CN102851218B - Production method of 11-O-acetylcyathatriol and 15-O-acetylcyathatriol, and special bacterial strain thereof - Google Patents
Production method of 11-O-acetylcyathatriol and 15-O-acetylcyathatriol, and special bacterial strain thereof Download PDFInfo
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Abstract
The invention discloses a method for preparation of a compound shown in formula II and/or a compound shown in formula III by microbial fermentation, and a special bacterial strain thereof. The bacterial strain is Cyathus africanus L38, which is preserved in the General microbiological center of China Committee for Culture Collection of Microorganisms and has a preservation number of CGMCC NO.6259. The method disclosed in the invention comprises the steps of: 1) inoculating the fermentation Cyathus africanus L38 into a solid medium to conduct solid fermentation culture; and 2) adding an organic solvent into the fermented system and carrying out soaking extraction so as to obtain an extracted solution, which is then subjected to pressure reduced distillation, thus obtaining a crude extract containing the compound shown in formula II and the compound shown in formula III. In the invention, Cyathus africanus L38 is utilized to prepare the compounds shown in formula II and formula III, and is cultured by a variety of conditions. The optimal obtaining period of the compounds is detected, and high yields of the compounds can be achieved. (formula II) (formula III).
Description
Technical field
The present invention relates to method and the special strain therefore thereof of the production of cyathane class diterpene compound 11-oxygen acetyl Bird's Nest alkane triol (11-O-acetylcyathatriol) and 15-oxygen acetyl Bird's Nest alkane triol (15-O-acetylcyathatriol).
Background technology
In several centuries in past, the medicine that derives from microorganism is being brought into play huge effect aspect the disease of the serious threat human healths such as treatment tumour and cause pathogeny imcrobe infection.Fungi is one of important monoid of microorganism, and its meta-bolites abundant species, structure type uniqueness make it become the micromolecular main source of natural organic active; The active substance of originated from fungus accounts for microbe-derived more than 50% (Journal of Antibiotics 2005,58,1 – 26).Derive from the microbiotic (as penicillin etc.) of fungi, disease-resistant fungal pathogens preparation, immunosuppressor and decreasing cholesterol preparation in the past decades for clinical, for mankind's resist the disease, promote longevity and made huge contribution.The strain that the sixties in last century H.J.Brodie finds is named as the bird's-nest fungus novel species of Cyathus helenae Brodie, can be good at the growth (Masters Thesis.University of Alberta, Sept.1967) of anti-bacteria; So the people such as W.A.Ayer are to its further research, find that the mycelial crude extract of this bacterium has antibiotic activity preferably, and therefrom separate the microbiotic (this compounds has 5/,6/7 three ring skeleton) obtain several called afters " cyathin ", all there is good anti-microbial activity, can anti-bacteria, growth (the Canadian Journal of Chemistry 1971 such as actinomycetes, some fungies and mankind pathogenic bacteria, 17,1401-1407).Afterwards, the people such as W.A.Ayer utilize the method that biological activity is followed the tracks of to separate again the diterpene that obtains this monoid in other Cyathus sp., and the compound quantity of the type is constantly increased.Kawagishi etc. last century the nineties separate and obtain erinacine from Hericium ernaceum; Separate and obtain scabronine and sarcodonin(Canadian Journal of Chemistry 1973,51,3842-3854 from Sarcodon scabrosus; Tetrahedron Lett.1971,13,1917-1920.), this three compounds has identical 5/,6/7 three ring skeleton with caythin, and the distribution range of such diterpene is enlarged, all significant to biosynthesizing and the activity research thereof of studying such diterpene.
Compound 11-oxygen acetyl Bird's Nest alkane triol (11-O-acetylcyathatriol) (formula II) and 15-oxygen acetyl Bird's Nest alkane triol (15-O-acetylcyathatriol) (formula III) are (Canadian Journal of Chemistry 1979 found in Cyathus earlei Lloyd at first, 57 (24), 3332-3337), but pure compound productive rate lower (be pure compound and crude extract mass ratio, in the 7.7g crude extract, separate and obtain 132mg 11-O-acetylcyathatriol and 310mg 15-O-acetylcyathatriol).Find also to have 11-O-acetylcyathatriol(Bioscience at Hericium ernaceum again afterwards, Biotechnology, and Biochemistry 2004,68 (8), 1786-1789.), pure compound productive rate the same as Cyathus earlei Lloyd lower (approximately 0.6%, from the 461mg crude extract, separate and obtain this compound of 2.8mg).
