CN102727878A - Preparation method of porcine reproductive and respiratory syndrome inactivated vaccine (NVDC-JXA1 strain) by bioreactor and application thereof - Google Patents
Preparation method of porcine reproductive and respiratory syndrome inactivated vaccine (NVDC-JXA1 strain) by bioreactor and application thereof Download PDFInfo
- Publication number
- CN102727878A CN102727878A CN2012102311294A CN201210231129A CN102727878A CN 102727878 A CN102727878 A CN 102727878A CN 2012102311294 A CN2012102311294 A CN 2012102311294A CN 201210231129 A CN201210231129 A CN 201210231129A CN 102727878 A CN102727878 A CN 102727878A
- Authority
- CN
- China
- Prior art keywords
- microcarrier
- cell
- bioreactor
- nvdc
- respiratory syndrome
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
The invention provides a preparation method of porcine reproductive and respiratory syndrome inactivated vaccine (NVDC-JXA1 strain) by bioreactor and application thereof. The method comprises the following steps of: (1) culturing cells for preparing vaccine by a microcarrier, and selecting a bioreactor; (2) propagating and harvesting venom for preparing the vaccine; (3) preparing the vaccine; and (4) inspecting a finished product. The vaccine produced by the method has the advantages of low pollution probability, high virus titer (titer can be increased by 2 to 5 times), small difference among batches, stable product quality and small toxic reaction, so that the vaccine quality can be comprehensively improved.
Description
Technical field
The present invention relates to the veterinary biologics technical field; Particularly; Relate to the Porcine reproductive and respiratory syndrome technical field of vaccines, further, relate to method and application thereof that a kind of bioreactor prepares Porcine reproductive and respiratory syndrome inactivated vaccine (NVDC-JXA1 strain).
Background technology
Porcine reproductive and respiratory syndrome (PRRS) is claimed reproductive and respiratory syndrome again, is a kind of height contagious disease of the pig that caused by porcine reproductive and respiratory syndrome virus (PRRSV).The pig of all ages and classes, kind and sex all can infect, but with in-pig and 1 monthly age with the susceptible of interior piglet.This disease is a principal character with sow miscarriage, stillborn fetus, weak tire, mummy tire and piglet dyspnea, septicemia, high mortality etc.Highly pathogenic PRRS is a kind of acute, the high lethal infectious disease that is caused by the porcine reproductive and respiratory syndrome virus variant, and clinical manifestation is a main feature with fever, sickness rate height and mortality rate high " three-hypers ".Summer in 2006, this disease (claiming " unknown high fever of pigs " at that time) is popular in a big way in China, makes China's pig industry suffer the economic loss of unprecedented heaviness.Can PRRSV be divided into american type and Europe class according to dna homolog property and serology difference, popular in China mainly is the american type strain.Owing to still do not have this sick specific medicament of treatment at present, therefore use specificity vaccine to become the major measure of prevention and control PRRS.Developed the vaccine of two types of inactivated vaccine and attenuated live vaccines at present both at home and abroad, it is generally acknowledged the attenuated live vaccines good immune effect, and the inactivated vaccine safety has been better.Inactivated vaccine is the vaccine of early developing, and uses the main pig body generation humoral immune reaction that stimulates in back.Because of it is dead Seedling, so biggest advantage is safety, diffusing poison, is convenient to store and transportation.Bayer company has released inactivated vaccine PRRomiSetM first in 1997; The existing 20 tame enterprises of China are produced the PRRS inactivated vaccine by Ministry of Agriculture's approval at present: CH-1a strain (biotechnology development company of Harbin dimension section) and NVDC-JXA1 strain (19 families such as Guangdong Dahuanong Animal Health Products Co., Ltd.); Be mainly used in the immunity on the negative pig farm of PRRS, to control PRRS with reduce economic loss and play certain great.
