CN102772546A - Tibetan medicine preparation for treating essential hypertension and preparation method of Tibetan medicine preparation - Google Patents
Tibetan medicine preparation for treating essential hypertension and preparation method of Tibetan medicine preparation Download PDFInfo
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Abstract
The invention relates to a Tibetan medicine preparation for treating essential hypertension and a preparation method of the Tibetan medicine preparation. The Tibetan medicine preparation is prepared from the following raw materials in parts by weight: 100-200 parts of wood core parts of caragana jubata (pall.) Poir. or caraganachangduensis fiauf, 50-150 parts of dried overground parts of corydalis racemosa (Thunb.) pers, 50-150 parts of dried herbs of Lagotis brevituba Maxim, 50-150 parts of dried herbs of corydalis hendersonii Hemsl.(C.nepaiesis kitamura) and C.mucionifera Maxim., 50-150 parts of paste of dried wood of rhamnus parvifolia Bunge or 500-1,500 parts of dried wood of rhamnus parvifolia Bunge, and 80-180 parts of dried mature fruits of phyllanthus emblica L. The Tibetan medicine preparation provided by the invention has the effects of treating the essential hypertension and reducing blood pressure, and can be used for preparing a medicine for reducing blood pressure.
Description
Technical field
The present invention relates to the field of Chinese medicines, be specifically related to a kind of Tibetan medicinal preparation and preparation method thereof with hypotensive effect.
Background technology
Hypertension is meant that the cause of disease is not clear and definite as yet, is higher than a kind of independent disease that be main clinical manifestation normal range with systemic arterial blood pressure.Hypertension be a kind of be characteristic with the arterial pressure rising, can be functional or the systemic disease of organic change with heart, blood vessel, brain and kidney and other organs, it has the branch of essential hypertension and secondary hypertension.Essential hypertension is called hypertension again, is to be main clinical manifestation and the independence disease of etiology undetermined accounts for more than 90% of hypertensive patient with the hypertension.Secondary hypertension is called symptomatic hypertension again, and is clear and definite in the hypertensive cause of disease of this apoplexy due to endogenous wind, is one of performance of certain disease.The reason of hypertension incidence is a lot, can be divided into two aspects of h and E.Chinese medicine thinks that human five internal organs's six internal organs all have respective projection on foot, and foot is the starting point of Three Yin Channels of Foot, is again the terminating point of the three yang channels of foots, and more than 60 acupuncture point just arranged below the ankle joint.
The early symptom of hypertension is dizziness, headache, cardiopalmus, insomnia, anxiety, agitation, tired etc.Can involve the heart, brain, kidney organ gradually later on, can concurrent hypertensive heart disease when serious, pathological changes such as renal failure, cerebrovascular accident.
The cause of disease and the pathogenesis of hypertension are described below, wherein the cause of disease: the 1) age: sickness rate has the trend that increases with age growth, and person's sickness rate is high more than 40 years old.2) Sal: take in the many persons of Sal, the hypertension incidence rate is high, and the Sal of thinking＜2g/ day is arranged, and hypertension takes place hardly; 3-4g/ day, hypertension incidence rate 3%, 4-15g/ day, sickness rate 33.15%,＞20g/ day sickness rate 30%.3) body weight: overweight people's sickness rate is high.4) heredity: approximately the half hyperpietic has family history.5) environment and occupation: the working environment of noise is arranged, and overwrought mental work all is prone to hypertension takes place, and the high pressure sickness rate in the city is higher than the rural area.
Pathogenesis: 1) talk about from modal overweight people's hypertension; Too fat hyperliposis causes certain extruding to blood vessel, after pipeline is extruded; Power source need strengthen power just possibly make original circulation reach circulation; Power source power strengthens, and pipeline pressure also can strengthen thereupon, has just formed high pressure.2) thrombosis that causes of inner blood and other diseases causes, and the metabolism of blood is discharged thoroughly inadequately, forms dirt at pipe interior, and pipeline is caused certain obstruction, and pressure is raise.3) sclerosis of senile pipeline and the sclerosis of disease property, the pipeline hardened words that give a discount can cause high pressure.4) disease property blood capillary stops up and the obstruction of traumatic blood capillary, also is one of factor wherein.5) body disease property causes, a part of hyperglycemic patients is because digestive system is too excited, aspect the intestines and stomach, pathological changes is arranged, and forming certain blood circulation obstruction aspect the intestines and stomach body, also can cause high pressure.6) the congenital and posteriori disappearance of heart aspect.7) cerebrovascular disease causes.8) the dry high pressure that causes of blood.
Depressor is mainly brought into play pressure reduction effect through influencing sympathetic nervous system, renin angiotensin aldosterone system and endothelin system etc. to the system that the physiological regulation of blood pressure plays an important role.Depressor commonly used has: sympathetic nerve depressant such as clonidine, guanidine second are fixed, prazosin, Propranolol etc.; Main depressor such as the hydrochlorothiazide that influences blood volume; Tonin inhibitor such as captopril; Angiotensin receptor antagonist (ARB) is like Candesartan; Calcium antagonists such as nifedipine and direct vasodilator smooth muscle medicine such as hydralazine etc.
Hypertensive Drug therapy generally is to take Western medicine and two kinds of Therapeutic Method of Chinese medicine.The principle of Western medicine blood pressure lowering generally is through blood vessel dilating by force; Means such as increase cardiac contractile force reach the effect of blood pressure lowering; But Western medicine depressor shortcoming is to have side effect; And side effect meeting major injury people intravital internal organs, especially liver, kidney, so the hypertensive patient can not rely on Western medicine fully.Though treatment by Chinese herbs hypertension drug effect is slow, the course of treatment is longer, is treating both the principal and secondary aspects of a disease.Hypertension worldwide still belongs to a difficult problem, also can't effect a radical cure at present, thereby the hyperpietic need so should select gentleness, slow, lasting, the little Chinese medicine of side effect, just can not bring infringement to health through taking medicine controlling blood pressure for a long time.
Tibetan medicine Six-element caragana soup looses and records in " the Sanitation Ministry medicine standard " Tibetan medicine fascicle, and standard is numbered WS3-BC-0292-95, is made up of Cortes seu Caulis Caraganae jubatae, Radix Inulae, Fructus Tsaoko, Fructus Amomi Rotundus, Semen Arecae, Rhizoma Alpiniae Officinarum Six-element medicine.Be mainly used in blending blood, dragon, cough, rapid breathing, kidney pain in the lumbar region pain, seminal emission, pudendum fistula that impairment caused by overstrain causes, " lower limb vasculitis " is sick, ascites.Wherein each primary raw material medicine is following:
Cortes seu Caulis Caraganae jubatae: record " the Sanitation Ministry medicine standard " (first of Tibetan medicine), these article are the woody part heartwood of leguminous plant Cortes seu Caulis Caraganae jubatae Caragana jubata (pal1.) Poir. and Changdu caragana Caraganachangduensis fiauf.Cut and get the maroon woody part, cut off, dry in the shade.
