CN102787068A - Sample applicator - Google Patents
Sample applicator Download PDFInfo
- Publication number
- CN102787068A CN102787068A CN2012102550539A CN201210255053A CN102787068A CN 102787068 A CN102787068 A CN 102787068A CN 2012102550539 A CN2012102550539 A CN 2012102550539A CN 201210255053 A CN201210255053 A CN 201210255053A CN 102787068 A CN102787068 A CN 102787068A
- Authority
- CN
- China
- Prior art keywords
- point sample
- reagent
- chamber
- sequencing reaction
- reaction small
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/16—Microfluidic devices; Capillary tubes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
Abstract
The invention relates to the precise engineering field, and provides a sample applicator. The sample applicator comprises a control assembly, a microfluid assembly and a small sequencing reaction chamber, wherein the control assembly controls the reagent transmission of the microfluid assembly; the microfluid assembly is used for transmitting the reagent to the small sequencing reaction chamber; the small sequencing reaction chamber comprises at least one sample application cavity; and the interior surface of the small sequencing reaction chamber is physically or chemically treated. The sample applicator provided in the invention can realize the full-automation and high-quality sample application.
Description
Technical field
The present invention relates to the precision engineering field, more particularly, relate to a kind of point sample instrument.
Background technology
Point sample is that testing sample is fixed in FX, and the testing sample that will be fixed in certain FX then is fixed on the sequencing device.Sample carries out biochemical reaction in sequencing device, order-checking is adopted map device and carried out IMAQ then.The quality of point sample directly has influence on the quality of order-checking, such as: sample is rotten, causes follow-up sequencing result inaccurate; The density of point sample is too low, causes sequencing throughput to reduce greatly; The density of point sample is too high, and the image that causes order-checking to adopt the map device collection can't be distinguished, and also is that point sample is a vital step in the order-checking process.In early days, adopt manual mode that testing sample is fixed in FX, realize the point sample of FX, early stage technical scheme has been wasted the great amount of manpower resource, and the precision of point sample is not high simultaneously.Though along with the development of mechanical automation development of technology and nucleic acid sequencing, having produced a series of is the semi-automatic or full automatic point sample instruments that are applicable to different sequencing devices, still realize to the point sample of sequencing reaction small chamber at present through manual.
In the prior art, a kind of point sample instrument comprises parts compositions such as three-dimensional motion mechanism, point sample mechanism, supporting mechanism and control unit.Control unit is a computingmachine, the work of computer control three-dimensional motion mechanism and point sample mechanism.Wherein, Three-dimensional motion mechanism principle of work and structure are formed as follows: Z is fixed on X on moving parts to moving parts; X is arranged on the top end rail to moving parts, bottom end rail be fixed on the slide block of Y on moving parts and link to each other, X, Y, three direction moving partss of Z are by servo motor driven; This three-dimensional motion mechanism is connected with supporting mechanism; Point sample mechanism is made up of point needle, dried pin device, sample box, wafer-supporting platform; This point sample mechanism is connected with three-dimensional motion mechanism; Motion under the drive of three-dimensional motion mechanism, point needle reaches the replacing that dried pin device is realized point needle in the drive of three-dimensional motion mechanism, arrives sample box then and extracts reagent corresponding; Reagent is being sprayed onto in the wafer-supporting platform as porous plate through point needle, thereby is realizing point sample.In this technical scheme, because during point sample, sample reveals to be put in air, causes reagent contamination easily, or reagent is oxidized etc. and make sample rotten, thereby makes that follow-up sequencing result is inaccurate; Simultaneously, this point sample is through point needle reagent to be ejected in each hole, causes the density unevenness of point sample even.
Can know that to sum up the level of automation of prior art mid point model machine is low, and the quality of point sample is low.Need a kind of new point sample instrument, can realize full-automatic high-quality point sample.
Summary of the invention
The object of the present invention is to provide a kind of point sample instrument, it is low to be intended to solve prior art point sample level of automation, the problem such as of low quality of point sample.
In order to realize goal of the invention, a kind of point sample instrument comprises: control unit, microfluid component and sequencing reaction small chamber.Wherein, said control unit control microfluid component transmission reagent; Said microfluid component is used for reagent is transferred to sequencing reaction small chamber; Said sequencing reaction small chamber comprises at least one point sample chamber.The internal surface of said sequencing reaction small chamber is through physics or chemical treatment.Can be used for combining magnetic bead or DNA or RNA fragment through physics or chemically treated sequencing reaction small chamber internal surface.
Wherein, said sequencing reaction small chamber is provided with gauge point; Said gauge point is used to check order and adopts the automatic location of map device to sequencing reaction small chamber.
Wherein, said point sample instrument also comprises the temperature control assembly, and said temperature control assembly is connected with sequencing reaction small chamber, is used for sequencing reaction small chamber is heated or freezes; Said control unit also is used to control the work of temperature control assembly.
