CN102716684A - Method for eutrophic water pollution treatment through polylactic acid fiber membrane immobilized compound enzyme technology - Google Patents
Method for eutrophic water pollution treatment through polylactic acid fiber membrane immobilized compound enzyme technology Download PDFInfo
- Publication number
- CN102716684A CN102716684A CN2012102330435A CN201210233043A CN102716684A CN 102716684 A CN102716684 A CN 102716684A CN 2012102330435 A CN2012102330435 A CN 2012102330435A CN 201210233043 A CN201210233043 A CN 201210233043A CN 102716684 A CN102716684 A CN 102716684A
- Authority
- CN
- China
- Prior art keywords
- eutrophic
- fiber membrane
- spinning
- enzyme
- water body
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims abstract description 22
- 239000000835 fiber Substances 0.000 title claims abstract description 17
- 239000012528 membrane Substances 0.000 title claims abstract description 13
- 102000004190 Enzymes Human genes 0.000 title claims description 26
- 108090000790 Enzymes Proteins 0.000 title claims description 26
- 239000004626 polylactic acid Substances 0.000 title abstract description 16
- 229920000747 poly(lactic acid) Polymers 0.000 title abstract description 15
- 238000005516 engineering process Methods 0.000 title abstract description 8
- 238000003911 water pollution Methods 0.000 title description 3
- 150000001875 compounds Chemical class 0.000 title 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 50
- 229940088598 enzyme Drugs 0.000 claims abstract description 33
- 230000002538 fungal effect Effects 0.000 claims abstract description 14
- 108010029541 Laccase Proteins 0.000 claims abstract description 8
- 239000004365 Protease Substances 0.000 claims abstract description 8
- 108090000526 Papain Proteins 0.000 claims abstract description 7
- 102000000019 Sterol Esterase Human genes 0.000 claims abstract description 7
- 108010055297 Sterol Esterase Proteins 0.000 claims abstract description 7
- 229940055729 papain Drugs 0.000 claims abstract description 7
- 235000019834 papain Nutrition 0.000 claims abstract description 7
- 238000009987 spinning Methods 0.000 claims description 18
- 230000008439 repair process Effects 0.000 claims description 8
- 108010059892 Cellulase Proteins 0.000 claims description 7
- 229920001400 block copolymer Polymers 0.000 claims description 7
- 229940106157 cellulase Drugs 0.000 claims description 7
- 238000003756 stirring Methods 0.000 claims description 6
- 101150039403 ams gene Proteins 0.000 claims description 5
- 238000005303 weighing Methods 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 4
- 238000002347 injection Methods 0.000 claims description 3
- 239000007924 injection Substances 0.000 claims description 3
- 239000002245 particle Substances 0.000 claims description 3
- 239000008055 phosphate buffer solution Substances 0.000 claims description 3
- 239000004094 surface-active agent Substances 0.000 claims description 3
- 230000010148 water-pollination Effects 0.000 claims description 3
- 229920003171 Poly (ethylene oxide) Polymers 0.000 claims description 2
- 238000013019 agitation Methods 0.000 claims description 2
- -1 polyoxyethylene Polymers 0.000 claims description 2
- 238000004088 simulation Methods 0.000 claims description 2
- 230000005611 electricity Effects 0.000 claims 2
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 230000005855 radiation Effects 0.000 claims 1
- 238000003786 synthesis reaction Methods 0.000 claims 1
- 230000008901 benefit Effects 0.000 abstract description 5
- 238000001523 electrospinning Methods 0.000 abstract description 4
- 239000003344 environmental pollutant Substances 0.000 abstract description 3
- 231100000719 pollutant Toxicity 0.000 abstract description 3
- 235000010469 Glycine max Nutrition 0.000 abstract 1
- 244000068988 Glycine max Species 0.000 abstract 1
- 108010046334 Urease Proteins 0.000 abstract 1
- 102000004139 alpha-Amylases Human genes 0.000 abstract 1
- 108090000637 alpha-Amylases Proteins 0.000 abstract 1
- 229940024171 alpha-amylase Drugs 0.000 abstract 1
- 238000009360 aquaculture Methods 0.000 abstract 1
- 229920002678 cellulose Polymers 0.000 abstract 1
- 239000001913 cellulose Substances 0.000 abstract 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 abstract 1
- 238000005067 remediation Methods 0.000 abstract 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 16
- 238000012851 eutrophication Methods 0.000 description 13
- 229910052757 nitrogen Inorganic materials 0.000 description 8
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 7
- 229910052698 phosphorus Inorganic materials 0.000 description 7
- 239000011574 phosphorus Substances 0.000 description 7
- 239000000463 material Substances 0.000 description 6
- 238000010041 electrostatic spinning Methods 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 230000007613 environmental effect Effects 0.000 description 4
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 239000003054 catalyst Substances 0.000 description 3
- 230000003197 catalytic effect Effects 0.000 description 3
- 230000002950 deficient Effects 0.000 description 3
- 230000002255 enzymatic effect Effects 0.000 description 3
- 239000000499 gel Substances 0.000 description 3
- 230000006872 improvement Effects 0.000 description 3
- 238000011065 in-situ storage Methods 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 229930002875 chlorophyll Natural products 0.000 description 2