Radix Inulae: record in " 2010 editions one one of Chinese pharmacopoeia, 27 pages of appendix are the dry root of the total shape Radix Aristolochiae of feverfew Inula racemosa Hook.f..Uproot with autumn end at the beginning of spring, remove clean residual stem, earth, section is dried.
Fructus Tsaoko: record that " one one of Chinese pharmacopoeia version in 2010, the 222nd page, these article are the dry mature fruit of zingiberaceous plant Fructus Tsaoko Amomum tsao-ko Crevost et Lemaire.Gather during fruit maturation autumn, removes impurity, dries or cold drying.
Fructus Amomi Rotundus: record that " one one of Chinese pharmacopoeia version in 2010, the 156th page, these article are the dry mature fruit of zingiberaceous plant Fructus Amomi Rotundus Amomum Kravanh Pierre ex Gagnep. or amomum compactum Soland ex Maton Amomum compactum Soland ex Maton.Be divided into " former Fructus Amomi Rotundus " and " the white bandit of Indonesia " by place of production difference.
Semen Arecae: record that " one one of Chinese pharmacopoeia version in 2010, the 342nd page, these article are the dry mature seed of babassu Semen Arecae Areca catechu L..The mature fruit of gathering at the beginning of spring end to autumn, behind decocting in water, drying is removed peel, takes out seed, drying.
Rhizoma Alpiniae Officinarum: record that " one one of Chinese pharmacopoeia version in 2010, the 270th page, these article are the dry rhizome of zingiberaceous plant Rhizoma Alpiniae Officinarum Alpinia of ficinarum Hance.Excavate at the beginning of autumn late summer, remove fibrous root and residual scale, clean, cut off, dry.
Also do not have at present relevant Tibetan medicine Six-element caragana soup to loose and be used to prepare the report of treating the hypotensive effect medicine.
Summary of the invention
One object of the present invention be to provide a kind of Tibetan medicinal preparation of treating essential hypertension, its preparation method with and purposes in the preparation antihypertensive drugs.
The present invention improves requirement according to the Tibetan medicine quality standard; The suggestion that combines Tibet Autonomous Region food and medicine check relevant expert of institute that Six-element caragana soup in the existing ministry standard is loose and writes out a prescription and revise simultaneously; According to " Tibetan medicine standard error correction inventory preparation part (Tibet) " the 39th page part medical material and dosage in this prescription are adjusted; Developed new recipe, promptly formed by cream, Fructus Phyllanthi Six-element medicines such as ZANGJINJIER, BAXIAGA, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.), Herba Corydalis, Song Sheng, wherein:
ZANGJINJIER: record " the Sanitation Ministry medicine standard " (first of Tibetan medicine), these article are the woody part heartwood of leguminous plant Cortes seu Caulis Caraganae jubatae Caragana jubata (pall.) Poir. and Changdu caragana Caraganachangduensis fiauf.Cut and get the maroon woody part, cut off, dry in the shade.
BAXIAGA: record in " Tibet Autonomous Region local quality of medicinal material standard ", standard No. is XZ-BC-0001-2003, is the dry aerial parts of the little brightly yellowish violet Corydalis racemosa of bloodroot (Thunb.) pers, dry or cut off dry subsequent use.
Lagotis clarkei Hook. F (Lagotis brevituba Maxim.): record that " one one of Chinese pharmacopoeia version in 2010, the 249th page, these article are the dry herb of the short tube of goatweed Radix Pecteilis susannae Lagotis brevituba Maxim.Summer, autumn gathered when two seasons, the flowers are in blossom, removed impurity, cleaned, and dried in the shade.
Herba Corydalis: record in " the Sanitation Ministry medicine standard " (first of Tibetan medicine); Standard numbering: WS3-BC-0114-95; These article are the dry herb of bloodroot Herba Corydalis Corydalis hendersonii Hemsl. (C.nepaiesis kitamura) and flat handle Herba corydalis racemosae C.mucionifera Maxim.; Grubbed out summer, cleans, and dries in the shade.
Song Sheng etc.: these article are the desiccated wood of the Fructus Rhamni parvifoliae Rhamnus parvifolia Bunge of Rhamnaceae plant Rhamnella gilgitica Rhamnella gilgitica Mansf.et Melch..All can gather the whole year, removes and debark, and the section of being sawn into is dried after riving.
Fructus Phyllanthi: record in " one one of Chinese pharmacopoeia version in 2010, the 167th page, these article are the dry mature fruit of euphorbia plant Fructus Phyllanthi Phyllanthus emblica L..Winter to time spring gathers during fruit maturation, removes impurity, drying.
For realizing above-mentioned purpose, technical scheme of the present invention is following:
A kind of Tibetan medicinal preparation of treating essential hypertension is processed by following crude drug: 30-200 weight portion, Fructus Phyllanthi 80-180 weight portion such as cream 50-150 weight portion such as ZANGJINJIER 100-200 weight portion, BAXIAGA 50-150 weight portion, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.) 50-150 weight portion, Herba Corydalis 50-150 weight portion, Song Sheng or Song Sheng.
Further, above-mentioned preparation is processed by following crude drug: 50-150 weight portion, Fructus Phyllanthi 100-150 weight portion such as cream 80-120 weight portion such as ZANGJINJIER 120-180 weight portion, BAXIAGA 80-120 weight portion, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.) 80-120 weight portion, Herba Corydalis 80-120 weight portion, Song Sheng or Song Sheng.
Further, above-mentioned Tibetan medicinal preparation is processed by following crude drug: 100 weight portions, Fructus Phyllanthi 130 weight portions such as cream 100 weight portions such as ZANGJINJIER 150 weight portions, BAXIAGA 100 weight portions, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.) 100 weight portions, Herba Corydalis 100 weight portions, Song Sheng or Song Sheng.
Above-mentioned Tibetan medicinal preparation can be prepared into pharmaceutically acceptable preparation, like pill, tablet, capsule, oral liquid, syrup, granule, powder, drop pill, slow releasing preparation or controlled release preparation.
Through experiment showed, that Tibetan medicinal preparation of the present invention has the effect of treatment essential hypertension and blood pressure lowering, so another object of the present invention provides above-mentioned Tibetan medicinal preparation in the application for preparing on the antihypertensive drugs.
The present invention further provides the method for preparing of above-mentioned Tibetan medicinal preparation, comprising:
With cream such as ZANGJINJIER, BAXIAGA, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.), Herba Corydalis, Song Sheng or Song Sheng etc., the remove impurity respectively of Fructus Phyllanthi crude drug, cleaning, drying, be ground into coarse powder, sieve, mixing promptly gets.
The another kind of method for preparing of Tibetan medicinal preparation of the present invention; Comprise cream such as ZANGJINJIER, BAXIAGA, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.), Herba Corydalis, Song Sheng or Song Sheng etc., Fructus Phyllanthi crude drug are carried out remove impurity respectively, clean, mix; With the 50-90% alcohol reflux of crude drug 5-15 times of weight 1-3 time, extracted 0.5-2.5 hour at every turn, collect and merge extractive liquid; Filter, extracting solution is condensed into the concentrated solution that relative density is 1.0-1.5; Or with extracting solution feed absorption, washing, the flow velocity of resolving is 0.1-10 times of bed volume/hour D101 or D101B or XDA-1 or XDA-1B or HPD-300 macroporous adsorbent resin or cation exchange resin; Use earlier water washing; Again with the 10-30% washing with alcohol; Resolve with 50-70% ethanol at last, collect ethanol liquid, reclaim ethanol and it is condensed into the concentrated solution that relative density is 1.0-1.5; Add the adjuvant of pharmaceutically accepting, process said Tibetan medicinal preparation.
