CN102827887A - Preparation method for structured phospholipids based on enzyme reactor - Google Patents
Preparation method for structured phospholipids based on enzyme reactor Download PDFInfo
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- CN102827887A CN102827887A CN2012102768401A CN201210276840A CN102827887A CN 102827887 A CN102827887 A CN 102827887A CN 2012102768401 A CN2012102768401 A CN 2012102768401A CN 201210276840 A CN201210276840 A CN 201210276840A CN 102827887 A CN102827887 A CN 102827887A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
Abstract
The invention discloses a preparation method for structured phospholipids based on an enzyme reactor. The invention provides the enzyme reactor used for structured phospholipids. The enzyme reactor comprises a reaction tank and an outer jacket; the reaction tank is provided with a material inlet, an enzyme feeding inlet, an air inlet, an air distributor, an air outlet and a material outlet; and the outer jacket is provided with a cooling water inlet and a cooling water outlet. A reaction material enters into the reaction tank from the material inlet at a feeding speed of 1 to 2 m/s; catalytic enzyme accounting for 10 to 20% of the mass of phospholipids enters into the reaction tank from the enzyme feeding inlet; purified air enters into the reaction tank from the air inlet and is dispersed into uniform small bubbles through the air distributor, and an air velocity is 1 to 3 m/s; after a reaction is finished, the material flows out from the material outlet, and an outflow speed is 1 to 2 m/s. The enzyme reactor used for structured phospholipids provided by the invention has the advantages of a simple structure, low cost, a large effective reaction volume and suitability for large-scale industrial production of structured phospholipids; with the enzyme reactor and the method, full circulation of the reaction material can be promoted, the reaction is conveniently and effectively controlled, and the conversion rate of unsaturated fatty acid is significantly improved.
Description
Technical field
The invention belongs to the preparing technical field of matter structure phosphatide, be specifically related to a kind of matter structure phosphatide preparing process based on enzyme reactor.
Background technology
Phosphatide is to contain one or more phosphates in the molecule and one of polar compound lipid is arranged.They are present in microbial world, vegitabilia and animality widely, are to constitute one of biomembranous basal component, and are all relevant with all kinds of substance metabolism activities relevant with life.But the sfas in the phospholipid molecule can improve deleterious cholesterol level in the blood, and Excessive Intake can threaten to HUMAN HEALTH.
Matter structure phosphatide is under the katalysis of enzyme, through transesterification reaction unsaturated fatty acidss such as middle carbochain grease, conjugated linolic acid, plant sterol or n-3 series pufas is introduced in the phospholipid molecule; Replacement in the phospholipid molecule sfas and obtain, have important researching value at present and demand is applied in increasingly extensive practicality.But existing scale operation matter structure phosphatide exists defectives such as transformation efficiency is low, temperature is wayward, complex operation, cost height.
Discover that reactor drum has important effect to improving the enzyme process reaction conversion ratio." chemical industry and engineering " the 6th phase of calendar year 2001 article of becoming of Zheng " is used the research of multi-form reactor drum to the bagasse enzymic hydrolysis ", adopts tank reactor and fixed-bed reactor, as the reactor drum of cellulase degradation preparation of cellulose oligose.Simple in structure, the flexible operation of tank reactor, but this method reaction times is long, energy-output ratio is big and product yield is lower; That immobilization bed bioreactor reaction has that cost is low, enzyme reclaims is easy, simple to operate, transformation efficiency is than advantages such as height, yet the fixed-bed reactor heat transfer property is relatively poor, the difficult control of inside reactor temperature; " food and machinery " outstanding article " research of hydrolyzed soy protein isolates in the enzyme mebrane reactor " of the 1st phase Wang Wen in 2008; Adopt molecular weight cut-off 10kDa tangential flow filtration enzyme mebrane reactor, prepare the small molecules soybean polypeptide as the enzymolysis reactor of soybean protein isolate.This method technology is simple, product yield is higher.But also there is degradation defective under enzyme leakage and the enzymic activity in this method, causes reaction efficiency to descend and the production cost increase.Application number is 201120017106.4 utility model patent " enzyme reactor "; Designed and produced a kind of reactor drum of stirring-type; The inside reactor temperature distribution is comparatively even, even if local temperature moment is too high, also can temperature be reduced through stirring; But this reactor drum useful volume reduces, and is inappropriate for large-scale production.Also not preparing material in the process to matter structure phosphatide adds with effusive order and mode, material adding speed, cooling way and carries out integrated design.Do not see at present the enzyme reactor that the suitable preparation feedback to matter structure phosphatide provides specially.