In document " Metabolites of bird ' s nest fungi.Part 11.Diterpenoid metabolites of Cyathusearlei.; Can.J.Chem.; 1979; 57; 3332-3337. ", the author has determined the structure of active compound 11-O-acetylcyathatriol and 15-O-acetylcyathatriol by methods such as nucleus magnetic resonance, and their anti-microbial activities to Staphyloccocus aureus have been tested, result shows, these two compounds have certain inhibition activity to institute's test strain.
Tumour be one of disease of well-known serious harm human health (CA-Cancer J.Clin.2011,61,69-90).In at present numerous methods for the treatment of, because chemotherapeutics can arrive each one of whole body with blood circulation, so chemotherapy (comprising immunotherapy) is being brought into play the effect that can not replace in primary carcinoma, secondary metastatic carcinoma and leukemic treatment, (Nat.Rev.Cancer 2005,5,65-72).It is single-minded that desirable chemotherapeutics should have action target spot, body internal specific target tumor tissue, and (Science 2001,293,58-59) for the characteristics such as mechanism of action is clear and definite, and the normal tissue toxic side effect is little.Due to the continuous appearance of toxicity, side effect and the resistance of traditional chemotherapeutics, make the anti-tumor chemotherapeutic curative effect reduce or curative effect lost efficacy, therefore, find new type antineoplastic medicine, extremely urgent.The focus of antitumor drug research and development is at present mainly transferred to the new type antineoplastic medicine for the tumor signal Signal Transduction Pathways from traditional cytotoxic drug.
In patent literature, all do not relate to the anti-tumor activity report of 11-O-acetylcyathatriol and 15-O-acetylcyathatriol compound at home and abroad.
Summary of the invention
The purpose of this invention is to provide a kind of microorganism fermentation preparation formula II(11-O-acetylcyathatriol that utilizes) and/or formula III (15-O-acetylcyathatriol) shown in method and the special strain therefore thereof of compound.
(formula II) (formula III)
Special strain therefore provided by the present invention is C.africanus (Cyathus africanus) L38, and this strains separation is from Wutai, Shanxi Province, and the basidioma of L38 bacterial strain is back taper, high 6-8mm, the wide 9-11*6.5-8 μ of oral area m; Coated without obvious vertical stripe; Parcel tool individual layer cortex and film, oblate, 1.9-2.5*1.8-2.0mm; Sporidium is avette has a little point, 9-12.0*6.5-8.0.
C.africanus (Cyathus africanus) L38 is on 07 04th, 2012, be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center and (be called for short CGMCC, address is: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica), deposit number is CGMCC NO.6259.
The application of fermentation C.africanus (Cyathus africanus) L38 CGMCC NO.6259 in compound (15-O-acetylcyathatriol) shown in compound shown in preparation formula II (11-O-acetylcyathatriol) and/or formula III also belongs to protection scope of the present invention.
The present invention also provides the method for utilizing compound (15-O-acetylcyathatriol) shown in compound (11-O-acetylcyathatriol) shown in fermentation C.africanus (Cyathus africanus) L38 CGMCC NO.6259 preparation formula II and formula III.
The method of compound (15-O-acetylcyathatriol) shown in compound shown in described preparation formula II (11-O-acetylcyathatriol) and formula III comprises the following steps:
1) C.africanus (Cyathus africanus) the L38 CGMCC NO.6259 that will ferment is inoculated on solid medium and carries out the solid fermentation cultivation;
2) after the solid fermentation of step 1), to substratum, add organic solvent extraction, obtain extracting solution, by described extracting solution underpressure distillation, obtain crude extract;
Wherein, in step 1), solid medium is mixed to get by base material and water; Optional one or more arbitrary combination in wheat, polished rice, corn, glutinous rice, long-grained nonglutinous rice and the seed of Job's tears of described base material; The water content of described solid medium is the 100-150% of described base material gross weight.