The another kind of method for preparing of Tibetan medicinal preparation of the present invention; Comprise cream such as ZANGJINJIER, BAXIAGA, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.), Herba Corydalis, Song Sheng or Song Sheng etc., the remove impurity respectively of Fructus Phyllanthi medical material, clean, mix, stir infiltration 0.5-2 hour with the 50-90% ethanol of crude drug 0.5-1.5 times of weight, in the percolation jar of packing into; With the 50-90% ethanol percolate extraction of crude drug 5-15 times of weight 2-4 time; Collect and merge extractive liquid,, filter, extracting solution is condensed into the concentrated solution that relative density is 1.0-1.5; Or with extracting solution feed absorption, washing, the flow velocity of resolving is 0.1-10 times of bed volume/hour D101 or D101B or XDA-1 or XDA-1B or HPD-300 macroporous adsorbent resin or cation exchange resin; Use earlier water washing; Again with the 10-30% washing with alcohol; Resolve with 50-70% ethanol at last, collect ethanol liquid, reclaim ethanol and it is condensed into the concentrated solution that relative density is 1.0-1.5; Add the adjuvant of pharmaceutically accepting, process said Tibetan medicinal preparation.
The another kind of method for preparing of Tibetan medicinal preparation of the present invention; Comprise cream such as ZANGJINJIER, BAXIAGA, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.), Herba Corydalis, Song Sheng or Song Sheng etc., the remove impurity respectively of Fructus Phyllanthi medical material, clean, mix; 50-90% alcohol dipping with crude drug 5-15 times of weight is extracted 2-4 time, floods 8-16 hour at every turn, collects and merge extractive liquid; Filter, extracting solution is condensed into the concentrated solution that relative density is 1.0-1.5; Or with extracting solution feed absorption, washing, the flow velocity of resolving is 0.1-10 times of bed volume/hour D101 or D101B or XDA-1 or XDA-1B or HPD-300 macroporous adsorbent resin or cation exchange resin; Use earlier water washing; Again with the 10-30% washing with alcohol; Resolve with 50-70% ethanol at last, collect ethanol liquid, reclaim ethanol and it is condensed into the concentrated solution that relative density is 1.0-1.5; Add the adjuvant of pharmaceutically accepting, process said Tibetan medicinal preparation.
Another method for preparing of Tibetan medicinal preparation of the present invention comprises cream such as ZANGJINJIER, BAXIAGA, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.), Herba Corydalis, Song Sheng or Song Sheng etc., Fructus Phyllanthi is carried out remove impurity respectively, cleans, mixes, and boils 2-4 time with the decocting of crude drug 5-15 times of weight; The each decoction 0.5-2.5 hour; Collect and merge extractive liquid,, filter, extracting solution is condensed into the concentrated solution that relative density is 1.0-1.5; Add the adjuvant of pharmaceutically accepting, process said Tibetan medicinal preparation.
The present invention adopts Tibetan medicine prescribed treatment essential hypertension; Because Tibetan medicine generally is grown on the snowy peak of more than 3800 meter of height above sea level; So medicine does not have contaminated, the active ingredient of medicine does not receive the interference and the destruction of other material, so its drug effect is also wanted instant effect than Chinese medicine.The maximum characteristics that Tibetan medicine is cured the disease are directly to go into focus, directly suppress virus, fundamentally go treatment from body.In addition, the collection of Tibetan medicine is also different with Chinese medicine, and Chinese medicine is and then to go season to gather, and Tibetan medicine is when the medical material effective component content is the highest, to gather.So when essential hypertension was treated, if can adopt the medicine of Tibetan medicine crude drug as the treatment essential hypertension, the injury to human body will reduce much relatively so.
The specific embodiment
Medicine source: medicine 1 of the present invention (being prepared from) according to embodiment in the description 2; Medicine 2 of the present invention (being prepared from) according to embodiment in the description 3, medicine 3 of the present invention (being prepared from), medicine 4 of the present invention (being prepared from) according to embodiment in the description 6 according to embodiment in the description 4; Medicine 5 of the present invention (being prepared from) according to embodiment in the description 7; Medicine 6 of the present invention (being prepared from) according to embodiment in the description 8, medicine 7 of the present invention (being prepared from), medicine 8 of the present invention (being prepared from) according to embodiment in the description 11 according to embodiment in the description 10; Medicine 9 of the present invention (being prepared from) according to embodiment in the description 12; Medicine 10 of the present invention (being prepared from) according to embodiment in the description 14, medicine 11 of the present invention (being prepared from), medicine 12 of the present invention (being prepared from) according to embodiment in the description 16 according to embodiment in the description 15; Pitchy is Powdered, is provided by XiZang QiZheng Tibetan pharmaceuticals Co., Ltd.With 0.5%CMC (hydroxy methocel) medicine 1-12 of the present invention all is made into the medicinal liquid of 0.54g crude drug/10ml before the test, presses 0.54g crude drug/kg administration, confession rat oral gavage administrable, the administration volume is 1ml/100g.
Animal origin: select satisfactory original hypertensive rat (SHR) for use, male 13 ages in week, body weight 220～250g.12 of this especially big Mus (WKY) of Tokyo prestige, 13 ages in week, as normal control, available from Beijing Vital River Experimental Animals Technology Co., Ltd., the animal quality certification number: SCXK (capital) 2006-0009.Rat feeding is indoor in constant temperature (22 ± 2 ℃), and standard feed is freely drunk water.
Positive control medicine source: captopril: commercially available, specification 25mg/ sheet, Tianjin Jinshi Pharmaceutical Co., Ltd. produces, and lot number 20100501 is mixed with the 1.5mg/mL medicinal liquid with 0.5%CMC, supplies rat oral gavage usefulness, and the administration volume is 1ml/100g.
The experiment reagent source: cholesterol (TC) test kit, Zhongsheng Beikong Biological Science & Technology Co., Ltd. provides, lot number 100441.201004; Triglyceride (TG) test kit, Zhongsheng Beikong Biological Science & Technology Co., Ltd. provides, lot number 100441.201005; Low density lipoprotein, LDL (LDL) test kit, Zhongsheng Beikong Biological Science & Technology Co., Ltd. provides, lot number 100231.201004; High density lipoprotein (HDL) test kit, Zhongsheng Beikong Biological Science & Technology Co., Ltd. provides, lot number 100201.201005; Fibrinogen (Fbg) is measured test kit, and the North Sea, Shanghai biotechnology Engineering Co., Ltd provides, lot number BH-25110.
Insulinase linked immunosorbent assay test kit (Mercodia Rat Insulin ELISA), MercodiaAb, Sylveniusgatan8A, SE-75450Uppsala, Sweden provides, lot number 10-1124-01; Glucose (GLu) test kit, Zhongsheng Beikong Biological Science & Technology Co., Ltd. provides, lot number 100401.201004.
