CN102813773A - Compound traditional Chinese medicine for treating endometriosis and preparation method and application thereof - Google Patents
Compound traditional Chinese medicine for treating endometriosis and preparation method and application thereof Download PDFInfo
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- CN102813773A CN102813773A CN2012103106691A CN201210310669A CN102813773A CN 102813773 A CN102813773 A CN 102813773A CN 2012103106691 A CN2012103106691 A CN 2012103106691A CN 201210310669 A CN201210310669 A CN 201210310669A CN 102813773 A CN102813773 A CN 102813773A
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Abstract
The invention discloses compound traditional Chinese medicine for treating endometriosis. The compound traditional Chinese medicine comprises, by weight, 8-12 parts of Pollen Typhae, 8-12 parts of trogopterus dung, 1-3 parts of dragon's blood, 12-20 parts of astragali radix, 8-12 parts of dipsacus root, 8-12 parts of roasted myrrh, 8-12 parts of corydalis yanhusuo, and 8-12 parts of szechwan chinaberry fruit. The invention further discloses a preparation method and application of the compound traditional Chinese medicine. The compound traditional Chinese medicine brings the advantages of multi-targeted treating of traditional Chinese medicine into full play. Lab observation of development of ectopic endometrium in a drug inhibited rat endometriosis model shows that the compound traditional Chinese medicine can inhibit development of endometriosis in terms of inhibiting cell proliferation, angiogenesis and invasion in ectopic endometrium tissues, lowering inflammatory cell factor level in peritoneal fluid and the like.
Description
Technical field
The invention belongs to technical field of Chinese medicines, be specifically related to a kind of herbal mixture of treating endometriosis.
Background technology
Endometriosis is to have the common gynecological disease that active endometrial tissue appears at position beyond the intra-uterine membranous layer.Endometriosis is optimum morphology performance on pathology, but has the ability of similar malignant tumor plantation, invasion and attack and metastasis.Endometrial plantation of dystopy and invasion and attack depend on competent angiogenesis and blood supply.The change of peritoneal immunity environment in uterus plays an important role in the incidence and development of endometriosis.Between the peritoneal fluid inner cell factor and and cell between interaction be that endometrial plantation of dystopy and invasion and attack provide adapt circumstance.
Laparoscopic surgery and gonadotropin releasing hormone analogues (GnRHa) are the prefered methods of treatment endometriosis at present.Yet operative treatment is prone to cause adhesion and causes postoperative bellyache and infertile, and the Pathophysiology mechanism of causing a disease of the focus of not excision and not correction can cause recurrence; The GnRHa medicine can cause hypophysis-hypothalamic pituitary ovarium axis hypofunction, causes to be prone to recurrence after similar menopausal syndrome and the drug withdrawal in a short time.The Chinese medicine endometriosis is superior to Western medicine at aspects such as relieve clinical symptoms, regulating menstruation and assisting pregnancies, and toxic and side effects is little, can take for a long time.Therefore seek effect and reliable curative Chinese medicine, have crucial meaning for avoiding taking merely the untoward reaction that western medicine should disease occurs.
Summary of the invention
Technical problem to be solved by this invention provides the herbal mixture of the treatment endometriosis that a kind of efficient is high, toxic and side effects is little.
The technical problem that the present invention also will solve provides the method for preparing of above-mentioned herbal mixture.
The technical problem that the present invention will solve at last provides the application of above-mentioned herbal mixture in preparation treatment endometriosis medicine.
For solving the problems of the technologies described above, the technical scheme that the present invention adopts is following:
A kind of herbal mixture of treating endometriosis; It is characterized in that it comprises the component of following parts by weight: 8 ~ 12 parts of Pollen Tyjphae, 8 ~ 12 parts of Oletum Trogopteroris, 1 ~ 3 part of Sanguis Draxonis, 12 ~ 20 parts of Radix Astragali, 8 ~ 12 parts of Radix Dipsaci, 8 ~ 12 parts of Myrrha (processed)s, 8 ~ 12 parts of Rhizoma Corydalis, 8 ~ 12 parts of Fructus Toosendans.