Summary of the invention
The objective of the invention is to overcome in the existing matter structure phosphatide technology of preparing transformation efficiency low, wayward, be inappropriate for technical deficiency such as scale operation; A kind of matter structure phosphatide preparing process based on enzyme reactor is provided; The inventive method transformation efficiency is high, control is convenient, running cost is cheap, and is applicable to the scale operation of matter structure phosphatide.
The object of the invention is achieved through following technical scheme:
A kind of matter structure phosphatide preparing process based on enzyme reactor is provided, may further comprise the steps:
(1) provide and prepare a germplasm structure phosphatide enzyme process reactor drum, reactor drum comprises retort and external jacket; External jacket is wrapped in the outside of retort, and external jacket is provided with cooling water inlet and cooling water outlet, and water coolant from then on cooling water inlet gets into external jacket, flows out from cooling water outlet behind the whole external jacket cooling reaction jar of flowing through;
Retort is provided with material inlet, is used for adding blended phosphatide and the even liquid of unsaturated fatty acids according to a certain percentage to retort; Be provided with enzyme-added mouthful, katalaze(enzyme) (lypase or Phospholipid hydrolase) gets into retort from enzyme-added mouthful by a certain percentage; Being provided with gas inlet and air-distributor, purifying air and be located at the air-distributor in the retort from the gas inlet entering, have some apertures on the air-distributor, is that uniform small bubbles get into retort with air dispersion; Be provided with air, be used for the discharge of air in the retort; Be provided with a material outlet, be used for the discharge of reaction solution;
(2) after material proportionally mixes; Get into retort from the material inlet of retort; The speed of material charging is 1～2m/s, adds catalysis with enzyme (lypase or Phospholipid hydrolase) in 10～20% ratio of phosphatide quality in the mixture from enzyme-added mouthful to retort;
(3) purify air from gas inlet entering retort, air velocity is preferably 1～3m/s, and purifying air becomes uniform bubble entering reaction solution from gas inlet through the air-distributor distribution, and unnecessary air is discharged from air;
Small aperture on the said air-distributor is preferably 2～4cm; Distribution density is preferably 150～400/m
2More preferably, the small aperture on the said air-distributor is 2.4～3.2cm.
Preferably, the speed of the said material charging of step (2) is 1.2～1.6m/s;
Preferably, the said air velocity of step (3) is 1.5～2.5m/s;
According to the setting of air-distributor according to the invention and the selection of air velocity, reaction solution is realized circulating fully with the mode that also flows in reactor drum;
(4) valve feeds external jacket to water coolant from the cooling water inlet, and the flow velocity degree of water coolant is 1～3m/s, and water coolant is discharged from cooling sea water outlet valve, plays the effect of cooling reactor internal temperature;
(5) after reaction finished, reaction solution flowed out from material outlet, and the effusive speed of reaction solution is 1～2m/s.
Preferably, be the cylinder bodily form in the middle of the retort of said enzyme process reactor drum, top and bottom are semisphere, and air is located at the retort top, and enzyme-added mouth and material inlet are divided into the air both sides; Gas inlet is located at the bottom of retort, and gas inlet connects the onesize circular air-distributor of internal diameter, the aperture that distributing equably on the air-distributor, and the bottom of retort is provided with material outlet.
Preferably, the height of the retort of said matter structure phosphatide enzyme process reactor drum is 5～10m, and diameter is 2～4m; Retort top material inlet internal diameter is 10～20cm, and the vertical enzyme-added mouthful of internal diameter of retort is 20～40cm, and the air internal diameter is 12～18cm.The internal diameter of gas inlet is 5～10cm, and the bottom material outlet internal diameter of retort is 15～25cm; External jacket is wrapped in the outside of retort, and cooling water inlet on the external jacket and cooling water outlet internal diameter are 7～12cm.
