CN102757511B - A kind of Grifola frondosa galactan and preparation method thereof - Google Patents
A kind of Grifola frondosa galactan and preparation method thereof Download PDFInfo
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- CN102757511B CN102757511B CN201210201951.6A CN201210201951A CN102757511B CN 102757511 B CN102757511 B CN 102757511B CN 201210201951 A CN201210201951 A CN 201210201951A CN 102757511 B CN102757511 B CN 102757511B
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Abstract
The invention provides a kind of Grifola frondosa galactan and preparation method thereof.The Grifola frondosa galactan of the present invention is to be obtained by hot water extraction, ultrafiltration, precipitate with ethanol, ion column chromatography, ultrafiltration again, lyophilization.The Grifola frondosa galactan of the present invention, purity reaches more than 90%, and molecular weight is daltonian more than 90% more than 5,900,000, has certain growth inhibited effect to tumor.
Description
Technical field
The present invention relates to edible and medical fungi field, relate in particular to a kind of Grifola frondosa galactan and preparation method thereof.
Background technology
Grifola frondosa is as a kind of edible and medical fungi, and its bioactive ingredients is mainly polysaccharose substance.At present, both at home and abroad to it
Separation Research achievement shows, the active component of Grifola frondosa is mainly 3 glucosans, albumen glucosan, heteropolysaccharide etc., and molecular weight exists
Within 2000000 dalton, there is immunocompetence and suppression tumor promotion.But, there are no separation acquisition both at home and abroad and there is biology
The research report of the galactan of activity.
Summary of the invention:
Present invention firstly provides a kind of Grifola frondosa galactan, its preparation principle is: first with hot water extraction ash tree beggar
Entity, integrated use high molecular thereafter (more than 100KD) membrane ultrafiltration, low concentration precipitate with ethanol (less than 30%), ion-exchange chromatography skill
Art separation Aqueous extracts, purification obtains the Grifola frondosa galactan solution of high molecular (more than 5900KD), concentrated, dry (cold
Lyophilizing is dry) obtain Grifola frondosa galactan.
Specifically, a kind of Grifola frondosa galactan of the present invention, it is prepared via a method which to obtain:
1. the water logging of 10～50 times of volumes there is not Grifola Frondosa sporophore, and 80～100 DEG C extract 1～5 hour；
2. lixiviating solution carries out ultrafiltration by the daltonian hollow fiber ultrafiltration membrane in 10～1,000,000 the most afterwards, and collection is trapped
Part, i.e. trapped fluid；
3. trapped fluid addition 10～the ethanol water of 50%, then collect precipitation；
4. precipitate through distilled water dissolve after, with～50% sodium-chloride water solution carry out ion-exchange chromatography；
5. first salt peak part daltonian hollow fiber ultrafiltration membrane in 10～1,000,000 of ion-exchange chromatography surpasses
Filter, collects the solution being trapped；
6. by above-mentioned solution concentrate drying, Grifola frondosa galactan is i.e. obtained.
The preferably Grifola frondosa galactan of the present invention is prepared via a method which to obtain:
1. the water logging of 10～30 times of volumes there is not Grifola Frondosa sporophore, and 90～100 DEG C extract 1～3 hour；
2. leaching body fluid carries out ultrafiltration by the daltonian hollow fiber ultrafiltration membrane in 50～1,000,000 the most afterwards, and collection is trapped
Part, i.e. trapped fluid；
3. trapped fluid addition 10～the ethanol water of 30%, then collect precipitation；
4. precipitate through distilled water dissolve after, with～30% sodium-chloride water solution carry out ion-exchange chromatography；
5. first salt peak part daltonian hollow fiber ultrafiltration membrane in 50～1,000,000 of ion-exchange chromatography surpasses
Filter, collects the solution being trapped；
6. by above-mentioned solution concentrate drying, Grifola frondosa galactan is i.e. obtained.
Present invention also offers the method preparing above-mentioned Grifola frondosa galactan, the method comprises the steps:
2. 80～100 DEG C of floodings；
2. the lixiviating solution daltonian hollow fiber ultrafiltration membrane in 10～1,000,000 carries out ultrafiltration；
3. the ultrafiltrate ethanol water of 10～50% precipitates；
4., after precipitation water dissolution, ion-exchange chromatography is carried out；
5. first salt peak part daltonian hollow fiber ultrafiltration membrane in 10～1,000,000 of ion-exchange chromatography surpasses
Filter；
6. ultrafiltrate concentrate drying i.e. obtains Grifola frondosa galactan.
Present invention also offers the application in the medicine of preparation prevention or treatment tumor of the above-mentioned Grifola frondosa galactan.On
State Grifola frondosa galactan sea and may be used for prevention or treatment tumor.
