CN102690322A - Method for decolorizing silkworm chrysalis protein enzymatic hydrolysis peptide - Google Patents
Method for decolorizing silkworm chrysalis protein enzymatic hydrolysis peptide Download PDFInfo
- Publication number
- CN102690322A CN102690322A CN2012101840873A CN201210184087A CN102690322A CN 102690322 A CN102690322 A CN 102690322A CN 2012101840873 A CN2012101840873 A CN 2012101840873A CN 201210184087 A CN201210184087 A CN 201210184087A CN 102690322 A CN102690322 A CN 102690322A
- Authority
- CN
- China
- Prior art keywords
- pupa albumen
- percent
- retention rate
- oxidation peptide
- albumen enzymolysis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention relates to a method for decolorizing silkworm chrysalis protein enzymatic hydrolysis peptide. The method comprises the following steps of: adjusting pH of a 1 percent silkworm chrysalis protein enzymatic hydrolysis peptide solution to be 8, adding 30g of powdery activated carbon and activated clay composite decolorizer in a ratio of 1:7 into each liter of the solution, placing in an oscillatory type water bath kettle at the temperature of 30 DEG C, adjusting the rotation speed to be 200r/min, decolorizing for 30 minutes, and centrifuging by using a disk centrifuge at the speed of 5,000 to 6,000r/min for 30 minutes, wherein the decolorization rate is 70.39 percent, the nitrogen retention rate is 91.14 percent, and the reducing power retention rate is 86.79 percent. The process is simple and easy to operate, the treatment process is mild, and the method can be widely applied to health food industry in which silkworm chrysalis polypeptide and other bioactive peptides are produced.
Description
Technical field
The present invention relates to a kind of decoloring method of pupa albumen enzymolysis anti-oxidation peptide.
Background technology
Anti-oxidation peptide is the focus of studying in recent years, discovers in vegetable-protein, animal proteinum and the microbial proteinous and can hydrolysis isolate biologically active peptidess such as blood pressure lowering peptide, immunomodulatory peptides, antibacterial peptide, OPIOIDS peptide and anti-oxidation peptide.Wherein anti-oxidation peptide can reach anti-oxidant function through reducing various radicals, inhibition lipid peroxidation and chelated metal ions in vivo.Existing research obtains anti-oxidation peptide from proteolysiss such as soybean, corn, peanut, fish-skins.
China is sericulture big country, produces 650000 tons of silk cocoons per year, accounts for 70% of the total cocoon amount in the world.As a sub product of putting down silk industry, silkworm chrysalis processing and utilization rate is extremely low, except that minority through oven dry is pulverized as nutritional additive, mostly much go out of use in addition as feed even fertilizer, cause the huge waste of resource.Pupa albumen content is high, the amino acid A wide selection of colours and designs, is a kind of good anti-oxidation peptide source.Yet the pupa albumen hydrolyzed solution has Vandyke brown, on color and luster, is difficult for being accepted by people as medicine or foodstuff additive, therefore must be to its processing of decolouring.At present research silkworm chrysalis enzymolyzing liquid or other bioactive peptide decoloring methods mainly adopt activated carbon method, dioxygen water law, organic solvent method, but problem such as these methods exist the loss of proteins rate high, and the bioactive peptide loss of activity is serious.
Summary of the invention
The object of the present invention is to provide a kind of decoloring method of pupa albumen enzymolysis anti-oxidation peptide.This method has improved percent of decolourization effectively, has kept nitrogen retention rate and reducing power retention rate simultaneously, can be widely used in the protective foods industry that silkworm chrysalis polypeptide and other bioactive peptides are produced.