The herbal mixture of above-mentioned treatment endometriosis preferably includes the component of following parts by weight: 10 parts of Pollen Tyjphae, 10 parts of Oletum Trogopteroris, 2 parts of Sanguis Draxonis, 15 parts of Radix Astragali, 10 parts of Radix Dipsaci, 10 parts of Myrrha (processed)s, 10 parts of Rhizoma Corydalis, 10 parts of Fructus Toosendans.
The method for preparing of the herbal mixture of above-mentioned treatment endometriosis; Pollen Tyjphae, Oletum Trogopterori, Radix Astragali, Radix Dipsaci, Myrrha (processed), Rhizoma Corydalis, the Fructus Toosendan of formula ratio are mixed, soak, decoct twice; Each big fire is fried in shallow oil out the little fire in back and was fried in shallow oil 1 ~ 2 hour; Leach medicinal liquid, merge the medicinal liquid that leaches for twice and mix and concentrate, the medicinal liquid that the Powdered Sanguis Draxonis of formula ratio directly adds after concentrated promptly gets.
Wherein, the time of soaking is 30 minutes.
Wherein, the weight that adds water is Pollen Tyjphae, Oletum Trogopterori, Radix Astragali, Radix Dipsaci, Myrrha (processed), Rhizoma Corydalis and Fructus Toosendan gross weight 15 ~ 20 times.
The application of the herbal mixture of above-mentioned treatment endometriosis in preparation treatment endometriosis medicine.
Beneficial effect: herbal mixture of the present invention has been brought into play the cure the disease advantage of many target spots of Chinese medicine; The laboratory observation that suppresses dystopy endometrium development in the rat endometrium dystopy disease model through medicine; Find that Chinese medicine compound of the present invention can cell proliferation, angiogenesis and invasion and attack in suppressing the dystopy endometrial tissue, reduce the development that aspect such as inflammatory cytokine level in the peritoneal fluid suppresses endometriosis.
Description of drawings
The volume of endometriosis cyst before and after the treatment of Fig. 1: embodiment 1 herbal mixture.
Fig. 2: embodiment 1 herbal mixture suppresses the immunohistochemical analysis photo of endometriotic tissues propagation.
Fig. 3: embodiment 1 herbal mixture suppresses dystopy endometrium medium vessels and generates.
Fig. 4: embodiment 1 herbal mixture suppresses the invasion and attack of dystopy endometrium.
The level of TNF-α and IL-1 β in Fig. 5: the embodiment 1 herbal mixture reduction endometriosis peritoneal fluid.
The specific embodiment
According to following embodiment, can understand the present invention better.Yet, those skilled in the art will readily understand that the described content of embodiment only is used to explain the present invention, and the present invention that should also can not limit in claims to be described in detail.
Embodiment 1:
The herbal mixture of above-mentioned treatment endometriosis comprises the component of following parts by weight: 10 parts of Pollen Tyjphae, 10 parts of Oletum Trogopteroris, 2 parts of Sanguis Draxonis, 15 parts of Radix Astragali, 10 parts of Radix Dipsaci, 10 parts of Myrrha (processed)s, 10 parts of Rhizoma Corydalis, 10 parts of Fructus Toosendans.
Method for preparing; Pollen Tyjphae, Oletum Trogopterori, Radix Astragali, Radix Dipsaci, Myrrha (processed), Rhizoma Corydalis, the Fructus Toosendan of formula ratio are mixed, and the water logging that adds 16 times of weight was steeped 30 minutes, decocted twice; Each big fire is fried in shallow oil out the little fire in back and was fried in shallow oil 1.5 hours; Leach medicinal liquid, merge the medicinal liquid that leaches for twice and mix and concentrate, the medicinal liquid that the Powdered Sanguis Draxonis of formula ratio directly adds after concentrated promptly gets.
