CN102827768A - System for researching of functions of neural circuits and regulation of animal behaviors and activities - Google Patents

System for researching of functions of neural circuits and regulation of animal behaviors and activities Download PDF

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CN102827768A
CN102827768A CN2012102906308A CN201210290630A CN102827768A CN 102827768 A CN102827768 A CN 102827768A CN 2012102906308 A CN2012102906308 A CN 2012102906308A CN 201210290630 A CN201210290630 A CN 201210290630A CN 102827768 A CN102827768 A CN 102827768A
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dichroscope
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CN102827768B (en
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曾绍群
刘亚丰
吕晓华
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Huazhong University of Science and Technology
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Abstract

The invention relates to fields like biology, medical science, neural science, etc. and discloses a system for researching of neural circuits and regulation of animal behaviors. According to the invention, visualization of cells and selection of cells of interests in a great perspective are realized; different patterns of rapid photostimulation on sample cells disposed on a fast high-precision two-dimensional electronic-control translation table are dynamically carried out; based on recorded electrophysiological signals or behavioral characteristics and the used stimulus patterns, the system is used for analyzing a selected specific neuronal projection relationship and for researching the processes of operation and integration of neurons, thereby further revealing the functions of a great specific neural circuit and regulating animal behaviors and activities.

Description

Neural circuit functionality of a kind of research and regulation and control animal behavior active system
Technical field
The present invention relates to fields such as biology, neuroscience, medical science, relate in particular to light genetics technology Application Areas.
Background technology
Human brain is made up of nearly hundred billion neurocyte; Form network through cynapse between the neurone, contact each other, transmission information; Form systems such as sensation, motion; Each system can be divided into the plurality of sub system again, and like vision, sense of smell, the sense of hearing, the sense of taste and body surface sense of touch etc. in the sensation, that the enforcement of its function depends on is dissimilar, be between the neural system different sites cell precisely contact and the neural loop that forms.Neural loop is the bridge between contact molecular cell function and the global behavior function; The research of the neural loop of specific function helps understanding the formation and the modification of neural loop; Information coding, processing and handle, with and and behavior between concern, thereby principle of work that can more profound understanding brain.
Neuromodulation is a kind of effective means of the neural loop of research, and it is a kind of neuromodulation technology of routine that medicine disturbs, but has the problem that the drug effect time is slow, spinoff is big; Electrode stimulating is an a kind of neuromodulation commonly used technology, but stimulated zone is big, lack of specific and spatial selectivity, has limited their application in neural loop research.The light genetics technology is a kind of neuromodulation technology of novelty, can can't harm, high-spatial and temporal resolution, both-way operation neurone, is fit to very much the neural circuit functionality of research, discloses getting in touch and mechanism of animal behavior activity and neural loop.
The light genetics technology is genetics technology and light stimulus technology bonded product.From genetics technology, developed multiple photaesthesia passage, can conveniently express on the culturing cell or in the living animal; From the light stimulus technology; There is limitation in current light stimulus method, as adopting the wide field stimulus modality, though can activate or suppress neurone; But it can activate whole animal sample or whole neural loop, can't realize that specific cells or the light stimulus of a group cell selective activate; As adopting based on scanning mirror, acousto-optic deflection device, light emitting diode matrix, spatial light modulator, liquid crystal or micro mirror technology; Though can realize the stimulation activating cells of high-spatial and temporal resolution; But must combine usually to be inverted or just to put on the fluorescent microscope; The light stimulus that only is applicable to culturing cell, brain section activates, and exists the light stimulus scope little, is not suitable for studying the behavioral activity of big neural network and regulation and control living animal.These have limited the application of light genetics technology in neural loop research.
Summary of the invention
Based on this, technical problem to be solved by this invention provides a kind of system of studying neural loop and regulation and control living animal behavioral activity, is used to study the behavioral activity of big specific function neural network and regulation and control living animal.
