CN102818740B - Identification method of resistance to aspergillus flavus infection of peanuts and application thereof - Google Patents
Identification method of resistance to aspergillus flavus infection of peanuts and application thereof Download PDFInfo
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- CN102818740B CN102818740B CN201210332642.2A CN201210332642A CN102818740B CN 102818740 B CN102818740 B CN 102818740B CN 201210332642 A CN201210332642 A CN 201210332642A CN 102818740 B CN102818740 B CN 102818740B
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Abstract
The invention discloses an identification method of resistance to aspergillus flavus infection of peanuts and an application thereof. The identification method disclosed by the invention comprises the following steps: inoculating an aspergillus flavus strain into peanut seeds, further performing dark culture, then adopting a measuring instrument, namely an analytical balance, weighing the seeds after dark culture, finally calculating an aspergillus flavus infection index and performing resistance evaluation. According to the identification method disclosed by the invention, the resistance to the aspergillus flavus infection of a peanut variety can be effectively identified under relatively simple experimental conditions, and the identification method is particularly suitable for batch screening of peanut breeding materials, and has the advantages of simple operation, low resistance identification cost, accurate experimental results and high precision.
Description
Technical field
The invention belongs to agricultural technology field, particularly authentication method and an application thereof of cultivating peanut the resistance that aspergillus flavus is infected.
Background technology
The resistance that peanut infects aspergillus flavus refers to that Kernel of Peanut, after being subject to having and producing the aspergillus flavus (Aspergillus flavus) of aflatoxin ability and aspergillus parasiticus (A.parasiticus) and infect, can suppress the characteristic of above-mentioned fungi generation aspergillus flavus.Have and can significantly reduce the aspergillus flavus level of pollution in peanut products to the peanut varieties of aspergillus flavus infection resistance.The method of the resistance that evaluation peanut infects aspergillus flavus is at present mainly the content by detecting aspergillus flavus after artificial infection, its detection method is thin-layered chromatography and high performance liquid chromatography, its complicated operation, sample detection expense are high, high to equipment requirement, can not effectively be applied to the Resistance Identification in peanut breeding research.Also have and estimate that by detecting by an unaided eye after artificial infection aspergillus flavus infects quantity and the degree of kind of benevolence, although simple to operate, because vision observation differs greatly, its resultant error is large, the resistance can not effectively evaluating peanut aspergillus flavus being infected.
Summary of the invention
For the shortcoming that overcomes above-mentioned prior art is with not enough, primary and foremost purpose of the present invention is to provide an authentication method of cultivating peanut the resistance that aspergillus flavus is infected.
Another object of the present invention is to provide the application of the authentication method of the resistance that described peanut infects aspergillus flavus.
Object of the present invention is achieved through the following technical solutions: the authentication method of the resistance that cultivates peanut infects aspergillus flavus, comprises the following steps: (1) selects the strong aspergillus flavus strain of infection ability; (2) selection of peanut seed, sterilization and rehydration; (3) with yellow inulinase inoculation peanut seed; (4) secretly cultivate; (5) seed after secretly cultivating is weighed; (6) calculate infection by Aspergillus flavus index; (7) evaluation of resistance;
Preferably, the authentication method of the resistance that cultivates peanut infects aspergillus flavus, comprises the following steps:
(1) select the strong aspergillus flavus strain of infection ability to cultivate, when inoculation, be mixed with aspergillus spore suspending liquid for subsequent use;
