CN102827887B - Preparation method for structured phospholipids based on enzyme reactor - Google Patents
Preparation method for structured phospholipids based on enzyme reactor Download PDFInfo
- Publication number
- CN102827887B CN102827887B CN201210276840.1A CN201210276840A CN102827887B CN 102827887 B CN102827887 B CN 102827887B CN 201210276840 A CN201210276840 A CN 201210276840A CN 102827887 B CN102827887 B CN 102827887B
- Authority
- CN
- China
- Prior art keywords
- air
- retort
- enzyme
- inlet
- reactor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Thermal Sciences (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a preparation method for structured phospholipids based on an enzyme reactor. The invention provides the enzyme reactor used for structured phospholipids. The enzyme reactor comprises a reaction tank and an outer jacket; the reaction tank is provided with a material inlet, an enzyme feeding inlet, an air inlet, an air distributor, an air outlet and a material outlet; and the outer jacket is provided with a cooling water inlet and a cooling water outlet. A reaction material enters into the reaction tank from the material inlet at a feeding speed of 1 to 2 m/s; catalytic enzyme accounting for 10 to 20% of the mass of phospholipids enters into the reaction tank from the enzyme feeding inlet; purified air enters into the reaction tank from the air inlet and is dispersed into uniform small bubbles through the air distributor, and an air velocity is 1 to 3 m/s; after a reaction is finished, the material flows out from the material outlet, and an outflow speed is 1 to 2 m/s. The enzyme reactor used for structured phospholipids provided by the invention has the advantages of a simple structure, low cost, a large effective reaction volume and suitability for large-scale industrial production of structured phospholipids; with the enzyme reactor and the method, full circulation of the reaction material can be promoted, the reaction is conveniently and effectively controlled, and the conversion rate of unsaturated fatty acid is significantly improved.
Description
Technical field
The invention belongs to the preparing technical field of matter structure phosphatide, be specifically related to a kind of matter structure phosphatide preparing process based on enzyme reactor.
Background technology
Phosphatide is in molecule, contain one or more phosphates and have one of compound lipid of polarity.They are present in microbial world, vegitabilia and animal kingdom widely, are to form one of biomembranous basal component, all relevant with all kinds of substance metabolism activities relevant with life.But the saturated fatty acid in phospholipid molecule can improve cholesterol level harmful in blood, take in and too much can threaten to HUMAN HEALTH.
Matter structure phosphatide is under the katalysis of enzyme, by transesterification reaction, the unsaturated fatty acidss such as middle carbochain grease, conjugated linolic acid, plant sterol or n-3 series polyunsaturated fatty acid is introduced in phospholipid molecule, replace the saturated fatty acid in phospholipid molecule and obtain, thering is at present important researching value and demand is applied in increasingly extensive practicality.But existing scale operation matter structure phosphatide exists, and low conversion rate, temperature are wayward, complex operation, high in cost of production defect.
Research discovery, reactor has important effect to improving enzyme process reaction conversion ratio.< < chemical industry " is applied the research of multi-form reactor to bagasse enzymic hydrolysis " with the article that engineering > > the 6th phase of calendar year 2001 Zheng becomes, adopt tank reactor and fixed-bed reactor, as cellulase degradation Mierocrystalline cellulose, prepare the reactor of oligose.Tank reactor is simple in structure, flexible operation, but the method reaction times is long, energy-output ratio is large and product yield is lower; That immobilization bed bioreactor reaction has that cost is low, enzyme reclaims is easy, simple to operate, transformation efficiency is compared with advantages of higher, yet fixed-bed reactor heat transfer property is poor, the more difficult control of inside reactor temperature; < < food and machinery > > the 1st phase Wang Wen outstanding person's in 2008 article " research of hydrolyzed soy protein isolate in enzyme mebrane reactor ", adopt molecular weight cut-off 10kDa tangential flow filtration enzyme mebrane reactor, as the enzymolysis reactor of soybean protein isolate, prepare small molecules soybean polypeptide.The method technique is simple, product yield is higher.But the method also exists degradation defect under enzyme leakage and enzymic activity, cause reaction efficiency to decline and production cost increase.Application number is 201120017106.4 utility model patent " enzyme reactor ", designed and produced a kind of reactor of stirring-type, inside reactor temperature distribution is comparatively even, even if local temperature moment is too high, also can temperature be reduced by stirring, but this reactor useful volume reduces, and is unsuitable for large-scale production.Also the order and mode, the material that for the material in matter structure phosphatide preparation process, do not add and flow out add speed, cooling way to carry out integrated design.Have no at present the enzyme reactor that the suitable preparation feedback for matter structure phosphatide provides specially.
