CN102732531B - Rice blast resistant gene RMg7, RMg8 or RMg9, and its application - Google Patents

Abstract

The invention which belongs to the technical field of genetic engineering concretely relates to a rice blast resistant gene RMg7, RMg8 or RMg9, and its application. The invention discloses nucleotide sequences of the new rice blast resistant genes RMg7, RMg8 and RMg9, and amino acid polypeptide sequences coded by the nucleotide sequences. The three genes belong to an NBS-LRR (nucleotide-binding site and leucine-rich-repeat) type disease resistant gene family. The three rice blast resistant genes of the invention are cloned from a rice line highly resisting rice blast pathogens, and are converted to lines which can suffer from the rice blast pathogens, and the disease resistances of the lines are assessed through using a rice blast pathogen invasion method, so a case that the three genes have resistances to the rice blast is finally determined.

Description

A kind of resistance gene of rice blast RMg7 or RMg8 or RMg9 and application thereof

Technical field

The invention belongs to gene engineering technology field, be specifically related to a kind of resistance gene of rice blast RMg7 or RMg8 or RMg9 and application thereof.

Background technology

Since first plant disease resistance genes Hm1 in 1992 by Johal and (Tanksley et al. 2007 since Briggs separates from corn; Dong Yuchen, 2001), up to the present, having surpassed 50 plant disease resistance genes is cloned from different plants and is separated, wherein the disease-resistant gene of main type is the disease-resistant gene of NBS-LRR structure type (Nucleotide-binding site and leucine-rich-repeat, i.e. nucleotide binding site and rich leucine repetitive proteins); Also have the small part disease-resistant gene to be mainly types such as eLRR-TM-pkinase, eLRR-TM, STK in addition.These disease-resistant genes are encoded respectively to the resistance protein of virus, bacterium, fungi, oomycetes even nematode and insect etc.

Because Plant diseases is annual produces for farming, woods to bring tremendous loss, controlling plant diseases is to ensure one of key issue that farming, woods Sustainable development institute must solutions rationally and effectively.Facts have proved that in a large number the existing plant disease-resistant germ plasm resource of development and utilization is (the Tanksley et al. 2007 of one of effective means the most of controlling plant diseases; Dong Yuchen, 2001).Traditional disease-resistant variety is cultivated, and continuous backcross breeding is passed through in normally that the kind of good quality and high output is strong with disease resistance material hybridization then, obtains the new variety of disease-resistant and high-quality at last.Although this method is very effective, extremely consuming time, after rearing new variety comes out, may show as susceptible (Tanksley et al. 2007) to pathogenic bacteria strain system or the microspecies that produce of newly evolving; Classical map based cloning method is utilized disease-resistant and susceptible mixing breed, then a large amount of separation offsprings is inoculated germ, identifies resistance, genetic mapping etc., clones on the basis of accurate heredity location at last.This method has obtained good effect, in the clone of plant disease resistance genes, played important effect (as separation and the utilization of bacterial leaf spot resistant ospc gene Xa21, Song et al. 1995), but because of its cycle grow, waste time and energy, separation and allelomorphism detect than reasons such as difficulties, is not suitable for cloning in a large number the needs of disease-resistant gene.Simultaneously, because of the mode of inheritance of disease-resistant gene uniqueness, also make the application of this method be subjected to very big restriction.Be example with the rice blast resistant gene, this genoid cluster (gene cluster) (Wang et al. 1999 that distributes usually in genome; Liu et al. 2002; Lin et al. 2007), the position between the homologous chromosomes of different varieties and structure also more is asymmetric distribution, and allelic relationship is indeterminate, big (the Yang et al. 2007 of copy number variation; Ding et al. 2007a; Sun et al. 2008; Li et al. 2010).These genetic phenomenons have increased the difficulty of map based cloning greatly, have had a strong impact on the cloning efficiency of disease-resistant gene.

In recent years, the breakthrough progress along with plant disease-resistant and the research of disease-resistant related gene molecular level makes us that the variation of essence arranged the understanding of structure, function, origin, variation and the preservation of plant disease-resistant and disease-resistant related gene.Being plant disease resistance genes, mainly is the gene that a class contains the LRR structural domain, wherein again based on NBS-LRR(nucleic acid in conjunction with-be rich in leucine) the type disease-resistant gene.Because these genes all have the similarity of height on structure and function, in conjunction with heredity and the evolution feature of its uniqueness, provide convenience for cloning fast with identifying these genes, also for utilizing disease-resistant germ plasm resource that new opportunity is provided efficiently.

Paddy rice ( Oryza sativa) be one of most important food crop in the world, also be one of topmost raise crop of China simultaneously, but all be subjected to the invasion of serious disease and pest its every year, brought tremendous loss to agriculture production.Wherein, rice blast is one of the widest, the severe diseases of hazard rice that distributes, badly influence the output of paddy rice, the annual rice yield loss that is caused by rice blast in the whole world accounts for 11-30% and (is roughly equal to 1.57 hundred million dollars, http://www.fungalgenomics.ncsu.edu), directly threatening the rice farming increases income and national grain security.No matter in the world still in China, solve a rice blast difficult problem and also be always the problem of a preferential research that ensures that paddy rice continues to produce.

One of greatest difficulty of rice blast control is germ variation itself and differentiation speed fast (Ling Zhongzhuan etc., 2004).New physiological races of rice blast fungus emerges in an endless stream, the very fast forfeiture resistance of existing disease-resistant gene (Plant disease resistance, R gene), and it is more and more difficult to seek new disease-resistant material, rice blast is also increasing to the threat of Rice Production.Corresponding variable pathogenic bacteria, paddy rice must have a lot of disease-resistant genes.From 70-80 the gene locus of having located, rice blast resistant gene One's name is legion.But, only 14 of the blast resistant genes of up to the present having cloned.Though the gene of having located can be used by the method for molecular marker assisted selection, this seed selection process is loaded down with trivial details, consuming time, after rearing new variety comes out, may show as the dissociant of new generation susceptible, clone and directly to transform disease-resistant gene may be the approach of the most direct effective cultivation disease-resistant variety.Under such background, use new thinking, research and develop out can blast resistant gene new technology, just seem particularly important and have inestimable value.

The present invention be exactly adopt a kind of efficiently, the cloning process of disease-resistant gene fast, employing is screened potential disease-resistant gene with bioinformatics method, and utilize a large amount of, the quick clone candidate disease-resistant gene of Protocols in Molecular Biology, finally in paddy rice, obtain a plurality of genes with rice blast resistance.

Summary of the invention

The objective of the invention is the rice blast resistance gene RMg7 in the high anti-kind of separating rice, RMg8 and RMg9 and comprise the dna fragmentation of the promotor of regulating and control these three genes.

Another object of the present invention provides above-mentioned rice blast resistance gene RMg7, the protein sequence that RMg8 and RMg9 are coded.

Another object of the present invention provides the carrier that contains above-mentioned resistant gene.

Another object of the present invention provides the transfer-gen plant that above-mentioned carrier transforms.

Another object of the present invention provides the application of above-mentioned protein in preparation resisting rice blast bacteria medicine.

The present invention relates to the clone and identify that a kind of RMg7 of comprising, the dna fragmentation of RMg8 and RMg9 gene, the albumen of these genes encodings can make paddy rice that the caused disease of Pyricularia oryzae is produced specific disease resistance response.Wherein, described fragment is respectively as sequence table SEQ ID NO:1, shown in SEQ ID NO:4 and the SEQ ID NO:7 or be equivalent to SEQ ID NO:l basically, DNA sequence shown in SEQ ID NO:4 and the SEQ ID NO:7, perhaps its function is equivalent to SEQ ID NO:l, the subfragment of sequence shown in SEQ ID NO:4 and the SEQ ID NO:7.These dna sequence dnas a kind of NBS-LRR proteinoid of all encoding, its aminoacid sequence is respectively as SEQ ID NO:2, shown in SEQ ID NO:5 and the SEQ ID NO:8.

The RMg7 that separates, clones, RMg8 and RMg9 resistant gene coding NBS-LRR albumen.Their albumen all comprises two main structural domain: NBS and LRR zone, the N end of RMg7 albumen has tangible CC structural domain, and the C-end is that 20 LRR repeat, and the N end of RMg8 albumen also is tangible CC structural domain, the C end is that 27 LRR repeat, and the C end of RMg9 albumen is that 21 LRR repeat.

According to RMg7 provided by the invention, RMg8 and RMg9 gene order information (SEQ ID NO:l, SEQ ID NO:4 and SEQ ID NO:7), those skilled in the art can obtain and RMg7 by the following method, the gene that RMg8 and RMg9 are equal to: (1) obtains by database retrieval; (2) with RMg7, RMg8 and RMg9 gene fragment are that genomic library or the cDNA library of probe Screening of Rice or other plant obtains; (3) according to RMg7, RMg8 and RMg9 gene order information design oligonucleotides primer obtain from genome, mRNA and the cDNA of paddy rice or other plant with the method for pcr amplification; (4) at RMg7, obtain with the gene engineering method transformation on the basis of RMg8 and RMg9 gene order; (5) method with chemosynthesis obtains this gene.

Rice blast resistance gene RMg7 provided by the invention, RMg8 and RMg9 have important use and are worth.With described RMg7, RMg8 and RMg9 gene order import paddy rice or other plant cell with any method for transformation, can obtain the transgenosis disease-resistant variety of expressing said gene, thereby are applied to produce.Of the present invention gene constructed in plant conversion carrier, can suitably modify described gene or its regulating and controlling sequence, also can replace the original promotor of described gene with other promotor, thereby widen anti-spectrum or strengthen resistance.

The present invention has following beneficial effect: clone's blast resistant gene is changed in the susceptible plant, help to obtain new disease-resistant plants.Clone's disease-resistant gene can shift between different species and utilize, thereby can overcome the difficulty of distant hybirdization in traditional breeding for disease resistance.In addition, can shorten breeding cycle with transgenic technology a plurality of disease-resistant genes that in plant, add up.The present invention can also further provide or use disease-resistant transgenic plant and the corresponding seed that above-mentioned dna fragmentation obtains, and with gene of the present invention or based on the recombinant chou plants transformed of this gene or the seed that is obtained by this class plant.Can change gene of the present invention over to other plant with the mode of sexual hybridization.

Description of drawings

Fig. 1 is schematic flow sheet of the present invention.Figure 1A: the determining of blast resisting candidate locus; Figure 1B-E: the separation of blast resistant gene and clone; Fig. 1 F-G: the genetic transformation of blast resistant gene; Fig. 1 H: the evaluation of transformant.