(2) select healthy peanut seed, peel off pericarp, get seed that seed coat is intact as sample, be sub-packed in numbered double dish, clean with distilled water flushing after peanut seed being sterilized with captan, making the water cut after seed rehydration is 15%～25%, weigh respectively, in double dish the weight of sample seed be designated as Wi(as W01, W02 ...); Select the peanut seed of high sense kind in contrast, be contained in numbered double dish, clean with distilled water flushing after with captan, peanut seed being sterilized, making the water cut after seed rehydration is 15%～25%, weigh, the weight that contrasts seed in double dish is designated as WCK;
(3) the aspergillus spore suspending liquid of being prepared by step (1) adds in step (2) and is equipped with in the double dish of sample, rotates double dish and makes spore evenly be attached to the surface of the seed; The same sample of operation of contrast, what difference was only to add is sterilized water;
(4) double dish being placed in to incubator secretly cultivates;
(5) by secretly cultivate after seed weigh, the weight of sample seed be designated as Wif(as W01f, W02f ...), the weight of contrast seed is designated as WCKf;
(6) infection by Aspergillus flavus index (F) calculates by following formula:
The definitely Fi=of infestation index (Wif-Wi)/Wi
The Fi=[(Wif-Wi of infestation index relatively)/Wi]/[(WCKf-WCK)/WCK];
(7) evaluation of resistance: F 0～0.150 be high resistance (HR), 0.151～0.300 be in anti-(MR), 0.301～0.500 be middle sense (MS), 0.501～1.000 be that height is felt (HS);
The strong aspergillus flavus strain of infection ability described in step (1) is preferably aspergillus flavus strain As33.2890;
Cultivation described in step (1) preferably in Czapek's medium, 28 ℃ of dark culturing;
The preparation of the aspergillus spore suspending liquid described in step (1) preferably adopts following methods to prepare: when inoculation, collect and cultivate the spore of 7～8 days with sterilized water, sterilized water is resuspended, and adjusts spore concentration and make to contain 1 × 10 in every ml water
8individual spore, to obtain final product;
The amount of the packing described in step (2) is preferably 30～50 of each double dish;
Height sense kind described in step (2) is preferably Shanyou 523;
The addition of the aspergillus spore suspending liquid described in step (3) is preferably each double dish and adds 1ml;
The temperature of the dark cultivation described in step (4) is preferably 25～32 ℃, and the time is preferably 8 days.
The authentication method of the resistance that described peanut infects aspergillus flavus can be applicable to the Resistance Identification in peanut breeding research, is especially applicable to being applied to peanut breeding material is done to screening in batches.
The present invention has following advantage and effect with respect to prior art:
The present invention adopts gauging instrument analytical balance, detect by the method for weighing the degree that aspergillus flavus is infected, can under comparatively simple experiment condition, effectively identify the resistance that peanut varieties infects aspergillus flavus, especially be applicable to peanut breeding material to do screening in batches, simple to operate, Resistance Identification cost is low, and experimental result degree of accuracy is high.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited to this.
Embodiment 1
(1) take the strong aspergillus flavus strain As33.2890(Institute of Microorganism, Academia Sinica of infecting potential) as inoculation bacterial strain; Aspergillus flavus strain As33.2890 is placed in to Czapek's medium, 28 ℃ of dark culturing, when inoculation, collects with sterilized water the spore of cultivating 7 days, sterilized water is resuspended, adjusts spore concentration and makes to contain 1 × 10 in every ml water
8individual spore, for subsequent use as aspergillus spore suspending liquid;
(2) select three, peanut varieties Taishan meat (crop investigations institute of academy of agricultural sciences of Guangdong Province) seed, select healthy peanut seed, peel off pericarp, choose seed that seed coat is intact as sample, in 3 numbered double dish of packing, 40 seeds of each double dish, clean with distilled water flushing after peanut seed being sterilized with captan, making seed moisture content is 20%, weighs respectively, and in double dish, the weight of sample seed is designated as W01=22.1203g, W02=22.2315g, W03=22.2543g; Select 40 seeds of high sense kind Shanyou 523 (Shantou City, Guangdong Province Institute of agricultural sciences) in contrast, be contained in a numbered double dish, weigh, the weight that contrasts seed in double dish is designated as WCK=28.6400g(in table 1); In the sterile petri dish that peanut seed is arranged in;
