CN102716414A - Anti-tumor medicinal composition containing algal polysaccharide sulfate and preparation method for composition - Google Patents

Abstract

The invention relates to an anti-tumor medicinal composition containing algal polysaccharide sulfate. The composition consists of the following raw medicaments in percentage by weight: 1 to 5 percent of algal polysaccharide sulfate, 0.1 to 1 percent of realgar, 15 to 30 percent of barbed skullcap herb, 15 to 30 percent of rhubarb, and 30 to 45 percent of zedoary. The composition has the advantages that modern biotechnology, pharmacology and Chinese medicinal theory are used as guidance, and the algal polysaccharide sulfate has three anti-tumor effects of promoting cell apoptosis, inhibiting cancer cell angiogenesis and enhancing immunity. The production technology is simple, feasible and mature in technology; and by combining the algal polysaccharide sulfate and the Chinese medicaments, the composition can directly act on a focus and has a good anti-tumor effect, an actual curative effect and low side effect.

Description

A kind of antineoplastic pharmaceutical compositions that contains sulfated fucan and preparation method thereof

Technical field

The present invention relates to technical field of Chinese patent medicine, be specifically related to a kind of antineoplastic pharmaceutical compositions that contains sulfated fucan and preparation method thereof.

Background technology

Tumor be body under various carcinogenic factor effects, some cells of local organization lose the normal regulation to its growth on gene level, cause its clonal abnormality hypertrophy and the neoplasm that forms.Tumor is because due to the electronic cell dysequilibrium.Active oxygen is a kind of material that lacks electronics, fights for electronics everywhere after getting into human body, if seize the electronics of cell protein molecule, makes protein connect side chain generation alkylation, forms distored molecule and carcinogenic.This distortion molecule removes to capture the electronics of neighboring molecule again owing to oneself lack electronics, makes neighboring molecule that distortion also takes place again and carcinogenic.Like this, vicious cycle will form a large amount of distored protein moleculars.When these distored protein molecular breedings were duplicated, gene mutation formed a large amount of cancerous cell, occurs cancer symptoms at last.And when free radical or distortion molecule when having grabbed the electronics of gene, the people will be directly cancer.After human body obtains anion,, unnecessary electronics is arranged, the electronics of cell disappearance can be provided because anion is electronegative, thus the blocking-up vicious cycle, and cancerous cell just can prevent or be suppressed.Nearest result of study shows that also the antioxidation treatment has very important clinical meaning for oncotherapy.Therefore negative aeroion containment zoografting growth of tumor is being applied aspect the control human tumor according to another report.Anion can also be unbalance through regulating the intravital acid base imbalance and the oxidoreduction situation that cause because of malignant tumor, keeps the stability of internal milieu, promotes normal cellular metabolism, alleviates, and eliminates the bad negative interaction of chemotherapy.Especially the physical recovery after the tumor patient chemotherapy had good effect.

Summary of the invention

The object of the present invention is to provide a kind of antineoplastic pharmaceutical compositions that contains sulfated fucan and preparation method thereof, this pharmaceutical composition can have anti-tumor activity by the immunoreation of promoting health, suppresses tumor growth.

A kind of antineoplastic pharmaceutical compositions that contains sulfated fucan, material medicine is formed, by its percetage by weight is: sulfated fucan 1-5%, Realgar 0.1-1%, Herba Scutellariae Barbatae 15-30%, Radix Et Rhizoma Rhei 15-30%, Rhizoma Curcumae 30-45%.

Further, the described antineoplastic pharmaceutical compositions that contains sulfated fucan is an oral formulations.

Contain the method for preparing of the antineoplastic pharmaceutical compositions of sulfated fucan, comprise the steps:

1) take by weighing described each material medicine, Realgar, Herba Scutellariae Barbatae, Radix Et Rhizoma Rhei, Rhizoma Curcumae are pulverized, sieve, subsequent use;

2) the alleged Herba Scutellariae Barbatae of obtaining of step 1, Radix Et Rhizoma Rhei, Rhizoma Curcumae are added alcohol reflux 2 times, 6 hours for the first time, 4 hours for the second time; Gradation is filtered, merging filtrate, decompression recycling ethanol; The resulting product in decompression back is concentrated into the clear paste (80 ℃) of relative density 1.20-1.25, subsequent use;

3) with the resulting clear paste of step 2,60 ℃ down dry, pulverize, sieve, obtain the fine-powdered product, subsequent use;

4) with the resulting fine-powdered product of step 3, with the resulting sulfated fucan of step 1, Realgar powder mixing, both.

Sulfated fucan is the one type of sulfated polysaccharides that from Brown algae, extracts, and mainly contains fucose and sulfate and forms, and is (1-2) or the α-L-fucose-4-sulfuric ester that (1-3) connects of height 3-collateralization.Sulfated fucan has the effect of anticoagulating and lowering blood fat, heavy metal detoxification and anti-HIV aspect medical, causes scientist's great attention, becomes one of marine drug of current focus development.China began sulfated fucan is studied the eighties in 20th century.Sulfated fucan is used for the clinical treatment nephrotic syndrome and early metaphase chronic kidney hypofunction.For chronic kidney hypofunction the reduction blood urea nitrogen is arranged, serum creatinine improves renal function, delays the effect of disease progression.

