CN102757901A - Microorganism collection method in ecological environment survey - Google Patents
Microorganism collection method in ecological environment survey Download PDFInfo
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- CN102757901A CN102757901A CN2012101351059A CN201210135105A CN102757901A CN 102757901 A CN102757901 A CN 102757901A CN 2012101351059 A CN2012101351059 A CN 2012101351059A CN 201210135105 A CN201210135105 A CN 201210135105A CN 102757901 A CN102757901 A CN 102757901A
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- 238000000034 method Methods 0.000 title claims abstract description 43
- 244000005700 microbiome Species 0.000 title abstract 5
- 241000233866 Fungi Species 0.000 claims abstract description 17
- 241000894006 Bacteria Species 0.000 claims description 122
- 239000002689 soil Substances 0.000 claims description 58
- 241000196324 Embryophyta Species 0.000 claims description 44
- 239000011159 matrix material Substances 0.000 claims description 41
- 239000004744 fabric Substances 0.000 claims description 13
- 239000000463 material Substances 0.000 claims description 11
- 238000004659 sterilization and disinfection Methods 0.000 claims description 10
- 239000004677 Nylon Substances 0.000 claims description 7
- 229920001778 nylon Polymers 0.000 claims description 7
- 238000007873 sieving Methods 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 4
- 238000012216 screening Methods 0.000 abstract description 7
- 238000007789 sealing Methods 0.000 abstract description 2
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 238000004806 packaging method and process Methods 0.000 abstract 1
- 235000004047 Amorpha fruticosa Nutrition 0.000 description 12
- 240000002066 Amorpha fruticosa Species 0.000 description 12
- 239000012174 chinese wax Substances 0.000 description 11
- 241000124033 Salix Species 0.000 description 8
- ZZUFCTLCJUWOSV-UHFFFAOYSA-N furosemide Chemical group C1=C(Cl)C(S(=O)(=O)N)=CC(C(O)=O)=C1NCC1=CC=CO1 ZZUFCTLCJUWOSV-UHFFFAOYSA-N 0.000 description 8
- 230000012010 growth Effects 0.000 description 8
- 238000005065 mining Methods 0.000 description 8
- 239000002068 microbial inoculum Substances 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 241000773655 Ambispora fennica Species 0.000 description 5
- 239000003245 coal Substances 0.000 description 4
- 238000005260 corrosion Methods 0.000 description 4
- 238000011065 in-situ storage Methods 0.000 description 4
- 238000011084 recovery Methods 0.000 description 4
- 241000287828 Gallus gallus Species 0.000 description 3
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- 238000007605 air drying Methods 0.000 description 3
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- 238000013461 design Methods 0.000 description 3
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- 241001185310 Symbiotes <prokaryote> Species 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
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- 238000009825 accumulation Methods 0.000 description 1
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- 230000007812 deficiency Effects 0.000 description 1
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- 238000005516 engineering process Methods 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
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- 241001478887 unidentified soil bacteria Species 0.000 description 1
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a microorganism collection method in an ecological environment survey. The microorganism for the microorganism collection method is arbuscular mycorrhizal fungi. The collection method comprises the following steps: manufacturing a collection culture bag, preparing experimental matrixes, and packaging the experimental matrixes to the culture bag and sealing, and launching the culture bag. By the microorganism collection method in the ecological environment survey, the culture bag can be enriched in spore and mycelium, and the screening range is narrowed greatly and the workload is reduced.
Description
Technical field
The present invention relates to a kind of mikrobe acquisition method, be specifically related to the mikrobe acquisition method in a kind of ecotope prospecting.