(3) the aspergillus spore suspending liquid that adds 1ml step (1) to prepare toward each double dish that sample is housed in step (2), rotates double dish and makes spore evenly be attached to the surface of the seed; The same sample of operation of contrast, what difference was only to add is sterilized water;
(4) double dish is placed in to incubator, 25 ℃ of dark cultivations 8 days;
(5) seed after secretly cultivating is weighed, the weight of sample seed is designated as respectively W01f=25.8807g, W02f=28.4786g, W03f=25.7259g(in table 1), the weight of contrast seed is designated as to WCKf, WCKf=58.2824g, corresponding WCK=28.6400g;
(6) calculating of infection by Aspergillus flavus index (F):
As sample 01, Wi=W01=22.1203g, W01f=25.8807g, WCK=28.6400g, WCKf=58.2824g, substitution formula calculates respectively absolute infestation index and relative infestation index:
The absolute F01=of infestation index (W01f-W01)/W01=0.1700,
The F01=[(W01f-W01 of infestation index relatively)/W01]/[(WCKf-WCK)/WCK]=0.1643,
The rest may be inferred, calculates infestation index's (in table 1) of sample 02,03;
Evaluation of resistance: the absolute infestation index of three the meat kinds in the Taishan and relatively infestation index are all between 0.151～0.300, in belonging to anti-(HR).
The Resistance Identification result of three the meat kinds in table 1 Taishan
Embodiment 2
(1) take the strong aspergillus flavus strain As33.2890(Institute of Microorganism, Academia Sinica of infecting potential) as inoculation bacterial strain; Aspergillus flavus strain As33.2890 is placed in to Czapek's medium, 28 ℃ of dark culturing, when inoculation, collects with sterilized water the spore of cultivating 7 days, sterilized water is resuspended, adjusts spore concentration and makes to contain 1 × 10 in every ml water
8individual spore, for subsequent use as aspergillus spore suspending liquid;
(2) select academy of agricultural sciences's crop investigations institute of peanut varieties Zhan Qiu 48(Guangdong Province) seed, select healthy peanut seed, peel off pericarp, choose seed that seed coat is intact as sample, in 3 numbered double dish of packing, 30 seeds of each double dish, clean with distilled water flushing after peanut seed being sterilized with captan, making seed moisture content is 20%, weighs respectively, and in double dish, the weight of sample seed is designated as W01=18.3600g, W02=18.4800g, W03=18.6000g; Select 30 seeds of high sense kind Shanyou 523 (Shantou City, Guangdong Province Institute of agricultural sciences) in contrast, be contained in a numbered double dish, clean with distilled water flushing after peanut seed being sterilized with captan, making seed moisture content is 20%, weigh, the weight that contrasts seed in double dish is designated as WCK=19.1400g(in table 2);
(3) the aspergillus spore suspending liquid that adds 1ml step (1) to prepare toward each double dish that sample is housed in step (2), rotates double dish and makes spore evenly be attached to the surface of the seed; The same sample of operation of contrast, what difference was only to add is sterilized water;
(4) double dish is placed in to incubator, 32 ℃ of dark cultivations 8 days;
(5) seed after secretly cultivating is weighed, the weight of sample seed is designated as respectively W01f=24.5767g, W02f=26.4190g, W03f=27.8126g(in table 2), the weight of contrast seed is designated as WCKf, WCKf=38.5547g, corresponding WCK=19.1400g;
(6) calculate according to infection by Aspergillus flavus index (F) formula:
Sample 01:W01=18.3600g, W01f=24.5767g, WCK=19.1400g, WCKf=38.5547g, substitution formula calculates respectively absolute infestation index and relative infestation index:
The absolute F01=of infestation index (W01f-W01)/W01=0.3386,
The F01=[(W01f-W01 of infestation index relatively)/W01]/[(WCKf-WCK)/WCK]=0.3338,
The rest may be inferred, calculates infestation index's (in table 2) of sample 02,03;
Evaluation of resistance: the infestation index in profound autumn 48, between 0.301～0.500, belongs to middle sense (MS).
