CN102779222A - Method for detecting bioavailable particulate phosphori in shallow lake - Google Patents
Method for detecting bioavailable particulate phosphori in shallow lake Download PDFInfo
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- CN102779222A CN102779222A CN2011101229902A CN201110122990A CN102779222A CN 102779222 A CN102779222 A CN 102779222A CN 2011101229902 A CN2011101229902 A CN 2011101229902A CN 201110122990 A CN201110122990 A CN 201110122990A CN 102779222 A CN102779222 A CN 102779222A
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Abstract
The invention discloses a method for detecting bioavailable particulate phosphori in a shallow lake. According to the method, an alga available phosphorus content and a total phosphorus content in suspended solids are detected, a percentage of alga available phosphori and total phosphori is used for representing the biological effectiveness of the particulate phosphori, and a bioavailable particulate phosphorus content can be expressed by the product of the percentage and the particulate phosphorus content in a water body. The determination of the particulate phosphori in the water body can be achieved by means of an indirect method, namely, the total phosphorus content and a solubility phosphate content in the water body are detected, and the difference between the total phosphorus content and the solubility phosphate content is regarded as the particulate phosphorus content. Compared with prior methods for detecting the bioavailable particulate phosphori, the method for detecting the particulate phosphori in the shallow lake is more suitable for detecting the particulate phosphori in the shallow lakes or shallow water bodies which are prone to produce bottom mud suspending; and the method has the advantages of being rapid, capable of directly quantifying, easy to operate and the like, and by means of the method, the detecting results which are obtained in different water bodies by different people are referential and repeatable.
Description
Technical field
The invention belongs to the environmental improvement technical field, being specifically related to a kind of the detection can be by the method for biological utilisation particulate form phosphorus in the shallow lake.
Background technology
Phosphorus is the restrictive factor of body eutrophication.At present, the research that the migration of phosphorus transforms in the relevant water body is a lot of, and the detection method of phosphorus is also numerous, mainly concentrates on two parts in water body and the bed mud.
At present, in the national standard method, the detection method of total phosphorus and soluble phosphate is comparatively ripe.Domestic and international academic article then is more common in detection for endogenous phosphorus in the bed mud.A kind of about can then only being had by the direct detecting method of biological utilisation particulate form phosphorus content (BAPP) in the water body, i.e. bioanalysis.This method is that calculate through the growth of microcystic aeruginosa of record " phosphate starvation " can be by the content of biological utilisation particulate form phosphorus, but this method since the time long (14 days) use less, nor the fast measuring of suitable big batch of sample.
At present, also having some to measure through indirect mode can be by the method for biological utilisation particulate form phosphorus.Comprise iron oxide impregnated paper method, the compound embrane method of iron oxide/acetate fiber and resin anion (R.A.) method.This several method all is through measuring biological available phosphorus content (BAP) in the water body, then, therefrom deducts dissolved phosphorus content (DTP) and obtain can be by biological utilisation particulate form phosphorus content (BAPP).But; The shortcoming of this several method is all clearly: in the compound embrane method of iron oxide impregnated paper method and iron oxide/acetate fiber; Applied iron oxide impregnated paper and iron oxide/acetate fiber composite membrane all needs preparation voluntarily; This causes same batch filter paper or result that composite fiber membrane is surveyed to have certain comparability, different batches then do not possess comparability, so these two kinds of methods are all extensively promoted.The shortcoming of resin anion (R.A.) method is to the non-selectivity of anionic adsorption/desorption, and can not keep low phosphorus and serve as infinitely " remittance ".
Thus it is clear that, can also do not had a kind of method simple, that be prone to row directly to detect by biological utilisation particulate form phosphorus content as biological available phosphorus content important component part in the water body.
Summary of the invention
For overcoming deficiency of the prior art; The objective of the invention is to propose a kind of the detection can be by the method for biological utilisation particulate form phosphorus in the shallow lake; Having solved in the existing method fast direct quantitative measures and can also have been solved the not strong problem of measuring in the existing method of result comparison simultaneously by the problem of biological utilisation particulate form phosphorus.