Prepare in the process at the PRRS inactivated vaccine; Relate to the preparation technology of seedling with viral liquid; This is the key point of vaccine production, and realize that this mode of production has following shortcoming and this technology traditional preparation method is the mode of production through the rolling bottle cultured cell: 1) labor intensity is big; 2) differences between batches are big; 3) cell density is low, yields poorly; 4) pollute easily etc.Facts have proved that the amount that only relies on zooblast to produce virus through the rolling bottle training method is limited.In order to overcome the existing existing defective of PRRS inactivated vaccine production technology, seeks easy, efficiently Strain grown cultures condition to the raising vaccine quality, reducing production costs is significant.
In recent years, utilize bioreactor and microcarrier cultured cell, propagative viruses extensive in the bio-pharmaceuticals sector application.This technology has the following advantages: 1) the product differences between batches are little; 2) reduce the probability of germ contamination greatly; 3) constant product quality, immune side reaction is low; 4) be easy to amplify.If this technology is applied to the production of PRRS vaccine, has then not only kept the advantage of cell culture but also overcome the limited shortcoming of its production output.Though China's research is in this respect started late, Recent study is very active.The Chinese patent publication number is CN101612395A; Denomination of invention is that a kind of Chinese patent of cultivating the method for sensitive cells production blue-ear disease vaccine discloses a kind of method of in bioreactor, producing pig blue-ear disease vaccine with microcarrier cultivation sensitive cells; Used bioreactor is stirring type bioreactor, airlift bioreactor, Wave bioreactor and hollow fiber reactor, and training method adopts feeding culture or perfusion cultures; Public announcement of a patent application number is CN102038942A; Denomination of invention is that the Chinese patent of the method for applying biological reaction vessel suitability for industrialized production pig blue-ear disease vaccine proposes a kind of Cell Lift type bioreactor that utilizes; Adopt the tank switching formula operating technology of amplifying step by step between bioreactor, the High Density Cultivation of carrying out cell is to prepare the method for vaccine, and used bioreactor is a stirring type bioreactor; Said carrier is a Cytodex series microcarrier, adopts batch formula, stream to add or the perfusion cultures mode; Public announcement of a patent application number is CN102002482A; Denomination of invention is that pig breathes and the Chinese patent of the production method that the breeding difficulty syndrome virus is malicious then proposes a kind of production technology that torrent-filling type bioreactor is cultivated Marc-145 cells produce PRRS virus of using, and said carrier is a polyesteramide scraps of paper carriers; And public announcement of a patent application number is CN101831412 A; Denomination of invention is that a kind of Chinese patent and public announcement of a patent application of method of producing porcine reproductive and respiratory syndrome viruses (PRRSV) in large scale number is CN101979514 A; Denomination of invention is that the Chinese patent of PRRS virus and vaccine and the production method of the two all proposes a kind of method that adopts tidal type microcarrier suspension culture bioreactor to produce PRRS virus; Said microcarrier is lamellar, sphere or netted; Composition is polyester, gelatin or polysaccharide, preferably netted polyester fiber.Produce Porcine reproductive and respiratory syndrome inactivated vaccine (NVDC-JXA1 strain) and use stirring type bioreactor-spherical microcarrier system cultured cell, do not appear in the newspapers as yet.
Summary of the invention
For this reason, the invention provides method and application thereof that a kind of bioreactor prepares Porcine reproductive and respiratory syndrome inactivated vaccine (NVDC-JXA1 strain), the vaccine that this method is produced; Pollution probability is low; Virus titer high (its malicious valency can improve 2～5 times), differences between batches are little, constant product quality; Side reaction is little, can promote vaccine quality comprehensively.
