CN102671059A - Smilax extract, and preparation method and application thereof - Google Patents
Smilax extract, and preparation method and application thereof Download PDFInfo
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- CN102671059A CN102671059A CN2011100653790A CN201110065379A CN102671059A CN 102671059 A CN102671059 A CN 102671059A CN 2011100653790 A CN2011100653790 A CN 2011100653790A CN 201110065379 A CN201110065379 A CN 201110065379A CN 102671059 A CN102671059 A CN 102671059A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention provides a preparation method of a smilax rhizome extract, the smilax rhizome extract prepared by the method and application of the smilax rhizome extract to preparation of medicines for preventing, relieving and treating inflammation. The content of total saponin ingredients in the smilax rhizome extract is high, the total saponin ingredients account for over 50% of the weight of the smilax rhizome extract, pharmacodynamics tests prove that the therapeutic effect of the total saponin ingredients is remarkable, the total saponin ingredients are a principal medical effect material basis for treating gynecological inflammation through smilax, and can serve as a raw material of new drugs for effective part of traditional Chinese medicines.
Description
Technical field
The invention belongs to field of medicaments, relating to specifically with plant amedica Rhizoma Smilacis Chinensis rhizome is that raw material extracts the activity extract that contains the total saponins compounds.The invention still further relates to the method for preparing and the purposes of this activity extract.
Background technology
World Health Organization's latest survey report is pointed out; Modal disease is not to catch a cold but gynaecopathia (comprise cervical disease, gynecological tumor, gynecological inflammation and stop various infection and the complication that unexpected gestation is caused) in the women, and wherein gynecological inflammation accounts for 80% of gynaecopathia.The women at reproduction age of China about 41% suffers from gynecological inflammation in various degree, and married women's sickness rate is more up to 70%, and this type of disease has had a strong impact on women's orthobiosis, work and physical and mental health.
Western medical treatment gynecopathy such as pelvic inflammatory diseases associated with inflammation mainly adopts antibiotic; Clinical research shows; Antibiosis have the effect of the control acute pelvic inflammatory disease disease state of an illness, but can't effect a radical cure, and antibiotic is not obvious to the chronic pelvic inflammation curative effect simultaneously; Use complication or untoward reaction such as being prone to cause alteration of intestinal flora repeatedly, and side effect is more.Chinese medicine is according to etiology and pathogenesis, and from the integral body sight, dialectical opinion is controlled, and has brought into play certain advantage, no matter is in use medical time-honored China in recent years, has still all become the research focus in Japan, Korea S.
Rhizoma Smilacis Chinensis, the beginning is stated from " Mingyi Bielu ", is newly-increased kinds of 2005 editions Chinese Pharmacopoeias; Its medicinal part is the dry rhizome of liliaceous plant Rhizoma Smilacis Chinensis Smilax china L., has expelling wind and removing dampness, the effect of detoxifcation dissipating blood stasis; Be mainly used in muscles and bones aching; dribbling urination, profuse leukorrhea, the treatment of the too fat to move grade of furuncle.Rhizoma Smilacis Chinensis just had the record as medicine in China before 400 years, since nineteen sixties, its total extract preparation has been widely used in female chronic inflammation, adnexitis and has regulated diseases such as immunity, blood circulation promoting and blood stasis dispelling, antitumor.
Existing Rhizoma Smilacis Chinensis preparation all is to be got through the water decoction-alcohol sedimentation prepared by the Rhizoma Smilacis Chinensis medical material, has the shortcoming that the active component extraction ratio is low, dose is big, thereby has limited the clinical practice and the market scale of this medicine.
Summary of the invention
The objective of the invention is to develop determined curative effect, the clear and definite Rhizoma Smilacis Chinensis rhizome effective parts formulation of active component.
In one aspect of the invention, the method for preparing of Radix Smilacis Chinae stem extract is provided, it may further comprise the steps:
(1) with solvent extraction Rhizoma Smilacis Chinensis rhizome medical material;
(2) extracting solution is concentrated, be dissolved in water again;
(3) water-soluble liquid that step (2) is obtained passes through macroporous adsorbent resin, the methanol of water or 1～20 weight % or ethanol water drip washing;
(4) with methanol or the ethanol water eluting of 30～95 weight %, collect eluent, remove and desolvate, be drying to obtain Rhizoma Smilacis Chinensis extract.
One of the present invention preferred aspect, the solvent in the said step (1) is selected from one or more in water, methanol, ethanol and the acetone, is preferably water and/or ethanol.
One of the present invention preferred aspect, the method for extracting in the said step (1) is warm macerating, hot reflux and/or percolation.
