CN102851333A - Method for synthesizing beta-alanine by biological catalysis - Google Patents
Method for synthesizing beta-alanine by biological catalysis Download PDFInfo
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- CN102851333A CN102851333A CN2012100751340A CN201210075134A CN102851333A CN 102851333 A CN102851333 A CN 102851333A CN 2012100751340 A CN2012100751340 A CN 2012100751340A CN 201210075134 A CN201210075134 A CN 201210075134A CN 102851333 A CN102851333 A CN 102851333A
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- alanine
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Abstract
The invention provides a method for synthesizing beta-alanine by biological catalysis, and belongs to the field of enzyme engineering. The method comprises the following steps: culturing escherichia coli microbe to obtain aspartase and L-aspartic acid-alpha-decarboxylase, mixing the enzyme-containing escherichia coli cells with an ammonium fumarate aqueous solution, performing an enzyme reaction to generate beta-alanine. The method of the invention is cheap and easily available in raw materials, simple in production process, safe, environment-friendly, mild in reaction condition, high in efficiency, low in production cost, and high in product quality, and has significant economic benefits and environmental protection benefits when compared with traditional methods.
Description
Technical field
The present invention relates to the method for the synthetic Beta-alanine of a kind of biocatalysis, belong to the enzyme engineering field.
Background technology
Beta-alanine (English name beta-Alanine) has another name called β-alanine, and it is a kind of nonprotein amino acid, is the unique β type amino acid of occurring in nature, is found in the degraded product of uridylic by Ross and Monroe in 1972.Beta-alanine is a kind of important biochemical raw material, in medicine, feed and field of food have a wide range of applications field and market outlook.At present, Beta-alanine be mainly used in synthetic pantothenic acid and calcium pantothenate, pantothenic acid is known as again vitamins B
5, be the integral part of the necessary coenzyme A of multiple metabolism.
Synthetic method according to present bibliographical information Beta-alanine mainly contains following several:
1, chemical synthesis
(1) propylene acid system (US 2376334 A, 1945.05.22; US 3105092 A, 1963.09.24; US 3846489 A, 1974.11.05): vinylformic acid, acrylate or acrylate and ammoniacal liquor, issue the ammonifying reaction at higher temperature and pressure, obtain Beta-alanine.Propylene acid system by product is many, needs High Temperature High Pressure, and the corrodibility of vinylformic acid itself is very strong, so reaction having relatively high expectations to equipment.
(2) vinyl cyanide method (US 2335997 A, 1943.12.07; US 2377401 A, 1947.06.14): vinyl cyanide and ammonia react under High Temperature High Pressure and generate β-aminopropionitrile, and then hydrolysis reaction generates Beta-alanine under acidity or alkaline condition.The vinyl cyanide method is used the vinyl cyanide of severe toxicity, safety precaution had relatively high expectations, and the reaction needed High Temperature High Pressure, also higher to equipment requirements, reaction yield is low, owing to generating a large amount of inorganic salt in the hydrolytic process, the product purification is difficulty comparatively, product purity is not high.
(3) β-aminopropionitrile method (US 2336067 A, 1943.12.07; J.Am.Chem.Soc.1945,67,876～877): β-aminopropionitrile is hydrolyzed under acidity or alkaline condition and generates Beta-alanine.The characteristics of β-aminopropionitrile method are that reaction yield is high, and shortcoming is that the price of β-aminopropionitrile is higher, causes production cost too high, can produce a large amount of salt in the N-process in addition, cause and extract difficulty.
2, biological synthesis process
(1) vinylformic acid ammonification enzyme process (CN 1285730 C, 2006.11.22): first culturing micro-organisms produces the amination enzyme, then the amination enzyme is added in the reaction solution of the aqueous solution of olefin(e) acid and ammonia, synthetic Beta-alanine under the effect of enzyme, behind deamination, purifying the Beta-alanine product.The method reaction efficiency is high, and cost is low, but raw material propylene acid is severe corrosive and pungency liquid, to having relatively high expectations of personal security and equipment, and present report there are no commercial application.