Wherein, said each point sample chamber includes reagent inlet, reagent outlet and point sample zone; Said reagent inlet is used to receive the reagent of microfluid component transmission; Said reagent outlet is used for the reagent in the output sequencer reaction small chamber; Said point sample zone is handled through hydroxylation or amination is handled, and is used to combine magnetic bead or DNA or RNA fragment.
Wherein, said sequencing reaction small chamber comprises the parallel diaphragm seal that carries print, deckglass and be provided with hole logical in a plurality of; Said diaphragm seal forms a plurality of point samples chamber jointly carrying between print and the deckglass.
Wherein, said year print is divided into a plurality of point samples zone by diaphragm seal.
Wherein, said gauge point is positioned at and carries on print or the deckglass.
Preferably, said reagent inlet lays respectively at the regional opposite end of each point sample with the reagent outlet.
In above-mentioned arbitrary technical scheme, said microfluid component comprises pump and containing liquid chamber; Said pump is connected with containing liquid chamber respectively, is used for the reagent of containing liquid chamber is transferred to the point sample chamber in the sequencing reaction small chamber; Said containing liquid chamber is stored at least a reagent.
Wherein, said microfluid component also comprises skimmer; Said skimmer is connected with the point sample chamber with pump respectively, is used to receive the reagent of pump transmission, and the reagent that receives is divided equally and imported to each point sample chamber.
Wherein, said microfluid component also comprises mechanical manipulator; Said mechanical manipulator is connected with pump, and the reagent that is used for extracting containing liquid chamber is in pump.
By on can know that the present invention is drawn into reagent in the sequencing reaction small chamber through control unit control microfluid component, has realized the full-automatic point sample of sequencing reaction small chamber.Simultaneously, arrive sequencing reaction small chamber, all are airtight environment, thereby reduced the pollution of air that in addition, reagent is filled whole point sample chamber, has guaranteed the homogeneity of point sample, thereby has realized high-quality point sample reagent from extracting reagent.
Description of drawings
Fig. 1 is the structural representation of point sample instrument in the first embodiment of the invention.
Fig. 2 is the part-structure synoptic diagram of point sample instrument in the first embodiment of the invention.
Fig. 3 is the structural representation of point sample instrument in the second embodiment of the invention.
Fig. 4 is the structural representation in point sample chamber in the third embodiment of the invention.
Fig. 5 is the structural representation of sequencing reaction small chamber in the fourth embodiment of the invention.
Fig. 6 is the structural representation of sequencing reaction small chamber in the fifth embodiment of the invention.
Fig. 7 is the structural representation of microfluid component in the sixth embodiment of the invention.
Fig. 8 is another structural representation of microfluid component in the sixth embodiment of the invention.
Fig. 9 is the structural representation of mechanical manipulator in the seventh embodiment of the invention.
Embodiment
In order to make the object of the invention, technical scheme and advantage clearer,, the present invention is further elaborated below in conjunction with accompanying drawing and embodiment.
Point sample instrument among the present invention is the device that is used for sequencing reaction small chamber is carried out point sample, also is about to testing sample and is fixed on the device in the sequencing reaction small chamber.Testing sample can directly be fixed in the sequencing reaction small chamber, also can be fixed in the sequencing reaction small chamber through magnetic bead.Sequencing reaction small chamber through behind the point sample is fixed in the detection of nucleic acids appearance, carries out sequencing reaction; Then, order-checking is adopted map device figure is adopted in the sequencing reaction zone in the sequencing reaction small chamber; At last, the information of adopting figure is handled the information of the testing sample that becomes.Wherein, described testing sample includes but not limited to nucleic acid samples, can be dna fragmentation, RNA fragment, also can be dna fragmentation or the RNA fragment that is fixed on the magnetic bead.
The present invention proposes first embodiment, and a kind of point sample instrument comprises control unit, microfluid component and sequencing reaction small chamber.Fig. 1 has provided the structural representation of this point sample instrument, is elaborated in the face of this point sample instrument down.
(1) control unit 1, control microfluid component 2 transmission reagent.
Said control unit 1 can comprise computingmachine; Such as: programmable logic controller (Programmable Logic Controller; PLC), Personal Computer (personal computer; PC) etc., do not have particular restriction, all can be used as control unit 1 of the present invention as long as possess the device or the equipment of function for control unit.Control unit 1 in the present embodiment can possess the function that receives extraneous input order; The dosage of the kind of the reagent that the user needs through control unit 1 input microfluid component 2 to extract, every kind of reagent needing to extract, extract the instruction of the speed etc. of reagent; Control unit 1 becomes steering order with the instruction transformation of input, the work of control microfluid component 2.Control unit 1 in the present embodiment also can be according to the work of the instruction control microfluid component of giving tacit consent to 2.
(2) microfluid component 2, are used for reagent is transferred to sequencing reaction small chamber 3.