Fig. 2 is that the hygromycin gene of the transformant of rice blast resistance gene RMg7 among the embodiment one and the PCR of CaMV 35S promotor detect electrophorogram, Fig. 2 A is the pcr amplification product of CaMV 35S promotor, swimming lane 1-6 is the PCR test of resistant plants Seven amplified production, swimming lane 7 is the PCR product of carrier pCAMBIA1300-AscI plasmid, and swimming lane 8 is the non-transformant PCR product of acceptor; Fig. 2 B is the PCR product of hygromycin gene, and swimming lane 1-6 is the PCR test of resistant plants Seven amplified production, and swimming lane 7 is the PCR product of carrier pCAMBIA1300-AscI plasmid, and swimming lane 8 is the non-transformant PCR product of acceptor;

Fig. 3 is that the resistance of the transformed plant of rice blast resistance gene RMg7 among the embodiment one is identified figure, and Fig. 3 A is the new No. 2 resistance evaluation figure of adjoining tree, and Fig. 3 B is the new No. 2 resistance evaluation figure of transfer-gen plant;

Fig. 4 is that the hygromycin gene of the transformant of rice blast resistance gene RMg8 among the embodiment two and the PCR of CaMV 35S promotor detect electrophorogram, Fig. 4 A is the pcr amplification product of CaMV 35S promotor, swimming lane 1-3 and 5-7 are the PCR test of resistant plants Seven amplified production, swimming lane 4 is the non-transformant PCR product of acceptor, and swimming lane 8 is the PCR product of carrier pCAMBIA1300-AscI plasmid; Fig. 4 B is the PCR product of hygromycin gene, and swimming lane 1-3 and 5-7 are the PCR test of resistant plants Seven amplified production, and swimming lane 4 is the non-transformant PCR product of acceptor, and swimming lane 8 is the PCR product of carrier pCAMBIA1300-AscI plasmid;

Fig. 5 is that the resistance of the transformed plant of rice blast resistance gene RMg8 among the embodiment one is identified figure, and Fig. 5 A is the new No. 2 resistance evaluation figure of adjoining tree, and Fig. 5 B is the new No. 2 resistance evaluation figure of transfer-gen plant;

Fig. 6 is that the hygromycin gene of the transformant of rice blast resistance gene RMg9 among the embodiment three and the PCR of CaMV 35S promotor detect electrophorogram, Fig. 6 A is the pcr amplification product of CaMV 35S promotor, swimming lane 1-5 is the PCR test of resistant plants Seven amplified production, swimming lane 6 is the non-transformant PCR product of acceptor, and swimming lane 7 is the PCR product of carrier pCAMBIA1300-AscI plasmid; Fig. 6 B is the PCR product of hygromycin gene, and swimming lane 1-5 is the PCR test of resistant plants Seven amplified production, and swimming lane 6 is the non-transformant PCR product of acceptor, and swimming lane 7 is the PCR product of carrier pCAMBIA1300-AscI plasmid;

Fig. 7 is that the resistance of the transformed plant of rice blast resistance gene RMg9 among the embodiment one is identified figure, and Fig. 7 A is the resistance evaluation figure in the adjoining tree Taibei 309, and Fig. 7 B is the resistance evaluation figure in the transfer-gen plant Taibei 309;

Embodiment

Determining of rice anti-rice blast candidate gene: fine based on paddy rice genome sequencing kind 93-11 and Japan, the disease-resistant gene of all NBS-LRR types in the identified gene group at first, on the basis of systematic evolution tree, our picked at random 60 candidates' blast resistant gene site, pass through design of primers, in the rice varieties of blast resisting, carry out long segment PCR, vector construction, transgenosis, processes such as resistance evaluation, derive from the distribute Pyricularia oryzae system of all paddy rice main producing regions of China's Mainland by 10 at last and identify, identify 3 blast resistant genes with specificity resistance.

Method therefor is ordinary method if no special instructions among the following embodiment.

Embodiment one:Rice blast resistance gene RMg7(Os11g37780-GM) clone and evaluation

1, determining of blast resisting candidate locus RMg7: (1) many forms with gene family and gene cluster exist, and 9 and 11 close copies are respectively arranged in the warm and fine 93-11 of Japan; (2) has higher Ka/Ks value in its LRR zone, particularly xxLxLxx zone;

2, the separation of rice blast resistance gene RMg7 and clone: utilizing public database order-checking kind Japan fine (Nipponbare) and 93-11 is reference sequences, design primer (the primer two ends all have restriction enzyme site AscI).The forward primer sequence is SEQ ID NO:10, and the reverse primer sequence is SEQ ID NO:11.With disease resisting rice kind Tetep, Gumei2 and Q2436 are template, utilize long segment round pcr (Long-PCR) amplification candidate gene fragment.The PCR program is as follows: 95 ℃ of pre-sex change 5 minutes, and 95 ℃ of sex change 30 seconds, 60 ℃ of renaturation 45 seconds, 68 ℃ were extended 5.5 minutes, totally 35 circulations, 72 ℃ of insulations subsequently 10 minutes, last 10 ℃ of constant temperature.Subsequently to the recovery of tapping rubber of PCR product.

3, the preparation of difunctional basic carrier: rare restriction enzyme site AscI is imported between the multiple clone site BamHI and SalI of bifunctional vector pCAMBIA1300, replace restriction enzyme site XbaI, formation base carrier pCAMBIA1300-AscI.

4, being connected of candidate resistance gene and carrier is carrier: use restriction enzyme A scI, enzyme is cut PCR product and carrier is carrier plasmid simultaneously, and purifying.Under the effect of T4 ligase enzyme, the candidate gene fragment is connected into carrier is carrier pCAMBIA1300-AscI.

5, the genetic transformation of candidate's disease-resistant gene: the bifunctional vector that will carry candidate gene imports agrobacterium tumefaciens EHA105, adopts agrobacterium-mediated transformation, candidate gene is transformed into the general sense kind of paddy rice new No. 2, among TP309 and the Co39.Obtain independently transformant of 20 strains at last altogether.

6, PCR Molecular Detection: be template with transformant DNA, with the Auele Specific Primer of hygromycin gene and upstream CaMV35S promotor thereof it carried out the PCR reaction.The forward primer sequence of hygromycin gene is SEQ ID NO:16, and the reverse primer sequence is SEQ ID NO:17, and the forward primer sequence of CaMV35S promotor is SEQ ID NO:18, and the reverse primer sequence is SEQ ID NO:19.

7, the resistance of transformant is identified: selects 10 source place differences, the good independent physiological races of rice blast fungus of perspective is as the pathogen species of detection.Utilize the inoculation of Pyricularia oryzae microspecies to infect T2 for transfer-gen plant and check variety, the transformant that the screening disease resistance changes.Resistance qualification result show candidate gene RMg7 to 6 pathogenic strains resistance of showed different, shows that candidate gene RMg7 has the specificity resistance and successfully cloned under new No. 2 genetic background of general sense kind.

8, the gene structure of rice blast resistance gene RMg7 and protein structure analysis: adopt walking method that the dna sequence dna of RMg7 is checked order.RMg7 gene DNA length is 3967bp, contains 3 opening code-reading frames, 2 introns and 3 exons.RMg7 encoded protein sequence is shown in SEQ ID NO:2.1 protein polypeptide of being formed by 961 amino-acid residues of RMg7 genes encoding.Utilize this protein polypeptide of COIL analysis revealed that CC(coiled-coil is arranged) structural domain.RMg7 albumen belongs to NBS-LRR albumen, and the C-end of its albumen is that 20 LRR repeat.

Embodiment two:Rice blast resistance gene RMg8(Os12g03080-Tetep) clone and evaluation

1, determining of blast resisting candidate locus RMg8: (1) exists with the single-gene form, and 1 copy is respectively arranged in the warm and fine 93-11 of Japan; (2) has higher Ka/Ks value in its LRR zone, particularly xxLxLxx zone;

2, the separation of rice blast resistance gene RMg8 and clone: utilizing public database order-checking kind Japan fine (Nipponbare) and 93-11 is reference sequences, design primer (the primer two ends all have restriction enzyme site AscI).The forward primer sequence is SEQ ID NO:12, and the reverse primer sequence is SEQ ID NO:13.With disease resisting rice kind Tetep, Gumei2 and Q2436 are template, utilize long segment round pcr (Long-PCR) amplification candidate gene fragment.The PCR program is as follows: 95 ℃ of pre-sex change 5 minutes, and 95 ℃ of sex change 30 seconds, 60 ℃ of renaturation 45 seconds, 68 ℃ were extended 10.5 minutes, totally 35 circulations, 72 ℃ of insulations subsequently 10 minutes, last 10 ℃ of constant temperature.Subsequently to the recovery of tapping rubber of PCR product.

3, the preparation of difunctional basic carrier: rare restriction enzyme site AscI is imported between the multiple clone site BamHI and SalI of bifunctional vector pCAMBIA1300, replace restriction enzyme site XbaI, formation base carrier pCAMBIA1300-AscI.

4, being connected of candidate resistance gene and carrier is carrier: use restriction enzyme A scI, enzyme is cut PCR product and carrier is carrier plasmid simultaneously, and purifying.Under the effect of T4 ligase enzyme, the candidate gene fragment is connected into carrier is carrier pCAMBIA1300-AscI.

5, the genetic transformation of candidate's disease-resistant gene: the bifunctional vector that will carry candidate gene imports agrobacterium tumefaciens EHA105, adopts agrobacterium-mediated transformation, candidate gene is transformed into the general sense kind of paddy rice new No. 2, among TP309 and the Co39.Obtain independently transformant of 20 strains at last altogether.

6, PCR Molecular Detection: be template with transformant DNA, with the Auele Specific Primer of hygromycin gene and upstream CaMV35S promotor thereof it carried out the PCR reaction.The forward primer sequence of hygromycin gene is SEQ ID NO:16, and the reverse primer sequence is SEQ ID NO:17, and the forward primer sequence of CaMV35S promotor is SEQ ID NO:18, and the reverse primer sequence is SEQ ID NO:19.

7, the resistance of transformant is identified: selects 10 source place differences, the good independent physiological races of rice blast fungus of perspective is as the pathogen species of detection.Utilize the inoculation of Pyricularia oryzae microspecies to infect T2 for transfer-gen plant and check variety, the transformant that the screening disease resistance changes.Resistance qualification result show candidate gene RMg8 to 4 pathogenic strains resistance of showed different, shows that candidate gene RMg8 has the specificity resistance and successfully cloned under new No. 2 genetic background of general sense kind.

8, the gene structure of rice blast resistance gene RMg8 and protein structure analysis: adopt walking method that the dna sequence dna of RMg8 is checked order.RMg8 gene DNA length is 5344bp, contains 6 opening code-reading frames, 5 introns and 6 exons.RMg8 encoded protein sequence is shown in SEQ ID NO:5.1 protein polypeptide of being formed by 1128 amino-acid residues of RMg8 genes encoding.Utilize this protein polypeptide of COIL analysis revealed that CC(coiled-coil is arranged) structural domain.RMg8 albumen belongs to NBS-LRR albumen, and the C-end of its albumen is that 27 LRR repeat.

Embodiment three:Rice blast resistance gene RMg9(Os06g06390-GM) clone and evaluation

1, determining of blast resisting candidate locus RMg9: (1) many forms with gene family and gene cluster exist, and 2 close copies are respectively arranged in the warm and fine 93-11 of Japan; (2) has higher Ka/Ks value in its LRR zone, particularly xxLxLxx zone;

2, the separation of rice blast resistance gene RMg9 and clone: utilizing public database order-checking kind Japan fine (Nipponbare) and 93-11 is reference sequences, design primer (the primer two ends all have restriction enzyme site AscI).The forward primer sequence is SEQ ID NO:14, and the reverse primer sequence is SEQ ID NO:15.With disease resisting rice kind Tetep, Gumei2 and Q2436 are template, utilize long segment round pcr (Long-PCR) amplification candidate gene fragment.The PCR program is as follows: 95 ℃ of pre-sex change 5 minutes, and 95 ℃ of sex change 30 seconds, 60 ℃ of renaturation 45 seconds, 68 ℃ were extended 7.5 minutes, totally 35 circulations, 72 ℃ of insulations subsequently 10 minutes, last 10 ℃ of constant temperature.Subsequently to the PCR product purifying of tapping rubber.

3, the preparation of difunctional basic carrier: rare restriction enzyme site AscI is imported between the multiple clone site BamHI and SalI of bifunctional vector pCAMBIA1300, replace restriction enzyme site XbaI, formation base carrier pCAMBIA1300-AscI.

4, being connected of candidate resistance gene and carrier is carrier: use restriction enzyme A scI, enzyme is cut PCR product and carrier is carrier plasmid simultaneously, and purifying.Under the effect of T4 ligase enzyme, the candidate gene fragment is connected into carrier is carrier pCAMBIA1300-AscI.

5, the genetic transformation of candidate resistance gene: the bifunctional vector that will carry candidate gene imports agrobacterium tumefaciens EHA105, adopts agrobacterium-mediated transformation, candidate gene is transformed into the general sense kind of paddy rice new No. 2, among TP309 and the Co39.Obtain independently transformant of 20 strains at last altogether.

