CN102742729A - Composite immunoenhancement feed additive for litopenaeus vannamei and application and feed thereof - Google Patents
Composite immunoenhancement feed additive for litopenaeus vannamei and application and feed thereof Download PDFInfo
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Abstract
The invention discloses a composite immunoenhancement feed additive for litopenaeus vannamei and an application and feed thereof. The composite immunoenhancement feed additive for litopenaeus vannamei comprises beta-dextran and selenium methionine; when selenium methionine is calculated on the basis of the selenium amount, the mass ratio of beta-dextran to selenium methionine is 150-300:0.2. According to the invention, experiments show that common basal feed for litopenaeus vannamei is added with the combination of beta-dextran and selenium methionine according to a certain mass ratio, or the combination of beta-dextran and vitamin E according to a certain mass ratio, or the combination of beta-dextran, selenium methionine and vitamin E according to a certain mass ratio; through the antioxidation approach of selenium methionine and vitamin E, the immune defense capability of litopenaeus vannamei bodies is improved; the immunostimulation time of beta-dextran is effectively prolonged; the using amount is decreased; and the immune fatigue problem generated when beta-dextran is used alone is solved. The additive of the invention can be added into basic ration of litopenaeus vannamei directly, and is convenient for use.
Description
Technical field:
The invention belongs to the aquatic feeds field, be specifically related to the feed that a kind of Environment of Litopenaeus vannamei Low strengthens feed addictive and application thereof with complex immunity and contains this feed addictive.
Background technology:
Environment of Litopenaeus vannamei Low (Litopenaeus vannamei) is commonly called as Penaeus Vannmei, is one of three the highest big kinds of world today's shrimp culture output; It is strong to have premunition; Fast growth, characteristics such as feeding habits are assorted, and nutritional requirement is low; Being the fine quality of " extra large shrimp is light to be supported ", also is Chinese cultured area and the highest breed variety of output.In recent years, along with the prawn culturing scale constantly enlarges, the improving constantly of cultivation density; Prawn culturing is faced with a large amount of problems; As the fierceness of the factors such as crowded, nutrition, environment, metabolism stress, cause the prawn anti-stress ability poor, a little less than the premunition; Very easily break out extensive disease, thereby cause enormous economic loss for the aquaculture of prawn.At present the disease prevention and cure about aquatic livestock mainly are to rely on antibiotic etc, but because the chemical sproof formation of pathogen, and the proposition of problem such as food security, correctly use antibiotic etc and basic disease prevention and cure countermeasure thereof to wait to establish.In addition, still do not have the efficacious therapy method at present, can only carry out such as generality such as the sterilization in breed pond and the cleaning of ovum are handled for the viral disease of prawn.Discover that beta glucan can improve the immunity and the premunition of shrimp body through the nospecific immunity that strengthens prawn as single immunopotentiator, is used widely aborning.But along with deepening continuously of research, exist the immunostimulation time short when finding in the feed single interpolation beta glucan, dosage is big, is prone to problems such as immune fatigue when using for a long time.
Summary of the invention:
First purpose of the present invention provides a kind of immunostimulation time that can effectively prolong beta glucan, reduces its using dosage, alleviates the Environment of Litopenaeus vannamei Low that produces immune fatigue problem when using beta glucan separately simultaneously and strengthens feed addictive with complex immunity.
Environment of Litopenaeus vannamei Low of the present invention strengthens feed addictive with complex immunity, it is characterized in that, comprises beta glucan and selenium methionine, and selenium methionine calculates with the amount of selenium, and beta glucan and selenium mass ratio are 150 ~ 300:0.2.
Described Environment of Litopenaeus vannamei Low strengthens feed addictive with complex immunity and further preferably also comprises vitamin E, and the mass ratio of beta glucan, selenium and vitamin E is: 150 ~ 300:0.2:100.
