CN102846675A - New use of Cordyceps sobolifera - Google Patents
New use of Cordyceps sobolifera Download PDFInfo
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- CN102846675A CN102846675A CN2011101782750A CN201110178275A CN102846675A CN 102846675 A CN102846675 A CN 102846675A CN 2011101782750 A CN2011101782750 A CN 2011101782750A CN 201110178275 A CN201110178275 A CN 201110178275A CN 102846675 A CN102846675 A CN 102846675A
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- periostracum cicadae
- cicadae
- sporophore
- periostracum
- cordyceps sobolifera
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Abstract
The invention relates to a new use of Cordyceps sobolifera, especially a use of Cordyceps sobolifera or its fruiting body in preparing medicament proliferating osteocyte. The invention opens a new direction in the research for application of Cordyceps sobolifera, and brings gospel for patients with osteoporosis.
Description
Technical field
The present invention relates to biological technical field, be specifically related to the new purposes of Periostracum cicadae.
Background technology
Osteoporosis be a kind of common with increase closely-related metabolic osteopathy in age, modern medicine is thought the generation of osteoporosis and osteoblast, and (couple unbalance of (osteoblast, OB) and osteoclast is relevant.The effect that OB has is synthetic, secretion forms bone matrix collagen and glycoprotein, in that to keep organismic internal environment stable, physiological mechanism regulate and bone metabolism disease in bring into play Main Function.Promote that osteoblastic proliferation is the main path of Chinese herb prevention osteoporosis, osteoblast cultured in vitro is the important research means of evaluating drug effect and new drug development.
Chinese medicine Periostracum cicadae (Cordycepes cicadea), it is a kind of and the similar medicinal entomogenous fungi of Cordyceps, colonize in the bacterium worm complex that forms on some Cicadae nymph for Paecilomyces cicadidae(Miquel)Samson (Paecilomyces cicadae), be China's tradition rare Chinese medicine, understanding and the existing long history of utilization in China.Doctor family thinks that Periostracum cicadae is a kind of valuable ingredient of Chinese medicine close with Cordyceps.Modern study shows that Periostracum cicadae has many-sided pharmacological action, can the enhancing human body immunity function, and nervous system regulation, antipyretic-antalgic improves renal function, regulates lipid metabolism, resisting fatigue, anti-stress, antitumor action etc.But no matter be natural Periostracum cicadae or the Periostracum cicadae of artificial culture, all do not have it to treat osteoporotic research and report.
Summary of the invention
The purposes of sporophore in preparation osteocyte propagation medicine that the purpose of this invention is to provide Periostracum cicadae (Cordycepes cicadea) or Periostracum cicadae.
Further, described osteocyte propagation medicine is the medicine for the treatment of osteoporosis.
Described Periostracum cicadae can be natural Periostracum cicadae or artificial Periostracum cicadae.
Natural Periostracum cicadae also claims Cicadae bacterium Cordyceps sobolifera, is a kind of marvellous biology with animal and plant feature, and root is the larva body of Cicadae pupa or mountain Cicadae, and flower is from single or 2-3 Cicadae larva head grows out, and about one-inch how long, the branch of blooming from the top.According to relevant researching and proposing, Periostracum cicadae belongs to biological ill phenomenon, is the interdependent assembly of a kind of entomogenous fungi.Arrive autumn, Cicadae pierces in the soil, becomes gradually the Cicadae pupa, and is parasitic by the Cordyceps mushroom before emergence, when life spare part is suitable, just begins to sprout into mycelium, absorbs the nutrition of polypide, and final polypide is occupied fully by mycelium and only remains the next body.In all things on earth recovery time, mycelium is converted into the perfect stage from the vegetative phase again, and the branch of gradually blooming from the top is so the Periostracum cicadae of gaining the name.
Artificial Periostracum cicadae is the tunning of Paecilomyces cicadidae(Miquel)Samson through fermenting and obtaining that will separate from natural Periostracum cicadae, and comprises mycoplasma and sporophore, and composition and effect and natural Periostracum cicadae are approximate.
The sporophore of Periostracum cicadae of the present invention or Periostracum cicadae refers to think complete Periostracum cicadae, can only be the sporophore of Periostracum cicadae also.
The purposes of sporophore in pharmacy of indication Periostracum cicadae of the present invention or Periostracum cicadae, both the sporophore of Periostracum cicadae or Periostracum cicadae directly can be used as medicine, also the sporophore of Periostracum cicadae or Periostracum cicadae can be adopted conventional method effective component extracting after (such as the decoction of routine, decoction and alcohol sedimentation technique, ethanol extract from water precipitation, salting out method etc.) be used as medicine.
The present invention finds after deliberation, and Periostracum cicadae or its sporophore have the effect of propagation to osteocyte, and this experiment means that Periostracum cicadae can be used to treat osteoporosis.The dosage that Periostracum cicadae is used for the treatment of osteoporosis can be 50mg-100mg/kg, and the present invention is that new approach has been opened up in the application of Periostracum cicadae.
The specific embodiment
Embodiment 1 Periostracum cicadae and Periostracum cicadae sporophore are to the effect of MG63 osteocyte propagation
1, experiment material and method
1.1 experiment material
Vitro culture of human osteocyte MG63 (Chinese cell bank classical collection cell centre); DMEM culture medium (Gibco, the U.S.); Hyclone (Tianjin Hao ocean biological product); Trypsin Amresco, the U.S.); Dexamethasone, β one sodium glycerophosphate, phosphorylation ascorbic acid (ASAP), Thiazolyl blue (Mrrr), trypsin, II Collagenase Type, a naphthylphosphoric acid (Sigma company); Periostracum cicadae (natural Periostracum cicadae) and Periostracum cicadae sporophore (for the Periostracum cicadae of artificial culture) (Shanghai Pan Asia life science institute provides).