Summary of the invention
The object of the invention is to overcome low conversion rate in existing matter structure phosphatide technology of preparing, wayward, be unsuitable for the technical deficiencies such as scale operation, a kind of matter structure phosphatide preparing process based on enzyme reactor is provided, the inventive method transformation efficiency is high, control is convenient, running cost is cheap, and is applicable to the scale operation of matter structure phosphatide.
Object of the present invention is achieved by the following technical programs:
A kind of matter structure phosphatide preparing process based on enzyme reactor is provided, comprises the following steps:
(1) provide and prepare a germplasm structure phosphatide enzyme process reactor, reactor comprises retort and external jacket; External jacket is wrapped in the outside of retort, and external jacket is provided with cooling water inlet and cooling water outlet, and water coolant from then on cooling water inlet enters external jacket, after the cooling retort of whole external jacket of flowing through, from cooling water outlet, flows out;
Retort is provided with material inlet, for add phosphatide and the even liquid of unsaturated fatty acids mixing according to a certain percentage to retort; Be provided with enzyme-added mouthful, katalaze enzyme (lipase or Phospholipid hydrolase) enters retort from enzyme-added mouth by a certain percentage; Being provided with gas inlet and air-distributor, purifying air and enter from gas inlet the air-distributor of being located in retort, have some apertures on air-distributor, is that uniform small bubbles enter retort by air dispersion; Be provided with air vout, for the discharge of air in retort; Be provided with a material outlet, for the discharge of reaction solution;
(2) after material proportionally mixes, from the material inlet of retort, enter retort, the speed of material feeding is 1～2m/s, in 10～20% ratio of phosphatide quality in mixture, from enzyme-added mouthful, to retort, adds enzyme for catalysis (lipase or Phospholipid hydrolase);
(3) purify air and enter retort from gas inlet, air velocity is preferably 1～3m/s, purifies air and distributes and to become uniform bubble and enter reaction solution through air-distributor from gas inlet, and unnecessary air is discharged from air vout;
Small aperture on described air-distributor is preferably 2～4cm; Distribution density is preferably 150～400/m
2; More preferably, the small aperture on described air-distributor is 2.4～3.2cm.
Preferably, the speed of the described material feeding of step (2) is 1.2～1.6m/s;
Preferably, the described air velocity of step (3) is 1.5～2.5m/s;
According to the selection of the setting of air-distributor of the present invention and air velocity, the mode that reaction solution is realized also to flow in reactor circulates fully;
(4) water coolant passes into external jacket from cooling water inlet valve, and the Flow Velocity of water coolant is 1～3m/s, and water coolant is discharged from cooling sea water outlet valve, plays the effect of cooling reactor internal temperature;
(5) after reaction finishes, reaction solution flows out from material outlet, and the speed that reaction solution flows out is 1～2m/s.
Preferably, in the middle of the retort of described enzyme process reactor, be cylindrical, top and bottom are semisphere, and air vout is located at retort top, and enzyme-added mouth and material inlet are divided into air vout both sides; Gas inlet is located at the bottom of retort, and gas inlet connects the onesize circular air-distributor of internal diameter, the aperture that distributing equably on air-distributor, and the bottom of retort is provided with material outlet.