(2) L-Aspartic acid-α-decarboxylation enzyme process (CN 1242054 A, 2006.01.19): take the DL-aspartic acid as raw material, under the effect of L-Aspartic acid-α-decarboxylation enzyme process, generate D-Asp and Beta-alanine, then regulate the pH value and make the D-Asp crystallization, thereby obtain Beta-alanine.The method Beta-alanine contains a small amount of D-Asp, and the purity of product is not high; L-Aspartic acid-α-decarboxylase activity is low, and the efficient of enzymatic reaction is low, and raw material DL-aspartic acid price is higher, causes the Beta-alanine cost higher.
(3) nitrilase method (JP Unexamined Patent 10-42886 A, 2006.02.17): utilize the synthetic Beta-alanine of the microorganism catalysis β-aminopropionitrile of producing the organic nitrile lytic enzyme.This method substrate β-aminopropionitrile price is high, and reaction density is low, and production cost is relatively high, deficiency in economic performance.
Summary of the invention
The invention provides the method for a kind of safety, environmental protection, the synthetic Beta-alanine of biocatalysis that efficient, cost is low, product quality is high, thoroughly stopped severe toxicity in the traditional processing technology, severe corrosive, strong acid and strong base and the at high price use of raw material.
The present invention reaches the technical scheme that goal of the invention adopts:
Microorganism of the present invention is the Colibacter bacterial strain, pure culture on substratum, obtain two kinds of enzymes: L-Aspartase and L-Aspartic acid-α-decarboxylase, fumaric acid is dissolved in the substrate-ammonium fumarate solution that is mixed with reaction in the ammoniacal liquor, the Bacillus coli cells that will contain enzyme adds in the substrate and carries out enzymatic reaction, thereby generates Beta-alanine.
The used seed culture medium of the present invention is: fumaric acid 1～10g/L, Dried Corn Steep Liquor Powder 10～20g/L, polypeptide protein 5～15g/L, monosodium glutamate 5～10g/L, MgSO
47H
2O 0.2～0.5g/L, K
2HPO
40.15～0.25g/L, NaCl 0.1～0.5g/L transfers pH6.8～7.5 with ammoniacal liquor; Fermention medium is: fumaric acid 5～15g/L, Dried Corn Steep Liquor Powder 10～20g/L, polypeptide protein 5～15g/L, L-Aspartic acid 5～15g/L, beet alkali hydrochlorate 0.1～0.5g/L, MgSO
47H
2O 0.2～0.5g/L, K
2HPO
40.15～0.25g/L, NaCl 0.1～0.5g/L transfers pH6.8～7.5 with ammoniacal liquor.
Colibacillary culture temperature of the present invention is 28～45 ℃, rotating speed 150～300r/min, incubation time 16～32h.
The pH of substrate of the present invention is 6.0～7.5, and the concentration of fumaric acid is 50～150g/L in the substrate.
The addition of Bacillus coli cells is 20～50g/L in the enzymatic reaction of the present invention.
The temperature of enzymatic reaction of the present invention is 32～50 ℃, and pH is 6.0～7.5.
The present invention adopts stream to add fumaric acid and controls pH in the reaction process.
The method of the synthetic Beta-alanine of a kind of biocatalysis of the present invention, its beneficial effect is mainly reflected in:
(1) do not use severe toxicity, severe corrosive, strong acid and strong base raw material, production technique safety and environmental protection in the reaction process;
(2) reaction directly generates Beta-alanine, and is simple for process;
(3) temperature is 32～50 ℃ in the reaction process, and pH is 6.0～7.5, and reaction conditions is gentle;
(4) no coupling product and a large amount of salt produce in the reaction, and extraction process is simple, and product purity is high;
(5) the starting material fumaric acid is cheap and easy to get, and concentration of substrate is high, and productive rate is high, and production cost is low.
Embodiment
The invention will be further described below in conjunction with specific embodiment, but protection scope of the present invention is not limited only to this.
