CN102875631A - Method for extracting tea saponin from tea-seed pancake - Google Patents
Method for extracting tea saponin from tea-seed pancake Download PDFInfo
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- CN102875631A CN102875631A CN2012103915554A CN201210391555A CN102875631A CN 102875631 A CN102875631 A CN 102875631A CN 2012103915554 A CN2012103915554 A CN 2012103915554A CN 201210391555 A CN201210391555 A CN 201210391555A CN 102875631 A CN102875631 A CN 102875631A
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Abstract
The invention discloses a method for extracting tea saponin from a tea-seed pancake. The method comprises the following steps of: crushing tea seed cake meal and drying, and putting into a Soxhlet extractor; adding an isopropanol extracting agent, wherein the weight volume ratio of the tea seed cake meal to the isopropanol extracting agent is 1 to 3; and heating to perform reflux extraction, distilling, evaporating to obtain high-purity tea saponin, wherein the used isopropanol extracting agent can be recycled. The method has the advantages that the extraction rate is high, the extraction speed is high, and the obtained product is high in purity; and the isopropanol is rich in source and low in price, can be recycled and is an ideal extracting agent.
Description
Technical field
The present invention relates to tea seed product deep process technology field, relate in particular to a kind of method of from tea-seed pancake, extracting tea saponin.
Background technology
Tea saponin is a kind of pentacyclic triterpene saponin in the tea seed, and it is the baroque glycoside compound that a class is combined into by part, sugared body and organic acid.Thick tea saponin is generally faint yellow amorphous solid to brown, and property is crisp, has well water-soluble.During its aqueous solution vibration, can produce persistent foam.So it is a kind of good natural detergent and emulsifying agent, can use the aspects such as grease emulsifying, daily-use chemical industry and textile industry.
Oil tea has widely cultivation in mountain area, each province, China south of the River, only Fujian Province's cultivated area just reaches about 3,000,000 mu, have a large amount of grouts by-products an every year in tea-seed oil production, therefore, from tea waste, extract tea saponin, and the research of comprehensive utilization, for the development and use of Fujian Province's oil tea resource, have larger meaning.The content of tea saponin in the tea waste, different slightly variant because of kind, cultivation condition etc., be generally the degreasing cake 13～15%. in addition, still contain starch, protein, oligosaccharides and Mierocrystalline cellulose etc. in the tea waste.
One of method of existing extraction tea saponin is with 80 ℃ of hot water tea waste behind the soak degreasing repeatedly, then filters, concentrates, dry, obtains auburn saponin.Although the method extraction agent is easy to get, technical process is longer, and fuel consumption is also more, and products obtained therefrom purity is lower again, makes its purposes be subject to certain limitation; Two of method is to extract tea saponin with the ethanol equal solvent as extraction agent, use this method, though products obtained therefrom purity is improved, but extraction agent consumption is many, cost is higher, formerly apply for a patent ZL 201110165902.7 " a kind of method of from tea seed, extracting camellia seed oil and tea saponin " although mention in the text and can adopt Virahol to extract tea saponin as extraction agent, but it does not specifically openly adopt Virahol as the technical scheme of extraction agent, the extraction agent that its disclosed method ethanol equal solvent consumes when extracting tea saponin as extraction agent is many, and the purity of gained tea saponin is also low.
Summary of the invention
For overcoming the deficiencies in the prior art, the invention provides the method for from tea-seed pancake, extracting tea saponin that a kind of cost is low, the rear tea saponin product purity of extraction is high.
The present invention reaches the technical scheme that above-mentioned technical purpose adopts to be: a kind of method of from tea-seed pancake, extracting tea saponin, and it may further comprise the steps:
Step 01: with tea waste pulverize dry after, take by weighing the tea waste powder of a weight part, put into apparatus,Soxhlet's, add the isopropanol extraction agent of one to three weight volume ratio, the refluxing extraction of heating 1-2 hour;
Step 02: with isolated all extracting solution distillations in step 01 apparatus,Soxhlet's, reclaim the extraction agent Virahol, the residue after the distillation is thick tea saponin;
Step 03: the thick tea saponin of step 02 gained is moved in the furnace pot, after water-bath is heated to constant weight, obtain refined theasapogenin;
Step 04: with the residue heating in water bath that retains in step 01 apparatus,Soxhlet's, steam the extraction agent Virahol that remains in the residue, the Virahol that step 02 and step 04 reclaim can recycle for step 01.
The water content of Virahol is less than or equal to 0.2% in the described step 01.
In the described step 01 reflux extracting time be 1.5 hours for best.
It is best when tea waste powder and extraction agent weightmeasurement ratio are 3:8 in the described step 01.
Before described step 01, with industrial naptha the Residual oil in the tea waste powder is leached, product purity is further improved.
Before described step 01, the organic acid in the Virahol is removed.