- 235000019804 chlorophyll Nutrition 0.000 description 2
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000002121 nanofiber Substances 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 239000000741 silica gel Substances 0.000 description 2
- 229910002027 silica gel Inorganic materials 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
- 108090001060 Lipase Proteins 0.000 description 1
- 239000004367 Lipase Substances 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 108010091086 Recombinases Proteins 0.000 description 1
- 102000018120 Recombinases Human genes 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 229920002988 biodegradable polymer Polymers 0.000 description 1
- 239000004621 biodegradable polymer Substances 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- 230000005686 electrostatic field Effects 0.000 description 1
- 230000009931 harmful effect Effects 0.000 description 1
- 230000005661 hydrophobic surface Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 229910010272 inorganic material Inorganic materials 0.000 description 1
- 239000011147 inorganic material Substances 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- VUZPPFZMUPKLLV-UHFFFAOYSA-N methane;hydrate Chemical compound C.O VUZPPFZMUPKLLV-UHFFFAOYSA-N 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000001000 micrograph Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 235000019419 proteases Nutrition 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000000935 solvent evaporation Methods 0.000 description 1
- 238000002336 sorption--desorption measurement Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000002352 surface water Substances 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Images
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/30—Wastewater or sewage treatment systems using renewable energies
- Y02W10/37—Wastewater or sewage treatment systems using renewable energies using solar energy
Landscapes
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
The invention relates to a multi-enzyme immobilized electrospun fiber membrane used for treating a eutrophic polluted water body. By using an electrospinning technology, a multi-enzyme system which comprises alpha-amylase, hormone sensitive lipase, papain, fungal cellulose, fungal laccase, soybean urease and the like and can decompose various organic matters causing eutrophic water body pollution is immobilized in a polylactic acid electrospun fiber membrane and directly covers the surface of the eutrophic polluted water body to catalytically degrade various nitrogen-containing organic matters, so as to achieve the goal of reducing the eutrophic pollution in the water body. The polylactic acid electrospun fiber membrane which carries the multi-enzyme system can rapidly and conveniently remove typical eutrophic pollutants in the water body in time to rapidly recover water quality. Compared with other eutrophic pollution treatment techniques, the multi-enzyme immobilized electrospun fiber membrane has the advantages of high efficiency and good performance, can be expected to be used for remediation of urban moderate or severe eutrophic polluted water bodies or in the aquiculture field.
Description
Technical field
The invention belongs to technical field of water pollution control, be specially a kind of active tunica fibrosa preparation of immobilization complex enzyme and method for using that is used to handle eutrophicated polluted water.
Background technology
Water environment protection is a fundamental state policy of China.The urban rivers and lakes water system is the important component part of urban environment system, but in recent years, wawter bloom in various degree takes place the urban water system of China year after year, and a large amount of algaes that grow in the urban rivers and lakes have brought harmful effect for urban ecological environment and reputation.Wawter bloom prevention and improvement are the important measures of protection urban and rural environment, and it is an important agenda of building a harmonious socialist society that national Eleventh Five-Year Plan program outline has also clearly proposed the control of comprehensive environmental improvement and eutrophicated polluted water.In the river Lake Water Body was polluted, eutrophication was one of the most general, that harm is maximum water environmental problems.According to statistics, present lake, the reservoir more than 60% of China is in the eutrophication level, wherein heavy eutrophy and ultraly eutrophicly account for 20%, and therefore, eutrophication has become the great water environmental problems that China's river lake water protection of resources faces.The reason that causes body eutrophication is to a great extent owing to agricultural area source pollutants (like auxotype pollutants such as nitrogen, phosphorus).Show that according to the Beijing city ecological Studies Beijing city water system comprises that in lake, city and the river, the nitrogen and phosphorus pollution degree is constantly aggravated in recent years.Research and analyse according to relevant, cause the nitrogen of eutrophication in lake and the surface water, the input that phosphorus mainly comes from terrestrial ecosystems.Nitrogen, phosphorus load have many non-point pollutions from agricultural more than 50% in most of lakes, and part is from the input of urban life system.