In described step 1), the condition that described solid fermentation is cultivated is 18-28 ℃, the static cultivation of lucifuge 20-60 days.The condition optimization that described solid fermentation is cultivated is 25 ℃, static cultivation 30 days.
Described step 2), in, described organic solvent is any one in ethyl acetate, acetone, methyl alcohol, ethanol, chloroform, Virahol and propyl carbinol.
In described method, also comprise and described crude extract is carried out to the normal pressure silica gel column chromatography or gel chromatography separation obtains compound (15-O-acetylcyathatriol) shown in compound shown in formula II (11-O-acetylcyathatriol) and/or formula III.
Wherein, the method of normal pressure silica gel column chromatography, the mixed solution of chloroform and acetone of take is solvent, the gradient that is 100:0,100:1,50:1,30:1,20:1,10:1,5:1,3:1,2:1 successively according to the ratio of chloroform in solvent and acetone is carried out gradient elution, 1500 milliliters of each gradient elution solvents, every 350 milliliters are collected as a stream part; After second the stream part concentrating under reduced pressure drying of the eluting solvent wash-out that is 10:1 by the ratio of chloroform and acetone, be dissolved in 10 ml methanol solution, room temperature is standing, separates out colourless crystallization, obtains the compound shown in the formula II; After the 3rd the stream part concentrating under reduced pressure drying of the eluting solvent wash-out that is 10:1 by the ratio of chloroform and acetone, be dissolved in 10 ml methanol solution, the room temperature standing over night, separate out colourless crystallization, obtains the compound shown in the formula III.Described normal pressure silica gel column chromatography (silica gel column chromatography separation) filler used is 200-300 purpose silica gel particle, and filler is 150g, and the silicagel column specification is Φ 4.5 * 80cm.
In described step 1), also be included in before solid fermentation C.africanus (Cyathus africanus) L38CGMCC NO.6259 is carried out to preculture on the PDA solid medium, get mycelium after preculture and be transferred in liquid nutrient medium and cultivated; Pre-incubated condition is: 25-28 ℃, and lucifuge is cultivated 5-7 days; The condition of liquid culture is: 25-28 ℃, lucifuge is cultivated 7-10 days.
Consisting of of described PDA substratum: potato 200g, glucose 20g, agar powder 16g, water is settled to 1L; Consisting of of described liquid nutrient medium: glucose 4.0g/L, malt extract 10.0g/L, yeast 4.0g/L, water is settled to 1L.
The present invention utilizes superior strain C.africanus (Cyathus africanus) L38 fermentation to prepare compound 11-O-acetylcyathatriol and 15-O-acetylcyathatriol, and adopt multiple condition to be cultivated to this bacterium, detect the best of this compound and obtained period, all can obtain higher pure compound productive rate (be pure compound and crude extract mass ratio, wherein compound 11-O-acetylcyathatriol is generally more than 12%).Can realize the optimal yield of compound, and then have a good application prospect.
Embodiment
Below with embodiment, be described in further detail the present invention, but content of the present invention is not limited to this.
Experimental technique described in following embodiment, if no special instructions, be ordinary method; Described reagent and biomaterial, if no special instructions, all can obtain from commercial channels.
Embodiment 1, the acquisition of producing bacterial strain C.africanus (Cyathus africanus) L38 of cyathane class diterpene compound 11-O-acetylcyathatriol and 15-O-acetylcyathatriol
One, the separation of C.africanus (Cyathus africanus) L38
The inventor is on the high flux screening basis of Secondary Metabolites of Microorganisms active compound, and the extract that separates the solid fermentation of the strain C.africanus (Cyathus africanus) obtained from Wutai, Shanxi Province Cyathus africanus sporophore has anti-tumor activity preferably; By the further separation and purification under active tracking instruction, obtain output higher and easily be purified to the bacterial strain of compound, and by this bacterial strain called after L38.