Background technology
Bush mycorrhizal fungi (AMF) is a kind of ubiquitous endosymbiosis fungi, and it can form symbiote with the terrestrial plant more than 80%.Bush mycorrhizal fungi is playing crucial effects aspect the resistance of keeping the growth of stablizing, promote plant of the plant rhizosphere ecosystem, raising host plant and the raising degenerated soil quality.Bush mycorrhizal fungi is made up of mycorhiza spore (really), Cong Zhiti, vesicle, mycelia, and they can be as propagulum.At present, the separation and purification of mycorhiza mainly adopts spore as donor, and microsporocrap, spore and autochthonal helper utilize the wet screening collection usually in the soil.Utilize single spore as the bacterium source many unfavorable factors to be arranged, at first, it is medium that spore infects speed, and the accumulation of culture propagation body quantity is slower; Secondly, in the soil that wet screening process miospore amount is few because the interference of grogs and organic granular is not easy to separate spore; Volume possibly lost than big or smaller particles in the wet screening process simultaneously.Though utilize spore can obtain purebred as propagulum; But the microbial inoculum by single bacterium source produces is difficult to realize maximization in the occurring in nature effect; The change of field environment, the antagonism property between the bacterial classification and host plant all can impact single bacterium source the selectivity of bacterial classification etc., and the microbial inoculum field that produces thus is difficult to apply.Bush mycorrhizal fungi is an obligate biotroph bacterial parasite; Only after forming mycorhiza on the live plant root, could breed into spore and sporocarp; Be difficult to duplicate rhizosphere environment between mycorhiza and the host plant in the laboratory; In addition the quantity of mycorhiza propagulum in soil, survival time, active size, to infect the opposite sex that is wanting in such as speed big, indoor be difficult to filter out be fit to open-air growth mycorhiza flora.Therefore, along with mycorhiza under the more and more wider applicable cases in aspects such as ECOLOGICAL REHABILITATION OF MINING AREAS, soil remediation and agro-ecology fertility, open-air in-situ acquisition arbuscular mycorrhiza flora with utilize composite flora to produce most important that microbial inoculum just becomes.
Summary of the invention
To the deficiency of bush mycorrhizal fungi acquisition method in the prior art, the present invention provides the mikrobe acquisition method in a kind of ecotope prospecting.
The present invention provides the mikrobe acquisition method in a kind of ecotope prospecting, and said mikrobe is a bush mycorrhizal fungi, and said acquisition method may further comprise the steps:
(1) make to gather the bacterium bag: the cloth of choosing the aperture and be 30 μ m is as the bacterium bag material; Process the bacterium bag with this bacterium bag material; One end of said bacterium bag is provided with opening;
(2) preparation supplies examination matrix: supplying examination matrix is the soil of target acquisition zone, said soil is crossed the sieve that the aperture is 1mm, soil disinfection and drying for standby after will sieving then;
(3) confession that obtains in the step (2) examination matrix is packed in the bacterium bag of making in the step (1), load finish after, with the closure of openings of said bacterium bag;
(4) the bacterium bag that supplies to try matrix that is equipped with that obtains in the step (3) is placed near the host plant root, after placement is accomplished, mulching soil.
Preferably, said mikrobe acquisition method specifically may further comprise the steps:
(1) make to gather the bacterium bag: the nylon cloth of choosing the aperture and be 30 μ m is as the bacterium bag material; Process the bacterium bag with this bacterium bag material; The length of said bacterium bag is that 5～15cm, width are that 4～12cm, thickness are 2～5cm; One end of bacterium bag is provided with opening;
(2) preparation supplies examination matrix: supplying examination matrix is the soil of target acquisition zone, said soil is crossed the sieve that the aperture is 1mm, soil disinfection and drying for standby after will sieving then;
(3) confession that obtains in the step (2) examination matrix is packed in the bacterium bag of making in the step (1), the amount of fill of each bacterium bag is 50～150g, load finish after, with the closure of openings of said bacterium bag;
(4) the bacterium bag that confession examination matrix is housed that obtains in the step (3) is placed near the host plant root, the modes of emplacement of said bacterium bag is vertical the placement, after placement is accomplished, and mulching soil.
In the step (4), the bacterium bag that supplies examination matrix is housed, and placement comprises near the host plant root: the soil that will press close to said host plant root cuts out, and supplies the bacterium bag of examination matrix to place near the host plant root said being equipped with then.
In the step (1), said bacterium bag has anti-corrosion function, promptly in one of host plant growth season, and this bacterium bag imputrescibility in soil.
Preferably, in the step (1), the length of said bacterium bag is that 10cm, width are that 8cm, thickness are 3cm; And in the step (3), the amount of fill of each bacterium bag is 100g.