For solving the problems of the technologies described above; The present invention proposes a kind of system of studying neural loop and regulation and control living animal behavioral activity; It is characterized in that, comprise first laser apparatus, shutter, circular adjustable attenuator, coupled lens, single-mode fiber, imaging len, first dichroscope, prism, tube mirror, zooming system, object lens, the two-dimentional electronic control translation stage put successively; And imaging device CCD, computingmachine, interface circuit, shutter controller, electrophysiological recording system and translation stage unit;
The optical axis coincidence of said first laser apparatus, shutter, coupled lens, single-mode fiber, imaging len, single-mode fiber incident end face are positioned at coupled lens back focal plane place, and the single-mode fiber output end face is positioned at outside one times of focal length of imaging len; First dichroscope is placed with imaging len optical axis, tube mirror optical axis respectively in angle of 45 degrees; Said prism is between the tube mirror and first dichroscope; Said zooming system is installed between said tube mirror and the object lens, and said object lens are installed on said two-dimentional electronic control translation stage top; Said imaging device CCD is installed on first dichroscope top, and the light path of said imaging device CCD becomes miter angle with first dichroscope, and imaging device CCD and sample satisfy the imaging conjugate relation, is used for cell visual and cells of interest or regional choosing.
Said computingmachine connecting interface circuit, said interface circuit connects said shutter controller, electrophysiological recording system and translation stage unit respectively; Said shutter controller connects shutter, and said translation stage unit connects said two-dimentional electronic control translation stage; Said electrophysiological recording system connection electrodes.
Single-mode fiber outgoing end laser facula is positioned at a Jing Qianjiaopingmianchu through real image that imaging len becomes; Through tube mirror, zooming system, object lens; Obtain a laser facula ω ' relevant at object lens front focal plane place, relation below its size satisfies with the stereoscopic microscope magnification:
ω ′ = α M · β · ω 0
Wherein α is the vertical axle ratio of enlargement of imaging len, and M is the magnification of zooming system, and β is the magnification of object lens, ω 0Be single-mode fiber outgoing end laser spot diameter.
Further, the present invention also comprises eyepiece, and said eyepiece is positioned at the oblique upper of said prism group, and said prismatical position can translation, is used for the switching of sample reflected light between eyepiece and imaging device CCD.
Preferably, said circular adjustable attenuator adopts electro-optic crystal or acousto-optic modulator to replace, and said electro-optic crystal or acousto-optic modulator are connected computingmachine through corresponding electro-optic crystal unit with the acousto-optic modulator unit.
Said two-dimentional electronic control translation stage comprises grating chi, servomotor and feedback loop, to improve absolute fix precision and repetitive positioning accuracy.
Based on identical general technical design; For providing multi-wavelength's laser to carry out light stimulus; The invention allows for a kind of be used to study neural loop and regulation and control animal behavior active system, it is characterized in that: comprise first laser apparatus, acousto-optic tunable filter, coupled lens, single-mode fiber, imaging len, first dichroscope, tube mirror, zooming system, object lens, the two-dimentional electronic control translation stage put successively; And imaging device CCD, computingmachine, interface circuit, acousto-optic tunable filter unit, electrophysiological recording system and translation stage unit;
The optical axis coincidence of said first laser apparatus, coupled lens, single-mode fiber, imaging len, single-mode fiber incident end face are positioned at coupled lens back focal plane place, and the single-mode fiber output end face is positioned at outside one times of focal length of imaging len; First dichroscope is placed with imaging len optical axis, tube mirror optical axis respectively in angle of 45 degrees; Said prism is between the tube mirror and first dichroscope; Said zooming system is installed between said tube mirror and the object lens, and said object lens are installed on said two-dimentional electronic control translation stage top; Said imaging device CCD is installed on first dichroscope top, and the light path of said imaging device CCD becomes miter angle with first dichroscope, and imaging device CCD and sample satisfy the imaging conjugate relation.