Spissated method is preferably concentrate under reduced pressure at low temperature in the said step (2).
One of the present invention preferred aspect, macroporous adsorbent resin is selected from one or more in nonpolar macroporous adsorption resin, low pole macroporous adsorbent resin, Semi-polarity macroporous adsorbent resin, the polar macroporous adsorption resin described in the step (3); Preferably, said macroporous adsorbent resin is selected from one or more in HPD-300 macroporous adsorbent resin, HPD-100 macroporous adsorbent resin, D101 macroporous adsorbent resin, X-5 macroporous adsorbent resin, H103 macroporous adsorbent resin, H107 macroporous adsorbent resin, AB-8 macroporous adsorbent resin, DA-201 macroporous adsorbent resin, HPD-400 macroporous adsorbent resin, ML-8 macroporous adsorbent resin, NKA-9 macroporous adsorbent resin, NKA-II macroporous adsorbent resin, S-8 macroporous adsorbent resin, HPD-500 macroporous adsorbent resin, the HPD-600 macroporous adsorbent resin.
One of the present invention preferred aspect, the methanol in the step (3) or the volume fraction of ethanol water can be 1%～20%; Preferably, the volume fraction of said solution is 15%-20%.
One of the present invention preferred aspect, the methanol in the step (4) or the volume fraction of ethanol water can be 30%～95%, preferably, the volume fraction of said solution is 70%-80%.
Aspect second of the present invention, the Radix Smilacis Chinae that makes through said method stem extract is provided, wherein comprise the total saponins compounds.
Said Radix Smilacis Chinae stem extract mainly is made up of the saponins compound in the Rhizoma Smilacis Chinensis.Preferably, saponins compound accounts for more than 50% of Radix Smilacis Chinae stem extract weight.Said saponins compound mainly comprises the steroidal saponin that is made up of multiple diosgenin, Sarsasapogenin.
Inventor of the present invention finds that the saponin component that is comprised in this plant has the effect of obvious treatment gynecological inflammation property disease on the basis that Rhizoma Smilacis Chinensis rhizome effective site is studied.
Therefore, aspect the 3rd of the present invention, provide above-mentioned Radix Smilacis Chinae stem extract to be used for preventing, alleviating or treat the application of the medicine of inflammation in preparation; Preferably, said inflammation is caused by staphylococcus aureus and escherichia coli; Preferably, said inflammation is a gynecological inflammation; Again preferably, said gynecological inflammation is selected from acute pelvic inflammatory disease, chronic pelvic inflammatory disease, vaginitis, cervicitis, adnexitis, inflammatory masses of ovarian appendages.
One of the present invention preferred aspect, said medicine is capsule, tablet, granule, drop pill, soft capsule, dispersible tablet, oral cavity disintegration tablet, slow releasing tablet, controlled release tablet, injection, transfusion or the injectable sterile powder that comprises the Radix Smilacis Chinae stem extract.
Can adopt conventional method with Radix Smilacis Chinae stem extract and any or multiple pharmaceutic adjuvant, be mixed and made into above-mentioned dosage form like starch, dextrin, lactose, microcrystalline Cellulose, hydroxypropyl methylcellulose, low-substituted hydroxypropyl cellulose, polyvinylpolypyrrolidone, carboxymethyl starch sodium, Polyethylene Glycol, magnesium stearate, micropowder silica gel, glucose, mannitol, xylitol, glycine etc.
Pharmacodynamics to medicine of the present invention has partly carried out preliminary study, and the result finds that medicine of the present invention has significant antiinflammatory and bacteriostasis.
Compared with prior art, the present invention has following beneficial effect:
Method for preparing provided by the invention is simple, extraction effect good, be easy to industry applies.
The content of total saponins constituents is high in the Radix Smilacis Chinae stem extract that the present invention obtains; Its weight accounts for more than 50% of Radix Smilacis Chinae stem extract weight; Prove that through pharmacodynamics test it is evident in efficacy; Be the main pharmacodynamics material base of Rhizoma Smilacis Chinensis treatment gynecological inflammation, can be used as the raw material of effective ingredient in Chinese new drug.
Crude drug of the present invention source is abundant, and preparation technology is easy to industrialization, can process various dosage forms as required, for clinical convenient, effective and quality controllable modern Chinese medicine is provided.
The specific embodiment
Below in conjunction with the specific embodiment the present invention is further described in detail, the embodiment that provides has been merely and has illustrated the present invention, rather than in order to limit scope of the present invention.