The present invention also provides other a kind of Environment of Litopenaeus vannamei Low to strengthen feed addictive with complex immunity, it is characterized in that, comprises beta glucan and vitamin E, and both mass ratioes are 150 ~ 300:100.
Second purpose of the present invention provides above-mentioned Environment of Litopenaeus vannamei Low and strengthens feed addictive in the application as the feed addictive of Environment of Litopenaeus vannamei Low with complex immunity.
The 3rd purpose of the present invention provides a kind of Environment of Litopenaeus vannamei Low and strengthens feed with complex immunity; It is characterized in that; Comprise that common Environment of Litopenaeus vannamei Low basal feed and Environment of Litopenaeus vannamei Low strengthen feed addictive with complex immunity; Described Environment of Litopenaeus vannamei Low strengthens feed addictive with complex immunity and comprises beta glucan and selenium methionine, and the addition of beta glucan is the common Environment of Litopenaeus vannamei Low basal feed of 150 ~ 300mg/kg, selenium methionine; Amount with selenium is calculated, and its addition is the common Environment of Litopenaeus vannamei Low basal feed of 0.2mg/kg.
Described Environment of Litopenaeus vannamei Low strengthens feed addictive with complex immunity and preferably also comprises vitamin E, and the addition of vitamin E is the common Environment of Litopenaeus vannamei Low basal feed of 100mg/kg.
Other a kind of Environment of Litopenaeus vannamei Low of the present invention strengthens feed with complex immunity; It is characterized in that; Comprise that common Environment of Litopenaeus vannamei Low basal feed and Environment of Litopenaeus vannamei Low strengthen feed addictive with complex immunity; Described Environment of Litopenaeus vannamei Low strengthens feed addictive with complex immunity and comprises beta glucan and vitamin E, and the addition of beta glucan is the common Environment of Litopenaeus vannamei Low basal feed of 150 ~ 300mg/kg, and the addition of vitamin E is the common Environment of Litopenaeus vannamei Low basal feed of 100mg/kg.
The present invention finds through experiment; Beta glucan and selenium methionine are united interpolation by certain mass ratio; Perhaps beta glucan and vitamin E are united interpolation by certain mass ratio; Perhaps beta glucan and selenium methionine, vitamin E are united by certain mass ratio and add in the common Environment of Litopenaeus vannamei Low basal feed; Improve the immune defense ability of Environment of Litopenaeus vannamei Low body through the anti-oxidant approach of selenium methionine and vitamin E, can effectively prolong beta glucan the immunostimulation time, reduce its using dosage, alleviate simultaneously and produce immune fatigue problem when using beta glucan separately.The present invention can directly add in the basal diet of Environment of Litopenaeus vannamei Low, and is easy to use.
Description of drawings:
Fig. 1 is the Environment of Litopenaeus vannamei Low hemolymph counting diagram of control feed and the feed that added the various combination additive of throwing something and feeding of throwing something and feeding.
Fig. 2 is the mensuration figure of the control feed and the serum phenol oxide enzymatic activity of the Environment of Litopenaeus vannamei Low of the feed that added the various combination additive of throwing something and feeding of throwing something and feeding.
Fig. 3 is throw something and feed control feed and the active mensuration figure of the serum lysozyme of the Environment of Litopenaeus vannamei Low of the feed that added the various combination additive of throwing something and feeding.
Fig. 4 is the white spot virus infection experiment mensuration figure of the control feed and the Environment of Litopenaeus vannamei Low of the feed that added the various combination additive of throwing something and feeding of throwing something and feeding.
Fig. 5 is the mensuration figure of the control feed and the serum TAC of the Environment of Litopenaeus vannamei Low of the feed that added the various combination additive of throwing something and feeding of throwing something and feeding.
Fig. 6 is the mensuration figure of the control feed and the Content of MDA of the Environment of Litopenaeus vannamei Low of the feed that added the various combination additive of throwing something and feeding of throwing something and feeding.