Superoxide dismutase (SOD) test kit, Nanjing is built up bio-engineering research and is provided; Lot number 20100403,100 parts of specifications; Nitric oxide (NO) test kit, Nanjing is built up bio-engineering research and is provided, lot number 20100613,100 parts of specifications; Malonaldehyde (MDA) test kit, Nanjing is built up bio-engineering research and is provided, lot number 20100613,100 parts of specifications.
Plasma angiotensinogen II (AngII) radioimmunological kit, Mei Dike Bioisystech Co., Ltd in Beijing provides, lot number HY-053; PA (ALD) radioimmunological kit, Mei Dike Bioisystech Co., Ltd in Beijing provides, lot number HY-054; Endothelin (ET) radioimmunological kit, Mei Dike Bioisystech Co., Ltd in Beijing provides, lot number HY-040; RA (PRL1) radioimmunological kit, Mei Dike Bioisystech Co., Ltd in Beijing provides, lot number HY-134.
Pathology reagent: formalin, ethanol, xylene, paraffin, dyestuff etc.
Main experimental apparatus source: BP-98A rat blood pressure analyzer, the soft grand Bioisystech Co., Ltd of Japan.
7020 type automatic clinical chemistry analyzers, HIT.
The LDZ5-2 low speed autobalancing centrifuge, centrifuge company is found in capital, Beijing.
JA1003 type electronic balance, Shanghai Hengping Science Instrument Co., Ltd..
37 ℃ of waters bath with thermostatic control, Shanghai Shiping Experiment Equipment Co., Ltd..
STAT FAX 2100 full-automatic ELIASAs, Awareness Technology Inc.USA.
95 editions 300 type semi-automatic biochemical analyzers, Dutch Rittal GmbH.
γ-911 is automatically put and is exempted to count instrument, Chinese University of Science and Technology's industry head office.
The GB/T14899-94 digimatic calipers, Guanglu Digital Measure-Control Co., Ltd., Guilin.
BMJ-1 biological tissue embedding machine, Tianjin Aviation Mechano-Electrical Co..
The LEICARM2135 microtome, German LEICARM.
Olympus microscope and DP71 photograph analytical system, Japan.
1 experimental technique
1.1SHR arteria caudalis blood pressure measuring method.
Adopt rat arteria caudalis pulse manometry.Before the pressure measurement, rat is put into 37 ± 1 ℃ heat-preservation cylinder, pressure measurement on the pressure measurement platform is fixed in about 10 minutes of preheating then.Under the rat rest state, continuous blood pressure measuring 5 times is averaged as the pressure measurement result.In the pressure measurement process, it is soft that action is wanted, and avoids causing that rat is uneasy.Before the formal experiment, 2 weeks of adaptability pressure measurement.Treat that rat conforms, behind the blood pressure stabilization, beginning is experiment formally.
Single-dose measuring blood pressure method: the Fundamentals of Measurement blood pressure is a blood pressure before the administration, measures the blood pressure of 1h, 3h, 6h, 12h after the disposable administration.Every rat is measured 5 blood pressures, averages.
Successive administration measuring blood pressure method: measure blood pressure weekly 1 time, every rat is measured 5 blood pressures, averages, continuously 4 weeks of pressure measurement.
The index of measuring comprise systolic pressure (Systolic Blood Pressure, SBP), diastolic pressure (Diastolic Blood Pressure, DBP), mean arterial pressure (Mean Arterial Blood Pressure, MBP) and heart rate (Heart Reat, HR).
1.2 divide into groups and administration
1.2.1 divide into groups
Measure the basic blood pressure of every SHR before the administration, press basic blood pressure and divide into groups, be divided into 15 groups, 12 every group or 13, comprise model group; 1 group of medicine of the present invention, 2 groups of medicines of the present invention, 3 groups of medicines of the present invention, 4 groups of medicines of the present invention, 5 groups of medicines of the present invention; 6 groups of medicines of the present invention, 7 groups of medicines of the present invention, 8 groups of medicines of the present invention, 9 groups of medicines of the present invention, 10 groups of medicines of the present invention; 11 groups of medicines of the present invention, 12 groups of medicines of the present invention, the captopril positive controls, other establishes normal control group (WKY).
1.2.2 medication
WKY normal arterial pressure matched group and model group are irritated stomach 0.5%CMC, and the administration volume is the 1mL/100g body weight.
Respectively tried drug group: medicine 1-12 group of the present invention is 0.54g/kg/d; Positive drug captopril matched group 15mg/kg/d, gastric infusion, administration volume are the 1mL/100g body weight.
The single-dose method: administration at once is 1 time behind the preceding blood pressure of measurement administration, and dosage is the same.
The successive administration method: every day gastric infusion once, continuous 4 weeks.Survey body weight weekly one time, according to body weight adjustment dosage.
1.3 sample collection and detection method
1.3.1 sample collection
Blood preparation: pharmaceutical intervention is used chloral hydrate anesthesia after 4 weeks, abdominal aortic blood 8ml, and 37 ℃ of water-bath 30min with the centrifugal 10min separation of serum of 3500r/min, and are sub-packed in the 1.5ml Ep pipe.(2～8 ℃ deposit or multigelation is avoided in-20 ℃ of freezing preservations).
Sick inspection BIAO and BEN: dissect rat, get brain, heart, kidney, and it is fixing to put into 10% formalin solution, routine paraffin wax embedding, section, HE dyeing, om observation.
It is dirty to core, and respectively weighing left ventricle, chamber weight whole-heartedly are with kind of calliper cardiac septum thickness, left ventricular posterior wall thickness
1.3.2 detection method
Use Hitachi's 7020 automatic clinical chemistry analyzers, adopt two point method to detect triglyceride (TG) concentration, cholesterol (TC) concentration, glucose (GLu) concentration.Detect HDL-C (HDL) concentration and low-density lipoprotein cholesterol (LDL) concentration with the removing method.
Radioimmunology detects PA (ALD), plasma angiotensinogen II (AngII), Endothelin (ET), feritin (PRL1) activity.
Adopt double antibodies sandwich method enzyme-linked immunosorbent assay (ELISA) to detect the rat Langerhans islet cellulose content.
Superoxide dismutase (SOD) adopts xanthine oxidase; Malonaldehyde (MDA) adopts thiobarbituricacid (TBA) colorimetry; Nitric oxide (NO) adopts nitrate reductase method.
The applying electronic digital display calliper is measured cardiac septum thickness, left ventricular posterior wall thickness.Applying electronic balance weighing myocardium of left ventricle weight, chamber cardiac muscle weight whole-heartedly.
1.4 statistical method
Adopt the SPSS11.0 software kit to carry out statistical procedures; Data are expression with
, relatively adopts one factor analysis of variance between group.
2 results
2.1 the variation of experimental session rat body weight
Each is organized rat body weight and increases in time, and model group and WKY group rat body weight have significant difference (P＜0.01).The result sees table 1.