Said microfluid component 2 extracts reagent under the control of control unit 1, and reagent is transferred in the sequencing reaction small chamber 3.Microfluid component 2 can extract reagent or the extraction reagent from the outside from point sample instrument is inner.Wherein microfluid component 2 can be connected through conduit with sequencing reaction small chamber 3.
(3) sequencing reaction small chamber 3, comprise at least one point sample chamber.
Said sequencing reaction small chamber 3 can be the sequencing reaction small chamber 3 in any high-throughput nucleic acid proofing unit.This sequencing reaction small chamber 3 can be the sequencing reaction small chamber 3 with single passage or hyperchannel point sample chamber; Also can be single sequencing reaction small chamber 3 or a plurality of sequencing reaction small chamber 3; When a plurality of sequencing reaction small chamber 3, separate between the different sequencing reaction small chambers 3, be independent of each other.During point sample, microfluid component 2 can be simultaneously carries out independent point sample to each point sample chamber of the sequencing reaction small chamber 3 with hyperchannel point sample chamber, also can carry out point sample to each point sample chamber successively.Equally, microfluid component 2 can carry out independent point sample to a plurality of sequencing reaction small chamber 3 simultaneously, also can carry out independent point sample to each sequencing reaction small chamber 3 successively.Among the present invention, the point sample of each sequencing reaction small chamber 3 there is not particular restriction, as long as can realize point sample.In the present embodiment, the surface of sequencing reaction small chamber 3 can combine beads or DNA or RNA fragment through physics or chemical treatment.Preferably, sequencing reaction small chamber 3 is handled through amination and/or hydroxylation.
Provide a concrete example at this, the instruction through control unit 1 input is: Beads (magnetic bead) solution, 30 μ L, 0.02 μ L/S (microlitre/second); Control unit 1 becomes steering order with the instruction transformation of input, microfluid component 2 according to steering order from the position at Beads solution place with speed be 0.02 μ L/S extract 30 μ L Beads solution to sequencing reaction small chamber 3.In this example; The density of supposing Beads solution is 1,000 ten thousand/μ L (1 μ L has 1,000 ten thousand magnetic beads); Beads solution is pumped into sequencing reaction small chamber slowly through microfluid component 2; And filling up whole order-checking reaction chamber, the Beads in the Beads solution fully contacts through physics or chemically treated surface with sequencing reaction small chamber 3, and fully reaction.If the volume of sequencing reaction small chamber is 75cm*40cm*0.1cm, when Beads solution surpasses 2 minutes in the time that sequencing reaction small chamber stops, then the point sample density on sequencing reaction small chamber surface can reach 50,000/cm
2
In the technique scheme of present embodiment, control unit 1 control microfluid component 2 accurately extracts reagent, thereby realizes the full-automation to sequencing reaction small chamber 3 point samples.Simultaneously, the microfluid component 2 of the point sample instrument in the present embodiment is communicated with through conduit with sequencing reaction small chamber 3, when extracting reagent; Reagent is from getting into microfluid component 2; Enter into sequencing reaction small chamber 3 through conduit again, all do not contact, thereby reduced the influence of air reagent with the external world; In addition, reagent is full of the point sample chamber of whole sequencing reaction small chamber, thereby makes reagent be evenly distributed in the sequencing reaction small chamber, has realized uniform point sample, has also promptly realized high-quality point sample.Make that finally follow-up sequencing quality is higher.To the microfluid component among first embodiment, the present invention provides a concrete example, and when microfluid component extracted reagent internally to sequencing reaction small chamber, microfluid component can comprise pumping device and liquid storage device.As shown in Figure 2, pumping device 201 is used for extracting reagent from liquid storage device 202; Liquid storage device 202 is used to store the required reagent of point sample instrument point sample.
In the present technique scheme, microfluid component has been realized the long-time storage of reagent, can once add more reagent, avoids repeating to add or change the trouble of reagent.
In the technique scheme, sequencing reaction small chamber is provided with gauge point, and the position of this gauge point is not limit, can be in the optional position of sequencing reaction small chamber.Preferably, this gauge point be positioned at sequencing reaction small chamber and order-checking adopt map device over against the center of gravity place of face.After point sample is accomplished, it is mounted to carries out sequencing reaction in the detection of nucleic acids appearance, then; Order-checking is adopted map device to adopting figure in the point sample chamber; Because the detection of nucleic acids appearance is highly sophisticated device, claimed accuracy is very high, adopts figure so need adopt sequencing reaction small chamber or order-checking again after map device is accurately regulated; When sequencing reaction small chamber is provided with gauge point; The position that map device is discerned this gauge point is automatically adopted in order-checking, thereby realizes that order-checking adopts being automatically moved to of map device or sequencing reaction small chamber and adopt the figure position, and also promptly order-checking is adopted map device and realized the automatic location to sequencing reaction small chamber.This gauge point is adopted map device through order-checking and is discerned the automatic location of realization to sequencing reaction small chamber automatically, has improved level of automation greatly.