6, PCR Molecular Detection: be template with transformant DNA, with the Auele Specific Primer of hygromycin gene and upstream CaMV35S promotor thereof it carried out the PCR reaction.The forward primer sequence of hygromycin gene is SEQ ID NO:16, and the reverse primer sequence is SEQ ID NO:17, and the forward primer sequence of CaMV35S promotor is SEQ ID NO:18, and the reverse primer sequence is SEQ ID NO:19.

7, the resistance of transformant is identified: selects 10 source place differences, the good independent physiological races of rice blast fungus of perspective is as the pathogen species of detection.Utilize the inoculation of Pyricularia oryzae microspecies to infect T2 for transfer-gen plant and check variety, the transformant that the screening disease resistance changes.Resistance qualification result show candidate gene RMg9 to 4 pathogenic strains resistance of showed different, shows that candidate gene RMg9 has specificity and successfully cloned under the genetic background in the general sense kind Taibei 309.

8, the gene structure of rice blast resistance gene RMg9 and protein structure analysis: adopt walking method that the dna sequence dna of RMg9 is checked order.RMg9 gene DNA length is 3866bp, contains 2 opening code-reading frames, 1 one introns and 2 exons.RMg9 encoded protein sequence is shown in SEQ ID NO:8.1 protein polypeptide of being formed by 989 amino-acid residues of RMg9 genes encoding.RMg9 albumen belongs to NBS-LRR albumen, and the C-end of its albumen is that 21 LRR repeat.

＜110〉Nanjing University

＜120〉a kind of resistance gene of rice blast RMg7 or RMg8 or RMg9 and application thereof

<160> 19

<210> 1

<211> 4528

<212> DNA

＜213〉Oryza paddy rice (Orysa sativa L.)

<400> 1

atggagttca ccacagggac aatgggcacc ctcctcccaa agttgggcaa gcttcttaag 60

gaagagtaca acctaaggaa aagtgtgaag gaaggaatca tattcctcaa ggccgagctc 120

gagagcatac aagctgtcct tgagaaggtc tcaatggtgc cactagatca acttgacaca 180

caaatcaagt tctgggctag ggatgtaagg gacatatcct acgatatcga ggacaccgtt 240

gataccttca tgctgcatgt caatggactc gagccaacaa acaaacacaa cttcacatgg 300

ttaatcaata agtgccacaa gtctttatcc aaagtaaaga tccaccacaa aatagccaac 360

gacatcaaat atctcaagag gcaaatcaag gaggtgatgg atagacgtga taggtacaag 420

attgacgata ttgttgctaa acctcgaaca gtgattgacc ctcgcctctt gacattatat 480

gagaaggcca ccaatctcat tggcgttgat aagccagctg ataatctaat caagatgcta 540

tctatcgggg atgagacatt gaggaatctc aaaatggttt ctgttgttgg atttggagga 600

ttgggcaaga caactcttgc caaagtagta tttgacatgc ttaaagggca attcgattgt 660

gccggttttg ttcctgtcgg tcagagacct gacatcaaga gagtttttaa gggcatccta 720

attgaagtta acaagcacaa gtacatggta tttgatgcat tggcattaaa tgaaaggcat 780

ttaattgatg aactccgaga atatcttgac aataggaggt atgtatgcaa tacccaccaa 840

atcaattgtt tgtcttgatc aacctaaggt aatggagttc tattagctac tagaattata 900

atttacttct ttctggttgc ggtagttgta catttagtaa attaggtgaa accccgcact 960

ttgctgtggg agtttattat gataacaata agtaattata acgtgtaaga caacttaaac 1020

aatataagct tacataaaca taagttcata taagttgatg tgatttaaat ttaaatagga 1080

tgtggtctga tgattcaaat gtaaaagtaa tgttatgtgc ctttacgaga gaacagaaga 1140

agaaagataa tttagaccat aaatctaagg gctaaaaaaa ttgagatgat atggcttagt 1200

gagagaagaa aagaagagag atattttgaa ccatagatga atcatctaag gataaaaaat 1260

aattgatatg atatgactta atgagagaaa agaaaaataa catgaactaa gtgaggatga 1320

attatatagg tatataggat attaaaaaaa tgatggaggt atattgaaat attatgtgaa 1380

ttatgtggga tgatttaaca tgcttacatt ttaaatctct aaaagttaat aaattattaa 1440

attattgata atatagtata tacttgcgtg atgcttaaat gtaataatta ttagtggatg 1500

atgatgtggc atctttacat gttaagcttt agaaattaga gggctataat tttatagtaa 1560

gagatgattt gtattagttg tattagtggt cgattcaatt tggtactgaa aatgggacac 1620

aagttaattt attcccctca tcaatcatcc tgttgatggg tgcttatgtc actatgatct 1680

ttgatgctta tacatttcta tctacatgga tatactaatt ctagcccctt ccattcccat 1740

gctgataggt atctgattat cattgatgac atatgggagt catcaacatg gaaaattatt 1800

aaatgtgctt tcttagattg taacagtgga agtagagtaa ttgcaacaac tcgtatttct 1860

caagttgcca aagaaattgc taaagaattt ggctatgttt acatcatgaa gccactttcc 1920

atcaataact caaaaaagtt attctgtagc agaatctttg gtgctgatta caatggtcca 1980

agtaataatc aaccggctga ggtaactaaa aagatcttaa agaaatgtgg tggcgtacca 2040

ttgtctataa ttacaatagc tagtttattg gttcataaac cattggagga ttggtccgac 2100

gtgtatgaat ctattggttt tgggtctaca gatcaaaatg aagtagttca taacacaaga 2160

aagatattgt cttttagtta ctatgatttg ccttcatacc taaagacttg tatgctccac 2220

ctgagcatat atccagaaga tcatttgatc gagaaagact gtttgatatg gaagtgggta 2280

gcagaaggat ttatccatga ggaacaaggg aaggggttat tcgagaccgg agaaaggtat 2340

ttcattgagc tcataaataa aagcatgatc caaccaacag aacaaaccct ttttggctgc 2400

aaagtggatg gttgtcgtgt ccatgacatg gtgctgggcc tcatccgcat cttggcaaca 2460

gaagaaaact ttttagaagt actgcataga gggcatgagc agcagagccc atctttgcac 2520

agcaaaacaa tccgcaggct agcacttcat aagagtcgga accaagataa ctttgccata 2580

ggcatggagc agctgagatt gttcaatgcc atcgagtgcc ctattaacat ggcttccccc 2640

cttgtgaact cccacgttct acgtgtgcta gccttagaaa attgtgttgt catgggaggt 2700

tgcctcaagc atctcagaaa actgcttcag ttgaggtacc taggattgag atacacacgt 2760

attgatgagc tcccaagtga aataggagat ctagtgcact tgcaaacact agacataatg 2820

aacaccgggt tagaggcact cccagcgaca attggcaagc ttaccaagct gatgcgtctg 2880

cttgctgaca atggcacaag agttcatgcc ggggtagcga atctgacatc cctccaagag 2940

ctgttgctag gaaaaatttc cgacgacacc tgcccaaact ttgccgtgga gctgtgcaca 3000

ttgacagact tgagggtgct caatatttgg atcaagatgg aagatgaggg cacattgaac 3060

acactagtgg agtctctgca gagtctacgt agaattcaga acctaaatat tcgtctcgac 3120

ataaaggaca ctggcatgga gacaaagtac tccatgatgt gtatttggga agactgggag 3180

cccccacggc agcttcatga gttctgtctg agtggttgga ctggtttctt gcctcgacta 3240

ccggcctgga tgaactccaa gcgcatcccg aacctctcca aactggacat gaaagtgctt 3300

gccatggaac catgggacct agacaccctt gggagaatgc cgatgctcca ctacctccgc 3360

atgcgtattt gcacgaagat atcttcatgg acagttggtt gtggattgtt cccaaacctg 3420

cgatactgca tgatgaacat agagctcaag tttctgcagg gagcgatgcc aatgctcatg 3480

gagattgaat tttatgtgtc ggcgtcgagg cctggtgttc ccaacgatgt tggattgggg 3540

aacctcccgc tgctcaactg tgcccatatt tcccttgagt gcaagggtgc gacagggagg 3600

cagatggagg aagaagagat tgcactgagg cgctatcacc cgaatcgtcc tgccatttat 3660

gtgttccgct tagatgaggt tcgattggtt ttgttagtca tcatgatcat ttgattgatt 3720

agttagttga agttcacata agcactccca caattaatca tattttttct caattcgtca 3780

attacattcc tcaactcaca tctcagggcc ctctcaattt caatttcagc atctgatgaa 3840

aagggacaaa gatgatggtg atgagaagat ttcagctgcg ggccaagttg taaagcactt 3900

tcctggtgtg cagacaatca ggaggaggag gttgcgcaaa caggacgaag gtgttcacca 3960

atattgaacg aacccgtttt ttgtcagcat gcggtttact catgcaggta cacgtcgaaa 4020

aagcggcacc ccaaaatttt ggcattgtgt ccggctatat atctcttttg cacaaatttt 4080

ggcataaaat ggaacctacg gtaccttacg agaccttggt atggcaccac acaaaccggc 4140

tccaaattcg ttcttgttat ccgtttttgg ttgcttccct gttctccatt attctgttga 4200

gagctcggct tgtttttatg caagtacttc ctccgtttcg caatgtaagt cattctagca 4260

ttttccacat tcatatcgat gttaatgaat ctagatacat cgatatgaat gtggaaaatg 4320

ctagaatgac ttatattgtg aaacggatgg agtagcaatg aagaaaacat gaaaatctgc 4380

ggcagttttg cagcacaaaa cgccctgcgt atatgttctt ttttgtgcgt gcgtgtgaat 4440

tgtgtgaagg agctgggctg acaatcctgc atgaaaaagg attcaatttc gatagtggca 4500

cttgtggatt tagccttatc gggtcttc 4528

<210> 2

<211> 961

<212> PRT

＜213〉Oryza paddy rice (Orysa sativa L.)