Fig. 7 is the mensuration figure of the control feed and the activity of SOD in serum of the Environment of Litopenaeus vannamei Low of the feed that added the various combination additive of throwing something and feeding of throwing something and feeding.
The specific embodiment:
Below be to further specify to of the present invention, rather than limitation of the present invention.
Embodiment 1:
One, feeding experiment
Feed divides into groups:
G0 organizes feed: common Environment of Litopenaeus vannamei Low basal feed
G1 organizes feed: in common Environment of Litopenaeus vannamei Low basal feed, add beta glucan, addition is every kilogram of common Environment of Litopenaeus vannamei Low basal feed 300mg beta glucan.
G2 organizes feed: in common Environment of Litopenaeus vannamei Low basal feed, add beta glucan and selenium methionine, addition is every kilogram of common Environment of Litopenaeus vannamei Low basal feed 300mg beta glucan, and 0.2mg selenium adds with the form of selenium methionine.
G3 organizes feed: in common Environment of Litopenaeus vannamei Low basal feed, add beta glucan and vitamin E, addition is every kilogram of common Environment of Litopenaeus vannamei Low basal feed 300mg beta glucan, the 100mg vitamin E.
G4 organizes feed: in common Environment of Litopenaeus vannamei Low basal feed, add beta glucan, selenium and vitamin E, addition is every kilogram of common Environment of Litopenaeus vannamei Low basal feed 300mg beta glucan, and 0.2mg selenium adds the 100mg vitamin E with the form of selenium methionine.
G5 organizes feed: in common Environment of Litopenaeus vannamei Low basal feed, add beta glucan, selenium and vitamin E, addition is every kilogram of common Environment of Litopenaeus vannamei Low basal feed 150mg beta glucan, and 0.2mg selenium adds the 100mg vitamin E with the form of selenium methionine.
Choose the Environment of Litopenaeus vannamei Low of the initial body weight of 960 tails for (0.83 ± 0.03) g; Be divided into 6 groups at random, every group of 4 repetitions, each repeats 40 tail shrimps; Routine is thrown something and fed respectively, and G0 organizes feed, G1 group feed, G2 group feed, G3 group feed, G4 organizes feed and G5 organizes feed, and the whole culture-cycle is 35 days.When feeding experiment finishes; Each cylinder is got 10 ~ 15 tail shrimps at random; Insert heart with the 1ml asepsis injector from the prawn head cuirass and extract hemolymph, merge and place aseptic Eppendorf pipe, leave standstill 3 ~ 4h under 4 ℃; Under 5 ℃,, draw the supernatant packing and be stored in-80 ℃ of refrigerators subsequent use with the centrifugal 10min of 10000r/min.
Two, effect detection:
1, prawn hemolymph counting
Haemocyte sum (THC) has reflected immunocompetence or the health status of prawn to a certain extent.In crustacean, THC has important role aspect the opposing cause of disease invasion, but because of extraneous changing factor, as infecting, and environment-stress, hormonal change etc. between ecdysis and bigger variation takes place.
When feeding experiment finished, every cylinder was got 5 tail shrimps at random, gets blood with the 1mL asepsis injector in cardiocoelom, merged to place aseptic Eppendorf pipe.Draw 150 μ L anti-coagulants in the Eppendorf pipe, add prawn blood 100 μ L immediately, mixing is got the blood of 50 μ L anti-freezings rapidly, adds the PBS buffer solution of 10mL pH 7.3, with cell counter (Z
2Coulter, BECKMAN COULTER) count.
Result of the test is with mean+SD (means ± SD) expression.Adopt SPSS13.0 software to carry out data statistics and analysis, earlier data are carried out one-way analysis of variance (One-wayANOVA), if significant difference remakes Duncan ' s multiple ratio, P＜0.05 expression otherness is remarkable.The result is as shown in Figure 1, and as can be seen from Figure 1, the hemolymph counting of G4, G5 group is significantly higher than G0 group (P < 0.05), organizes a little more than G1.