More preferably, the height of above-mentioned retort is 6m, and diameter is 3m.Retort top material inlet internal diameter is 15cm, and the vertical enzyme-added mouthful of internal diameter of retort is 30cm, and the air internal diameter is 25cm.The internal diameter of gas inlet is 8cm, and the bottom material outlet internal diameter of retort is 20cm.External jacket is wrapped in the outside of retort, and cooling water inlet on the external jacket and cooling water outlet internal diameter are 8cm.The internal diameter of each aperture the best is 3cm on the air-distributor, and density the best of little pore distribution is 240/m
2, realize preferable reaction solution circulating effect.
The invention has the beneficial effects as follows:
Add and outflow mode, adding and critical technical parameters such as take-off rate, cooling based on enzyme process reactor drum according to the invention and exactly determined material, the invention provides a kind of global optimization matter structure phosphatide enzyme process reaction method for preparing.Through in reactor drum, feeding purifying air of suitable flow velocity, promote the circulation of reaction solution, significantly improve the catalytic efficiency (of enzyme, guaranteed higher transformation efficiency; Accelerated the thermal cycling of inside reactor simultaneously, and in external jacket in being used of cool circulation water of suitable flow velocity, can control the temperature of reaction system easily and effectively.The inventive method production cost is lower, and energy consumption is few, and useful volume is very big in the retort, is suitable for the production of mass-producing, but also can be used as other reaction kettles or fermentor tank.
Description of drawings
Fig. 1 matter structure of the present invention phosphatide enzyme process structure of reactor synoptic diagram
The vertical view of Fig. 2 reactor drum of the present invention
The structural representation of Fig. 3 air-distributor of the present invention
Embodiment
Specify the present invention below in conjunction with accompanying drawing and specific embodiment.The embodiment of the invention is intended to explain the remarkable technique effect that overall technical architecture that enzyme process structure of reactor that the present invention designs combines with critical processes such as described input and output material speed to obtain is brought; Can be conventional as catalyzer, the concrete proportioning of raw material etc. as for any phosphatide of employing with reference to the present technique field as reaction raw materials, any enzyme of employing; Do not give unnecessary details one by one at this, but therefore do not limit the present invention.
Shown in accompanying drawing 1, the present invention is based on a kind of new enzyme process reactor drum (1), reactor drum (1) comprises retort (2) and external jacket (3).Retort (2) is cylindrical, top and bottom be semisphere, the height of retort (2) is 6m, diameter is 3m.Retort (2) top material inlet (4) internal diameter is 10cm, and vertical enzyme-added mouthful of (5) internal diameter of retort (2) is 25cm, and air (7) internal diameter is 12cm.A gas inlet (6) is arranged at the bottom of retort (2); The internal diameter of gas inlet (6) is 5cm; Gas inlet (6) the onesize circular air-distributor (8) of internal diameter of ining succession, the internal diameter that distributing uniformly on the air-distributor (8) is the 2cm aperture, 200/m of aperture distribution density
2, bottom material outlet (9) internal diameter of retort (2) is 15cm.External jacket (3) is wrapped in the outside of retort (2), and the cooling water inlet of (3) (10) are 8cm with cooling water outlet (11) internal diameter on the external jacket.
After soybean phospholipid and linolic acid mix by a certain percentage, get into retort (2) from material inlet (4), the speed of charging is 1m/s; 10% ratio in phosphatide quality in the mixture adds catalysis with enzyme (Novozym435 lypase) from enzyme-added mouthful (5) to retort
Cleaned air passes gets into retort (2) from gas inlet (6), and air velocity is 1m/s, becomes uniform bubble through air-distributor (8) and gets into reaction solution, promotes the circulation of reaction solution, and reaction solution circulates with the mode that also flows in reactor drum.Air is discharged from air (7).