<400> 2

Met Glu Phe Thr Thr Gly Thr Met Gly Thr Leu Leu Pro Lys Leu

1 5 10 15

Gly Lys Leu Leu Lys Glu Glu Tyr Asn Leu Arg Lys Ser Val Lys

20 25 30

Glu Gly Ile Ile Phe Leu Lys Ala Glu Leu Glu Ser Ile Gln Ala

35 40 45

Val Leu Glu Lys Val Ser Met Val Pro Leu Asp Gln Leu Asp Thr

50 55 60

Gln Ile Lys Phe Trp Ala Arg Asp Val Arg Asp Ile Ser Tyr Asp

65 70 75

Ile Glu Asp Thr Val Asp Thr Phe Met Leu His Val Asn Gly Leu

80 85 90

Glu Pro Thr Asn Lys His Asn Phe Thr Trp Leu Ile Asn Lys Cys

95 100 105

His Lys Ser Leu Ser Lys Val Lys Ile His His Lys Ile Ala Asn

110 115 120

Asp Ile Lys Tyr Leu Lys Arg Gln Ile Lys Glu Val Met Asp Arg

125 130 135

Arg Asp Arg Tyr Lys Ile Asp Asp Ile Val Ala Lys Pro Arg Thr

140 145 150

Val Ile Asp Pro Arg Leu Leu Thr Leu Tyr Glu Lys Ala Thr Asn

155 160 165

Leu Ile Gly Val Asp Lys Pro Ala Asp Asn Leu Ile Lys Met Leu

170 175 180

Ser Ile Gly Asp Glu Thr Leu Arg Asn Leu Lys Met Val Ser Val

185 190 195

Val Gly Phe Gly Gly Leu Gly Lys Thr Thr Leu Ala Lys Val Val

200 205 210

Phe Asp Met Leu Lys Gly Gln Phe Asp Cys Ala Gly Phe Val Pro

215 220 225

Val Gly Gln Arg Pro Asp Ile Lys Arg Val Phe Lys Gly Ile Leu

230 235 240

Ile Glu Val Asn Lys His Lys Tyr Met Val Phe Asp Ala Leu Ala

245 250 255

Leu Asn Glu Arg His Leu Ile Asp Glu Leu Arg Glu Tyr Leu Asp

260 265 270

Asn Arg Arg Tyr Leu Ile Ile Ile Asp Asp Ile Trp Glu Ser Ser

275 280 285

Thr Trp Lys Ile Ile Lys Cys Ala Phe Leu Asp Cys Asn Ser Gly

290 295 300

Ser Arg Val Ile Ala Thr Thr Arg Ile Ser Gln Val Ala Lys Glu

305 310 315

Ile Ala Lys Glu Phe Gly Tyr Val Tyr Ile Met Lys Pro Leu Ser

320 325 330

Ile Asn Asn Ser Lys Lys Leu Phe Cys Ser Arg Ile Phe Gly Ala

335 340 345

Asp Tyr Asn Gly Pro Ser Asn Asn Gln Pro Ala Glu Val Thr Lys

350 355 360

Lys Ile Leu Lys Lys Cys Gly Gly Val Pro Leu Ser Ile Ile Thr

365 370 375

Ile Ala Ser Leu Leu Val His Lys Pro Leu Glu Asp Trp Ser Asp

380 385 390

Val Tyr Glu Ser Ile Gly Phe Gly Ser Thr Asp Gln Asn Glu Val

395 400 405

Val His Asn Thr Arg Lys Ile Leu Ser Phe Ser Tyr Tyr Asp Leu

410 415 420

Pro Ser Tyr Leu Lys Thr Cys Met Leu His Leu Ser Ile Tyr Pro

425 430 435

Glu Asp His Leu Ile Glu Lys Asp Cys Leu Ile Trp Lys Trp Val

440 445 450

Ala Glu Gly Phe Ile His Glu Glu Gln Gly Lys Gly Leu Phe Glu

455 460 465

Thr Gly Glu Arg Tyr Phe Ile Glu Leu Ile Asn Lys Ser Met Ile

470 475 480

Gln Pro Thr Glu Gln Thr Leu Phe Gly Cys Lys Val Asp Gly Cys

485 490 495

Arg Val His Asp Met Val Leu Gly Leu Ile Arg Ile Leu Ala Thr

500 505 510

Glu Glu Asn Phe Leu Glu Val Leu His Arg Gly His Glu Gln Gln

515 520 525

Ser Pro Ser Leu His Ser Lys Thr Ile Arg Arg Leu Ala Leu His

530 535 540

Lys Ser Arg Asn Gln Asp Asn Phe Ala Ile Gly Met Glu Gln Leu

545 550 555

Arg Leu Phe Asn Ala Ile Glu Cys Pro Ile Asn Met Ala Ser Pro

560 565 570

Leu Val Asn Ser His Val Leu Arg Val Leu Ala Leu Glu Asn Cys

575 580 585

Val Val Met Gly Gly Cys Leu Lys His Leu Arg Lys Leu Leu Gln

590 595 600

Leu Arg Tyr Leu Gly Leu Arg Tyr Thr Arg Ile Asp Glu Leu Pro

605 610 615

Ser Glu Ile Gly Asp Leu Val His Leu Gln Thr Leu Asp Ile Met

620 625 630

Asn Thr Gly Leu Glu Ala Leu Pro Ala Thr Ile Gly Lys Leu Thr

635 640 645

Lys Leu Met Arg Leu Leu Ala Asp Asn Gly Thr Arg Val His Ala

650 655 660

Gly Val Ala Asn Leu Thr Ser Leu Gln Glu Leu Leu Leu Gly Lys

665 670 675

Ile Ser Asp Asp Thr Cys Pro Asn Phe Ala Val Glu Leu Cys Thr

680 685 690

Leu Thr Asp Leu Arg Val Leu Asn Ile Trp Ile Lys Met Glu Asp

695 700 705

Glu Gly Thr Leu Asn Thr Leu Val Glu Ser Leu Gln Ser Leu Arg

710 715 720

Arg Ile Gln Asn Leu Asn Ile Arg Leu Asp Ile Lys Asp Thr Gly

725 730 735

Met Glu Thr Lys Tyr Ser Met Met Cys Ile Trp Glu Asp Trp Glu

740 745 750

Pro Pro Arg Gln Leu His Glu Phe Cys Leu Ser Gly Trp Thr Gly

755 760 765

Phe Leu Pro Arg Leu Pro Ala Trp Met Asn Ser Lys Arg Ile Pro

770 775 780

Asn Leu Ser Lys Leu Asp Met Lys Val Leu Ala Met Glu Pro Trp

785 790 795

Asp Leu Asp Thr Leu Gly Arg Met Pro Met Leu His Tyr Leu Arg

800 805 810

Met Arg Ile Cys Thr Lys Ile Ser Ser Trp Thr Val Gly Cys Gly

815 820 825

Leu Phe Pro Asn Leu Arg Tyr Cys Met Met Asn Ile Glu Leu Lys

830 835 840

Phe Leu Gln Gly Ala Met Pro Met Leu Met Glu Ile Glu Phe Tyr

845 850 855

Val Ser Ala Ser Arg Pro Gly Val Pro Asn Asp Val Gly Leu Gly

860 865 870

Asn Leu Pro Leu Leu Asn Cys Ala His Ile Ser Leu Glu Cys Lys

875 880 885

Gly Ala Thr Gly Arg Gln Met Glu Glu Glu Glu Ile Ala Leu Arg

890 895 900

Arg Tyr His Pro Asn Arg Pro Ala Ile Tyr Val Phe Arg Leu Asp

905 910 915

Glu His Leu Met Lys Arg Asp Lys Asp Asp Gly Asp Glu Lys Ile

920 925 930

Ser Ala Ala Gly Gln Val Val Lys His Phe Pro Gly Val Gln Thr

935 940 945

Ile Arg Arg Arg Arg Leu Arg Lys Gln Asp Glu Gly Val His Gln

950 955 960

Tyr

<210> 3

<211> 375

<212> DNA

＜213〉Oryza paddy rice (Orysa sativa L.)

<400> 3

atacctgact gcgtgataca tgtactttcc cttctctcta acagtttcag aagttttaat 60

atttattcaa tgaaatccgt gtgccctcat ttctttcaaa tttctccctt tggtgcaagc 120

atgctatggc caactcttat gccaaaattt tgcattcttc attctcatta aagagcgtgt 180

acactgccaa cggatgtctt catatgcttt tcattttacc agggcttttc aaaatgcttt 240

gtgcgaagtg tggcaaatcc tgcaaataga tattatgaaa gcaactgacc tgtagtgaac 300

caacaccata tcatcatagc attatcatca caagaaacac acacccgagc gagggaagga 360

gtgaacaatc ggaaa 375

<210> 4

<211> 8223

<212> DNA

＜213〉Oryza paddy rice (Orysa sativa L.)