For achieving the above object, following technical scheme is adopted in invention:
A kind of decoloring method of pupa albumen enzymolysis anti-oxidation peptide; The concrete steps that it is characterized in that this method are: the pH that regulates pupa albumen enzymolysis anti-oxidation peptide solution is: 7～9, add composite decoloring agent, and the mass volume ratio of this composite decoloring agent and pupa albumen enzymolysis anti-oxidation peptide is: 20～40g/L; In the oscillatory type water-bath; Adjust the temperature to 20～50 ℃, rotating speed to 150～250 r/min is behind decolouring 20～30 min; Spinning, gained supernatant are the pupa albumen enzymolysis anti-oxidation peptide of handling through decolouring.The pH of above-mentioned pupa albumen enzymolysis anti-oxidation peptide solution is 8.
Above-mentioned composite decoloring agent be Powdered Activated Carbon and atlapulgite be 1 by mass ratio: the mixture of (3～9).
Above-mentioned composite decoloring agent addition is 30 g/L of pupa albumen enzymolysis anti-oxidation peptide quality.
Above-mentioned bleaching temperature is 30 ℃.
Decoloring method technology of the present invention is simple, operation is prone to row, and treating processes is gentle, and percent of decolourization is 70.39%, and the nitrogen retention rate is 91.14%, and the reducing power retention rate is 86.79%.Can be widely used in the protective foods industry that silkworm chrysalis polypeptide and other bioactive peptides are produced.
Embodiment
To combine the embodiment form below
, foregoing of the present invention will be described further.
Embodiment one: the selection of discoloring agent
1% pupa albumen enzymolysis anti-oxidation peptide solution is regulated pH7, adds each 20 g/L of Powdered Activated Carbon and atlapulgite then respectively; Put into the oscillatory type water-bath, 30 ℃ of temperature are regulated rotating speed 200 r/min; Decolour behind 30 min centrifugal 30 min of disk centrifugal separator 5000-6000r/min.Get supernatant and survey its percent of decolourization, nitrogen retention rate, reducing power retention rate.Result such as table 1.
Can know that by table 1 Powdered Activated Carbon has good decolorizing effect, but in decolouring, also lose active polypeptide, and then influenced the anti-oxidant activity of supernatant.And atlapulgite has kept nitrogen content and reducing power ability preferably on the basis of certain decolorizing effect.
The decolorizing effect of table 1 Powdered Activated Carbon and atlapulgite
| ? | Powdered Activated Carbon | Atlapulgite |
| Percent of decolourization (%) | 90.27 | 52.52 |
| Nitrogen retention rate (%) | 14.20 | 94.86 |
| Reducing power retention rate (%) | 12.95 | 91.58 |
Embodiment two: the proportioning of composite decoloring agent
1% pupa albumen enzymolysis anti-oxidation peptide solution; Transfer pH7, add the Powdered Activated Carbon and atlapulgite composite decoloring agent 20 g/L of different ratios respectively, put into the oscillatory type water-bath; 30 ℃ of temperature; Turn fast 200 r/min, behind 30 min that decolour, centrifugal 30 min of disk centrifugal separator 5000-6000 r/min.Get supernatant and survey its percent of decolourization, nitrogen retention rate, reducing power retention rate.Result such as table 2.
Can be known that by table 2 along with the minimizing of Powdered Activated Carbon ratio, the increase of atlapulgite ratio, percent of decolourization decreases, nitrogen retention rate and reducing power retention rate significantly improve.Powdered Activated Carbon: decolorizing effect was better when the atlapulgite ratio was 1:7, and still can keep nitrogen retention rate and reducing power retention rate preferably.
The decolorizing effect of the composite decoloring agent of the different proportionings of table 2
Embodiment three: the decolorization condition list factor of composite decoloring agent is optimized
1% pupa albumen enzymolysis anti-oxidation peptide solution; Transfer pH 5-9, add Powdered Activated Carbon and atlapulgite composite decoloring agent (1:7) 10-50 g/L, put into the oscillatory type water-bath; Temperature 20-100 ℃; Regulate rotating speed to 200 r/min, behind 30 min that decolour, use centrifugal 30 min of disk centrifugal separator 5000-6000 r/min.Get supernatant and survey its percent of decolourization, nitrogen retention rate, reducing power retention rate.The result is like table 3-5.