Realgar is the Ore of mineral Realgar, and matter is soft like mud in the ore deposit, sees that air promptly becomes hard.Acrid in the mouth, warm in nature, poisonous, GUIXIN, liver, spleen, stomach, large intestine channel.Compendium of Material Medica: take by force, put down, tremble with fear, poisonous.Function cures mainly and relieves internal heat anticancerly, and it is relieving convulsion to eliminate the phlegm, and parasite killing is treated skin ulcer, antiinflammatory detumescent.Be used for the carbuncle furuncle, snake bite and insect sting, abdominal pain due to worm stagnation, infantile convulsion, malaria.Main component is arsenic sulfide, and mixing has salts such as small amount of magnesium, ferrum.Have antitumor action, can suppress the growth of transplantability murine sarcoma S-180, and pair cell has corrosiveness.Absorbing the back has spasmolytic, analgesic effect to nerve, and all there is insecticidal action the inside and outside.The water logging agent all has inhibitory action in various degree to staphylococcus aureus, human body tubercule bacillus, Bacillus proteus, green pus coccus and various skin fungus.Can cause after the intestinal absorption tell, rush down, dizzy even faint from fear, chronic poisoning can be damaged the physiological function of liver, kidney.Record in " detailed outline ": Realgar, be to control skin ulcer virus killing key medicine also, and go into the Liver Channel edema caused by disorder of QI, thus liver-wind, irritability, infantile convulsion, sputum, it is dizzy to have a headache, and summer malaria is let out dysentery, gathers all diseases, with it distinguished service is arranged; Can change blood again is water.And necromancer refines to subdue bait, and it says gods and spirits, and is many by its malicious person.

Herba Scutellariae Barbatae acrid in the mouth, hardship, cold in nature, return lung meridian, Liver Channel, kidney channel.The function heat-clearing and toxic substances removing, dissipating blood stasis hemostasis, inducing diuresis to remove edema.Cure mainly: the pyretic toxicity carbuncle, have sore throat, lung abscess, acute appendicitis, scrofula, venom, traumatic injury, haematemesis, epistaxis, stranguria with blood, edema, ascites and cancer.Herb contains carthamin, isocarthamidin, high mountain baicalin, the yellow official seal glycoside of high mountain, cupreol, stearic acid, alkaloid polysaccharide etc.These article contain alkaloid, flavonoid glycoside, steroidal and phenols, tannin etc.Zoopery confirms that Herba Scutellariae Barbatae all has certain inhibitory action to sarcoma 180, ehrlich carcinoma, cerebroma 22 etc.When observing the clinical antitumor curative effect of these Chinese medicines, find, even to treating the late tumor of weary art, also have the effect that improves symptom, suppresses tumor proliferation and prolong patient's life.Experiment also confirms; BANJILIAN has slight inhibitory action to acute granulocyte type leukaemia; Also animal tumors such as W256.U14.S186.EAC, ESC all there is certain inhibitory action, except that antitumaous effect, antibacterial in addition, diuresis, cough-relieving, multiple pharmacological effect such as relieving asthma." Quanzhou book on Chinese herbal medicine ": heat clearing away, detoxifcation, method wind, the blood that looses, circulation of qi promoting, diuretic, collateral dredging, removing blood stasis with potent drugs, pain relieving.Main stranguria with blood for oral administration is spitted blood epistaxis; External smelting venom, carbuncle, furuncle, innominate toxic swelling.

Rhizoma Curcumae acrid in the mouth, hardship, warm in nature, return liver, spleen channel.Function cures mainly: 1) removing blood stasis promoting the circulation of QI to relieve pain: these article effect is with triangular similar, but warm concerted effort is bigger, can treat amenorrhea due to stagnation of blood stomachache, abdominal mass, gather.2) long-pending eliminating stagnation: the pain relieving of these article ability circulation of qi promoting removing food stagnancy is used for eating accumulation, the breast abdominal distention is vexed has a pain.3) removing blood stasis, women's amenorrhea, or the phlegm-damp congestion lump in the abdomen addiction piece that condenses and form.4) promoting the circulation of QI to relieve pain: be suitable for drinking and eating irregularly, the card of gastral cavity abdomen overflow pain.Suitable compatibility, deficient syndrome, the case of a physically strong patient running a high fever all can.Rhizoma Curcumae contains volatile oil; Main constituent is that Rhizoma Curcumae furan ketenes, Borneolum Syntheticum also contain α-and nopinene, lauro lene, limonene, l in the oil, and 8-presses foline, terpinene, isoborneol, Flos Caryophylli alkene, curcumene, Flos Caryophylli alkene epoxide, turmerone, aryl turmerone, curdione and Rhizoma Curcumae enol, isocurcumenol etc."Herbal Supplements", saying: "a Punta Curcuma, black; two Shu, yellow; three waves kill, sweet big drug."" Bencao Tujing " cloud: " Rhizoma Curcumae is given birth to western army and Guangnan Zhu Zhou, modern river, Zhejiang or it is arranged.Give birth to March Seedling afield in.Its stem such as money are big, Senior Two three chis.The leaf Bluish white, long one or two chis, big five cun are come, Po Lei Rhizoma zingiberis miogae.Flower is arranged May, make fringe, yellow, little purple.And luxuriant under root, like the chicken and duck ovum, size seldom like Rhizoma Zingiberis Recens for newspaper ".

