CN102676402A - Semi-synthetic medium for Aschersonia placenta - Google Patents
Semi-synthetic medium for Aschersonia placenta Download PDFInfo
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- CN102676402A CN102676402A CN2012101662236A CN201210166223A CN102676402A CN 102676402 A CN102676402 A CN 102676402A CN 2012101662236 A CN2012101662236 A CN 2012101662236A CN 201210166223 A CN201210166223 A CN 201210166223A CN 102676402 A CN102676402 A CN 102676402A
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Abstract
The invention relates to a semi-synthetic medium, in particular to a semi-synthetic medium for Aschersonia placenta. The semi-synthetic medium for the Aschersonia placenta comprises 5.0-20.0 grams of carbon, 0.5-2.5 grams of nitrogen, 0.5-1.5 grams of potassium dihydrogen phosphate and 0.5-1.0 grams of magnesium sulfate, which are supplemented to 1 liter by water, wherein the carbon source is sucrose, soluble starch or mannitol, and the nitrogen source is yeast extract, pancreatic peptone or beef extract. The semi-synthetic medium for the Aschersonia placenta is clear in composition; when used for producing Aschersonia placenta mycelium, the semi-synthetic medium for the Aschersonia placenta has the advantages that the process is simple, heavy metal pollution is avoided, the Aschersonia placenta grows fast, the mycelium yield is high (as high as 28 g/L or above), and the product quality is stable; and the semi-synthetic medium for the Aschersonia placenta is applicable to both physiological and biochemical research on the Aschersonia placenta and industrial fermentation of the Aschersonia placenta.
Description
Technical field
The present invention relates to a kind of flat seat shell spore substratum, particularly a kind of flat seat shell spore semisynthetic medium.
Background technology
Flat seat shell spore [
Aschersonia placentaBerk.] be under the jurisdiction of mycota (Kingdom Fungi), Ascomycota (Ascomycota), caprophyl guiding principle (
Sordariomycetes), Hypocreales (
Hypocreales), Clavicipitaceae (
Clavicipitaceae), a seat shell spore belong to (
Aschersonia), be aleyrodid (
Aleyrodidae) and scale insect (
Coccidae) important pathogenic fungi, be distributed widely in the torrid zone and subtropical zone.Early stage research both at home and abroad shows that flat seat shell spore can effectively be controlled aleyrodid and these two types of suckings pest of scale insect, at aspects such as biological control of insect pests, environment-protection pesticide exploitations application promise in clinical practice is arranged.But; Domestic and international in recent years many units have carried out the Study on artificial culture of flat seat shell spore bacterium; The entering that has suitability for industrialized production, but fermention medium all adopts natural medium (as: with animal, plant, mikrobe or the formulated substratum of other natural and organic ingredients).Because the natural medium complicated component, each batch material nutrient component content is unstable, and fermenting process and quality product are wayward.In addition, each nutritive ingredient of natural medium is indeterminate, is inappropriate for the research of flat seat shell spore bacterium Physiology and biochemistry.
Summary of the invention
In order to address the above problem, the invention provides a kind of flat seat shell spore bacterium semisynthetic medium, its moity is clear and definite, and leavened prod is quality controllable, is suitable for this commerial scale fermentative prodn.
A kind of flat seat shell spore bacterium semisynthetic medium of the present invention; Form by carbon source, nitrogenous source, potassium primary phosphate, sal epsom and water; Wherein carbon (pressing the carbon element content that molecular weight calculates) 5.0～20.0 restrains, nitrogen (nitrogen element content) 0.5～2.5 gram, potassium primary phosphate 0.5～1.5 gram; Sal epsom 0.5～1.0 gram, water is settled to 1 liter; Said carbon source is sucrose, Zulkovsky starch or N.F,USP MANNITOL, and nitrogenous source is that yeast soaks powder, Tryptones or beef extract.In said flat seat shell spore bacterium semisynthetic medium, add the quality percentage composition and be 1.5～2.0% agar and obtain solid medium.
