CN102845310A - Method for preparing monstera deliciosa plant tissue medium - Google Patents
Method for preparing monstera deliciosa plant tissue medium Download PDFInfo
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- CN102845310A CN102845310A CN2012103838030A CN201210383803A CN102845310A CN 102845310 A CN102845310 A CN 102845310A CN 2012103838030 A CN2012103838030 A CN 2012103838030A CN 201210383803 A CN201210383803 A CN 201210383803A CN 102845310 A CN102845310 A CN 102845310A
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- China
- Prior art keywords
- medium
- rooting
- litre
- liter
- monstera deliciosa
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- 240000000987 Monstera deliciosa Species 0.000 title claims abstract description 21
- 238000000034 method Methods 0.000 title claims abstract description 7
- 239000002609 medium Substances 0.000 claims abstract description 18
- 235000002790 Monstera deliciosa Nutrition 0.000 claims abstract description 16
- 239000006160 differential media Substances 0.000 claims abstract description 16
- 229920001817 Agar Polymers 0.000 claims abstract description 14
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 14
- 229930006000 Sucrose Natural products 0.000 claims abstract description 14
- 239000008272 agar Substances 0.000 claims abstract description 14
- 239000000843 powder Substances 0.000 claims abstract description 14
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 11
- 239000012882 rooting medium Substances 0.000 claims abstract description 10
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 claims abstract description 9
- 238000004161 plant tissue culture Methods 0.000 claims abstract description 6
- 239000005556 hormone Substances 0.000 claims abstract description 3
- 229940088597 hormone Drugs 0.000 claims abstract description 3
- 239000005720 sucrose Substances 0.000 claims description 12
- 238000005303 weighing Methods 0.000 claims description 12
- 238000002360 preparation method Methods 0.000 claims description 9
- 229910052799 carbon Inorganic materials 0.000 claims description 7
- 239000002994 raw material Substances 0.000 claims description 7
- 239000012452 mother liquor Substances 0.000 claims description 6
- 238000012856 packing Methods 0.000 claims description 6
- 239000007787 solid Substances 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 238000004659 sterilization and disinfection Methods 0.000 claims description 6
- 238000010257 thawing Methods 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 239000003104 tissue culture media Substances 0.000 claims description 5
- 238000012090 tissue culture technique Methods 0.000 claims description 4
- 150000001875 compounds Chemical class 0.000 claims description 3
- 238000012423 maintenance Methods 0.000 claims description 2
- 230000004069 differentiation Effects 0.000 abstract description 4
- 238000009395 breeding Methods 0.000 abstract description 2
- 230000001488 breeding effect Effects 0.000 abstract description 2
- 238000011081 inoculation Methods 0.000 abstract description 2
- 239000005971 1-naphthylacetic acid Substances 0.000 abstract 4
- IIDAJRNSZSFFCB-UHFFFAOYSA-N 4-amino-5-methoxy-2-methylbenzenesulfonamide Chemical compound COC1=CC(S(N)(=O)=O)=C(C)C=C1N IIDAJRNSZSFFCB-UHFFFAOYSA-N 0.000 abstract 4
- 229960004793 sucrose Drugs 0.000 abstract 2
- 241000196324 Embryophyta Species 0.000 description 6
- 238000010923 batch production Methods 0.000 description 2
- SQGYWYIDSFRISU-UHFFFAOYSA-N 6-benzyl-7h-purin-2-amine Chemical class C=12NC=NC2=NC(N)=NC=1CC1=CC=CC=C1 SQGYWYIDSFRISU-UHFFFAOYSA-N 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a method for preparing a monstera deliciosa plant tissue medium and relates to a plant tissue culture technology. The method for preparing the monstera deliciosa plant tissue medium adopts a tissue culture technology, a differential medium is prepared by adding hormone 6-benzyl aminopurine and 1-naphthaleneacetic acid in a Murashige and Skoog (MS) minimal medium, is used for breeding monstera deliciosa in a cluster bud manner and contributes to maintaining expectable characteristics. A rooting medium is prepared by adding the 1-naphthaleneacetic acid and activated carbon in a minimal medium 1/2 MS and can be used for improving the rooting quality. The formula of the monstera deliciosa plant tissue medium consists of the differential medium and the rooting medium; the formula of the differential medium comprises the minimal medium MS, 6-benzyl aminopurine, the 1-naphthaleneacetic acid, cane sugar and agar powder; the formula of the rooting medium comprises the minimal medium 1/2 MS, the 1-naphthaleneacetic acid, the activated carbon, the cane sugar and the agar powder; and a technology for preparing the monstera deliciosa plant tissue medium adopts a mode that the differential medium and the rooting medium are respectively prepared and applied. The culture period of the formula of the differential medium only takes about 20 days, the differentiation times are between 4-8, no variation is generated, and the seedling uniformity is high; and the rooting ratio of the formula of the rooting medium is high, inoculation takes 30 days, the rooting ratio reaches 98%, and the rooting number of single seedlings is between 4-6.