Preferably, the height of the retort of described matter structure phosphatide enzyme process reactor is 5～10m, and diameter is 2～4m; Retort top material inlet internal diameter is 10～20cm, and the enzyme-added mouthful of internal diameter on retort top is 20～40cm, and air vout internal diameter is 12～18cm.The internal diameter of gas inlet is 5～10cm, and the bottom material outlet internal diameter of retort is 15～25cm; External jacket is wrapped in the outside of retort, and the cooling water inlet on external jacket and cooling water outlet internal diameter are 7～12cm.
More preferably, the height of above-mentioned retort is 6m, and diameter is 3m.Retort top material inlet internal diameter is 15cm, and the enzyme-added mouthful of internal diameter on retort top is 30cm, and air vout internal diameter is 25cm.The internal diameter of gas inlet is 8cm, and the bottom material outlet internal diameter of retort is 20cm.External jacket is wrapped in the outside of retort, and the cooling water inlet on external jacket and cooling water outlet internal diameter are 8cm.On air-distributor, the internal diameter of each aperture the best is 3cm, and density the best of little pore distribution is 240/m
2, realize preferably reaction solution circulating effect.
The invention has the beneficial effects as follows:
Based on enzyme process reactor of the present invention and exactly determined material adds and outflow mode, add and take-off rate, the critical technical parameter such as cooling, the invention provides a kind of global optimization matter structure phosphatide enzyme process reaction method for preparing.By pass into purifying air of suitable flow velocity in reactor, promote the circulation of reaction solution, significantly improve the catalytic efficiency of enzyme, guaranteed higher transformation efficiency; Accelerated the thermal cycling of inside reactor simultaneously, and in external jacket in being used in conjunction with of cool circulation water of suitable flow velocity, can control easily and effectively the temperature of reaction system.The inventive method production cost is lower, and energy consumption is few, and in retort, useful volume is very large, is suitable for the production of mass-producing, but also can be used as other reactors or fermentor tank.
Accompanying drawing explanation
Fig. 1 matter structure of the present invention phosphatide enzyme process structure of reactor schematic diagram
The vertical view of Fig. 2 reactor of the present invention
The structural representation of Fig. 3 air-distributor of the present invention
Embodiment
Below in conjunction with the drawings and specific embodiments, describe the present invention in detail.The embodiment of the present invention is intended to illustrate that enzyme process structure of reactor that the present invention designs is combined the remarkable technique effect that the overall technical architecture that obtains brings with critical processes such as described input and output material speed, as for adopt any phosphatide as reaction raw materials, adopt any enzyme can be conventional with reference to the art as catalyzer, the concrete proportioning of raw material etc., at this, do not repeat one by one, but therefore do not limit the present invention.
Embodiment 1
As shown in Figure 1, the present invention is based on a kind of new enzyme process reactor (1), reactor (1) comprises retort (2) and external jacket (3).Retort (2) is cylindrical, top and bottom and be semisphere, and the height of retort (2) is 6m, diameter is 3m.Retort (2) top material inlet (4) internal diameter is 10cm, and enzyme-added mouthful of (5) internal diameter on retort (2) top is 25cm, and air vout (7) internal diameter is 12cm.A gas inlet (6) is arranged at the bottom of retort (2), the internal diameter of gas inlet (6) is 5cm, gas inlet (6) the onesize circular air-distributor (8) of internal diameter of ining succession, the internal diameter that distributing uniformly on air-distributor (8) is 2cm aperture, 200/m of aperture distribution density
2, bottom material outlet (9) internal diameter of retort (2) is 15cm.External jacket (3) is wrapped in the outside of retort (2), and on external jacket, the cooling water inlet of (3) (10) are 8cm with cooling water outlet (11) internal diameter.
