CN102779222B - A kind of detect in shallow lake can the method for bioavailable particle cluster flow - Google Patents

A kind of detect in shallow lake can the method for bioavailable particle cluster flow Download PDF

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Publication number
CN102779222B
CN102779222B CN201110122990.2A CN201110122990A CN102779222B CN 102779222 B CN102779222 B CN 102779222B CN 201110122990 A CN201110122990 A CN 201110122990A CN 102779222 B CN102779222 B CN 102779222B
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particle cluster
content
cluster flow
bioavailable
phosphorus
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CN102779222A (en
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李大鹏
黄勇
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Suzhou University of Science and Technology
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Suzhou University of Science and Technology
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Abstract

The invention discloses a kind of detect in shallow lake can the method for bioavailable particle cluster flow, by detecting the available phosphorus content of algae in float and total phosphorus content, the percentage ratio of available for algae phosphorus with total phosphorus is represented particle cluster flow biological effectiveness, can be represented with the product of particle cluster flow content in water body by this percentage ratio by bioavailable particle cluster flow content.In water body, particulate form phosphorus yield adopts indirect method, and namely by detecting total phosphorus in water content and soluble phosphate content, both differences are as particle cluster flow.In the detection shallow lake of the present invention can the method for bioavailable particle cluster flow mutually detection can the method for bioavailable particle cluster flow than ever, it is suitable for the shallow lake or the shallow water body that are prone to occur bed mud to suspend, have quickly, direct quantitative, the feature such as easily operated, different people is had the property of can refer to, repeatability in the testing result of different water bodys.

Description

A kind of detect in shallow lake can the method for bioavailable particle cluster flow
Technical field
The invention belongs to technical field of environmental management, being specifically related to a kind of detect in shallow lake can the method for bioavailable particle cluster flow.
Background technology
Phosphorus is the restrictive factor of body eutrophication.At present, a lot of about the research of the Transport And Transformation of phosphorus in water body, the detection method of phosphorus is also numerous, is concentrated mainly on water body and two parts in bed mud.
At present, in national standard method, total phosphorus is comparatively ripe with the detection method of soluble phosphate.Domestic and international academic article is then more common in for the detection of Endogenous Phosphorus in bed mud.About in water body can bioavailable particle cluster flow content (BAPP) direct detecting method then only have one, i.e. bioanalysis.The method be by record the growth of microcystic aeruginosa of " phosphate starvation " calculate can the content of bioavailable particle cluster flow, but the method is applied less due to time length (14 days), nor is suitable for the quick mensuration of big batch sample.
At present, also having some to be measured by indirectly mode can the method for bioavailable particle cluster flow.Including iron oxideimpregnated paper method, ferrum oxide/acetate fiber compound embrane method and resin anion (R.A.) method.This several method is all by measuring biologically effective phosphorus content (BAP) in water body, then, therefrom deducts dissolved phosphorus content (DTP) and obtain can bioavailable particle cluster flow content (BAPP).But, the shortcoming of this several method is all it is clear that in iron oxideimpregnated paper method and ferrum oxide/acetate fiber compound embrane method, the iron oxideimpregnated paper applied and ferrum oxide/acetate fiber composite membrane are both needed to prepare voluntarily, this filter paper causing same batch or composite fiber membrane measured result have certain comparability, different batches then do not possess comparability, so both of these approaches is all widely popularized.The shortcoming of resin anion (R.A.) method is that the adsorption/desorption to anion is non-specific, and can not maintain low phosphorus and serve as infinitely " remittance ".
Visible, as can directly the detecting but without a kind of simple, easy method by bioavailable particle cluster flow content of biologically effective phosphorus content important component part in water body.
Summary of the invention
For overcoming deficiency of the prior art, it is an object of the invention to propose a kind of detect in shallow lake can the method for bioavailable particle cluster flow, solve in existing method can not quickly directly quantitative assay can the problem of bioavailable particle cluster flow, also solve the problem that the result comparison measured in existing method is not strong simultaneously.
For solving above-mentioned technical problem, reaching above-mentioned technical purpose, the present invention adopts the following technical scheme that
The present invention a kind of detect in shallow lake can the method for bioavailable particle cluster flow, comprise the following steps:
Step 1) calculate the biological effectiveness PP% of particle cluster flow: measure the available phosphorus content AAP and total phosphorus content Tot-P of algae in float, calculate the biological effectiveness PP%, the biological effectiveness PP%=AAP/Tot-P of particle cluster flow PP of particle cluster flow;
Step 2) calculate the content PP of particle cluster flow: measure the total phosphorus content TP in water body and soluble phosphate content DIP, the content PP=TP-DIP of particle cluster flow;
Step 3) calculating can the content BAPP of bioavailable particle cluster flow: can content BAPP=(AAP/Tot-P) × (TP-DIP) of bioavailable particle cluster flow.
In shallow lake, phosphorus is the restricted factor of body eutrophication, and its detection method is a lot of, but for eutrophication, in water body can bioavailable phosphorus include dissolved phosphorus and can by the particle cluster flow of biology, wherein, the former detection is relatively easy to, and method is ripe;But the latter wants quickly, direct quantitative measures comparatively difficulty.Particulate form content and Suspended Matter in Water content are closely related, particularly occur in the shallow lake that bed mud suspends being prone to, therefore, can bioavailable particle cluster flow closely related with the biological effectiveness of particle cluster flow.Based on this, quantitative description particle cluster flow biological effectiveness can be carried out from the angle of the release characteristics of Endogenous Phosphorus.This category is broadly fallen into from the angle of internal phosphorus release, weakly stable state phosphorus and ferrum aluminum bound phosphorus, but owing to ferrum aluminum bound phosphorus can not all discharge, and only have the non-storage state phosphorus that closes and can discharge.The non-storage state ferrum aluminum bound phosphorus that closes can adopt the available state phosphorus content of algae to represent.Consider the assay method of the available state phosphorus content of algae, therefore, the percentage ratio accounting for total phosphorus content with the available state phosphorus content of algae can represent the biological effectiveness of particle cluster flow, namely has so many phosphorus to discharge in particle cluster flow, biologically effective phosphorus in water body is had contribution.Its content then can adopt the content that this percentage ratio is multiplied by water body particle cluster flow to calculate.
In technique scheme, described water body is shallow lake or shallow water body, is prone to occur bed mud to suspend under the effects such as stormy waves, benthon, artificial disturbance.
Relative to scheme of the prior art, the invention have the advantage that
1. in the detection shallow lake of the present invention can the method for bioavailable particle cluster flow, compared with conventional research method, have quickly, direct quantitative, the feature such as easily operated.It addition, detection method provided by the invention so that in the testing result of different water bodys, be there is the property of can refer to, repeatability by different people.
2. the present invention is suitable for being prone to shallow lake or the shallow water body that generation bed mud suspends.
Below in conjunction with detailed description of the invention, this patent is further described.
Detailed description of the invention
Below in conjunction with specific embodiment, such scheme is described further.Should be understood that these embodiments to be an illustration for the present invention and be not limited to restriction the scope of the present invention.The implementation condition adopted in embodiment can do further adjustment according to actual conditions, and not marked implementation condition is generally the condition in normal experiment.
In the detection shallow lake of the present invention can the method for bioavailable particle cluster flow, comprise the following steps:
Step 1) calculate biological effectiveness PP%: the available phosphorus content AAP and total phosphorus content Tot-P of algae in mensuration float of particle cluster flow, the percentage ratio of available for algae phosphorus content AAP and total phosphorus content Tot-P is represented particle cluster flow biological effectiveness;
Step 2) calculate the content PP of particle cluster flow: measure the total phosphorus content TP in water body and soluble phosphate content DIP, using the difference of total phosphorus content TP and soluble phosphate content DIP as particle cluster flow content PP;
Step 3) calculating can the content BAPP of bioavailable particle cluster flow: can bioavailable particle cluster flow content BAPP be biological effectiveness PP% and the product of the content PP of particle cluster flow in water body of particle cluster flow.
Can bioavailable particulate form phosphorus yield in the water body of moon gulf, embodiment 1 Taihu Lake
With the inventive method in detection moon gulf, Taihu Lake water body can bioavailable particle cluster flow content, and compare with bioanalysis.Water sampling point be positioned at moon gulf (N31 ° 24 ' 38.8 ", E120 ° 6 ' 4.57 ").Use some Plastic Drums, gather water sample 50L, take back laboratory.Measuring total phosphorus content TP and soluble phosphate content DIP, respectively 0.065mg/L and 0.04mg/L in water sample respectively, therefore, particle cluster flow content PP is 0.025mg/L.Adopt 0.45 μm of membrane filtration water sample, collect float.A part is used for measuring algae and utilizes state phosphorus content AAP and total phosphorus content Tot-P, adopts different float to detect 3 times, and result represents with meansigma methods ± mean square deviation.Gather the available phosphorus content AAP and total phosphorus content Tot-P respectively 149.04 ± 4.10mg/kg and 373.91 ± 30.41mg/kg of algae of float.Then particle cluster flow biological effectiveness:
PP%=AAP/Tot-P=149.04/373.91=39.95% ± 2.15%
Then adopt in the inventive method calculating water sample and can bioavailable particle cluster flow content be:
BAPP=(AAP/Tot-P) * (TP-DIP)=9.580 ± 0.801 μ g/L
Another part adopts bioanalysis to measure can bioavailable particle cluster flow content.
Being separately added into the 0.50g wet deposition thing collected in 3 125mL conical flasks, and add the without phosphorus culture fluid of 50mL, be subsequently added the microcystic aeruginosa of " phosphate starvation " (12d), initial concentration is 1000cell/mL.Cultivating 14d under temperature 24 ± 2 DEG C, intensity of illumination 4300lm ± 10% condition, shaking table speed is 60rpm.After 14d, measure amount of algae, calculate BAPP content by amount of algae.Result of calculation is:
BAPP=10.254 ± 1.857 μ g/L
Relative deviation ranges for 3.89%-7.90%.
Above-described embodiment, simply to illustrate that the technology of the present invention is conceived and feature, its objective is to be in that to allow one of ordinary skilled in the art will appreciate that present disclosure and to implement according to this, can not limit the scope of the invention with this.The change of every equivalence done by the essence of present invention or modification, all should be encompassed in protection scope of the present invention.