Technical scheme of the present invention is following: a kind of bioreactor prepares the method and the application thereof of Porcine reproductive and respiratory syndrome inactivated vaccine (NVDC-JXA1 strain), comprises the steps:
(1) cultivates seedling with microcarrier and use cell: select bioreactor; Select spherical microcarrier to attach the carrier of growth as cell; Select the Marc-145 cell to use cell as seedling; With the Marc-145 cell through trypsinization liquid had digestive transfer culture; Use prescription to be adjusted into 7.2 cell growth medium continuation cultivation for DMEM culture medium, the pH that contains 7%～10% hyclone; Cultivation temperature is 37 ℃, when forming good monolayer, gets well-grown Marc-145 cell on the rolling bottle; Be prepared as cell suspension through the digestion of trypsinization liquid, the ratio in 5～20 cells of each microcarrier interpolation behind the cell counting is inoculated in the bioreactor that contains cell growth medium and microcarrier; Bioreactor is set following parameter: 37 ℃ of temperature, pH value 6.8～7.4, dissolved oxygen content 40%～60%, mixing speed 30～55rpm, CO2 ventilation 5～10%, carry out reaction vessel and control cultivation automatically;
(2) seedling is with the breeding and the results of venom: cultivate the back and waited to observe that cell covers with when forming fine and close monolayer basically on the microcarrier in 48～72 hours; Remove cell growth medium, the prescription that adds 1/4～1 working volume is that DMEM culture medium, the pH that contains 2%～5% hyclone is adjusted into 7.2 cell maintenance medium; Is that 0.0001～1.0 ratio is inoculated well-grown above-mentioned cell monolayer with porcine reproductive and respiratory syndrome virus NVDC-JXA1 strain kind poison according to infection multiplicity M.O.I.; Hatched 1～10 hour for 37 ℃; In bioreactor, supply the needed culture medium of bioreactor operate as normal then; And set following parameter: 37 ℃ of temperature, pH value 6.5～7.2, dissolved oxygen 40%～60%, mixing speed 30～45rpm, CO2 ventilation 5～10%, carry out reaction vessel and control cultivation automatically; Connect the poison back and whenever got microcarrier in the reaction vessel at a distance from 2 hours; With microscope observing cell pathological changes situation; Wait to observe that cell basically all comes off on the microcarrier, cytopathy reaches 80% when above; The stirring of stopped reaction device, treat that microcarrier all sinks to reactor bottom after, gather in the crops supernatant and microcarrier respectively; Multigelation 2 times through centrifugal or remove by filter cell debris, is preserved below-20 ℃ as semi-finished product; By " Chinese veterinary drug allusion quotation " appendix semi-finished product are carried out steriling test and viral level mensuration, viral level should be not less than 10
6.0TCID
50/ ml;
(3) vaccine preparation: the viral liquid that will be up to the standards is that 0.1% ratio adds formalin in final concentration, and mixing is put 37 ℃ of deactivations and taken out in 18 hours, puts 2～8 ℃ of preservations; Deactivation is got 96 parts of inactivation of viruses liquid after the assay was approved, adds 4 parts of the cooled tween 80s of autoclaving, fully stirs, and dissolves fully until tween 80, makes water.Get 94 parts of injection white oils, Si Ben-806 and part mix post-heating, add 2 parts of aluminium stearate again, with add be stirred to transparent till, autoclaving makes oil phase; Get 1.5 parts of oil phases and put stirring in the emulsifying filling, slowly add 1 part of water again, stirred 30～60 minutes with 3000r/min again after adding, make mixture fully emulsified, process Porcine reproductive and respiratory syndrome inactivated vaccine (NVDC-JXA1 strain);
(4) product inspection: examine " Porcine reproductive and respiratory syndrome inactivated vaccine (NVDC-JXA1 strain) is made and the tentative rules of check " requirement of passing through by the Ministry of Agriculture and test, each item index is all up to specification.
The said method for preparing Porcine reproductive and respiratory syndrome inactivated vaccine (NVDC-JXA1 strain) with bioreactor; In the step (1); Parameters such as bioreactor is can A.T.C, pH, dissolved oxygen and mixing speed; Be applicable to the bioreactor that microcarrier is cultivated, volume is 1.3L～1500L.
The said method for preparing Porcine reproductive and respiratory syndrome inactivated vaccine (NVDC-JXA1 strain) with bioreactor; In the step (1), microcarrier is spherical microcarrier, and addition is 2～10g/L; Need clean before use, sterilize, method is following: 1) soak microcarrier more than three hours with PBS; 2) clean microcarrier 3 times with PBS; 3) soak microcarrier with PBS, 121 ℃ of steam sterilizations 30 minutes.
Beneficial effect of the present invention is: according to the inventionly prepare the method for Porcine reproductive and respiratory syndrome inactivated vaccine (NVDC-JXA1 strain) with bioreactor, compare with traditional rolling bottle production technology, have the following advantages:
(1) use the vaccine that the present invention produces, pollution probability is low, virus titer high (its malicious valency can improve 2～5 times), and differences between batches are little, constant product quality, side reaction is little, can promote vaccine quality comprehensively;
(2) automaticity is high, and labor intensity is little, saves manpower, and output is big, occupation of land is little, with short production cycle, can effectively reduce production costs;
(3) the present invention adopts spherical microcarrier as cell culture vector, is easy to expand the scale of production fast, can overcome the characteristic that chip carrier is difficult to amplify.
The specific embodiment
Embodiment 1:
A kind of bioreactor prepares the method for Porcine reproductive and respiratory syndrome inactivated vaccine (NVDC-JXA1 strain), and step is following:
(1) cultivate seedling with microcarrier and use cell: select 7.5L NBS bioreactor, its working volume is 5L; Select the spherical microcarrier of CytodexI to attach the carrier of growth as cell, use amount is the 4g/L culture medium, the spherical microcarrier of weighing CytodexI, and it is cleaned, sterilizes, method is: 1) with an amount of PBS immersion 3 hours; 2) clean microcarrier 3 times with an amount of PBS; 3) add an amount of PBS and soak microcarrier, 121 ℃ of steam sterilizations 30 minutes; Select the Marc-145 cell to use cell as seedling; With the Marc-145 cell through trypsinization liquid had digestive transfer culture; Use prescription to be adjusted into 7.2 cell growth medium continuation cultivation for 90%DMEM culture medium, the pH that contains 10% hyclone; Cultivation temperature is 37 ℃, when forming good monolayer, gets well-grown Marc-145 cell on the rolling bottle; Be prepared as cell suspension through the digestion of trypsinization liquid, the ratio in 10 cells of each microcarrier interpolation behind the cell counting is inoculated in the bioreactor that contains cell growth medium and microcarrier; Bioreactor is set following parameter: 37 ℃ of temperature, pH value 7.0, dissolved oxygen content 50%, mixing speed 45rpm, CO2 ventilation 5%, carry out reaction vessel and control cultivation automatically;
(2) seedling is with the breeding and the results of venom: cultivate and waited to observe that cell covers with when forming fine and close monolayer basically on the microcarrier in back 48 hours; Remove cell growth medium, the prescription that adds 1/4～1 working volume is that 98%DMEM culture medium, the pH that contains 2% hyclone is adjusted into 7.2 cell maintenance medium; Is that 0.01 ratio is inoculated well-grown above-mentioned cell monolayer with porcine reproductive and respiratory syndrome virus NVDC-JXA1 strain kind poison according to infection multiplicity M.O.I.; Hatched 4 hours for 37 ℃; In bioreactor, supply the needed culture medium of bioreactor operate as normal then; And set following parameter: 37 ℃ of temperature, pH value 7.0, dissolved oxygen 50%, mixing speed 35rpm, CO2 ventilation 5%, carry out reaction vessel and control cultivation automatically; Connect the poison back and whenever got microcarrier in the reaction vessel at a distance from 2 hours; With microscope observing cell pathological changes situation; Wait to observe that cell basically all comes off on the microcarrier, cytopathy reaches 80% when above; The stirring of stopped reaction device, treat that microcarrier all sinks to reactor bottom after, gather in the crops supernatant and microcarrier respectively; Multigelation 2 times through centrifugal or remove by filter cell debris, is preserved below-20 ℃ as semi-finished product; By " Chinese veterinary drug allusion quotation " appendix semi-finished product are carried out steriling test and viral level mensuration; The result gathers in the crops viral liquid 10L altogether, and steriling test is qualified, and viral level reaches 10
7.5TCID
50/ ml.
(3) vaccine preparation: the viral liquid that will be up to the standards is that 0.1% ratio adds formalin in final concentration, and mixing is put 37 ℃ of deactivations and taken out in 18 hours, puts 2～8 ℃ of preservations; Deactivation is got 96 parts of inactivation of viruses liquid after the assay was approved, adds 4 parts of the cooled tween 80s of autoclaving, fully stirs, and dissolves fully until tween 80, makes water.Get 94 parts of injection white oils, Si Ben-806 and part mix post-heating, add 2 parts of aluminium stearate again, with add be stirred to transparent till, autoclaving makes oil phase; Get 1.5 parts of oil phases and put stirring in the emulsifying filling, slowly add 1 part of water again, stirred 30 minutes with 3000r/min again after adding, make mixture fully emulsified, process Porcine reproductive and respiratory syndrome inactivated vaccine (NVDC-JXA1 strain);
(4) product inspection: examine " Porcine reproductive and respiratory syndrome inactivated vaccine (NVDC-JXA1 strain) is made and the tentative rules of check " requirement of passing through by the Ministry of Agriculture and test, each item index is all up to specification, sees table 1 for details.
Table 1 prepares Porcine reproductive and respiratory syndrome inactivated vaccine (NVDC-JXA1 strain) assay with bioreactor
Above content is to combine concrete preferred implementation to the further explain that the present invention did, and can not assert that practical implementation of the present invention is confined to these explanations.For the those of ordinary skill of technical field under the present invention, under the prerequisite that does not break away from the present invention's design, its framework form can be flexible and changeable, can the subseries product.Just make some simple deduction or replace, all should be regarded as belonging to the scope of patent protection that the present invention is confirmed by claims of being submitted to.
Claims (3)
1. a bioreactor prepares the method and the application thereof of Porcine reproductive and respiratory syndrome inactivated vaccine (NVDC-JXA1 strain), it is characterized in that, comprises the steps:
(1) cultivates seedling with microcarrier and use cell: select bioreactor; Select spherical microcarrier to attach the carrier of growth as cell; Select the Marc-145 cell to use cell as seedling; With the Marc-145 cell through trypsinization liquid had digestive transfer culture; Use prescription to be adjusted into 7.2 cell growth medium continuation cultivation for DMEM culture medium, the pH that contains 7%～10% hyclone; Cultivation temperature is 37 ℃, when forming good monolayer, gets well-grown Marc-145 cell on the rolling bottle; Be prepared as cell suspension through the digestion of trypsinization liquid, the ratio in 5～20 cells of each microcarrier interpolation behind the cell counting is inoculated in the bioreactor that contains cell growth medium and microcarrier; Bioreactor is set following parameter: 37 ℃ of temperature, pH value 6.8～7.4, dissolved oxygen content 40%～60%, mixing speed 30～55rpm, CO2 ventilation 5～10%, carry out reaction vessel and control cultivation automatically;
(2) seedling is with the breeding and the results of venom: cultivate the back and waited to observe that cell covers with when forming fine and close monolayer basically on the microcarrier in 48～72 hours; Remove cell growth medium, the prescription that adds 1/4～1 working volume is that DMEM culture medium, the pH that contains 2%～5% hyclone is adjusted into 7.2 cell maintenance medium; Is that 0.0001～1.0 ratio is inoculated well-grown above-mentioned cell monolayer with porcine reproductive and respiratory syndrome virus NVDC-JXA1 strain kind poison according to infection multiplicity M.O.I.; Hatched 1～10 hour for 37 ℃; In bioreactor, supply the needed culture medium of bioreactor operate as normal then; And set following parameter: 37 ℃ of temperature, pH value 6.5～7.2, dissolved oxygen 40%～60%, mixing speed 30～45rpm, CO2 ventilation 5～10%, carry out reaction vessel and control cultivation automatically; Connect the poison back and whenever got microcarrier in the reaction vessel at a distance from 2 hours; With microscope observing cell pathological changes situation; Wait to observe that cell basically all comes off on the microcarrier, cytopathy reaches 80% when above; The stirring of stopped reaction device, treat that microcarrier all sinks to reactor bottom after, gather in the crops supernatant and microcarrier respectively; Multigelation 2 times through centrifugal or remove by filter cell debris, is preserved below-20 ℃ as semi-finished product; By " Chinese veterinary drug allusion quotation " appendix semi-finished product are carried out steriling test and viral level mensuration, viral level should be not less than 10
6.0TCID
50/ ml;
(3) vaccine preparation: the viral liquid that will be up to the standards is that 0.1% ratio adds formalin in final concentration, and mixing is put 37 ℃ of deactivations and taken out in 18 hours, puts 2～8 ℃ of preservations; Deactivation is got 96 parts of inactivation of viruses liquid after the assay was approved, adds 4 parts of the cooled tween 80s of autoclaving, fully stirs, and dissolves fully until tween 80, makes water; Get 94 parts of injection white oils, Si Ben-80 6 and part mix post-heating, add 2 parts of aluminium stearate again, with add be stirred to transparent till, autoclaving makes oil phase; Get 1.5 parts of oil phases and put stirring in the emulsifying filling, slowly add 1 part of water again, stirred 30～60 minutes with 3000r/min again after adding, make mixture fully emulsified, process Porcine reproductive and respiratory syndrome inactivated vaccine (NVDC-JXA1 strain);
(4) product inspection: examine " Porcine reproductive and respiratory syndrome inactivated vaccine (NVDC-JXA1 strain) is made and the tentative rules of check " requirement of passing through by the Ministry of Agriculture and test, each item index is all up to specification.
2. method and the application thereof for preparing Porcine reproductive and respiratory syndrome inactivated vaccine (NVDC-JXA1 strain) with bioreactor as claimed in claim 1; It is characterized in that; In the step (1); Parameters such as bioreactor is can A.T.C, pH, dissolved oxygen and mixing speed are applicable to the bioreactor that microcarrier is cultivated, and volume is 1.3L～1500L.
3. method and the application thereof for preparing Porcine reproductive and respiratory syndrome inactivated vaccine (NVDC-JXA1 strain) with bioreactor as claimed in claim 1; It is characterized in that; In the step (1), microcarrier is spherical microcarrier, and addition is 2～10g/L; Need clean before use, sterilize, method is following: 1) soak microcarrier more than three hours with PBS; 2) clean microcarrier 3 times with PBS; 3) soak microcarrier with PBS, 121 ℃ of steam sterilizations 30 minutes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012102311294A CN102727878A (en) | 2012-07-04 | 2012-07-04 | Preparation method of porcine reproductive and respiratory syndrome inactivated vaccine (NVDC-JXA1 strain) by bioreactor and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012102311294A CN102727878A (en) | 2012-07-04 | 2012-07-04 | Preparation method of porcine reproductive and respiratory syndrome inactivated vaccine (NVDC-JXA1 strain) by bioreactor and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102727878A true CN102727878A (en) | 2012-10-17 |
Family
ID=46984640
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012102311294A Pending CN102727878A (en) | 2012-07-04 | 2012-07-04 | Preparation method of porcine reproductive and respiratory syndrome inactivated vaccine (NVDC-JXA1 strain) by bioreactor and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102727878A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105106948A (en) * | 2015-06-29 | 2015-12-02 | 北京市兽医生物药品厂 | Method for preparing porcine reproductive and respiratory syndrome inactivated vaccine by using cell factory |
| CN106237323A (en) * | 2016-09-22 | 2016-12-21 | 齐鲁动物保健品有限公司 | Pig blue-ear disease purified vaccine and preparation method thereof |
| CN108374058A (en) * | 2018-02-13 | 2018-08-07 | 中国动物疫病预防控制中心（农业部屠宰技术中心） | A kind of highly pathogenic PRRSV nucleic acid standard substance and preparation method thereof |
| CN111454882A (en) * | 2020-04-15 | 2020-07-28 | 杭州佑本动物疫苗有限公司 | Process for producing blue ear virus by culturing Marc-145 cells by microcarrier |