Embodiment 2:
The herbal mixture of above-mentioned treatment endometriosis comprises the component of following parts by weight: 8 parts of Pollen Tyjphae, 8 parts of Oletum Trogopteroris, 1 part of Sanguis Draxonis, 12 parts of Radix Astragali, 8 parts of Radix Dipsaci, 8 parts of Myrrha (processed)s, 8 parts of Rhizoma Corydalis, 8 parts of Fructus Toosendans.
Method for preparing; Pollen Tyjphae, Oletum Trogopterori, Radix Astragali, Radix Dipsaci, Myrrha (processed), Rhizoma Corydalis, the Fructus Toosendan of formula ratio are mixed, and the water logging that adds 15 times of weight was steeped 30 minutes, decocted twice; Each big fire is fried in shallow oil out the little fire in back and was fried in shallow oil 1 hour; Leach medicinal liquid, merge the medicinal liquid that leaches for twice and mix and concentrate, the medicinal liquid that the Powdered Sanguis Draxonis of formula ratio directly adds after concentrated promptly gets.
Embodiment 3:
The herbal mixture of above-mentioned treatment endometriosis comprises the component of following parts by weight: 12 parts of Pollen Tyjphae, 12 parts of Oletum Trogopteroris, 3 parts of Sanguis Draxonis, 20 parts of Radix Astragali, 12 parts of Radix Dipsaci, 12 parts of Myrrha (processed)s, 12 parts of Rhizoma Corydalis, 12 parts of Fructus Toosendans.
Method for preparing; Pollen Tyjphae, Oletum Trogopterori, Radix Astragali, Radix Dipsaci, Myrrha (processed), Rhizoma Corydalis, the Fructus Toosendan of formula ratio are mixed, and the water logging that adds 20 times of weight was steeped 30 minutes, decocted twice; Each big fire is fried in shallow oil out the little fire in back and was fried in shallow oil 2 hours; Leach medicinal liquid, merge the medicinal liquid that leaches for twice and mix and concentrate, the medicinal liquid that the Powdered Sanguis Draxonis of formula ratio directly adds after concentrated promptly gets.
Embodiment 4: herbal mixture of the present invention suppresses the development of endometriosis in rat endometrium dystopy disease model.
1. the foundation of rat endometrium dystopy disease model:
10% chloral hydrate solution carries out intraperitoneal injection of anesthesia (0.7mL/200g), and rat is lain on the back to be fixed on the operating-table, cuts off ventral seta, routine disinfection.Take off median abdominal incision, upwards cut 3cm from superior margin of pubic symphysis 0.5cm place.Uterus, free left side, near-end is from left cornua uteri 1cm place's ligation, and far-end goes out ligation from ovary 1cm.The uterine cancer cell that downcuts is put into the culture dish that fills physiological saline solution, and stringer is cut open, cuts the endometrial tissue of 5cm * 5cm.Between abdominal muscle and subcutaneous fascia layer, beat the tunnel on the incision of abdominal wall right side, the endometrial tissue piece is entirely inserted the right side tunnel bottom, make lining endothelium be close to abdominal muscle, the conventional abdomen that closes.Sham operated rats is not implanted endometrial tissue, the same model group of other steps.Cut open the belly for the second time after 28 days, the growing state of inspection endometriosis kitchen range, and with the formed cyst size of kind of calliper.
2. divide into groups and dosage regimen:
The rat that modeling is successful is divided into 3 groups (model control group, embodiment 1 herbal mixture low dose group, embodiment 1 herbal mixture high dose group) at random, and each group is pressed following scheme administration:
(1) embodiment 1 herbal mixture low dose group: every day, every rat gave 8.57g/kg embodiment 1 herbal mixture;
(2) embodiment 1 herbal mixture high dose group: every day, every rat gave 17.14g/kg embodiment 1 herbal mixture;
(3) model control group: every day, every rat gave the equal-volume distilled water.