Embodiment 1
Present embodiment is used to explain the method for preparing of Radix Smilacis Chinae stem extract of the present invention.
(1) gets Rhizoma Smilacis Chinensis rhizome 5.0kg (available from Anguo medical material wholesale market, Henan, Hubei, the place of production),, collect extracting solution with the alcohol heating reflux extraction 3 times (extracted respectively 2 hours, 1 hour and 1 hour, each alcohol solvent consumption is 50L) of 60 volume %;
(2) the extracting solution concentrate under reduced pressure at low temperature is not distinguished the flavor of to there being alcohol, add an amount of water and dissolve.
(3) water-soluble liquid that step (2) is obtained is crossed HPD-300 type macroporous adsorbent resin, and is extremely colourless with 3BV (BV:Bed Volume abbreviation, resin bed volume) water and 3BV 20 volume % ethanol drip washing earlier, discards leacheate;
(4) ethanol elution of reuse 5BV 70 volume % is collected ethanol elution, with the eluent drying under reduced pressure behind the recovery ethanol, gets Radix Smilacis Chinae stem extract (Rhizoma Smilacis Chinensis rhizome effective site) 185 grams.
The preparation of embodiment 2 Radix Smilacis Chinae stem extracts
Get Rhizoma Smilacis Chinensis rhizome 5.0kg (available from Anguo medical material wholesale market, Henan, Hubei, the place of production),, collect extracting solution with 70% ethanol (volume) heating and refluxing extraction 3 times (2h, 1h, 1h, each alcohol solvent consumption is 50L); Concentrate under reduced pressure at low temperature adds an amount of water and dissolves to there not being the alcohol flavor.Water-soluble liquid is crossed AB-8 type macroporous adsorbent resin; Elder generation is eluted to colourless with 3BV water and 3BV 15% ethanol (volume), remove macromole impurity such as desaccharide, discards; Reuse 5BV 80% ethanol (volume) eluting; Collect ethanol elution,, promptly get Radix Smilacis Chinae stem extract of the present invention (Rhizoma Smilacis Chinensis rhizome effective site) 191 grams the eluent drying under reduced pressure behind the recovery ethanol.
The preparation of embodiment 3 Radix Smilacis Chinae stem extracts
Get Rhizoma Smilacis Chinensis rhizome 5.0kg (available from Anguo medical material wholesale market, Henan, Hubei, the place of production), extract 24h, collect extracting solution with 100L60% ethanol (volume) percolation; Concentrate under reduced pressure at low temperature adds an amount of water and dissolves to there not being the alcohol flavor.Water-soluble liquid is crossed HPD-400 type macroporous adsorbent resin; Elder generation is eluted to colourless with 3BV water and 3BV 20% ethanol (volume), remove macromole impurity such as desaccharide, discards; Reuse 5BV 70% ethanol (volume) eluting; Collect ethanol elution,, promptly get Radix Smilacis Chinae stem extract of the present invention (Rhizoma Smilacis Chinensis rhizome effective site) 178 grams the eluent drying under reduced pressure behind the recovery ethanol.
The preparation of embodiment 4 Radix Smilacis Chinae stem extracts
Get Rhizoma Smilacis Chinensis rhizome 5.0kg (available from Anguo medical material wholesale market, Henan, Hubei, the place of production),, collect extracting solution with 70% methanol (volume) heating and refluxing extraction 3 times (1h, each alcohol solvent consumption is 50L for 2h, 1h); Concentrate under reduced pressure at low temperature adds an amount of water and dissolves to there not being the alcohol flavor.Water-soluble liquid is crossed NKA-9 type macroporous adsorbent resin; Elder generation is eluted to colourless with 3BV water and 3BV 20% ethanol (volume), remove macromole impurity such as desaccharide, discards; Reuse 5BV 70% ethanol (volume) eluting; Collect ethanol elution,, promptly get Radix Smilacis Chinae stem extract of the present invention (Rhizoma Smilacis Chinensis rhizome effective site) 180 grams the eluent drying under reduced pressure behind the recovery ethanol.
The analysis of embodiment 5 Radix Smilacis Chinae stem extract saponin contents
Adopt the content of total saponins constituents in the spectrophotometry Rhizoma Smilacis Chinensis extract:
The preparation of reference substance solution: precision takes by weighing dioscin (available from Nat'l Pharmaceutical & Biological Products Control Institute, article No. the is 111707-200501) reference substance of dry constant weight and uses dissolve with methanol, processes the solution that every 1ml contains the 0.4124mg dioscin, and is subsequent use.