This shows that the additive that is constituted jointly by beta glucan and selenium methionine, vitamin E of the present invention has improved the immunocompetence of prawn.
2, the active mensuration of Environment of Litopenaeus vannamei Low serum phenol oxide enzyme (PO)
Phenol oxide enzyme (PO) is the copper-containing metal enzyme that has important function in the prawn nospecific immunity factor; Can produce melanin through enzymatic and non-enzymatic reaction; Formed melanin and metabolic intermediate thereof can suppress the activity of pathogen extracellular protein and chitinase; Thereby kill the exotic disease substance, play immanoprotection action.
Measuring principle: phenol oxide enzyme (PO) vigor is that the product with itself and substrate in the unit interval causes that the variation of absorbance confirms.
This test serum sample is stored in-80 ℃ of Environment of Litopenaeus vannamei Low blood serum samples in the refrigerator among the embodiment 1.
With the L-DOPA is substrate; Add 10uL serum in the 96 hole ELISA Plates, in each hole, adding 200 μ L concentration then is 0.1mol/L, and PH is 6.0 phosphate buffer; In each sample well, adding 10 μ L concentration at last is the L-DOPA liquid (available from Sigma company) of 0.01mol/L; (550, Bio-Rad) middle concussion is 4 times, and every separated 4min reads the light absorption value at 490nm place at ELIASA.Enzyme activity is with under the experiment condition, and it is an enzyme activity unit that OD490 value per minute increases by 0.001.
Result of the test is with mean+SD (means ± SD) expression.Adopt SPSS13.0 software to carry out data statistics and analysis, earlier data are carried out one-way analysis of variance (One-wayANOVA), if significant difference remakes Duncan ' s multiple ratio, P＜0.05 expression otherness is remarkable.The result is as shown in Figure 2, and as can beappreciated from fig. 2, the blood-serum P O activity of G4 group is significantly higher than the G0 group.
This shows that the additive that is constituted jointly by beta glucan and selenium methionine, vitamin E of the present invention can play better immanoprotection action to Environment of Litopenaeus vannamei Low.
3, the active mensuration of Environment of Litopenaeus vannamei Low serum lysozyme (LZM)
Lysozyme finds that in nineteen twenty-two it is a kind of effective antiseptic by Fu Laiming, and full name is 1, and 4-β-N-lysozyme is called mucopeptide N-acetyl group muramyl hydrolase or muramidase again.The sterilization mechanism of lysozyme is its mucopeptide layer that acts on bacteria cell wall, and mucopeptide is the cell membrane main component of bacterium.Lysozyme can cut off N-acetylglucosamine and β-1,4 glycosidic bond between the-acetylmuramic acid in the mucopeptide structure, destroys the mucopeptide support, and cell membrane is destroyed.Because one of critical function of bacteria cell wall is the protection bacterium, promptly anti-hypotonic, so after the protective effect of bacterium lost cell wall, in hypotonic environment, can dissolve.The main effective object of lysozyme is a gram-positive bacteria.
Measuring principle: lysozyme can make the dissolving of gram-positive bacteria cell wall, and is the most responsive like micrococcus lysodeikticus especially to saprophytic bacteria, so be index with the dissolving micrococcus lysodeikticus often, the activity of lysozyme value measured.
This test serum sample is stored in-80 ℃ of Environment of Litopenaeus vannamei Low blood serum samples in the refrigerator among the embodiment 1.
With the micrococcus lysodeikticus freeze-dried powder is substrate, and using the 0.1mol/LPH value is that 6.4 potassium phosphate cushioning liquid is made into substrate suspension (OD570=0.3).Get this suspension of 3ml in vitro, put in the ice bath, add 50ul serum mixing again, survey its initial light density value A0 value in the 570nm place, test solution is moved in 37 ℃ of water-baths be incubated 30min then, place ice bath 10min cessation reaction after the taking-up at once, survey its A value.Antalzyme activity (LZM) is calculated as follows: U=(A0-A)/A.