For solving the problems of the technologies described above, reach above-mentioned technical purpose, the present invention adopts following technical scheme:
A kind of detection in the shallow lake of the present invention can be may further comprise the steps by the method for biological utilisation particulate form phosphorus:
The biological effectiveness PP% of step 1) count particles attitude phosphorus: measure phosphorus content AAP capable of using and the total phosphorus content Tot-P of algae in the suspension, the biological effectiveness PP% of count particles attitude phosphorus, the biological effectiveness PP%=AAP/Tot-P of particulate form phosphorus PP;
Step 2) the content PP of count particles attitude phosphorus: measure total phosphorus content TP and soluble phosphate content DIP in the water body, the content PP=TP-DIP of particulate form phosphorus;
Step 3) is calculated can be by the content BAPP of biological utilisation particulate form phosphorus: can be by the content BAPP=(AAP/Tot-P) * (TP-DIP) of biological utilisation particulate form phosphorus.
In shallow lake, phosphorus is the restricted factor of body eutrophication, and its detection method is a lot of; But be directed against eutrophication, in the water body can by the phosphorus of biological utilisation comprise dissolved phosphorus with can be by the particulate form phosphorus of biology usefulness, wherein; The former detection is more or less freely, and method is ripe; But the latter wants fast, direct quantitative is measured comparatively difficulty.Suspension content is closely related in particulate form content and the water body, particularly in being easy to take place the shallow lake that bed mud suspends, therefore, can be closely related by the biological effectiveness of biological utilisation particulate form phosphorus and particulate form phosphorus.Based on this, can come quantitative description particulate form phosphorus biological effectiveness from the angle of the release characteristics of endogenous phosphorus.From the angle that endogenous phosphorus discharges, weak ADSORPTION STATE phosphorus and iron aluminium combined state phosphorus all belong to this category, but owing to iron aluminium combined state phosphorus can not all discharge, and only have non-closing to hold attitude phosphorus and can discharge.Non-closing held attitude iron aluminium combined state phosphorus and can be adopted algae attitude phosphorus content capable of using to represent.Consider the assay method of algae attitude phosphorus content capable of using; Therefore; The number percent that accounts for total phosphorus content with algae attitude phosphorus content capable of using can be represented the biological effectiveness of particulate form phosphorus, promptly has so many phosphorus to discharge in the particulate form phosphorus, and biological available phosphorus in the water body is had contribution.Its content then can adopt the content that this number percent multiply by particulate form phosphorus in the water body to calculate.
In the technique scheme, said water body is shallow lake or shallow type water body, under effects such as stormy waves, bottom-dwelling, artificial interference, is easy to take place bed mud and suspends.
With respect to scheme of the prior art, advantage of the present invention is:
1. can be compared with the former studies method by the method for biological utilisation particulate form phosphorus in the detection shallow lake of the present invention, have fast, characteristics such as direct quantitative, easy operating.In addition, detection method provided by the invention makes that had in the testing result of different water bodys by different people can be with reference to property, repeatability.
2. the present invention is suitable for being easy to take place shallow lake or the shallow type water body that bed mud suspends.
Below in conjunction with embodiment this patent is further described.
Embodiment
Below in conjunction with specific embodiment such scheme is further specified.Should be understood that these embodiment are used to the present invention is described and are not limited to limit scope of the present invention.The implementation condition that adopts among the embodiment can be done further adjustment according to actual conditions, and not marked implementation condition is generally the condition in the normal experiment.
Can be may further comprise the steps by the method for biological utilisation particulate form phosphorus in the detection shallow lake of the present invention:
The biological effectiveness PP% of step 1) count particles attitude phosphorus: measure algae phosphorus content AAP capable of using and total phosphorus content Tot-P in the suspension, the percentage of algae phosphorus content AAP capable of using and total phosphorus content Tot-P is recently represented particulate form phosphorus biological effectiveness;
Step 2) the content PP of count particles attitude phosphorus: measure total phosphorus content TP and soluble phosphate content DIP in the water body, with the difference of total phosphorus content TP and soluble phosphate content DIP as particulate form phosphorus content PP;
Step 3) is calculated can be by the content BAPP of biological utilisation particulate form phosphorus: can be the product of the content PP of particulate form phosphorus in biological effectiveness PP% and the water body of particulate form phosphorus by biological utilisation particulate form phosphorus content BAPP.