The preparation of need testing solution: precision takes by weighing each 1g of extract that embodiment 1-4 makes, and places tool plug conical flask respectively, adds methanol 50ml, and accurate the title decided weight, and supersound extraction 40min weighs once more, adds methanol and supplies the weight that subtracts mistake, shakes up, and filters.Precision is drawn filtrating in right amount, crosses polyamide column (10g), uses 70% ethanol elution, revolves behind the collection 200ml eluent and steams to dried, and residue adds dissolve with methanol and is settled in the 10ml measuring bottle, shakes up subsequent use.
The drafting of standard curve: precision is measured dioscin reference substance solution (0.4124mg/ml) 0.5,0.4,0.3,0.2,0.1,0.05ml, volatilizes solvent, after showing by the method in following " algoscopy ", measures absorbance in the 407nm wavelength.With trap (y) is vertical coordinate, and concentration x (mg/ml) is an abscissa, and the drawing standard curve gets regression equation through match and is: y=16.938x-0.0154.
Algoscopy: it is an amount of to get need testing solution, after water-bath volatilizes solvent, adds perchloric acid 5ml, in 70 ℃ of water-bath colour developings, is blank with perchloric acid, according to spectrophotography (appendix VA of Chinese Pharmacopoeia version in 2005), measures trap in the 407nm wavelength.
The result who obtains is as shown in table 1.
Total saponin content among the table 1 embodiment 1-4
Embodiment 6 preparation of drug combination
Get the Radix Smilacis Chinae stem extract 10g of embodiment 1-4 respectively, sucrose 40g, mix homogeneously is granulated, and sieves, and drying promptly makes the medicine of Radix Smilacis Chinae stem extract granule dosage form; Maybe with the granule that makes through further tabletting, drying promptly makes the medicine of Radix Smilacis Chinae stem extract Tabules.
Embodiment 7 preparation of drug combination
Get the Radix Smilacis Chinae stem extract 10g of embodiment 1-4 respectively, micropowder silica gel 50g, mix homogeneously adopts the capsules preparation technique preparation, promptly gets the medicine of Radix Smilacis Chinae stem extract capsule formulation.
Embodiment 8 preparation of drug combination
Get the Radix Smilacis Chinae stem extract 10g of embodiment 1-4 respectively, add in fused polyethylene glycol 6000 of 10g and the 10g Macrogol 4000, adopt drop pill preparation technology, promptly make the medicine of Radix Smilacis Chinae stem extract drops.
Embodiment 9 preparation of drug combination
Get the Radix Smilacis Chinae stem extract 10g of embodiment 1-4 respectively, microcrystalline Cellulose 12g, sodium carboxymethyl cellulose 3g, micropowder silica gel 0.1g, mix homogeneously, tabletting promptly makes the medicine of Radix Smilacis Chinae stem extract tablet formulation.
Embodiment 10 preparation of drug combination
Get the Radix Smilacis Chinae stem extract 10g of embodiment 1-4 respectively, vegetable oil 25g, mixing is made capsule casing material with gelatin, is pressed into soft capsule, promptly makes the medicine of Radix Smilacis Chinae stem extract soft capsule dosage form.
Embodiment 11 preparation of drug combination
Get the Radix Smilacis Chinae stem extract 10g of embodiment 1-4 respectively, hydroxypropyl emthylcellulose 10g, sodium carboxymethyl cellulose 6g, lactose 4g, mix homogeneously is granulated, drying, tabletting promptly gets the medicine of Radix Smilacis Chinae stem extract slow releasing tablet dosage form.
Following examples will be used to prove the curative effect of Radix Smilacis Chinae stem extract of the present invention.
Embodiment 12
Present embodiment is used to explain the pharmacodynamic experiment of Radix Smilacis Chinae stem extract.
(1) the external antibacterial test of pesticide effectiveness
1. experiment material
1.1 bacterial strain:
Choose 4 kinds of urogenital infections common bacterias and fungus, staphylococcus aureus ATCC6538, escherichia coli ATCC 8739, gonococcus ATCC 49226, Candida albicans ATCC 90029.Above bacterial strain is available from the multiple auspicious bio tech ltd in Shanghai.
1.2 medicine:
Radix Smilacis Chinae stem extract (pressing the preparation of embodiment 1-4 method for preparing).