The Resistance Identification result in profound autumn 48 of table 2
Embodiment 3
(1) take the strong aspergillus flavus strain As33.2890(Institute of Microorganism, Academia Sinica of infecting potential) as inoculation bacterial strain; Aspergillus flavus strain As33.2890 is placed in to Czapek's medium, 28 ℃ of dark culturing, when inoculation, collects with sterilized water the spore of cultivating 7 days, sterilized water is resuspended, adjusts spore concentration and makes to contain 1 × 10 in every ml water
8individual spore, for subsequent use as aspergillus spore suspending liquid;
(2) select academy of agricultural sciences's crop investigations institute of peanut varieties Yue You 116(Guangdong Province) seed, select healthy peanut seed, peel off pericarp, choose seed that seed coat is intact as sample, in 3 numbered double dish of packing, 50 seeds of each double dish, clean with distilled water flushing after peanut seed being sterilized with captan, making seed moisture content is 20%, weighs respectively, and in double dish, the weight of sample seed is designated as W01=31.0514g, W02=33.2279g, W03=33.1050g; Select 50 seeds of high sense kind Shanyou 523 (Shantou City, Guangdong Province Institute of agricultural sciences provides) in contrast, be contained in a numbered double dish, clean with distilled water flushing after peanut seed being sterilized with captan, making seed moisture content is 20%, weigh, the weight that contrasts seed in double dish is designated as WCK=34.9150g(in table 3);
(3) the aspergillus spore suspending liquid that adds 1ml step (1) to prepare toward each double dish that sample is housed in step (2), rotates double dish and makes spore evenly be attached to the surface of the seed; The same sample of operation of contrast, what difference was only to add is sterilized water;
(4) double dish is placed in to incubator, 30 ℃ of dark cultivations 8 days;
(5) seed after secretly cultivating is weighed, the weight of sample seed is designated as respectively W01f=60.9818g, W02f=64.8044g, W03f=55.3185g(in table 3), the weight that infects the most serious CK seed of aspergillus flavus in contrast is designated as WCKf, WCKf=69.2364g, corresponding WCK=34.9150g;
(6) calculate according to infection by Aspergillus flavus index (F) formula:
Sample 01:W01=31.0514g, W01f=60.9818g, WCK=34.9150g, WCKf=69.2364g, substitution formula calculates respectively absolute infestation index and relative infestation index:
The absolute F01=of infestation index (W01f-W01)/W01=0.9639,
The F01=[(W01f-W01 of infestation index relatively)/W01]/[(WCKf-WCK)/WCK]=0.9806,
The rest may be inferred, calculates infestation index's (in table 3) of sample 02,03;
Evaluation of resistance: the infestation index of Guangdong oil 116, between 0.501～1.000, belongs to high sense (HS).
The Resistance Identification result of table 3 Guangdong oil 116
Above-described embodiment is preferably embodiment of the present invention; but embodiments of the present invention are not restricted to the described embodiments; other any do not deviate from change, the modification done under Spirit Essence of the present invention and principle, substitutes, combination, simplify; all should be equivalent substitute mode, within being included in protection scope of the present invention.
Claims (10)
1. an authentication method of cultivating peanut the resistance that aspergillus flavus is infected, is characterized in that comprising the following steps:
(1) select the strong aspergillus flavus strain of infection ability to cultivate, when inoculation, be mixed with aspergillus spore suspending liquid for subsequent use;
(2) select healthy peanut seed, peel off pericarp, get seed that seed coat is intact as sample, be sub-packed in numbered double dish, clean with distilled water flushing after peanut seed being sterilized with captan, making the water cut after seed rehydration is 15%～25%, weighs respectively, and in double dish, the weight of sample seed is designated as Wi; Select the peanut seed of high sense kind in contrast, be contained in numbered double dish, clean with distilled water flushing after with captan, peanut seed being sterilized, making the water cut after seed rehydration is 15%～25%, weigh, the weight that contrasts seed in double dish is designated as WCK;
(3) the aspergillus spore suspending liquid of being prepared by step (1) adds in step (2) and is equipped with in the double dish of sample, rotates double dish and makes spore evenly be attached to the surface of the seed; The same sample of operation of contrast, what difference was only to add is sterilized water;
(4) double dish being placed in to incubator secretly cultivates;
(5) seed after secretly cultivating is weighed, the weight of sample seed is designated as Wif, the weight of contrast seed
Be designated as WCKf;
(6) infection by Aspergillus flavus index F calculates by following formula:
The definitely Fi=of infestation index (Wif-Wi)/Wi,
The Fi=[(Wif-Wi of infestation index relatively)/Wi]/[(WCKf-WCK)/WCK].