Embodiment 1: the mensuration of content
(1) mensuration of fumaric acid content (high performance liquid chromatography)
Enzymatic reaction solution 10000r/min centrifugation cell is got supernatant liquor and is diluted 10 times, prepares simultaneously fumaric acid standard model (2mg/ml), carries out efficient liquid phase chromatographic analysis.
Post model: Alltech organic acid chromatographic column No.88645,250mm * 4.6mm
Moving phase: 25mmol/L KH
2PO
4, pH2.5
Sample size: 20 μ l
Column temperature: 35 ℃
Gradient mode: constant current 1.0ml/min
Detector: ultraviolet wavelength 210nm
Account form: external standard method
(2) mensuration of Beta-alanine content (high performance liquid chromatography)
Get respectively the Beta-alanine sample and the Beta-alanine standard model (2mg/ml) that extract oven dry, carry out efficient liquid phase chromatographic analysis.
Deriving method: accurately take by weighing the 0.3430g o-phthalaldehyde(OPA) and be dissolved in the 5ml dehydrated alcohol, then add the 0.1472g N-acetyl-L-cysteine, use borate buffer solution (pH=9.5) constant volume of 0.1mol/L to 25ml, preparation derivating agent lucifuge is for subsequent use.Add successively borate buffer solution (pH=9.5) 300 μ l, sample solution 250 μ l, derivating agent 250 μ l.Wait for strict period of 2min(and reagent addition behind the mixing), sample introduction then.
The neutral post of post model: XDB-C8,150mm * 4.6mm
Moving phase: A:0.05mol/L sodium-acetate buffer, B: methyl alcohol
Sample size: 20 μ l
Column temperature: 30 ℃
Gradient mode: mobile phase A: Mobile phase B=65:35, constant current 1.0ml/min
Detector: ultraviolet wavelength 334nm
Account form: external standard method
Embodiment 2: biocatalysis is synthesized Beta-alanine
Seed culture medium: fumaric acid 5g, Dried Corn Steep Liquor Powder 10g, polypeptide protein 10g, monosodium glutamate 10g, MgSO
47H
2O 0.2g, K
2HPO
40.15g NaCl 0.4g adds water and is mixed with the solution of 1L and transfers pH7.0 with ammoniacal liquor, 121 ℃ of steam sterilizing 20min.
Fermention medium: fumaric acid 20g, Dried Corn Steep Liquor Powder 40g, polypeptide protein 40g, L-Aspartic acid 20g, beet alkali hydrochlorate 0.8g, MgSO
47H
2O 0.8g, K
2HPO
40.6g NaCl 0.4g adds water and is mixed with the solution of 4L and transfers pH7.0 with ammoniacal liquor, 121 ℃ of steam sterilizing 20min.
Substrate solution: fumaric acid 100g, regulate pH7.5 with ammoniacal liquor, be settled to 1L.
Intestinal bacteria slant strains access seed culture medium, 200r/min, 37 ℃ of shaking tables are cultivated 24h.Inoculum size with 10% changes fermention medium over to, 200r/min, and 37 ℃ of shaking tables are cultivated 18h and are obtained fermented liquid, use whizzer under the 4000r/min rotating speed, centrifugal collection 126g intestinal bacteria wet cell.Get the colibacillary wet cell of 35g and add substrate solution, carry out enzymatic reaction, continue to flow and add the pH7.5 that fumaric acid is controlled reaction, pH keeps stable behind the reaction 18h, and stream adds fumaric acid 64g, continues reaction again, every interval 1h sampling once, detect fumaric acid content with HPLC, fumaric acid content＜0.1% in the detection reaction liquid behind the 4h, reaction is finished.Reaction solution is at the centrifugal removal Bacillus coli cells of 4000r/min, add activated carbon decolorizing, filter, be evaporated to 1/4 of original volume, under slow agitation condition, drip 3 times of volume dehydrated alcohols, have a large amount of white crystals to separate out, suction filtration goes out crystal and uses a small amount of absolute ethanol washing, and weighing gets white solid 101.8g after the oven dry, yield is 80.9%, and the content that detects Beta-alanine with HPLC is 99.2%.
