CN102864068A - Device for fast screening and culturing anaerobic ammonia oxidation microbial communities - Google Patents
Device for fast screening and culturing anaerobic ammonia oxidation microbial communities Download PDFInfo
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Abstract
The invention relates to the technical field of the wastewater biological treatment, and in particular relates to a device for fast screening and culturing anaerobic ammonia oxidation (Anammox) microbial communities. The device comprises an anaerobic reactor, an inner pressure tube type ultrafiltration membrane, a water inlet controlling device, an automatic pH regulating device, an equilibrium reagent-supplementing device, a pneumatic pressure regulation device and an electronic control automatic thermostatic device, wherein the anaerobic reactor is arranged in the electronic control automatic thermostatic device and connected with the inner pressure tube type ultrafiltration membrane, the automatic pH regulating device, the equilibrium reagent-supplementing device, the water inlet controlling device and the pneumatic pressure regulation device respectively. Compared with the prior art, the device for fast screening and culturing Anammox microbial communities can provide an environment for the rapid proliferation of Anammox, thus shortening the doubling time and increasing the growth rate of Anammox.
Description
Technical field
The present invention relates to the biological wastewater treatment technology field, particularly a kind of rapid screening is cultivated the anaerobic ammonia oxidation microbiological pool device.
Background technology
Nineteen ninety, anaerobic ammonia oxidation process is developed in the Dutch Delft Kluyver of technology university Biotechnology Experiment chamber, and under anaerobic namely, microorganism is directly with NH
4 +Do electron donor, with NO
2 -Be electron acceptor(EA), with NH
4 +Or NO
2 -Be transformed into N
2Biological oxidation process.Because anaerobic ammonium oxidation process utilizes the autotrophic type microorganism to finish, therefore not needing to add in addition COD supports denitrification, compares with conventional denitrification process and can save 100% carbon source.And if the preposition nitrifying process of anaerobic ammonium oxidation process and is combined, nitrifying process only need to be with part NH so
4 +Be oxidized to NO
2 --N, the oxygen-supplying amount of the comparable complete nitrification saving 62.5% of such short distance nitration and 50% alkali consumption.Sharon-Anammox (nitrosification-Anammox) process quilt for the treatment of anaerobic nitrification mud parting liquid and first Application in the Dokhaven of Rotterdam, NED sewage work.Owing to carrying out anaerobic digestion after excess sludge is concentrated, the ammonia in the mud parting liquid is dense, and (approximately 1200～2000mg/L), therefore, this sewage work has adopted Sharon-Anammox technique, and has obtained good ammonia nitrogen removal effect again.
Anaerobic ammonia oxidizing bacteria (anaerobic ammonium oxidation, be called for short the Anammox bacterium) be a bacterioid, belong to floating mould door, comprise (Candidatus Brocadia), (Candidatus Kuenenia) and, Candidatus " Anammoxoglobus ", Candidatus " Jettenia ", (Candidatus Scalindua) belongs to.They fail so far to separate and obtain pure bacterial strain, therefore not yet obtain definite designation and classification.But in practical engineering application, they can be in anaerobic environment, with ammonium ion (NH
4 +) usefulness nitrite anions (NO
2 -) be oxidized to nitrogen:
NH
4 ++NO
2 -→N
2+2H
2O,ΔG=-357kJ?mol
In anaerobic ammonium oxidation process, azanol and hydrazine are the intermediate of metabolic process.The same with other floating mould door bacterium, anaerobic ammonia oxidizing bacteria also has membrane structure in the cell, and wherein, the capsule that carries out the ammonia anaerobic oxidation is called Anammoxosome (anammoxozome), and small molecules and poisonous hydrazine generate in this Anammoxosome.The film fat of Anammoxosome has special terraced alkane (ladderane) structure, can stop hydrazine to leak, thereby takes full advantage of chemical energy to avoid hydrazine to poison cell.
The requirement of condition (pH value, temperature, dissolved oxygen etc.) is relatively harsher to external world usually in the reaction of ammonia nitrogen in the anaerobic ammonia oxidizing bacteria family degradation of sewage, but because this reaction does not need oxygen and organic participation, therefore the exploitation of its research and technique had the meaning of Sustainable development.Anaerobic ammonia oxidizing bacteria family is used to the sewage purification of high ammonia nitrogen, low carbon source at present at commercial field, because its ammonia in can less energy-consumption ground water of decomposition.Significant to global nitrogen cycle, also be bacterium important in the sewage disposal.