Specifically separation method is, at first, with ethanol for disinfection, the sporophore gathered is carried out to surface sterilization, then uses sterile water wash, then, parcel in sporophore is cut into small pieces, last, is seeded on the PDA substratum, and separation and purification obtains bacterial strain.
Two, the evaluation of C.africanus (Cyathus africanus) L38.
1, physiology and morphology biochemical identification
The basidioma of L38 bacterial strain is back taper, high 6-8mm, and the wide 5-8mm of oral area, the mycelia pad of base portion is obvious, diameter 5.5mm; Coated outside light color, inboard light brown, nothing is vertical stripe obviously, and peristoma is smooth without tasselled; Parcel tool individual layer cortex and film, oblate, 1.9-2.5*1.8-2.0mm; Sporidium, avette, there is a little point, 9-12.0*6.5-8.0 μ m.
This L38 bacterial strain meets the feature that following C.africanus (Cyathus africanus) pattern DAOM200370 describes:
C.africanus (Cyathus africanus) belongs to Genus Cyathus (Cyathus), and its Main Morphology feature is that basidioma is cup-shaped, turbination or doline, high 5-8mm, and the wide 5-8mm of oral area, diameter can reach 6mm, base portion tool mycelia pad; Be coated with three layers (middle for paraplectenchyma), outside sandy soil look, dirty brown color, olive colour, light brown to brown, by having, powder is yellow, yellowish, the thin wool of sandy soil look, brown color, often is close to coated wall, sometimes also forms tuftlet, smoothly without striped; Inboard is generally slightly more shallow than the outside, and most level and smooth, peristoma is smooth.A white thin epiphragma is stamped at top, when ripe, generally disappears.Parcel is flat, wide ellipse, and 2-2.5*1.8-2.3mm, grey or black, tool individual layer cortex, one deck is pale yellow to beige film.Sporidium is avette, the little point of a most end tool, and 8.5-11.0 (14.0) * (5.5-) 6.5-8.0 (9.0) μ m wall is thinner, colourless, non-starchiness, general wall thickness; Hyphae colorless, have obvious clamp connexion; Give birth on ground, rotten wood is given birth to, excrement is given birth to or is born on litter layer.
Molecular Identification to the L38 bacterial strain shows, the sequence of its 18Sr DNA is as shown in sequence in sequence table 1, with the sequence similarity degree that in American National biotechnology information center (NCBI), accession number is the DQ463342.1 C.africanus, reach 100%, according to above morphology, physiological and biochemical property and 18Sr DNA, bacterial strain L38 is accredited as to C.africanus (Cyathus africanus).
C.africanus (Cyathus africanus) L38 is on 07 04th, 2012, be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center and (be called for short CGMCC, address is: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica), deposit number is CGMCC NO.6259.
Embodiment 2, utilize C.africanus (Cyathus africanus) L38 to produce 11-O-acetylcyathatriol and 15-O-acetylcyathatriol
One, bacterial strain activation, fermentation culture
1, the acquisition of seed liquor:
C.africanus (Cyathus africanus) L38 is inoculated on PDA test tube nutrient agar inclined-plane, carries out preculture; Pre-incubated condition is: 25 ℃, lucifuge is cultivated 7 days (the PDA substratum forms: potato 200g, glucose 20g, agar powder 16g, pure water is settled to 1L (pH nature)).After mycelia is covered with whole inclined-plane, cultivated under aseptic condition, mycelium being transferred to liquid nutrient medium, obtain seed culture fluid; The condition of liquid culture is: 25 ℃, lucifuge is cultivated 7 days.Liquid nutrient medium: glucose 4.0g/L, malt extract (Malt Extract) 10.0g/L, yeast (Yeast Extract) 4.0g/L, water is settled to 1L; Yeast extract (Yeast Extract) is purchased from Oxoid Ltd., and lot number is 1074139.
2, solid fermentation is cultivated:
Get 10 milliliters of seed culture fluids and be inoculated into respectively through being equipped with in solid medium 500 milliliters of triangular flasks of (solid medium is comprised of 80g long-grained nonglutinous rice and 120ml water) of sterilizing, inoculate 10 bottles, 25 ℃, lucifuge fermentation culture 30 days.(mycelium dry weight after each triangular flask fermentation is 75.5g).