<400> 4

atggcccaac gatgcagccg cgcggtcgtc gtcttccatc tccacgacga ggtttggtat 60

cgcatccctg atcccccacg cctacctttc tctctctctc tccctggccc gcccagacta 120

gatctattag gggggagctc aacttgttcg atacttcgat ccgcaacttg caactacagc 180

cccaaactag aaatcgctgt cctcatacag cccccatctg actaccaaca gctaagcaaa 240

actagattat tactatgatt tttgtgagga tcgtccttct ttgactggct tgccatagag 300

tggtttcgaa agtgacattt ccacaccagc tttctgtatt taaactgcaa ttggtggata 360

ttcgatttat attatatgtt gtgggcatgt gtttccaact cacccagcac tacaccaacc 420

caaattggtg gatactaatc acattttaag gtgacaaggg agctctaatg aatggagctg 480

ccagaaccac caccactctg caactaaatc ccaagaaaca ggtgttattt gtgttgcttg 540

ggacagtgag gggatactgc aggatcaagt cagacgacct gagatcaggt gtttaattac 600

ctcagctttt gtatccttca aaactaaatt tctcgcgttt ttatgttaag ataatgtgtt 660

ctccaaaatc tactccacca ctaatctctt ttgcaaatgg tttgtctttt gttcagcttt 720

tctcagggga ttgttctacc tcttctcagg gagctagtac tctactacag tactactaag 780

tacggatatt tacaattcaa tagaaacaca acttactgat ggttcttgtg tgctagaagc 840

tttcatgggc atctatcttc cgttatttac tagtttgctt tcttgtatat acatcagaac 900

ctgtcctcaa tgtatcacat acatgtacct cttggttcat catgatgtta tgaacaattg 960

aacaaaacaa gtgcatcttc aaagagatcg aagaagcaca acttcaacga agagaaggca 1020

aaggcttaac acctattttt ccatatcagg taccattaac cgggcgtcat ctgttacgct 1080

tttatatgtc tatcttgctc tgcacgtgta aaacatggtt gaacttagaa agaaaataac 1140

gataaggcat acaaacatat actagcgtta gttttcattg cataaaaaat aattacgaat 1200

attgcacctg aatcatttga aattcagtat gtagcatctc attctgaaat ttaatatgta 1260

ggatttgggc caaagagaag cttccaagtt tcaaacatat gggcatggaa gctgctgtag 1320

tatctggatt atttaagatt gtgggagaca agttagcacc acttgtaata agagagtata 1380

gttctcttat gggtgttacg aaagatctcc aggagcttca agatttagtt gaggagatca 1440

aaagcgggct acaggtagca gctgataatg caattggtaa taaaccacca tctaattggc 1500

taaaaaaatt gaaagatttt gcttacgatc ttgaagattt ggtacatgaa tttcatcttc 1560

agtctgagaa acatgacaca gataatgatc gtgacaagta tgctgtgctc aaatacatgc 1620

gcacaaaacc aaaactagcc atgtttcaac tcaaaatggc caacaagata aagacaatca 1680

aaaacagatt tgctgcaatt gtcgagcaaa gaggcgatgt caatactata ttgaatgcta 1740

taccaatcga tcataaataa aaacaagatt atcagtgagc catccttatt gggcaatgtt 1800

gatgactcaa aaatacatgt gagagatgag gagaagcaca agatcatacg taagttaata 1860

gatgaccaac agaaaatctc aatagtttca attgttggac ttggtggaac cggtaaaact 1920

actatggcca cacacatctg tcatgacaac aaaataaaag agcattttga aggctcaata 1980

ttttgggttc acgtgtcaca agaatttgat aataacaaac tcgtcggcaa gctgtatgaa 2040

gccattttaa agaaaacatc atatcttcgt accgaccaac agatggttga agcaatttca 2100

aacgagttga atgggaataa gtttctactt gtcttggatg atgcttggca caagaatcaa 2160

tatgactggg aacgattcat gctatatctt aagagtggat cacctggaag ccggattttg 2220

ctcacaactc gtgatctggg agttgcggag gcagtggaat ctacatgtac atatacgttg 2280

gctttcttgt cagatgagga cagctggaat ttatttcaac aaagtttaag gttggctgca 2340

aaaggtctgc cctctgaatt tgtagaaatt ggaagagaga ttattaaaaa atgtggtggg 2400

gtgccactgg cgattaaaat tcttgcaggt gtcctccgta acaagaaaac agtagatgca 2460

tggtgtgcct tacgtgatag taacatgtgg aatgttgatg atatagaaga tagagtgttt 2520

gcgtcattaa ggctgagcta tttccatctg cctgatcatc tgaagcaatg ttttgtatat 2580

tgctctatat ttcctaaagg ctacaagatc tacaaacacc agttgatcgg agagtggata 2640

gcaaatggct tcatcaatcc aatgaatgag attgaacaag tagaggatgt tgcaaatgac 2700

tgctttgatt ctcttctgaa agtgcacttt ctccaagatc taggggtaga tgagtatgat 2760

gaaatggaaa tatgcaagat gcatgacctg gttcatgacc ttattcggca aattttacag 2820

ggtgaaatgg tgtctcattc tcagaatgca actattggta attcccaaaa atgcagatat 2880

ttatctttgg cttcgtgcaa tgagaatatt gaagtgaagt tatttagcaa ggtccatgct 2940

atctatattt ctggtgacaa cttcgcactc aacaagccaa tcaagaaaag gtgccatgtt 3000

cgttctatta ttttggagta tgggcgctac taatttgttg cttccattga taccaaagtt 3060

cgaatatctt agctattttc gtatctcaca cgctagctgt cgagcctttc cagaagaaat 3120

ctcacattgc tggaacttgc aggcccttca tgttacatat tgcagggcac tttccacatt 3180

acctgagtct attggtaagc ttaagaagct gagaacccta gagttgtcat gtcttttgga 3240

tcttgaaagt ttgcctcagt ccattggtga ctgtcataat cttcaaagct ttctcctgcg 3300

tggcagtggt atcagggaga taccaaactc catatgtaaa attaaaaaac taagagtgct 3360

taacattatg cattgtcgct ctttgaggca acaatggtca gaattctttg ggacgctttg 3420

caacttacag tccatcaact tagcacagat tgagggtatc cacaacttgt tcagttcgtt 3480

tgcttgtcat aaactgcgca ctttgacact tagtggaacc gaaattacca gactaccgca 3540

atgcctcaca ttggtgagca ccctggaata catagacctt caaaactgct gggggctgct 3600

agagttatcg gagggcatag ggaacctaga gaggcttgaa gttttgaatc taaaaggatg 3660

ctcgaatttg ggtggcctgc cagtagggat tggacagcta actcatcttc agaggctgca 3720

tttgtttgtt ataggtggca gtagcgagca tgcaagaata tcagggcttc gaaacctcat 3780

cttgttaacc gacaatgatc tggaaataaa aaatattaaa tatgtagagg atccagatga 3840

tgcagaaaag gcctccctga aggaaaagag tggtatactc aatttgacat tggattggtc 3900

ttcaaatggt gcagaagggt gctcggatag tctggaagaa gagccattat tagatatgga 3960

aaaggagctg cgtgtgctca atggtcttga accaccatca caaattaaga agctgaatat 4020

ctataattac aaaggtaagc atttctcacg ttggatgatg aagcaaagaa aatcctcttg 4080

ttcagatagc ttgttagagc aaattgatcc acctcatttc actcaactga ctaaacttgt 4140

attggaacaa tttccaaacc tggagaatct gcaaggactt gcacggttgc cttcattgaa 4200

tacttttgta ttgaaaggaa tgcctaattt ggtggagttg tggacaagct cacctgcatt 4260

agtaagtggg gaggaaggaa ggccctgttg tattgatcac gaacaagttg agcactgctt 4320

ccctttccta tccactctag tcatgagtga ttgtcctaag ctgaagtaat gcccatgttt 4380

cccaccatat ttggatagct tgacactgga acgaagcaac gagcagctgc tgtctcaagt 4440

tagcttctct catccgctcc atcctcttga caaggaatcc tcatccatct gctttagtgt 4500

ggattcccct cacctcaaga aattagaact aggaggaatg gctggaccat cgtcaggttg 4560

ggaagtgctg cagaacctca ccgggctcga atccttgtca atttattcca gtgacctaag 4620

gcaattaggg gagatcatcc ggtgcctcac ctccctccag tatctttgta tctcgggttg 4680

cccagttctt gccatgctgc cagagtggct gggggggttc cgctctctgc aaactctagt 4740

tctgaaagaa atcccactat tggctagcct ccgcaagtcc atcatgctcc ttacatcccc 4800

ggagaagctg gctattgttg aatgcgacaa tctaaaggag ttgcctgagg tggtaaatca 4860

tcccacctcc ctcgaggaat tggatatttc tagttgcaga aatctgagcc agttgcctga 4920

gggaattcag catcttacca accttgaaga cctaagtata caggactgtc ttgccctcca 4980

caagctgcca gaaggactgg ggatgttagg ttctctagag gaccttatga tcaatatctt 5040

gcctgtcctc acgacccttc cggagtccat gcaggggctc acctcactcc ggcacatcaa 5100

cttgatgagc tgccctatgc tcaccgtgct gcctgagtca ctcggacaac tctccgcgct 5160

ccgatcgctt tatatgcaaa gctgcactgg ccttcgatcc ttgcccagct ccatacaaca 5220

ccttacttct cttcagcatt tggtgattag ttacaaccct accttgagta ggcattacaa 5280

aaatagggtc gggaaggact ggcacatcat ctcccatatt cctgttgtgg agatacgaga 5340

ttagggagca tgctgggcac tagcaacaaa taaacgagca tggcaaggtg gtgacatact 5400

acttctgtgg ctctgtgcaa ctatgcctta ttattgcacg tcccttttgt atcctcaata 5460

cttgttacta atttcccaac ccatcttttt tctataattg ttccagaacc ccctgctggt 5520

ggagtcgtgg agcagagcat atgtgttcat gaaccagctt tggagatgat cactctcaag 5580

ttttgaatct tgacatgcaa ggaaggatgg ataagataca cactcttttg ttttagttat 5640

cttacaagtg agaatctcag ggcacaaatt tgctgtttga gaaaaatgct catttggtac 5700

ttccactatc ttttccagga ctgcaatctt tttttcttgc cattcgaatt tactgaacaa 5760

attaattaag catgcttgcg caagcaaaca ccattctatt ttattctcat ggatttcctt 5820

gggaatattc cattgaaaaa aaaaactctt ttccttggtc cacaaaaaaa aaaaaacaca 5880

cactgtttta taggtttcct tttcagacat gttcattgtg ttcacagttt cacaccattt 5940

tttttgtgag acggtacaaa atgcaaattt tcaggaagat gcctgcaggc tggtcaggtt 6000

acaatgtggc attgctgctt tacggtcagc aggctagcat gtttcctgta ttgtgtaagg 6060

agtattctga aagatgttcc tgagactatg gtggctgtaa aatactgaga gtgttccatc 6120

atactcccta tataccaaaa agaatccaac cctagagata aatctggaca tacactatat 6180

acagatttaa cctcaggatt aggtttttta tgggacaaag gaagtaactt taagtagtga 6240

aaaagtttgg caatcattct tggtttgata agaatcttga gcacatcaga ttttgcattt 6300

gggatgcaaa ataatagatg aggacttact ctagacatta atcatttgca gggtcggggt 6360

atttgaatta gcctgatgat caacataaga aaatctgcat gagatgaaca gaatgtactc 6420

gacaaagaac tgggctcttc ctgggaatac atctgttttg ttattttgat attaacacta 6480

gcctggagtt tgtacttgtg aagagaagcc agggagttga tatacgaact tatcatttaa 6540

agatgcactg ttcatatgtt ccttgtttac cgaacattct gcagtaagct tcttttacca 6600

gggacttgat tgatttgctc gagaaaatca accaagaatt gctatgctta taggaccatg 6660

aatttgatcg ttcgacaaac acaagtgttt tacgagtgcc aggcacatct tttggtcgaa 6720

gaatacaact cagaaaattg atttgcagtc ggcatatcgt tcaattcaac atgcagtctc 6780

gcagttggca tgctacgctt ttgtacttgt aatattggca tctttggtac ttgataaatg 6840

cattttaagt tgatcacggg gctaccatta tgtgcgctcc ctctattaat taatatccag 6900

agttgttgct ctgtgacatt cctatcacac aacatatgta tgcgcttatt tgcaagggag 6960

actgtcgatc gtaacatatg cacttcatgt cgggctttgg gagcactgat aagctagttc 7020

ttcttgagct gtcttgatgc atcaagcagc acatggaggt cgctaattaa ttggtctgtt 7080

gggctgcagc tgatacttga gccgtctcag ctgcttcctg ctcagctcca ctggcgtcac 7140

cgggcacatc cacgaccatc ttggaaacac ggaacatggc caggtggatg tcgcctgcaa 7200

tacaatgcaa aagaggaaca agcacagact gatcacaacg aagaagagaa ggcaagagat 7260

gctgcgagtt gtatgcagta tgaagagagt gaggtgagct aatttgtagc agtaataact 7320

aataagtagt agtaccaaaa atgaggaggc taccggggtc gatgggagtg gggaaacccg 7380

gggtgaggcg cctctcgttg atgtaggtgc cattcgtgct ctccaggtca gttaccaaca 7440

ggctcccctc tttcttctcc aaccgtgcgt gcgtcccaga aactgcttct tttcatgcca 7500

ttccattcca tttcatttca tttcatttgc agcaacttag taagctacat atgtctgatg 7560

gattatggag agagcaatgc aagtgaatga atgtgctgca tatatatatg ctactacctg 7620

ttgcgacaga caggacgatg tcagcctgtt caggaacacg acccaccgtc accgccgcct 7680

cctgcacaca caagacaaca cattcattca tccaagagaa cacccggctg cagctgagct 7740

agcacggtta catggcatgg atatccagag aatttctggg aaggtagaaa caaagagaga 7800

catacggata ctatctggaa gccgccgggt cgtgcgacgc ggtacccgat atgacgccaa 7860

tcgccgtctc ctgcaattca acaacagcag gttctgtttg ttcaggtgga cagcaataaa 7920

ctcacgaata ttgctgctgt gttctgtttt gcaatttttt cttcagacca gctagttaaa 7980

gttggtaaga tgacgatact atgattaagc cggcaggcag gcacaagcat gagccagttc 8040

acaagggata tcgacattgc attacaagta tcattttcac aagtattgaa agcagactaa 8100

cctgcaggct caagaaccca tctttctgaa gtggcggaag aagacgcgct gtcagtgcca 8160

ctggaagagc acctcagcga caaaatggaa atgcttcttc tgccactccc gttgctgttt 8220

ctg 8223

<210> 5

<211> 1180

<212> PRT

＜213〉Oryza paddy rice (Orysa sativa L.)