Table 1 experimental session rat body weight (g) changes
Annotate: compare with the WKY group
##P＜0.01.
[experimental example 1] medicine single-dose of the present invention is to the influence of SHR arteria caudalis blood pressure and heart rate
1. single-dose is to the influence of SHR arteria caudalis systolic pressure (SBP)
The model group systolic pressure is significantly higher than WKY group (P＜0.01); Single is irritated clothes medicine of the present invention, and medicine 1-12 group of the present invention relatively has significant difference (P＜0.05～0.01) in the effect that administration 3h, 6h, 12h all have obvious reduction arteria caudalis systolic pressure with model group; Captopril group 1h, 6h, 12h after administration can obviously reduce the arteria caudalis systolic pressure, with model group significant difference (P＜0.05～0.01) are arranged relatively.The result sees table 2.
Table 2 medicine single-dose of the present invention is to the influence of SHR arteria caudalis systolic pressure (mmHg)
Annotate: 1. compare with the WKY group
##P＜0.01; Compare with model group
*P＜0.05,
*P＜0.01.
2. numerical value is blood pressure before this time point blood pressure * 100/ administration in the bracket.
2. single-dose is to the influence of SHR arteria caudalis diastolic pressure (DBP)
The model group diastolic pressure is significantly higher than WKY group (P＜0.01); Single is irritated clothes medicine of the present invention, and medicine 1-12 group of the present invention relatively has significant difference (P＜0.05～0.01) in the effect that administration 6h, 12h all have obvious reduction auterial diastole to press with model group; Captopril group can obviously reduce auterial diastole at administration 1h, 6h, 12h presses, and with model group significant difference (P＜0.05～0.01) is arranged relatively.The result sees table 3.
Table 3 medicine single-dose of the present invention is to the influence of SHR arteria caudalis diastolic pressure (mmHg)
Annotate: 1. compare with the WKY group
##P＜0.01; Compare with model group
*P＜0.05,
*P＜0.01.
2. numerical value is blood pressure before this time point blood pressure * 100/ administration in the bracket.
3. single-dose is to the influence of SHR arteria caudalis mean arterial pressure (MBP)
The model group mean arterial pressure is significantly higher than WKY group (P＜0.01); Single is irritated clothes medicine of the present invention, and medicine 1-12 group of the present invention relatively has significant difference (P＜0.05～0.01) in the effect that administration 3h, 6h, 12h all have obvious reduction mean arterial pressure with model group; Captopril group 1h, 6h, 12h after administration can obviously reduce mean arterial pressure, with model group significant difference (P＜0.05～0.01) are arranged relatively.The result sees table 4.
Table 4 medicine single-dose of the present invention is to the influence of SHR arteria caudalis mean arterial pressure (mmHg)
Annotate: 1. compare with the WKY group
##P＜0.01; Compare with model group
*P＜00.5,
*P＜00.1.
2. numerical value is blood pressure before this time point blood pressure * 100/ administration in the bracket.
4. single-dose is to the influence of SHR heart rate (HR)
Single is irritated clothes medicine of the present invention does not have influence basically to the SHR heart rate.The result sees table 5.
Table 5 medicine single-dose of the present invention is to the influence of SHR heart rate (inferior/minute)
Annotate: 1. compare with the WKY group
#P＜0.05; Compare with model group
*P＜0.05.
2. numerical value is heart rate before this time point heart rate * 100/ administration in the bracket.
Conclusion: single is irritated clothes medicine of the present invention, and medicine 1-12 group of the present invention does not have influence in the effect that administration 3h, 6h, 12h all can obviously reduce SHR diastolic pressure and mean arterial pressure to the SHR heart rate basically.
[experimental example 2] 4 weeks of medicine successive administration of the present invention are to the influence of SHR arteria caudalis blood pressure and heart rate
1. 4 weeks of successive administration are to the influence of SHR arteria caudalis systolic pressure (SBP)
The model group systolic pressure is significantly higher than WKY group (P＜0.01); Continuous irrigation clothes medicine of the present invention, medicine 1-12 group of the present invention relatively has significant difference (P＜0.05～0.01) in the effect that obvious reduction arteria caudalis systolic pressure is all arranged in the 1st week of administration with model group; Captopril group is in the beginning blood pressure lowering of administration the 1st week, and hypotensive effect continues to 4 weeks after the administration, with model group comparing difference significantly (P＜0.01).The result sees table 6.
Table 6 medicine successive administration of the present invention is to the influence of SHR arteria caudalis systolic pressure (mmHg)
Annotate: 1. compare with the WKY group
##P＜0.01; Compare with model group
*P＜0.05,
*P＜0.01.
2. numerical value is blood pressure before this time point blood pressure * 100/ administration in the bracket.
2. 4 weeks of successive administration are to the influence of SHR arteria caudalis diastolic pressure (DBP)
The model group diastolic pressure is significantly higher than WKY group (P＜0.01); Continuous irrigation clothes medicine of the present invention, medicine 1-12 group of the present invention relatively has significant difference (P＜0.05～0.01) in the effect that obvious reduction arteria caudalis diastolic pressure is all arranged in the 1st week of administration with model group; Captopril group is in the beginning blood pressure lowering of administration the 1st week, and hypotensive effect continues to 4 weeks after the administration, with model group comparing difference significantly (P＜0.01).The result sees table 7.
Table 7 medicine successive administration of the present invention is to the influence of SHR arteria caudalis diastolic pressure (mmHg)
Annotate: 1. compare with the WKY group
##P＜0.01; Compare with model group
*P＜0.05,
*P＜0.01.
2. numerical value is blood pressure before this time point blood pressure * 100/ administration in the bracket.
3. 4 weeks of successive administration are to the influence of SHR arteria caudalis mean arterial pressure (MBP)
The model group mean arterial pressure is significantly higher than WKY group (P＜0.01); Continuous irrigation clothes medicine of the present invention, medicine 1-12 group of the present invention relatively has significant difference (P＜0.05～0.01) in the effect that obvious reduction mean arterial pressure is all arranged in the 1st week of administration with model group; Captopril group begins blood pressure lowering after 1 week of administration, hypotensive effect continues to 4 weeks after the administration, with model group comparing difference significantly (P＜0.01).The result sees table 8.
Table 8 medicine successive administration of the present invention is to the influence of SHR arteria caudalis mean arterial pressure (mmHg)
Annotate: 1. compare with the WKY group
##P＜0.01; Compare with model group
*P＜0.05,
*P＜0.01.
2. numerical value is blood pressure before this time point blood pressure * 100/ administration in the bracket.
4. 4 weeks of successive administration are to the influence of SHR heart rate (HR)
The continuous irrigation clothes do not have influence to the SHR heart rate in 4 weeks of medicine of the present invention basically.The result sees table 9.
Table 9 medicine successive administration of the present invention is to the influence of SHR heart rate (inferior/minute)
Annotate: 1. compare with the WKY group
##P＜0.01; Compare with model group
*P＜0.05,
*P＜0.01.
2. numerical value is blood pressure before this time point blood pressure * 100/ administration in the bracket.