After soybean phospholipid mixes by a certain percentage with linolic acid, from material inlet (4), enter retort (2), the speed of charging is 1m/s; In 10% ratio of phosphatide quality in mixture, from enzyme-added mouthful (5), to retort, add enzyme for catalysis (Novozym435 lipase)
Air after purification enters retort (2) from gas inlet (6), and air velocity is 1m/s, becomes uniform bubble enter reaction solution by air-distributor (8), promotes the circulation of reaction solution, and reaction solution circulates in the mode also flowing in reactor.Air is discharged from air vout (7).
From cooling water inlet, the water valve of (10) passes into external jacket to water coolant, and the flow velocity of water coolant is 1m/s, and water coolant is discharged from the water valve of cooling water outlet (11).
After reaction finishes, reaction solution flows out from material outlet (9), and the speed that reaction solution flows out is 1m/s.
The present invention is through global optimization and the appropriate design of reaction vessel and reaction process condition; successfully break away from mechanical stirring device; expanded the useful volume of reaction vessel; promoted the circulation of reaction solution; significantly improve the catalytic efficiency of enzyme; guaranteed higher transformation efficiency; in effective quickening of simultaneous reactions device internal heat circulation and external jacket, the cool circulation water of suitable flow velocity is used in conjunction with; can control easily and effectively the temperature of reaction system; energy consumption is few; production cost is lower, is suitable for the production of mass-producing.From the present embodiment experiment effect, the productive rate of matter structure phosphatide reaches 67%, and the productive rate that under equal conditions tank reactor is prepared matter structure phosphatide is only 45%.
Embodiment 2
The present embodiment provides a germplasm structure phosphatide enzyme process reactor (1), and reactor (1) comprises retort (2) and external jacket (3).Retort (2) is cylindrical, top and bottom and be semisphere, and the height of retort (2) is 8m, diameter is 3m.Material inlet (4) internal diameter at retort (2) top is 20cm, and enzyme-added mouthful of (5) internal diameter at retort (2) top is 40cm, and air vout (7) internal diameter is 15cm.A gas inlet (6) is arranged at the bottom of retort (2), the internal diameter of gas inlet (6) is 10cm, gas inlet (6) the onesize circular air-distributor (8) of internal diameter of ining succession, the internal diameter that distributing uniformly on air-distributor (8) is 3cm aperture, 200/m of aperture distribution density
2, bottom material outlet (9) internal diameter of retort (2) is 20cm.External jacket (3) is wrapped in the outside of retort (2), and the cooling water inlet (10) on external jacket (3) is 10cm with cooling water outlet (11) internal diameter.
After soybean phospholipid mixes by a certain percentage with linolic acid, from material inlet (4), enter retort (2), the speed of charging is 1m/s; In 10% ratio of phosphatide quality in mixture, from enzyme-added mouthful (5), to retort, add enzyme for catalysis (Novozym435 lipase)
Air after purification enters retort (2) from gas inlet (6), and air velocity is 1m/s, becomes uniform bubble enter reaction solution by air-distributor (8), promotes the circulation of reaction solution, and reaction solution circulates in the mode also flowing in reactor.Air is discharged from air vout (7).
From cooling water inlet, the water valve of (10) passes into external jacket (3) to water coolant, and the flow velocity of water coolant is 1m/s, and water coolant is discharged from the water valve of cooling water outlet (11).
After reaction finishes, reaction solution flows out from material outlet (9), and the speed that reaction solution flows out is 1m/s.
The present invention, through global optimization and the appropriate design of reaction vessel and reaction process condition, successfully breaks away from mechanical stirring device, has expanded the useful volume of reaction vessel, has promoted the circulation of reaction solution, significantly improves the catalytic efficiency of enzyme, has guaranteed higher transformation efficiency.Being used in conjunction with of the cool circulation water of suitable flow velocity in effective quickening of simultaneous reactions device internal heat circulation and external jacket, can control the temperature of reaction system easily and effectively, and energy consumption is few, and production cost is lower, is suitable for the production of mass-producing.From the present embodiment effect, the productive rate of the present embodiment matter structure phosphatide reaches 68%, and the productive rate that under equal conditions tank reactor is prepared matter structure phosphatide is only 45%.