At present, the cultivation difficulty that engineering is used upper Anammox flora is larger, and the speed of growth of Anammox bacterium is slow, and the doubling time is long, and cultivating under the general nature condition needs 200d～1000d or longer time to use.Therefore, adopt the artificial-strengthening measure to cultivate in a short time the Anammox flora, make it form rapidly dominant microflora significant on engineering is used.
Summary of the invention
The object of the invention is to provide a kind of rapid screening to cultivate the anaerobic ammonia oxidation microbiological pool device, the cultivation difficulty of using upper Anammox flora with engineering in the solution prior art is larger, the speed of growth of Anammox bacterium is slow, doubling time is long, cultivates the technical matters that need 200d～1000d or longer time to use under the general nature condition.
The object of the invention is achieved through the following technical solutions:
A kind of rapid screening is cultivated the anaerobic ammonia oxidation microbiological pool device, comprises anaerobic reactor, interior pressure-pipe type ultra-filtration membrane, inlet control device, pH automatic-adjusting device, balance medicament supplementary device, barometric control unit and electric control automatic constant temperature device, wherein,
Anaerobic reactor is used for cultivating the anaerobic ammonia oxidation microbiological flora;
Interior pressure-pipe type ultra-filtration membrane is used for the control water outlet and hydraulic mixing is provided;
Inlet control device, the water inlet that is used for removing the oxygen of water inlet and controls anaerobic reactor;
The pH automatic-adjusting device is used for automatically regulating the pH value in the anaerobic reactor, makes the interior pH value of anaerobic reactor maintain the scope of suitable anaerobic ammonia oxidation microbiological flora breeding;
Additional providing into the required electron donor deficiency of water ammonia nitrogen oxidation is provided balance medicament supplementary device, mainly adopts nitrite, makes ammonium ion (NH in the anaerobic reactor
4 +) and nitrite anions (NO
2 -) the equivalent ratio kept about 1: 1;
Barometric control unit is used for regulating the air pressure of anaerobic reactor top, and guarantees to enter the air anaerobic in the anaerobic reactor;
The electric control automatic constant temperature device is used for regulating the temperature in the anaerobic reactor, makes the interior temperature of anaerobic reactor maintain the scope of suitable anaerobic ammonia oxidation microbiological flora breeding;
Described anaerobic reactor is arranged in the described electric control automatic constant temperature device, and described anaerobic reactor is connected with barometric control unit with interior pressure-pipe type ultra-filtration membrane, pH automatic-adjusting device, balance medicament supplementary device, inlet control device respectively.
Preferably, the medium position of described anaerobic reactor is provided with water absorption tube, the bottom position of described anaerobic reactor is provided with perforated pipe, and described water absorption tube is connected with the water-in of described interior pressure-pipe type ultra-filtration membrane, and described perforated pipe is connected with the water outlet of described interior pressure-pipe type ultra-filtration membrane.
Preferably, described perforated pipe side is provided with some through holes.
Preferably, described electric control automatic constant temperature device comprises the water-bath intraformational bed, and described water-bath intraformational bed is set in the described anaerobic reactor outside.
Preferably, also comprise thermal insulation layer, described water-bath intraformational bed and described anaerobic reactor are sealed in the described thermal insulation layer.
Preferably, described barometric control unit comprises the deaerated water seal apparatus at the top that is arranged on described anaerobic reactor and unidirectionally goes out gas device, the escape pipe of described deaerated water seal apparatus is connected with described anaerobic reactor, and the described unidirectional air intake that goes out gas device is connected with described anaerobic reactor.
Preferably, the oxygen scavenger in the described deaerated water seal apparatus is sodium sulfite solution, is provided with inlet pipe and described escape pipe in the described deaerated water seal apparatus.
Preferably, described inlet control device comprises that the water outlet of the biological pre-deoxygenation regulating tank of described water inlet is connected with described anaerobic reactor into the biological pre-deoxygenation regulating tank of water.
Preferably, the pH adjusting agent in the described pH automatic-adjusting device is hydrocarbonate.
Preferably, described balance medicament supplementary device detects real-time ammonia-nitrogen content and the nitrite nitrogen content that provides according to the online ammonia nitrogen of water inlet, replenishes in real time nitrite, makes ammonium ion (NH in the anaerobic reactor
4 +) and nitrite anions (NO
2 -) the equivalent ratio kept about 1: 1.
