CN102788790A - Novel colorimetric test pen - Google Patents

Novel colorimetric test pen Download PDF

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Publication number
CN102788790A
CN102788790A CN2012102441550A CN201210244155A CN102788790A CN 102788790 A CN102788790 A CN 102788790A CN 2012102441550 A CN2012102441550 A CN 2012102441550A CN 201210244155 A CN201210244155 A CN 201210244155A CN 102788790 A CN102788790 A CN 102788790A
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open
ground
colorimetric
flexible pipe
storage chamber
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CN2012102441550A
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Chinese (zh)
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申贵隽
张寒格
王颖
谢弘宇
申远
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Dalian University
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Dalian University
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  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention discloses a novel colorimetric test pen, which is obtained by reconstructing and innovating based on common syringes in markets, is a liquid taking device adjusted by double pressures, and is designed for the first time according to the design principles of pH test paper, proceeding from the color reaction of objects under test. The test pen disclosed herein is a simple and convenient test pen for detection which combines sampling, releasing developer and color comparison as a whole. The test pen disclosed herein meets the requirements of people for instant detection, and is a popular, simple and portable testing arrangement.

Description

A kind of novel colorimetric examination pen
Technical field
The present invention relates to the physico-chemical analysis instrument field, be specifically related to a kind of novel colorimetric examination pen.
Background technology
Along with the development of society, the raising of people's living standard, people require mechanism of COMM HLTH to provide to comprise laboratory inspection except will having main equipment, more advanced Interventions Requested; Also should walk out the laboratory; Towards community, come into family, towards the rural area; This also just requires on inspection machine and experimental technique, to improve to some extent, and the scene that makes it more can carry out is detected.For example how to make do not accept professional training personnel all (like family's self check) or part (like color contrast) in time fast detecting go out in the ordinary powdered milk content of melamine and whether exceed standard etc.In some cases, traditional centralab's pattern has satisfied not the demand that this fast pace of people is lived.
Summary of the invention
For addressing the above problem, the present invention transforms innovation on the basis of ordinary syringe on the market, and designing collection sampling, developer release and colorimetric estimation is the simple portable dedicated inspection test pen of one.This novel colorimetric examination pen, this invention based on measure objectionable impuritiess such as animals and plants food, environment and vegetable and fruit residual with the chemical analysis technology that adds quantity, can in several minutes, obtain the concentration of determinand, satisfied people's demand to a certain extent.
A kind of novel colorimetric of the present invention is tried pen, comprises a body of syringe-like,
On a body top open S is arranged 1(31), open S 1(31) through having flow regulation folder S 1(12) flexible pipe S 1(11) be connected with syringe needle 1, a body inside be provided with the band rubber piston 33 lift the tube 35, a body side wall markings has scale 34, a body outer wall is provided with normal concentration colorimetric card 36;
The inner space that body top and rubber piston 33 surround is that chrominance response chamber 32 is provided with open S near a sidewall on body top 2(21);
Be provided with the nested district of ground Q at the said tube 35 afterbody outer walls that lift 1(37), through the nested district of ground Q 1(37) side with cup-shaped is provided with open S 3(42) developer storage chamber 41 tablings;
Outer wall is provided with the nested district of ground Q at the bottom of the chamber in developer storage chamber 41 254, and through the nested district of ground Q 254 with the side be provided with open S 4 Detachable storage chamber 51 tablings of 53 cup-shaped;
The cup end in said detachable storage chamber 51, is through the nested district of ground Q 352 with ground cover 38 tablings;
Described open S 221, open S 342 through flexible pipe S 222 connect;
Perhaps, open S 221, open S 453 through flexible pipe S 222 connect;
Described flexible pipe S 2Have flow regulation folder S on 22 223.
Above-mentioned colorimetric examination pen is the collection sampling, discharge developer, colorimetric is an one, is used for the mensuration of multiple testing concentration scope, installs simple and easyly, and disposable use carries, operates more convenient.This device is formed by the ordinary syringe transformation, and be about to the syringe slide and change system into inner chamber with storage developer function, in the syringe chromogenic reaction district, have the normal concentration colorimetric card than coloured pencil outer wall.