Can know that by table 3 along with pH increases, percent of decolourization reduces, but nitrogen retention rate and reducing power retention rate improve significantly.
Can know that by table 4 percent of decolourization, nitrogen retention rate and reducing power retention rate are along with the increase of temperature changes little.
Can know that by table 5 increase with addition, percent of decolourization improves, but nitrogen retention rate and reducing power retention rate reduce significantly.
Table 3 pH is to the influence of decolorizing effect
| pH | 5 | 6 | 7 | 8 | 9 |
| Percent of decolourization (%) | 73.69 | 70.10 | 68.78 | 62.33 | 57.55 |
| Nitrogen retention rate (%) | 59.37 | 69.66 | 89.80 | 94.07 | 95.39 |
| Reducing power retention rate (%) | 49.66 | 62.50 | 81.53 | 90.48 | 93.00 |
Annotate: 30 ℃ of temperature, discoloring agent adds concentration 20g/L
Table 4 temperature is to the influence of decolorizing effect
| Temperature (℃) | 20 | 40 | 60 | 80 | 100 |
| Percent of decolourization (%) | 68.89 | 69.87 | 65.69 | 67.11 | 70.39 |
| Nitrogen retention rate (%) | 90.64 | 91.15 | 91.47 | 91.85 | 90.06 |
| Reducing power retention rate (%) | 78.94 | 81.34 | 81.84 | 82.67 | 80.42 |
Annotate: pH7, discoloring agent adds concentration 20g/L
Table 5 composite decoloring agent addition is to the influence of decolorizing effect
| Addition (g/L) | 10 | 20 | 30 | 40 | 50 |
| Percent of decolourization (%) | 50.19 | 68.26 | 74.81 | 80.00 | 85.66 |
| Nitrogen retention rate (%) | 98.19 | 90.14 | 86.15 | 83.20 | 76.52 |
| Reducing power retention rate (%) | 96.15 | 80.26 | 75.40 | 70.90 | 62.81 |
Annotate: pH7,30 ℃ of temperature
Embodiment four: the decolorization condition orthogonal optimization of composite decoloring agent
Table 6 composite decoloring agent quadrature level of factor table
| Factor | A(pH) | B (temperature/℃) | C (addition/g/L) |
| 1 | 7 | 30 | 20 |
| 2 | 8 | 40 | 30 |
| 3 | 9 | 50 | 40 |
Table 7 composite decoloring agent Orthogonal experiment results table
Drawn by table 7: the impact effect to percent of decolourization is C>A>B, percent of decolourization is best is combined as A1B1C3, i.e. pH7,30 ℃ of temperature, the composite decoloring agent addition is 40 g/L.Impact effect to the nitrogen retention rate is A>C>B, the nitrogen retention rate is best is combined as A3B2C1, i.e. pH9, temperature is 40 ℃, the composite decoloring agent addition is 20 g/L.Impact effect to the reducing power retention rate is A>C>B, the reducing power retention rate is best is combined as A3B1C1, i.e. pH9,30 ℃ of temperature, the composite decoloring agent addition is 20 g/L.
Take all factors into consideration; Retained nitrogen retention rate and reducing power retention rate preferably when improving percent of decolourization, decolorization condition is: pH8, temperature is 30 ℃; Composite decoloring agent (Powdered Activated Carbon: atlapulgite is 1:7) addition is 30 g/L; The percent of decolourization of this moment is 70.39%, and the nitrogen retention rate is 91.14%, and the reducing power retention rate is 86.79%.
