CN102850464A - Method for extracting and purifying Chinese yam polysaccharide - Google Patents
Method for extracting and purifying Chinese yam polysaccharide Download PDFInfo
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- CN102850464A CN102850464A CN2012103827695A CN201210382769A CN102850464A CN 102850464 A CN102850464 A CN 102850464A CN 2012103827695 A CN2012103827695 A CN 2012103827695A CN 201210382769 A CN201210382769 A CN 201210382769A CN 102850464 A CN102850464 A CN 102850464A
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- chinese yam
- yam polysaccharide
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- water extraction
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Abstract
The invention relates to a method for extracting and purifying Chinese yam polysaccharide. The method comprises the steps of: drying peeled Chinese yam, and carrying out ultrasonic cell disintegration; then, extracting under the conditions that the water extraction temperature is 40 DEG C, the ultrasound time is 50 minutes, the water extraction time is 30 minutes and the material-liquid ratio is 1: 16, centrifuging, and carrying out vacuum concentration on supernate; precipitating with using 95% ethanol the volume of which is three times of volume of the supernate, and redissolving precipitate; carrying out gradient elution on cellulose diethylaminoethyl (DEAE)-52 ion exchange column by NaCl solution; tracking and detecting by using a phenol-sulfuric acid method; collecting and merging peak pipe eluent; and finally, freezing and drying to obtain the signal Chinese yam polysaccharide component.
Description
Technical field: the extracting and purifying method that the invention provides a kind of Chinese yam polysaccharide.
Background technology: Chinese yam is the plant of dietotherapeutic, the mineral element that contains the nutritive ingredients such as robust fibre, pectin, glycoprotein, amylase, phlegmatic temperament, saponin(e, wallantoin, batatasins, polysaccharide and activeconstituents and the needed by human body such as calcium, phosphorus, iron and iodine has the tonifying spleen nourishing the stomach, an effect of the beneficial lung that promotes the production of body fluid, antidiarrheal dampness removing.Chinese yam polysaccharide has the effects such as adjusting is immune, antitumor, adjusting blood sugar, it forms more complicated, Chinese scholars has been done a large amount of work at aspects such as the extraction of Chinese yam polysaccharide, separation, purifying, so that the nutritive value of Chinese yam, pharmaceutical use and disease-resistant mechanism etc. gradually are found and confirm.
Summary of the invention: the extracting and purifying method that the objective of the invention is to provide a kind of Chinese yam polysaccharide.
The present invention is achieved in that the Chinese yam that takes by weighing the dry peeling of 20g, supersonic cell is pulverized, extract with 40 ℃ of water extraction temperature, ultrasonic time 50min, water extraction time 30min, 1: 16 condition of solid-liquid ratio, centrifugal, the supernatant liquor vacuum concentration that obtains, with the 95% ethanol precipitation of 3 times of volumes, throw out redissolves.Cellulose DEAE-52 ion exchange column on the concentrated solution, NaCL solution gradient wash-out, the phenolsulfuric acid method is followed the tracks of and is detected, and obtains single polysaccharide fraction.
Embodiment: the Chinese yam that takes by weighing the dry peeling of 20g, supersonic cell porphyrization time 2s, intermittent time 3s, net cycle time 2min, extract with 40 ℃ of water extraction temperature, ultrasonic time 50min, water extraction time 30min, 1: 16 condition of solid-liquid ratio, centrifugal, the supernatant liquor vacuum concentration, with the 95% ethanol precipitation of 3 times of volumes, throw out redissolves.Get the concentrated crude extract of 10mL, inject Cellulose DEAE-52 ion exchange column, use successively 0.1mol/L, 0.5mol/L, each 200mL gradient elution of 1mol/LNaCL solution, flow velocity 1mL/min, collect elutriant, every pipe is collected 6mL, collects the liquid pipe and follows the tracks of detection with the phenolsulfuric acid method, and the 490nm place measures absorbancy, draw elution curve, select optimal absorption peak Fractional Collections, lyophilize obtains single polysaccharide fraction.
Claims (3)
1. the extracting method of a Chinese yam polysaccharide, it is characterized in that: the Chinese yam that takes by weighing the dry peeling of 20g, supersonic cell porphyrization time 2s, intermittent time 3s, net cycle time 2min bathes extraction at the different treatment Water Under, 5000rpm/min is centrifugal, the supernatant liquor vacuum concentration that obtains, concentrated solution is with the 95% ethanol precipitation of 3 times of volumes, and throw out redissolves.