Based on first embodiment, the present invention provides second embodiment, and is as shown in Figure 3, and point sample instrument comprises control unit, microfluid component, sequencing reaction small chamber and temperature control assembly.
(1) control unit 1, and control microfluid component 2 transmits reagent and controls the work of temperature control assembly.
(2) microfluid component 2, are used for reagent is transferred to sequencing reaction small chamber 3.
(3) sequencing reaction small chamber 3, comprise at least one point sample chamber.
(4) temperature control assembly 4 is connected with sequencing reaction small chamber 3, is used for sequencing reaction small chamber 3 is heated or freezes.
In the present embodiment, control unit 1 also is used to control the work of temperature control assembly 4, such as the heating or the refrigeration of control temperature control assembly 4.These temperature control assembly 4 no particular restriction.Preferably, this temperature control assembly 4 comprises cooling piece or heating piece.When control unit 1 control microfluid component 2 is pumped into sequencing reaction small chamber 3 with reagent; 4 pairs of sequencing reaction small chambers of control unit 1 control temperature control assembly carry out temperature control; Thereby the reagent in the realization sequencing reaction small chamber 3 and the internal surface of sequencing reaction small chamber 3 fully react; Making that the point sample quality is higher, also is that the homogeneity of point sample is better.
To first or second embodiment, the present invention proposes the 3rd embodiment.As shown in Figure 4, said sequencing reaction small chamber can comprise a plurality of point samples chamber.Sequencing reaction small chamber 3 comprises a plurality of point samples chamber 31, has only provided the sequencing reaction small chamber that sequencing reaction small chamber 3 comprises 3 point sample chambeies 31 among the figure, and the structure that other comprise the sequencing reaction small chamber 3 in a plurality of point samples chamber 31 do not illustrate in the present invention one by one.Preferably, sequencing reaction small chamber 3 comprises 1 ~ 16 point sample chamber 31, and further preferred, sequencing reaction small chamber 3 comprises 2 ~ 8 point sample chambeies 31.The shape in the point sample chamber 31 in the present embodiment is not limit, and only having provided these point sample chamber 31 sections among Fig. 4 is rectangular structure, but this figure does not limit the shape in the point sample chamber 31 among the present invention.The sectional view in the point sample chamber 31 among the present invention can be rectangle, ellipse, circle, square, wicker leaf shape and irregularly shaped etc.
This programme is changed the point sample chamber as required, not only makes point sample can reach the flux of expectation, does not waste reagent again, and makes reagent fully to contact with sample, improves the quality of sample and reagent react.
Below will do further detailed description to point sample chamber 31 in the present embodiment, shown in 4-1 among Fig. 4 and 4-2, point sample chamber 31 comprises reagent inlet 310, reagent outlet 314 and point sample zone 312.Wherein:
(1) reagent inlet 310 is used to receive the reagent of microfluid component transmission.
Reagent gets into point sample chamber 31 from reagent inlet 310, thereby is full of whole point sample chamber 31.Microfluid component can be connected through conduit with the reagent inlet 310 of sequencing reaction small chamber.Reagent inlet 310 does not have particular restriction with the position of reagent outlet 314.Preferably, shown in 4-1 among Fig. 4, reagent inlet 310 lays respectively on 312 opposite faces of point sample zone with reagent outlet 314, thereby can avoid diffusion barrier so that reagent gets into after the point sample chamber 31, realizes that reagent and sample fully react.Further preferred, shown in 4-2 in 4, reagent inlet 310 is positioned at the opposite end of point sample zone 312 opposite faces with reagent outlet 314, thereby makes reagent can be full of whole sequencing reaction small chamber.
(2) reagent outlet 314 is used for the interior reagent of output sequencer reaction small chamber.
The reagent in the sequencing reaction small chamber is discharged in said reagent outlet 314, the reagent when it not only is used to discharge point sample in the sequencing reaction small chamber, the reagent when also being used to discharge order-checking in the sequencing reaction small chamber.Wherein, reagent inlet 314 can exchange with reagent outlet 312, and when one entered the mouth for reagent, another was reagent and exports.
(3) point sample zone 312 is used to combine magnetic bead or direct fixed dna or RNA fragment.
Said point sample zone 312 is through chemically treated.Preferably, this point sample zone 312 is handled through hydroxylation or amination is handled, and can combine magnetic bead or combine the DNA/RNA fragment.Point sample is that DNA/ or RNA are fixed in the point sample zone 312, and the point sample zone 312 in the present embodiment can combine magnetic bead (magnetic bead can combine DNA or RNA fragment) perhaps directly to combine with DNA or RNA fragment through after the chemical treatment, thereby realizes point sample.The area in this point sample zone 312 is unsuitable excessive, and when point sample zone 312 areas were excessive, map device is adopted in order-checking can't collect the image in the whole point sample zone 312, thus all images data after can't obtaining to check order.Preferably, the area in this point sample zone 312 is preferably at 3500mm
2Below.
