CN102688153A - Blank liposome prepared by compounding phospholipid and preparation method thereof - Google Patents
Blank liposome prepared by compounding phospholipid and preparation method thereof Download PDFInfo
- Publication number
- CN102688153A CN102688153A CN2012101822381A CN201210182238A CN102688153A CN 102688153 A CN102688153 A CN 102688153A CN 2012101822381 A CN2012101822381 A CN 2012101822381A CN 201210182238 A CN201210182238 A CN 201210182238A CN 102688153 A CN102688153 A CN 102688153A
- Authority
- CN
- China
- Prior art keywords
- phospholipid
- liposome
- blank liposome
- water
- alcohol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Cosmetics (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention discloses a blank liposome prepared by compounding phospholipid, which takes on faint yellow gel shape, and comprises the components by weight percent: 1-10% of soya bean lecithin, 1-10% of hydrogenated phospholipid, 5-20% of alcohol and the balance of deionized water, wherein the alcohol is selected from at least one in ethanol, propylene glycol, glycerol and 1, 3-butanediol. The blank liposome has the gel-shaped appearance, and is good in stability; the preparation method is simple, controllable and good in repeatability; and the blank liposome can be mixed with water according to any proportion, thus being applied to the preparation of daily chemical products.
Description
Technical field
The invention belongs to the carrier system technical field in the cosmetics technology of preparing, relate to a kind of liposome and preparation method thereof, particularly a kind of blank liposome and preparation method thereof.
Background technology
Liposome has the bilayer microcapsule that is similar to biofilm structure; The phospholipid and the formed lipoid of cholesterol that constitute bilayer are divided into hydrophilic head and lipophilic afterbody two parts; In the forming process of liposome; The film forming surfaces externally and internally layer of hydrophilic head shape, and oil loving afterbody is in the centre of film.This structure of liposome makes it can carry various hydrophilic, hydrophobic and amphipathic materials, and these materials are wrapped into the liposome interior water, or inserts the inner lipid bilayer, or absorption is connected the surface of liposome.
The mechanism of action of liposome cosmetics and the mechanism of percutaneous dosing are similar.People's skin is that cell membrane is impermeable to the macromole bioactive substance by the fine and close cellularity of one deck, make cosmetics particularly the active component in the skin care item be difficult to bring into play effect through physiological process such as infiltration, absorptions.The class membrane structure of liposome make it can with the human epidermal cell fusion, increase the flowability of epidermis cell film; Add size and liquid state and deformability preferably; Not only help liposome and penetrate epidermal barrier, its entrapped effective ingredient can be got in the skin corium, and can activate the biochemical reaction relevant with film; Promote the epidermis cell substance metabolism, make skin more pliable and tougher, flexible.And the liposome cosmetics have the good moisture preserving effect to skin, even under the form of zero load, liposome still can play activating cell and keep water function at deep skin.Liposome infiltrates skin and in horny layer, gathers; After having an effect with intercellular fat layer; The mobile of its pair film constantly increases; Unsaturated fatty acid in the phospholipid and hydrophilic group head end are organized and are constantly decomposed in the horny layer, the inequality that the subcutaneous tissue cell that unsaturated fatty acid on the one hand can replenish for some reason to be caused is arranged; Can reduce superabundant fats and the toxin deposition on skin on the other hand, the hydrophilic group of phospholipid can the cornified tissue of moistening simultaneously.Comprehensive through above two kinds of effects, liposome improves significantly to the roughness of skin.Compare with the skin care item that do not use liposome, its skin protection is renderd a service strong several times to tens times.Therefore, the someone claims that liposome is skin " proton ".
In sum, liposome has good moisture preserving and skin-care effect, even blank liposome also has great benefit to skin, is used in the cosmetics, can increase the effect of cosmetics greatly.
Summary of the invention
The object of the invention is the blank liposome that a kind of stability is high, the compatibility is good is provided, and is mixed by two kinds of phospholipid, has improved the content of phospholipid in the blank liposome, and the blank liposome of preparing is gel, and outward appearance is tempting.