Description
Technical field
The invention belongs to agricultural biological technical field, relate to the tissue culture technique of a Plants.
Background technology
Monstera deliciosa originates in Mexico, is usually used in potted plant viewing and admiring in American-European, Japan, intersperses guest room and windowsill, and is comparatively general.The Mexico at South American nations Brazil, Argentina and middle part, America often plants by corridor or building except potted plant, allows Monstera deliciosa overgrow in frame or adhesion in wall, becomes fabulous vertical greening material.The Monstera deliciosa breeding is mainly in the cuttage mode as main, but the reproduction speed coefficient is low, and existing Monstera deliciosa tissue culture technique is immature, and coefficient of differentiation is low, and it is poor to take root, and young plant is irregular, can not satisfy the demand that batch production is produced.
Summary of the invention
The purpose of this invention is to provide a kind of Monstera deliciosa plant tissue culture media compound method, slow to solve existing Monstera deliciosa propagation method reproduction speed, can not satisfy the problem of Monstera deliciosa batch production Production requirement.
The technical solution adopted for the present invention to solve the technical problems is to adopt tissue culture technique, differential medium is to add hormone 6-benzyl aminopurine, 1-methyl α-naphthyl acetate in the MS minimal medium, mode with Multiple Buds is bred Monstera deliciosa, is conducive to the maintenance of merit.Root media is to add 1-methyl α-naphthyl acetate, active carbon in minimal medium 1/2MS, improves quality of rooting, and cultivating for Monstera deliciosa provides the seedling of taking root in a large number.The plant tissue culture media prescription of Monstera deliciosa is comprised of differential medium and root media, and two kinds of medium are prepared respectively, supporting application.Differential medium prescription: minimal medium MS, 6-benzyl aminopurine 6.0-10.0 mg/litre, 1-methyl α-naphthyl acetate 0.3-0.7 mg/litre, sucrose 25000-35000 mg/litre, agar powder 4000-6000 mg/litre; Prescription of rooting medium: minimal medium 1/2MS, 1-methyl α-naphthyl acetate 0.8-1.2 mg/litre, active carbon 2500-3500 mg/litre, sucrose 25000-35000 mg/litre, agar powder 4000-6000 mg/litre; Its preparation technology is as follows:
1, the preparation of differential medium prescription:
Add in the pot fill 0.8 liter of pure water by above-mentioned raw materials proportioning weighing agar powder and to boil; Add in the pot after getting 0.1 liter of 10 times of MS mother liquor and 6-benzyl aminopurine, the dissolving of 1-methyl α-naphthyl acetate; Weighing sucrose adds in the pot and allows its thawing; Constant volume to 1.0 liter; Adjusting the pH value is 5.8; 26 bottles of packing are carried out high temperature 121-126 ℃, high pressure 0.1-0.14MPa sterilization 15 minutes; Be cooled to solid; Connect aseptic seedling and carry out aseptic culture.
2, the preparation of prescription of rooting medium
Add in the pot fill 0.8 liter of pure water by above-mentioned raw materials proportioning weighing agar powder and to boil; Add in the pot after getting 0.05 liter of 10 times of MS mother liquor and 1-methyl α-naphthyl acetate, active carbon dissolving; Weighing sucrose adds in the pot and allows its thawing; Constant volume to 1.0 liter; Adjusting the pH value is 5.8; 26 bottles of packing are carried out high temperature 121-126 ℃, high pressure 0.1-0.14MPa sterilization 15 minutes; Be cooled to solid; Connect aseptic seedling and carry out aseptic culture.
Adopting good effect of the present invention is that this differential medium prescription only has about 20 days cultivation cycle, and the differentiation multiple is between 4-8, and not variation, and the young plant regularity of producing is high; The prescription of rooting medium rooting rate is high, after inoculation 30 days.Rooting rate is between 92-98%, and every seedling rooting number is between 4-6.
Embodiment
To prepare 1 liter of differential medium, 1 liter of root media is example, and its composition of raw materials proportioning is: differential medium: 0.1 liter in 10 times of MS mother liquors, 8 milligrams of 6-benzyl aminopurines, 0.5 milligram of 1-methyl α-naphthyl acetate, 30000 milligrams of sucrose, 5000 milligrams of agar powders; Root media: 0.05 liter in 10 times of MS mother liquors, 1 milligram of 1-methyl α-naphthyl acetate, 3000 milligrams of active carbons, 30000 milligrams of sucrose, 5000 milligrams of agar powders; Its preparation flow is as follows:
1, the preparation of differentiation based formulas:
Add in the pot fill 0.8 liter of pure water by above-mentioned raw materials proportioning weighing agar powder and to boil; Add in the pot after getting 0.1 liter of 10 times of MS mother liquor and 6-benzyl aminopurine, the dissolving of 1-methyl α-naphthyl acetate; Weighing sucrose adds in the pot and allows its thawing; Constant volume to 1 liter; Adjusting the pH value is 5.8; 26 bottles of packing are carried out high temperature 121-126 ℃, high pressure 0.1-0.14MPa sterilization 15 minutes; Be cooled to solid; Connect aseptic seedling and carry out aseptic culture.