It is an advantage of the current invention that:
1. united application water carries, ultrafiltration, precipitate with ethanol and ion column chromatograph, and separates first and obtains high molecular (molecular weight is more than
5900000 dalton, high effective liquid chromatography for measuring) galactan；
3. step easily operates, the shortest, purity high (galactan content more than 90%, high effective liquid chromatography for measuring)；
4. the galactan obtained has the effect improving organism immune activity, suppressing tumor, can be used for preparation anti-swollen
Tumor, improve immunocompetent preparation.
Detailed description of the invention
Material source:
Grifola Frondosa sporophore: be purchased from Zhejiang Qingyuan's grid pharmaceutcal corporation, Ltd
Ultrafilter membrane: hollow-fibre membrane (molecular weight that dams be respectively 10,000,100,000,500,000,750,000,1,000,000 etc.), purchased from upper
Hai Fulite Industrial Co., Ltd.
The pillar of ion column chromatography: gel column (26 × 100cm), filler is Sephacryl S-200, S300, S400HR
Series (purchased from Amersham company)
Embodiment one:
The water logging using 10 times there is not Grifola Frondosa sporophore 1kg, under the conditions of 80 DEG C, is incubated 2 hours, is repeated 4 times, extracting solution
After 5000rpm/min is centrifugal, carries out ultrafiltration with the ultrafilter membrane of 100,000, collects the part being trapped；Retain by 10% ethanol precipitation
Liquid, after precipitate dissolves with distilled water, through ion column chromatography (flowing is 50% sodium chloride mutually), collects first main peak of salt phase
Part, then through 1,000,000 ultrafilter membrane ultrafiltration desalination, concentration, last lyophilization i.e. obtains about Grifola frondosa galactan 10g,
By Syrups by HPLC, its middle-molecular-weihydroxyethyl Grifola frondosa galactan more than 5,900,000 is more than 90%.
Embodiment two:
The water logging using 20 times there is not Grifola Frondosa sporophore 1kg, under the conditions of 90 DEG C, is incubated 1 hour, is repeated 3 times, extracting solution
After 5000rpm/min is centrifugal, carries out ultrafiltration with the ultrafilter membrane of 500,000, collects the part being trapped；Retain by 20% ethanol precipitation
Liquid, after precipitate dissolves with distilled water, through ion column chromatography (flowing is 30% sodium chloride mutually), collects first main peak of salt phase
Part, then through 750,000 ultrafilter membrane ultrafiltration desalination, concentration, last lyophilization i.e. obtains about Grifola frondosa galactan 15g, its
Middle-molecular-weihydroxyethyl Grifola frondosa galactan more than 5,900,000 is more than 90%.
Embodiment three:
The water logging using 40 times there is not Grifola Frondosa sporophore, under the conditions of 100 DEG C, is incubated 1 hour, is repeated 2 times, extracting solution warp
Carry out ultrafiltration with the ultrafilter membrane of 750,000 after 5000rpm/min is centrifugal, collect the part being trapped；Trapped fluid is precipitated with 30% ethanol,
After precipitate dissolves with distilled water, through ion column chromatography (flowing is 10% sodium chloride mutually), collect first main peak portion of salt phase
Point, then through 500,000 ultrafilter membrane ultrafiltration desalination, concentration, last lyophilization i.e. obtains about Grifola frondosa galactan 15g, wherein
Molecular weight Grifola frondosa galactan more than 5,900,000 is more than 90%.
Embodiment 4 tumor suppression active animal is tested:
1 given the test agent
The Grifola frondosa galactan that embodiment 1 prepares, with normal saline to desired concn, intraperitoneal administration volume
For 0.2ml/20g Mus.With cyclophosphamide as control sample, Hengrui Medicine Co., Ltd., Jiangsu Prov.'s (lot number: 11031921), take
Injection is with normal saline dilution to desired concn (3mg/ml).
2 laboratory animals
ICR mice (SPF level), Shanghai Si Laike laboratory animal responsibility company limited, the animal quality certification number: SCXK(Shanghai)
2007-0005；The animal quality quality certification number: 2007000530457.Body weight: 18-20g/6 week old.
3 tumor sources: S180 solid tumor is passed on maintenance by Shanghai Institute of Pharmaceutical Industry's Pharmacological Evaluation research center.
4 test methods
Extract appropriate seroperitoneum with aseptic empty needle, press 1:8 with 0.9% sodium chloride solution of sterilizing and dilute, in 0.5h every
Right side of mice axillary fossa subcutaneous vaccination tumor cell suspension 0.2ml.Inoculation time random packet in the future is administered, after last is administered, by little
Mus cervical dislocation is put to death, and dissects rapidly taking-up tumor mass and weighs, by following equation calculating tumor control rate:
Tumor control rate %=［ (matched group average tumor weight-administration group average tumor weight)/matched group average tumor weight ］ × 100%
Spleen index=administration group average spleen weight (g)/administration group average weight (10g)
Thymus index=average chest gland weight (g) of administration group/administration group average weight (10g)
Packet situation:
1) Grifola frondosa galactan (1mg/kg, ip × 10qd)
2) Grifola frondosa galactan (5mg/kg, ip × 10qd)
3) Grifola frondosa galactan (25mg/kg, ip × 10qd)
4) cyclophosphamide (30mg/kg, ip × 7qd)
5) comparison (ip × 10qd)
5 experiment conclusion
The each dosage of Grifola frondosa galactan has necessarily according to ip × 10qd administration, the growth to mice S180 solid tumor
Inhibitory action, immune organ is had facilitation.Experimental result and related data refer to table 1.
Mice S180 solid tumor models antitumor curative effect is tested quantitative dose-effect relationship by table 1 Grifola frondosa galactan
Compare with matched group: * P < 0.05, * * P < 0.01.
Claims (3)
1. a Grifola frondosa galactan, it is characterised in that it is prepared via a method which to obtain:
1. the water logging of 10～50 times of volumes there is not Grifola Frondosa sporophore, and 80～100 DEG C extract 1～5 hour；
2. lixiviating solution carries out ultrafiltration by the daltonian hollow fiber ultrafiltration membrane in 10～1,000,000 the most afterwards, collects the portion being trapped
Point, i.e. trapped fluid；
3. trapped fluid addition 10～the ethanol water of 50%, then collect precipitation；
4. precipitating after distilled water dissolves, the sodium-chloride water solution with less than 50% carries out ion-exchange chromatography；
5. first salt peak part daltonian hollow fiber ultrafiltration membrane in 10～1,000,000 of ion-exchange chromatography carries out ultrafiltration,
Collect the solution being trapped；
6. by above-mentioned solution concentrate drying, Grifola frondosa galactan is i.e. obtained.
Grifola frondosa galactan the most according to claim 1, it is characterised in that it is prepared via a method which to obtain:
1. the water logging of 10～30 times of volumes there is not Grifola Frondosa sporophore, and 90～100 DEG C extract 1～3 hour；
2. leaching body fluid carries out ultrafiltration by the daltonian hollow fiber ultrafiltration membrane in 50～1,000,000 the most afterwards, collects the portion being trapped
Point, i.e. trapped fluid；
3. trapped fluid addition 10～the ethanol water of 30%, then collect precipitation；
4. precipitating after distilled water dissolves, the sodium-chloride water solution with less than 30% carries out ion-exchange chromatography；
5. first salt peak part daltonian hollow fiber ultrafiltration membrane in 50～1,000,000 of ion-exchange chromatography carries out ultrafiltration,
Collect the solution being trapped；
6. by above-mentioned solution concentrate drying, Grifola frondosa galactan is i.e. obtained.
3. the application in the medicine of preparation prevention or treatment tumor of the Grifola frondosa galactan described in any one of claim 1 or 2.
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| CN201210201951.6A CN102757511B (en) | 2012-06-17 | 2012-06-17 | A kind of Grifola frondosa galactan and preparation method thereof |
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| CN102757511B true CN102757511B (en) | 2016-12-21 |
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| CN105754005A (en) * | 2016-03-04 | 2016-07-13 | 江苏江大源生态生物科技股份有限公司 | Method for extracting grifola frondosa polysaccharase with high yield |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1634983A (en) * | 2004-10-20 | 2005-07-06 | 上海市农业科学院 | Method for preparing Griflola frondosa proteoglycan |
| CN101659706A (en) * | 2008-08-25 | 2010-03-03 | 徐财泉 | Method for purifying and separating X-fraction polysaccharide from fruit bodies or mycelia of polyporus frondosus |
| CN101736053A (en) * | 2010-02-01 | 2010-06-16 | 南京泽朗医药科技有限公司 | Technique for extracting Grifola frondosa water-soluble polysaccharide |
| CN102408494A (en) * | 2011-11-30 | 2012-04-11 | 杭州众芝康菇生物技术有限公司 | Grifola frondosa polysaccharide ZZK component and preparation method thereof |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1634983A (en) * | 2004-10-20 | 2005-07-06 | 上海市农业科学院 | Method for preparing Griflola frondosa proteoglycan |
| CN101659706A (en) * | 2008-08-25 | 2010-03-03 | 徐财泉 | Method for purifying and separating X-fraction polysaccharide from fruit bodies or mycelia of polyporus frondosus |
| CN101736053A (en) * | 2010-02-01 | 2010-06-16 | 南京泽朗医药科技有限公司 | Technique for extracting Grifola frondosa water-soluble polysaccharide |
| CN102408494A (en) * | 2011-11-30 | 2012-04-11 | 杭州众芝康菇生物技术有限公司 | Grifola frondosa polysaccharide ZZK component and preparation method thereof |
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Granted publication date: 20161221 |