Result of the test is with mean+SD (means ± SD) expression.Adopt SPSS13.0 software to carry out data statistics and analysis, earlier data are carried out one-way analysis of variance (One-wayANOVA), if significant difference remakes Duncan ' s multiple ratio, P＜0.05 expression otherness is remarkable.The result is as shown in Figure 3, and as can beappreciated from fig. 3, the serum LSZ activity of G2, G3, G4 group is significantly higher than G0 group and G1 group.
This shows; The additive that constitutes jointly by beta glucan and selenium methionine of the present invention; Or the additive that constitutes jointly by beta glucan and vitamin E; Or the additive that is constituted jointly by beta glucan and selenium methionine, vitamin E three can improve the immune defense function of prawn, the immune fatigue problem when the alleviation glucan uses separately.
4, Environment of Litopenaeus vannamei Low white spot virus (WSSV) infection experiment is measured
Virus crude extract preparation: get about the sick shrimp muscle 0.3g that has the white spot virus infection; Shred mixing; Adding 1.0mLPH by 0.1g tissue is 0.01mol/L phosphate buffer mixing in homogenizer of 7.4, and homogenate is got supernatant with the centrifugal 10min of 10000r/min; Through 0.45 μ L filtering with microporous membrane, its filtrating is the WSSV crude extract.Crude extract prepares in 2h and finishes.Crude extract adds phosphate buffer and dilutes 5 times as parenteral solution, is used for the experiment of prawn infectable infection.
Infection experiment: do preliminary experiment before the experiment, touch to such an extent that the righttest injection volume is 50 μ L.From each repetition of embodiment 1, get 10 tail prawns and carry out infection experiment.Every endnote is penetrated WSSV crude extract 50 μ L, and each repeats to continue the original test feed of throwing something and feeding, and writes down cumulative mortality and the relative immunity protective rate of prawn in 3 days.Relative immunity protective rate=100 * (control group cumulative mortality-experimental group cumulative mortality)/control group cumulative mortality.
Result of the test is with mean+SD (means ± SD) expression.Adopt SPSS13.0 software to carry out data statistics and analysis, earlier data are carried out one-way analysis of variance (One-wayANOVA), if significant difference remakes Duncan ' s multiple ratio, P＜0.05 expression otherness is remarkable.The result is as shown in Figure 4, and as can beappreciated from fig. 4, the cumulative mortality of G2, G4, G5 group significantly is lower than G0 group (P＜0.05), organizes a little less than G1.
This shows; The additive that constitutes jointly by beta glucan and selenium methionine of the present invention; Or the additive that is constituted jointly by beta glucan and selenium methionine, vitamin E three can improve the immune defense function of prawn, the immune fatigue problem when the alleviation beta glucan uses separately.
5, the mensuration of Environment of Litopenaeus vannamei Low serum TAC (T-AOC)
The power and the body health degree of the TAC of body defense system (T-AOC) exist close ties, are the overall objectives that antioxidant ability of organism is estimated.This defense system has enzymatic and non-enzymatic two individual system; Many enzymes are to be the activated centre with the trace element; For example: superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), catalase (CAT), glutathione S-transferase (GST) etc. are mainly vitamin, amino acid and metalloprotein in the non-enzymatic reaction system.For example: VE, carrotene, VC, cysteine, methionine, tryptophan, histidine, glucose, CER, transferrins, lactoferrin etc.The protection oxidation of this individual system is mainly passed through elimination free radical and active oxygen in order to avoid cause lipid peroxidation; And decompose hydroperoxide, blocking-up peroxidating chain; Removed the metal ion of catalytic action simultaneously.
Measuring principle: many polyphenoils are arranged in the body, can make Fe
3+Be reduced into Fe
2+, the latter can form firm complex compound with luxuriant and rich with fragrance quinoline class material, can measure the height of its oxidation resistance through colorimetric.