The water valve of (10) feeds external jacket to water coolant from the cooling water inlet, and the flow velocity of water coolant is 1m/s, and water coolant is discharged from the water valve of cooling water outlet (11).
After reaction finished, reaction solution flowed out from material outlet (9), and the effusive speed of reaction solution is 1m/s.
The present invention successfully breaks away from mechanical stirring device through the global optimization and the appropriate design of reaction vessel and reaction process condition, has enlarged the useful volume of reaction vessel; Promoted the circulation of reaction solution; Significantly improve the catalytic efficiency (of enzyme, guaranteed higher transformation efficiency, the cool circulation water of suitable flow velocity is used in effective quickening of while inside reactor thermal cycling and the external jacket; Can control the temperature of reaction system easily and effectively; Energy consumption is few, and production cost is lower, is suitable for the production of mass-producing.From the present embodiment experiment effect, the productive rate of matter structure phosphatide reaches 67%, and under equal conditions the tank reactor productive rate for preparing matter structure phosphatide is merely 45%.
Present embodiment provides a germplasm structure phosphatide enzyme process reactor drum (1), and reactor drum (1) comprises retort (2) and external jacket (3).Retort (2) is cylindrical, top and bottom be semisphere, the height of retort (2) is 8m, diameter is 3m.Material inlet (4) internal diameter at retort (2) top is 20cm, and enzyme-added mouthful of (5) internal diameter at retort (2) top is 40cm, and air (7) internal diameter is 15cm.A gas inlet (6) is arranged at the bottom of retort (2); The internal diameter of gas inlet (6) is 10cm; Gas inlet (6) the onesize circular air-distributor (8) of internal diameter of ining succession, the internal diameter that distributing uniformly on the air-distributor (8) is the 3cm aperture, 200/m of aperture distribution density
2, bottom material outlet (9) internal diameter of retort (2) is 20cm.External jacket (3) is wrapped in the outside of retort (2), and the cooling water inlet (10) on the external jacket (3) is 10cm with cooling water outlet (11) internal diameter.
After soybean phospholipid and linolic acid mix by a certain percentage, get into retort (2) from material inlet (4), the speed of charging is 1m/s; 10% ratio in phosphatide quality in the mixture adds catalysis with enzyme (Novozym435 lypase) from enzyme-added mouthful (5) to retort
Cleaned air passes gets into retort (2) from gas inlet (6), and air velocity is 1m/s, becomes uniform bubble through air-distributor (8) and gets into reaction solution, promotes the circulation of reaction solution, and reaction solution circulates with the mode that also flows in reactor drum.Air is discharged from air (7).
The water valve of (10) feeds external jacket (3) to water coolant from the cooling water inlet, and the flow velocity of water coolant is 1m/s, and water coolant is discharged from the water valve of cooling water outlet (11).
After reaction finished, reaction solution flowed out from material outlet (9), and the effusive speed of reaction solution is 1m/s.
The present invention successfully breaks away from mechanical stirring device through the global optimization and the appropriate design of reaction vessel and reaction process condition, has enlarged the useful volume of reaction vessel, has promoted the circulation of reaction solution, significantly improves the catalytic efficiency (of enzyme, has guaranteed higher transformation efficiency.Suit in effective quickening of inside reactor thermal cycling simultaneously and the external jacket being used of cool circulation water of flow velocity can be controlled the temperature of reaction system easily and effectively, and energy consumption is few, and production cost is lower, is suitable for the production of mass-producing.From the present embodiment effect, the productive rate of present embodiment matter structure phosphatide reaches 68%, and under equal conditions the tank reactor productive rate for preparing matter structure phosphatide is merely 45%.