<400> 5

Met Ala Gln Arg Cys Ser Arg Ala Val Val Val Phe His Leu His

1 5 10 15

Asp Glu Val Trp Ile Trp Ala Lys Glu Lys Leu Pro Ser Phe Lys

20 25 30

His Met Gly Met Glu Ala Ala Val Val Ser Gly Leu Phe Lys Ile

35 40 45

Val Gly Asp Lys Leu Ala Pro Leu Val Ile Arg Glu Tyr Ser Ser

50 55 60

Leu Met Gly Val Thr Lys Asp Leu Gln Glu Leu Gln Asp Leu Val

65 70 75

Glu Glu Ile Lys Ser Gly Leu Gln Ser Glu Lys His Asp Thr Asp

80 85 90

Asn Asp Arg Asp Lys Tyr Ala Val Leu Lys Tyr Met Arg Thr Lys

95 100 105

Pro Lys Leu Ala Met Phe Gln Leu Lys Met Ala Asn Lys Ile Lys

110 115 120

Thr Ile Lys Asn Arg Phe Ala Ala Ile Val Glu Gln Arg Gly Asp

125 130 135

Ile Ile Ser Glu Pro Ser Leu Leu Gly Asn Val Asp Asp Ser Lys

140 145 150

Ile His Val Arg Asp Glu Glu Lys His Lys Ile Ile Arg Lys Leu

155 160 165

Ile Asp Asp Gln Gln Lys Ile Ser Ile Val Ser Ile Val Gly Leu

170 175 180

Gly Gly Thr Gly Lys Thr Thr Met Ala Thr His Ile Cys His Asp

185 190 195

Asn Lys Ile Lys Glu His Phe Glu Gly Ser Ile Phe Trp Val His

200 205 210

Val Ser Gln Glu Phe Asp Asn Asn Lys Leu Val Gly Lys Leu Tyr

215 220 225

Glu Ala Ile Leu Lys Lys Thr Ser Tyr Leu Arg Thr Asp Gln Gln

230 235 240

Met Val Glu Ala Ile Ser Asn Glu Leu Asn Gly Asn Lys Phe Leu

245 250 255

Leu Val Leu Asp Asp Ala Trp His Lys Asn Gln Tyr Asp Trp Glu

260 265 270

Arg Phe Met Leu Tyr Leu Lys Ser Gly Ser Pro Gly Ser Arg Ile

275 280 285

Leu Leu Thr Thr Arg Asp Leu Gly Val Ala Glu Ala Val Glu Ser

290 295 300

Thr Cys Thr Tyr Thr Leu Ala Phe Leu Ser Asp Glu Asp Ser Trp

305 310 315

Asn Leu Phe Gln Gln Ser Leu Arg Leu Ala Ala Lys Gly Leu Pro

320 325 330

Ser Glu Phe Val Glu Ile Gly Arg Glu Ile Ile Lys Lys Cys Gly

335 340 345

Gly Val Pro Leu Ala Ile Lys Ile Leu Ala Gly Val Leu Arg Asn

350 355 360

Lys Lys Thr Val Asp Ala Trp Cys Ala Leu Arg Asp Ser Asn Met

365 370 375

Trp Asn Val Asp Asp Ile Glu Asp Arg Val Phe Ala Ser Leu Arg

380 385 390

Leu Ser Tyr Phe His Leu Pro Asp His Leu Lys Gln Cys Phe Val

395 400 405

Tyr Cys Ser Ile Phe Pro Lys Gly Tyr Lys Ile Tyr Lys His Gln

410 415 420

Leu Ile Gly Glu Trp Ile Ala Asn Gly Phe Ile Asn Pro Met Asn

425 430 435

Glu Ile Glu Gln Val Glu Asp Val Ala Asn Asp Cys Phe Asp Ser

440 445 450

Leu Leu Lys Val His Phe Leu Gln Asp Leu Gly Val Asp Glu Tyr

455 460 465

Asp Glu Met Glu Ile Cys Lys Met His Asp Leu Val His Asp Leu

470 475 480

Ile Arg Gln Ile Leu Gln Gly Glu Met Val Ser His Ser Gln Asn

485 490 495

Ala Thr Ile Gly Asn Ser Gln Lys Cys Arg Tyr Leu Ser Leu Ala

500 505 510

Ser Cys Asn Glu Asn Ile Glu Val Lys Leu Phe Ser Lys Val His

515 520 525

Ala Ile Tyr Ile Ser Gly Asp Asn Phe Ala Leu Asn Lys Pro Ile

530 535 540

Lys Lys Arg Ala Leu Ser Thr Leu Pro Glu Ser Ile Gly Lys Leu

545 550 555

Lys Lys Leu Arg Thr Leu Glu Leu Ser Cys Leu Leu Asp Leu Glu

560 565 570

Ser Leu Pro Gln Ser Ile Gly Asp Cys His Asn Leu Gln Ser Phe

575 580 585

Leu Leu Arg Gly Ser Gly Ile Arg Glu Ile Pro Asn Ser Ile Cys

590 595 600

Lys Ile Lys Lys Leu Arg Val Leu Asn Ile Met His Cys Arg Ser

605 610 615

Leu Arg Gln Gln Trp Ser Glu Phe Phe Gly Thr Leu Cys Asn Leu

620 625 630

Gln Ser Ile Asn Leu Ala Gln Ile Glu Gly Ile His Asn Leu Phe

635 640 645

Ser Ser Phe Ala Cys His Lys Leu Arg Thr Leu Thr Leu Ser Gly

650 655 660

Thr Glu Ile Thr Arg Leu Pro Gln Cys Leu Thr Leu Val Ser Thr

665 670 675

Leu Glu Tyr Ile Asp Leu Gln Asn Cys Trp Gly Leu Leu Glu Leu

680 685 690

Ser Glu Gly Ile Gly Asn Leu Glu Arg Leu Glu Val Leu Asn Leu

695 700 705

Lys Gly Cys Ser Asn Leu Gly Gly Leu Pro Val Gly Ile Gly Gln

710 715 720

Leu Thr His Leu Gln Arg Leu His Leu Phe Val Ile Gly Gly Ser

725 730 735

Ser Glu His Ala Arg Ile Ser Gly Leu Arg Asn Leu Ile Leu Leu

740 745 750

Thr Asp Asn Asp Leu Glu Ile Lys Asn Ile Lys Tyr Val Glu Asp

755 760 765

Pro Asp Asp Ala Glu Lys Ala Ser Leu Lys Glu Lys Ser Gly Ile

770 775 780

Leu Asn Leu Thr Leu Asp Trp Ser Ser Asn Gly Ala Glu Gly Cys

785 790 795

Ser Asp Ser Leu Glu Glu Glu Pro Leu Leu Asp Met Glu Lys Glu

800 805 810

Leu Arg Val Leu Asn Gly Leu Glu Pro Pro Ser Gln Ile Lys Lys

815 820 825

Leu Asn Ile Tyr Asn Tyr Lys Asp Ser Leu Leu Glu Gln Ile Asp

830 835 840

Pro Pro His Phe Thr Gln Leu Thr Lys Leu Val Leu Glu Gln Phe

845 850 855

Pro Asn Leu Glu Asn Leu Gln Gly Leu Ala Arg Leu Pro Ser Leu

860 865 870

Asn Thr Phe Val Leu Lys Gly Met Pro Asn Leu Val Glu Leu Trp

875 880 885

Thr Ser Ser Pro Ala Leu Glu Ser Ser Ser Ile Cys Phe Ser Val

890 895 900

Asp Ser Pro His Leu Lys Lys Leu Glu Leu Gly Gly Met Ala Gly

905 910 915

Pro Ser Ser Gly Trp Glu Val Leu Gln Asn Leu Thr Gly Leu Glu

920 925 930

Ser Leu Ser Ile Tyr Ser Ser Asp Leu Arg Gln Leu Gly Glu Ile

935 940 945

Ile Arg Cys Leu Thr Ser Leu Gln Tyr Leu Cys Ile Ser Gly Cys

950 955 960

Pro Val Leu Ala Met Leu Pro Glu Trp Leu Gly Gly Phe Arg Ser

965 970 975

Leu Gln Thr Leu Val Leu Lys Glu Ile Pro Leu Leu Ala Ser Leu

980 985 990

Arg Lys Ser Ile Met Leu Leu Thr Ser Pro Glu Lys Leu Ala Ile

995 1000 1005

Val Glu Cys Asp Asn Leu Lys Glu Leu Pro Glu Val Val Asn His

1010 1015 1020

Pro Thr Ser Leu Glu Glu Leu Asp Ile Ser Ser Cys Arg Asn Leu

1025 1030 1035

Ser Gln Leu Pro Glu Gly Ile Gln His Leu Thr Asn Leu Glu Asp

1040 1045 1050

Leu Ser Ile Gln Asp Cys Leu Ala Leu His Lys Leu Pro Glu Gly

1055 1060 1065

Leu Gly Met Leu Gly Ser Leu Glu Asp Leu Met Ile Asn Ile Leu

1070 1075 1080

Pro Val Leu Thr Thr Leu Pro Glu Ser Met Gln Gly Leu Thr Ser

1085 1090 1095

Leu Arg His Ile Asn Leu Met Ser Cys Pro Met Leu Thr Val Leu

1100 1105 1110

Pro Glu Ser Leu Gly Gln Leu Ser Ala Leu Arg Ser Leu Tyr Met

1115 1120 1125

Gln Ser Cys Thr Gly Leu Arg Ser Leu Pro Ser Ser Ile Gln His

1130 1135 1140

Leu Thr Ser Leu Gln His Leu Val Ile Ser Tyr Asn Pro Thr Leu

1145 1150 1155

Ser Arg His Tyr Lys Asn Arg Val Gly Lys Asp Trp His Ile Ile

1160 1165 1170

Ser His Ile Pro Val Val Glu Ile Arg Asp

1175 1180

<210> 6

<211> 1786

<212> DNA

＜213〉Oryza paddy rice (Orysa sativa L.)

<400> 6

tgagacaaag gagtggcacg ttggatgacg gtacatctcc atttcctaac ctagttgaat 60

tgcatatgag gaatctaccg aaagtttgtg gagtggcacg tgagatgacg gtacatctcc 120

atttcctggt taaattgtgc atttctcgtt gtagtaatat gagggaggga gttgaaggca 180

ttgaacacct ctcctccctc caaagggaaa tttaactatt tgtcactctt gtaacatggc 240

aattaacgat ttgccacacc atgtctataa catgtgggcc ctcatgtgtc tatgatatgt 300

ggacctaatg gcaaatcgtc aagcaccact cgtaagagtg gcaaatagtt aaaagccccc 360

cctccaaaac ccgtggatac aagaaagcca tgccctcgag atgctctcta aaatacatcg 420

tgctccatca tgggctcacc tcactccaac acctccaact tttgtttcct acccgtgtgc 480

tcatcatgct actagaatca ctcgggcaaa ctctctacac aaccgctttc tagccaaaga 540

taccttggcc tttagtcctt gactcctttc ccaactccat acaatgcctt acttctttat 600

ataatttgct gattagttac atccttgctt tgagtaggtg ttacaaggtc aggggaagga 660

ctagcacatc ctctcccaca tttgtcagat gtagatatta ggtaattgga aattaggata 720

tatattagag ataagacaat gaggtcacaa cattccgctg atcgtggtcg cattaaggtg 780

tccatacatg tatgttattt tattacattg ttatcactaa tgtcatcttt cattgcatgt 840

atgtcaggcc caaacttcat ctcattttcc accctcctgg tggaacagag cacacatgca 900

tatggtgacc agtgaaatca atgaagcagc taatttgctg ctactcatta ttttgaccgc 960

tgatacatga taagtggacg agattgtcac tcacctgatt ttgaagttag ttacttcctc 1020

tgttaaaaaa atataagcgc atatgcgact taaaatttgt actacaataa aaacacttaa 1080

ttgtatacca attgagattt cccgcagtac cgctcattga catatgggct tgagggtctg 1140

taaggcccac atgtcagtgt gtaatactat actgcagatg atctgtatcc gttgtataca 1200

cgttgcgtca ttttgggtct tgcatgagtt taattgagcc tatggtctgc gcatgaaggc 1260

tgcactatag caataaaaag agggcaggaa agttgcacta caatcaccaa acatggcaag 1320

cccgctccat tgataaatag tccattggtg caagcctatt tatgttaaaa accgatttga 1380

agaaagaata tttacatttt aacctctttt aaaaaattta ttttataaat aggtccccga 1440

tgataatctc tttagtggca ctgatcctct acatctcgac tgttgggggg tggaggggat 1500

aagggtgtca atactaatat cgttggtgtt gagacattgt acgttggcga gggtcactaa 1560

catcgagata atggtctaat tctgaaataa ttttgtatag ggttaattta caaagtaagt 1620

tctctaaaac tatgctggta gaatgtaaaa taacccaggt tccaatgtta atcgttcggc 1680

caccgcttgc gcgaccgttg ctgccggttg ccgccgccag gactcacggc ttgctcccct 1740

cccttcgcct ccggctccta tcccatccgc atactgccgc ggacgc 1786

<210> 7

<211> 5519

<212> DNA

＜213〉Oryza paddy rice (Orysa sativa L.)