Claims (5)
1. the decoloring method of a pupa albumen enzymolysis anti-oxidation peptide; The concrete steps that it is characterized in that this method are: the pH that regulates pupa albumen enzymolysis anti-oxidation peptide solution is: 7～9, add composite decoloring agent, and the mass volume ratio of this composite decoloring agent and pupa albumen enzymolysis anti-oxidation peptide is: 20～40g/L; In the oscillatory type water-bath; Adjust the temperature to 20～50 ℃, rotating speed to 150～250 r/min is behind decolouring 20～30 min; Spinning, gained supernatant are the pupa albumen enzymolysis anti-oxidation peptide of handling through decolouring.
2. the decoloring method of pupa albumen enzymolysis anti-oxidation peptide according to claim 1 is characterized in that the pH of described pupa albumen enzymolysis anti-oxidation peptide solution is 8.
3. the decoloring method of pupa albumen enzymolysis anti-oxidation peptide according to claim 1, it is characterized in that described composite decoloring agent be Powdered Activated Carbon and atlapulgite be 1 by mass ratio: the mixture of (3～9).
4. according to the decoloring method of claim 1 or 3 pupa albumen enzymolysis anti-oxidation peptides, it is characterized in that described composite decoloring agent addition is 30 g/L of pupa albumen enzymolysis anti-oxidation peptide quality.
5. according to the decoloring method of claim 1 pupa albumen enzymolysis anti-oxidation peptide, it is characterized in that described bleaching temperature is 30 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101840873A CN102690322A (en) | 2012-06-06 | 2012-06-06 | Method for decolorizing silkworm chrysalis protein enzymatic hydrolysis peptide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101840873A CN102690322A (en) | 2012-06-06 | 2012-06-06 | Method for decolorizing silkworm chrysalis protein enzymatic hydrolysis peptide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102690322A true CN102690322A (en) | 2012-09-26 |
Family
ID=46856074
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012101840873A Pending CN102690322A (en) | 2012-06-06 | 2012-06-06 | Method for decolorizing silkworm chrysalis protein enzymatic hydrolysis peptide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102690322A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103468601A (en) * | 2013-06-27 | 2013-12-25 | 上海大学 | Method used for increasing cold stress resistance of lactococcus lactis |
| CN103804466A (en) * | 2013-12-20 | 2014-05-21 | 广西大学 | Decolorizing method of silkworm chrysalis proteolysis antioxidative peptide |
| CN108218958A (en) * | 2017-12-27 | 2018-06-29 | 浙江省农业科学院 | Polypeptide SVLGTGC with anti-oxidation function and application thereof |
| CN110214755A (en) * | 2019-07-02 | 2019-09-10 | 上海大学 | A kind of cultural method of selenium-rich silkworm chrysalis |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1686371A (en) * | 2005-03-30 | 2005-10-26 | 王伟 | Bencao serum health are oil and its preparation method |
| CN101305974A (en) * | 2008-07-11 | 2008-11-19 | 广东新大地生物科技有限公司 | Camellia oil preparation method for cosmetics |
| CN101691520A (en) * | 2009-10-14 | 2010-04-07 | 北京市科威华食品工程技术有限公司 | Walnut oil refining method |
| CN101921254A (en) * | 2010-08-12 | 2010-12-22 | 江苏省中国科学院植物研究所 | Method for extracting natural vitamin E from blackberry seeds |
-
2012
- 2012-06-06 CN CN2012101840873A patent/CN102690322A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1686371A (en) * | 2005-03-30 | 2005-10-26 | 王伟 | Bencao serum health are oil and its preparation method |
| CN101305974A (en) * | 2008-07-11 | 2008-11-19 | 广东新大地生物科技有限公司 | Camellia oil preparation method for cosmetics |
| CN101691520A (en) * | 2009-10-14 | 2010-04-07 | 北京市科威华食品工程技术有限公司 | Walnut oil refining method |