The present invention adopts Virahol to extract tea saponin as extraction agent from tea waste, the present invention has the extraction yield height, extraction rate is fast, products obtained therefrom purity advantages of higher, the Virahol source is than horn of plenty, price is also more cheap, and Virahol can repeat to recycle, and is a kind of comparatively desirable extraction agent.
Embodiment
By describing technology contents of the present invention, structural attitude in detail, realized purpose and effect, give explanation below in conjunction with embodiment is detailed.
Embodiment 1:
Step 01: with tea waste pulverize dry after, take by weighing tea waste powder 75 grams, put into 500 milliliters apparatus,Soxhlet's, add 200 milliliters of the isopropanol extraction agent of moisture content 0.1%, the refluxing extraction of heating 1.5 hours;
Step 02: with isolated all extracting solution distillations in step 01 apparatus,Soxhlet's, reclaim extraction agent Virahol 145ml, the residue after the distillation is thick tea saponin;
Step 03: the thick tea saponin of step 02 gained is moved in the furnace pot, after water-bath is heated to constant weight, obtain refined theasapogenin 9.5 grams, gained tea saponin purity is greater than 97.3%;
Step 04: with the residue heating in water bath that retains in step 01 apparatus,Soxhlet's, steam the extraction agent Virahol that remains in the residue, reclaim Virahol 45ml, the Virahol that step 02 and step 04 reclaim can recycle for step 01.
Embodiment 2:
Step 01: with tea waste pulverize dry after, take by weighing tea waste powder 75 grams, put into 500 milliliters apparatus,Soxhlet's, add 75 milliliters of the isopropanol extraction agent of moisture content 0.2%, the refluxing extraction of heating 2 hours;
Step 02: with isolated all extracting solution distillations in step 01 apparatus,Soxhlet's, reclaim extraction agent Virahol 55ml, the residue after the distillation is thick tea saponin;
Step 03: the thick tea saponin of step 02 gained is moved in the furnace pot, after water-bath is heated to constant weight, obtain refined theasapogenin 4.1 grams, gained tea saponin purity is greater than 85%;
Step 04: with the residue heating in water bath that retains in step 01 apparatus,Soxhlet's, steam the extraction agent Virahol that remains in the residue, reclaim Virahol 10ml, the Virahol that step 02 and step 04 reclaim can recycle for step 01.
Embodiment 3:
Step 01: with tea waste pulverize dry after, take by weighing tea waste powder 75 grams, put into 500 milliliters apparatus,Soxhlet's, add 175 milliliters of the isopropanol extraction agent of moisture content 0.2%, the refluxing extraction of heating 2 hours;
Step 02: with isolated all extracting solution distillations in step 01 apparatus,Soxhlet's, reclaim extraction agent Virahol 140ml, the residue after the distillation is thick tea saponin;
Step 03: the thick tea saponin of step 02 gained is moved in the furnace pot, after water-bath is heated to constant weight, obtain refined theasapogenin 8.8 grams, gained tea saponin purity is greater than 95%;
Step 04: with the residue heating in water bath that retains in step 01 apparatus,Soxhlet's, steam the extraction agent Virahol that remains in the residue, reclaim Virahol 16ml, the Virahol that step 02 and step 04 reclaim can recycle for step 01.
Embodiment 4:
Step 01: with tea waste pulverize dry after, take by weighing tea waste powder 75 grams, put into 500 milliliters apparatus,Soxhlet's, add 225 milliliters of the isopropanol extraction agent of moisture content 0.1%, the refluxing extraction of heating 1.5 hours;
Step 02: with isolated all extracting solution distillations in step 01 apparatus,Soxhlet's, reclaim extraction agent Virahol 160ml, the residue after the distillation is thick tea saponin;
Step 03: the thick tea saponin of step 02 gained is moved in the furnace pot, after water-bath is heated to constant weight, obtain refined theasapogenin 9.3 grams, gained tea saponin purity is greater than 95%;
Step 04: with the residue heating in water bath that retains in step 01 apparatus,Soxhlet's, steam the extraction agent Virahol that remains in the residue, reclaim Virahol 45ml, the Virahol that step 02 and step 04 reclaim can recycle for step 01.
Embodiment 5:
Step 01: with tea waste pulverize dry after, take by weighing tea waste powder 75 grams, put into 500 milliliters apparatus,Soxhlet's, add 190 milliliters of the isopropanol extraction agent of moisture content 0.2%, the refluxing extraction of heating 1.5 hours;
Step 02: with isolated all extracting solution distillations in step 01 apparatus,Soxhlet's, reclaim extraction agent Virahol 140ml, the residue after the distillation is thick tea saponin;
Step 03: the thick tea saponin of step 02 gained is moved in the furnace pot, after water-bath is heated to constant weight, obtain refined theasapogenin 9.1 grams, gained tea saponin purity is greater than 96%;
Step 04: with the residue heating in water bath that retains in step 01 apparatus,Soxhlet's, steam the extraction agent Virahol that remains in the residue, reclaim Virahol 38ml, the Virahol that step 02 and step 04 reclaim can recycle for step 01.