The making principle of normal concentration colorimetric card is; According to the color reaction that determinand had, dispose the concentration of standard solution gradient of a series of determinands, and respectively with the developer generation color reaction of itself and same amount; According to the products therefrom color; Make the normal concentration colorimetric card, place the outer wall surface of colorimetric examination pen container wall, be used for reading fast of testing concentration.
Described flow regulation folder may command dynamics can be according to the dynamics of flow regulation folder, the turnover speed and the inlet of control solution.When chrominance response is comparatively simple; Like common A+B type reaction; Require developer and liquid to be measured to enter into simultaneously when reacting in the examination pen; Available common spring clamp replaces above-mentioned flow regulation folder, directly passes through the change of the internal diameter of two flexible pipes, makes liquid to be measured and developer enter into simultaneously in certain proportion in the examination pen.
The examination pen lifts an interior rubber piston and developer storage chamber (its inside can add a certain amount of developer) separates; Meanwhile; Can also make a series of different models, with match, the connect detachable storage chamber of use, developer storage chamber, nested as one sees fit according to the consumption and the kind of the required developer of determinand color reaction.
Choosing of a colorimetric examination material; For the conventional injector syringe can be that plastics also can be that glass is processed, but because the present invention is disposable use, and plastics machinery intensity, stretching and shock proof ability are higher; In light weight; Be convenient to processing, lower with the processing cost of its making, therefore be selected in polypropylene and make this colorimetric examination pen.
Ratio according to testing sample and developer in the experiment; Can change the internal diameter of the flexible pipe that is connected to testing sample and developer respectively; When last lift tin, open the flow regulation folder on the flexible pipe simultaneously, then developer and testing sample enter in the examination in certain proportion simultaneously.Flexible pipe with same inner diameter is connected respectively to liquid to be measured and developer, and on flexible pipe, presss from both sides with flow regulation, is used for the control of flow.
The present invention has following characteristics:
1) can collect sampling, discharge developer, colorimetric and concentration range be read as one, combine the function of laboratory part instrument.
2) detection speed is fast, can in several minutes, read the concentration of determinand, and has certain sensitivity and selectivity.
3) simple in structure, easy to carry, be fit to very much field quick detection.
4) simple to operate, the user does not need special training just can grasp.
5) the device low price does not need repair and maintenance, disposable use.
6) the test solution expense is few, and wastage is few, and finding speed is high.
7) applied range can be widely used in fields such as chemistry, metallurgy, health care, agricultural, geology, environmental protection.
Description of drawings
Fig. 1: a colorimetric examination structural representation before the sampling; Wherein, 1. syringe needle; 11. flexible pipe S 112. flow regulation folder S 121. open S 222. flexible pipe S 223. flow regulation folder S 231. open S 132. chrominance response chamber; 33. rubber piston; 34. scale; 35. lift tube; 36. normal concentration colorimetric card; 37. the nested district of ground Q 138. ground lid; 41. developer storage chamber; 42. open S 351. detachably store the chamber; 52. the nested district of ground Q 353. vent cover; 54. the nested district of ground Q 2
Fig. 2: the interpolation process of reaction reagent;
Fig. 3: the sketch of normal concentration colorimetric card.
Embodiment
Following non-limiting example can make those of ordinary skill in the art more fully understand the present invention, but does not limit the present invention in any way.
Embodiment 1
With sodium nitrite Determination on content in the food is example, sketches the concrete method of application of this contrive equipment.
(1) making of normal concentration colorimetric card
A) make principle.After sodium nitrite and the sulfanilic acid acid amides diazotising; Generate the aubergine compound with hydrochloride naphthodiamide generation coupling reaction again; Variable concentrations gradient sodium nitrite standard solution is participated in color difference that reaction appears (the concrete assay method document that sees reference: the mensuration of content of nitrite [J] in the food; The Hebei chemical industry, 2010,33 (3); 62-63).
B) method for making.Accurately pipette the NaNO of 5 μ g/mL 2Operation liquid 0,0.5,1.0,2.0,3.0,4.0,5.0mL place the 25mL color comparison tube respectively, in standard pipe, add the 4.5mL ammonium chloride buffer respectively, add the 5.0mL developer immediately after adding 2.5mL60% acetic acid, add water to scale, mixing.Present color according to reaction and describe out with drawing software on computers, print with color inkjet printer again, promptly can be made into normal concentration colorimetric card as shown in Figure 3, and it is invested the barrel of this colorimetric examination pen.Pairing color on each concentration of sodium nitrite and the normal concentration colorimetric card.