How many technical schemes in the present embodiment, sequencing reaction small chamber can according to the changing of point sample, and the number in point sample chamber also can select as required, neither waste point sample reagent and make that also the point sample area is moderate, thereby realized the high quality point sample.
Based on above-mentioned first, second or the 3rd embodiment, the present invention proposes the 4th embodiment.As shown in Figure 5, above-mentioned sequencing reaction small chamber can comprise diaphragm seal 33 and the deckglass 32 that parallel carries print 34, is provided with hole logical in a plurality of.Below sequencing reaction small chamber 3 is elaborated.
(1) carries print 34 and be used for fixing DNA or RNA fragment.
Print 34 was through chemical treatment, directly or indirectly fixed dna or RNA fragment in said year.Carry print 34 and serve as reasons arbitrarily through chemical treatment, for example hydroxylation is handled or amination is handled, and can combine the segmental material of magnetic bead or dna fragmentation or RAN to process.Preferably, this year, print 34 material was simple glass or toughened glass.
(2) diaphragm seal 33 is used for being divided into isolated area with carrying print 34.
Said diaphragm seal 33 be provided with a plurality of in logical hole.Hole logical in a plurality of combines with a year print 34, will carry print 34 and be divided into a plurality of point samples zone.Sealing sheet 33 material with sealing function of serving as reasons is processed.The thickness of sealing sheet 33 with in the size in logical hole determined the point sample flux of sequencing reaction small chamber.The thickness of sealing sheet 33 does not have particular restriction, preferably between 0.05mm ~ 2mm.The size of the area in logical hole does not have particular restriction yet in being somebody's turn to do, preferably at 3000mm
2Below.
(3) deckglass 32 is used for forming the point sample chamber with carrying print 34 with diaphragm seal 33.
Said deckglass 32 is provided with perforate, and they are corresponding to the optional position in hole logical in the diaphragm seal 33, and the corresponding deckglass 32 in hole logical in each is provided with two perforates, thereby forms reagent outlet and reagent inlet.The material of this deckglass 32 does not have particular restriction, and preferred, the material of deckglass 32 is simple glass or toughened glass or silica gel.
In the present technique scheme, the gauge point that sequencing reaction small chamber is provided with, the position of this gauge point is not limit.Preferably, this gauge point is positioned at deckglass or carries the optional position on the print.Further preferred, this gauge point is positioned at deckglass or carries on the center of gravity of print.
Year print, diaphragm seal and the deckglass of the sequencing reaction small chamber in the present embodiment are three-decker; Can dismantle, through cleaning, can reuse after using; Also be that sequencing reaction small chamber of the present invention can be reused, thereby reduced the use cost of point sample instrument.
Based on first, second or the 3rd embodiment, the present invention proposes the 5th embodiment, and is as shown in Figure 6.Sequencing reaction small chamber comprises at least one point sample chamber, and preferred, sequencing reaction small chamber comprises 2 ~ 16 point sample chambeies.Said point sample chamber comprises the chamber 311 and the point sample zone 312 of reagent inlet 310, reagent outlet 314, hollow.Preferably; Point sample chamber in the present technique scheme comprises the chamber 311 of a hollow; Wherein one side in the point sample chamber can be used for combining DNA or RNA fragment or magnetic bead through special processing, also is point sample zone 312; The optional position of the relative face in any two relative faces or point sample zone 312 has hole logical in two in the four sides adjacent with point sample zone 312, forms reagent inlet 310 and reagent outlet 314.Reagent inlet 310 receives the reagent that the microfluid components transmission comes, and the chamber 311 of whole hollow is filled up in the reagent point sample zone 312 of flowing through, and flows out from reagent outlet 314.Said point sample zone 312 can be used for combining DNA or RNA fragment or magnetic bead through special processing.The size in said point sample zone 312 has determined the flux in point sample chamber, according to the size that the flux of area of pictural surface size and needs point sample of map device is adopted in order-checking, can select different point sample chambeies.In the present technique scheme, the transparency in point sample chamber does not have particular restriction, and is preferred, and the face and the point sample zone 312 relative faces at 312 places, point sample zone in point sample chamber are that transparent material is processed, like simple glass, toughened glass etc.In the present technique scheme, the gauge point that sequencing reaction small chamber is provided with, the position of this gauge point is not limit.Preferably, this gauge point is positioned at the face outside at 312 places, point sample zone or the outside of the face relative with it.
The good airproof performance of the sequencing reaction small chamber in this technical scheme liquid can not occur and omit, and simultaneously, this sequencing reaction small chamber as a whole need not assembling when using, improved the ease for use of sequencing reaction small chamber greatly.
Based on above-mentioned arbitrary embodiment, the present invention proposes the 6th embodiment.As shown in Figure 7, above-mentioned microfluid component can comprise pump 21 and containing liquid chamber 22.
(1) pump 21 is connected with sequencing reaction small chamber with containing liquid chamber 22 respectively, is used for the reagent of containing liquid chamber 21 is transferred to the point sample chamber in the sequencing reaction small chamber.