Compared with prior art, the present invention has following advantage:
1, rapid screening of the present invention is cultivated the anaerobic ammonia oxidation microbiological pool device environment that makes Anammox bacterium fast breeding can be provided, thereby shortens the doubling time, improves the speed of growth of Anammox bacterium;
2, anaerobic reactor adopts external interior pressure-pipe type ultra-filtration membrane cross-flow (crossflow) operation control water outlet, not only control is run off, and can utilize cross-flow circulation mixed solution that the anaerobic system hydraulic mixing is provided, whole service link aquifer yield can be regulated and control, and sneaks into without any other oxygen-containing gas; Simultaneously, because anaerobic reactor inside does not have mechanical means, so that the maintenance cost of system is lower, convenient management.3, at anaerobic reactor periphery and bottom the water-bath intraformational bed is set, and adopts the sealing of thermal insulation material thermal insulation layer in the outside of whole reactive system, can keep better the stable of temperature;
4, adopt the biological pre-deoxygenation regulating tank of water inlet to remove oxygen in the water, regulating tank is the sealed shell of tank that gas barrier is established at the top, calculating hydraulic detention time according to flooding velocity is between 30min～60min, utilizes the trace dissolved oxygen approach exhaustion during residual aerobic flora will be intake in the waste water; And when medicament is regulated in preparation, adopt the airtight configuration measure of deaerated water, the control water inlet is substantially without dissolved oxygen (dissolved oxygen content is lower than 0.1mg/L);
5, be provided with deaerated water seal apparatus and unidirectionally go out gas device at the anaerobic reactor top, guarantee inside reactor gas blanket anaerobic; Automatically controlling under the Inlet and outlet water condition, system's upper space changes not quite substantially, mainly is the N that reactor produces
2Need in time to derive with a small amount of rare gas element, therefore, the reagent consumption of deaerated water seal apparatus is very little, only needs to make regular check on replacing deoxygenation S-WAT (sodium sulfite) solution and gets final product;
6, adopt hydrocarbonate as pH adjusting agent, add by automatic control, make the interior pH value of anaerobic reactor can be controlled at optimum range.
7, for the ammonium ion (NH in the preposition water inlet
4 +) and nitrite anions (NO
2 -) proportion requirement is not high, by balance medicament supplementary device, detects real-time ammonia-nitrogen content and the nitrite nitrogen content that provides according to the online ammonia nitrogen of water inlet, replenishes in real time nitrite, makes ammonium ion (NH in the anaerobic reactor
4 +) and nitrite anions (NO
2 -) the equivalent ratio keep 1: 1 left and right sides optimum range.
Description of drawings
Fig. 1 is the structural representation that rapid screening of the present invention is cultivated the anaerobic ammonia oxidation microbiological pool device.
Embodiment
Below in conjunction with accompanying drawing, describe the present invention in detail.
See also Fig. 1, rapid screening of the present invention is cultivated the anaerobic ammonia oxidation microbiological pool device, comprise anaerobic reactor 1, interior pressure-pipe type ultra-filtration membrane 4, inlet control device 7, pH automatic-adjusting device, balance medicament supplementary device, barometric control unit and electric control automatic constant temperature device, anaerobic reactor 1 is arranged in the electric control automatic constant temperature device, and anaerobic reactor 1 is connected with barometric control unit with interior pressure-pipe type ultra-filtration membrane 4, pH automatic-adjusting device, balance medicament supplementary device, inlet control device 7 respectively.
The medium position of anaerobic reactor 1 is provided with water absorption tube 11, the bottom position of anaerobic reactor 1 is provided with perforated pipe 12, water absorption tube 11 is connected with the water-in of interior pressure-pipe type ultra-filtration membrane 4 after anaerobic reactor stretches out, perforated pipe 12 is connected with the water outlet of interior pressure-pipe type ultra-filtration membrane 4 after anaerobic reactor stretches out, and the side of perforated pipe 12 is provided with some through holes.Utilize the cross-flow circulation to spray carrying out hydraulic mixing in the anaerobic reactor from bottom perforated pipe 12.Stirring mainly contains two effects, and one is to utilize high velocity flow and water pump impeller shearing action that the Anammox flora is disperseed, and opens the surface in contact of thalline and water, makes the surperficial mass-transfer efficiency of microbiological treatment the highest; Another effect is that device internal mix liquid is fully stirred, and strengthens convective mass transfer.Because anaerobic reactor inside does not have mechanical means, so that the maintenance cost of system is lower, convenient management.
The electric control automatic constant temperature device comprises water-bath intraformational bed 2, and water-bath intraformational bed 2 is set in anaerobic reactor 1 outside.Water-bath intraformational bed 2 forms waterway circulating with the water pipeline of outside, and outside water pipeline is provided with well heater.Rapid screening of the present invention is cultivated the anaerobic ammonia oxidation microbiological pool device and is also comprised thermal insulation layer 3, and water-bath intraformational bed 2 and anaerobic reactor 1 are sealed in the thermal insulation layer 3.Thermal insulation layer 3 adopts thermal insulation material to make.The present invention by water-bath intraformational bed and thermal insulation layer can so that the temperature of anaerobic reactor inside can more stably remain in the suitable scope.