Two, the extraction of compound separates
1, organic solvent extraction
Solid fermentation is added to 300 milliliters of ethyl acetate respectively in each triangular flask after finishing, and soak at room temperature extracts one week, repeat to extract 3 times, combined ethyl acetate extracting solution vacuum distillation drying, obtain medicinal extract, i.e. 12.5g ethyl acetate extract (extraction yield is 12.5/755=1.66%).
2, the separation and purification of compound
Ethyl acetate extract is opened to column chromatography (200-300 of Qingdao Marine Chemical Co., Ltd. order, column chromatography silica gel 150g through silica gel; Φ 4.5 * 80cm) separate, chloroform-acetone mixed solvent gradient is washed (100:0,100:1,50:1,30:1,20:1,10:1,5:1,3:1,2:1; Volume ratio), 1500 milliliters of each gradient elution solvents, every 350 milliliters are collected as a stream part.To after second of chloroform-acetone 10:1 wash-out stream part concentrating under reduced pressure drying, be dissolved in 10 ml methanol solution, the room temperature standing over night, separate out colourless crystallization, obtain compound shown in the formula II (11-O-acetylcyathatriol, 2.1g(pure compound productive rate is 2.1/12.5=16.8%)).To after the 3rd of chloroform-acetone 10:1 wash-out stream part concentrating under reduced pressure drying, be dissolved in 10 ml methanol solution, the room temperature standing over night, separate out colourless crystallization, obtains the compound (15-O-acetylcyathatriol shown in the formula III, 0.32g the pure compound productive rate is 0.32/12.5=2.56%).
3, compound structure conclusive evidence
Formula II compound: clear crystal; The specific rotatory power value
hRESIMS m/z385.2357[M+Na]
+; The hydrogen spectrum (
1h-NMR) and carbon spectrum (
13c-NMR) in Table 1.According to the physico-chemical property of compound, hydrogen spectrum, carbon spectrum data, and with the reference numeric ratio to (Can.J.Chem., 1979,57 (24), 3332-3337; Biosci.Biotechnol.Biochem., 2004,68 (8), 1786-1789.), determine that the structure of this compound is identical with 11-O-acetylcyathatriol.
Formula III compound: colorless oil; The specific rotatory power value
hRESIMS m/z385.2340[M+Na]
+; The hydrogen spectrum (
1h-NMR) in Table 1.According to the physico-chemical property of compound, hydrogen spectrum data, and with the reference numeric ratio to (Can.J.Chem., 1979,57 (24), 3332-3337), determine that the structure of this compound is identical with 15-O-acetylcyathatriol.
The NMR data of table 1. compound
a?
1H?NMR?at?500Hz,
13C?NMR?at?125Hz?in?CD
3OD.
b?
1H?NMR?at?500Hz?in?CDCl
3.
Embodiment 3, utilize C.africanus (Cyathus africanus) L38 to produce 11-O-acetylcyathatriol and 15-O-acetylcyathatriol
Embodiment 3 methods are with embodiment 2, difference: one, and the solid culture based formulas, its formula is: the 80g seed of Job's tears, 120ml distilled water; Two, incubation time is also different, is 40 days; Three, the mycelium dry weight after each triangular flask fermentation is 74.3g; Four, in organic solvent extraction, solvent for use is acetone, obtains 13.2g extract (extraction yield is 13.2/743=1.77%).Separate and obtain compound (11-O-acetylcyathatriol shown in the formula II according to the method for the step 2 of embodiment 2,1.7g(the pure compound productive rate is 12.9%)), compound shown in the formula III (the pure compound productive rate is 2.35% for 15-O-acetylcyathatriol, 0.31g)
Embodiment 4, utilize C.africanus (Cyathus africanus) L38 to produce 11-O-acetylcyathatriol and 15-O-acetylcyathatriol
Embodiment 4 methods are with embodiment 2, difference: one, and the solid culture based formulas, its formula is: corn 40g, wheat 40g, 120ml distilled water; Two, the mycelium dry weight after each triangular flask fermentation is 75.7g; Three, in organic solvent extraction, solvent for use is methyl alcohol, obtains 15.0g extract (extraction yield is 15.0/757=1.98%).Compound shown in the formula II (11-O-acetylcyathatriol, 1.83g(pure compound productive rate is 12.2%)), the compound shown in the formula III (the pure compound productive rate is 1.87% for 15-O-acetylcyathatriol, 0.28g)