Because the seriousness that body eutrophication pollutes, its improvement method has received a large amount of research organizations pay much.Though developed various water bodies eutrophication control method; But be subject to problems such as cost height, secondary pollution be serious; Particularly chemicals possibly cause irreversible heavy damage to water environment self-recovery function, makes really to be used for method that on-the-spot original position repairs seldom.Therefore the method for seeking economical, efficient, eco-friendly control body eutrophication pollution remains one of popular topic of Related Research Domain.In the various processing modes, it is safe and effective that biological restoration is considered to, and meets the green repair mode of sustainable development.In recent years, utilize biological enzyme technology to handle waste water both at home and abroad and obtained certain progress.Aspect the water body reparation, but the MicroPan Aqua of company of enzyme nation development contains biology enzyme and the multiple microorganisms that can be used for water treatment such as lipase, protease, cellulase, eutrophication water is administered have better effects.But this kind enzyme preparation is to environment sensitive, easy inactivation, and can't reclaim, and directly limited the economy of its actual use.
The research of immobilised enzymes and application are to realize that enzyme is reused and the effective means of improved stability.In recent years the immobilization of enzyme has been carried out than extensive studies, the fixation support of employing comprises multiple materials such as silica gel, active carbon, bead, shitosan.Enzyme both can reach the living things catalysis effect after immobilization, can realize the recycling of enzyme again, reduced the cost of enzyme purification, made things convenient for separating of enzyme and reactant.But also there is defective in actual use in various degree in existing enzyme immobilization material.As with inorganic material such as silica gel, active carbons as fixation support, exist separate with water body, the surface with defectives such as immobilized enzyme bonding is not tight; Then there are defectives such as processed complex, character instability in shitosans etc.Existing fixed formed material preparation technology is loaded down with trivial details in addition, method for using is complicated, must be by certain backing material, and can't in-situ applications.
The high-voltage electrostatic spinning acid fiber by polylactic can be used for enzyme immobilization carrier.This technology is that a kind of applying high voltage electric field flows charged Polymer Solution or distortion in electrostatic field, then because solvent evaporation or melt cool off solidifies, finally produces the process of the fiber of diameter tens nanometer to tens micron.Electrospun fiber membrane has that specific area is big, porosity is high and advantage such as absorption property is strong, is a kind of potential enzyme immobilization matrix.With its carrier as immobilised enzymes, help enzyme-to-substrate and fully contact, can effectively improve the catalytic efficiency of enzyme, and Separation and Recovery from reaction system easily.
PLA (PLA) is to study one of most active Biodegradable polymer material in recent years in the world; Can be degraded to carbon dioxide and water gradually fully at occurring in nature; As the immobilization laccase carrier, have excellent biological compatibility, can't cause secondary pollution to environment.Prepare polylactic acid nano fiber with electrostatic spinning technique and can obtain the large-area active function material that can be used for the enzyme load quickly and efficiently.Carry the enzyme nano fibrous membrane with what this technology prepared gained, have that specific area is big, enzymatic activity is high, catalytic performance is stable, a long service life, the less advantage such as environmental factor interference that receives, be expected to be used for the reparation of extensive body eutrophication Pollution in Situ.
Summary of the invention
The objective of the invention is to propose a kind of preparation method and method for using that body eutrophication pollutes that be used to handle with high catalytic activity, bigger serface, callable multienzyme loaded with nano tunica fibrosa.This tunica fibrosa is taken into account the recuperability of enzymatic high efficiency and nanofiber, so have better practical application prospect.