Barometric control unit comprises the deaerated water seal apparatus 5 at the top that is arranged on anaerobic reactor and unidirectionally goes out gas device 6, and deaerated water seal apparatus 5 is used for to the anaerobic reactor make-up gas, unidirectionally goes out gas device 6 and is used for N that anaerobic reactor is produced
2In time derive with a small amount of rare gas element.The escape pipe of deaerated water seal apparatus is connected with anaerobic reactor, and the unidirectional air intake that goes out gas device is connected with anaerobic reactor.Oxygen scavenger in the deaerated water seal apparatus is sodium sulfite solution, is provided with inlet pipe and escape pipe in the deaerated water seal apparatus.Automatically controlling under the Inlet and outlet water condition, system's upper space changes not quite substantially, mainly is the N that reactor produces
2Need in time to derive with a small amount of rare gas element, therefore, the reagent consumption of deaerated water seal apparatus is very little, only needs to make regular check on replacing deoxygenation S-WAT (sodium sulfite) solution and gets final product.
PH automatic-adjusting device of the present invention is used for automatically regulating the pH value in the anaerobic reactor, and the pH adjusting agent in the pH automatic-adjusting device is hydrocarbonate.Adopt hydrocarbonate as pH adjusting agent, add by automatic control, make the interior pH value of anaerobic reactor can be controlled at optimum range, and, regulate the pH value by hydrocarbonate, can so that pH value scope is more stable, change little.
Balance medicament supplementary device of the present invention can detect real-time ammonia-nitrogen content and the nitrite nitrogen content that provides according to the online ammonia nitrogen of water inlet, adds the pump interlock by metering, automatically replenishes in real time nitrite, makes ammonium ion (NH in the anaerobic reactor
4 +) and nitrite anions (NO
2 -) the equivalent ratio kept about 1: 1, provide anaerobic ammonia oxidation microbiological flora Fast-propagation needed balanced nutrients supply conditions.
During use, first the middle lower floor of the anaerobic sludge of sewage work or refuse landfill equalizing tank is oozed drop liquid and put into anaerobic reactor as the inoculation mother liquor; Then the deaerated water after the biological pre-deoxygenation regulating tank of will intaking is processed passes into anaerobic reactor, makes liquid level rise to effective liquid level of anaerobic reactor; With the pH automatic-adjusting device pH value of anaerobic reactor internal reaction liquid is transferred to 7.5-8 again; With automatically controlled automatic thermostatic device the temperature of water-bath in the water-bath intraformational bed is transferred to 38 ℃ again; Pressure-pipe type ultra-filtration membrane control water outlet is played stirring action to the reaction solution of anaerobic reactor simultaneously in starting; The N that anaerobic reactor produces
2Go out gas device and derive from unidirectional with a small amount of rare gas element, air is passed in the deaerated water seal apparatus, the air after deoxygenation enters into anaerobic reactor to play the effect of regulating pressure; By real-time ammonia-nitrogen content and the nitrite nitrogen content that online ammonia nitrogen detects to be provided of intaking, utilize metering to add the pump interlock, automatically replenish in real time nitrite, make ammonium ion (NH in the anaerobic reactor
4 +) and nitrite anions (NO
2 -) the equivalent ratio kept about 1: 1, provide anaerobic ammonia oxidation microbiological flora Fast-propagation needed balanced nutrients supply conditions.
Rapid screening of the present invention is cultivated the anaerobic ammonia oxidation microbiological pool device can provide the environment that makes Anammox bacterium fast breeding, thereby shortens the doubling time, improves the speed of growth of Anammox bacterium.
Above disclosed only be several specific embodiments of the application, but the application is not limited thereto, the changes that any person skilled in the art can think of all should drop in the application's the protection domain.