In order to solve these problems of the prior art, technical scheme provided by the invention is:
A kind of by the composite blank liposome of processing of phospholipid, it is characterized in that composition is counted by its percentage by weight in the said liposome:
Soybean lecithin 1% ~ 10%;
Hydrogenated phospholipid 1% ~ 10%;
Alcohol 5% ~ 20%;
All the other are deionized water;
Wherein the content of the phosphatidylcholine of soybean lecithin (PC) is between 40% ~ 70%, and the PC content of hydrogenated phospholipid is between 20% ~ 50%; Said alcohol is selected from least a following chemical compound: ethanol, propylene glycol, glycerol, 1,3 butylene glycol.
Another object of the present invention is to provide a kind of method for preparing described blank liposome, it is characterized in that said method comprising the steps of:
(1) presses formula proportion weighing hydrogenated phospholipid and water, with hydrogenated phospholipid and water mixing post-heating and stir and obtain water;
(2) press formula proportion weighing soybean lecithin and alcohol, soybean lecithin and alcohol heating mixing are obtained liquid oil phase;
(3) in 50-70 ℃ liquid oil phase, add 50-70 ℃ water, mix homogeneously adds the high pressure homogenizer rotation homogenizing several times that are preheated to 50-70 ℃ again, and cool to room temperature can obtain described blank liposome.
Preferably, the pressure setting of said method mesohigh homogenizer is in the 30-60MPa scope.
Preferably, count by its percentage by weight by composition in the composite blank liposome of processing of phospholipid:
Soybean lecithin 5.0～10%;
Hydrogenated phospholipid 5.0～10%;
Alcohol 5.0～15%;
All the other are deionized water;
Wherein the phosphatidylcholine of soybean lecithin (PC) content is between 40% ~ 70%, and the PC content of hydrogenated phospholipid is between 20% ~ 50%; Said alcohol is selected from following at least a chemical compound: ethanol, propylene glycol, glycerol, 1,3 butylene glycol.
Said blank liposome is made up of 2 kinds of phospholipid, and the said liposome outward appearance in preparation back is the light yellow gel shape.Composition is by weight percentage in this system: soybean lecithin 1% ~ 10%, and hydrogenated phospholipid 1% ~ 10%, alcohol 5% ~ 20%, all the other are deionized water; Said alcohol is selected from following at least a material: ethanol, propylene glycol, glycerol, 1,3 butylene glycol.This blank liposome outward appearance is gel, has good stable property, the method for preparing simple controllable, and good reproducibility mixes with the water arbitrary proportion, can be applied in the preparation of daily chemical products.
With respect to scheme of the prior art, advantage of the present invention is:
The preparation of blank liposome of the present invention is controlled.Can regulate and control through regulating conditions such as prescription, high pressure homogenize cycle-index.
Method for preparing of the present invention is simple, good reproducibility, and the gel liposome outward appearance of preparing is tempting.Can it be added in the cosmetic formulations, or directly use.
Description of drawings
Below in conjunction with accompanying drawing and embodiment the present invention is further described:
Fig. 1 is the method for preparing flow chart of the present composition.
The specific embodiment
Below in conjunction with specific embodiment such scheme is further specified.These embodiment are used to the present invention is described and are not limited to limit scope of the present invention.The implementation condition that adopts among the embodiment can be done further adjustment according to the condition of concrete producer, and not marked implementation condition is generally the condition in the normal experiment.
The preparation of embodiment 1 blank liposome
Composite formula
As shown in Figure 1, the preparation process of this embodiment is following:
1. take by weighing 5.0 gram hydrogenated phospholipids and 85.0 gram deionized waters, with hydrogenated phospholipid and water mixings post-heating to 50 ℃ also stirring obtain water.
2. take by weighing 5.0 gram soybean lecithins and 5.0 gram ethanol, soybean lecithin and ethanol are heated to 50 ℃ of mixings obtain oil phase.
3. in oil phase, add water, mix homogeneously adds and is preheated to the high pressure homogenizer rotation homogenizing 3 times that 50 ℃, pressure are 40MPa, obtains blank liposome.