Preferably, said flat seat shell spore bacterium semisynthetic medium is: sucrose 25.0～45.0 grams, yeast soak powder 5.0～10.0 grams, potassium primary phosphate 0.5～1.5 gram, and sal epsom 0.5～1.0 gram, water is settled to 1 liter.In said flat seat shell spore bacterium semisynthetic medium, add the quality percentage composition and be 1.5～2.0% agar and obtain solid medium.
More preferably, said flat seat shell spore bacterium semisynthetic medium is: sucrose 45.0 grams, yeast soak powder 10.0 grams, potassium primary phosphate 0.5 gram, and sal epsom 0.5 gram, water is settled to 1 liter.In said flat seat shell spore bacterium semisynthetic medium, add the quality percentage composition and be 1.5～2.0% agar and obtain solid medium.
Above-mentioned flat seat shell spore bacterium semisynthetic medium all can be prepared by ordinary method, as weighs above-mentioned quality proportion raw material and put into container, and heating for dissolving mixes, and sterilization gets final product.
Flat seat shell spore bacterium semisynthetic medium of the present invention, its moity is clear and definite, the raw material homogeneous of fermentation; The leavened prod quality can be controlled, and utilizes the flat seat of this substratum fermentative prodn shell spore to have technology and simply, does not have heavy metal contamination, and thalli growth speed is fast; Mycelia productive rate high (can reach more than 28 g/L); Advantages such as constant product quality both had been suitable for the research of flat seat shell spore bacterium physio-biochemical characteristics, also were suitable for this commerial scale fermentative prodn.
Embodiment
Embodiment 1, utilize flat seat shell spore bacterium semisynthetic medium to produce flat seat shell spore mycelia
Will be from separation and purification on the aleyrodid worm corpse of the flat seat of natural infection shell spore bacterium; And the inoculation that is accredited as real flat seat shell spore by morphological specificity and molecular biology evidence is to flat seat shell spore bacterium semisynthetic medium and control medium, respectively according to ordinary method fermentative prodn mycelia.The result shows that the mycelia productive rate of flat seat shell spore bacterium semisynthetic medium reaches (23 g/L) 28 g/L, and the mycelia productive rate of control medium is merely 5.1 g/L.Wherein, the prescription of flat seat shell spore bacterium semisynthetic medium is sucrose (35.0 gram) 45.0 grams, and yeast soaks powder (7.5 gram) 10.0 grams, potassium primary phosphate 0.5 gram, sal epsom 0.5 gram, 1 liter of zero(ppm) water.The mycelia productive rate that the expression different ingredients obtains in the above-mentioned bracket.The contrast culture based formulas is 1 liter of ammonium sulfate 2g, Repone K 0.5g, sal epsom 0.5g, potassium primary phosphate 0.5g, Sodium phosphate, dibasic 0.65g, zero(ppm) water.
Embodiment 2, utilize flat seat shell spore bacterium semisynthetic medium to produce flat seat shell spore mycelia
Will be from separation and purification on the aleyrodid worm corpse of the flat seat of natural infection shell spore bacterium; And the inoculation that is accredited as real flat seat shell spore by morphological specificity and molecular biology evidence is to flat seat shell spore bacterium semisynthetic medium and control medium, respectively according to ordinary method fermentative prodn mycelia.The result shows that the mycelia productive rate of flat seat shell spore bacterium semisynthetic medium reaches (22 g/L) 26 g/L, and the mycelia productive rate of control medium is merely 5.1 g/L.Wherein, the prescription of flat seat shell spore bacterium semisynthetic medium is N.F,USP MANNITOL (35.0 gram) 45.0 grams, and yeast soaks powder (7.5 gram) 10.0 grams, potassium primary phosphate 0.5 gram, sal epsom 0.5 gram, 1 liter of zero(ppm) water.The contrast culture based formulas is 1 liter of ammonium sulfate 2g, Repone K 0.5g, sal epsom 0.5g, potassium primary phosphate 0.5g, Sodium phosphate, dibasic 0.65g, zero(ppm) water.