Preferably, in the step (1), an end of said bacterium bag has traction rope.During collection, said traction rope ties up to the stem portion of host plant, is convenient to reclaim said bacterium bag.
It will be understood by those skilled in the art that for ease said traction rope preferably is arranged on the end that said bacterium bag has opening.
Further, in order to make said bacterium bag vertically to place, said traction rope more preferably is arranged on the middle part of an end of opening.
In the step (2), said sterilization can adopt any way well known by persons skilled in the art to carry out; Preferably, the high temperature and high pressure steam sterilization is adopted in said sterilization.
In the step (3), the sealing of said opening can adopt any way well known by persons skilled in the art to carry out, and for example adopts the mode of sewing up with closure of openings.
Mikrobe acquisition method in the ecotope prospecting provided by the invention further may further comprise the steps:
After one of said host plant is grown season, remove the soil on said bacterium bag top, take out said bacterium bag, then the index that comprises spore density, mycelial density, mycelia length in the said bacterium bag is measured.These measuring methods can adopt any method well known by persons skilled in the art to carry out, and for example spore density adopts wet screening tilt-pour process, mycelial density to adopt the Box junction method.
Mikrobe acquisition method in the ecotope prospecting of the present invention, utilizing the aperture is nylon cloth making bacterium bag collection mycelium and the spore of 30 μ m.The mycelium diameter is 3～7 μ m, and mycelium can freely get into the bacterium bag like this, and very difficult entering such as host plant root system, in such cases, the local root system of host plant just forces its symbiote mycorhiza and appurtenant thereof to get into the bacterium bag for obtaining nutrient.Confession examination matrix in the bacterium bag is sieved and is sterilized, and has to a certain degree improved the quality that supplies examination matrix, has got rid of the interference of other mikrobe simultaneously, more helps mycorhiza and appendicular growth thereof.In the prior art, arbuscular mycorrhiza separates every kind to be needed to gather soil sample 1.5～2kg, from these soil samples, is separating spore then, and workload is often bigger like this.Utilize the mikrobe acquisition method in the ecotope prospecting of the present invention to gather the difficult problem that arbuscular mycorrhiza has largely solved open-air arbuscular mycorrhiza collection; Confession examination matrix in the bacterium bag 100g that only has an appointment; The physicochemical character and the quality thereof that supply examination matrix in the bacterium bag have been changed simultaneously artificially; More help spore and mycelial enrichment, dwindled the screening scope so to a great extent and reduced workload.Utilize the mikrobe acquisition method in the ecotope prospecting of the present invention; Wherein said bacterium bag can enrichment spore and mycelium; And with its bacterium source that produces as microbial inoculum; This just provides technical support for the open-air arbuscular mycorrhiza microbial inoculum of producing, and also applies in different zones for microbial inoculum and provides fundamental basis.
Embodiment
Following embodiment only is used to explain the present invention, and unrestricted the present invention.
Nylon cloth is available from Chongwen, Beijing development wire cloth shop, and the aperture is 30 μ m.
Spore density adopts the wet screening tilt-pour process to measure; Mycelial density adopts the Box junction method to measure.
Embodiment 1: (in-situ acquisition of subsidence area, live chickens rabbit ore deposit, Yulin bush mycorrhizal fungi)
The nylon cloth of choosing the aperture and be 30 μ m is as the bacterium bag material.This bacterium bag has anti-corrosion function, in a growth season of host plant, and this bacterium bag imputrescibility in soil.The length of bacterium bag is that 10cm, width are 8cm, and the thickness of bacterium bag is 3cm; One end of bacterium bag is provided with opening; The middle part of opening end is provided with traction rope.
Supply examination matrix collecting location to be positioned at the Da Liu of Shenmu County, Yulin tower town Gao Jiapan; Belong to live chickens rabbit ore deposit, Shen Dong mining area and adopt the sky subsidence area; Supplying examination matrix is the degenerated soil of this subsidence area, and the aperture is the sieve of 1mm excessively, and the soil after sieving is sterilized (121 ℃ through high temperature and high pressure steam before the above-mentioned bacterium bag of packing into; 2h), natural air drying.