4. be cooled to room temperature and get the gel blank liposome, it is 180nm that this dispersion liquid records its particle diameter through photon correlation spectroscopy (PCS).
The preparation of embodiment 2 blank liposomes
Composite formula
Preparation process:
1. take by weighing 5.0 gram hydrogenated phospholipids and 75.0 gram deionized waters, with hydrogenated phospholipid and water mixings post-heating to 50 ℃ also stirring obtain water.
2. take by weighing 10.0 gram soybean lecithins and 10.0 gram ethanol, soybean lecithin and ethanol are heated to 50 ℃ of mixings obtain oil phase.
3. in oil phase, add water, mix homogeneously adds and is preheated to the high pressure homogenizer rotation homogenizing 3 times that 50 ℃, pressure are 50MPa, obtains blank liposome.
4. be cooled to room temperature and get the gel blank liposome, it is 148nm that this dispersion liquid records its particle diameter through photon correlation spectroscopy (PCS);
The preparation of embodiment 3 blank liposomes
Composite formula
Preparation process:
1. take by weighing 7.0 gram hydrogenated phospholipids and 85.0 gram deionized waters, with hydrogenated phospholipid and water mixings post-heating to 65 ℃ also stirring obtain water.
2. take by weighing 3.0 gram soybean lecithins and 5.0 gram propylene glycol, soybean lecithin and ethanol are heated to 65 ℃ of mixings obtain oil phase.
3. in oil phase, add water, mix homogeneously adds and is preheated to the high pressure homogenizer rotation homogenizing 3 times that 65 ℃, pressure are 50MPa, obtains blank liposome.
4. be cooled to room temperature and get the gel blank liposome, it is 165nm that this dispersion liquid records its particle diameter through photon correlation spectroscopy (PCS);
The preparation of embodiment 4 blank liposomes
Composite formula
Preparation process:
1. take by weighing 3.0 gram hydrogenated phospholipids, 5.0 gram 1,3 butylene glycols, 5.0 gram glycerol and 75.0 gram deionized waters, it is mixed post-heating to 50 ℃ and stirs obtaining water.
2. take by weighing 7.0 gram soybean lecithins and 5.0 gram propylene glycol, soybean lecithin and ethanol are heated to 50 ℃ of mixings obtain oil phase.
3. in oil phase, add water, mix homogeneously adds and is preheated to the high pressure homogenizer rotation homogenizing 3 times that 50 ℃, pressure are 50MPa, obtains blank liposome.
4. be cooled to room temperature and get the gel blank liposome, it is 172nm that this dispersion liquid records its particle diameter through photon correlation spectroscopy (PCS);
Above-mentioned instance only is explanation technical conceive of the present invention and characteristics, and its purpose is to let the people who is familiar with this technology can understand content of the present invention and enforcement according to this, can not limit protection scope of the present invention with this.All equivalent transformations that spirit is done according to the present invention or modification all should be encompassed within protection scope of the present invention.
Claims (3)
1. one kind by the composite blank liposome of processing of phospholipid, it is characterized in that composition is counted by its percentage by weight in the said liposome:
Soybean lecithin 1% ~ 10%;
Hydrogenated phospholipid 1% ~ 10%;
Alcohol 5% ~ 20%;
All the other are deionized water;
Wherein the content of the phosphatidylcholine of soybean lecithin (PC) is between 40% ~ 70%, and the PC content of hydrogenated phospholipid is between 20% ~ 50%; Said alcohol is selected from least a following chemical compound: ethanol, propylene glycol, glycerol, 1,3 butylene glycol.
2. method for preparing the described blank liposome of claim 1 is characterized in that said method comprising the steps of:
(1) presses formula proportion weighing hydrogenated phospholipid and water, with hydrogenated phospholipid and water mixing post-heating and stir and obtain water;
(2) press formula proportion weighing soybean lecithin and alcohol, soybean lecithin and alcohol heating mixing are obtained liquid oil phase;
(3) in 50-70 ℃ liquid oil phase, add 50-70 ℃ water, mix homogeneously adds the high pressure homogenizer rotation homogenizing several times that are preheated to 50-70 ℃ again, and cool to room temperature can obtain described blank liposome.