The liquor pump of said pump 21 for selling on the market.Preferably, this pump 21 can be used for lashing simultaneously the liquor pump of various liquid.Further preferred, this pump 21 is for having the liquor pump 21 of nine hole pumps.
(2) at least a reagent of containing liquid chamber 22 storages.
But at least a reagent of said containing liquid chamber 22 separate, stored.When the storage plurality of reagents, this plurality of reagents can be the same or different.
In the present technique scheme, when sequencing reaction small chamber had a point sample chamber, pump 21 was for being used for taking out/beating the pump of liquid arbitrarily.When sequencing reaction small chamber had a plurality of point samples chamber, the point sample chamber can be put similar and different sample respectively.During to the parallel independent point sample in point sample chamber; Preferably; Pump 21 can be the porous pump, also is that pump 21 has a plurality of holes and the hole of beating liquid that are used for the drawing liquid body, and different reagent can be extracted through conduit respectively in the hole of said drawing liquid body from containing liquid chamber 22; The said hole of beating liquid can be connected with the point sample chamber through conduit respectively, is used for the reagent that extracts independently is transferred to different point sample chambeies.When point sample is carried out successively in the point sample chamber; Pump 21 no particular requirements as long as the function of taking out/beating liquid is arranged, also are that pump 21 can extract a kind of reagent in the containing liquid chamber 22 through conduit; And reagent is driven in the point sample chamber through conduit; Extract the another kind of reagent in the containing liquid chamber 22 through conduit again, and another kind of reagent is driven in other the point sample chamber through conduit, till accomplishing required point sample.
But microfluid component skimmer 23 also is as shown in Figure 8 among the 6th embodiment.
(1) pump 21 is connected with skimmer 23 with containing liquid chamber 22 respectively, is used for the reagent of containing liquid chamber 21 is transferred in the skimmer 23.
Said pump 21 is for can be used for taking out/beating the pump of liquid arbitrarily.Pump extracts reagent through conduit from containing liquid chamber 22, and through conduit reagent is transferred in the skimmer 23.
(2) containing liquid chamber 22 can be used for storing at least a reagent.
Said containing liquid chamber 22 can store one or more reagent.Pump 21 can be connected with containing liquid chamber 22 through one or more conduit.When pump 21 is the porous pump, can there be a plurality of drawing liquids hole to be connected with containing liquid chamber 22 through conduit.
(3) skimmer 23 is connected with the point sample chamber, is used to receive the reagent of pump 21 transmission, and the reagent that receives is divided equally and imported to each point sample chamber.
The inlet of said skimmer 23 is connected with pump 21 through conduit, receive the reagent of pump 21 transmission after, the outlet of skimmer 23 is passed through conduit and is connected with the point sample chamber.Reagent can be divided equally through behind the skimmer 23, and the reagent after dividing equally is directed in the different point sample chambeies.The outlet number of skimmer 23 does not have particular restriction.The outlet number that can select corresponding skimmer 23 to launch according to the number in point sample chamber.When N point sample chamber, the outlet that skimmer 23 is launched can have N, when reagent when dividing the outlet of sap cavity 23, import to respectively in N the point sample chamber after being divided into the N equal portions.The outlet of not enabled is sealed in the skimmer 23, and liquid that can not flows out from this outlet.
In the present technique scheme, pump 21 and skimmer 23 are connected through conduit, and when skimmer 23 had only an inlet, an outlet of pump 21 was connected with the inlet of skimmer 23, and reagent is transferred in the skimmer 23; When skimmer 23 has a plurality of inlet; Also be five equilibrium and the transmission that skimmer 23 can independently carry out plurality of reagents; Can select to have the pump 21 (a plurality of outlets of pump 21 be used for beat reagent) of a plurality of outlets, the outlet of pump 21 is connected with the inlet of skimmer 23, is used for the different reagent of independent transmission.This technical scheme of the present invention can improve the efficient of having put the transmission of appearance appearance reagent greatly with importing to rapidly and uniformly with a kind of reagent in a plurality of point samples chamber, thereby improves the working efficiency of point sample instrument.
Based on the 6th embodiment, the present invention proposes the 7th embodiment.Microfluid component also can comprise mechanical manipulator.Said mechanical manipulator is connected with pump, and the reagent that is used for extracting containing liquid chamber is in pump.In the present technique scheme, the number of said mechanical manipulator can be for one or more, and when mechanical manipulator when being a plurality of, mechanical manipulator is respectively applied for the different reagent that extract in the containing liquid chamber in pump, make non-interfering work between the mechanical manipulator.Mechanical manipulator is connected with the drawing liquid hole of pump, and pump is that mechanical manipulator provides the power that extracts reagent.Mechanical manipulator is transferred to reagent in the pump through conduit after being drawn into the corresponding reagent in the containing liquid chamber.Also be that pump can obtain reagent or directly from containing liquid chamber, extract acquisition reagent through conduit through mechanical manipulator.Wherein, the moving of control unit may command mechanical manipulator, mechanical manipulator accurately moves to the position at reagent place in the containing liquid chamber, and the control unit control pump extracts reagent, thereby for mechanical manipulator provides the power that extracts reagent, realizes that mechanical manipulator extraction liquid is in pump.