Embodiment 3
The present embodiment provides a germplasm structure phosphatide enzyme process reactor (1), and reactor (1) comprises retort (2) and external jacket (3).Retort (2) is cylindrical, top and bottom and be semisphere, and the height of retort (2) is 8m, diameter is 3m.Material inlet (4) internal diameter at retort (2) top is 15cm, and enzyme-added mouthful of (5) internal diameter at retort (2) top is 30cm, and air vout (7) internal diameter is 15cm.A gas inlet (6) is arranged at the bottom of retort (2), the internal diameter of gas inlet (6) is 10cm, gas inlet (6) connects the onesize circular air-distributor (8) of internal diameter, the internal diameter that distributing uniformly on air-distributor (8) is 3cm aperture, and aperture distribution density is 300/m
2, bottom material outlet (9) internal diameter of retort (2) is 20cm.External jacket (3) is wrapped in the outside of retort (2), and the cooling water inlet (10) on external jacket (3) is 10cm with cooling water outlet (11) internal diameter.
After soybean phospholipid mixes by a certain percentage with linolic acid, from material inlet (4), enter retort (2), the speed of charging is 1.5m/s; In 20% ratio of phosphatide quality in mixture, from enzyme-added mouthful (5), to retort (2), add enzyme for catalysis (Novozym435 lipase)
Air after purification enters retort (2) from gas inlet (6), and air velocity is 2m/s, becomes uniform bubble enter reaction solution by air-distributor (8), promotes the circulation of reaction solution, and reaction solution circulates in the mode also flowing in reactor.Air is discharged from air vout (7).
From cooling water inlet, the water valve of (10) passes into external jacket (3) to water coolant, and the flow velocity of water coolant is 2m/s, and water coolant is discharged from the water valve of cooling water outlet (11).
After reaction finishes, reaction solution flows out from material outlet (9), and the speed that reaction solution flows out is 1.8m/s.
Based on the present embodiment reactor and reaction method, the productive rate of matter structure phosphatide can reach 71%, and the productive rate that under equal conditions tank reactor is prepared matter structure phosphatide is only 48%.
Embodiment 4
As shown in Figure 1, the present invention is based on a kind of new enzyme process reactor (1), reactor (1) comprises retort (2) and external jacket (3).Retort (2) is cylindrical, top and bottom and be semisphere, and the height of retort (2) is 6m, diameter is 3m.Retort (2) top material inlet (4) internal diameter is 20cm, and enzyme-added mouthful of (5) internal diameter on retort (2) top is 40cm, and air vout (7) internal diameter is 18cm.A gas inlet (6) is arranged at the bottom of retort (2), the internal diameter of gas inlet (6) is 10cm, gas inlet (6) the onesize circular air-distributor (8) of internal diameter of ining succession, the internal diameter that distributing uniformly on air-distributor (8) is 2cm aperture, 400/m of aperture distribution density
2; Bottom material outlet (9) internal diameter of retort (2) is 25cm.External jacket (3) is wrapped in the outside of retort (2), and on external jacket, the cooling water inlet of (3) (10) are 12cm with cooling water outlet (11) internal diameter.
After soybean phospholipid mixes by a certain percentage with linolic acid, from material inlet (4), enter retort (2), the speed of charging is 2m/s; In 15% ratio of phosphatide quality in mixture, from enzyme-added mouthful, in retort, add enzyme for catalysis (Novozym435 lipase)
Air after purification enters retort (2) from gas inlet (6), and air velocity is 3m/s, becomes uniform bubble enter reaction solution by air-distributor (8), promotes the circulation of reaction solution, and reaction solution circulates in the mode also flowing in reactor.Air is discharged from air vout (7).
From cooling water inlet, the water valve of (10) passes into external jacket (3) to water coolant, and the flow velocity of water coolant is 3m/s, and water coolant is discharged from the water valve of cooling water outlet (11).