<400> 7

atggagggcg ccattgtgag cttgaccgag ggcgccgtgc gggggctcct ccggaagctc 60

gccggcgtgc tcgcgcagga gagctcgccg gcgcagcgcg tccacggcga ggtgcagtac 120

atcaaggacg agctggagag catgaacgcc ttcctccgca gcgtctccac gtcgccggag 180

gacgccgccg gccacgacga ccaggtgagg gtctggatga agcaggtcag ggagatcgcc 240

tacgacgccg aggactgcat cgacgtgttc gtccgcggcc gcagccaccc cgccgccgcc 300

gccggagacg aaggtcgcct cgtggcctca ctccgccgct tcgtccgcct actggccggc 360

gcgctaggag taggaggagg agaccgctcc gtcgcggccc agctccggga actgaaggcc 420

cgcgcccgcg acgccggcga gcggcgcacg gggtacgggg tgtcgctggc ggcggcggcg 480

gtcaggggcg gcggcgggtc gtcgtcgtcc gggcggctcg accccaggct gcacgcgctg 540

ttcgcggagg aggcgcagct ggtggggatc gacgggccga gggaggagct cgtggggtgg 600

gtgatggagg aggagccgcg gctgcgggtg ctcgccgtcg tcgggttcgg cgggctcggc 660

aagaccacgc tggcgaggat ggtgtgcggg agcccccggg tgaagggcgc cgccgacttc 720

cagtgctcgc cgccgctcgt cgtcgtgtcg cagacgttca gcatcacggc gctctcccag 780

cacttgctca gggagctcat ccagcgcccg cgcaaggcaa tggctgccgt cgccgccgcc 840

ggcggcggcg gcggcgacct tgtcgcgtac gatgcattgc aggggatgga gaggtgggag 900

acggcagcgc tcgccagcaa agctgaggga atacctgcaa gacaaaaggt tagtactccc 960

ttcatttcag attataatca gattataagt tgttttaact ttagttaaaa tcaaattgct 1020

ttaaatttga ttaagtttgt aaaaaaaaaa agtaatattt tcgacctaaa ataaatatat 1080

tatggaaata tattcaacta ttgatttaat gaaactgatt tggtgttgta aatattacta 1140

tatttgtcta taaacttatt caaacttgaa gtagtttgat tttgatcaaa gtcaaaaaag 1200

acttataatc tgaaacgcga ggagtatttc ctttccttgt tcaacttaat ttctcgagtt 1260

acaacaatgt agtactacta tactaaccag tattactata acaactaaga aaaagaacat 1320

tagtattact atactaagaa aaagaaaatg ttcgagtaac taagaacact acttcagcta 1380

agtttgactt tatagaaaaa ccgtagtaat accaaattag ttttattaaa tcaaacatta 1440

aatatatttt gatactatat ttgttttgtg ttcaaaatgt tgctacattt ttctataaaa 1500

ttgcttaaag ttaaaaatgt ttgacttaga aaaaccaaaa tgtcttataa tataggggga 1560

gaattaatga gttacagtaa cacaaaattg tttccaacaa tgacactctt ccattgaatt 1620

tatcctatta gttttattag gtaggtttga tgaagttcaa ccttctcctg ctcgggttat 1680

ttgttctcta ttagagaatc ataatctatt gattagttga accaaactcg attttactac 1740

tattcaactc agtcttgccg gttgtacaaa atttccttcc agtagcaggt agtagctaaa 1800

aaaacagatc atatatgtag ttaattattt tatttgttgc cgagtttgtt catatatgtg 1860

gaactattac attgtatagg tacattgtga tattggatga tatatggtct tcatcagcat 1920

gggagagcat caaatgcgca tttccagata ataaaaaggg tagcaggatc atagtaacca 1980

ccagaaacga ggatgtcgcc aacacctgct gctgccgccc ccaagatcgc atctacaaga 2040

tccaacgcct ctcagatgcg gcatcgcggg agctgttctt caagaggatc tttggcatgg 2100

cggatgccgg agcgccagac gacgatgagc tgaaacaagt ttcagattcc atcctgaaga 2160

aatgtggagg gctgcctcta gcaatagtga gcatcggtag cctcctcgca agcaagccga 2220

acaggagcaa agaagagtgg cagaaggttt gtgacaactt gggctctgaa cttgagtcaa 2280

atcctactct tgaaggcaca aagcaggtgc taactctgag ctacaatgat ctgccctacc 2340

atctgaaggc atgcttcttg tatttgagta tctttcctga aaatcatgtg atcaagaggg 2400

gacctttggt gagaatgtgg atagctgaag gttttgtcac ccagaggcat ggattgagca 2460

tggaacaggt cggtgaaaga tactttgatg agtttgtgag caggagcatg gttcatcttg 2520

tcagaatcga ttggagtggc aaggtgagaa gctgcaaggt tcatgatata atgcttgaag 2580

tcatcgtctc caagtcactt gaggaaaact ttgcatcctt cttctgtgac aatggaaccg 2640

aactggtttc gcatgacaaa atccgccgcc tctccatccg cagcagtagc tactcatcag 2700

ctcaaagaac aagcaacagt gtagctcatg ttcgcacctt cagaatgtca ccttcaattg 2760

acaatatccc attcttcttt ccacaattgc ggttgttaag agtgctggac atgcaaggca 2820

gccgttgcat gagtaacaag aatttggact gcatctgcag acttttccag ctcaaatacc 2880

taagcctcag aaacaccagt gtgtccatac ttccgaggtt aattgggaat ctgaatcacc 2940

tggagacact ggatataaga gagacactta tcaagaagct gcctagtagt gcagctaacc 3000

tgacttgcct aaagcatttg ttagcagggc acaaagagca acttacaagg acatccagtg 3060

tgaagttttt gaggccatcc tcaggtctaa agatgagcca tggagtgatc agaaacatgg 3120

caaagctgca atcacttgtc catgtagaga taaaagaaca tccatcagtg ttccaggaga 3180

tcgccttgct gcaaaatcta cggaagctca gtgttctctt ttatggtata gaggtaaact 3240

ggaagccctt tctggagttg ttgaatatgc tgagtggctc tgtgcgatca ctctccattg 3300

acatcttcga cgcacaagga aacatctcta tctcatcact ggagatgctt tcttccttag 3360

tgtcacctcc cattttcatc actagcttca gcctgactgg caagcttggt agtctacctc 3420

cttgggtagc atcacttaga agcgtctcga ggcttactct tcgcaggagc caactccgtg 3480

ctgatgccat acatgtcctt ggaggcctgc aaaatctgct ctgcctgaaa ctctatcaca 3540

aatcatatgc tgatgatcgc ctcgtttttc cacagggagg atttgccagg gtcaagcttc 3600

tgatcgatga taacctggtg aaccttgaaa agttgcattt caatgaggga tcgatgccta 3660

atcttgagag gctgacattg tcattcctga gagaacccaa ggatggcatc tcaggtctga 3720

acaacctcct gaaactgaag gaggtagagt tctttggcaa catcgtatca tctgtggtga 3780

gcaaagtggt gtcatgtgtg aaggatcatc caaatcatcc gagagttgta ggtgacaaat 3840

ggaacattgt gacggtgtac aattgaggtg taaaacagta aaagaagcat ttcatctgaa 3900

gcttgcgttt gcttcagtgt tctgcaggtt gtggctaggc ctcccctgct agtttaaact 3960

tatcaaatgt actcctattt cataagcaca acattttgct tgtcaactac tttgttaggt 4020

ttcttgtgat tgaacctgcc tattcgagtt taaattttag gcttgacacg agtgcttgta 4080

tttatggata tttattcttt cagggataga tgatataccc gttaacaatg agacgtttgt 4140

ggagactttg tcattatcaa gatatacatg cctggtcttt caggggtgct tataaagaat 4200

tagagatatg ctgtgtgttt ataaggataa gtgcccctac caataggtgg acccttgtca 4260

ctgggctccg tgccatagcg tccaaaattg gtgggaaatg ctagccaata caaatgaggt 4320

gtccaagaaa agcctgcgga ccctaattct tttaatagtt tgggaaatct ggaagtaaaa 4380

aaatcagcag gttttcgaac gaaaggagat tgcgacctta gccttatggt aaaaataaaa 4440

gaggaggcag gaacttgggc tatggcggga gcaaggagga tacgggattt tctactaaac 4500

cgtatctagg ccttatcagt tttgtatagt tttatcccta ggggttgtac ttttccttct 4560

attttaatga aatagtatag tctcgtgttg attcctttaa aaagataagt acacgcacgt 4620

ttctagggct acgtttgtac tgtatttttt taaaaaaaaa tgttctaaaa aaattaccac 4680

cgtcattttc ctttcaccta aggatagcat atgagcatga cttaaacaaa agtagaaaac 4740

acacatccgt gcttggaagg cgtatttctg tatcacgtgg gggaggcaca cgcctatgaa 4800

gttcggtgac gccttatgaa tgtacgcgtc cttactgtct ctttcggaaa agagagagag 4860

agagagagag agccgcagcc gcagccctcc gcgccggcga tggcggcggc ggaggacgtg 4920

ccgggtagag attccggccg gatgcgcatg cgcatgcgca gaagattcaa gggagccggg 4980

tgaagcacgg atcgtggtgg tggctaatcg aagagcgcgc gcgggtgggc ggctctgtgc 5040

ttcatgtgtt cgatgaaatg cgtcacccag ctagtctacg agaagggcct gactaggtag 5100

cggcggcgag cagctggccc tgaatgtttc gcaagggaga gatcagagat gtcgcaggtg 5160

taacaaccaa gctctcctag gtcttgctcg actcggcccc cgctgaagtg cttcacaatc 5220

caaggtgctt gatttgtctt cttccatgtc aactttcttt tgcgctctca aatggaacca 5280

ttgagctcct ggagtctctt tcacaggact ggatatgctg tggctcggtt tcatctttta 5340

tgcaaatctt taaggggaaa aagaagagag tcaaacacta cagtcaagat gatgctggta 5400

actgtgcctg tttttccaat ggttgcttga ttgcctttct gcatttatgt cgacatcgcc 5460

atttctttca aggtgactag tactaggtta ttatacaaaa ttacaagggg aacacgcaa 5519

<210> 8

<211> 989

<212> PRT

＜213〉Oryza paddy rice (Orysa sativa L.)

