CN102675442A - Method for extracting leaf protein and albumin from mustard leaf - Google Patents
Method for extracting leaf protein and albumin from mustard leaf Download PDFInfo
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- CN102675442A CN102675442A CN2012101986409A CN201210198640A CN102675442A CN 102675442 A CN102675442 A CN 102675442A CN 2012101986409 A CN2012101986409 A CN 2012101986409A CN 201210198640 A CN201210198640 A CN 201210198640A CN 102675442 A CN102675442 A CN 102675442A
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Abstract
The invention discloses a method for extracting a leaf protein and an albumin from a mustard leaf. The method particularly comprises the following steps of: 1, washing the mustard leaf, and then, mincing, adding water to pulp, squeezing to obtain leaf juice, loading in a container, heating, preserving heat for a certain time, and then, cooling, vibrating the leaf juice, precipitating the leaf protein, and afterwards, pouring out upper liquid juice for later use, wherein a substance at the bottom is the leaf protein; subsequently, adding water into the leaf protein for many times, agitating, vibrating, and then, removing a floater and precipitating to remove nitrate, and quickly drying the leaf protein with higher purity by adopting a high temperature to remove glucosinolate and a degradation product of the glucosinolate; and 2, secondarily heating the liquid juice which is poured out for the later use to a certain temperature, preserving heat for a certain time, precipitating the albumin, subsequently, removing upper liquid, adding water, and then, removing the nitrate, the glucosinolate and the degradation product of the glucosinolate by using a processing method the same as that of the leaf protein. The process is simple and feasible and is convenient to operate; used equipment is simple; the practicability is strong; the cost performance of an obtained product is high; and the resource utilization rate is effectively improved.
Description
Technical field
The present invention relates to a kind of leaf protein and albuminous method from mustard leaf, extracted.
Background technology
The main raw material crop of hot pickled mustard tube processing in the course of processing, has more than 1,200,000 tons mustard leaf fail to make full use of every year.Owing to contain anti-nutrient molecules such as nitrate salt, glucosinolate and S-methylcysteine sulfoxide in the mustard leaf,, cause easily that also livestock poisons as the feed bitter.Because developing country all is faced with the protein crisis, and China has a large population and a few land, protein provision is very in short supply, and only feedstuff protein just lacks 1 * 10 every year
8T is seriously restricting the development of fodder industry and aquaculture, and therefore, the protein resource that active development is new has great importance.Leaf protein for mustard leaf utilizes fully, will be a kind of serious waste to resource, and its contained nitrate salt can make also that soil receives serious pollution, existent environment of people is produced greatly threaten.Therefore, adopt reasonable and economic method development and use mustard leaf to seem very important.
Summary of the invention
The purpose of this invention is to provide a kind of mustard leaf that utilizes and extract leaf protein and BSA, and can effectively remove the method for the processing mustard leaf of ANFs such as nitrate salt in leaf protein and the BSA, glucosinolate.
For realizing above-mentioned purpose, described mustard leaf extraction leaf protein of the present invention and albuminous method are following.
One, with mustard leaf through cleaning the back chopping, add the water making beating again, afterwards squeeze juice again; With the leaf juice that squeezes put into heat behind the container and be incubated certain hour after cool off, to be cooled behind normal temperature, vibrate leaf juice again; Make the leaf protein deposition, treat the leaf protein post precipitation, it is for use to pour out top juice; The material of bottom is leaf protein, afterwards repeatedly with clear water add in the leaf protein stir and vibration after float clearly again, leaf protein precipitated once more and remove denitrification; Obtain the higher leaf protein of purity, adopt fast that purity is the higher leaf protein oven dry of high temperature again and removal glucosinolate and degraded product thereof.
Two, the top juice that will pour out again carries out second-heating to certain temperature and is incubated the regular hour, makes the BSA deposition, and liquid that afterwards will be top is removed, add clear water stir and vibrate after float clearly again, precipitate and remove nitrate salt; Remove nitrate salt with floating clearly again, precipitate after BSA repeated multiple times adding clear water stirring and the vibration, extract highly purified BSA, adopt fast that purity is the higher BSA oven dry of high temperature again and removal glucosinolate and degraded product thereof.
In order to obtain higher leaf protein of purity and BSA, described mustard leaf of the present invention extract in leaf protein and the albuminous method leaf protein and BSA adopt repeatedly add clear water stir and vibrate after float clearly again, precipitate and remove nitrate salt.
In order to obtain leaf protein, described mustard leaf of the present invention extracts and in leaf protein and the albuminous method leaf juice of the mustard leaf that squeezes put into the temperature that heats behind the container is 60 ℃ to 97 ℃.
In order to obtain BSA, described mustard leaf of the present invention extract in leaf protein and the albuminous method will to extract the temperature of carrying out second-heating behind the leaf juice threading container behind the leaf protein be 70 ℃ to 98 ℃.
In order thoroughly to remove glucosinolate and the degraded product thereof in leaf protein and the BSA; Described mustard leaf of the present invention extracts in leaf protein and the albuminous method, adopts fast that purity is higher leaf protein of high temperature and BSA to dry and thoroughly removes glucosinolate and degraded product thereof; Described high temperature is to be higher than 200 ℃.
Mustard leaf of the present invention extracts leaf protein and albuminous method, and simple for process, easy to operate, used equipment is simple, and is practical, and the cost performance of product that obtains is high; Whole manufacturing process is pollution-free, effectively raises the utilization ratio of resource, has well solved the pollution problem of mustard leaf to environment, helps environment protection.
Description of drawings
Fig. 1 is the process flow diagram that mustard leaf of the present invention extracts leaf protein and albuminous method.
Embodiment
Be the preferred implementation that mustard leaf of the present invention extracts leaf protein and BSA method below.