3. according to the method for claim 2, the pressure setting that it is characterized in that said method mesohigh homogenizer is in the 30-60MPa scope.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101822381A CN102688153A (en) | 2012-06-05 | 2012-06-05 | Blank liposome prepared by compounding phospholipid and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101822381A CN102688153A (en) | 2012-06-05 | 2012-06-05 | Blank liposome prepared by compounding phospholipid and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102688153A true CN102688153A (en) | 2012-09-26 |
Family
ID=46854002
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012101822381A Pending CN102688153A (en) | 2012-06-05 | 2012-06-05 | Blank liposome prepared by compounding phospholipid and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102688153A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105078776A (en) * | 2015-09-01 | 2015-11-25 | 赵洪波 | Cosmetic active matter carrier coating and preparation method thereof |
| CN111132753A (en) * | 2017-09-04 | 2020-05-08 | 一丸自然美建有限公司 | pH-sensitive liposome and method for producing same |
| CN111374947A (en) * | 2018-12-25 | 2020-07-07 | 太阳星光齿磨公司 | Liposome, liposome-containing composition, method for producing liposome, and method for improving thermal stability of retinol |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19922193A1 (en) * | 1999-05-12 | 2000-11-16 | Frank Sonnenberg | Stable, solubilizer-free, water-dilutable essential oil concentrate formulation, useful e.g. in pharmaceutical, cosmetic or food applications, comprising liposomes based on hydrogenated lecithin |
| CN101027065A (en) * | 2004-06-15 | 2007-08-29 | 陈献 | Phospholipid compositions and methods for their preparation and use |
| CN101406455A (en) * | 2008-11-28 | 2009-04-15 | 南京中医药大学 | Strychnine composite phospholipid liposome, and preparation method thereof and use in pharmacy |
| CN101653415A (en) * | 2009-09-01 | 2010-02-24 | 沈阳顺旺动物药业有限公司 | Florfenicol liposome preparation and preparation method thereof |
| CN102210870A (en) * | 2011-06-09 | 2011-10-12 | 南京中医药大学 | Liposome composite phospholipid capable of adjusting phase-transition temperature and application thereof |
| KR20120009995A (en) * | 2010-07-23 | 2012-02-02 | (주)나노팜 | Mixed micells improving stability of retinyl palmitates, preparation method thereof and cosmetic compositions containing the same |
-
2012
- 2012-06-05 CN CN2012101822381A patent/CN102688153A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19922193A1 (en) * | 1999-05-12 | 2000-11-16 | Frank Sonnenberg | Stable, solubilizer-free, water-dilutable essential oil concentrate formulation, useful e.g. in pharmaceutical, cosmetic or food applications, comprising liposomes based on hydrogenated lecithin |
| CN101027065A (en) * | 2004-06-15 | 2007-08-29 | 陈献 | Phospholipid compositions and methods for their preparation and use |
| CN101406455A (en) * | 2008-11-28 | 2009-04-15 | 南京中医药大学 | Strychnine composite phospholipid liposome, and preparation method thereof and use in pharmacy |
| CN101653415A (en) * | 2009-09-01 | 2010-02-24 | 沈阳顺旺动物药业有限公司 | Florfenicol liposome preparation and preparation method thereof |
| KR20120009995A (en) * | 2010-07-23 | 2012-02-02 | (주)나노팜 | Mixed micells improving stability of retinyl palmitates, preparation method thereof and cosmetic compositions containing the same |
| CN102210870A (en) * | 2011-06-09 | 2011-10-12 | 南京中医药大学 | Liposome composite phospholipid capable of adjusting phase-transition temperature and application thereof |
Non-Patent Citations (6)
| Title |
|---|
| CHRISTOPHER RUPP，等: "Solubilization of poorly water-soluble drugs by mixed micelles based on hydrogenated phosphatidylcholine", 《INTERNATIONAL JOURNAL OF PHARMACEUTICS》 * |
| HANS LAUTENSCBLAGER: "在皮肤制剂中的脂质体（二）", 《日用化学工业译丛》 * |
| TOMOKO NII,等: "Encapsulation efficiency of water-soluble and insoluble drugs in liposomes prepared by the microencapsulation vesicle method", 《INTERNATIONAL JOURNAL OF PHARMACEUTICS》 * |
| 李丽: "大豆磷脂及其在药剂中的应用", 《黑龙江医药》 * |
| 王思玲，等: "《胶体分散药物制剂》", 31 December 2006, 人民卫生出版社 * |
| 陈军，等: "磷脂组成对马钱子总生物碱隐形脂质体抗肿瘤作用的影响", 《中成药》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105078776A (en) * | 2015-09-01 | 2015-11-25 | 赵洪波 | Cosmetic active matter carrier coating and preparation method thereof |
| CN111132753A (en) * | 2017-09-04 | 2020-05-08 | 一丸自然美建有限公司 | pH-sensitive liposome and method for producing same |
| CN111132753B (en) * | 2017-09-04 | 2022-05-27 | 一丸自然美健有限公司 | pH-sensitive liposome and method for producing same |
| CN111374947A (en) * | 2018-12-25 | 2020-07-07 | 太阳星光齿磨公司 | Liposome, liposome-containing composition, method for producing liposome, and method for improving thermal stability of retinol |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Zhang et al. | Essential oil-mediated glycerosomes increase transdermal paeoniflorin delivery: Optimization, characterization, and evaluation in vitro and in vivo | |
| Rehman et al. | Recent advances in gel technologies for topical and transdermal drug delivery | |
| Alexander et al. | Recent expansions in an emergent novel drug delivery technology: Emulgel | |
| CN102327199B (en) | Cosmetic composition comprising double-shell nano-structure | |
| CN102614091B (en) | Resveratrol nanostructured lipid carrier and preparation method thereof | |
| CN101658468B (en) | Coenzyme Q nanostructured lipid carrier and preparation method thereof | |
| KR20160093065A (en) | Lipid microcapsules preferably comprising a lipophilic active substance and composition containing same, method for the production thereof, and use of same in dermatology and in cosmetics | |
| CA2193359A1 (en) | Compositions for applying active substances to or through the skin | |
| CN102688153A (en) | Blank liposome prepared by compounding phospholipid and preparation method thereof | |
| CN105232342A (en) | Solid lipid carrier having multiple-structure and preparation method thereof | |
| Sandhu et al. | Surface-tailored nanomixed micelles containing quercetin–salicylic acid physical complex for enhanced cellular and in vivo activities: a quality by design perspective | |
| CN101396344A (en) | Paeonol microemulsion preparation and preparation method thereof | |
| EP2769709B1 (en) | Pseudolipid complex mixture and a skin external application composition containing same | |
| CN108926534A (en) | KGM modified lecithin carries the transdermal alcohol plastid of NMN, preparation and its preparation process and application | |
| DE4021083A1 (en) | Phospholipid formulations for medicinal and cosmetic baths - contg. phosphatidyl choline, oil component e.g. wax ester, alcohol, stabiliser, active ingredient and opt. auxiliaries | |
| US20100087394A1 (en) | Compositions Containing Betaine and Hydrophobic Silica | |
| CN102697664B (en) | Tanshinone lipidosome and preparation method thereof | |
| KR101761005B1 (en) | Cosmetic composition containing non-toxic sulfur for sikn moisture, anti-inflammatory and immune enhancement and manufacturing the same | |
| CN109316446A (en) | A kind of polyglutamic acid and hEGF's nano liposomes and preparation method thereof | |
| CN106580758A (en) | Oil-control hydraulic elusion and preparation method thereof | |
| TWI658840B (en) | Emulsified liposome composition and method for making the same | |
| CN111249174A (en) | Double-shell-core structure composition and preparation method thereof | |
| Christania et al. | Lipid and silver nanoparticles gels formulation of tempeh extract | |
| CN105520862A (en) | Sea buckthorn oil liposome and method for preparing same | |
| CN108969396A (en) | A kind of gel skin care item and preparation method thereof containing NMN |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20120926 |