of chlortetracycline from water by Bacillus cereus immobilized on Chinese medicine residues biochar | |
| CN103436464B (en) | Low temperature-resistant petroleum-degrading bacillus sp. strain, culture method and application thereof | |
| CN107376940B (en) | Composite catalyst material for quickly and efficiently repairing organic contaminated soil and preparation method thereof | |
| CN109504398A (en) | A kind of humic acid nano zero valence iron soil Cr pollution amelioration agent and preparation method | |
| CN104946620A (en) | Immobilized microbial inoculant for restoring petroleum-hydrocarbon-polluted alkaline-saline soil and preparation method thereof | |
| CN103484447B (en) | A kind of preparation method of oil degradation zymin and application | |
| CN104560938A (en) | Preparation method and application of petroleum-degrading bacteria immobilized embedded particles | |
| Wang et al. | Contrasting effects of aged and fresh biochars on glucose-induced priming and microbial activities in paddy soil | |
| CN108130299B (en) | Microbial strain for degrading petroleum hydrocarbon and combined repairing agent | |
| CN101724582A (en) | Immobilized microbial inoculum for remediating PAHs contaminated soil and preparation method thereof | |
| CN104368306A (en) | Preparation method of biochar-like matter used for heavy metal contaminated soil repair | |
| CN109821892B (en) | In-situ remediation organic contaminated soil biological material, and preparation method and application thereof | |
| CN114133043B (en) | Preparation method and application of slow-release carbon source material | |
| CN109926448A (en) | A kind of Bacillus foecalis alkaligenes of curing heavy metal and its application | |
| CN102876655A (en) | Immobilization method of microorganism for repairing petroleum-contaminated soil | |
| Qi et al. | Removal efficiency and enzymatic mechanism of dibutyl phthalate (DBP) by constructed wetlands | |
| CN109679871A (en) | A kind of method of PAM-SA immobilized microorganism degradation oily waste water | |
| CN107151663B (en) | Immobilized microbial agent prepared from kelp residue and used for petroleum pollution remediation | |
| Zhao et al. | Application of biochar in the remediation of contaminated soil with high concentration of lead and zinc | |
| Ren et al. | Enhanced biodegradation of oil-contaminated soil oil in shale gas exploitation by biochar immobilization | |
| CN113943580A (en) | Soil remediation material with dual functions of adsorption and degradation, and preparation and application thereof | |
| Sarkar et al. | Strategic utilization of groundnut shell (Arachis hypogaea) immobilized bacterial consortium for enhanced 4-nitrophenol remediation: statistical optimization, kinetic modeling, and reusability |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20130116 |