Embodiment 3: biocatalysis is synthesized Beta-alanine
Seed culture medium: fumaric acid 10g, Dried Corn Steep Liquor Powder 15g, polypeptide protein 5g, monosodium glutamate 8g, MgSO
47H
2O 0.3g, K
2HPO
40.20g NaCl 0.25g adds water and is mixed with the solution of 1L and transfers pH7.2 with ammoniacal liquor, 121 ℃ of steam sterilizing 20min.
Fermention medium: fumaric acid 40g, Dried Corn Steep Liquor Powder 60g, polypeptide protein 20g, L-Aspartic acid 40g, beet alkali hydrochlorate 1.0g, MgSO
47H
2O 1.0g, K
2HPO
40.8g NaCl 1.0g adds water and is mixed with the solution of 4L and transfers pH7.2 with ammoniacal liquor, 121 ℃ of steam sterilizing 20min.
Substrate solution: fumaric acid 80g, regulate pH7.0 with ammoniacal liquor, be settled to 1L.
Intestinal bacteria slant strains access seed culture medium, 250r/min, 32 ℃ of shaking tables are cultivated 20h.Inoculum size with 10% changes fermention medium over to, 250r/min, and 32 ℃ of shaking tables are cultivated 24h and are obtained fermented liquid, use whizzer under the 4000r/min rotating speed, centrifugal collection 142g intestinal bacteria wet cell.Get the colibacillary wet cell of 25g and add substrate solution, carry out enzymatic reaction, continue to flow and add the pH7.0 that fumaric acid is controlled reaction, pH keeps stable behind the reaction 16h, and stream adds fumaric acid 68g, continues reaction again, every interval 1h sampling once, detect fumaric acid content with HPLC, fumaric acid content＜0.1% in the detection reaction liquid behind the 4h, reaction is finished.Reaction solution is at the centrifugal removal Bacillus coli cells of 4000r/min, add activated carbon decolorizing, filter, be evaporated to 1/4 of original volume, under slow agitation condition, drip 3 times of volume dehydrated alcohols, have a large amount of white crystals to separate out, suction filtration goes out crystal and uses a small amount of absolute ethanol washing, and weighing gets white solid 85.6g after the oven dry, yield is 75.4%, and the content that detects Beta-alanine with HPLC is 98.9%.
Embodiment 4: biocatalysis is synthesized Beta-alanine
Seed culture medium: fumaric acid 8g, Dried Corn Steep Liquor Powder 20g, polypeptide protein 15g, monosodium glutamate 5g, MgSO
47H
2O 0.5g, K
2HPO
40.25g NaCl 0.5g adds water and is mixed with the solution of 1L and transfers pH7.5 with ammoniacal liquor, 121 ℃ of steam sterilizing 20min.
Fermention medium: fumaric acid 60g, Dried Corn Steep Liquor Powder 80g, polypeptide protein 60g, L-Aspartic acid 60g, beet alkali hydrochlorate 2.0g, MgSO
47H
2O 2.0g, K
2HPO
41.0g NaCl 2.0g adds water and is mixed with the solution of 4L and transfers pH7.5 with ammoniacal liquor, 121 ℃ of steam sterilizing 20min.
Substrate solution: fumaric acid 140g, regulate pH6.5 with ammoniacal liquor, be settled to 1L.
Intestinal bacteria slant strains access seed culture medium, 300r/min, 41 ℃ of shaking tables are cultivated 18h.Inoculum size with 10% changes fermention medium over to, 300r/min, and 41 ℃ of shaking tables are cultivated 27h and are obtained fermented liquid, use whizzer under the 4000r/min rotating speed, centrifugal collection 161g intestinal bacteria wet cell.Get the colibacillary wet cell of 45g and add substrate solution, carry out enzymatic reaction, continue to flow and add the pH6.5 that fumaric acid is controlled reaction, pH keeps stable behind the reaction 25h, and stream adds fumaric acid 97g, continues reaction again, every interval 1h sampling once, detect fumaric acid content with HPLC, fumaric acid content＜0.1% in the detection reaction liquid behind the 6h, reaction is finished.Reaction solution is at the centrifugal removal Bacillus coli cells of 4000r/min, add activated carbon decolorizing, filter, be evaporated to 1/3 of original volume, under slow agitation condition, drip 3 times of volume dehydrated alcohols, have a large amount of white crystals to separate out, suction filtration goes out crystal and uses a small amount of absolute ethanol washing, and weighing gets white solid 155.3g after the oven dry, yield is 85.4%, and the content that detects Beta-alanine with HPLC is 99.0%.