2. the authentication method of the resistance that peanut according to claim 1 infects aspergillus flavus, is characterized in that: the aspergillus flavus strain that the infection ability described in step (1) is strong is aspergillus flavus strain As33.2890.
3. the authentication method of the resistance that peanut according to claim 1 infects aspergillus flavus, is characterized in that: the cultivation described in step (1) in Czapek's medium, 28 ℃ of dark culturing.
4. the authentication method of the resistance that peanut according to claim 1 infects aspergillus flavus, it is characterized in that: the preparation of the aspergillus spore suspending liquid described in step (1) adopts following methods to prepare: when inoculation, collect the spore of cultivating 7～8 days with sterilized water, sterilized water is resuspended, and adjusts spore concentration and make to contain 1 × 10 in every ml water
8individual spore, to obtain final product.
5. the authentication method of the resistance that peanut according to claim 1 infects aspergillus flavus, is characterized in that: the amount of the packing described in step (2) is 30～50 of each double dish.
6. the authentication method of the resistance that peanut according to claim 1 infects aspergillus flavus, is characterized in that: the height sense kind described in step (2) is Shanyou 523.
7. the authentication method of the resistance that peanut according to claim 1 infects aspergillus flavus, is characterized in that: the addition of the aspergillus spore suspending liquid described in step (3) is that each double dish adds 1ml.
8. the authentication method of the resistance that peanut according to claim 1 infects aspergillus flavus, is characterized in that: the temperature of the dark cultivation described in step (4) is 25～32 ℃, and the time is 8 days.
9. the application of the authentication method of the resistance that the peanut described in claim 1～8 any one infects aspergillus flavus, is characterized in that: the authentication method of the resistance that described peanut infects aspergillus flavus is applied in the Resistance Identification of peanut breeding research.
10. the application of the authentication method of the resistance that peanut according to claim 9 infects aspergillus flavus, is characterized in that: the authentication method of the resistance that described peanut infects aspergillus flavus is applied in peanut breeding material work is screened in batches.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103114124B (en) * | 2013-02-06 | 2015-09-09 | 青岛农业大学 | One cultivates peanut infects the method for aflatoxin |
| CN103509723B (en) * | 2013-09-25 | 2015-07-01 | 中国农业科学院农产品加工研究所 | Aspergillus flavus producing no aflatoxin and application thereof |
| CN105340603B (en) * | 2015-11-09 | 2018-06-22 | 山东省花生研究所 | A kind of method of the aspergillus flavus Field infection of peanut seed |
| CN105230376A (en) * | 2015-11-12 | 2016-01-13 | 青岛友诚高新技术有限公司 | Method of field infected peanuts |