Above-mentioned confession examination matrix is packed in the above-mentioned bacterium bag, and each bacterium is packed to go into to supply examination matrix 100g, load finish after, the opening of said bacterium bag is sewed up.
Host plant is a false indigo, false indigo be selected from subsidence area, live chickens rabbit ore deposit mikrobe reclaim the demonstration base.Supplying the average plant height of examination false indigo is 43.3cm, and the hat width of cloth is 26cm.
Tested on April 17th, 2011 and carry out, the soil thawing in Shen Dong mining area at this moment, host plant is in the season of turning green.20 repetitions of test design promptly select 20 strain false indigos as host plant, choose false indigo according to S type curve, and selected 20 strain false indigo sizes are close.The soil that to press close to the false indigo root cuts out; Supply the bacterium bag of examination matrix vertically to place near the false indigo root above-mentioned being equipped with then, the placement degree of depth is 15cm, mulching soil after placement is accomplished; And the traction rope of bacterium bag tied up to the stem portion of false indigo, so that reclaim the bacterium bag.
Fetch said bacterium bag at the beginning of 10 months and gather the host plant rhizosphere soil.October is study area freezing season just, and the plant in this district stops growing, and this test is a growth season of host plant.Supplying to try matrix in the bacterium bag to the rhizosphere soil gathered and recovery measures.Host plant rhizosphere soil miospore density, mycelial density and 100g soil bacteria filament length degree are respectively 15/g, 3.19mg
-1And 319m.Spore density, mycelial density and mycelia length in the bacterium bag that reclaims are measured, and the spore density in the bacterium bag is 6.04mg up to 33/g, mycelial density
-1, total mycelia length is 604m in the bacterium bag.Spore density and mycelial density in the said bacterium bag exceed 18/g and 2.85mg respectively than false indigo rhizosphere soil
-1Explain that mikrobe acquisition method of the present invention can improve the collection capacity and the picking rate of open-air bush mycorrhizal fungi greatly.
Embodiment 2: (in-situ acquisition of subsidence area, Lian Ta ore deposit bush mycorrhizal fungi is mended in the Shen Dong mining area)
The nylon cloth of choosing the aperture and be 30 μ m is as the bacterium bag material.This bacterium bag has anti-corrosion function, in a growth season of host plant, and this bacterium bag imputrescibility in soil.The length of bacterium bag is that 10cm, width are 8cm, and the thickness of bacterium bag is 3cm; One end of bacterium bag is provided with opening; The middle part of opening end is provided with traction rope.
Experimental field be selected in the Shen Dong mining area and mend Lian Takuang, supplying examination matrix be this subsidence area, ore deposit degenerated soil, and to cross the aperture be the sieve of 1mm, the soil after sieving through high temperature and high pressure steam sterilize (121 ℃, 2h), natural air drying.
Above-mentioned confession examination matrix is packed in the above-mentioned bacterium bag, and each bacterium is packed to go into to supply examination matrix 100g, load finish after, the opening of said bacterium bag is sewed up.
Host plant is a salix monogolica.The average plant height of salix monogolica is 136.5cm, and the hat width of cloth is 96.2cm.
Tested on April 18th, 2011 and carry out, the soil thawing in Shen Dong mining area at this moment, host plant is in the season of turning green.20 repetitions of test design promptly select 20 strain salix monogolicas as host plant, choose false indigo according to S type curve, and selected 20 strain false indigo sizes are close.The soil that to press close to the salix monogolica root cuts out, and supplies the bacterium bag of examination matrix vertically to place near the salix monogolica root above-mentioned being equipped with then, and the placements degree of depth is 15cm, place accomplish after mulching soil, and the traction rope of bacterium bag tied up to the stem portion of salix monogolica, so that recovery bacterium bag.