Between said first laser apparatus and the acousto-optic tunable filter; Also place successively second laser apparatus, the 3rd laser apparatus ... N laser apparatus and corresponding respectively with it second dichroscope, the 3rd dichroscope .... N dichroscope; Said second dichroscope is placed with first laser optical axis, second laser optical axis respectively in angle of 45 degrees; The 3rd dichroscope is placed with first laser optical axis, the 3rd laser optical axis respectively in angle of 45 degrees; N dichroscope becomes 45 angles to place respectively with first laser optical axis, a N laser optical axis, acousto-optic tunable filter light hole center overlaps with first laser optical axis.
Said computingmachine connecting interface circuit, said interface circuit connects said acousto-optic tunable filter unit, electrophysiological recording system and translation stage unit respectively; Said acousto-optic tunable filter unit connects said acousto-optic tunable filter, and said translation stage unit connects said two-dimentional electronic control translation stage; Said electrophysiological recording system connection electrodes.Said computingmachine is chosen the optical maser wavelength that needs through interface circuit, acousto-optic tunable filter unit; And be coupled in the single-mode fiber through coupled lens; And then shine on the sample through object lens, can several microsecond speed switched laser wavelength with regulate the thorn laser power.
Can adopt the spectral filter swiveling wheel to replace acousto-optic tunable filter for reducing system cost, realize the optical maser wavelength switching.The spectral filter swiveling wheel be installed different spectral filters, by the circular wheel of electric machine control; Under computer control; Certain wavelength filter gets into first laser apparatus place optical axis, thus with spectral filter corresponding wavelength laser-transmitting, and other wavelength laser is reflected or absorb and can't pass through.
The present invention has realized the cell under the big visual field visual and cells of interest or regional choosing; Dynamically the animal sample cell that is positioned on the quick high accuracy two dimension electronic control translation stage is carried out the quick optical stimulation of different mode simultaneously; According to the electricity physiological signal of record or the stimulus modality of behavioural characteristic and use; Be used to analyze selected specific neurone projection relation; The computing of research neurone, integration process, and then disclose the neural loop function of big specific function, the activity of regulation and control animal behavior.
Description of drawings
Fig. 1 is the structural representation that is used to study neural circuit functionality and first embodiment of regulation and control animal behavior active system.
Fig. 2 is the structural representation that is used to study neural circuit functionality and second embodiment of regulation and control animal behavior active system.
Embodiment
Below through by instance the present invention being described in further detail, but following examples only are illustrative, and protection scope of the present invention does not receive the restriction of these embodiment.
, express on the cell in the animal brain cortex irritability photaesthesia albumen (like ChR2) or inhibition photaesthesia albumen (like NpHR) through genetics technology.Under blue light illumination, irritability photosensitive protein (like ChR2) makes the cationic channel on the cytolemma open like this, and positively charged ions such as the outer sodium of born of the same parents, calcium get into cell, and cell produces depolarize and excitement; In gold-tinted irradiation down, inhibition photosensitive protein (like NpHR) makes on the film chloride channel open, and the outer cl ions of born of the same parents gets into cell generation hyperpolarization, and cytoactive is suppressed.Different cells can the dissimilar photaesthesia passage of mark, and two kinds of dissimilar photaesthesia passages of perhaps same cell expressing just can activate these cells with the light stimulating method subsequently, and then the neural loop or the whole animal behavior of regulating and control to have specific function.
Activate the cell that these photaesthesia passages are expressed; Will realize that at first cell is visual, the light source of choosing suitable wavelength according to its spectral response curve again combines electrophysiological technique or study of behaviour register system to analyze neural circuit functionality with corresponding light stimulus pattern stimuli simultaneously; And then disclose the neural loop of big specific function with and animal behavior related; For realizing above target, need set up one and overlap independently system and corresponding method of operation, concrete technical scheme is following.