In the present technique scheme; Mechanical manipulator can accurately extract multiple reagent; Thereby avoided when the kind of the reagent that needs to extract is too much, needing the annexation of the reagent of artificial adjustment pump and containing liquid chamber, simultaneously; Also can further improve the precision that reagent extracts, improved the level of automation and the precision that extracts reagent of point sample instrument on the whole.
Fig. 9 is the synoptic diagram of mechanical manipulator among the 7th embodiment, and this mechanical manipulator comprises: three-dimensional motion mechanism 241 and imbibition syringe needle 242.
(1) imbibition syringe needle 241 is fixed on the mechanical arm, is used for extracting the reagent of containing liquid chamber.
Said imbibition syringe needle 241 can be drawn the device of reagent for the power of the extraction liquid that provides through pump arbitrarily, does not have any particular restriction, for example syringe needle.Preferably, said imbibition syringe needle 241 is a rubber-like imbibition syringe needle, when imbibition syringe needle 241 contacts the bottom of containing liquid chamber, because elasticity makes this imbibition syringe needle not be damaged.Preferably, the hole of said imbibition syringe needle 241 is an inclined hole, has effectively prevented can't extract reagent when imbibition syringe needle 241 from contacting the bottom of containing liquid chamber.
(2) three-dimensional motion mechanism 242 is used to drive imbibition syringe needle 241 and moves.
The device of said three-dimensional motion mechanism 242 on three-dimensional space, moving arbitrarily do not have any particular restriction.When needs extract certain liquid; 242 motions of control unit control three-dimensional motion mechanism; When move to the reagent that need to extract be positioned at imbibition syringe needle 241 under the time, the drawing liquid hole of control unit control pump is extracted, pump is given imbibition syringe needle 241 with the transmission of power of the extraction liquid in drawing liquid hole; Imbibition syringe needle 241 extracts reagent, and reagent is transferred in the pump through conduit.
In the present technique scheme; Three-dimensional motion mechanism 242 can stop the optional position above containing liquid chamber; Extract any agent in the containing liquid chambers for imbibition syringe needle 241, guaranteed that point sample instrument can the different reagent of flexible automatic extraction, improved the level of automation of instrument greatly.
Should be noted that typical application of the present invention but be not limited to point sample instrument itself, in other the similar structures that in the precision engineering field, also can use point sample instrument that the present invention sets forth.
The above is merely preferred embodiment of the present invention, not in order to restriction the present invention, all any modifications of within spirit of the present invention and principle, being done, is equal to and replaces and improvement etc., all should be included within protection scope of the present invention.
Claims (10)
1. a point sample instrument is characterized in that, comprises control unit, microfluid component and sequencing reaction small chamber;
Said control unit control microfluid component transmission reagent;
Said microfluid component is used for reagent is transferred to sequencing reaction small chamber;
Said sequencing reaction small chamber comprises at least one point sample chamber;
The internal surface of said sequencing reaction small chamber is through physics or chemical treatment.
2. point sample instrument according to claim 1 is characterized in that said sequencing reaction small chamber is provided with gauge point; Said gauge point is used to check order and adopts the automatic location of map device to sequencing reaction small chamber.
3. point sample instrument according to claim 2 is characterized in that, also comprises the temperature control assembly;
Said temperature control assembly is connected with sequencing reaction small chamber, is used for sequencing reaction small chamber is heated or freezes;
Said control unit also is used to control the work of temperature control assembly.
4. point sample instrument according to claim 2 is characterized in that, said each point sample chamber includes reagent inlet, reagent outlet and point sample zone;
Said reagent inlet is used to receive the reagent of microfluid component transmission;
Said reagent outlet is used for the reagent in the output sequencer reaction small chamber;
Said point sample zone is handled through hydroxylation or amination is handled, and is used to combine magnetic bead or DNA or RNA fragment.
5. point sample instrument according to claim 4 is characterized in that, said sequencing reaction small chamber comprises the parallel diaphragm seal that carries print, deckglass and be provided with hole logical in a plurality of;
Said diaphragm seal forms a plurality of point samples chamber jointly carrying between print and the deckglass.
6. point sample instrument according to claim 5 is characterized in that, print was divided into a plurality of point samples zone by diaphragm seal in said year.
7. point sample instrument according to claim 5 is characterized in that, said gauge point is positioned at and carries on print or the deckglass.
8. according to each described point sample instrument in the claim 1 to 7, it is characterized in that said microfluid component comprises pump and containing liquid chamber;
Said pump is connected with containing liquid chamber, is used for the reagent of containing liquid chamber is transferred to the point sample chamber in the sequencing reaction small chamber;
Said containing liquid chamber is stored at least a reagent.