2, the take root preparation of based formulas
Add in the pot fill 0.8 liter of pure water by above-mentioned raw materials proportioning weighing agar powder and to boil; MS mother liquor and 1-methyl α-naphthyl acetate, the active carbon of getting 0.05 liter 10 times dissolve rear the adding in the pot; Weighing sucrose adds in the pot and allows its thawing; Constant volume to 10 liter; Adjusting the pH value is 5.8; 26 bottles of packing are carried out high temperature 121-126 ℃, high pressure 0.1-0.14MPa sterilization 15 minutes; Be cooled to solid; Connect aseptic seedling and carry out aseptic culture.
Claims (1)
1. Monstera deliciosa plant tissue culture media compound method, it is characterized in that adopting tissue culture technique, differential medium is to add hormone 6-benzyl aminopurine, 1-methyl α-naphthyl acetate in the MS minimal medium, breeds Monstera deliciosa in the mode of Multiple Buds, is conducive to the maintenance of merit; Root media is to add 1-methyl α-naphthyl acetate, active carbon in minimal medium 1/2MS, improves quality of rooting, and cultivating for Monstera deliciosa provides the seedling of taking root in a large number; The plant tissue culture media prescription of Monstera deliciosa is comprised of differential medium and root media, and two kinds of medium are prepared respectively, supporting application; Differential medium prescription: minimal medium MS, 6-benzyl aminopurine 6.0-10.0 mg/litre, 1-methyl α-naphthyl acetate 0.3-0.7 mg/litre, sucrose 25000-35000 mg/litre, agar powder 4000-6000 mg/litre; Prescription of rooting medium: minimal medium 1/2MS, 1-methyl α-naphthyl acetate 0.8-1.2 mg/litre, active carbon 2500-3500 mg/litre, sucrose 25000-35000 mg/litre, agar powder 4000-6000 mg/litre; Its preparation technology is as follows:
(1) preparation of differential medium prescription:
A adds in the pot fill 0.8 liter of pure water by above-mentioned raw materials proportioning weighing agar powder and boils:
B gets 0.1 liter of 10 times of MS mother liquor and 6-benzyl aminopurine, the dissolving of 1-methyl α-naphthyl acetate is rear adds in the pot;
C weighing sucrose adds in the pot and allows its thawing;
D constant volume to 1.0 liter;
It is 5.8 that E adjusts the pH value;
26 bottles of F packing are carried out high temperature 121-126 ℃, high pressure 0.1-0.14MPa sterilization 15 minutes;
G is cooled to solid;
H connects aseptic seedling and carries out aseptic culture;
(2) preparation of prescription of rooting medium
A adds in the pot fill 0.8 liter of pure water by above-mentioned raw materials proportioning weighing agar powder and boils;
B gets 0.05 liter of 10 times of MS mother liquor and 1-methyl α-naphthyl acetate, the active carbon dissolving is rear adds in the pot;
C weighing sucrose adds in the pot and allows its thawing;
D constant volume to 1.0 liter;
It is 5.8 that E adjusts the pH value;
26 bottles of F packing are carried out high temperature 121-126 ℃, high pressure 0.1-0.14MPa sterilization 15 minutes;
G is cooled to solid;
H connects aseptic seedling and carries out aseptic culture.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012103838030A CN102845310A (en) | 2012-10-11 | 2012-10-11 | Method for preparing monstera deliciosa plant tissue medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012103838030A CN102845310A (en) | 2012-10-11 | 2012-10-11 | Method for preparing monstera deliciosa plant tissue medium |
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| CN102845310A true CN102845310A (en) | 2013-01-02 |
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| CN2012103838030A Pending CN102845310A (en) | 2012-10-11 | 2012-10-11 | Method for preparing monstera deliciosa plant tissue medium |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN119138332A (en) * | 2024-11-15 | 2024-12-17 | 西南林业大学 | Method for rapid propagation of tissue of monstera deliciosa and application |
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2012
- 2012-10-11 CN CN2012103838030A patent/CN102845310A/en active Pending
Non-Patent Citations (1)
| Title |
|---|
| 黄惠芳: "龟背竹快速繁殖试验", 《广西热作科技》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN119138332A (en) * | 2024-11-15 | 2024-12-17 | 西南林业大学 | Method for rapid propagation of tissue of monstera deliciosa and application |
| CN119138332B (en) * | 2024-11-15 | 2025-01-24 | 西南林业大学 | Method for rapid propagation of tissue of monstera deliciosa and application |
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