During experiment, before embodiment 1-5 step 01, with industrial naptha the Residual oil in the tea waste powder is leached, the organic acid in the Virahol is removed.
Through the experiment comparative analysis as can be known, in the step 01 reflux extracting time be 1.5 hours for best, the best when tea waste powder and extraction agent weightmeasurement ratio are 3:8.
The above only is embodiments of the invention; be not so limit claim of the present invention; every equivalent structure or equivalent flow process conversion that utilizes description of the present invention to do; or directly or indirectly be used in other relevant technical fields, all in like manner be included in the scope of patent protection of the present invention.
Claims (6)
1. method of from tea-seed pancake, extracting tea saponin, it is characterized in that: it may further comprise the steps:
Step 01: with tea waste pulverize dry after, take by weighing the tea waste powder of a weight part, put into apparatus,Soxhlet's, add the isopropanol extraction agent of one to three weight volume ratio, the refluxing extraction of heating 1-2 hour;
Step 02: with isolated all extracting solution distillations in step 01 apparatus,Soxhlet's, reclaim the extraction agent Virahol, the residue after the distillation is thick tea saponin;
Step 03: the thick tea saponin of step 02 gained is moved in the furnace pot, after water-bath is heated to constant weight, obtain refined theasapogenin;
Step 04: with the residue heating in water bath that retains in step 01 apparatus,Soxhlet's, steam the extraction agent Virahol that remains in the residue, the Virahol that step 02 and step 04 reclaim can recycle for step 01.
2. a kind of method of extracting tea saponin from tea-seed pancake according to claim 1, it is characterized in that: the water content of Virahol is less than or equal to 0.2% in the described step 01.
3. a kind of method of extracting tea saponin from tea-seed pancake according to claim 1 is characterized in that: in the described step 01 reflux extracting time be 1.5 hours for best.
4. a kind of method of extracting tea saponin from tea-seed pancake according to claim 1 is characterized in that: best when tea waste powder and extraction agent weightmeasurement ratio are 3:8 in the described step 01.
5. a kind of method of extracting tea saponin from tea-seed pancake according to claim 1 is characterized in that: before described step 01, with industrial naptha the Residual oil in the tea waste powder is leached.
6. a kind of method of extracting tea saponin from tea-seed pancake according to claim 1 is characterized in that: before described step 01, the organic acid in the Virahol is removed.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106083984A (en) * | 2016-06-26 | 2016-11-09 | 周荣 | A kind of method from extracting tea saponin from tea seed meal |
| CN106188214A (en) * | 2016-07-19 | 2016-12-07 | 陕西科技大学 | A kind of simple and easy method purifying tea saponin |
| CN106188213A (en) * | 2016-07-19 | 2016-12-07 | 陕西科技大学 | A kind of Simple Purification method of tea saponin |
| CN108997467A (en) * | 2018-07-11 | 2018-12-14 | 永州职业技术学院 | A method of Tea Saponin is extracted using biotechnology |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102250681A (en) * | 2011-06-20 | 2011-11-23 | 江南大学 | Method for extracting camellia oil and tea saporin from tea seeds |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102250681A (en) * | 2011-06-20 | 2011-11-23 | 江南大学 | Method for extracting camellia oil and tea saporin from tea seeds |
Non-Patent Citations (2)
| Title |
|---|
| 汪多仁: "茶皂素的开发与应用进展", 《染整技术》, vol. 33, no. 6, 30 June 2011 (2011-06-30), pages 39 - 47 * |
| 赖建辉: "茶籽的综合利用", 《茶叶通报》, no. 3, 31 December 1990 (1990-12-31), pages 33 - 38 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106083984A (en) * | 2016-06-26 | 2016-11-09 | 周荣 | A kind of method from extracting tea saponin from tea seed meal |
| CN106083984B (en) * | 2016-06-26 | 2018-02-13 | 福建三九军大生物科技有限公司 | A kind of method from extracting tea saponin from tea seed meal |
| CN106188214A (en) * | 2016-07-19 | 2016-12-07 | 陕西科技大学 | A kind of simple and easy method purifying tea saponin |
| CN106188213A (en) * | 2016-07-19 | 2016-12-07 | 陕西科技大学 | A kind of Simple Purification method of tea saponin |
| CN106188214B (en) * | 2016-07-19 | 2018-04-06 | 陕西科技大学 | A kind of simple and easy method for purifying Tea Saponin |
| CN108997467A (en) * | 2018-07-11 | 2018-12-14 | 永州职业技术学院 | A method of Tea Saponin is extracted using biotechnology |
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Denomination of invention: Method for extracting tea saponin from tea-seed pancake Effective date of registration: 20151201 Granted publication date: 20131204 Pledgee: Fujian Shunchang state owned assets Klc Holdings Ltd Pledgor: LAO ZHI QIN GROUP CO., LTD. Registration number: 2015350000091 |
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