Claims (7)
1. the method for the synthetic Beta-alanine of a biocatalysis, it is characterized in that the microorganism that the present invention adopts is the Colibacter bacterial strain, pure culture on substratum, obtain two kinds of enzymes: L-Aspartase and L-Aspartic acid-α-decarboxylase, fumaric acid is dissolved in the substrate-ammonium fumarate solution that is mixed with reaction in the ammoniacal liquor, the Bacillus coli cells that will contain enzyme adds in the substrate and carries out enzymatic reaction, thereby generates Beta-alanine.
2. the method for the synthetic Beta-alanine of a kind of biocatalysis according to claim 1 is characterized in that seed culture medium is: fumaric acid 1～10g/L, Dried Corn Steep Liquor Powder 10～20g/L, polypeptide protein 5～15g/L, monosodium glutamate 5～10g/L, MgSO
47H
2O 0.2～0.5g/L, K
2HPO
40.15～0.25g/L, NaCl 0.1～0.5g/L transfers pH6.8～7.5 with ammoniacal liquor; Fermention medium is: fumaric acid 5～15g/L, Dried Corn Steep Liquor Powder 10～20g/L, polypeptide protein 5～15g/L, L-Aspartic acid 5～15g/L, beet alkali hydrochlorate 0.1～0.5g/L, MgSO
47H
2O 0.2～0.5g/L, K
2HPO
40.15～0.25g/L, NaCl 0.1～0.5g/L transfers pH6.8～7.5 with ammoniacal liquor.
3. the method for the synthetic Beta-alanine of a kind of biocatalysis according to claim 1 is characterized in that colibacillary culture temperature is 28～45 ℃, rotating speed 150～300r/min, incubation time 16～32h.
4. the method for the synthetic Beta-alanine of a kind of biocatalysis according to claim 1 is characterized in that the pH of substrate is 6.0～7.5, and the concentration of fumaric acid is 50～150g/L in the substrate.
5. a kind of biocatalysis according to claim 1 is synthesized the method for Beta-alanine, and the addition that it is characterized in that Bacillus coli cells in the enzymatic reaction is 20～50g/L.
6. a kind of biocatalysis according to claim 1 is synthesized the method for Beta-alanine, and the temperature that it is characterized in that enzymatic reaction is 32～50 ℃, and pH is 6.0～7.5.
7. the method for the synthetic Beta-alanine of a kind of biocatalysis according to claim 1 or 5 is characterized in that utilizing stream to add fumaric acid and controls pH in the reaction process.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103320480A (en) * | 2013-06-25 | 2013-09-25 | 南京大学 | Method for preparing beta-alanine by coupled enzymatic reaction |
| CN103525880A (en) * | 2013-10-17 | 2014-01-22 | 张家港市华昌药业有限公司 | Application of maleic anhydride waste residue in preparation of aspartic acid and method for preparing aspartic acid by using maleic anhydride waste residue |
| CN103898035A (en) * | 2013-12-24 | 2014-07-02 | 安徽华恒生物科技股份有限公司 | Recombinant escherichia coli strain for producing beta-alanine as well as construction method and application thereof |
| CN104195193A (en) * | 2014-09-10 | 2014-12-10 | 中国科学院天津工业生物技术研究所 | Method for preparing beta-alanine through enzymatically catalyzing hydrolysis of high-concentration beta-aminopropionitrile |
| CN106755155A (en) * | 2016-12-07 | 2017-05-31 | 江南大学 | A kind of method for preparing beta Alanine with double enzyme series connection |
| CN110438039A (en) * | 2019-07-19 | 2019-11-12 | 郑州大学 | Bacterial strain is used in one plant of alanine fermentation |