Claims (10)
1. a rapid screening is cultivated the anaerobic ammonia oxidation microbiological pool device, it is characterized in that, comprise anaerobic reactor, interior pressure-pipe type ultra-filtration membrane, inlet control device, pH automatic-adjusting device, balance medicament supplementary device, barometric control unit and electric control automatic constant temperature device, wherein
Anaerobic reactor is used for cultivating the anaerobic ammonia oxidation microbiological flora;
Interior pressure-pipe type ultra-filtration membrane is used for the control water outlet and hydraulic mixing is provided;
Inlet control device, the water inlet that is used for removing the oxygen of water inlet and controls anaerobic reactor;
The pH automatic-adjusting device is used for automatically regulating the pH value in the anaerobic reactor, makes the interior pH value of anaerobic reactor maintain the scope of suitable anaerobic ammonia oxidation microbiological flora breeding;
Additional providing into the required electron donor deficiency of water ammonia nitrogen oxidation is provided balance medicament supplementary device, mainly adopts nitrite, makes ammonium ion (NH in the anaerobic reactor
4 +) and nitrite anions (NO
2 -) the equivalent ratio kept about 1: 1;
Barometric control unit is used for regulating the air pressure of anaerobic reactor top, and guarantees to enter the air anaerobic in the anaerobic reactor;
The electric control automatic constant temperature device is used for regulating the temperature in the anaerobic reactor, makes the interior temperature of anaerobic reactor maintain the scope of suitable anaerobic ammonia oxidation microbiological flora breeding;
Described anaerobic reactor is arranged in the described electric control automatic constant temperature device, and described anaerobic reactor is connected with barometric control unit with interior pressure-pipe type ultra-filtration membrane, pH automatic-adjusting device, balance medicament supplementary device, inlet control device respectively.
2. a kind of rapid screening as claimed in claim 1 is cultivated the anaerobic ammonia oxidation microbiological pool device, it is characterized in that, the medium position of described anaerobic reactor is provided with water absorption tube, the bottom position of described anaerobic reactor is provided with perforated pipe, described water absorption tube is connected with the water-in of described interior pressure-pipe type ultra-filtration membrane, and described perforated pipe is connected with the water outlet of described interior pressure-pipe type ultra-filtration membrane.
3. a kind of rapid screening as claimed in claim 2 is cultivated the anaerobic ammonia oxidation microbiological pool device, it is characterized in that, described perforated pipe side is provided with some through holes.
4. a kind of rapid screening as claimed in claim 1 is cultivated the anaerobic ammonia oxidation microbiological pool device, it is characterized in that, described electric control automatic constant temperature device comprises the water-bath intraformational bed, and described water-bath intraformational bed is set in the described anaerobic reactor outside.
5. a kind of rapid screening as claimed in claim 4 is cultivated the anaerobic ammonia oxidation microbiological pool device, it is characterized in that, also comprise thermal insulation layer, described water-bath intraformational bed and described anaerobic reactor are sealed in the described thermal insulation layer.
6. a kind of rapid screening as claimed in claim 1 is cultivated the anaerobic ammonia oxidation microbiological pool device, it is characterized in that, described barometric control unit comprises the deaerated water seal apparatus at the top that is arranged on described anaerobic reactor and unidirectionally goes out gas device, the escape pipe of described deaerated water seal apparatus is connected with described anaerobic reactor, and the described unidirectional air intake that goes out gas device is connected with described anaerobic reactor.
7. a kind of rapid screening as claimed in claim 6 is cultivated the anaerobic ammonia oxidation microbiological pool device, it is characterized in that, the oxygen scavenger in the described deaerated water seal apparatus is sodium sulfite solution, is provided with inlet pipe and described escape pipe in the described deaerated water seal apparatus.
8. a kind of rapid screening as claimed in claim 1 is cultivated the anaerobic ammonia oxidation microbiological pool device, it is characterized in that, described inlet control device comprises that the water outlet of the biological pre-deoxygenation regulating tank of described water inlet is connected with described anaerobic reactor into the biological pre-deoxygenation regulating tank of water.
9. a kind of rapid screening as claimed in claim 1 is cultivated the anaerobic ammonia oxidation microbiological pool device, it is characterized in that, the pH adjusting agent in the described pH automatic-adjusting device is hydrocarbonate.
10. a kind of rapid screening as claimed in claim 1 is cultivated the anaerobic ammonia oxidation microbiological pool device, it is characterized in that, described balance medicament supplementary device detects real-time ammonia-nitrogen content and the nitrite nitrogen content that provides according to the online ammonia nitrogen of water inlet, replenish in real time nitrite, make ammonium ion (NH in the anaerobic reactor
4 +) and nitrite anions (NO
2 -) the equivalent ratio kept about 1: 1.
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| CN111732192A (en) * | 2020-06-24 | 2020-10-02 | 盐城工学院 | Short-cut nitrification-anaerobic ammonia oxidation denitrification process for convective distribution of water inflow |
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| CN114344949A (en) * | 2021-12-28 | 2022-04-15 | 安徽国孚润滑油工业有限公司 | Extraction raffinate oil adsorption device and method for circulating extraction raffinate oil spiral impact type auxiliary silica gel discharge |
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