2. the extracting method of a kind of Chinese yam polysaccharide according to claim 1 is characterized in that the optimal conditions that extracts is: 40 ℃ of water extraction temperature, ultrasonic time 50min, water extraction time 30min, solid-liquid ratio 1: 16.
3. the purification process of a Chinese yam polysaccharide, it is characterized in that: according to claim 1 with 2 described extracting method, obtain concentrated sample liquid 10mL, inject Cellulose DEAE-52 ion exchange column, use successively 0.1mol/L, 0.5mol/L, each 200mL gradient elution of 1mol/LNaCL solution, flow velocity 1mL/min, collect elutriant, every pipe is collected 6mL, collects the liquid pipe and follows the tracks of color developing detection with the phenolsulfuric acid method, and the 490nm place measures absorbancy, draw elution curve, select optimal absorption peak Fractional Collections, lyophilize obtains single polysaccharide fraction.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012103827695A CN102850464A (en) | 2012-10-11 | 2012-10-11 | Method for extracting and purifying Chinese yam polysaccharide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012103827695A CN102850464A (en) | 2012-10-11 | 2012-10-11 | Method for extracting and purifying Chinese yam polysaccharide |
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| CN102850464A true CN102850464A (en) | 2013-01-02 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2012103827695A Pending CN102850464A (en) | 2012-10-11 | 2012-10-11 | Method for extracting and purifying Chinese yam polysaccharide |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103720599A (en) * | 2014-01-03 | 2014-04-16 | 湖北省农业科学院农产品加工与核农技术研究所 | Method for preparing moisturizing agent from yam skin residues |
| CN104260990A (en) * | 2014-08-06 | 2015-01-07 | 宁波华丰包装有限公司 | Medical instrument packaging film and manufacturing method thereof |
| CN106905381A (en) * | 2017-03-11 | 2017-06-30 | 顺德职业技术学院 | The efficient concentrating type chemical modification synthetic method of natural products medicine |
| CN111620957A (en) * | 2020-04-10 | 2020-09-04 | 中国科学院上海药物研究所 | Chinese yam polysaccharide, preparation method and application thereof |
| CN111875716A (en) * | 2020-09-11 | 2020-11-03 | 淄博职业学院 | Method for extracting and purifying high-antioxidant-activity Chinese yam polysaccharide |
-
2012
- 2012-10-11 CN CN2012103827695A patent/CN102850464A/en active Pending
Non-Patent Citations (1)
| Title |
|---|
| 李金忠，马海乐，吴沿友: "超声波提取山药多糖的研究", 《粮油食品科技》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103720599A (en) * | 2014-01-03 | 2014-04-16 | 湖北省农业科学院农产品加工与核农技术研究所 | Method for preparing moisturizing agent from yam skin residues |
| CN103720599B (en) * | 2014-01-03 | 2015-09-16 | 湖北省农业科学院农产品加工与核农技术研究所 | A kind of method preparing wetting agent from yam skin slag |
| CN104260990A (en) * | 2014-08-06 | 2015-01-07 | 宁波华丰包装有限公司 | Medical instrument packaging film and manufacturing method thereof |
| CN104260990B (en) * | 2014-08-06 | 2016-12-07 | 宁波华丰包装有限公司 | A kind of medical device package film and manufacture method thereof |
| CN106905381A (en) * | 2017-03-11 | 2017-06-30 | 顺德职业技术学院 | The efficient concentrating type chemical modification synthetic method of natural products medicine |
| CN111620957A (en) * | 2020-04-10 | 2020-09-04 | 中国科学院上海药物研究所 | Chinese yam polysaccharide, preparation method and application thereof |
| CN111620957B (en) * | 2020-04-10 | 2021-12-10 | 中国科学院上海药物研究所 | Chinese yam polysaccharide, preparation method and application thereof |
| CN111875716A (en) * | 2020-09-11 | 2020-11-03 | 淄博职业学院 | Method for extracting and purifying high-antioxidant-activity Chinese yam polysaccharide |
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Application publication date: 20130102 |