After reaction finishes, reaction solution flows out from material outlet (9), and the speed that reaction solution flows out is 2m/s.
Based on the present embodiment reactor and reaction method, the productive rate of matter structure phosphatide can reach 74%, and the productive rate that under equal conditions tank reactor is prepared matter structure phosphatide is only 51%.
embodiment 5
As shown in Figure 1, the present invention is based on a kind of new enzyme process reactor (1), reactor (1) comprises retort (2) and external jacket (3).Retort (2) is cylindrical, top and bottom and be semisphere, and the height of retort (2) is 6m, diameter is 3m.Retort (2) top material inlet (4) internal diameter is 15cm, and enzyme-added mouthful of (5) internal diameter on retort (2) top is 30cm, and air vout (7) internal diameter is 20cm.A gas inlet (6) is arranged at the bottom of retort (2), the internal diameter of gas inlet (6) is 8cm, gas inlet (6) the onesize circular air-distributor (8) of internal diameter of ining succession, the internal diameter that distributing uniformly on air-distributor (8) is 3cm aperture, 240/m of aperture distribution density
2; Bottom material outlet (9) internal diameter of retort (2) is 20cm.External jacket (3) is wrapped in the outside of retort (2), and on external jacket, the cooling water inlet of (3) (10) are 8cm with cooling water outlet (11) internal diameter.
After soybean phospholipid mixes by a certain percentage with linolic acid, from material inlet (4), enter retort (2), the speed of charging is 1.5m/s; In 15% ratio of phosphatide quality in mixture, from enzyme-added mouthful, in retort, add enzyme for catalysis (Novozym435 lipase)
Air after purification enters retort (2) from gas inlet (6), and air velocity is 2.5m/s, becomes uniform bubble enter reaction solution by air-distributor (8), promotes the circulation of reaction solution, and reaction solution circulates in the mode also flowing in reactor.Air is discharged from air vout (7).
From cooling water inlet, the water valve of (10) passes into external jacket (3) to water coolant, and the flow velocity of water coolant is 3m/s, and water coolant is discharged from the water valve of cooling water outlet (11).
After reaction finishes, reaction solution flows out from material outlet (9), and the speed that reaction solution flows out is 2m/s.
The present embodiment is further to the more excellent design of the carrying out of reaction vessel and reaction process condition, and based on the present embodiment reactor and reaction method, the productive rate of matter structure phosphatide can reach 76%, and the productive rate that under equal conditions tank reactor is prepared matter structure phosphatide is only 51%.
In sum, the present invention, by passing into and purify air in reactor, promotes the circulation of reaction solution, improved the reaction efficiency of lipase, accelerated the thermal cycling of inside reactor simultaneously, by with being used in conjunction with of cool circulation water, can effectively control the inner temperature of reaction.In addition, reactor production cost provided by the invention is lower, and energy consumption is few, and in retort, useful volume is very large, is suitable for large-scale production, but also can be used as other reactors or fermentor tank.