Embodiment 3, utilize flat seat shell spore bacterium semisynthetic medium to produce flat seat shell spore mycelia
Will be from separation and purification on the aleyrodid worm corpse of the flat seat of natural infection shell spore bacterium; And the inoculation that is accredited as real flat seat shell spore by morphological specificity and molecular biology evidence is to flat seat shell spore bacterium semisynthetic medium and control medium, respectively according to ordinary method fermentative prodn mycelia.The result shows that the mycelia productive rate of flat seat shell spore bacterium semisynthetic medium reaches (12 g/L) 15 g/L, and the mycelia productive rate of control medium is merely 5.1 g/L.Wherein, the prescription of flat seat shell spore bacterium semisynthetic medium is N.F,USP MANNITOL (35.0 gram) 45.0 grams, beef extract (7.5 gram) 10.0 grams, potassium primary phosphate 0.5 gram, sal epsom 0.5 gram, 1 liter of zero(ppm) water.The contrast culture based formulas is 1 liter of ammonium sulfate 2g, Repone K 0.5g, sal epsom 0.5g, potassium primary phosphate 0.5g, Sodium phosphate, dibasic 0.65g, zero(ppm) water.
Embodiment 4, utilize flat seat shell spore bacterium semisynthetic medium to produce flat seat shell spore mycelia
Will be from separation and purification on the aleyrodid worm corpse of the flat seat of natural infection shell spore bacterium; And the inoculation that is accredited as real flat seat shell spore by morphological specificity and molecular biology evidence is to flat seat shell spore bacterium semisynthetic medium and control medium, respectively according to ordinary method fermentative prodn mycelia.The result shows that the mycelia productive rate of flat seat shell spore bacterium semisynthetic medium reaches (20 g/L) 24 g/L, and the mycelia productive rate of control medium is merely 5.1 g/L.Wherein, the prescription of flat seat shell spore bacterium semisynthetic medium is sucrose (35.0 gram) 45.0 grams, Tryptones (7.5 gram) 10.0 grams, potassium primary phosphate 0.5 gram, sal epsom 0.5 gram, 1 liter of zero(ppm) water.The contrast culture based formulas is 1 liter of ammonium sulfate 2g, Repone K 0.5g, sal epsom 0.5g, potassium primary phosphate 0.5g, Sodium phosphate, dibasic 0.65g, zero(ppm) water.
Embodiment 5, utilize flat seat shell spore bacterium semisynthetic medium to produce flat seat shell spore mycelia
Will be from separation and purification on the aleyrodid worm corpse of the flat seat of natural infection shell spore bacterium; And the inoculation that is accredited as real flat seat shell spore by morphological specificity and molecular biology evidence is to flat seat shell spore bacterium semisynthetic medium and control medium, respectively according to ordinary method fermentative prodn mycelia.The result shows that the mycelia productive rate of flat seat shell spore bacterium semisynthetic medium reaches (15 g/L) 18 g/L, and the mycelia productive rate of control medium is merely 5.1 g/L.Wherein, the prescription of flat seat shell spore bacterium semisynthetic medium is sucrose (35.0 gram) 45.0 grams, beef extract (7.5 gram) 10.0 grams, potassium primary phosphate 0.5 gram, sal epsom 0.5 gram, 1 liter of zero(ppm) water.The contrast culture based formulas is 1 liter of ammonium sulfate 2g, Repone K 0.5g, sal epsom 0.5g, potassium primary phosphate 0.5g, Sodium phosphate, dibasic 0.65g, zero(ppm) water.