By the content of sodium nitrite in the following formula calculation sample, result of calculation is as shown in table 1:
X = c · V / m = c · ug / mL × 100 mL × 10 - 3 mg / ug 5.000 g × 10 - 3 kg / g = 20 cmg / kg
(the pairing concentration of c---normal concentration colorimetric card color)
(content of nitrite in every kilogram of sample of X---)
Table 1 concentration of standard solution and the contrast of colorimetric card color
Figure BDA00001887826800032
The pairing concentration of standard solution of colorimetric card different colours is: A:0.0 μ g/mL, B:0.5 μ g/mL, C:1.0 μ g/mL, D:1.5 μ g/mL, E:2.0 μ g/mL, F:2.5 μ g/mL, G:3.0 μ g/mL;
The corresponding NaNO2 content of colorimetric card various criterion solution concentration is: A:0.00mg/kg, B:10.00mg/kg, C:20.00mg/kg, D:30.00mg/kg, E:40.00mg/kg, F:50.00mg/kg, G:60.00mg/kg.
(2) detect content of sodium nitrite in the meat products with this device
A) sample source.No. 1 sample: golden gong board seafood intestines; No. 2 samples: two remittance boards are baked intestines; 3, No. 4 samples: businessman is from the roasting intestines of processing.According to list of references (Hebei chemical industry, 2010,33 (3); The sample treatment of 62-63) being reported obtains corresponding testing sample test solution.
B) original state of colorimetric examination pen:
Rubber piston 33 is in open S 221 sides near the tail end place of a body are full of air in the chrominance response chamber 32.
C) process of absorption sample:
Open flow regulation folder S 112, close flow regulation folder S 223, the syringe needle 1 of colorimetric examination pen is inserted in the testing sample test solution of having handled well, what slowly lift colorimetric examination pen lifts tube 35, makes rubber piston 33 move the graduation range of 0.4mL.At this moment, under the effect of external pressure, 0.4mL testing sample test solution is pumped in the chrominance response chamber 32 lentamente.Close flow regulation folder S 112.
D) method of ammonium chloride buffer interpolation:
Get developer storage chamber 41, as shown in Figure 2, through open S 342 to wherein adding ammonium chloride buffer, and in open S 342 places connect and have flow regulation folder S 223 flexible pipe S 222, flexible pipe S 222 the other end and open S 221 connect.Open flow regulation folder S 2After 23, the colorimetric that slowly tilts an examination pen body makes the 0.25mL ammonium chloride buffer flow in the chrominance response chamber 32, closes flow regulation folder S 2After 23, rock colorimetric examination pen, make the liquid mixing in the chrominance response chamber 32.
E) method of 60% acetic acid interpolation:
The interpolation process of reaction reagent is as shown in Figure 2, gets a detachable storage chamber 51, to wherein adding 60% acetic acid, and in open S 453 places connect the flexible pipe that has the flow regulation folder, the other end of flexible pipe and open S 221 connect.Open flow regulation folder S 2After 23, inclination colorimetric examination pen makes 60% acetic acid of 0.15mL flow in the chrominance response chamber 32, closes flow regulation folder S 2After 23, rock colorimetric examination pen, make the liquid mixing in the chrominance response chamber 32.
F) method of developer interpolation:
Take off detachable storage chamber 51, the bottom, chamber in detachable storage chamber 41 is through the nested district of ground Q 254 one of series connection have the detachable storage chamber with spline structure with it, and repeat step e), in a colorimetric examination chrominance response chamber 32, add the developer of 0.2mL, rock colorimetric examination, make the interior liquid mixing in chrominance response chamber 32.
G) judgement of testing result:
After treating that color reaction is stablized in the chrominance response chamber 32, the similarities and differences of observing product color and normal concentration colorimetric card 36, color and corresponding NaNO thereof like selection is nearest 2Concentration obtains the relevant detection result according to table 2.
Sodium nitrite assay result relatively in table 2 meat products
Figure BDA00001887826800051
Annotate: No. 1 sample: golden gong board seafood intestines; No. 2 samples: two remittance boards are baked intestines; 3, No. 4 samples: businessman is from the roasting intestines of processing.
Design concept according to the pH test paper; Color reaction by determinand sets out, and need the normal concentration colorimetric card on the colorimetric examination pen be changed into by the respective standard concentration gradient following of the condition that changes developer and can accomplish the assay determination to this sample in the resulting colorimetric card of determinand color reaction.Design two liquid taking devices of pressing adjustment first.The test solution expense is few, and wastage is few, and finding speed is high, is with a wide range of applications.