Conclusion: continuous irrigation clothes medicine of the present invention, medicine 1-12 group of the present invention does not have influence in the effect that obvious reduction systolic pressure, diastolic pressure and mean arterial pressure are all arranged in the 1st week of administration to the SHR heart rate basically.
Following embodiment all can realize the effect of above-mentioned experimental example.
The preparation of [embodiment 1] Tibetan medicine slow releasing preparation of the present invention
Cream 50g, Fructus Phyllanthi 180g such as ZANGJINJIER 100g, BAXIAGA 150g, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.) 50g, Herba Corydalis 150g, Song Sheng;
Cream, Fructus Phyllanthi medical materials such as ZANGJINJIER, BAXIAGA, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.), Herba Corydalis, Song Sheng are carried out remove impurity respectively, clean, mix; With 65% alcohol reflux of crude drug 10 times of weight 3 times; The each extraction 2 hours; Collect and merge extractive liquid,, filter, it is 1.15 concentrated solution that extracting solution is condensed into relative density; Add conventional adjuvant,, process Tibetan medicine slow releasing preparation of the present invention according to common process.
The preparation of [embodiment 2] Tibetan medicine pill of the present invention
Cream 60g, Fructus Phyllanthi 90g such as ZANGJINJIER 110g, BAXIAGA 60g, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.) 60g, Herba Corydalis 60g, Song Sheng;
Cream, Fructus Phyllanthi medical materials such as ZANGJINJIER, BAXIAGA, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.), Herba Corydalis, Song Sheng are carried out remove impurity, cleaning, drying respectively, be ground into coarse powder, sieve, mixing adds conventional adjuvant, according to common process, processes Tibetan medicine pill of the present invention.
The discriminating of [embodiment 3] Tibetan medicine oral liquid of the present invention
Cream 60g, Fructus Phyllanthi 90g such as ZANGJINJIER 110g, BAXIAGA 60g, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.) 60g, Herba Corydalis 60g, Song Sheng;
Cream, Fructus Phyllanthi medical materials such as ZANGJINJIER, BAXIAGA, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.), Herba Corydalis, Song Sheng are carried out remove impurity respectively, clean, mix, soaked into 1.5 hours with the 70% ethanol stirring of crude drug 1.5 times of weight, in the percolation jar of packing into; Use 70% ethanol percolate extraction 3 times of crude drug 12,10,8 times of weight respectively, collect and merge extractive liquid,, filter; With extracting solution feed absorption, washing, the flow velocity of resolving is 0.1-10 times of bed volume/hour the D101 macroporous adsorbent resin; Earlier use water washing,, resolve with 50% ethanol at last again with 10% washing with alcohol; Collect ethanol liquid, reclaiming ethanol and it is condensed into relative density is 1.35 concentrated solution; Add conventional adjuvant,, process Tibetan medicine oral liquid of the present invention according to common process.
The discriminating of [embodiment 4] Tibetan medicine soft capsule of the present invention
Cream 60g, Fructus Phyllanthi 90g such as ZANGJINJIER 110g, BAXIAGA 60g, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.) 60g, Herba Corydalis 60g, Song Sheng;
Cream, Fructus Phyllanthi medical materials such as ZANGJINJIER, BAXIAGA, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.), Herba Corydalis, Song Sheng are carried out remove impurity respectively, clean, mix, boil 3 times, decocted 2 hours at every turn with the decocting of crude drug 10 times of weight; Collect and merge extractive liquid; Filter, it is 1.15 concentrated solution that extracting solution is condensed into relative density, adds conventional adjuvant; According to common process, process Tibetan medicine soft capsule of the present invention.
The preparation of [embodiment 5] Tibetan medicine syrup of the present invention
Cream 80g, Fructus Phyllanthi 100g such as ZANGJINJIER 120g, BAXIAGA 80g, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.) 80g, Herba Corydalis 80g, Song Sheng;
Cream, Fructus Phyllanthi medical materials such as ZANGJINJIER, BAXIAGA, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.), Herba Corydalis, Song Sheng are carried out remove impurity respectively, clean, mix, soaked into 1 hour with the 75% ethanol stirring of crude drug 1 times of weight, in the percolation jar of packing into; Use 75% ethanol percolate extraction 3 times of crude drug 10,8,6 times of weight respectively, collect and merge extractive liquid,, filter; With extracting solution feed absorption, washing, the flow velocity of resolving is 0.1-10 times of bed volume/hour the D101 macroporous adsorbent resin; Earlier use water washing,, resolve with 60% ethanol at last again with 20% washing with alcohol; Collect ethanol liquid, reclaiming ethanol and it is condensed into relative density is 1.25 concentrated solution; Add conventional adjuvant,, process Tibetan medicine syrup of the present invention according to common process.
The preparation that [embodiment 6] Tibetan medicine soup of the present invention looses
Cream 100g, Fructus Phyllanthi 130g such as ZANGJINJIER 150g, BAXIAGA 100g, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.) 100g, Herba Corydalis 100g, Song Sheng;
Cream, Fructus Phyllanthi medical materials such as ZANGJINJIER, BAXIAGA, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.), Herba Corydalis, Song Sheng are carried out remove impurity, cleaning, drying respectively, be ground into coarse powder, sieve, mixing is processed Tibetan medicine soup of the present invention and is loose.
The discriminating of [embodiment 7] Tibetan medicine hard capsule of the present invention
Cream 100g, Fructus Phyllanthi 130g such as ZANGJINJIER 150g, BAXIAGA 100g, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.) 100g, Herba Corydalis 100g, Song Sheng;
Cream, Fructus Phyllanthi medical materials such as ZANGJINJIER, BAXIAGA, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.), Herba Corydalis, Song Sheng are carried out remove impurity respectively, clean, mix, extract 3 times with 75% alcohol dipping of crude drug 10,8,6 times of weight respectively, flooded 12 hours at every turn; Collect and merge extractive liquid; Filter, with extracting solution feed absorption, washing, the flow velocity of resolving is 0.1-10 times of bed volume/hour the D101 macroporous adsorbent resin, use earlier water washing; Again with 20% washing with alcohol; Resolve with 60% ethanol at last, collect ethanol liquid, reclaiming ethanol and it is condensed into relative density is 1.25 concentrated solution; Add conventional adjuvant,, process Tibetan medicine hard capsule of the present invention according to common process.
The preparation of [embodiment 8] Tibetan medicine tablet of the present invention
Cream 100g, Fructus Phyllanthi 130g such as ZANGJINJIER 150g, BAXIAGA 100g, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.) 100g, Herba Corydalis 100g, Song Sheng;
Cream, Fructus Phyllanthi medical materials such as ZANGJINJIER, BAXIAGA, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.), Herba Corydalis, Song Sheng are carried out remove impurity respectively, clean, mix, and the decocting with crude drug 10,8,6 times of weight boils 3 times respectively, decocts 1 hour at every turn; Collect and merge extractive liquid; Filter, it is 1.25 concentrated solution that extracting solution is condensed into relative density, adds conventional adjuvant; According to common process, process Tibetan medicine tablet of the present invention.
The preparation of [embodiment 9] Tibetan medicine drop pill of the present invention
Cream 80g, Fructus Phyllanthi 80g such as ZANGJINJIER 180g, BAXIAGA 180g, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.) 80g, Herba Corydalis 80g, Song Sheng;
Cream, Fructus Phyllanthi medical materials such as ZANGJINJIER, BAXIAGA, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.), Herba Corydalis, Song Sheng are carried out remove impurity respectively, clean, mix, extract 3 times with 70% alcohol dipping of crude drug 12,10,8 times of weight respectively, flooded 16 hours at every turn; Collect and merge extractive liquid; Filter, it is 1.35 concentrated solution that extracting solution is condensed into relative density, adds conventional adjuvant; According to common process, process Tibetan medicine drop pill of the present invention.
The discriminating of [embodiment 10] Tibetan medicine hard capsule of the present invention
Cream 140g, Fructus Phyllanthi 170g such as ZANGJINJIER 190g, BAXIAGA 140g, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.) 140g, Herba Corydalis 140g, Song Sheng;
Cream, Fructus Phyllanthi medical materials such as ZANGJINJIER, BAXIAGA, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.), Herba Corydalis, Song Sheng are carried out remove impurity, clean, dry respectively, be ground into coarse powder, sieve, mixing adds conventional adjuvant, according to common process, processes Tibetan medicine hard capsule of the present invention.
The particulate discriminating of [embodiment 11] Tibetan medicine of the present invention
Cream 140g, Fructus Phyllanthi 170g such as ZANGJINJIER 190g, BAXIAGA 140g, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.) 140g, Herba Corydalis 140g, Song Sheng;
Cream, Fructus Phyllanthi medical materials such as ZANGJINJIER, BAXIAGA, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.), Herba Corydalis, Song Sheng are carried out remove impurity respectively, clean, mix, and the decocting with crude drug 12,10,8 times of weight boils 3 times respectively, decocts 1.5 hours at every turn; Collect and merge extractive liquid; Filter, it is 1.35 concentrated solution that extracting solution is condensed into relative density, adds conventional adjuvant; According to common process, process Tibetan medicine granule of the present invention.
The preparation of [embodiment 12] Tibetan medicine pill of the present invention
Cream 140g, Fructus Phyllanthi 170g such as ZANGJINJIER 190g, BAXIAGA 140g, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.) 140g, Herba Corydalis 140g, Song Sheng;
Cream, Fructus Phyllanthi medical materials such as ZANGJINJIER, BAXIAGA, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.), Herba Corydalis, Song Sheng are carried out remove impurity respectively, clean, mix; Use 75% alcohol reflux 3 times of crude drug 10,8,6 times of weight respectively; The each extraction 1 hour; Collect and merge extractive liquid,, filter, it is 1.25 concentrated solution that extracting solution is condensed into relative density; Add conventional adjuvant,, process Tibetan medicine pill of the present invention according to common process.
The preparation of [embodiment 13] Tibetan medicine controlled release preparation of the present invention
Cream 150g, Fructus Phyllanthi 80g such as ZANGJINJIER 200g, BAXIAGA 50g, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.) 150g, Herba Corydalis 50g, Song Sheng;
Cream, Fructus Phyllanthi medical materials such as ZANGJINJIER, BAXIAGA, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.), Herba Corydalis, Song Sheng are carried out remove impurity respectively, clean, mix, extract 3 times, flooded 8 hours at every turn with 65% alcohol dipping of crude drug 10 times of weight; Collect and merge extractive liquid; Filter, with extracting solution feed absorption, washing, the flow velocity of resolving is 0.1-10 times of bed volume/hour cation exchange resin, use earlier water washing; Again with 30% washing with alcohol; Resolve with 70% ethanol at last, collect ethanol liquid, reclaiming ethanol and it is condensed into relative density is 1.15 concentrated solution; Add conventional adjuvant,, process Tibetan medicine controlled release preparation of the present invention according to common process.
The discriminating that [embodiment 14] Tibetan medicine soup of the present invention looses
40g, Fructus Phyllanthi 90g such as ZANGJINJIER 110g, BAXIAGA 60g, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.) 60g, Herba Corydalis 60g, Song Sheng;
ZANGJINJIER, BAXIAGA, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.), Herba Corydalis, Song Sheng etc., Fructus Phyllanthi medical material are carried out remove impurity, clean, dry respectively, be ground into coarse powder, sieve, mixing adds conventional adjuvant, according to common process, processes Tibetan medicine soup of the present invention and looses.
The preparation of [embodiment 15] Tibetan medicine hard capsule of the present invention
100g, Fructus Phyllanthi 130g such as ZANGJINJIER 150g, BAXIAGA 100g, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.) 100g, Herba Corydalis 100g, Song Sheng;
ZANGJINJIER, BAXIAGA, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.), Herba Corydalis, Song Sheng etc., Fructus Phyllanthi medical material are carried out remove impurity respectively, clean, mix, extract 3 times, flooded 12 hours at every turn with 75% alcohol dipping of crude drug 10 times of weight; Collect and merge extractive liquid; Filter, with extracting solution feed absorption, washing, the flow velocity of resolving is 0.1-10 times of bed volume/hour the D101 macroporous adsorbent resin, use earlier water washing; Again with 20% washing with alcohol; Resolve with 60% ethanol at last, collect ethanol liquid, reclaiming ethanol and it is condensed into relative density is 1.25 concentrated solution; Add conventional adjuvant,, process Tibetan medicine hard capsule of the present invention according to common process.
The preparation of [embodiment 16] Tibetan medicine tablet of the present invention
180g, Fructus Phyllanthi 170g such as ZANGJINJIER 190g, BAXIAGA 140g, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.) 140g, Herba Corydalis 140g, Song Sheng;
ZANGJINJIER, BAXIAGA, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.), Herba Corydalis, Song Sheng etc., Fructus Phyllanthi medical material are carried out remove impurity respectively, clean, mix, boil 3 times, decocted 1 hour at every turn with the decocting of crude drug 10 times of weight; Collect and merge extractive liquid; Filter, it is 1.25 concentrated solution that extracting solution is condensed into relative density, adds conventional adjuvant; According to common process, process Tibetan medicine tablet of the present invention.
Claims (10)
1. a Tibetan medicinal preparation of treating essential hypertension is processed by following crude drug: 30-200 weight portion, Fructus Phyllanthi 80-180 weight portion such as cream 50-150 weight portion such as ZANGJINJIER 100-200 weight portion, BAXIAGA 50-150 weight portion, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.) 50-150 weight portion, Herba Corydalis 50-150 weight portion, Song Sheng or Song Sheng.
2. Tibetan medicinal preparation as claimed in claim 1; It is characterized in that, process: 50-150 weight portion, Fructus Phyllanthi 100-150 weight portion such as cream 80-120 weight portion such as ZANGJINJIER 120-180 weight portion, BAXIAGA 80-120 weight portion, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.) 80-120 weight portion, Herba Corydalis 80-120 weight portion, Song Sheng or Song Sheng by following crude drug.