As shown in Figure 1; It is the structural representation of first embodiment of system; It comprises first laser apparatus 1, shutter 2, circular adjustable attenuator 3, coupled lens 4, single-mode fiber 5, dual-port module 6, prism 12, three order tubes 7, tube mirror 8, zooming system 9, object lens 10 and two-dimentional electronic control translation stage 11, and they are placed according to sequencing.The light path main body of tube mirror 8, zooming system 9, object lens 10 constituting body stereomicroscopes, wherein prism 12 is arranged in three order tubes 7 with eyepiece 13, and prism 12 can pull out, advance, and is used for the switching of sample reflected light between eyepiece and imaging device CCD.
First laser apparatus 1, shutter 2, coupled lens 4 optical axis centers overlap; The wavelength of first laser apparatus 1 should be chosen according to the curve of spectrum of expressed photaesthesia passage on the cell; Shutter 2 is controlled automatically through circuit and is opened or closed, and the collimation laser of opening then first laser apparatus, 1 output gets into circular adjustable attenuator 3, and circular adjustable attenuator 3 is coated with broadband dim light film; Be operated in visible light and near-infrared region, axially rotate circular adjustable attenuator 3 and can regulate laser output power continuously.Available electro-optic crystal or acousto-optic modulator replace circular adjustable attenuator, and laser power is regulated can pass through computer controlled automatic.Collimation laser is coupled lens 4 and converges in the single-mode fiber 5 through behind the circular adjustable attenuator 3.Single-mode fiber 5 incident end faces are positioned at the back focus place of coupled lens 4, and single-mode fiber 5 incident end face centers and coupled lens 4 optical axis coincidences.Laser with the single longitudinal mode transmission, shines freeboard at its output end face in single-mode fiber 5.At visible light wave range, the mode field diameter of single-mode fiber 5 is very little, common 3-9 μ m, and establishing the laser spot diameter of single-mode fiber 5 at the output end face place is ω 0
Dual-port module 6 bottoms are processed with the dovetail-indent of standard, can directly be fixed on the three order tubes 7, and eyepiece 13 is used for the eye-observation sample.Be processed with the standard C mouth on dual-port module 6 tops, corresponding imaging device CCD14 is installed; Be processed with interface in dual-port module 6 sides, fixedly single-mode fiber 5 and imaging len 15, imaging len 15 is incorporated into the laser of single-mode fiber output in the dual-port module 6; Portion is fixed with first dichroscope 16 within it; First dichroscope 16 is 45 degree with imaging len 15 optical axises, tube mirror 8 optical axises respectively and places; It is reflected in a mirror 8, zooming system 9, the object lens 10 place light paths first laser apparatus, 1 emitted laser, and the light transmission of other wave band.
There is a laser facula in single-mode fiber 5 output end faces; It is positioned at outside 15 1 times of focal lengths of imaging len; Theoretical according to lens imaging; Then the laser facula of single-mode fiber 5 output end faces will be a real image in the image space of imaging len 15, as size depend on that laser facula is to the distance of imaging len 15 and the focal length of imaging len.Move single-mode fiber 5 outgoing end faces and with the position of imaging len 15, make laser facula just be positioned at the back focal plane place of a mirror 8 through the picture of imaging len 15.According to optical principle; The first dichroscope laser light reflected is through becoming directional light after the tube mirror 8; Still be directional light through zooming system 9 again, object lens 10 converge to its place, focal plane to the incident directional light, as are fixed on the pallium place of animal on the two-dimentional electronic control translation stage 11.
The vertical axle ratio of enlargement that is set as as lens 15 is α, and the magnification of zooming system 9 is M, and the magnification of object lens 10 is β, and then the laser spot size ω ' of pallium place does
ω ′ = α M · β · ω 0
Regulate zooming system 9, perhaps change the object lens 10 of different multiples, can obtain laser facula, its size and the stereoscopic microscope magnification relation of being inversely proportional to of different size.Can reasonably choose the thorn laser spot according to cell size, the expression amount of photaesthesia albumen on cell like this, the light stimulus that obtains high spatial resolution activates.