(4) sham operated rats: every day, every rat gave the equal-volume distilled water.
Each is organized rat and is the administration by gavage administration, continuous 4 weeks.After treating for 4 weeks, collect rat abdominal cavity liquid, the ELISA method detects the concentration of VEGF, TNF-α and IL-1 β; With kind of calliper rat endometrium dystopy disease cyst volume (0.52 * major diameter * short axis minor axis
2); Endometriosis cyst (sham operated rats is got the normal uterus inner membrance) is divided into two parts; Portion is fixed with 10% neutral formalin solution; The SABC method detects cell proliferation (PCNA antibody staining) and vessel density (CD31 antibody staining); Another part is used to extract RNA, and the real-time fluorescence quantitative PCR method detects the expression (β-Actin is confidential reference items) of MMP-9 and TIMP-1.
Primer: MMP-9forward:5 '-CCG CGT GTA CGG GCT CGA AG-3 '
MMP-9reverse:5’-GCC?TTT?GCC?CAG?CGA?CCA?CA-3’
TIMP-1forward:5’-TGC?CCC?AAC?CCA?CCC?ACA?GA-3’
TIMP-1reverse:5’-GCT?GGG?GTG?TAG?GCG?AAC?CG-3’
β-Actin?forward:5’-CAC?CCG?CGA?GTA?CAA?CCT?TC-3’
β-Actin?reverse:5’-CCC?ATA?CCC?ACC?ATC?ACA?CC-3’
3. experimental result
(1) embodiment 1 herbal mixture significantly reduces endometriosis cyst volume.
Each organizes rat endometriosis cyst volume before treatment does not have significant difference.Through the treatment of 4 weeks, embodiment 1 herbal mixture significantly reduces endometriosis cyst volume, sees Fig. 1.
(2) embodiment 1 herbal mixture significantly suppresses endometriotic tissues propagation.
PCNA+ cell proportion is apparently higher than sham operated rats in the model of endometriosis group.PCNA+ cell proportion is starkly lower than matched group in the embodiment 1 herbal mixture treatment group.The result sees Fig. 2.
(3) embodiment 1 herbal mixture significantly suppresses the endometriosis angiogenesis.
(4) embodiment 1 herbal mixture suppresses the endometrial invasion and attack of dystopy.
(5) level of inflammatory cytokine TNF-α and IL-1 β in the embodiment 1 herbal mixture inhibition endometriosis peritoneal fluid.
The level of TNF-α and IL-1 β is apparently higher than sham operated rats in the model of endometriosis group peritoneal fluid.Embodiment 1 herbal mixture significantly reduces the level of these two kinds of inflammatory cytokines, and level and the sham operated rats of TNF-α and IL-1 β is approaching in the high dose embodiment 1 herbal mixture treatment group peritoneal fluid, sees Fig. 5.
Claims (6)
1. herbal mixture of treating endometriosis; It is characterized in that it comprises the component of following parts by weight: 8 ~ 12 parts of Pollen Tyjphae, 8 ~ 12 parts of Oletum Trogopteroris, 1 ~ 3 part of Sanguis Draxonis, 12 ~ 20 parts of Radix Astragali, 8 ~ 12 parts of Radix Dipsaci, 8 ~ 12 parts of Myrrha (processed)s, 8 ~ 12 parts of Rhizoma Corydalis, 8 ~ 12 parts of Fructus Toosendans.
2. the herbal mixture of treatment endometriosis according to claim 1; It is characterized in that it comprises the component of following parts by weight: 10 parts of Pollen Tyjphae, 10 parts of Oletum Trogopteroris, 2 parts of Sanguis Draxonis, 15 parts of Radix Astragali, 10 parts of Radix Dipsaci, 10 parts of Myrrha (processed)s, 10 parts of Rhizoma Corydalis, 10 parts of Fructus Toosendans.