This test serum sample is stored in-80 ℃ of Environment of Litopenaeus vannamei Low blood serum samples in the refrigerator among the embodiment 1.
TAC that this test is adopted (T-AOC) mensuration kit builds up bio-engineering research institute available from Nanjing, and (title: TAC (T-AOC) testing cassete (/ 50 kinds of 100 pipes) article No.: A015), the determination step by specification carries out.
Result of the test is with mean+SD (means ± SD) expression.Adopt SPSS13.0 software to carry out data statistics and analysis, earlier data are carried out one-way analysis of variance (One-wayANOVA), if significant difference remakes Duncan ' s multiple ratio, P＜0.05 expression otherness is remarkable.The result is as shown in Figure 5, from Fig. 5, finds out, the T-AOC activity of G1 group significantly is lower than G3, G5 organizes (P＜0.05), a little less than G2, G4 group.
This shows that selenium methionine and vitamin E can improve the T-AOC ability of prawn, the immune fatigue problem when the alleviation beta glucan adds separately through anti-oxidant approach.
6, the mensuration of Environment of Litopenaeus vannamei Low Content of MDA
MDA (MDA) is the primary product of peroxidatic reaction of lipid; The mensuration of MDA usually cooperatively interacts with the mensuration of superoxide dismutase (SOD); The height indirect reaction of SOD vigor body remove the ability of oxygen radical, and the height indirect reaction of MDA the body cell order of severity that attacked by free radical.
Measuring principle: the MDA in the lipid peroxide catabolite can with the thiobarbituricacid condensation, form red product, at the 532nm place maximum absorption band is arranged.Can obtain MDA content in each sample through colorimetric.
This test serum sample is stored in-80 ℃ of Environment of Litopenaeus vannamei Low blood serum samples in the refrigerator among the embodiment 1.
This is tested used MDA (MDA) and measures kit and build up bio-engineering research institute available from Nanjing (title: MDA (MDA) testing cassete (/ 96 kinds of 100 pipes) article No.: A003-1), by specification is measured.
Result of the test is with mean+SD (means ± SD) expression.Adopt SPSS13.0 software to carry out data statistics and analysis, earlier data are carried out one-way analysis of variance (One-wayANOVA), if significant difference remakes Duncan ' s multiple ratio, P＜0.05 expression otherness is remarkable.The result is as shown in Figure 6, and as can beappreciated from fig. 6, the Content of MDA of G1, G2, G4, G5 group significantly is lower than G0 group and G3 group.
This shows that the two unites interpolation or glucan and selenium methionine beta glucan of the present invention or beta glucan and selenium methionine, the vitamin E three unites the generation that interpolation can suppress MDA, thereby improves the oxidation resistance of body.
7, the mensuration of Environment of Litopenaeus vannamei Low activity of SOD in serum
Superoxide dismutase (SOD) plays crucial effects to the oxidation and the anti-oxidant balance of body, and it can remove ultra-oxygen anion free radical, and the protection cell escapes injury.
Measuring principle: produce ultra-oxygen anion free radical through xanthine and xanthine oxidase reaction system, latter's oxidation azanol forms nitrite, under the effect of developer, presents aubergine, surveys its absorbance with visible spectrophotometer.When containing SOD in the sample; Then ultra-oxygen anion free radical there is narrow spectrum inhibitory action; The nitrite of formation is reduced, and the absorbance of measuring pipe during colorimetric is lower than the absorbance of control tube, calculates through formula and can obtain the SOD vigor in the sample.
This test serum sample is stored in-80 ℃ of Environment of Litopenaeus vannamei Low blood serum samples in the refrigerator among the embodiment 1.