Claims (4)
1. the matter structure phosphatide preparing process based on enzyme reactor, is characterized in that comprising the following steps:
(1) prepare matter structure phosphatide enzyme process reactor;
Described matter structure phosphatide enzyme process reactor comprises retort and external jacket; External jacket is wrapped in the outside of retort, and external jacket is provided with cooling water inlet and cooling water outlet, and water coolant enters external jacket from cooling water inlet, after the cooling retort of whole external jacket of flowing through, from cooling water outlet, flows out;
Retort is provided with material inlet, for add phosphatide and unsaturated fatty acids mixed solution to retort; Be provided with enzyme-added mouthful, katalaze enzyme enters retort from enzyme-added mouth; Be provided with gas inlet and air-distributor, purify air and enter from gas inlet the air-distributor of being located at retort bottom, air-distributor is provided with some apertures, by air dispersion, is that uniform small bubbles enter retort; Be provided with air vout, in retort, from then on air discharges; Be provided with material outlet, reaction solution is discharged from material outlet;
In the middle of the retort of described matter structure phosphatide enzyme process reactor, be cylindrical, top and bottom are semisphere, and air vout is located at retort top, and enzyme-added mouth and material inlet are divided into air vout both sides; Gas inlet is located at the bottom of retort, and gas inlet connects air-distributor, and gas inlet is identical with air-distributor internal diameter, and air-distributor is circular, is uniformly distributed aperture on it, and the bottom of retort is provided with material outlet; Small aperture on described air-distributor is 2～4cm; Distribution density is 150～400/m
2;
The height of the retort of described matter structure phosphatide enzyme process reactor is 5～10m, and diameter is 2～4m; Retort top material inlet internal diameter is 10～20cm, and the enzyme-added mouthful of internal diameter on retort top is 20～40cm, and air vout internal diameter is 12～18cm; The internal diameter of gas inlet is 5～10cm, and the bottom material outlet internal diameter of retort is 15～25cm; Cooling water inlet on external jacket and cooling water outlet internal diameter are 7～12cm;
(2) after material proportionally mixes, from material inlet, enter retort, the speed of material feeding is 1～2m/s, then in 10～20% ratio of phosphatide quality in mixture, from enzyme-added mouthful, to retort, adds katalaze enzyme;
(3) purify air and enter retort from the gas inlet of reactor, air velocity is 1～3m/s, purifies air and distributes and to become uniform bubble and enter reaction solution through air-distributor from gas inlet, and unnecessary air is discharged from air vout;
(4) water coolant passes into external jacket from cooling water inlet valve, and the flow velocity of water coolant is 1～3m/s, and water coolant is discharged from cooling sea water outlet valve;
(5) after reaction finishes, reaction solution flows out from material outlet, and the speed that reaction solution flows out is 1～2m/s.
2. the matter structure phosphatide preparing process based on enzyme reactor according to claim 1, is characterized in that the speed of the described material feeding of step (2) is 1.2～1.6m/s.
3. the matter structure phosphatide preparing process based on enzyme reactor according to claim 1, is characterized in that the small aperture on described air-distributor is 2.4～3.2cm.
4. the matter structure phosphatide preparing process based on enzyme reactor according to claim 1, is characterized in that the described air velocity of step (3) is 1.5～2.5m/s.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210276840.1A CN102827887B (en) | 2012-08-06 | 2012-08-06 | Preparation method for structured phospholipids based on enzyme reactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210276840.1A CN102827887B (en) | 2012-08-06 | 2012-08-06 | Preparation method for structured phospholipids based on enzyme reactor |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102827887A CN102827887A (en) | 2012-12-19 |
| CN102827887B true CN102827887B (en) | 2014-07-16 |
Family
ID=47331195
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210276840.1A Expired - Fee Related CN102827887B (en) | 2012-08-06 | 2012-08-06 | Preparation method for structured phospholipids based on enzyme reactor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102827887B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106834117B (en) * | 2015-10-27 | 2018-11-23 | 苏州迪欧益生物科技有限公司 | For releasing the enzyme reactor and intelligent enzyme reaction work station of zooblast attaching |