3. the method for preparing of the herbal mixture of the described treatment endometriosis of claim 1 is characterized in that, with Pollen Tyjphae, Oletum Trogopterori, Radix Astragali, Radix Dipsaci, Myrrha (processed), Rhizoma Corydalis, the Fructus Toosendan mixing of formula ratio; Soak; Decoct twice, each big fire is fried in shallow oil out the little fire in back and was fried in shallow oil 1 ~ 2 hour, leaches medicinal liquid; Merge the medicinal liquid that leaches for twice and mix and concentrate, the medicinal liquid that the Powdered Sanguis Draxonis of formula ratio directly adds after concentrated promptly gets.
4. the method for preparing of the herbal mixture of treatment endometriosis according to claim 3 is characterized in that, the time of soaking is 30 minutes.
5. the method for preparing of the herbal mixture of treatment endometriosis according to claim 3 is characterized in that, the weight that adds water is Pollen Tyjphae, Oletum Trogopterori, Radix Astragali, Radix Dipsaci, Myrrha (processed), Rhizoma Corydalis and Fructus Toosendan gross weight 15 ~ 20 times.
6. the application of the herbal mixture of the described treatment endometriosis of claim 1 in preparation treatment endometriosis medicine.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103110921A (en) * | 2013-02-20 | 2013-05-22 | 李海霞 | Externally-applied TCM (traditional Chinese medicine) preparation for treating endometriosis |
| CN104906296A (en) * | 2015-06-11 | 2015-09-16 | 广西中医药大学 | Traditional Chinese medicine preparation for treating endometriosis and preparation method of traditional Chinese medicine preparation |
| CN105944033A (en) * | 2016-06-27 | 2016-09-21 | 江苏省中医药研究院 | Traditional Chinese medicine composition containing radix curcumae |
| CN109078143A (en) * | 2018-10-25 | 2018-12-25 | 上海中医药大学附属岳阳中西医结合医院 | A kind of Chinese medicine composition that treating endometriosis and its application |
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2012
- 2012-09-28 CN CN201210310669.1A patent/CN102813773B/en active Active
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| 卢翠云 等: "《子宫内膜异位症中医治疗概况》", 《江西中医药》, vol. 32, no. 3, 30 June 2001 (2001-06-30) * |
| 王艳丽 等: "《活血化瘀法治疗子宫内膜异位症的临床观察》", 《中国实用医药杂志》, vol. 2, no. 14, 31 May 2007 (2007-05-31) * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103110921A (en) * | 2013-02-20 | 2013-05-22 | 李海霞 | Externally-applied TCM (traditional Chinese medicine) preparation for treating endometriosis |
| CN103110921B (en) * | 2013-02-20 | 2014-11-19 | 沈红 | Externally-applied TCM (traditional Chinese medicine) preparation for treating endometriosis |
| CN104906296A (en) * | 2015-06-11 | 2015-09-16 | 广西中医药大学 | Traditional Chinese medicine preparation for treating endometriosis and preparation method of traditional Chinese medicine preparation |
| CN105944033A (en) * | 2016-06-27 | 2016-09-21 | 江苏省中医药研究院 | Traditional Chinese medicine composition containing radix curcumae |
| CN105944033B (en) * | 2016-06-27 | 2020-05-01 | 江苏省中医药研究院 | Traditional Chinese medicine composition containing radix curcumae |
| CN109078143A (en) * | 2018-10-25 | 2018-12-25 | 上海中医药大学附属岳阳中西医结合医院 | A kind of Chinese medicine composition that treating endometriosis and its application |
| CN109078143B (en) * | 2018-10-25 | 2020-12-15 | 上海中医药大学附属岳阳中西医结合医院 | Traditional Chinese medicine composition for treating endometriosis and application thereof |
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