Claims (1)

1. a novel colorimetric is tried pen; A body that comprises syringe-like; It is characterized in that: there is open S 1 (31) on a body top, and open S 1 (31) is connected with syringe needle (1) through the flexible pipe S1 (11) that has flow regulation folder S1 (12), and a body inside is provided with lift tin (35) of band rubber piston (33); Body side wall markings has scale (34), and a body outer wall is provided with normal concentration colorimetric card (36);
The inner space that body top and rubber piston (33) surround is chrominance response chamber (32) near a sidewall on body top, is provided with open S 2 (21);
Be provided with the nested district of ground Q1 (37) at said tube (35) the afterbody outer wall that lifts, be provided with developer storage chamber (41) tabling of open S 3 (42) through the side of ground nested district Q1 (37) and cup-shaped;
Outer wall is provided with the nested district of ground Q2 (54) at the bottom of the chamber in developer storage chamber (41), and is provided with detachable storage chamber (51) tabling of the cup-shaped of open S 4 (53) through ground nested district Q2 (54) and side;
The cup end in said detachable storage chamber (51), is through the nested district of ground Q3 (52) and ground lid (38) tabling;
Described open S 2 (21), open S 3 (42) connect through flexible pipe S2 (22);
Perhaps, open S 2 (21), open S 4 (53) connect through flexible pipe S2 (22);
Have flow regulation folder S2 (23) on the described flexible pipe S2 (22).
CN2012102441550A 2012-03-01 2012-07-13 Novel colorimetric test pen Pending CN102788790A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104267027A (en) * 2014-10-17 2015-01-07 北京理工大学 Method for detecting target object by utilizing color-developable imprinting colloid film
CN110940661A (en) * 2019-11-12 2020-03-31 大连诚泽检测有限公司 Suction-type quantitative analysis device

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1501069A (en) * 2002-11-15 2004-06-02 中国农业大学 Preparing and using method for test paper detecting ozone
CN201037828Y (en) * 2007-02-07 2008-03-19 中华人民共和国海南出入境检验检疫局 Portable fast detecting device for formaldehyde in textile
US20090155923A1 (en) * 2005-11-08 2009-06-18 Gerhard Bonecker Photometric Measuring Method for a Sample Liquid, A Photometric Measuring Device, and a Mixing Container for a Photometric Measuring Device
CN101294912B (en) * 2008-06-04 2010-07-14 山西大学 Tea polyphenol detecting test paper, standard color comparison card and usage thereof
CN201773068U (en) * 2010-03-12 2011-03-23 韩少春 Multivariate test paper capable of testing pesticide residues of fruits and vegetables

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1501069A (en) * 2002-11-15 2004-06-02 中国农业大学 Preparing and using method for test paper detecting ozone
US20090155923A1 (en) * 2005-11-08 2009-06-18 Gerhard Bonecker Photometric Measuring Method for a Sample Liquid, A Photometric Measuring Device, and a Mixing Container for a Photometric Measuring Device
CN201037828Y (en) * 2007-02-07 2008-03-19 中华人民共和国海南出入境检验检疫局 Portable fast detecting device for formaldehyde in textile
CN101294912B (en) * 2008-06-04 2010-07-14 山西大学 Tea polyphenol detecting test paper, standard color comparison card and usage thereof
CN201773068U (en) * 2010-03-12 2011-03-23 韩少春 Multivariate test paper capable of testing pesticide residues of fruits and vegetables

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104267027A (en) * 2014-10-17 2015-01-07 北京理工大学 Method for detecting target object by utilizing color-developable imprinting colloid film
CN110940661A (en) * 2019-11-12 2020-03-31 大连诚泽检测有限公司 Suction-type quantitative analysis device

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Application publication date: 20121121