| CN107551961B (en) * | 2017-11-03 | 2022-10-25 | 河北科技大学 | High-temperature high-pressure slurry bed reaction device |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101701229A (en) * | 2009-11-17 | 2010-05-05 | 广州海莎生物科技有限公司 | Method for preparing texture phospholipid and lecithin |
| CN201924022U (en) * | 2011-01-20 | 2011-08-10 | 江南大学 | Enzyme reactor |
-
2012
- 2012-08-06 CN CN201210276840.1A patent/CN102827887B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101701229A (en) * | 2009-11-17 | 2010-05-05 | 广州海莎生物科技有限公司 | Method for preparing texture phospholipid and lecithin |
| CN201924022U (en) * | 2011-01-20 | 2011-08-10 | 江南大学 | Enzyme reactor |
Non-Patent Citations (7)
| Title |
|---|
| Modification of Menhaden Oil by Enzymatic Acidolysis to Produce Structured Lipids: Optimization by Response Surface Design in a Packed Bed Reactor;Xuebing Xu et al.;《JAOCS》;20000228;171-176 * |
| Production of Structured Lipids by Lipase-Catalyzed Interesterification in a Flat Membrane Reactor;X. Xu et al.;《JAOCS》;20001031;1035-1041 * |
| X. Xu et al..Production of Structured Lipids by Lipase-Catalyzed Interesterification in a Flat Membrane Reactor.《JAOCS》.2000,1035-1041. |
| Xuebing Xu et al..Modification of Menhaden Oil by Enzymatic Acidolysis to Produce Structured Lipids: Optimization by Response Surface Design in a Packed Bed Reactor.《JAOCS》.2000,171-176. |
| 广州海莎生物科技有限公司.质构大豆磷脂关键技术的研究可行性报告.《百度文库》.2012,"具体研究开发内容和重点解决的技术关键问题"，图1-2. |
| 梅乐和，岑沛霖.酶反应器的设计和放大.《现代酶工程》.2006,第4章，第4.1、4.4、4.5节. * |
| 质构大豆磷脂关键技术的研究可行性报告;广州海莎生物科技有限公司;《百度文库》;20120710;"具体研究开发内容和重点解决的技术关键问题"，图1-2 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102827887A (en) | 2012-12-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US9187723B2 (en) | Algae processing | |
| CA2916891C (en) | Methods for the biomethanation of h2 and co2 | |
| Chai et al. | Enhanced removal of carbon dioxide and alleviation of dissolved oxygen accumulation in photobioreactor with bubble tank | |
| CN102108333B (en) | Fluidized bed bioreactor | |
| CN114127248A (en) | Bioreactor for growing microorganisms | |
| CN102827887B (en) | Preparation method for structured phospholipids based on enzyme reactor | |
| Leonzio | Studies of mixing systems in anaerobic digesters using CFD and the future applications of nanotechnologies | |
| WO2009078290A1 (en) | Continuous production of biodiesel fuel by enzymatic method | |
| CN102127505B (en) | Immobilized cell bioreactor | |
| CN1821123B (en) | Device and method for anaerobic treating waste water and method of propagating active granular bacteria strain at normal termperature | |
| RU2664860C1 (en) | Bioreactor for the intensive process of growing aerobic microorganisms | |
| CN104031834A (en) | Hydrogen manufacturing method through photosynthetic bacterium continuous reaction | |
| CN208038239U (en) | A kind of landfill leachate biochemical treatment system | |
| Wang et al. | Microfluidic tools for lipid production and modification: a review | |
| CN109868208A (en) | Bacterium algae integral bio energy generation device | |
| Ali et al. | Different type of industrial fermentors and their associated operations for the mass production of metabolite | |
| Thatoi et al. | Innovations in fermentation and phytopharmaceutical technologies | |
| CN202989099U (en) | Device for continuously producing molecular distillation monoglyceride by using immobilization bed | |
| CN109251847A (en) | Utilize the device and method of sunlight culture photosynthetic microorganism | |
| RU2595426C1 (en) | Method of processing organic wastes and biogas plant therefor | |
| CN110387315A (en) | Microalgae adsorbs the device and method of carbon dioxide preparation biodiesel | |
| Singhal et al. | Fermentation Technology Prospecting on Bioreactors/Fermenters: Design and Types | |
| CN202272864U (en) | Reaction zone device for optical energy biological culture system and photoreactor with reaction zone device | |
| Kaur et al. | Cultivation strategies with special reference to bioreactor design and operation for industrial production in biotechnology | |
| CN202063893U (en) | Complete equipment for cleaning crude methyl ester in production of biodiesel |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140716 Termination date: 20190806 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |