CN102754892B - Fresh-keeping synergist for fresh marine functional spirulina - Google Patents
Fresh-keeping synergist for fresh marine functional spirulina Download PDFInfo
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Abstract
The invention discloses a fresh-keeping synergist for fresh marine functional spirulina. 100ml of fresh-keeping synergist is prepared by mixing 0.5-3g of marine sulfated galactooligosaccharides (oligomerization carrageenan), 0.5-5g of isomaltose hypgather, 0.1-1g of citric acid, 0.1-1g of Vitamin C, one or multiple of 0.1-0.3g of carboxymethylcellulose, 0.1-0.4g of agar, 0.1-0.3g of sodium hyaluronate and 0.1-0.3g of bacterial cellulose, and the balance of purified water. A specific preparation method comprises the steps of: adding the marine sulfated galactooligosaccharides, the isomaltose hypgather, the citric acid, and one or multiple of the carboxymethylcellulose, the agar, the sodium hyaluronate and the bacterial cellulose into the purified water according to quantity in a formula, then heating for boiling for 20 minutes, standing for cooling, adding the Vitamin C, and mixing to reach a uniform state to obtain the fresh-keeping synergist. When in use, the fresh-keeping synergist provided by the invention is mixed with the fresh spirulina according to the proportion of (1:10)-(3:10). The fresh-keeping synergist not only plays a role in keeping freshness for the cultivated fresh spirulina, but also has the functions of sterilizing, resisting to virus, oxidation and tumor, enhancing immunity, reducing blood sugar and blood fat, promoting growth of intestinal probiotics, improving cardio-cerebral vessels and the like, so that the effect of increasing the spirulina is enhanced.
Description
Technical field
The present invention relates to a kind of fresh-keeping synergist, specifically a kind of fresh-keeping synergist for fresh and alive oceanic functional spirulina.
Background technology
Spirullina contains abundant good protein, multivitamin, mineral matter and multiple bioactivator in water plant, and its cell membrane is made of polysaccharide, is absorbed by human consumption easily, can be rated as best environmental protective hygienic food.Now spirulina on the market is the tablet made through oven dry processing, capsule, pulvis etc. basically, and fresh and alive spirulina product is arranged seldom, from basic general knowledge, be rich in the fruit or vegetable type food of chlorophyll and vitamin as can be seen, fresh certain for safer, more nutrition dry or that be processed into tin product, and fish, the shrimp of being rich in protein also must be fresh and alive better than the goods of reprocessing after the death.Scientific experiment proves, spirulina because of its cell membrane as thin as a wafer and nutritious, adopt industrialization processing method in the past, very easily make the spirulina quality impaired, the chlorophyll of dry product spirulina and amino acid whose content reduce more than 20% than bright product, content is up to 3750 international units in the bright product of polyphenoils mutase " SOD ", then all deactivations in dried algae, rotten or degradation phenomena in various degree also takes place in the other biological active material, and the stench flavor in the spirulina dry product is exactly the external reflection of spirulina qualitative change.Therefore, a kind of preparation of spirulina long-period freshness preserving and synergy that can make of invention has very big social benefit.
Summary of the invention
The invention provides a kind of fresh-keeping synergist for fresh and alive oceanic functional spirulina, can reach the effect that makes the fresh-keeping synergy of spirulina.
The present invention adopts technical scheme to be: adopt ocean sulfuric acid galactooligosacchari(es (oligomeric carragheen) 0.5g～3g, oligoisomaltose 0.5g～5g, citric acid 0.1g～1g, vitamin C 0.1g～1g, add purified water with wherein one or more of carboxymethyl cellulose 0.1g～0.3g, agar 0.1g～0.4g, Sodium Hyaluronate 0.1g～0.3g, bacteria cellulose 0.1g～0.3g and be mixed and made into fresh-keeping synergist 100ml to 100ml.Concrete preparation method: with wherein one or more of ocean sulfuric acid galactooligosacchari(es, oligoisomaltose, citric acid and sodium carboxymethylcellulose, agar, Sodium Hyaluronate, bacteria cellulose according in the formula ratio adding purified water, heating was boiled 20 minutes then, put cold back adding vitamin C and mix, namely.Fresh and alive spirulina mixes according to 1: 10～3: 10 ratio with preparation of the present invention during use.
Beneficial effect of the present invention: ocean sulfuric acid galactooligosacchari(es has antibiotic, anti-freezing, antiviral, anti-oxidant, antitumor and strengthen the effect of immunity in the preparation of the present invention, oligoisomaltose can the adjustable screw algae taste, be again the important prebiotics of probio simultaneously, the title of Oilgosaccharkdes is arranged; Citric acid has the taste of adjusting and corrosion-resistant effect; Vitamin C can be regulated taste, anti-oxidant; Carboxymethyl cellulose, agar, Sodium Hyaluronate, bacteria cellulose play the effect of suspension to spirulina, can form layer protecting film on the surface of spirulina simultaneously, make spirulina be difficult for corrupt the decomposition, and play preservation.In sum, the present invention not only can play fresh-keeping effect to culturing the fresh and alive spirulina that comes out, itself also have simultaneously antibiotic, antiviral, anti-oxidant, antitumor, strengthen immunity, hypoglycemic, impel beneficial bacteria of intestinal tract to grow, improve functions such as cardiovascular and cerebrovascular, thereby increase the efficacy effect of spirulina.
Compound basis
Ocean sulfuric acid galactooligosacchari(es has anti-freezing, multiple biologically active such as antibiotic, antiviral, anti-oxidant and antitumor.Ocean sulfuric acid galactooligosacchari(es has anticoagulating active preferably, and with respect to other anti-coagulants less side effect is arranged.Ocean sulfuric acid galactooligosacchari(es has very strong antiviral activity, has many report oligosaccharides that HIV is had the good restraining effect in recent years.Just the someone found that heparin has inhibitory action to simple scar exanthema virus HSV as far back as 1964, so often be starting point with the quasi-heparin substance in research process, antiviral activity to various natural and manually modified sulfated polysaccharides is inquired into, discover in antiviral process a certain section oligosaccharides that ad hoc structure arranged playing a part crucial.Ocean sulfuric acid galactooligosacchari(es has the activity of good enhancing body's immunity, all can play good activation and facilitation to nospecific immunity and specific immunity system.Ocean sulfuric acid galactooligosacchari(es can with macrophage on receptors bind, activate the latter, make it discharge IL-1, cause T-cell surface IL-2 expression of receptor simultaneously, accelerate the T cell maturation and discharged IL-2, after IL-2 and the receptors bind, further having accelerated T cell differentiation and maturation is cytotoxic T cell, thereby produces antitumor action by this synergy each other.
Oligoisomaltose flavor matter is fine, can be used to instead of part sucrose, improves the food mouthfeel, reduces sugariness.Simultaneously oligoisomaltose can effectively promote the growth and breeding of beneficial bacteria-Bifidobacterium in the human body, thus be called " positive growth factor for bifidus " again, enter human body after, can promote the growth and breeding of Bifidobacterium in the enteron aisle effectively.Can suppress simultaneously the spoilage organisms growth, but for a long time edible delaying senility, defaecation, antibacterial, anti-cancer, anticancer, alleviate burden of liver, improve the nutrient absorption rate.
Citric acid has gentle frank tart flavour, generally is used for the manufacturing of food such as various beverages, carbonated drink, grape wine, candy, dessert, biscuit, canned fruit juice, dairy products.Improve the sensory properties of food simultaneously, whet the appetite and promote digesting and assimilating of calcium in the body, phosphorus substance.Ester class such as the triethyl citrate of citric acid can be made nontoxic plasticizer, make the used for packing foods plastic sheeting, are the acids of drink and food industry, anticorrisive agent.
Vitamin C is the L-ascorbic acid again, is a kind of water soluble vitamin.Vitamin C has effect anti-oxidant and that improve body immunity.The injury that ascorbic antioxidation can be resisted the radical pair cell prevents the variation of cell; Simultaneously can also protect other antioxidant, as vitamin A, vitamin E, unrighted acid, prevent the injury of radical pair human body.Vitamin C can strengthen chemotaxis and the deformability of neutrophil leucocyte, improves sterilizing ability, also can promote lymphoblastic generation, improves body to the identification of external and malignant cell and kills.
Carboxymethyl cellulose can form full-bodied colloid, solution, adhesion is arranged, thickening, flow, emulsification dispersion, figuration, water conservation, protecting colloid, film forming, acidproof, salt tolerant, outstanding characteristic such as turbid; and physiology is harmless, therefore is used widely in fields such as food, medicine, daily use chemicals, oil, papermaking, weaving, building produce.
Agar be by agar or river hedge (belonging to red algae) through be heated to dissolve after, the marine alga elite that forms of cooled and solidified in addition.Agar contains rich dietary fiber, the protein content height, and heat is low, has toxin-expelling and face nourishing, purging intense heat, ease constipation, hypotensive, hypoglycemic and protective effect on cancer risk.
Sodium Hyaluronate demonstrates multiple important physical function with its distinctive molecular structure and physicochemical property in body, as lubricated joint, regulate the permeability of vascular wall, regulates protein, and Water-Electrolyte diffusion and running promote wound healing etc.And has special water retention.
Bacteria cellulose has very strong hydrophily, vicidity and stability, can be used as food forming agent, thickener, dispersant, anti-solvation, improve mouthfeel as the skeleton of casing and some food, become a kind of novel important foodstuff base material and dietary fiber.
The function of this invention preparation
1, spirulina there is the effect of fresh-keeping synergy
Preparation of the present invention has excellent antibiotic, antiviral, oxidation resistant effect, can make spirulina keep fresh activity, can increase the efficacy effect of spirulina simultaneously.
2, adjustable screw algae taste, improve micro-ecological environment in the human body
The compound sugar that uses in the said preparation not only can the adjustable screw algae taste, reduce the stench flavor of spirulina, and compound sugar can promote the growth and breeding of Bifidobacterium in the enteron aisle effectively, suppress the spoilage organisms growth, but for a long time edible delaying senility, defaecation, antibacterial, anti-cancer, anticancer, alleviate burden of liver, improve the nutrient absorption rate.
3, hypoglycemic, enhancing immunologic function
Contained active material in the said preparation can reduce the content of blood sugar for human body blood fat, activates and promote nospecific immunity and specific immunity system to strengthen the body's immunity activity.
The specific embodiment
Embodiment 1: the fresh-keeping synergist of a kind of fresh and alive oceanic functional spirulina, it adopts ocean sulfuric acid galactooligosacchari(es 2g, oligoisomaltose 3g, citric acid 0.5g, vitamin C 0.5g, carboxymethyl cellulose 0.1g, agar 0.1g, purified water 100ml to be mixed and made into fresh-keeping synergist 100ml.The purified excellent species that filters out of spirulina carries out Continuous Cultivation, propagation then in free of contamination industrial aquaculture device.The spirulina that cultures through centrifugal, collect the back and wash 2～3 times with 1.5% sodium acid carbonate, use the axenic purification water washing again 2～3 times, weighing then becomes oral liquid 10ml/ bottle according to the mixing of 1: 10 ratio of spirulina and fresh-keeping synergist, can.Through 6 months observation, this spirulina did not have rotten, corruption.
Embodiment 2: the fresh-keeping synergist of a kind of fresh and alive oceanic functional spirulina, it adopts ocean sulfuric acid galactooligosacchari(es 1g, oligoisomaltose 4g, citric acid 0.2g, vitamin C 1g, carboxymethyl cellulose 0.1g, Sodium Hyaluronate 0.1g, purified water 100ml to be mixed and made into fresh-keeping synergist 100ml.The purified excellent species that filters out of spirulina carries out Continuous Cultivation, propagation then in free of contamination industrial aquaculture device.The spirulina that cultures through centrifugal, collect the back and wash 2～3 times with 1.5% sodium acid carbonate, use the axenic purification water washing again 2～3 times, weighing then becomes oral liquid 10ml/ bottle according to the mixing of 1: 10 ratio of spirulina and fresh-keeping synergist, can.Through 6 months observation, this spirulina did not have rotten, corruption.
Embodiment 3: the fresh-keeping synergist of a kind of fresh and alive oceanic functional spirulina, it adopts ocean sulfuric acid galactooligosacchari(es 3g, oligoisomaltose 1g, citric acid 1g, vitamin C 0.3g, Sodium Hyaluronate 0.2g, bacteria cellulose 0.1g, purified water 100ml to be mixed and made into fresh-keeping synergist 100ml.The purified excellent species that filters out of spirulina carries out Continuous Cultivation, propagation then in free of contamination industrial aquaculture device.The spirulina that cultures through centrifugal, collect the back and wash 2～3 times with 1.5% sodium acid carbonate, use the axenic purification water washing again 2～3 times, weighing then becomes oral liquid 10ml/ bottle according to the mixing of 1: 10 ratio of spirulina and fresh-keeping synergist, can.Through 6 months observation, this spirulina did not have rotten, corruption.