The invention has the advantages that with modern biotechnology, pharmacology, Chinese medical theory be guidance, sulfated fucan has three big antitumor actions: the one, and the apoptosis effect; The 2nd, the effect of anticancer blood vessel hyperplasia; The 3rd, immunity potentiation.Simple, the technology maturation of production technology of the present invention, the present invention adopts sulfated fucan and Chinese Medicine and Clavicular, can directly act on focus, and antitumous effect is good, determined curative effect, side effect is little.

The specific embodiment

Embodiment 1

It is following to extract the sulfated fucan technical process:

1) will insert in the ultrasonic response chamber through the Brown algae raw material after clean, drying, the pulverizing, and add acetic acid solution, fully stirring makes even moistening;

2) ultrasonic Treatment applies ultrasonic power, utilizes ultrasound wave, molecular acid and the Brown algae macromolecule polysaccharide synergism between the two, and the glycosidic bond of selective rhizotomy Brown algae macromolecule polysaccharide is realized its controlled degradation;

3) utilize the ultrasonic heating distilling under reduced pressure, get rid of most of acetic acid solution, utilize the acetic acid solution thorough washing, remove the acid of small amount of residual, accomplish the ultrasonic pretreatment of Brown algae;

4) through the Brown algae of microwave pretreatment, add about 5-8 times water and extract, extracting solution concentrates back substep precipitate with ethanol can obtain the water solublity good Algin, fucoidan and the laminaran that are rich in mannuronic acid fragment (rich M);

5) the microwave pretreatment Brown algae raw material residue after process water is carried is handled by alkali digestion technology again, can obtain to be rich in the segmental Algin of guluronic acid.

The preparation process of application of the present invention:

1) take by weighing each material medicine: sulfated fucan 30g, Realgar 5g, Herba Scutellariae Barbatae 525g, Radix Et Rhizoma Rhei 525g, Rhizoma Curcumae 750g, Realgar, Herba Scutellariae Barbatae, Radix Et Rhizoma Rhei, Rhizoma Curcumae are pulverized, sieve, subsequent use;

2) the alleged Herba Scutellariae Barbatae of obtaining of step 1, Radix Et Rhizoma Rhei, Rhizoma Curcumae are added alcohol reflux 2 times, 6 hours for the first time, 4 hours for the second time; Gradation is filtered, merging filtrate, decompression recycling ethanol; The resulting product in decompression back is concentrated into the clear paste (80 ℃) of relative density 1.20-1.25, subsequent use;

3) with the resulting clear paste of step 2,60 ℃ down dry, pulverize, sieve, obtain the fine-powdered product, subsequent use;

4) with the resulting fine-powdered product of step 3, with the resulting sulfated fucan of step 1, Realgar powder mixing, both.

The above; Be merely the preferable specific embodiment of the present invention; But protection scope of the present invention is not limited thereto; Any technical staff who is familiar with the present technique field is equal to replacement or change according to technical scheme of the present invention and inventive concept thereof in the technical scope that the present invention discloses, all should be encompassed within protection scope of the present invention.

Claims (3)

1. an antineoplastic pharmaceutical compositions that contains sulfated fucan is characterized in that, material medicine is formed, by its percetage by weight is: sulfated fucan 1-5%, Realgar 0.1-1%, Herba Scutellariae Barbatae 15-30%, Radix Et Rhizoma Rhei 15-30%, Rhizoma Curcumae 30-45%.

2. the antineoplastic pharmaceutical compositions that contains sulfated fucan as claimed in claim 1 is characterized in that, described pharmaceutical composition is an oral formulations.

3. according to claim 1 or claim 2 the method for preparing of the antineoplastic pharmaceutical compositions that contains sulfated fucan comprises the steps:

1) take by weighing described each material medicine, Realgar, Herba Scutellariae Barbatae, Radix Et Rhizoma Rhei, Rhizoma Curcumae are pulverized, sieve, subsequent use;

2) the alleged Herba Scutellariae Barbatae of obtaining of step 1, Radix Et Rhizoma Rhei, Rhizoma Curcumae are added alcohol reflux 2 times, 6 hours for the first time, 4 hours for the second time; Gradation is filtered, merging filtrate, decompression recycling ethanol; The resulting product in decompression back is concentrated into the clear paste (80 ℃) of relative density 1.20-1.25, subsequent use;

3) with the resulting clear paste of step 2,60 ℃ down dry, pulverize, sieve, obtain the fine-powdered product, subsequent use;

4) with the resulting fine-powdered product of step 3, with the resulting sulfated fucan of step 1, Realgar powder mixing, both.