The present invention is through adopting following technical scheme to realize the foregoing invention purpose:
Multienzyme immobilization nano fibrous membrane provided by the invention is to be carrier with the poly-lactic acid in high molecular weight with hydrophobic surface, and the multiple mixed enzyme that will comprise AMS, hormone-sensitive lipase, papain, fungal cellulase, fungal laccase, arlco-urease is through absorption and crosslinked being fixed on this carrier.The implementation method that this tunica fibrosa is used for the body eutrophication reparation comprises that specifically the preparation of spinning solution and water body repair two parts.For realizing the in-situ treatment of polluted-water, multienzyme immobilization process and water body repair process carry out simultaneously, and concrete steps comprise:
1) a certain amount of PLA particle is dissolved in the carrene, adds hydrophily block interpolymers surfactant, evenly mix the back stirring and obtain PLA/block copolymer mixed gel.
2) taking by weighing a certain amount of and AMS, hormone-sensitive lipase, papain, fungal cellulase, fungal laccase, arlco-urease ratio, to be dissolved in pH value be 6.9 phosphate buffer solution, and vigorous stirring is to dissolving fully;
3) with step 1) and step 2) in the dichloromethane solution and the aqueous solution of gained, vigorous stirring is fully mixed the back and is obtained spinning presoma suspension, places in the refrigerator subsequent use;
4) high-voltage electrostatic spinning apparatus is made up of high voltage source, spinning head, and positive source is connected with spinning head, and negative pole is connected with lead with water body to be repaired; With 3) in gained spinning presoma suspension be incorporated in the high-voltage electrostatic spinning device; Reconcile supply voltage spinning head and water surface spacing, obtain the injection of steady and continuous.
5) spray certain hour after, the water body to be repaired of electric adrift spinning enzyme membrane on the water surface placed under the sunlight tans by the sun, the timing sampling analysis is to assess the repair ability of this system to eutrophicated polluted water.
High molecular polymer PLA described in the present invention is that racemic, viscosity average molecular weigh are about 100,000; Polyoxyethylene-poly-oxypropylene polyoxyethylene block copolymer P123 described in the step 1), its molecular formula is PEO20-PPO70-PEO20, and molecular weight is 5800 gram/moles, and the consumption of P123 is 5% of a PLA quality.In the method for the present invention, AMS, hormone-sensitive lipase, papain, fungal cellulase, fungal laccase, arlco-urease are provided by Shanghai pattern ocean biotech company, are purified grade.
The invention has the advantages that: the present invention utilizes electrostatic spinning technique that multiple enzyme catalyst with eutrophicated polluted water repair ability directly is fixed in the electrospun fiber membrane altogether, is prone to loss, poor stability, problem such as can not recycles thereby solved resolvase.And, the electro spinning nanometer fiber membrane of this load multi-enzyme system is applied to the eutrophicated polluted water aspect has advantage efficient, convenient, stable, non-secondary pollution.
Description of drawings
Fig. 1 is the scanning electron microscope image of polylactic acid electrospinning fibre immobilized multienzyme catalyst;
Fig. 2 is using polylactic acid electrospinning fibre immobilized multienzyme catalyst of the present invention to repair in the process of eutrophicated polluted water, and its each item water quality index is with handling change of time.
The practical implementation method
Embodiment 1
Take by weighing 25 gram PLA particles and be dissolved in 20 milliliters of carrene, add 0.5 gram hydrophily block interpolymers surfactant P123, evenly the mixing back is stirred and is obtained PLA/block copolymer mixed gel.Taking by weighing mass ratio is 1.0: 15: 7.0: 4.5: 0.25: 8.8 AMS, hormone-sensitive lipase, papain, fungal cellulase, fungal laccase, arlco-urease; Amounting to 500 milligrams, to be dissolved in 2 milliliters of pH values be 6.9 phosphate buffer solution; Vigorous stirring is to dissolving fully; Mix mutually with PLA/block copolymer mixed gel, high degree of agitation is introduced device for spinning and is waited to spin.
Target is repaired water body appearance and is picked up from artificial lake in the park, North 3rd Ring Road, Beijing, and sampled point is positioned at 3 meters of loke shores, 0.5 meter of the depth of water, and 5 liters of sampling volumes, its water quality index is: total nitrogen 3.8 mg/litre; Total phosphorus 0.20 mg/litre; The COD42.69 mg/litre; Chlorophyll 66.57 micrograms per litre; Dissolved oxygen 5.28 mg/litre; 23 centimetres of transparencies.Above-mentioned water sample is placed the pond, laboratory (30 centimetres of 30 cm x, 30 cm x) of simulation lake environment.