| CN114438041A (en) * | 2022-01-28 | 2022-05-06 | 南阳师范学院 | Bovine enterovirus type 1 isolated strain and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102038942A (en) * | 2010-09-15 | 2011-05-04 | 武汉中博生物股份有限公司 | Method for industrially producing porcine reproductive and respiratory syndrome (PRRS) vaccines by utilizing bioreactor |
-
2012
- 2012-07-04 CN CN2012102311294A patent/CN102727878A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102038942A (en) * | 2010-09-15 | 2011-05-04 | 武汉中博生物股份有限公司 | Method for industrially producing porcine reproductive and respiratory syndrome (PRRS) vaccines by utilizing bioreactor |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105106948A (en) * | 2015-06-29 | 2015-12-02 | 北京市兽医生物药品厂 | Method for preparing porcine reproductive and respiratory syndrome inactivated vaccine by using cell factory |
| CN106237323A (en) * | 2016-09-22 | 2016-12-21 | 齐鲁动物保健品有限公司 | Pig blue-ear disease purified vaccine and preparation method thereof |
| CN108374058A (en) * | 2018-02-13 | 2018-08-07 | 中国动物疫病预防控制中心（农业部屠宰技术中心） | A kind of highly pathogenic PRRSV nucleic acid standard substance and preparation method thereof |
| CN111454882A (en) * | 2020-04-15 | 2020-07-28 | 杭州佑本动物疫苗有限公司 | Process for producing blue ear virus by culturing Marc-145 cells by microcarrier |
| CN114438041A (en) * | 2022-01-28 | 2022-05-06 | 南阳师范学院 | Bovine enterovirus type 1 isolated strain and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101934074B (en) | Porcine circovirus II vaccine and production method thereof | |
| CN101804203A (en) | Method for mass production of pseudorabies virus vaccine | |
| CN102091329B (en) | Preparation method of inactivated porcine parvovirus vaccine and product thereof | |
| CN101947318B (en) | Method for preparing porcine parvovirus inactivated vaccines | |
| CN101665781B (en) | High-titer Porcine circovirus 2-type cultured cell, preparation method and use thereof | |
| CN103550771B (en) | The production method of transmissible gastro-enteritis virus vaccine | |
| CN101955915B (en) | Method for producing influenza virus vaccine | |
| CN102690791A (en) | Method for generating porcine pseudorabies virus by culturing ST cell in microcarrier of bioreactor | |
| CN101818131A (en) | Production method for influenza virus vaccines | |
| CN102727878A (en) | Preparation method of porcine reproductive and respiratory syndrome inactivated vaccine (NVDC-JXA1 strain) by bioreactor and application thereof | |
| CN101926991A (en) | Classical swine fever virus vaccine and production method thereof | |
| CN102260649A (en) | Infectious bursal disease virus (IBDV) and method for preparing inactivated vaccines and combined vaccines by breeding IBDV with chick-embryo cell system and bioreactor | |
| CN101869702A (en) | Vaccine produced by suspended microcarrier cell culture system and method for producing vaccine | |
| CN102133398A (en) | Method for industrially producing animal influenza vaccine by using bioreactor | |
| CN103394081B (en) | WAVE wave bioreactor is utilized to produce the method for avian influenza vaccine | |
| CN102727877A (en) | Method for preparing highly pathogenic porcine reproductive and respiratory syndrome live vaccine (JXA1-R strain) by utilizing bioreactor and application thereof | |
| CN101831412A (en) | Method for producing porcine reproductive and respiratory syndrome viruses (PRRSV) in large scale | |
| CN102660510A (en) | Production method of porcine transmissible gastroenteritis virus by utilizing bioreactor | |
| CN103861097A (en) | Method for preparing porcine epizootic diarrhea inactivated vaccines and product thereof | |
| CN102038946A (en) | Method for industrially producing pseudorabies vaccine by using bioreactor | |
| CN102002481B (en) | Production method of porcine reproductive and respiratory syndrome virus | |
| CN108421037A (en) | A kind of porcine pseudorabies/porcine parvovirus bivalent inactivated vaccine and its culture preparation method that suspends | |
| CN105385661A (en) | Porcine circovirus type 2 large-scale cultivation method and applications thereof | |
| CN102038945A (en) | Method for industrially producing swine parvovirus vaccine by utilizing bioreactor | |
| CN103320393B (en) | Method for culturing gosling plague virus by use of goose embryo continuous cell line and bioreactor |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20121017 |