Extract with suitable quantity of water before the experiment, filter, be condensed into the concentration of 1g/ml, dilute before the use.
1.2 method
1.2.1MG63 the cultivation of cell: from liquid nitrogen, take out frozen MG63 cell, put rapidly in 37 ℃ of warm water of people, shake gently cryopreservation tube and make its content melt as early as possible.To centrifuge tube, add fresh culture 10mL with suction pipe sucking-off cell suspension, the featheriness suspension cell, the centrifugal 5min of 1000r/min removes supernatant, repeats to wash 1 time with culture fluid again.The MG63 cell is inoculated in the DMEM culture medium that contains 10% hyclone, 100U/mL penicillin, 100Ixg/mL streptomycin with 5 * 10/mL density, places to contain 5%C02,37 ℃, the CO2 gas incubator of saturated humidity and cultivate, change liquid behind the 24h 1 time.Good when cell growth state, when covering at the bottom of the culture bottle 80%, with 0.25% trypsinization, go down to posterity.
1.2.2 the mensuration of cell proliferation: osteoblast is inoculated in 96 orifice plates by 5 * 10, every hole inoculation 0.2mL.Behind cell attachment, inhale and to abandon culture fluid, add respectively the 100mL final concentration and be 0.1,0.3, the Periostracum cicadae water extract of 1mg/mL, Periostracum cicadae sporophore water extract, negative controls adds isopyknic DMEM culture medium, continues to cultivate 48h.The sucking-off culture fluid is measured the cell proliferation situation with mtt assay, and enzyme-linked immunosorbent assay instrument is measured the absorbance (OD) in each hole, and the mensuration wavelength is 570nm.
1.2.3 statistical procedures is used the SPSS11.0 software kit experimental data is carried out statistical analysis, relatively adopt one factor analysis of variance, experimental group and matched group relatively to adopt the Dunnett-t check between group.
2 results: see the following form
Periostracum cicadae is on the impact of osteoblastic proliferation
※: variable concentrations Periostracum cicadae group and the comparison p that contrasts＜0.05
The result shows that along with the increase of dosage, each organizes cell appearance propagation in various degree, and is obvious with natural Periostracum cicadae and Periostracum cicadae sporophore high concentration group propagation, compares with the matched group that does not add medicine, and significant difference (P＜0.05) is arranged.Conclusion: Periostracum cicadae and Periostracum cicadae sporophore all have certain proliferation function to the human osteoblast cell, can be used for osteoporotic treatment.
Claims (3)
1. the purposes of the sporophore of Periostracum cicadae or Periostracum cicadae in preparation osteocyte propagation medicine.
2. the purposes of the sporophore of Periostracum cicadae or Periostracum cicadae as claimed in claim 1 is characterized in that, described osteocyte propagation medicine is the medicine for the treatment of osteoporosis.
3. the purposes of the sporophore of Periostracum cicadae or Periostracum cicadae as claimed in claim 1 is characterized in that, described Periostracum cicadae is natural Periostracum cicadae or artificial Periostracum cicadae.
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| CN201110178275.0A CN102846675B (en) | 2011-06-28 | 2011-06-28 | New use of Cordyceps sobolifera |
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| CN201110178275.0A CN102846675B (en) | 2011-06-28 | 2011-06-28 | New use of Cordyceps sobolifera |
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| CN102846675A true CN102846675A (en) | 2013-01-02 |
| CN102846675B CN102846675B (en) | 2014-09-10 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105708868A (en) * | 2014-12-05 | 2016-06-29 | 葡萄王生技股份有限公司 | Cordyceps cicadae/cordyceps sobolifera mycelium active substance and composition for protecting nerve cells |
| CN106265768A (en) * | 2015-06-04 | 2017-01-04 | 葡萄王生技股份有限公司 | Periostracum cicadae active substance, its preparation method, the medical composition comprising it and application thereof |
Citations (1)
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| CN1541704A (en) * | 2003-04-17 | 2004-11-03 | ӡ�����Ƽ��ɷ�����˾ | Osteoprosis treating medicament and osteoclast creating inhibitor |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1541704A (en) * | 2003-04-17 | 2004-11-03 | ӡ�����Ƽ��ɷ�����˾ | Osteoprosis treating medicament and osteoclast creating inhibitor |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105708868A (en) * | 2014-12-05 | 2016-06-29 | 葡萄王生技股份有限公司 | Cordyceps cicadae/cordyceps sobolifera mycelium active substance and composition for protecting nerve cells |
| CN105708868B (en) * | 2014-12-05 | 2020-04-10 | 葡萄王生技股份有限公司 | Cordyceps cicadae mycelium active substance and composition for protecting nerve cells |
| CN106265768A (en) * | 2015-06-04 | 2017-01-04 | 葡萄王生技股份有限公司 | Periostracum cicadae active substance, its preparation method, the medical composition comprising it and application thereof |
| CN106265768B (en) * | 2015-06-04 | 2019-12-31 | 葡萄王生技股份有限公司 | Cordyceps cicadae active substance, preparation method thereof, pharmaceutical composition containing cordyceps cicadae active substance and application of cordyceps cicadae active substance |
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| CN102846675B (en) | 2014-09-10 |
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