Embodiment 5, utilize C.africanus (Cyathus africanus) L38 to produce 11-O-acetylcyathatriol and 15-O-acetylcyathatriol
Embodiment 5 methods are with embodiment 2, difference: one, and the solid culture based formulas, its formula is: corn 80g, 100ml distilled water; Two, incubation time is also different, is 40 days; Three, the mycelium dry weight after each triangular flask fermentation is 76.4g; Four, in organic solvent extraction, solvent for use is ethanol, obtains 17.7g extract (extraction yield is 17.7/764=2.32%).Compound shown in the formula II (11-O-acetylcyathatriol, 2.2g(pure compound productive rate is 12.4%)), the compound shown in the formula III (the pure compound productive rate is 2.45% for 15-O-acetylcyathatriol, 0.44g)
Claims (4)
1. the method for compound shown in compound shown in a preparation formula II and formula III comprises the following steps:
1) C.africanus (Cyathus africanus) L38CGMCC NO.6259 is inoculated on solid medium and carries out the solid fermentation cultivation;
2) in the system after the step 1) fermentation, add organic solvent to soak and extract the extracting solution that obtains containing compound shown in compound shown in formula II and formula III, by described extracting solution underpressure distillation, obtain containing the crude extract of compound shown in compound shown in formula II and formula III;
Wherein, in step 1), described solid medium is mixed to get by base material and water, and described base material is selected from one or more arbitrary combination in wheat, polished rice, corn, glutinous rice, Gorgon fruit, long-grained nonglutinous rice and the seed of Job's tears; The water content of described solid medium is the 100-150% of described base material gross weight;
In described step 1), the culture condition that described solid fermentation is cultivated is 18-28 ℃, the static cultivation of lucifuge 20-60 days;
In described method, also comprising step 2) crude extract that obtains separates with silica gel column chromatography, the mixed solution of chloroform and acetone of take is solvent, the gradient that is 100:0,100:1,50:1,30:1,20:1,10:1,5:1,3:1,2:1 successively according to the ratio of chloroform in solvent and acetone is carried out gradient elution, 1500 milliliters of each gradient elution solvents, every 350 milliliters are collected as a stream part; After second the stream part concentrating under reduced pressure drying of the eluting solvent wash-out that is 10:1 by the ratio of chloroform and acetone, be dissolved in 10 ml methanol solution, room temperature is standing, separates out colourless crystallization, obtains the compound shown in the formula II; After the 3rd the stream part concentrating under reduced pressure drying of the eluting solvent wash-out that is 10:1 by the ratio of chloroform and acetone, be dissolved in 10 ml methanol solution, the room temperature standing over night, separate out colourless crystallization, obtains the compound shown in the formula III;
2. method according to claim 1 is characterized in that: in described step 1), the culture condition that described solid fermentation is cultivated is 25 ℃, the static cultivation of lucifuge 30 days.
3. method according to claim 1 and 2, it is characterized in that: described step 2), described organic solvent is any one in ethyl acetate, acetone, methyl alcohol, ethanol, chloroform, Virahol and propyl carbinol.
4. method according to claim 1, it is characterized in that: in described step 1), also be included in before solid fermentation C.africanus (Cyathus africanus) L38CGMCC NO.6259 is carried out to preculture on the PDA solid medium, get mycelium after preculture and be transferred in liquid nutrient medium and cultivated; Pre-incubated condition is: 25-28 ℃, and lucifuge is cultivated 5-7 days; The condition of liquid culture is: 25-28 ℃, and lucifuge is cultivated 7-10 days;
Consisting of of described PDA substratum: potato 200g, glucose 20g, agar powder 16g, water is settled to 1L; Consisting of of described liquid nutrient medium: glucose 4.0g, malt extract 10.0g, yeast extract 4.0g, water is settled to 1L.
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