Present embodiment provides a germplasm structure phosphatide enzyme process reactor drum (1), and reactor drum (1) comprises retort (2) and external jacket (3).Retort (2) is cylindrical, top and bottom be semisphere, the height of retort (2) is 8m, diameter is 3m.Material inlet (4) internal diameter at retort (2) top is 15cm, and enzyme-added mouthful of (5) internal diameter at retort (2) top is 30cm, and air (7) internal diameter is 15cm.A gas inlet (6) is arranged at the bottom of retort (2); The internal diameter of gas inlet (6) is 10cm; Gas inlet (6) connects the onesize circular air-distributor (8) of internal diameter; The internal diameter that distributing uniformly on the air-distributor (8) is the 3cm aperture, and the aperture distribution density is 300/m
2, bottom material outlet (9) internal diameter of retort (2) is 20cm.External jacket (3) is wrapped in the outside of retort (2), and the cooling water inlet (10) on the external jacket (3) is 10cm with cooling water outlet (11) internal diameter.
After soybean phospholipid and linolic acid mix by a certain percentage, get into retort (2) from material inlet (4), the speed of charging is 1.5m/s; 20% ratio in phosphatide quality in the mixture adds catalysis with enzyme (Novozym435 lypase) from enzyme-added mouthful (5) to retort (2)
Cleaned air passes gets into retort (2) from gas inlet (6), and air velocity is 2m/s, becomes uniform bubble through air-distributor (8) and gets into reaction solution, promotes the circulation of reaction solution, and reaction solution circulates with the mode that also flows in reactor drum.Air is discharged from air (7).
The water valve of (10) feeds external jacket (3) to water coolant from the cooling water inlet, and the flow velocity of water coolant is 2m/s, and water coolant is discharged from the water valve of cooling water outlet (11).
After reaction finished, reaction solution flowed out from material outlet (9), and the effusive speed of reaction solution is 1.8m/s.
Based on present embodiment reactor drum and reaction method, the productive rate of matter structure phosphatide can reach 71%, and under equal conditions the tank reactor productive rate for preparing matter structure phosphatide is merely 48%.
Shown in accompanying drawing 1, the present invention is based on a kind of new enzyme process reactor drum (1), reactor drum (1) comprises retort (2) and external jacket (3).Retort (2) is cylindrical, top and bottom be semisphere, the height of retort (2) is 6m, diameter is 3m.Retort (2) top material inlet (4) internal diameter is 20cm, and vertical enzyme-added mouthful of (5) internal diameter of retort (2) is 40cm, and air (7) internal diameter is 18cm.A gas inlet (6) is arranged at the bottom of retort (2); The internal diameter of gas inlet (6) is 10cm; Gas inlet (6) the onesize circular air-distributor (8) of internal diameter of ining succession, the internal diameter that distributing uniformly on the air-distributor (8) is the 2cm aperture, 400/m of aperture distribution density
2Bottom material outlet (9) internal diameter of retort (2) is 25cm.External jacket (3) is wrapped in the outside of retort (2), and the cooling water inlet of (3) (10) are 12cm with cooling water outlet (11) internal diameter on the external jacket.
After soybean phospholipid and linolic acid mix by a certain percentage, get into retort (2) from material inlet (4), the speed of charging is 2m/s; 15% ratio in phosphatide quality in the mixture adds catalysis with enzyme (Novozym435 lypase) from enzyme-added mouthful in retort
Cleaned air passes gets into retort (2) from gas inlet (6), and air velocity is 3m/s, becomes uniform bubble through air-distributor (8) and gets into reaction solution, promotes the circulation of reaction solution, and reaction solution circulates with the mode that also flows in reactor drum.Air is discharged from air (7).
The water valve of (10) feeds external jacket (3) to water coolant from the cooling water inlet, and the flow velocity of water coolant is 3m/s, and water coolant is discharged from the water valve of cooling water outlet (11).
After reaction finished, reaction solution flowed out from material outlet (9), and the effusive speed of reaction solution is 2m/s.
Based on present embodiment reactor drum and reaction method, the productive rate of matter structure phosphatide can reach 74%, and under equal conditions the tank reactor productive rate for preparing matter structure phosphatide is merely 51%.