| CN106119337A (en) * | 2016-06-27 | 2016-11-16 | 山东省农业科学院生物技术研究中心 | A kind of Rapid identification peanut varieties method to Aspergillus flavus resistance |
| CN106596492A (en) * | 2016-12-28 | 2017-04-26 | 商丘市农林科学院 | Method for identifying infection resistance of peanuts to aspergillus flavus and application thereof |
| CN109001385A (en) * | 2018-06-15 | 2018-12-14 | 中国农业科学院油料作物研究所 | The indoor appraising method and its application of a kind of peanut pod to Aspergillus flavus infection resistance |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4181853A (en) * | 1976-12-10 | 1980-01-01 | Varian Associates, Inc. | Liquid chromatography system with packed flow cell for improved fluorescence detection |
| US4285698A (en) * | 1980-04-28 | 1981-08-25 | Peanut Research & Testing Laboratories, Inc. | Analysis of aflatoxins in peanuts by high pressure liquid chromatograph |
| CN1556391A (en) * | 2003-12-30 | 2004-12-22 | 中国农业科学院油料作物研究所 | Peanut aspergillus flavus anti aflatoxin production preperty identification method |
| CN101246105A (en) * | 2008-01-16 | 2008-08-20 | 东北电力大学 | Method and device for measuring anti-dirty performance of material surface based on weight method |
| CN101285834A (en) * | 2008-06-02 | 2008-10-15 | 上海纤检仪器有限公司 | Method for accurately and rapidly checking aspergillus flavus toxin |
| CN101949854A (en) * | 2010-09-14 | 2011-01-19 | 云南健牛生物科技有限公司 | Method for rapidly detecting content of aflatoxin |
-
2012
- 2012-09-10 CN CN201210332642.2A patent/CN102818740B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4181853A (en) * | 1976-12-10 | 1980-01-01 | Varian Associates, Inc. | Liquid chromatography system with packed flow cell for improved fluorescence detection |
| US4285698A (en) * | 1980-04-28 | 1981-08-25 | Peanut Research & Testing Laboratories, Inc. | Analysis of aflatoxins in peanuts by high pressure liquid chromatograph |
| CN1556391A (en) * | 2003-12-30 | 2004-12-22 | 中国农业科学院油料作物研究所 | Peanut aspergillus flavus anti aflatoxin production preperty identification method |
| CN101246105A (en) * | 2008-01-16 | 2008-08-20 | 东北电力大学 | Method and device for measuring anti-dirty performance of material surface based on weight method |
| CN101285834A (en) * | 2008-06-02 | 2008-10-15 | 上海纤检仪器有限公司 | Method for accurately and rapidly checking aspergillus flavus toxin |
| CN101949854A (en) * | 2010-09-14 | 2011-01-19 | 云南健牛生物科技有限公司 | Method for rapidly detecting content of aflatoxin |
Non-Patent Citations (10)
| Title |
|---|
| 周桂元等.抗黄曲霉侵染花生品种的引进鉴定和利用.《花生学报》.2002,第31卷(第2期),14-17. |
| 国外引种花生对黄曲霉菌的抗性鉴定;黎穗临等;《花生科技》;20011231(第2期);13-16 * |
| 抗黄曲霉侵染花生品种的引进鉴定和利用;周桂元等;《花生学报》;20021231;第31卷(第2期);14-17 * |
| 李艳等.花生品质性状与抗黄曲霉侵染关系的研究.《花生学报》.2006,第35卷(第3期),1-5. |
| 梁炫强等.花生种子受黄曲霉菌侵染后若干生化成份的变化及其与抗性的关系.《中国油料作物学报》.2001,第23卷(第2期),26-30. |
| 梁炫强等.花生种皮蜡质和角质层与黄曲霉侵染和产毒的关系.《热带亚热带植物学报》.2003,第11卷(第1期),11-14. |
| 花生品质性状与抗黄曲霉侵染关系的研究;李艳等;《花生学报》;20061231;第35卷(第3期);1-5 * |
| 花生种子受黄曲霉菌侵染后若干生化成份的变化及其与抗性的关系;梁炫强等;《中国油料作物学报》;20010630;第23卷(第2期);26-30 * |
| 花生种皮蜡质和角质层与黄曲霉侵染和产毒的关系;梁炫强等;《热带亚热带植物学报》;20031231;第11卷(第1期);11-14 * |
| 黎穗临等.国外引种花生对黄曲霉菌的抗性鉴定.《花生科技》.2001,(第2期),13-16. |
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