One, with mustard leaf through cleaning up back chopping, add the water making beating again, afterwards squeeze juice again; The leaf juice that squeezes put into be heated to 85 ℃ to 90 ℃ and be incubated 5 to 10 minutes behind the container, cool off afterwards, to be cooled behind normal temperature; Vibrate leaf juice again, make the leaf protein deposition, treat the leaf protein post precipitation; It is for use to pour out top juice, and the material of bottom is leaf protein, afterwards repeatedly with clear water add in the leaf protein stir and vibration after float clearly again; Leaf protein is precipitated once more and remove denitrification, obtain the higher leaf protein of purity, adopt 230 ℃ to 280 ℃ fast that purity is the higher leaf proteins oven dry of high temperature again and remove glucosinolate and degraded product thereof.
Two, the top juice that will pour out again carries out second-heating to 90 ℃ to 95 ℃ and be incubated 5 to 10 minutes, makes the BSA deposition, and liquid that afterwards will be top is removed, add clear water stir and vibrate after float clearly again, precipitate and remove nitrate salt; Remove nitrate salt with floating clearly again, precipitate after BSA repeated multiple times adding clear water stirring and the vibration; Extract highly purified BSA, adopt 230 ℃ to 280 ℃ fast that purity is the higher BSA oven dry of high temperature again and removal glucosinolate and degraded product thereof.
Claims (5)
1. a mustard leaf extracts leaf protein and albuminous method, it is characterized in that: mustard leaf extract leaf protein and albuminous method following, one, with mustard leaf through cleaning the back chopping; Add water making beating again, squeeze juice more afterwards, with the leaf juice that squeezes put into heat behind the container and be incubated certain hour after cool off; To be cooled behind normal temperature, vibrate leaf juice again, make the leaf protein deposition; Treat the leaf protein post precipitation, it is for use to pour out top juice, and the material of bottom is leaf protein; Afterwards repeatedly with clear water add in the leaf protein stir and vibration after float clearly again; Leaf protein is precipitated once more and remove denitrification, obtain the higher leaf protein of purity, adopt fast that purity is the higher leaf protein oven dry of high temperature again and remove glucosinolate and degraded product thereof; Two, the top juice that will pour out again carries out second-heating to certain temperature and is incubated the regular hour, makes the BSA deposition, and liquid that afterwards will be top is removed, add clear water stir and vibrate after float clearly again, precipitate and remove nitrate salt; Remove nitrate salt with floating clearly again, precipitate after BSA repeated multiple times adding clear water stirring and the vibration, extract highly purified BSA, adopt fast that purity is the higher BSA oven dry of high temperature again and removal glucosinolate and degraded product thereof.
2. extract leaf protein and albuminous method based on the described mustard leaf of claim 1, it is characterized in that: mustard leaf extract leaf protein and albumin in leaf protein and the albuminous method adopt repeatedly add clear water stir and vibrate after float clearly again, precipitate and remove nitrate.
3. mustard leaf according to claim 1 extracts leaf protein and albuminous method, it is characterized in that: it is 70 ℃ to 97 ℃ that mustard leaf extracts the temperature that heats behind the leaf juice threading container with the mustard leaf that squeezes in leaf protein and the albuminous method.
4. mustard leaf according to claim 1 extracts leaf protein and albuminous method, it is characterized in that: mustard leaf extract in leaf protein and the albuminous method will to extract the temperature of carrying out second-heating behind the leaf juice threading container behind the leaf protein be 70 ℃ to 98 ℃.
5. mustard leaf according to claim 1 extracts leaf protein and albuminous method; It is characterized in that: mustard leaf extracts in leaf protein and the albuminous method, adopts fast that purity is higher leaf protein of high temperature and BSA to dry and thoroughly removes glucosinolate and degraded product thereof; Described high temperature is to be higher than 200 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101986409A CN102675442A (en) | 2012-06-17 | 2012-06-17 | Method for extracting leaf protein and albumin from mustard leaf |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101986409A CN102675442A (en) | 2012-06-17 | 2012-06-17 | Method for extracting leaf protein and albumin from mustard leaf |
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| CN102675442A true CN102675442A (en) | 2012-09-19 |
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| CN2012101986409A Pending CN102675442A (en) | 2012-06-17 | 2012-06-17 | Method for extracting leaf protein and albumin from mustard leaf |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1349755A (en) * | 2001-11-23 | 2002-05-22 | 西南农业大学 | Edible protein producing process with plant leaf |
| CN1943391A (en) * | 2006-11-09 | 2007-04-11 | 武汉光盛生物工程技术开发有限公司 | Composite extracting method for plant leaf protein and its use on hybrid giant napier |
| CN101371678A (en) * | 2008-09-04 | 2009-02-25 | 甘肃省农业科学院畜草品种改良研究所 | Method for directly separating and extracting leaf protein of heart trefoil for feeding and eating |
-
2012
- 2012-06-17 CN CN2012101986409A patent/CN102675442A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1349755A (en) * | 2001-11-23 | 2002-05-22 | 西南农业大学 | Edible protein producing process with plant leaf |
| CN1943391A (en) * | 2006-11-09 | 2007-04-11 | 武汉光盛生物工程技术开发有限公司 | Composite extracting method for plant leaf protein and its use on hybrid giant napier |
| CN101371678A (en) * | 2008-09-04 | 2009-02-25 | 甘肃省农业科学院畜草品种改良研究所 | Method for directly separating and extracting leaf protein of heart trefoil for feeding and eating |
Non-Patent Citations (2)
| Title |
|---|
| 邓勇 等: "叶蛋白的提取工艺", 《中国农业大学学报》 * |
| 郑力: "苜蓿叶蛋白的提取方法及展望", 《饲料工业》 * |
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Application publication date: 20120919 |