CN102776298A - Extractive of apple pomace fructose - Google Patents
Extractive of apple pomace fructose Download PDFInfo
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- CN102776298A CN102776298A CN2012102815474A CN201210281547A CN102776298A CN 102776298 A CN102776298 A CN 102776298A CN 2012102815474 A CN2012102815474 A CN 2012102815474A CN 201210281547 A CN201210281547 A CN 201210281547A CN 102776298 A CN102776298 A CN 102776298A
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Abstract
The invention discloses extractive of apple pomace fructose. The extractive is prepared by a countercurrent extraction method which comprises the following steps of (1) boiling apple pomace through water at the temperature of 85-90 DEG C for 10 minutes, smashing the apple pomace in the liquid state, and drying at the low temperature; and (2) adding ethanol water with the PH of 1.0-3.0 and the mass fraction of 65-85%, performing countercurrent extraction at the temperature of 30-60 DEG C for 4-6 hours, filtering, mixing and collecting filtrate, and concentrating to obtain the extractive of the apple pomace fructose. By means of the countercurrent extraction method, anthocyanin components can be fully extracted. Compared with the prior art, the countercurrent extraction method has the advantages of saving solvent and being high in extraction efficiency, low in cost and the like, and is suitable for industrial production.
Description
Technical field
The present invention relates to the technical field that effective ingredients in plant extracts, particularly a kind of apple residue fructose extract.
Background technology
Fructose has another name called sinistrose, is a kind of common natural sugar, the integral part of Chang Zuowei diet.It is separated from sucrose at first, and abundant content is all arranged in various fruit and the plant.For example in honey, contain 40% fructose approximately, cherry, banana, pears, apple and glucose etc. then contain 5-7%.Fructose is nutritive carbohydrate; Do not increase the digestion burden of stomach; Be delivered to liver through little intestinal absorption by arterial circulation, do not increase blood glucose value, so it is very suitable that diabetic subject, baby, gravid woman, the elderly and patient's postoperative intravenously administrable are supplemented the nutrients.Fructose is a kind of monose, is generally thick liquid, soluble in water, ethanol and ether, and the process for extracting of fructose has multiplely in the prior art, but all has problems such as technology is loaded down with trivial details, process is complicated, cost height.
Summary of the invention
Technical problem to be solved of the present invention provides a kind of apple residue fructose extract; The present invention uses counter-current to extract the fructose in the apple residue; It can extract the fructose composition more fully; Compare with art methods and to have characteristics such as the solvent of saving, extraction efficiency is high, cost is low, be suitable for suitability for industrialized production.
The present invention solves the problems of the technologies described above the technical scheme that is adopted: a kind of apple residue fructose extract, and adopt the countercurrent extraction method to prepare, said countercurrent extraction method may further comprise the steps:
Step (1): with apple residue 85-90 ℃ of poach 10min, liquid grinding, cryodrying; With
Step (2): the adding pH value is that 1.0-3.0, massfraction are the aqueous ethanolic solution of 65-85%, and 30-60 ℃ of following countercurrent extraction 4-6h merges after filtering and collect filtrating, concentrates, and gets apple residue fructose extract.
Further, in said step (2), add pH value and be 1.0, massfraction is 75% aqueous ethanolic solution, 40 ℃ of following countercurrent extraction 6 hours.
Further, the fructose content of said apple residue fructose extract is 7.92% ~ 8.17%.
" concentrating " described herein concentrates for cryogenic vacuum, and its temperature is controlled at 10 ~ 20 ℃.Spissated degree is: make the solid content of the final apple residue fructose extract of gained be controlled at 0.4% ~ 1% of its weight.
The present invention compared with prior art has following advantage:
(1) China is apple production and processing big country; The a large amount of apple residue of annual generation, utilization contain the abundant apple residue of fructose and make raw material production fructose, have important productive value; Therefore the present invention extracts fructose from apple residue, can improve the utility value that fructose output can improve apple residue again.
(2) the present invention adopts the countercurrent extraction method, can extract the anthocyanogen composition more fully, compares with art methods to have characteristics such as the solvent of saving, extraction efficiency is high, cost is low, is suitable for suitability for industrialized production.
Description of drawings
Fig. 1 is the graph of a relation of alcoholic acid mass percent (%) and leach liquor fructose content (g).
Fig. 2 is the graph of a relation of duration of contact (h) and leach liquor fructose content (g).
Fig. 3 be temperature (℃) with the graph of a relation of leach liquor fructose content (g).
Fig. 4 is the schematic representation of apparatus that a kind of apple residue fructose of the present invention method for preparing extractive uses.
Embodiment
One, the extraction process condition is investigated
1, leaching condition experiment
(1) the ethanol mass percent is to the influence of fructose compound leaching effect
In the industrial production; Owing to often contain certain moisture in the raw material; Ethanol also contains certain moisture after recycling; There is very big influence in the ethanol mass percent to the leaching of fructose compound, thus with different mass percentage ratio aqueous ethanolic solution the fructose material is carried out leaching experiment, with the fructose content trace analysis.In the ethanol mass percent is tested the influence of from raw material, leaching fructose, get 5 parts of raw materials respectively, every part of 50.00g; Experimentizing, is each 200.00ml of aqueous ethanolic solution of 1.0 with different mass percentage ratio, pH value, lixiviate 6h; Compare the amount of gained fructose, its result is as shown in Figure 1.
Can know that by Fig. 1 under the same terms, leaching ability power is followed successively by 75%>80%>70%>85%>65%, therefore, select 75% aqueous ethanolic solution as leaching agent.
(2) duration of contact is to the influence of fructose compound leaching effect
Get 6 parts of raw materials respectively, every part of 50.00g, each adds, and mass percent is 75%, pH value is that 1.0 aqueous ethanolic solution 200.00ml leaches, and is respectively 1h duration of contact, 2h, 3h, 4h, 5h, 6h, it is as shown in Figure 2 that it leaches the result.
Can know that by Fig. 2 under the room temperature (26 ℃), extraction time is 6h, leach reaction and reach balance basically; Prolong extraction time, leaching yield is constant basically.
(3) temperature is to the influence of fructose compound leaching effect
Get 6 parts of raw material powders respectively, every part of 50.00g, each adds, and the 200.00ml mass percent is 75%, pH value is 1.0 aqueous ethanolic solution; Under different temperature, leach; Extraction time is 2h, and sampling detects apple residue fructose content in the leach liquor, and the result is as shown in Figure 3.
Can know that by Fig. 3 along with the increase of extraction temperature, the leaching yield of fructose also increases accordingly; When temperature reaches 40 ℃, increase temperature, the leaching yield increase of fructose is tending towards slow.
2, four sections adverse current percolations (process diagram is seen Fig. 4)
6 ф 25.4mm * 1000mm glass have been installed have altogether been drawn together a mouthful pipe, as the leacher (leaching post) of four sections adverse current diacolation leaching experiments, wherein four operations are done week for two and are migrated.The 150.00g apple residue raw material powder of packing in every post particle (be called for short raw material, as follows), with volume pump with leaching agent (65-85% aqueous ethanolic solution), with about 5mlmin
-1Flow squeeze in first leaching post, 65-85% is with about 1cmmin
-1The speed diacolation after first post flows out the 450ml leach liquor, is connected first post and second post.The new leaching agent that volume pump is squeezed into flows out entering second post through first post; With last identical, after second post flows out 450ml black leach liquor, second post and the 3rd post are connected.The rest may be inferred, after the 4th post flows out the 450ml effluent by the time, the 4th post and the 5th post connected.Simultaneously, the volume pump outlet pipe is moved to second post upper end from first post upper end and connect (first post is withdrawn from) from system with second post.At this moment, new leaching agent advances second post through volume pump, behind three, four, five posts, flows out from the 5th post, gets the 450ml leach liquor equally; After this, new leaching agent advances the 3rd post, goes out leach liquor from the 6th post.The rest may be inferred, and constantly circulation is carried out four sections adverse current diacolation leaching experiments to reaching the leaching balance.The 9th post begins to end to the 12 post in this test; These four effusive leach liquors of post are the leach liquor during approximate equilibrium, thus when new leaching agent from advancing, through ten, 11,12 posts from the 9th post; And after the following outflow of the 12 post 450ml black leach liquor, stop leaching test.With the effusive leach liquor sampling of nine, ten, 11,12 posts, censorship, the content of mensuration fructose.(annotate: cylinder is and recycles, and nine, ten, 11,12 only represent label, do not have practical significance)
3, four sections adverse current diacolation leaching test results
Counter-current as above-mentioned makes an experiment.Every duplicate samples is 150.0g, is leach liquor with 75% aqueous ethanolic solution, 40 ℃ of following lixiviate 6h, and liquid-solid ratio is 5.Get the effusive leach liquor of nine, ten, 11,12 posts and measure, its result is as shown in table 1.
Four sections adverse current diacolations of table 1 leaching test result
Can know that from table 1 fructose content is in 6% in the apple residue, then its leaching yield of four sections adverse current diacolation lixiviation process (in leach liquor) reaches 95.0%.
Detection method:
The fructose content measuring method adopts determined by ultraviolet spectrophotometry, and concrete measuring method is referring to accounting for the content [J] that reaches fructose in the eastern determined by ultraviolet spectrophotometry fruit syrup, fine jade state college journal, 2003,10 (2): 23-27
Two, embodiment: following through specific embodiment, the present invention is made further detailed description.
Embodiment 1:
Get apple residue 500g, 85 ℃ of poach 10min, liquid grinding, cryodrying adds pH value and is 3.0, massfraction is 65% ethanolic soln 2.5L, 30 ℃ of following countercurrent extraction 4h filter the back and merge and collect filtrating, concentrate, apple residue fructose extract 105.78g.Measure the fructose content in the said apple residue fructose extract, its fructose content is 8.17%.Fructose content is in 6% in the apple residue, and then its leaching yield of four sections adverse current diacolation lixiviation process (in leach liquor) reaches 96.02%.
Said " concentrating " concentrates for cryogenic vacuum, and its temperature is controlled at 15 ℃.Spissated degree is: make the solid content of the final apple residue fructose extract of gained be controlled at 0.4% of its weight.
Embodiment 2:
Get apple residue 500g, 90 ℃ of poach 10min, liquid grinding, cryodrying adds pH value and is 1.0, massfraction is 75% ethanolic soln 2.5L, 40 ℃ of following countercurrent extraction 6h filter the back and merge and collect filtrating, concentrate, apple residue fructose extract 108.34g.Measure the fructose content in the said apple residue fructose extract, its fructose content is 7.92%.Fructose content is in 6% in the apple residue, and then its leaching yield of four sections adverse current diacolation lixiviation process (in leach liquor) reaches 95.34%.
Said " concentrating " concentrates for cryogenic vacuum, and its temperature is controlled at 10 ℃.Spissated degree is: make the solid content of the final apple residue fructose extract of gained be controlled at 0.5% of its weight.
Embodiment 3:
Get apple residue 500g, 90 ℃ of poach 10min, liquid grinding, cryodrying adds pH value and is 1.0, massfraction is 85% ethanolic soln 2.5L, 60 ℃ of following countercurrent extraction 6h filter the back and merge and collect filtrating, concentrate, apple residue fructose extract 106.11g.Measure the fructose content in the said apple residue fructose extract, its fructose content is 8.04%.Fructose content is in 6% in the apple residue, and then its leaching yield of four sections adverse current diacolation lixiviation process (in leach liquor) reaches 94.79%.
Said " concentrating " concentrates for cryogenic vacuum, and its temperature is controlled at 20 ℃.Spissated degree is: make the solid content of the final apple residue fructose extract of gained be controlled at 0.8% of its weight.
As stated, just can realize the present invention preferably.
Claims (3)
1. an apple residue fructose extract is characterized in that, adopts the countercurrent extraction method to prepare, and said countercurrent extraction method may further comprise the steps:
Step (1): with apple residue 85-90 ℃ of poach 10min, liquid grinding, cryodrying; With
Step (2): the adding pH value is that 1.0-3.0, massfraction are the aqueous ethanolic solution of 65-85%, and 30-60 ℃ of following countercurrent extraction 4-6h merges after filtering and collect filtrating, concentrates, and gets apple residue fructose extract.
2. a kind of apple residue fructose extract as claimed in claim 1 is characterized in that, in said step (2), adds pH value and be 1.0, massfraction is 75% aqueous ethanolic solution, 40 ℃ of following countercurrent extraction 6 hours.
3. a kind of apple residue fructose extract as claimed in claim 1 is characterized in that the fructose content of said apple residue fructose extract is 7.92% ~ 8.17%.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105002309A (en) * | 2015-07-08 | 2015-10-28 | 杨君 | Method for extracting fructose by using apple pomace |
| CN109266790A (en) * | 2017-07-18 | 2019-01-25 | 强宁侠 | A kind of Extractive of apple pomace fructose |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101829210A (en) * | 2010-05-21 | 2010-09-15 | 西安应化生物技术有限公司 | Method for preparing apple polyphenol |
| CN102526257A (en) * | 2010-12-14 | 2012-07-04 | 西安大地植化技术有限公司 | Industrial production method for extracting apple polyphenol from apple residues |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101829210A (en) * | 2010-05-21 | 2010-09-15 | 西安应化生物技术有限公司 | Method for preparing apple polyphenol |
| CN102526257A (en) * | 2010-12-14 | 2012-07-04 | 西安大地植化技术有限公司 | Industrial production method for extracting apple polyphenol from apple residues |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105002309A (en) * | 2015-07-08 | 2015-10-28 | 杨君 | Method for extracting fructose by using apple pomace |
| CN109266790A (en) * | 2017-07-18 | 2019-01-25 | 强宁侠 | A kind of Extractive of apple pomace fructose |
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