System's first embodiment is when work; Open first laser apparatus 1; The animal of expressing the photaesthesia passage is placed on the two-dimentional electronic control translation stage 11; Observe the animal brain cortexes through eyepiece 13, treat that sample is clear in the visual field, electrode 17 is encapsulated on the target cell or the fixing animal of study of behaviour recording unit.Pull out prism 12 then, then eyepiece is unglazed passes through in the left side, can only pass through the right side eyepiece.Pallium is the light that sample sends, and behind object lens 10, zooming system 9, tube mirror 8, leads up to prism 12 entering right side eyepieces, and another road gets among the imaging device CCD14 through first dichroscope 16.CCD14 is positioned at the back focal plane place of a mirror 8, so object lens 10 focal planes and CCD14 photosensitive surface satisfy conjugate relation, not only can observation sample through CCD14, but also real time record sample.
Computingmachine 18 obtains the pallium images through interface circuit 19 control CCD14, chooses interested cell or zone and its positional information is converted into the positional information of two-dimentional electronic control translation stage 11.Computingmachine 18 is opened through interface circuit 19, shutter controller 20 control shutters 2 subsequently; Laser is through shutter 2, circular adjustable attenuator 3, coupled lens 4, single-mode fiber 5, first dichroscope 16, tube mirror 8, zooming system 9, object lens 10; On cells of interest or zone, form the laser facula that diameter is ω '; The time that stimulates was determined by the 2 open times of shutter; Regulate the laser power that circular adjustable attenuator 3 acquisitions need, activate power demand density with this cells of interest of quick active when surpassing the photaesthesia passage; Open the electrophysiological recording system corresponding electrical signal of 21 record object cells simultaneously or utilize the behavior of study of behaviour register system record animal to show.After first cells of interest stimulates; Shutter 2 is closed; Computingmachine 18 moves quickly into next cells of interest through interface circuit 19, the two-dimentional electronic control translation stage 11 of translation stage unit 22 controls; The open again laser power activating cells of regulating of shutter 2 then, and the like realize that the rapid stimulation of a plurality of cells in the visual field activates.The scope that stimulates depends on the stroke of two-dimentional electronic control translation stage 11, and stimulation rate depends primarily on the movement velocity of two-dimentional electronic control translation stage 11, and the precision of stimulation depends on CCD14 imaging precision, two-dimentional electronic control translation stage absolute fix precision and repetitive positioning accuracy thereof.The two dimension electronic control translation stage can add grating chi, servomotor and feedback loop to improve absolute fix precision and repetitive positioning accuracy.
The cell electrical signal that collects through interface circuit 19 according to computingmachine 18 or the movable information of study of behaviour signal and two-dimentional electronic control translation stage; Analyze, study the function of the neural loop of pallium and the behavioral activity of regulation and control animal, and then the announcement animal behavior relevant with specific neural loop is related.
Developed at present the photaesthesia albumen of multiple different excitation wavelengths, even system's first embodiment adopts a multiple-wavelength laser, received the restriction of ME, the laser of a lot of wavelength can't obtain.In addition, first embodiment adopts shutter 2 control light stimulus, and its mode of operation belongs to mechanical movement; Utmost dispatch only can reach several milliseconds; Therefore light stimulus speed is slow, can't be competent at the needs that quick optical stimulates, and needs to add other long wavelength laser in the system for this reason; And adopt other structure control light stimulus, be illustrated in figure 2 as the second embodiment structure iron.
Increase in the system second laser apparatus 22, the 3rd laser apparatus ... N laser apparatus 23, the second dichroscopes 24, the 3rd dichroscope ..., a N dichroscope 25, and acousto-optic tunable filter 26, they are placed successively.Second dichroscope 24 is placed with first laser apparatus, 1 optical axis, second laser apparatus, 2 optical axises respectively in angle of 45 degrees; The 3rd dichroscope is also placed with first laser apparatus, 1 optical axis, the 3rd laser optical axis respectively in angle of 45 degrees .... N dichroscope 25 placed with first laser apparatus 1, a N laser apparatus 23 optical axises respectively in angle of 45 degrees.Acousto-optic tunable filter 26 light hole centers and first laser apparatus, 1 optical axis coincidence.
During system works; Open all laser apparatus, computingmachine 18 applies the electrical signal of certain frequency, certain amplitude for acousto-optic tunable filter 26 through interface circuit, acousto-optic tunable filter unit 27; Then acousto-optic tunable filter 26 is chosen out with the wavelength corresponding with signal frequency; And with coupled lens 4 optical axis coincidences, converge in the single-mode fiber 5 through coupled lens 4, and then realize the light stimulus in cells of interest or zone through object lens.Change the signal amplitude of acousto-optic tunable filter, adjustable diffraction of light efficient then, thus realize the stimulation of the different luminous powers in sample place.In second embodiment; The electrical signal that is applied on the acousto-optic tunable filter 26 is only depended in the switching of optical maser wavelength and the adjusting of power; Usually only need several microseconds; So mechanical shutter in relative first embodiment, light stimulus speed can be brought up to 3 one magnitude, and therefore second embodiment can satisfy the quick optical stimulation activation of multiple photaesthesia protein expression cell.
Can adopt the spectral filter swiveling wheel to replace acousto-optic tunable filter 26 for reducing system cost, realize the optical maser wavelength switching.The spectral filter swiveling wheel be installed different spectral filters, by the circular wheel of electric machine control; Under computer control; Certain wavelength filter gets into first laser apparatus place optical axis; Thereby go out with loading spectral filter respective wavelength laser-transmitting, and other wavelength laser is reflected or absorb and can't pass through.This scheme can only be used for wavelength to be switched, and can't regulate laser power.
It should be noted last that; Above embodiment is only unrestricted in order to technical scheme of the present invention to be described; Although with reference to preferred embodiment the present invention is specified, those of ordinary skill in the art should be appreciated that and can make amendment or be equal to replacement technical scheme of the present invention; And not breaking away from the spirit and the scope of technical scheme of the present invention, it all should be encompassed in the middle of the claim scope of the present invention.

Claims (8)

1. study neural circuit functionality and regulation and control animal behavior active system for one kind; It is characterized in that, comprise first laser apparatus, shutter, circular adjustable attenuator, coupled lens, single-mode fiber, imaging len, first dichroscope, prism, tube mirror, zooming system, object lens, the two-dimentional electronic control translation stage put successively; And imaging device CCD, computingmachine, interface circuit, shutter controller, electrophysiological recording system and translation stage unit;
The optical axis coincidence of said first laser apparatus, shutter, coupled lens, single-mode fiber, imaging len, single-mode fiber incident end face are positioned at coupled lens back focal plane place, and the single-mode fiber output end face is positioned at outside one times of focal length of imaging len; First dichroscope is placed with imaging len optical axis, tube mirror optical axis respectively in angle of 45 degrees; Said prism is between the tube mirror and first dichroscope; Said zooming system is installed between said tube mirror and the object lens, and said object lens are installed on said two-dimentional electronic control translation stage top; Said imaging device CCD is installed on first dichroscope top, and the light path of said imaging device CCD becomes miter angle with first dichroscope, and imaging device CCD and sample satisfy the imaging conjugate relation;
Said computingmachine connecting interface circuit, said interface circuit connects said shutter controller, electrophysiological recording system and translation stage unit respectively; Said shutter controller connects shutter, and said translation stage unit connects said two-dimentional electronic control translation stage, said electrophysiological recording system connection electrodes.
2. neural circuit functionality of research according to claim 1 and regulation and control animal behavior active system, relation below its thorn laser spot satisfies:
ω ′ = α M · β · ω 0
Wherein α is the vertical axle ratio of enlargement of imaging len, and M is the magnification of zooming system, and β is the magnification of object lens, ω 0Be single-mode fiber outgoing end face laser spot diameter.