Embodiment 4: the fresh-keeping synergist of a kind of fresh and alive oceanic functional spirulina, it adopts ocean sulfuric acid galactooligosacchari(es 2g, oligoisomaltose 3g, citric acid 0.5g, vitamin C 0.5g, agar 0.1g, bacteria cellulose 0.1g, purified water 100ml to be mixed and made into fresh-keeping synergist 100ml.The purified excellent species that filters out of spirulina carries out Continuous Cultivation, propagation then in free of contamination industrial aquaculture device.The spirulina that cultures through centrifugal, collect the back and wash 2～3 times with 1.5% sodium acid carbonate, use the axenic purification water washing again 2～3 times, weighing then becomes oral liquid 10ml/ bottle according to the mixing of 2: 10 ratio of spirulina and fresh-keeping synergist, can.Through 6 months observation, this spirulina did not have rotten, corruption.
Embodiment 5: the fresh-keeping synergist of a kind of fresh and alive oceanic functional spirulina, it adopts ocean sulfuric acid galactooligosacchari(es 2g, oligoisomaltose 5g, citric acid 0.2g, vitamin C 0.1g, carboxymethyl cellulose 0.1g, agar 0.1g, Sodium Hyaluronate 0.1g, purified water 100ml to be mixed and made into fresh-keeping synergist 100ml.The purified excellent species that filters out of spirulina carries out Continuous Cultivation, propagation then in free of contamination industrial aquaculture device.The spirulina that cultures through centrifugal, collect the back and wash 2～3 times with 1.5% sodium acid carbonate, use the axenic purification water washing again 2～3 times, weighing then becomes oral liquid 10ml/ bottle according to the mixing of 3: 10 ratio of spirulina and fresh-keeping synergist, can.Through 6 months observation, this spirulina did not have rotten, corruption.
Embodiment 6: human feeding trial 1
Use the fresh-keeping synergist of the present invention of preparation among the embodiment 1, the effect of this product is tested.
The study subject standard: the crowd of simple hypoimmunity, the age is between 18～65 years old.
Experimental design and grouping: adopt two kinds of control design between self and group.By above-mentioned Standard Selection 100 routine experimenters, divide into groups according to the requirement of randomized double-blind, be divided into test-meal group and control group, consider influence result's principal element such as age, sex, diet etc. as far as possible, carry out harmony to check, with the comparativity between the assurance group.Every group of experimenter's 50 examples.The test-meal group is taken the spirulina twice that adds fresh-keeping synergist for each person every day, and each 1 bottle, control group is taken pure spirulina, takes continuously 30 days.
The grouping situation sees Table 1.The preceding two groups of blood of test-meal, urine, just routine and hepatic and renal function, Chest X-rays, electrocardiogram, B ultrasonic etc. check all in normal range (NR), the equal no significant difference of content (P＞0.05) of two groups of interior macrophages of subject age, sex, blood medium size lymphocyte and body has comparativity.
Data relatively as table 1 test-meal was last
After the test-meal situation see Table 2, table 3.
Table 2 blood testing-lymphocyte
Table 3 body fluid chemical examination-macrophage
By table 2, table 3 as seen, took back 30 days, the content of lymphocyte and macrophage all obviously improves before and after the test-meal of test-meal group, and the content of lymphocyte and macrophage also increases before and after the control group test-meal, but less with respect to test-meal group increase rate.
The result shows, the examination clothes added the spirulina of preparation of the present invention after 30 days, self relatively reach comparison between test-meal group and control group group after the test-meal before and after the test-meal of test-meal group, the content of macrophage obviously increases (P＜0.01) in blood medium size lymphocyte and the body, so this invention preparation can improve the function that spirulina strengthens immunity.
Human feeding trial 2
Use the fresh-keeping synergist of the present invention of preparation among the embodiment 2, the effect of this product is tested.
The study subject standard: the crowd of simple dyslipidemia, keep usual diet, blood sampling is twice in half a year.Twice serum total cholesterol (TC) is 〉=5.2mmol/L or twice serum triglyceride (TG) 〉=1.65mmol/L.
Experimental design and grouping: adopt two kinds of control design between self and group.By above-mentioned Standard Selection 102 routine experimenters, divide into groups according to the requirement of randomized double-blind.Be divided into test-meal group and control group at random by experimenter's blood lipid level, consider influence result's principal element such as age, sex, diet etc. as far as possible, carry out harmony to check, with the comparativity between the assurance group.Every group of experimenter's 51 examples.The test-meal group is taken the spirulina 2 times that adds this invention preparation for each person every day, and each 1 bottle, control group is taken pure spirulina, takes continuously 30 days.