9. point sample instrument according to claim 8 is characterized in that said microfluid component also comprises skimmer;
Said skimmer is connected with the point sample chamber with pump respectively, is used to receive the reagent of pump transmission, and the reagent that receives is divided equally and imported to each point sample chamber.
10. point sample instrument according to claim 8 is characterized in that said microfluid component also comprises mechanical manipulator; Said mechanical manipulator is connected with pump, and the reagent that is used for extracting containing liquid chamber is in pump.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012102550539A CN102787068A (en) | 2012-07-23 | 2012-07-23 | Sample applicator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012102550539A CN102787068A (en) | 2012-07-23 | 2012-07-23 | Sample applicator |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102787068A true CN102787068A (en) | 2012-11-21 |
Family
ID=47152660
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012102550539A Pending CN102787068A (en) | 2012-07-23 | 2012-07-23 | Sample applicator |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102787068A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106434317A (en) * | 2015-10-16 | 2017-02-22 | 西安天隆科技有限公司 | Fluid sample processing device for biochemical reaction |
| CN109370900A (en) * | 2018-12-19 | 2019-02-22 | 深圳麦芽加速器科技有限公司 | A kind of gene sequencer |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102321743A (en) * | 2011-08-04 | 2012-01-18 | 盛司潼 | Solid-phase carrier for surface fixation and preparation and application methods for solid-phase carrier |
| CN102517206A (en) * | 2011-12-31 | 2012-06-27 | 盛司潼 | Gene sequencing device and system |
| CN102517196A (en) * | 2011-12-31 | 2012-06-27 | 盛司潼 | Multi-channle sequencing reaction chamber and gene sequencing instrument |
-
2012
- 2012-07-23 CN CN2012102550539A patent/CN102787068A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102321743A (en) * | 2011-08-04 | 2012-01-18 | 盛司潼 | Solid-phase carrier for surface fixation and preparation and application methods for solid-phase carrier |
| CN102517206A (en) * | 2011-12-31 | 2012-06-27 | 盛司潼 | Gene sequencing device and system |
| CN102517196A (en) * | 2011-12-31 | 2012-06-27 | 盛司潼 | Multi-channle sequencing reaction chamber and gene sequencing instrument |
Non-Patent Citations (1)
| Title |
|---|
| 陈克勇等: "工业光学检测中定位点的识别", 《计算机应用》, vol. 27, no. 1, 1 June 2007 (2007-06-01), pages 431 - 433 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106434317A (en) * | 2015-10-16 | 2017-02-22 | 西安天隆科技有限公司 | Fluid sample processing device for biochemical reaction |
| CN109370900A (en) * | 2018-12-19 | 2019-02-22 | 深圳麦芽加速器科技有限公司 | A kind of gene sequencer |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20220372423A1 (en) | Systems and methods for cell culture device interconnection and fluidic device interconnection | |
| JP6930050B2 (en) | Microincubation systems and methods for cell culture of microfluidics | |
| CN101500694B (en) | Droplet manipulation systems | |
| US10470379B1 (en) | High-throughput large-scale plant phenotyping instrumentation | |
| CN100394184C (en) | Micro-fluid transfer and analysis system | |
| CN104487562B (en) | Biochemical treatment apparatus | |
| CN104946510B (en) | Collection nucleic acid amplification and microarray are detected on the micro fluidic device of one | |
| US20100300563A1 (en) | Modular device and method for moving fluids to and from a sample delivery element | |
| CN111197003B (en) | Integrated nucleic acid extraction amplification detection analysis device and method based on smart phone | |
| CN101520034A (en) | Integrated normal-closed PDMS micro-valve, preparation process thereof and micro-pump containing micro-valve | |
| US10246675B2 (en) | Biochemical cartridge, and biochemical cartridge and cartridge holder set | |
| Liu et al. | A power-free, parallel loading microfluidic reactor array for biochemical screening | |
| Dettinger et al. | Open-source personal pipetting robots with live-cell incubation and microscopy compatibility | |
| CN103308502B (en) | Handheld general microfluidic chip real-time detection device and application | |
| CN102787068A (en) | Sample applicator | |
| CN207611061U (en) | A kind of micro-fluidic chip sampling device based on over-pressure control | |
| CN103352002B (en) | Gene amplification and gene test integral system | |
| US20200158744A1 (en) | Device for processing a liquid sample | |
| US20230039793A1 (en) | Sample Preparation Device and Methods for Using Same | |
| CN100504389C (en) | Transmission through applied microflow liquid, and method of analyzing system | |
| US20190201896A1 (en) | Microscale sampling device | |
| TWI671515B (en) | Flow control device and system containing the same | |
| Dettinger | Fluidic automation methods for dynamic cell culture interventions during time-lapse microscopy | |
| CN113512494A (en) | Cell micro-fluidic culture chip | |
| CN102681454A (en) | Biochemical reaction workstation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20121121 |