3. neural circuit functionality of research according to claim 1 and regulation and control animal behavior active system; Also comprise eyepiece; Said eyepiece is positioned at the oblique upper of said prism group, and said prismatical position can translation, is used for the switching of sample reflected light between eyepiece and imaging device CCD.
4. according to neural circuit functionality of the described research of one of claim 1-3 and regulation and control animal behavior active system; It is characterized in that; Said circular adjustable attenuator adopts electro-optic crystal or acousto-optic modulator to replace, and said electro-optic crystal, acousto-optic modulator connect computingmachine through electro-optic crystal unit, acousto-optic modulator unit respectively.
5. neural circuit functionality of research according to claim 4 and regulation and control animal behavior active system is characterized in that said two-dimentional electronic control translation stage comprises grating chi, servomotor and feedback loop.
6. study neural loop and regulation and control animal behavior active system for one kind, it is characterized in that: comprise first laser apparatus, acousto-optic tunable filter, coupled lens, single-mode fiber, imaging len, first dichroscope, tube mirror, zooming system, object lens, the two-dimentional electronic control translation stage put successively; And imaging device CCD, computingmachine, interface circuit, acousto-optic tunable filter unit, electrophysiological recording system and translation stage unit;
The optical axis coincidence of said first laser apparatus, coupled lens, single-mode fiber, imaging len, single-mode fiber incident end face are positioned at coupled lens back focal plane place, and the single-mode fiber output end face is positioned at outside one times of focal length of imaging len; First dichroscope is placed with imaging len optical axis, tube mirror optical axis respectively in angle of 45 degrees; Said prism is between the tube mirror and first dichroscope; Said zooming system is installed between said tube mirror and the object lens, and said object lens are installed on said two-dimentional electronic control translation stage top; Said imaging device CCD is installed on first dichroscope top, and the light path of said imaging device CCD becomes miter angle with first dichroscope, and imaging device CCD and sample satisfy the imaging conjugate relation;
Said computingmachine connecting interface circuit, said interface circuit connects said acousto-optic tunable filter unit, electrophysiological recording system and translation stage unit respectively; Said acousto-optic tunable filter unit connects said acousto-optic tunable filter, and said translation stage unit connects said two-dimentional electronic control translation stage, said electrophysiological recording system connection electrodes;
Between said first laser apparatus and the acousto-optic tunable filter; Also place successively second laser apparatus, the 3rd laser apparatus ... N laser apparatus and corresponding respectively with it second dichroscope, the 3rd dichroscope .... N dichroscope; Said second dichroscope is placed with first laser optical axis, second laser optical axis respectively in angle of 45 degrees; The 3rd dichroscope is placed with first laser optical axis, the 3rd laser optical axis respectively in angle of 45 degrees; N dichroscope becomes 45 angles to place respectively with first laser optical axis, a N laser optical axis, acousto-optic tunable filter light hole center overlaps with first laser optical axis.
Said computingmachine is chosen the optical maser wavelength that needs through interface circuit, acousto-optic tunable filter unit, and is coupled in the single-mode fiber through coupled lens, and then is coupled to and carries out light stimulus in the object lens.
7. neural circuit functionality of research according to claim 6 and regulation and control animal behavior active system; It is characterized in that; Adopt the spectral filter swiveling wheel to replace said acousto-optic tunable filter and acousto-optic tunable filter unit; Said spectral filter swiveling wheel connects said computingmachine, under the control of computingmachine, realizes the optical maser wavelength switching.
8. according to claim 6 or neural circuit functionality of 7 described researchs and regulation and control animal behavior active system, it is characterized in that said two-dimentional electronic control translation stage comprises grating chi, servomotor and feedback loop.
CN 201210290630 2012-08-15 2012-08-15 System for researching of functions of neural circuits and regulation of animal behaviors and activities Expired - Fee Related CN102827768B (en)

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