Fetch said bacterium bag at the beginning of 10 months and gather the host plant rhizosphere soil, mycelia length in the examination matrix of the confession in the bacterium bag that reclaims and salix monogolica rhizosphere soil miospore density, mycelial density and the 100g soil is measured.As a result, spore density, mycelial density and the mycelia length in the bacterium bag reaches 21/g, 4.74mg respectively
-1And 4.74m, and rhizosphere soil miospore density, mycelial density and mycelia length are respectively 11/g, 1.91mg
-1And 1.91m.Spore density and mycelial density in the said bacterium bag exceed 10/g and 2.83mg than salix monogolica rhizosphere soil
-1Explain that mikrobe acquisition method of the present invention can significantly improve the acquisition rate of open-air bush mycorrhizal fungi, shorten the acquisition time of bush mycorrhizal fungi.
Embodiment 3: (the Ningxia coal gangue hill is reclaimed and distinguished the in-situ acquisition of bush mycorrhizal fungi)
The nylon cloth of choosing the aperture and be 30 μ m is as the bacterium bag material.This bacterium bag has anti-corrosion function, in a growth season of host plant, and this bacterium bag imputrescibility in soil.The length of bacterium bag is that 10cm, width are 8cm, and the thickness of bacterium bag is 3cm; One end of bacterium bag is provided with opening; The middle part of opening end is provided with traction rope.
The test place is Da Wukou washery coal gangue hill in Ningxia, and this ground is positioned at Ningxia Hui Autonomous Region's Shizuishan City, dry climate district, temperate zone in belonging to, and precipitation is rare and concentrate, and illumination is sufficient, evaporates strongly, dry air, the utmost point is unfavorable for the growth of plant.This is experimental field on the point selection coal gangue hill, the coal gangue hill upper berth the thick river sand of 0.8～1m as growth matrix, the test site is China Mining Univ. (Beijing) mining area mikrobe demonstration base of reclaiming.
Supplying examination matrix is discarded chiltern on the waste dump, and to cross the aperture be the sieve of 1mm, sieve after the high temperature and high pressure steam sterilization (121 ℃, 2h), natural air drying.
Above-mentioned confession examination matrix is packed in the above-mentioned bacterium bag, and each bacterium is packed to go into to supply examination matrix 100g, load finish after, the opening of said bacterium bag is sewed up.
Host plant is local pioneer plant Chinese wax, and the average plant height of Chinese wax is 217cm, and the hat width of cloth is 151cm.
Tested on April 25th, 2011 and carry out, the thawing of the geographic soil in Ningxia this moment, host plant is in the season of turning green.20 repetitions of test design promptly select 20 strain Chinese wax as host plant, choose Chinese wax according to S type curve, and selected 20 strain Chinese wax sizes are close.The soil that to press close to the Chinese wax root cuts out, and supplies the bacterium bag of examination matrix vertically to place near the Chinese wax root above-mentioned being equipped with then, and the placements degree of depth is 15cm, place accomplish after mulching soil, and the traction rope of bacterium bag tied up to the stem portion of Chinese wax, so that recovery bacterium bag.
Fetch the bacterium bag at the beginning of 10 months and gather the host plant rhizosphere soil, mycelia length in the confession examination matrix in the recovery bacterium bag and Chinese wax rhizosphere soil spore density, mycelial density and the 100g soil is measured.As a result, bacterium bag Endospore density, mycelial density and mycelia length are respectively 28/g, 5.66mg
-1And 566m, and Chinese wax rhizosphere soil miospore density, mycelial density and mycelia length are respectively 13/g, 2.81mg
-1And 281m.Spore density and mycelial density in the said bacterium bag exceed 15/g and 2.85mg than Chinese wax rhizosphere soil
-1Explain that mikrobe acquisition method of the present invention can significantly improve the acquisition rate of open-air bush mycorrhizal fungi, shorten the acquisition time of bush mycorrhizal fungi.
It will be understood by those skilled in the art that under the instruction of this specification sheets, can make some modifications or variation the present invention.These modifications and change also should be within claim of the present invention institute restricted portion.