Embodiment 6, utilize flat seat shell spore bacterium semisynthetic medium to produce flat seat shell spore mycelia
Will be from separation and purification on the aleyrodid worm corpse of the flat seat of natural infection shell spore bacterium; And the inoculation that is accredited as real flat seat shell spore by morphological specificity and molecular biology evidence is to flat seat shell spore bacterium semisynthetic medium and control medium, respectively according to ordinary method fermentative prodn mycelia.The result shows that the mycelia productive rate of flat seat shell spore bacterium semisynthetic medium reaches (19 g/L) 23 g/L, and the mycelia productive rate of control medium is merely 5.1 g/L.Wherein, the prescription of flat seat shell spore bacterium semisynthetic medium is soluble starch (35.0 gram) 45.0 grams, and yeast soaks powder (7.5 gram) 10.0 grams, potassium primary phosphate 0.5 gram, sal epsom 0.5 gram, 1 liter of zero(ppm) water.The contrast culture based formulas is 1 liter of ammonium sulfate 2g, Repone K 0.5g, sal epsom 0.5g, potassium primary phosphate 0.5g, Sodium phosphate, dibasic 0.65g, zero(ppm) water.
Embodiment 7, utilize flat seat shell spore bacterium semisynthetic medium to produce flat seat shell spore mycelia
Will be from separation and purification on the aleyrodid worm corpse of the flat seat of natural infection shell spore bacterium; And the inoculation that is accredited as real flat seat shell spore by morphological specificity and molecular biology evidence is to flat seat shell spore bacterium semisynthetic medium and control medium, respectively according to ordinary method fermentative prodn mycelia.The result shows that the mycelia productive rate of flat seat shell spore bacterium semisynthetic medium reaches (12 g/L) 17 g/L, and the mycelia productive rate of control medium is merely 5.1 g/L.Wherein, the prescription of flat seat shell spore bacterium semisynthetic medium is soluble starch (35.0 gram) 45.0 grams, Tryptones (7.5 gram) 10.0 grams, potassium primary phosphate 0.5 gram, sal epsom 0.5 gram, 1 liter of zero(ppm) water.The contrast culture based formulas is 1 liter of ammonium sulfate 2g, Repone K 0.5g, sal epsom 0.5g, potassium primary phosphate 0.5g, Sodium phosphate, dibasic 0.65g, zero(ppm) water.
Embodiment 8, utilize flat seat shell spore bacterium semisynthetic medium to produce flat seat shell spore mycelia
Will be from separation and purification on the aleyrodid worm corpse of the flat seat of natural infection shell spore bacterium; And the inoculation that is accredited as real flat seat shell spore by morphological specificity and molecular biology evidence is to flat seat shell spore bacterium semisynthetic medium and control medium, respectively according to ordinary method fermentative prodn mycelia.The result shows that the mycelia productive rate of flat seat shell spore bacterium semisynthetic medium reaches (9 g/L) 12 g/L, and the mycelia productive rate of control medium is merely 5.1 g/L.Wherein, the prescription of flat seat shell spore bacterium semisynthetic medium is soluble starch (35.0 gram) 45.0 grams, beef extract (7.5 gram) 10.0 grams, potassium primary phosphate 0.5 gram, sal epsom 0.5 gram, 1 liter of zero(ppm) water.The contrast culture based formulas is 1 liter of ammonium sulfate 2g, Repone K 0.5g, sal epsom 0.5g, potassium primary phosphate 0.5g, Sodium phosphate, dibasic 0.65g, zero(ppm) water.