Claims (1)

1. one kind detect in shallow lake can the method for bioavailable particle cluster flow, it is characterised in that comprise the following steps:
Step 1) calculate biological effectiveness PP%: the available phosphorus content AAP and total phosphorus content Tot-P of algae in mensuration float of particle cluster flow, the percentage ratio of available for algae phosphorus content AAP and total phosphorus content Tot-P is represented particle cluster flow biological effectiveness;
Step 2) calculate the content PP of particle cluster flow: measure the total phosphorus content TP in water body and soluble phosphate content DIP, using the difference of total phosphorus content TP and soluble phosphate content DIP as particle cluster flow content PP;
Step 3) calculating can the content BAPP of bioavailable particle cluster flow: can bioavailable particle cluster flow content BAPP be biological effectiveness PP% and the product of the content PP of particle cluster flow in water body of particle cluster flow.
CN201110122990.2A 2011-05-13 2011-05-13 A kind of detect in shallow lake can the method for bioavailable particle cluster flow Expired - Fee Related CN102779222B (en)

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CN107014790B (en) * 2017-03-07 2020-02-21 闽南师范大学 Method for detecting bioavailability of copper complex by phytoplankton
CN109580514B (en) * 2019-01-09 2019-08-02 水利部交通运输部国家能源局南京水利科学研究院 A method of measuring abiotic particle cluster flow and biologic grain state phosphorus content

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