The result
Before the test-meal, test-meal group and control group crowd's age, mental status, sleep state, diet situation do not have significant difference, and two groups of crowds' fluoroscopy of chest, electrocardiogram and Abdominal B type ultrasonography check result show no obvious abnormalities.Serum TC, TG there are no significant difference (P＞0.05) sees Table 4.
The preceding two groups of crowd's serum TCs of table 4 test-meal, TG content are relatively
After the test-meal, test-meal group crowd's serum TC and TG content all obviously reduce, self relatively reach and more all have significant difference (P＜0.01 or P＜0.05) between the control group group, see Table 5-table 6, show that the purer spirulina of spirulina that adds this invention preparation has the effect of better reduction serum TC, TG content.
Two groups of crowds' serum TC content relatively before and after table 5 test-meal
Two groups of crowds' serum TG content relatively before and after table 6 test-meal
The crowd's of test-meal group as a result serum TC and TG content all obviously reduce, and self relatively reach more all to have significant difference (P＜0.01 or P＜0.05) between the control group group, show to add the function that this invention preparation can strengthen the reducing blood lipid of spirulina.
Human feeding trial 3
Use the fresh-keeping synergist of the present invention of preparation among the embodiment 3, the effect of this product is tested.
The study subject standard: the crowd of simple pathoglycemia, keep usual diet, blood sampling is twice in half a year.The fasting blood-glucose index all 〉=7.0mmol/L.
Experimental design and grouping: adopt two kinds of control design between self and group.By above-mentioned Standard Selection 100 routine experimenters, divide into groups according to the requirement of randomized double-blind.Be divided into test-meal group and control group at random by experimenter's fasting blood glucose level, consider influence result's principal element such as age, sex, diet etc. as far as possible, carry out harmony to check, with the comparativity between the assurance group.Every group of experimenter's 50 examples.The test-meal group is taken the spirulina 2 times that adds this invention preparation for each person every day, and each 1 bottle, control group is taken pure spirulina, takes continuously 30 days.
Before the test-meal, two groups of fasting blood-glucose index there was no significant differences (P＞0.05) see Table 7.
The preceding two groups of crowd's fasting blood-glucose content of table 7 test-meal relatively
After the test-meal, test-meal group crowd's fasting blood-glucose obviously reduces, self relatively reach and the control group group between more all have significant difference (P＜0.01).Details sees Table 8.
Two groups of crowd's fasting blood-glucose content relatively before and after table 8 test-meal
The crowd's of test-meal group as a result fasting blood-glucose content obviously reduces, self relatively reach and the control group group between more all have significant difference (P＜0.01 or P＜0.05), show to add the hypoglycemic function that this invention preparation can strengthen spirulina.
Claims (3)
1. fresh-keeping synergist of fresh and alive oceanic functional spirulina, adopt ocean sulfuric acid galactooligosacchari(es 0.5g～3g, oligoisomaltose 0.5g～5g, citric acid 0.1g～1g, vitamin C 0.1g～1g, add purified water with wherein one or more of carboxymethyl cellulose 0.1g～0.3g, agar 0.1g～0.4g, Sodium Hyaluronate 0.1g～0.3g, bacteria cellulose 0.1g～0.3g and be mixed and made into fresh-keeping synergist 100ml to 100ml.
2. the fresh-keeping synergist of a kind of fresh and alive oceanic functional spirulina according to claim 1, its preparation method is that wherein one or more of ocean sulfuric acid galactooligosacchari(es, oligoisomaltose, citric acid and sodium carboxymethylcellulose, agar, Sodium Hyaluronate, bacteria cellulose are added in the purified water according to formula ratio, heating was boiled 20 minutes then, put cold back adding vitamin C and mix, namely.
3. the fresh-keeping synergist of a kind of fresh and alive oceanic functional spirulina according to claim 1 mixes according to 1: 10～3: 10 ratio with fresh and alive spirulina during use.
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| CN105454976A (en) * | 2015-12-21 | 2016-04-06 | 南京农业大学 | Plant essential oil compound preservative and application thereof in preservation of living spirulina beverage |
| CN109497358A (en) * | 2018-10-17 | 2019-03-22 | 雷云飞 | A kind of active microalgae drinks and preparation method thereof |
| CN110393252A (en) * | 2019-08-22 | 2019-11-01 | 内蒙古春之潮食品有限责任公司 | A kind of processing method preventing the rancid smell of wheat-bran dietary fiber |
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