Shown in accompanying drawing 1, the present invention is based on a kind of new enzyme process reactor drum (1), reactor drum (1) comprises retort (2) and external jacket (3).Retort (2) is cylindrical, top and bottom be semisphere, the height of retort (2) is 6m, diameter is 3m.Retort (2) top material inlet (4) internal diameter is 15cm, and vertical enzyme-added mouthful of (5) internal diameter of retort (2) is 30cm, and air (7) internal diameter is 20cm.A gas inlet (6) is arranged at the bottom of retort (2); The internal diameter of gas inlet (6) is 8cm; Gas inlet (6) the onesize circular air-distributor (8) of internal diameter of ining succession, the internal diameter that distributing uniformly on the air-distributor (8) is the 3cm aperture, 240/m of aperture distribution density
2Bottom material outlet (9) internal diameter of retort (2) is 20cm.External jacket (3) is wrapped in the outside of retort (2), and the cooling water inlet of (3) (10) are 8cm with cooling water outlet (11) internal diameter on the external jacket.
After soybean phospholipid and linolic acid mix by a certain percentage, get into retort (2) from material inlet (4), the speed of charging is 1.5m/s; 15% ratio in phosphatide quality in the mixture adds catalysis with enzyme (Novozym435 lypase) from enzyme-added mouthful in retort
Cleaned air passes gets into retort (2) from gas inlet (6), and air velocity is 2.5m/s, becomes uniform bubble through air-distributor (8) and gets into reaction solution, promotes the circulation of reaction solution, and reaction solution circulates with the mode that also flows in reactor drum.Air is discharged from air (7).
The water valve of (10) feeds external jacket (3) to water coolant from the cooling water inlet, and the flow velocity of water coolant is 3m/s, and water coolant is discharged from the water valve of cooling water outlet (11).
After reaction finished, reaction solution flowed out from material outlet (9), and the effusive speed of reaction solution is 2m/s.
Present embodiment is further to the more excellent design of the carrying out of reaction vessel and reaction process condition, and based on present embodiment reactor drum and reaction method, the productive rate of matter structure phosphatide can reach 76%, and under equal conditions the tank reactor productive rate for preparing matter structure phosphatide is merely 51%.
In sum, the present invention purifies air through in reactor drum, feeding, and promotes the circulation of reaction solution; Improved the reaction efficiency of lypase; Accelerated the thermal cycling of inside reactor simultaneously, through with being used of cool circulation water, can effectively control the reaction temperature inside.In addition, reactor drum production cost provided by the invention is lower, and energy consumption is few, and useful volume is very big in the retort, is suitable for large-scale production, but also can be used as other reaction kettles or fermentor tank.
Claims (8)
1. matter structure phosphatide preparing process based on enzyme reactor is characterized in that may further comprise the steps:
(1) prepares matter structure phosphatide enzyme process reactor drum;
Said matter structure phosphatide enzyme process reactor drum comprises retort and external jacket; External jacket is wrapped in the outside of retort, and external jacket is provided with cooling water inlet and cooling water outlet, and water coolant gets into external jacket from the cooling water inlet, flows out from cooling water outlet behind the whole external jacket cooling reaction jar of flowing through;
Retort is provided with material inlet, is used for adding phosphatide and unsaturated fatty acids mixed solution to retort; Be provided with enzyme-added mouthful, katalaze(enzyme) gets into retort from enzyme-added mouthful; Be provided with gas inlet and air-distributor, purifying air gets into the air-distributor of being located at the retort bottom from gas inlet, and air-distributor is provided with some apertures, is that uniform small bubbles get into retort with air dispersion; Be provided with air, from then on air discharges in the retort; Be provided with material outlet, reaction solution is discharged from material outlet;
(2) after material proportionally mixes, get into retort from material inlet, the speed of material charging is 1～2m/s, then by 10～20% ratio of phosphatide quality in the mixture from enzyme-added mouthful to retort adding katalaze(enzyme);
(3) purify air from the gas inlet entering retort of reactor drum, air velocity is 1～3m/s, and purifying air becomes uniform bubble entering reaction solution from gas inlet through the air-distributor distribution, and unnecessary air is discharged from air;
(4) valve feeds external jacket to water coolant from the cooling water inlet, and the flow velocity of water coolant is 1～3m/s, and water coolant is discharged from cooling sea water outlet valve;
(5) after reaction finished, reaction solution flowed out from material outlet, and the effusive speed of reaction solution is 1～2m/s.
