CN102823403A - Breeding method of pteroceltis tatarinowii polyploid - Google Patents

Abstract

The invention discloses a breeding method of a pteroceltis tatarinowii polyploid, which is featured with simplicity, easiness and obvious mutagenizing effect. The breeding method comprises the following steps of: carrying out liquid dropping mutagenic treatment on a growth point of a pteroceltis tatarinowii seedling by using a colchicine solution with concentration of 0.4-0.8%; setting corresponding moisturizing and shading for the growth point of the pteroceltis tatarinowii seedling; carrying out mutagenic treatment on the pteroceltis tatarinowii seedling within a preset time; and removing an effect of a liquid medicine in time, wherein an obtained polyploid has the characteristics of thickened plant leaves, darkened leaf color, thickened stems, increased pitches, enlarged air holes, reduced number of the air holes per unit leaf area, obviously increased chloroplast number of a guard cell and plant mutation rate of more than 30%; and a basis is laid for obtaining a new germplasm of a pteroceltis tatarinowii tree tetraploid.

Description

A kind of breeding method of wingceltis polyploid

Technical field

The present invention relates to the breeding field of plant polyploid, specifically a kind of breeding method of wingceltis polyploid.

Background technology

Wingceltis (Pteroceltis tatarinowii) is Chinese distinctive autogenus plant, is the pioneer tree species of Limestone Mountain Areas afforestation, and is drought-resistant barren, well developed root system, and the stretching, extension of spiraling between the rock slot of being everlasting, sprout tillers property is strong, and the life-span is long.China did not carry out system research to wingceltis seeds resource so far, still was in the low conventional breeding stage of utilizing level, and artificial induction's polyploid is one of important channel that obtains plant newtype or new germ plasm.

Summary of the invention

For solving the technical problem of above-mentioned existence, the invention provides a kind of breeding method of wingceltis polyploid, adopting concentration is its growing point of colchicine solution processing of 0.4-0.8%, processing ease, stem apex mutagenic fungi rate is high, and mutagenesis is effective.

For achieving the above object, the technical scheme that the present invention adopts is following:

A kind of breeding method of wingceltis polyploid, realize through following steps:

1) the wingceltis seed of will be by the end of October collecting at the beginning of 11 months sprouts the back sowing in coil in the cave through husky the Tibetan, after emerging by Routine Management;

2) early April comes up, and when cotyledon flattens and to treat that true leaf is sprouted, is that the rayon balls of the colchicine solution of 0.4-0.8% is placed on the shoot tip meristem of seedling with dipping in concentration; The dropping liquid that carries out in 72 hours is handled, every day sooner or later each once, 1-3 drips at every turn; Cover plastic film and sunshade net then,, after the scheduled time, remove rayon balls since the dropping liquid calculating treatmenting time first time; And use flushing with clean water, remove the soup effect;

3) seedling is through mutagenic treatment after month, and the growth of seedling of survival is accelerated, to August the survive polyploid The Characters of plant obvious, and confirm through microscopy.

The method of wingceltis polyploid of inducing provided by the invention is simple, induces effect remarkable, adopts the colchicine solution of 0.4-0.8% concentration that wingceltis growth of seedlings point is carried out the drip method mutagenic treatment; And preserve moisture and the shading setting effect of in the given time the wingceltis seedling being carried out mutagenic treatment and in time removing soup, the polyploid plant blade thickening of formation accordingly; The leaf look deepens, and it is big that stem chap and pitch become, and pore increases; The unit leaf area stoma number reduces; Guard cell's chloroplast is counted showed increased, and the variant rate surpasses 30%, lays a good foundation for obtaining wingceltis tree tetraploid new germ plasm.

Embodiment

Describe the present invention below in conjunction with specific embodiment:

1) the full wingceltis seed of will be by the end of October collecting at the beginning of 11 months is hidden through sand and to be sprouted the back sowing in the dish of cave, manages routinely after emerging;

2) early April comes up, and when cotyledon flattens and to treat that true leaf is sprouted, is that the rayon balls of the colchicine solution of 0.2%-1.2% is placed on the shoot tip meristem of seedling with dipping in concentration; The dropping liquid that carries out in 72 hours is handled, every day sooner or later each once, 1-3 drips at every turn; Cover plastic film and sunshade net then, realize preserving moisture and preventing high light calcination seedling, since the dropping liquid calculating treatmenting time first time; After the scheduled time, remove rayon balls, and use flushing with clean water, remove the soup effect;

3) seedling is through after the mutagenic treatment; Growth is suppressed; The part cotyledon is loose, dark green, the stem apex poor growth, after one month, accelerates through the growth of seedling of mutagenic treatment and survival; To August investigation statistics is carried out in the shape performance of survival plant; Obviously become big to handle the strain blade, broaden, thickening, turn green, crumple, blade sawtooth and the unusual plant of the outer shape performance object of observation of attaching most importance to such as deepen, simultaneously the plant that blade lower epidermis guard cell becomes greatly, the minimizing of unit are stomatal number, guard cell's chloroplast number increase is labeled as special strain, concentration is the plant that the colchicine solution of 0.4%-0.8% was handled; Aberration rate surpasses 30%, adds their confirmation through microscopy at last.

From table, can find out that see from the mutagenesis effect, 0.2%, 0.4%, 0.6%, 0.8%, 1.2% colchicine is all effective to inducing of wingceltis seedling apical point.Show from lethality, along with colchicine concentration raises and the processing time prolongation, the plant lethality is in rising trend, and colchicine concentration is 1.2%, and the young plant lethality of processing time 72h is the highest, reaches 24.2%.Show that from the plant aberration rate in 12 treatment combinations, the variant rate with 0.8% colchicine concentration processing 72h is the highest especially; Reach more than 34.2%; Being the optimization process combination, secondly is to handle 72h with 0.6% colchicine concentration, and the variant rate is 33.3%; Be to handle 72h with 0.4% colchicine concentration once more, the variant rate is 32.5%.

Certainly, above-mentioned explanation is not to be limitation of the present invention, and the present invention also is not limited in above-mentioned giving an example, and variation, remodeling, interpolation or replacement that the technical staff in present technique field is made in essential scope of the present invention also should belong to protection scope of the present invention.

Claims (1)

1. the breeding method of a wingceltis polyploid is characterized in that, realizes through following steps:

1) the wingceltis seed of will be by the end of October collecting at the beginning of 11 months sprouts the back sowing in coil in the cave through husky the Tibetan, after emerging by Routine Management;

2) early April comes up, and when cotyledon flattens and to treat that true leaf is sprouted, is that the rayon balls of the colchicine solution of 0.4-0.8% is placed on the shoot tip meristem of seedling with dipping in concentration; The dropping liquid that carries out in 72 hours is handled, every day sooner or later each once, 1-3 drips at every turn; Cover plastic film and sunshade net then,, after the scheduled time, remove rayon balls since the dropping liquid calculating treatmenting time first time; And use flushing with clean water, remove the soup effect;

3) seedling is through mutagenic treatment after month, and the growth of seedling of survival is accelerated, to August the survive polyploid The Characters of plant obvious, and confirm through microscopy.