Electric spinning equipment is placed the top, pond, and reconciling high-voltage power voltage is 15 kilovolts, 10 centimetres of spinning head and water surface spacings, the injection stream of acquisition steady and continuous.Treat after 10 minutes that tunica fibrosa reaches certain thickness, stops spinning.This sample spinning product (full-automatic hole of SSA-4000 series and specific surface analyzer, Beijing Bi Aode Electron Technology Co., Ltd) on nitrogen adsorption desorption specific area pore size distribution analyzer carries out specific area measuring, and its specific area is 46.2 meters squared per gram.Under (JSM-6700F awkward silence at a meeting emission scan electron microscope, company of NEC) SEM, carry out the fiber pattern and characterize, its average fibre diameter 50～220 nanometers, distribution of fiber diameters is even, smooth surface.
After adding water quality reparation fixed enzyme membrane, will simulate the pond and place under strong sunshine, with the illumination decomposition of realization eutrophication pollution thing and the volatilization of low-boiling-point organic compound, and improve water temperature promotion enzymatic effect.After handling certain hour, water quality takes a turn for the better gradually.After 5 hours, the concentration of total nitrogen, total phosphorus, COD and dissolved oxygen is changed to 2.98,0.15,40.15,6.17 mg/litre respectively in the water quality; After 10 hours, above-mentioned four indices is changed to 2.54,0.02,36.26,6.66 mg/litre; After 24 hours, final water quality index is: total nitrogen 1.95 mg/litre; Total phosphorus 0.04 mg/litre; COD 15.19 mg/litre; Chlorophyll 8.89 micrograms per litre; Dissolved oxygen 8.12 mg/litre; 53 centimetres of transparencies.
Claims (4)
1. method of repairing eutrophicated polluted water with multi-enzyme system load electricity spinning nano fibrous membrane, it is characterized in that: the electricity spinning fibre film and the use thereof of load multi-enzyme system are: take by weighing 25 gram PLA particles and be dissolved in 20 milliliters of carrene, add 0.5 gram hydrophily block interpolymers surfactant P123; Stir and mix back acquisition PLA/block copolymer mixed gel; Take by weighing gross mass and be 500 milligrams AMS, hormone-sensitive lipase, papain, fungal cellulase, fungal laccase, arlco-urease, be dissolved in 2 milliliters of pH values and be 6.9 phosphate buffer solution, vigorous stirring is to dissolving fully; Mix mutually with PLA/block copolymer mixed gel; High degree of agitation is introduced device for spinning and is waited to spin, and takes pollution water sample to place the pond, laboratory (30 centimetres of 30 cm x, 30 cm x) of simulation lake environment, and electric spinning equipment is placed the top, pond; Reconciling high-voltage power voltage is 15 kilovolts; 10 centimetres of spinning head and water surface spacings, the injection stream of acquisition steady and continuous treats after 10 minutes that tunica fibrosa reaches certain thickness; Stop spinning, the eutrophicated polluted water repair is carried out in beginning under solar radiation.
2. the method described in claim 1, it is characterized in that: wherein the polyoxyethylene-poly-oxypropylene polyoxyethylene block copolymer described in the synthesis step of carrier is P123, and its molecular formula is PEO20-PPO70-PEO20, and molecular weight is 5800 gram/moles.
3. the method described in claim 1, it is characterized in that: the consumption of P123 is 2% of a PLA quality.