Claims (8)
1. the mikrobe acquisition method during an ecotope is reconnoitred, said mikrobe is a bush mycorrhizal fungi, said acquisition method may further comprise the steps:
(1) make to gather the bacterium bag: the cloth of choosing the aperture and be 30 μ m is as the bacterium bag material; Process the bacterium bag with this bacterium bag material; One end of said bacterium bag is provided with opening;
(2) preparation supplies examination matrix: supplying examination matrix is the soil of target acquisition zone, said soil is crossed the sieve that the aperture is 1mm, soil disinfection and drying for standby after will sieving then;
(3) confession that obtains in the step (2) examination matrix is packed in the bacterium bag of making in the step (1), load finish after, with the closure of openings of said bacterium bag;
(4) the bacterium bag that supplies to try matrix that is equipped with that obtains in the step (3) is placed near the host plant root, after placement is accomplished, mulching soil.
2. mikrobe acquisition method according to claim 1 is characterized in that, said acquisition method specifically may further comprise the steps:
(1) make to gather the bacterium bag: the nylon cloth of choosing the aperture and be 30 μ m is as the bacterium bag material; Process the bacterium bag with this bacterium bag material; The length of said bacterium bag is that 5～15cm, width are that 4～12cm, thickness are 2～5cm; One end of bacterium bag is provided with opening;
(2) preparation supplies examination matrix: supplying examination matrix is the soil of target acquisition zone, said soil is crossed the sieve that the aperture is 1mm, soil disinfection and drying for standby after will sieving then;
(3) confession that obtains in the step (2) examination matrix is packed in the bacterium bag of making in the step (1), the amount of fill of each bacterium bag is 50～150g, load finish after, with the closure of openings of said bacterium bag;
(4) the bacterium bag that confession examination matrix is housed that obtains in the step (3) is placed near the host plant root, the modes of emplacement of said bacterium bag is vertical the placement, after placement is accomplished, and mulching soil.
3. mikrobe acquisition method according to claim 2; It is characterized in that; In the step (4); The bacterium bag that supplies examination matrix is housed, and placement comprises near the host plant root: the soil that will press close to said host plant root cuts out, and supplies the bacterium bag of examination matrix to place near the host plant root said being equipped with then.
4. mikrobe acquisition method according to claim 3 is characterized in that, in the step (1), the length of said bacterium bag is that 1Ocm, width are that 8cm, thickness are 3cm; And in the step (3), the amount of fill of each bacterium bag is 100g.
5. mikrobe acquisition method according to claim 4 is characterized in that, in the step (1), an end of said bacterium bag has traction rope.
6. mikrobe acquisition method according to claim 5 is characterized in that, said traction rope is arranged on the end that said bacterium bag has opening, and is arranged on said middle part with end of opening.
7. mikrobe acquisition method according to claim 6 is characterized in that, in the step (2), said sterilization is to adopt the high temperature and high pressure steam sterilization.
8. according to each described mikrobe acquisition method of claim 1～7, it is characterized in that said mikrobe acquisition method further may further comprise the steps:
After one of said host plant is grown season, remove the soil on said bacterium bag top, take out said bacterium bag, then the index that comprises spore density, mycelial density, mycelia length in the said bacterium bag is measured.
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| CN103396980A (en) * | 2013-06-18 | 2013-11-20 | 中国矿业大学(北京) | Field collection method of arbuscular mycorrhizal spores by rapid gathering |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102268391A (en) * | 2011-07-09 | 2011-12-07 | 西北大学 | Hydrogen-oxidizing bacteria WMQ-7, and separation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102268391A (en) * | 2011-07-09 | 2011-12-07 | 西北大学 | Hydrogen-oxidizing bacteria WMQ-7, and separation method and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 李振高等: "《土壤与环境微生物研究法》", 30 September 2008, article "第五章样品采集和保存处理", pages: 71-78 * |
| 杜善周等: "丛枝菌根对神东煤矿区塌陷地的修复作用与生态效应", 《科技导报》, vol. 28, no. 07, 31 December 2010 (2010-12-31), pages 41 - 44 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103396980A (en) * | 2013-06-18 | 2013-11-20 | 中国矿业大学(北京) | Field collection method of arbuscular mycorrhizal spores by rapid gathering |
| CN103396980B (en) * | 2013-06-18 | 2015-08-12 | 中国矿业大学(北京) | A kind of field collection method of fast enriching arbuscular mycorrhiza spore |
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