2. according to the said matter structure phosphatide preparing process of claim 1, it is characterized in that the speed of the said material charging of step (2) is 1.2～1.6m/s based on enzyme reactor.
3. according to the said matter structure phosphatide preparing process of claim 1, it is characterized in that the small aperture on the said air-distributor is 2～4cm based on enzyme reactor; Distribution density is 150～400/m
2
4. according to claim 1 or 3 said matter structure phosphatide preparing process, it is characterized in that the small aperture on the said air-distributor is 2.4～3.2cm based on enzyme reactor.
5. according to the said matter structure phosphatide preparing process of claim 1, it is characterized in that the said air velocity of step (3) is 1.5～2.5m/s based on enzyme reactor.
6. according to the said matter structure phosphatide preparing process of claim 1 based on enzyme reactor; The retort that it is characterized in that said matter structure phosphatide enzyme process reactor drum is middle for cylindrical; Top and bottom are semisphere; Air is located at the retort top, and enzyme-added mouth and material inlet are divided into the air both sides; Gas inlet is located at the bottom of retort, and gas inlet connects air-distributor, and gas inlet is identical with the air-distributor internal diameter, and air-distributor is circular, uniform distribution aperture on it, and the bottom of retort is provided with material outlet.
7. according to claim 1 or 6 said matter structure phosphatide preparing process based on enzyme reactor, it is characterized in that the height of the retort of said matter structure phosphatide enzyme process reactor drum is 5～10m, diameter is 2～4m; Retort top material inlet internal diameter is 10～20cm, and the vertical enzyme-added mouthful of internal diameter of retort is 20～40cm, and the air internal diameter is 12～18cm; The internal diameter of gas inlet is 5～10cm, and the bottom material outlet internal diameter of retort is 15～25cm; External jacket is wrapped in the outside of retort, and cooling water inlet on the external jacket and cooling water outlet internal diameter are 7～12cm; Small aperture on the said air-distributor is 2～4cm; Distribution density is 150～400/m
2
8. according to the said matter structure phosphatide preparing process based on enzyme reactor of claim 7, it is characterized in that the retort height of said matter structure phosphatide enzyme process reactor drum is 6m, diameter is 3m; Retort top material inlet internal diameter is 15cm, and the vertical enzyme-added mouthful of internal diameter of retort is 30cm, and the air internal diameter is 25cm; The internal diameter of gas inlet is 8cm, and the bottom material outlet internal diameter of retort is 20cm; External jacket is wrapped in the outside of retort, and cooling water inlet on the external jacket and cooling water outlet internal diameter are 8cm; The internal diameter of each aperture is 3cm on the air-distributor, and the density of little pore distribution is 240/m
2
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106834117A (en) * | 2015-10-27 | 2017-06-13 | 苏州迪欧益生物科技有限公司 | Enzyme reactor and intelligent enzyme reaction work station for releasing zooblast attaching |
| CN107551961A (en) * | 2017-11-03 | 2018-01-09 | 河北科技大学 | A kind of HTHP slurry bed reaction device |
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| CN101701229A (en) * | 2009-11-17 | 2010-05-05 | 广州海莎生物科技有限公司 | Method for preparing texture phospholipid and lecithin |
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| CN101701229A (en) * | 2009-11-17 | 2010-05-05 | 广州海莎生物科技有限公司 | Method for preparing texture phospholipid and lecithin |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106834117A (en) * | 2015-10-27 | 2017-06-13 | 苏州迪欧益生物科技有限公司 | Enzyme reactor and intelligent enzyme reaction work station for releasing zooblast attaching |
| CN107551961A (en) * | 2017-11-03 | 2018-01-09 | 河北科技大学 | A kind of HTHP slurry bed reaction device |
| CN107551961B (en) * | 2017-11-03 | 2022-10-25 | 河北科技大学 | High-temperature high-pressure slurry bed reaction device |
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| CN102827887B (en) | 2014-07-16 |
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