4. the method described in claim 1; It is characterized in that: in the used multi-enzyme system of immobilization, the mass ratio of various enzymes is an AMS: hormone-sensitive lipase: papain: fungal cellulase: fungal laccase: arlco-urease=1.0: 15: 7.0: 4.5: 0.25: 8.8.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210233043.5A CN102716684B (en) | 2012-07-06 | 2012-07-06 | Method for eutrophic water pollution treatment through polylactic acid fiber membrane immobilized compound enzyme technology |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210233043.5A CN102716684B (en) | 2012-07-06 | 2012-07-06 | Method for eutrophic water pollution treatment through polylactic acid fiber membrane immobilized compound enzyme technology |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102716684A true CN102716684A (en) | 2012-10-10 |
| CN102716684B CN102716684B (en) | 2014-04-16 |
Family
ID=46942629
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210233043.5A Active CN102716684B (en) | 2012-07-06 | 2012-07-06 | Method for eutrophic water pollution treatment through polylactic acid fiber membrane immobilized compound enzyme technology |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102716684B (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104032400A (en) * | 2014-06-19 | 2014-09-10 | 宜春学院 | Biological enzyme/lignocellulose composite fiber and preparation method thereof |
| CN108251409A (en) * | 2018-01-11 | 2018-07-06 | 山东交通学院 | A kind of complex microorganism guided missile material of absorption degradation greasy dirt and preparation method thereof |
| CN108483678A (en) * | 2018-05-31 | 2018-09-04 | 深圳市碧园环保技术有限公司 | A kind of biological zymophore and laying method for riverbed Water Ecological Recovery |
| CN108743929A (en) * | 2018-06-14 | 2018-11-06 | 四川大学 | A kind of preparation method and purposes of the urase gel micro-ball as urea scavenger |
| CN109012219A (en) * | 2018-09-03 | 2018-12-18 | 安徽工程大学 | A kind of preparation method of fixation in situ laccase ultrafiltration membrane, a kind of processing method of phenol wastewater |
| RU2677873C2 (en) * | 2017-07-03 | 2019-01-22 | федеральное государственное бюджетное образовательное учреждение высшего образования "Воронежский государственный университет" (ФГБОУ ВО "ВГУ") | Method of obtaining a heterogeneous drug based on papain |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1928081A (en) * | 2006-09-28 | 2007-03-14 | 武汉益生泉生物科技开发有限责任公司 | Artificial biological film and preparation method |
| CN101891292A (en) * | 2010-07-27 | 2010-11-24 | 北京师范大学 | Method for removing trace polycyclic aromatic hydrocarbon from water through quick adsorption |
-
2012
- 2012-07-06 CN CN201210233043.5A patent/CN102716684B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1928081A (en) * | 2006-09-28 | 2007-03-14 | 武汉益生泉生物科技开发有限责任公司 | Artificial biological film and preparation method |
| CN101891292A (en) * | 2010-07-27 | 2010-11-24 | 北京师范大学 | Method for removing trace polycyclic aromatic hydrocarbon from water through quick adsorption |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104032400A (en) * | 2014-06-19 | 2014-09-10 | 宜春学院 | Biological enzyme/lignocellulose composite fiber and preparation method thereof |
| CN104032400B (en) * | 2014-06-19 | 2015-12-30 | 宜春学院 | Biology enzyme/lignocellulose composite fibre and preparation method thereof |
| RU2677873C2 (en) * | 2017-07-03 | 2019-01-22 | федеральное государственное бюджетное образовательное учреждение высшего образования "Воронежский государственный университет" (ФГБОУ ВО "ВГУ") | Method of obtaining a heterogeneous drug based on papain |
| CN108251409A (en) * | 2018-01-11 | 2018-07-06 | 山东交通学院 | A kind of complex microorganism guided missile material of absorption degradation greasy dirt and preparation method thereof |
| CN108251409B (en) * | 2018-01-11 | 2020-02-11 | 山东交通学院 | Composite microbial missile material for adsorbing and degrading oil stains and preparation method thereof |
| CN108483678A (en) * | 2018-05-31 | 2018-09-04 | 深圳市碧园环保技术有限公司 | A kind of biological zymophore and laying method for riverbed Water Ecological Recovery |
| CN108743929A (en) * | 2018-06-14 | 2018-11-06 | 四川大学 | A kind of preparation method and purposes of the urase gel micro-ball as urea scavenger |
| CN108743929B (en) * | 2018-06-14 | 2021-07-27 | 四川大学 | Preparation method and application of urease gel microspheres used as urea scavenger |
| CN109012219A (en) * | 2018-09-03 | 2018-12-18 | 安徽工程大学 | A kind of preparation method of fixation in situ laccase ultrafiltration membrane, a kind of processing method of phenol wastewater |
| CN109012219B (en) * | 2018-09-03 | 2021-05-07 | 安徽工程大学 | Preparation method of in-situ immobilized laccase ultrafiltration membrane and treatment method of phenol-containing wastewater |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102716684B (en) | 2014-04-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102716684B (en) | Method for eutrophic water pollution treatment through polylactic acid fiber membrane immobilized compound enzyme technology | |