| CN101921254A (en) * | 2010-08-12 | 2010-12-22 | 江苏省中国科学院植物研究所 | Method for extracting natural vitamin E from blackberry seeds |
Non-Patent Citations (3)
| Title |
|---|
| 敬承衡等: "蚕蛹蛋白脱色、脱臭的研究", 《云南师范大学学报》 * |
| 粟晖等: "蚕蛹蛋白水解液的脱色条件研究", 《粮油加工》 * |
| 陈亮: "家蚕蛹蛋白精制工艺的研究", 《中国优秀硕士学位论文全文数据库》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103468601A (en) * | 2013-06-27 | 2013-12-25 | 上海大学 | Method used for increasing cold stress resistance of lactococcus lactis |
| CN103804466A (en) * | 2013-12-20 | 2014-05-21 | 广西大学 | Decolorizing method of silkworm chrysalis proteolysis antioxidative peptide |
| CN108218958A (en) * | 2017-12-27 | 2018-06-29 | 浙江省农业科学院 | Polypeptide SVLGTGC with anti-oxidation function and application thereof |
| CN108218958B (en) * | 2017-12-27 | 2020-04-17 | 浙江省农业科学院 | Polypeptide SVLGTGC with antioxidant function and application thereof |
| CN110214755A (en) * | 2019-07-02 | 2019-09-10 | 上海大学 | A kind of cultural method of selenium-rich silkworm chrysalis |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US9974321B2 (en) | Fish protein oligopeptide with low allergenicity and slight fishiness and industrial preparation method and application thereof | |
| CN104250285B (en) | Pseudosciaena crocea flesh antioxidative peptide and preparation method and use thereof | |
| CN103992385A (en) | Pseudosciaena crocea swim bladder antioxidant collagen peptide and preparation method and application thereof | |
| CN102599335B (en) | Method for preparing compound microorganism fermented forage feed | |
| CN104017850B (en) | Method and the application of the little peptide of Paeonia suffruticosa of the little peptide of Paeonia suffruticosa prepared by a kind of seed of Flos Moutan grouts | |
| CN102690322A (en) | Method for decolorizing silkworm chrysalis protein enzymatic hydrolysis peptide | |
| CN107821989A (en) | A kind of glycosylation process for improving class PSE Fresh Grade Breast fribrillin Gelatin Qualities | |
| CN103082160A (en) | High-efficiency deodorization and low-nutritional content loss method of conducting wall breaking and deodorization to spirulina | |
| CN104694606B (en) | A kind of method that small-molecular peptides are prepared with complex enzyme for hydrolyzing Oyster Protein | |
| CN106538845A (en) | A kind of chelating ferrous preparation method of marine protein peptide | |
| CN104250286B (en) | Navodon septentrionalis fish-skin antioxidant collagen peptide and preparation method and use thereof | |
| CN103361393A (en) | Preparation method of andrias oligopeptide | |
| CN106359839A (en) | Extraction method of oyster peptides | |
| CN109371088A (en) | A kind of preparation method of sea cucumber active peptide | |
| CN104450629A (en) | Chinese soft-shell turtle aeromonas phage and application thereof | |
| CN104745665A (en) | Collagen peptide with function of promoting bone growth as well as preparation method and application thereof | |
| CN104311645B (en) | Spirulina polypeptide P1 and its application with bacteriostatic activity | |
| CN102925361B (en) | AFB1 degrading bacterium and degrading enzyme | |
| CN106538844B (en) | Preparation method of marine organism protein peptide chelated copper | |
| CN103421873A (en) | Preparation method for bacteriostatic seabuckthorn seed polypeptide | |
| CN102676475A (en) | Method for extracting muramidase from egg white | |
| CN104099393B (en) | Seed of Fructus Hippophae polypeptide with bacteriostasis and its preparation method and application | |
| CN103695513B (en) | A kind of method improving yield of soybean peptide with low molecular weight | |
| CN105200107B (en) | The extracting method of Onchidium struma muscle crude protein | |
| CN102277403B (en) | Production technology of yellow wine lees proteins by enzymatic extraction |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20120926 |