<400> 8

Met Glu Gly Ala Ile Val Ser Leu Thr Glu Gly Ala Val Arg Gly

1 5 10 15

Leu Leu Arg Lys Leu Ala Gly Val Leu Ala Gln Glu Ser Ser Pro

20 25 30

Ala Gln Arg Val His Gly Glu Val Gln Tyr Ile Lys Asp Glu Leu

35 40 45

Glu Ser Met Asn Ala Phe Leu Arg Ser Val Ser Thr Ser Pro Glu

50 55 60

Asp Ala Ala Gly His Asp Asp Gln Val Arg Val Trp Met Lys Gln

65 70 75

Val Arg Glu Ile Ala Tyr Asp Ala Glu Asp Cys Ile Asp Val Phe

80 85 90

Val Arg Gly Arg Ser His Pro Ala Ala Ala Ala Gly Asp Glu Gly

95 100 105

Arg Leu Val Ala Ser Leu Arg Arg Phe Val Arg Leu Leu Ala Gly

110 115 120

Ala Leu Gly Val Gly Gly Gly Asp Arg Ser Val Ala Ala Gln Leu

125 130 135

Arg Glu Leu Lys Ala Arg Ala Arg Asp Ala Gly Glu Arg Arg Thr

140 145 150

Gly Tyr Gly Val Ser Leu Ala Ala Ala Ala Val Arg Gly Gly Gly

155 160 165

Gly Ser Ser Ser Ser Gly Arg Leu Asp Pro Arg Leu His Ala Leu

170 175 180

Phe Ala Glu Glu Ala Gln Leu Val Gly Ile Asp Gly Pro Arg Glu

185 190 195

Glu Leu Val Gly Trp Val Met Glu Glu Glu Pro Arg Leu Arg Val

200 205 210

Leu Ala Val Val Gly Phe Gly Gly Leu Gly Lys Thr Thr Leu Ala

215 220 225

Arg Met Val Cys Gly Ser Pro Arg Val Lys Gly Ala Ala Asp Phe

230 235 240

Gln Cys Ser Pro Pro Leu Val Val Val Ser Gln Thr Phe Ser Ile

245 250 255

Thr Ala Leu Ser Gln His Leu Leu Arg Glu Leu Ile Gln Arg Pro

260 265 270

Arg Lys Ala Met Ala Ala Val Ala Ala Ala Gly Gly Gly Gly Gly

275 280 285

Asp Leu Val Ala Tyr Asp Ala Leu Gln Gly Met Glu Arg Trp Glu

290 295 300

Thr Ala Ala Leu Ala Ser Lys Ala Glu Gly Ile Pro Ala Arg Gln

305 310 315

Lys Phe Val His Ile Cys Gly Thr Ile Thr Leu Tyr Arg Tyr Ile

320 325 330

Val Ile Leu Asp Asp Ile Trp Ser Ser Ser Ala Trp Glu Ser Ile

335 340 345

Lys Cys Ala Phe Pro Asp Asn Lys Lys Gly Ser Arg Ile Ile Val

350 355 360

Thr Thr Arg Asn Glu Asp Val Ala Asn Thr Cys Cys Cys Arg Pro

365 370 375

Gln Asp Arg Ile Tyr Lys Ile Gln Arg Leu Ser Asp Ala Ala Ser

380 385 390

Arg Glu Leu Phe Phe Lys Arg Ile Phe Gly Met Ala Asp Ala Gly

395 400 405

Ala Pro Asp Asp Asp Glu Leu Lys Gln Val Ser Asp Ser Ile Leu

410 415 420

Lys Lys Cys Gly Gly Leu Pro Leu Ala Ile Val Ser Ile Gly Ser

425 430 435

Leu Leu Ala Ser Lys Pro Asn Arg Ser Lys Glu Glu Trp Gln Lys

440 445 450

Val Cys Asp Asn Leu Gly Ser Glu Leu Glu Ser Asn Pro Thr Leu

455 460 465

Glu Gly Thr Lys Gln Val Leu Thr Leu Ser Tyr Asn Asp Leu Pro

470 475 480

Tyr His Leu Lys Ala Cys Phe Leu Tyr Leu Ser Ile Phe Pro Glu

485 490 495

Asn His Val Ile Lys Arg Gly Pro Leu Val Arg Met Trp Ile Ala

500 505 510

Glu Gly Phe Val Thr Gln Arg His Gly Leu Ser Met Glu Gln Val

515 520 525

Gly Glu Arg Tyr Phe Asp Glu Phe Val Ser Arg Ser Met Val His

530 535 540

Leu Val Arg Ile Asp Trp Ser Gly Lys Val Arg Ser Cys Lys Val

545 550 555

His Asp Ile Met Leu Glu Val Ile Val Ser Lys Ser Leu Glu Glu

560 565 570

Asn Phe Ala Ser Phe Phe Cys Asp Asn Gly Thr Glu Leu Val Ser

575 580 585

His Asp Lys Ile Arg Arg Leu Ser Ile Arg Ser Ser Ser Tyr Ser

590 595 600

Ser Ala Gln Arg Thr Ser Asn Ser Val Ala His Val Arg Thr Phe

605 610 615

Arg Met Ser Pro Ser Ile Asp Asn Ile Pro Phe Phe Phe Pro Gln

620 625 630

Leu Arg Leu Leu Arg Val Leu Asp Met Gln Gly Ser Arg Cys Met

635 640 645

Ser Asn Lys Asn Leu Asp Cys Ile Cys Arg Leu Phe Gln Leu Lys

650 655 660

Tyr Leu Ser Leu Arg Asn Thr Ser Val Ser Ile Leu Pro Arg Leu

665 670 675

Ile Gly Asn Leu Asn His Leu Glu Thr Leu Asp Ile Arg Glu Thr

680 685 690

Leu Ile Lys Lys Leu Pro Ser Ser Ala Ala Asn Leu Thr Cys Leu

695 700 705

Lys His Leu Leu Ala Gly His Lys Glu Gln Leu Thr Arg Thr Ser

710 715 720

Ser Val Lys Phe Leu Arg Pro Ser Ser Gly Leu Lys Met Ser His

725 730 735

Gly Val Ile Arg Asn Met Ala Lys Leu Gln Ser Leu Val His Val

740 745 750

Glu Ile Lys Glu His Pro Ser Val Phe Gln Glu Ile Ala Leu Leu

755 760 765

Gln Asn Leu Arg Lys Leu Ser Val Leu Phe Tyr Gly Ile Glu Val

770 775 780

Asn Trp Lys Pro Phe Leu Glu Leu Leu Asn Met Leu Ser Gly Ser

785 790 795

Val Arg Ser Leu Ser Ile Asp Ile Phe Asp Ala Gln Gly Asn Ile

800 805 810

Ser Ile Ser Ser Leu Glu Met Leu Ser Ser Leu Val Ser Pro Pro

815 820 825

Ile Phe Ile Thr Ser Phe Ser Leu Thr Gly Lys Leu Gly Ser Leu

830 835 840

Pro Pro Trp Val Ala Ser Leu Arg Ser Val Ser Arg Leu Thr Leu

845 850 855

Arg Arg Ser Gln Leu Arg Ala Asp Ala Ile His Val Leu Gly Gly

860 865 870

Leu Gln Asn Leu Leu Cys Leu Lys Leu Tyr His Lys Ser Tyr Ala

875 880 885

Asp Asp Arg Leu Val Phe Pro Gln Gly Gly Phe Ala Arg Val Lys

890 895 900

Leu Leu Ile Asp Asp Asn Leu Val Asn Leu Glu Lys Leu His Phe

905 910 915

Asn Glu Gly Ser Met Pro Asn Leu Glu Arg Leu Thr Leu Ser Phe

920 925 930

Leu Arg Glu Pro Lys Asp Gly Ile Ser Gly Leu Asn Asn Leu Leu

935 940 945

Lys Leu Lys Glu Val Glu Phe Phe Gly Asn Ile Val Ser Ser Val

950 955 960

Val Ser Lys Val Val Ser Cys Val Lys Asp His Pro Asn His Pro

965 970 975

Arg Val Val Gly Asp Lys Trp Asn Ile Val Thr Val Tyr Asn

980 985

<210> 9

<211> 1573

<212> DNA

＜213〉Oryza paddy rice (Orysa sativa L.)

<400> 9

cctgcccaat ctgctctgtc tcaagctcta ccaaaggtcc tacgctgatg accatatatt 60

ttttgctcat ggtaattttt tgaagctcag aatgttggtt attgataata tggagaacat 120

tcgcaatgtg cacttcgaga agggctcagt ccctaacctt gagtggctga caatagcatt 180

cctgcaagaa cccaaggatg ggattacagg tctggagaat ctgctgaagc tgaaggagat 240

agagttcttt ggcgacatca tattatccat ggtgactaaa gtggcatcct gcatgaaggc 300

acatccaaat cgcccgagag tgataggtga caaatggaac aatgtgacgg agtacgctta 360

aggagcaagc tctaaacaac attgcttcta gaggcctgtt tggttttggt gttcaggttc 420

agctagctag ttgggcaatg cttgtacctt catctcgaaa tataagtatt tctagctctg 480

aatctagtca agcttgtcta ggcttaaagc cagaaatgct tgcattttgg gaagtggttt 540

gcattgctta tttcaagcaa tgcagaaagt gtagtgtaac cagctggaca gtatgtattg 600

atagtttttc agggtgtgtt cctaagaaga caagctgagt tggtttgtca agagtagttg 660

aaagtacaac tctgtattgt atttaccttg cttgtaagag cggggaatcc tctcgattcc 720

attcgtttct ttgtgaaatt gtttacatgg ttaggttgtc ttcggatggc atatttttgc 780

gcaactctct ctccacacag tattgcaatg atatagggtg atggtcacta ctggtactat 840

tatcgttttt aaatatagga agtcgttgac ttttcaccac ggtgtccgac cattcatttg 900

atatttgtca tgacttattt tgtggttaaa agtaatttaa gcatcatatt tttacatatt 960

tgtataaaaa atattaatat gagaggatag ccaaatgtta tactacctct gtcccatatt 1020

aagtttatct tttagctcat tttatttgtc ccaagataag ttcattttta gagtaatcat 1080

tgcatcggaa tttgtgaaag taaggaataa ttgtactctc tctatccggt aatataaaac 1140

gtggtcaaag ttggcacagt cttcaaaact aatctttagc ttctaatttc tcatattcta 1200

taagttttgt agcatatgaa agtaaattaa atctcaatct aataatataa tttttaacaa 1260

ataaattcat tttatgttat acttttatac tatatactaa gtgttgctca aatgttttaa 1320

atgttagaat cttaaaatac gtgaacacat tatattatag gacggaggga taactgggag 1380

tagataattg atgaataatt gcattgagaa ttgataaaat atgagtattc tagtcttctt 1440

atttttttat tggtaccatg tgatagatga aaaataaact tattttggga cggagatagc 1500

tagtatttaa aattcaacat gtttatatat tttaagaagc aaattttttc agacgtttgg 1560

tgttaggaaa tca 1573

<210> 10

<211> 32

<212> DNA

＜213〉Oryza paddy rice (Orysa sativa L.)

<400> 10

gatggcgcgc catacctgac tgcgtgatac at 32

<210> 11

<211> 30

<212> DNA

＜213〉Oryza paddy rice (Orysa sativa L.)

<400> 11

attggcgcgc cgaagacccg ataaggctaa 30

<210> 12

<211> 31

<212> DNA

＜213〉Oryza paddy rice (Orysa sativa L.)

<400> 12

tatggcgcgc ccagaaacag caacgggagt g 31

<210> 13

<211> 32

<212> DNA

＜213〉Oryza paddy rice (Orysa sativa L.)

<400> 13

tatggc gcg cctgagacaa aggagtggca cg 32

<210> 14

<211> 30

<212> DNA

＜213〉Oryza paddy rice (Orysa sativa L.)

<400> 14

tcggcgcgcc ttgcgtgttc cccttgtaat 30

<210> 15

<211> 30

<212> DNA

＜213〉Oryza paddy rice (Orysa sativa L.)

<400> 15

acggcgcgcc tgcccaatct gctctgtctc 30

<210> 16

<211> 20

<212> DNA

<213> Binary vector pCAMBIA-1300

<400> 16

ctgctccata caagccaacc 20

<210> 17

<211> 19

<212> DNA

<213> Binary vector pCAMBIA-1300

<400> 17

tgtcctgcgg gtaaatagc 19

<210> 18

<211> 20

<212> DNA

<213> Binary vector pCAMBIA-1300

<400> 18

tttcagcgtg tcctctccaa 20

<210> 19

<211> 20

<212> DNA

<213> Binary vector pCAMBIA-1300

<400> 19

acatacgagc cggaagcata 20

Claims (4)

1. a rice blast resistant gene RMg7 or RMg8 or RMg9, its nucleotide sequence is respectively SEQ ID NO:1 or SEQ ID NO:4 or SEQ ID NO:7.

2. according to the described rice blast resistant gene RMg7 of claim 1 or RMg8 or the coded albumen of RMg9, its aminoacid sequence is respectively SEQ ID NO:2 or SEQ ID NO:5 or SEQ ID NO:8.

3. contain the carrier just like the rice blast resistant gene RMg7 described in the claim 1 or RMg8 or RMg9.

4. the coded application of albumen in preparation resisting rice blast bacteria medicine of RMg7 according to claim 2 or RMg8 or RMg9.

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