3. Tibetan medicinal preparation as claimed in claim 1 is characterized in that being processed by following crude drug: 100 weight portions, Fructus Phyllanthi 130 weight portions such as cream 100 weight portions such as ZANGJINJIER 150 weight portions, BAXIAGA 100 weight portions, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.) 100 weight portions, Herba Corydalis 100 weight portions, Song Sheng or Song Sheng.
4. Tibetan medicinal preparation as claimed in claim 1 is characterized in that, said preparation is pill, tablet, capsule, oral liquid, syrup, granule, powder, drop pill, slow releasing preparation or controlled release preparation.
5. the application of each described Tibetan medicinal preparation of claim 1～4 on the preparation antihypertensive drugs.
6. the method for preparing of the described Tibetan medicinal preparation of claim 1 comprises cream such as ZANGJINJIER, BAXIAGA, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.), Herba Corydalis, Song Sheng or Song Sheng etc., the remove impurity respectively of Fructus Phyllanthi crude drug, cleaning, drying, is ground into coarse powder, sieves, and mixing promptly gets.
7. the method for preparing of the said Tibetan medicinal preparation of claim 1; Comprise cream such as ZANGJINJIER, BAXIAGA, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.), Herba Corydalis, Song Sheng or Song Sheng etc., Fructus Phyllanthi crude drug are carried out remove impurity respectively, clean, mix; With the 50-90% alcohol reflux of crude drug 5-15 times of weight 1-3 time, extracted 0.5-2.5 hour at every turn, collect and merge extractive liquid; Filter, extracting solution is condensed into the concentrated solution that relative density is 1.0-1.5; Or with extracting solution feed absorption, washing, the flow velocity of resolving is 0.1-10 times of bed volume/hour D101 or D101B or XDA-1 or XDA-1B or HPD-300 macroporous adsorbent resin or cation exchange resin; Use earlier water washing; Again with the 10-30% washing with alcohol; Resolve with 50-70% ethanol at last, collect ethanol liquid, reclaim ethanol and it is condensed into the concentrated solution that relative density is 1.0-1.5; Add the adjuvant of pharmaceutically accepting, process said Tibetan medicinal preparation.
8. the method for preparing of the said Tibetan medicinal preparation of claim 1; Comprise cream such as ZANGJINJIER, BAXIAGA, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.), Herba Corydalis, Song Sheng or Song Sheng etc., the remove impurity respectively of Fructus Phyllanthi medical material, clean, mix, stir infiltration 0.5-2 hour with the 50-90% ethanol of crude drug 0.5-1.5 times of weight, in the percolation jar of packing into; With the 50-90% ethanol percolate extraction of crude drug 5-15 times of weight 2-4 time; Collect and merge extractive liquid,, filter, extracting solution is condensed into the concentrated solution that relative density is 1.0-1.5; Or with extracting solution feed absorption, washing, the flow velocity of resolving is 0.1-10 times of bed volume/hour D101 or D101B or XDA-1 or XDA-1B or HPD-300 macroporous adsorbent resin or cation exchange resin; Use earlier water washing; Again with the 10-30% washing with alcohol; Resolve with 50-70% ethanol at last, collect ethanol liquid, reclaim ethanol and it is condensed into the concentrated solution that relative density is 1.0-1.5; Add the adjuvant of pharmaceutically accepting, process said Tibetan medicinal preparation.
9. the method for preparing of the said Tibetan medicinal preparation of claim 1; Comprise cream such as ZANGJINJIER, BAXIAGA, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.), Herba Corydalis, Song Sheng or Song Sheng etc., the remove impurity respectively of Fructus Phyllanthi medical material, clean, mix; 50-90% alcohol dipping with crude drug 5-15 times of weight is extracted 2-4 time, floods 8-16 hour at every turn, collects and merge extractive liquid; Filter, extracting solution is condensed into the concentrated solution that relative density is 1.0-1.5; Or with extracting solution feed absorption, washing, the flow velocity of resolving is 0.1-10 times of bed volume/hour D101 or D101B or XDA-1 or XDA-1B or HPD-300 macroporous adsorbent resin or cation exchange resin; Use earlier water washing; Again with the 10-30% washing with alcohol; Resolve with 50-70% ethanol at last, collect ethanol liquid, reclaim ethanol and it is condensed into the concentrated solution that relative density is 1.0-1.5; Add the adjuvant of pharmaceutically accepting, process said Tibetan medicinal preparation.
10. the method for preparing of the said Tibetan medicinal preparation of claim 1 comprises cream such as ZANGJINJIER, BAXIAGA, Lagotis clarkei Hook. F (Lagotis brevituba Maxim.), Herba Corydalis, Song Sheng or Song Sheng etc., Fructus Phyllanthi is carried out remove impurity respectively, cleans, mixes, and boils 2-4 time with the decocting of crude drug 5-15 times of weight; The each decoction 0.5-2.5 hour; Collect and merge extractive liquid,, filter, extracting solution is condensed into the concentrated solution that relative density is 1.0-1.5; Add the adjuvant of pharmaceutically accepting, process said Tibetan medicinal preparation.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103735575A (en) * | 2014-01-26 | 2014-04-23 | 青岛科技大学 | Rhamnella gilgitica extract and preparation method and application thereof |
| CN107582862A (en) * | 2017-11-07 | 2018-01-16 | 平措绕吉 | For treating the medicine and its processing method of angiocardiopathy |
| CN107714889A (en) * | 2017-10-12 | 2018-02-23 | 佛山市赛普斯科技服务有限公司 | A kind of Chinese herbal medicine formula of heat-clearing hypotensive and preparation method thereof |
| CN115969895A (en) * | 2023-01-16 | 2023-04-18 | 青海民族大学 | Application of Tibetan medicine golden cypress caragana in preparation of altitude erythrocytosis medicine |
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| CN101391081A (en) * | 2008-11-11 | 2009-03-25 | 南京同仁堂药业有限责任公司 | Medicine composition for treating qi and blood derangement of essential hypertension and preparation method thereof |
| CN102590430A (en) * | 2012-01-17 | 2012-07-18 | 西藏奇正藏药股份有限公司 | Method for detecting liuwei caragana preparation |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101391081A (en) * | 2008-11-11 | 2009-03-25 | 南京同仁堂药业有限责任公司 | Medicine composition for treating qi and blood derangement of essential hypertension and preparation method thereof |
| CN102590430A (en) * | 2012-01-17 | 2012-07-18 | 西藏奇正藏药股份有限公司 | Method for detecting liuwei caragana preparation |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103735575A (en) * | 2014-01-26 | 2014-04-23 | 青岛科技大学 | Rhamnella gilgitica extract and preparation method and application thereof |
| CN107714889A (en) * | 2017-10-12 | 2018-02-23 | 佛山市赛普斯科技服务有限公司 | A kind of Chinese herbal medicine formula of heat-clearing hypotensive and preparation method thereof |
| CN107582862A (en) * | 2017-11-07 | 2018-01-16 | 平措绕吉 | For treating the medicine and its processing method of angiocardiopathy |
| CN115969895A (en) * | 2023-01-16 | 2023-04-18 | 青海民族大学 | Application of Tibetan medicine golden cypress caragana in preparation of altitude erythrocytosis medicine |
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Application publication date: 20121114 |