This is tested used superoxide dismutase (SOD) and measures kit and build up bio-engineering research institute available from Nanjing (title: superoxide dismutase (SOD) testing cassete (enzyme linked immunosorbent assay) is article No. (96T): A001-3), the determination step by specification carries out.
Result of the test is with mean+SD (means ± SD) expression.Adopt SPSS13.0 software to carry out data statistics and analysis, earlier data are carried out one-way analysis of variance (One-wayANOVA), if significant difference remakes Duncan ' s multiple ratio, P＜0.05 expression otherness is remarkable.The result is as shown in Figure 7, and as can beappreciated from fig. 7, the serum T-SOD activity of G1, G2, G3 group is significantly higher than G0 group (P＜0.05), and the serum T-SOD activity of G2 group is significantly higher than other each groups.
This shows that the two unites the ability that interpolation can effectively improve body removing free radical beta glucan and selenium methionine.
Claims (7)
1. an Environment of Litopenaeus vannamei Low strengthens feed addictive with complex immunity, it is characterized in that, comprises beta glucan and selenium methionine, and selenium methionine calculates with the amount of selenium, and beta glucan and selenium mass ratio are 150 ~ 300:0.2.
2. Environment of Litopenaeus vannamei Low according to claim 1 strengthens feed addictive with complex immunity, it is characterized in that, also comprises vitamin E, and the mass ratio of beta glucan, selenium and vitamin E is: 150 ~ 300:0.2:100.
3. an Environment of Litopenaeus vannamei Low strengthens feed addictive with complex immunity, it is characterized in that, comprises beta glucan and vitamin E, and both mass ratioes are 150 ~ 300:100.
4. any described Environment of Litopenaeus vannamei Low of claim 1-3 strengthens feed addictive in the application as the feed addictive of Environment of Litopenaeus vannamei Low with complex immunity.
5. an Environment of Litopenaeus vannamei Low strengthens feed with complex immunity; It is characterized in that, comprise that common Environment of Litopenaeus vannamei Low basal feed and Environment of Litopenaeus vannamei Low strengthen feed addictive with complex immunity, described Environment of Litopenaeus vannamei Low strengthens feed addictive with complex immunity and comprises beta glucan and selenium methionine; The addition of beta glucan is the common Environment of Litopenaeus vannamei Low basal feed of 150 ~ 300mg/kg; Selenium methionine calculates with the amount of selenium, and addition is the common Environment of Litopenaeus vannamei Low basal feed of 0.2mg/kg.
6. Environment of Litopenaeus vannamei Low according to claim 5 strengthens feed with complex immunity; It is characterized in that; Described Environment of Litopenaeus vannamei Low strengthens feed addictive with complex immunity and also comprises vitamin E, and the addition of vitamin E is the common Environment of Litopenaeus vannamei Low basal feed of 100mg/kg.
7. an Environment of Litopenaeus vannamei Low strengthens feed with complex immunity; It is characterized in that; Comprise that common Environment of Litopenaeus vannamei Low basal feed and Environment of Litopenaeus vannamei Low strengthen feed addictive with complex immunity; Described Environment of Litopenaeus vannamei Low strengthens feed addictive with complex immunity and comprises beta glucan and vitamin E, and the addition of beta glucan is the common Environment of Litopenaeus vannamei Low basal feed of 150 ~ 300mg/kg, and the addition of vitamin E is the common Environment of Litopenaeus vannamei Low basal feed of 100mg/kg.
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| CN104206691A (en) * | 2014-09-17 | 2014-12-17 | 中粮营养健康研究院有限公司 | Feed for penaeus vannamei and preparation method of feed for penaeus vannamei |
| CN106417985A (en) * | 2016-11-28 | 2017-02-22 | 宁夏农林科学院 | Compound polysaccharide immunity synergist for poultry and technology and application thereof |
| CN110800886A (en) * | 2019-11-06 | 2020-02-18 | 广东海洋大学 | Application of TWS119 in promoting growth of prawn and improving nonspecific immunity and disease resistance |
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