| Shi et al. | Enhanced anaerobic degradation of nitrogen heterocyclic compounds with methanol, sodium citrate, chlorella, spirulina, and carboxymethylcellulose as co-metabolic substances | |
| CN101915797B (en) | Method for preparing electrostatic spinning immobilized laccase electrode | |
| CN101367575B (en) | Method for resourceful treatment of cadmium polluted wastewater | |
| CN104961313A (en) | Method for enhancing heavy metal stabilization process in sludge anaerobic digestion process | |
| CN103031298A (en) | Immobilized microorganism particle for degrading petroleum in water | |
| CN101407803B (en) | Polylactic acid electrospinning fibre immobilized laccase and preparation thereof | |
| CN110078332A (en) | A method of promoting excess sludge anaerobic fermentation and acid production using modified steel scoria | |
| Zhu et al. | Nitrogen removal performance of anaerobic ammonia oxidation co-culture immobilized in different gel carriers | |
| CN100999728A (en) | Fixed microorganism carrier and preparation process thereof | |
| Geng et al. | Nanocell hybrids for green chemistry | |
| CN104386825B (en) | The method that the pool, a kind of lake water body in-situ is repaired | |
| Wang et al. | Biochar assisted cultivation of Chlorella protothecoides for adsorption of tetracycline and electrochemical study on self-cultured Chlorella protothecoides | |
| CN107381821B (en) | Method for treating overproof mercury in seawater by using microalgae | |
| CN100457899C (en) | Preparation method of chitin nano-fiber composite film immobilized enzyme | |
| CN101096665A (en) | Application of halloysite in enzyme immobilization carrier | |
| WO2024060516A1 (en) | Method for preparing live microorganism-based composite biological material for repairing cr(vi)-polluted water body | |
| CN110085877B (en) | Phenol sewage power generation device based on single-enzyme inorganic composite nanoflower and preparation method and application thereof | |
| CN111019934A (en) | Enzyme immobilized composite material and preparation method thereof | |
| CN102776170A (en) | Preparation method of nanofiber immobilized enzyme in core-shell structure | |
| CN110314638A (en) | It is a kind of for removing the slag Nanoscale Iron support materials of phosphorus element in environment | |
| CN115532813A (en) | Method for restoring heavy metal contaminated soil by taking residual activated sludge as raw material | |
| KR20130018382A (en) | High efficiency conversion of carbon dioxide to bicarbonate using immobilized carbonic anhydrase and method for improving the production of physiologically active materials with photosynthetic microorganisms by using the same | |
| CN102441385A (en) | Method for preparing metal modified active carbon fibers and removing methylene blue in water | |
| CN102730818B (en) | Method for repairing water eutrophication pollution based on urease immobilized nanometer film |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Liu Yanni Inventor before: Niu Junfeng Inventor before: Dai Yunrong Inventor before: Yin Lifeng Inventor before: Ding Shiyuan Inventor before: Bao Yueping Inventor before: Shen Zhenyao |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20170707 Address after: 100037, room 2, building 6, building 2606, building 16, mega street, Beijing, Xicheng District Patentee after: Beijing Pulasen Technology Co. Ltd. Address before: 100875 Beijing city Haidian District Xinjiekou Avenue No. 19 Department of science and technology Patentee before: Beijing Normal University |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20171227 Address after: 100096 Beijing city Haidian District producing up new Garden District 2 building two storey commercial housing 2205 rooms Patentee after: In dalixin (Beijing) Technology Development Co. Ltd. Address before: 100037, room 2, building 6, building 2606, building 16, mega street, Beijing, Xicheng District Patentee before: Beijing Pulasen Technology Co. Ltd. |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20180504 Address after: 523000 4, 402, 403, 404, 8 building, Rui Ying International Science and Technology Innovation Park, 9 University Road, Songshan Lake, Dongguan, Guangdong Patentee after: Guangdong Xinfeng Tatsu Environmental Protection Technology Co., Ltd. Address before: 100096 Haidian District, Beijing, west 2 new apartment building, two floor commercial room 2205. Patentee before: In dalixin (Beijing) Technology Development Co. Ltd. |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20201203 Address after: 100000, 19, Xinjie street, Haidian District, Beijing Patentee after: BEIJING NORMAL University Address before: 402, 403, 404, 4th floor, building 8, Ruiying international science and Technology Innovation Park, No. 9, Daxue Road, Songshan Lake high tech Industrial Zone, Dongguan City, Guangdong Province 523000 Patentee before: GUANGDONG EFENDA ENVIRONMENTAL TECHNOLOGY Co.,Ltd. |