| WO2020187256A1 (en) * | 2019-03-20 | 2020-09-24 | 广安摩珈生物科技有限公司 | Methods for preparing β-alanine, β-alanine salt and pantothenate |
| CN111718969A (en) * | 2019-03-20 | 2020-09-29 | 广安摩珈生物科技有限公司 | Process for producing beta-alanine |
| CN112481244A (en) * | 2020-12-08 | 2021-03-12 | 江南大学 | Aspartase mutant and coding gene, vector, recombinant bacterium and application thereof |
| CN113832201A (en) * | 2020-06-24 | 2021-12-24 | 秦皇岛华恒生物工程有限公司 | Beta-alanine biological enzyme synthesis method with high conversion rate and complete device thereof |
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Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103320480A (en) * | 2013-06-25 | 2013-09-25 | 南京大学 | Method for preparing beta-alanine by coupled enzymatic reaction |
| CN103525880A (en) * | 2013-10-17 | 2014-01-22 | 张家港市华昌药业有限公司 | Application of maleic anhydride waste residue in preparation of aspartic acid and method for preparing aspartic acid by using maleic anhydride waste residue |
| CN103525880B (en) * | 2013-10-17 | 2015-04-29 | 张家港市华昌药业有限公司 | Application of maleic anhydride waste residue in preparation of aspartic acid and method for preparing aspartic acid by using maleic anhydride waste residue |
| CN103898035A (en) * | 2013-12-24 | 2014-07-02 | 安徽华恒生物科技股份有限公司 | Recombinant escherichia coli strain for producing beta-alanine as well as construction method and application thereof |
| CN103898035B (en) * | 2013-12-24 | 2015-12-09 | 安徽华恒生物科技股份有限公司 | Produce the recombinant escherichia coli strain of Beta-alanine and construction process thereof and application |
| CN104195193A (en) * | 2014-09-10 | 2014-12-10 | 中国科学院天津工业生物技术研究所 | Method for preparing beta-alanine through enzymatically catalyzing hydrolysis of high-concentration beta-aminopropionitrile |
| CN104195193B (en) * | 2014-09-10 | 2019-05-10 | 中国科学院天津工业生物技术研究所 | The method that enzymatic high concentration β-aminopropionitrile hydrolysis prepares Beta-alanine |
| CN106755155A (en) * | 2016-12-07 | 2017-05-31 | 江南大学 | A kind of method for preparing beta Alanine with double enzyme series connection |
| EP3943606A4 (en) * | 2019-03-20 | 2022-12-28 | Guang an Mojia Biotechnology Co., Ltd. | Methods for preparing b-alanine, b-alanine salt and pantothenate |
| WO2020187256A1 (en) * | 2019-03-20 | 2020-09-24 | 广安摩珈生物科技有限公司 | Methods for preparing β-alanine, β-alanine salt and pantothenate |
| CN111718969A (en) * | 2019-03-20 | 2020-09-29 | 广安摩珈生物科技有限公司 | Process for producing beta-alanine |
| CN112004937A (en) * | 2019-03-20 | 2020-11-27 | 广安摩珈生物科技有限公司 | Process for the preparation of beta-alanine, beta-alanine salts and pantothenate salts |
| CN110438039A (en) * | 2019-07-19 | 2019-11-12 | 郑州大学 | Bacterial strain is used in one plant of alanine fermentation |
| CN110438039B (en) * | 2019-07-19 | 2022-01-28 | 郑州大学 | Strain for alanine fermentation |
| CN113832201A (en) * | 2020-06-24 | 2021-12-24 | 秦皇岛华恒生物工程有限公司 | Beta-alanine biological enzyme synthesis method with high conversion rate and complete device thereof |
| CN112481244B (en) * | 2020-12-08 | 2022-05-17 | 江南大学 | Aspartase mutant and coding gene, vector, recombinant bacterium and application thereof |
| CN112481244A (en) * | 2020-12-08 | 2021-03-12 | 江南大学 | Aspartase mutant and coding gene, vector, recombinant bacterium and application thereof |
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