Can be in the water body of moon gulf, embodiment 1 Taihu Lake by the mensuration of biological utilisation particulate form phosphorus
Detecting in the water body of moon gulf, Taihu Lake with method of the present invention can be by biological utilisation particulate form phosphorus content, and compares with bioanalysis.The water sampling point be positioned at moon gulf (N31 ° 24 ' 38.8 ", E120 ° of 6 ' 4.57 ").Use some Plastic Drums, gather water sample 50L, take back the laboratory.Measure total phosphorus content TP and soluble phosphate content DIP in the water sample respectively, be respectively 0.065mg/L and 0.04mg/L, therefore, particulate form phosphorus content PP is 0.025mg/L.Adopt 0.45 μ m membrane filtration water sample, collect suspension.A part is used to measure algae and utilizes attitude phosphorus content AAP and total phosphorus content Tot-P, adopts different suspensions to detect 3 times, and the result representes with mean value ± mean square deviation.The algae phosphorus content AAP capable of using and the total phosphorus content Tot-P that gather suspension are respectively 149.04 ± 4.10mg/kg and 373.91 ± 30.41mg/kg.Particulate form phosphorus biological effectiveness then:
PP％＝AAP/Tot-P＝149.04/373.91＝39.95％±2.15％
Then adopt in the inventive method calculating water sample and can be by biological utilisation particulate form phosphorus content:
BAPP＝(AAP/Tot-P)*(TP-DIP)＝9.580±0.801μg/L
Another part adopts bioanalysis to measure can be by biological utilisation particulate form phosphorus content.
In 3 125mL conical flasks, add the wet deposition thing that 0.50g collects respectively, and add the without phosphorus nutrient solution of 50mL, add " phosphate starvation " microcystic aeruginosa (12d) subsequently, initial concentration is 1000cell/mL.Under 24 ± 2 ℃ of temperature, intensity of illumination 4300lm ± 10% condition, cultivate 14d, shaking table speed is 60rpm.Behind the 14d, measure amount of algae, calculate BAPP content through amount of algae.Result of calculation is:
BAPP＝10.254±1.857μg/L
The relative deviation scope is 3.89%-7.90%.
The foregoing description just is to let the one of ordinary skilled in the art can understand content of the present invention and enforcement according to this in order technical conceive of the present invention and characteristics to be described, to be its objective is, can not limit protection scope of the present invention with this.The variation or the modification of every equivalence that the essence of content has been done according to the present invention all should be encompassed in protection scope of the present invention.
Claims (1)
1. one kind is detected in the shallow lake and can be it is characterized in that by the method for biological utilisation particulate form phosphorus, may further comprise the steps:
The biological effectiveness PP% of step 1) count particles attitude phosphorus: measure algae phosphorus content AAP capable of using and total phosphorus content Tot-P in the suspension, the percentage of algae phosphorus content AAP capable of using and total phosphorus content Tot-P is recently represented particulate form phosphorus biological effectiveness;
Step 2) the content PP of count particles attitude phosphorus: measure total phosphorus content TP and soluble phosphate content DIP in the water body, with the difference of total phosphorus content TP and soluble phosphate content DIP as particulate form phosphorus content PP;
Step 3) is calculated can be by the content BAPP of biological utilisation particulate form phosphorus: can be the product of the content PP of particulate form phosphorus in biological effectiveness PP% and the water body of particulate form phosphorus by biological utilisation particulate form phosphorus content BAPP.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107014790A (en) * | 2017-03-07 | 2017-08-04 | 闽南师范大学 | A kind of method for detecting phytoplankton to copper complex bioavailability |
| CN109580514A (en) * | 2019-01-09 | 2019-04-05 | 水利部交通运输部国家能源局南京水利科学研究院 | A method of measuring abiotic particle cluster flow and biologic grain state phosphorus content |
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| US20030059465A1 (en) * | 1998-05-11 | 2003-03-27 | Unger Evan C. | Stabilized nanoparticle formulations of camptotheca derivatives |
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| US20030059465A1 (en) * | 1998-05-11 | 2003-03-27 | Unger Evan C. | Stabilized nanoparticle formulations of camptotheca derivatives |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107014790A (en) * | 2017-03-07 | 2017-08-04 | 闽南师范大学 | A kind of method for detecting phytoplankton to copper complex bioavailability |
| CN107014790B (en) * | 2017-03-07 | 2020-02-21 | 闽南师范大学 | Method for detecting bioavailability of copper complex by phytoplankton |
| CN109580514A (en) * | 2019-01-09 | 2019-04-05 | 水利部交通运输部国家能源局南京水利科学研究院 | A method of measuring abiotic particle cluster flow and biologic grain state phosphorus content |
| CN109580514B (en) * | 2019-01-09 | 2019-08-02 | 水利部交通运输部国家能源局南京水利科学研究院 | A method of measuring abiotic particle cluster flow and biologic grain state phosphorus content |
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