Embodiment 9, utilize flat seat shell spore bacterium semisynthetic medium to produce flat seat shell spore mycelia
Will be from separation and purification on the aleyrodid worm corpse of the flat seat of natural infection shell spore bacterium; And the inoculation that is accredited as real flat seat shell spore by morphological specificity and molecular biology evidence is to flat seat shell spore bacterium semisynthetic medium and control medium, respectively according to ordinary method fermentative prodn mycelia.The result shows that the mycelia productive rate of flat seat shell spore bacterium semisynthetic medium reaches (16 g/L) 20 g/L, and the mycelia productive rate of control medium is merely 5.1 g/L.Wherein, the prescription of flat seat shell spore bacterium semisynthetic medium is N.F,USP MANNITOL (35.0 gram) 45.0 grams, Tryptones (7.5 gram) 10.0 grams, potassium primary phosphate 0.5 gram, sal epsom 0.5 gram, 1 liter of zero(ppm) water.The contrast culture based formulas is 1 liter of ammonium sulfate 2g, Repone K 0.5g, sal epsom 0.5g, potassium primary phosphate 0.5g, Sodium phosphate, dibasic 0.65g, zero(ppm) water.
Claims (6)
1. a flat seat shell spore bacterium semisynthetic medium is made up of carbon source, nitrogenous source, potassium primary phosphate, sal epsom and water, and wherein carbon 5.0～20.0 restrains, nitrogen 0.5～2.5 gram, and potassium primary phosphate 0.5～1.5 gram, sal epsom 0.5～1.0 gram, water is settled to 1 liter; Said carbon source is sucrose, Zulkovsky starch or N.F,USP MANNITOL, and nitrogenous source is that yeast soaks powder, Tryptones or beef extract.
2. flat seat shell spore bacterium semisynthetic medium according to claim 1 is characterized in that: in said flat seat shell spore bacterium semisynthetic medium, add the quality percentage composition and be 1.5～2.0% agar and obtain solid medium.
3. flat seat shell spore bacterium semisynthetic medium according to claim 1; It is characterized in that: said flat seat shell spore bacterium semisynthetic medium is: sucrose 25.0～45.0 grams, yeast soak powder 5.0～10.0 grams, potassium primary phosphate 0.5～1.5 gram; Sal epsom 0.5～1.0 gram, water is settled to 1 liter.
4. flat seat shell spore bacterium semisynthetic medium according to claim 3 is characterized in that: in said flat seat shell spore bacterium semisynthetic medium, add the quality percentage composition and be 1.5～2.0% agar and obtain solid medium.
5. flat seat shell spore bacterium semisynthetic medium according to claim 3 is characterized in that: said flat seat shell spore bacterium semisynthetic medium is: sucrose 45.0 grams, yeast soak powder 10.0 grams, potassium primary phosphate 0.5 gram, and sal epsom 0.5 gram, water is settled to 1 liter.
6. flat seat shell spore bacterium semisynthetic medium according to claim 5 is characterized in that: in said flat seat shell spore bacterium semisynthetic medium, add the quality percentage composition and be 1.5～2.0% agar and obtain solid medium.
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| CN2012101662236A CN102676402A (en) | 2012-05-26 | 2012-05-26 | Semi-synthetic medium for Aschersonia placenta |
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| CN2012101662236A CN102676402A (en) | 2012-05-26 | 2012-05-26 | Semi-synthetic medium for Aschersonia placenta |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103275950A (en) * | 2013-06-09 | 2013-09-04 | 福建农林大学 | Culture medium and method for producing lipase by aschersonia placenta fermentation |
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2012
- 2012-05-26 CN CN2012101662236A patent/CN102676402A/en active Pending
Non-Patent Citations (3)
| Title |
|---|
| 王震: "扁座壳孢菌生理特性及乳悬剂的初步研究", 《中国优秀硕士学位论文全文数据库》 * |
| 邱君志: "不同pH值对粉虱座壳孢菌孢子萌发和菌丝生长的影响", 《莱阳农学院学报》 * |
| 邱君志: "粉虱座壳孢菌培养基的筛选试验", 《河南科技大学学报：自然科学版》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103275950A (en) * | 2013-06-09 | 2013-09-04 | 福建农林大学 | Culture medium and method for producing lipase by aschersonia placenta fermentation |
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Application publication date: 20120919 |