2. experimental technique and result
Get above-mentioned 4 kinds of strain cultures, the normal saline bacterium liquid of processing 100,000,000/ml is subsequent use, and wherein gonococcus is with containing the preparation of 10% calf serum normal saline.Every kind of bacterium liquid is inoculated dull and stereotyped 2 pieces of the pastille of corresponding variable concentrations respectively, and every plate 0.1ml puts 37 ℃ of incubators and cultivates the 24h observed results, inoculates not the pastille flat board simultaneously and does growth control.The dull and stereotyped bacterial growth of pastille is not good; The dull and stereotyped no bacterial growth person of pastille is for suppress bacterium fully; Its drug level is minimal inhibitory concentration (MIC); The result shows that the Rhizoma Smilacis Chinensis extract of the present invention's preparation has good inhibitory effect (seeing table 2) to staphylococcus aureus, escherichia coli and gonococcus.
The bacteriostasis of the prepared Rhizoma Smilacis Chinensis extract of table 2 embodiment 1-4
(2) the antiinflammatory test of pesticide effectiveness
1. experiment material
1.1 animal:
Kunming mouse, body weight 20-22g; The wistar rat, body weight 150-180g.
1.2 medicine:
Radix Smilacis Chinae stem extract (pressing the preparation of embodiment 1-4 method for preparing).
2. experimental technique and result
2.1 influence to the mouse ear edema
50 male mices are divided into 5 groups at random, 10 every group.Every day gastric infusion once, successive administration 3 days, matched group are given distilled water.Behind 30min after the last administration, drip Oleum Tiglii proinflammatory agent 0.05ml to every mice left side auricular concha, put to death animal behind the 2h; Cut left and right sides ear; Lay same position auricle with diameter 9mm card punch, weigh, with two auricle weight differences as the swollen degree of Mus ear; The result shows that the Rhizoma Smilacis Chinensis extract of the present invention's preparation has anti-inflammatory activity (seeing table 3) preferably.
The prepared Rhizoma Smilacis Chinensis extract of table 3 embodiment 1-4 is to the influence of mouse ear edema
Annotate: compare * P＜0.05 * * P＜0.01 with matched group
2.2 to the bullate influence of rat foot
50 male rats are divided into 5 groups at random, 10 every group.Every day gastric infusion once, successive administration 3 days, matched group are given distilled water.Behind 30min after the last administration; Cause inflammation at the right back sufficient pad subcutaneous injection 1.0% lrish moss mucilage 0.05ml of portion; 1h measures 0.5cm place diameter below every the right back ankle of rat joint before causing inflammation and after causing inflammation respectively; As the swelling degree, the result shows that the Rhizoma Smilacis Chinensis extract of the present invention's preparation has anti-inflammatory activity (seeing table 4) preferably with the difference that causes scorching front and back.
The prepared Rhizoma Smilacis Chinensis extract of table 4 embodiment 1-4 is to the bullate influence of rat foot
Annotate: compare * P＜0.05 * * P＜0.01 with matched group
Claims (10)
1. the method for preparing of Radix Smilacis Chinae stem extract is characterized in that, this method may further comprise the steps:
(1) with solvent extraction Rhizoma Smilacis Chinensis rhizome medical material;
(2) extracting solution is concentrated, be dissolved in water again;
(3) water-soluble liquid that step (2) is obtained passes through macroporous adsorbent resin, the methanol of water or 1～20 weight % or ethanol water drip washing;
(4) with methanol or the ethanol water eluting of 30～95 weight %, collect eluent,, remove and desolvate, be drying to obtain Rhizoma Smilacis Chinensis extract.
2. method for preparing as claimed in claim 1, wherein, the solvent in the said step (1) is selected from one or more in water, methanol, ethanol and the acetone.
3. method for preparing as claimed in claim 1, wherein, the method for distilling in the said step (1) is warm macerating, hot reflux and/or percolation.
4. method for preparing as claimed in claim 1, wherein, the macroporous adsorbent resin in the said step (3) is selected from one or more in nonpolar macroporous adsorption resin, low pole macroporous adsorbent resin, Semi-polarity macroporous adsorbent resin and the polar macroporous adsorption resin; Preferably, said macroporous adsorbent resin is selected from one or more in HPD-300 macroporous adsorbent resin, HPD-100 macroporous adsorbent resin, D101 macroporous adsorbent resin, X-5 macroporous adsorbent resin, H103 macroporous adsorbent resin, H107 macroporous adsorbent resin, AB-8 macroporous adsorbent resin, DA-201 macroporous adsorbent resin, HPD-400 macroporous adsorbent resin, ML-8 macroporous adsorbent resin, NKA-9 macroporous adsorbent resin, NKA-II macroporous adsorbent resin, S-8 macroporous adsorbent resin, HPD-500 macroporous adsorbent resin and the HPD-600 macroporous adsorbent resin.
5. like each described method for preparing among the claim 1-4, wherein, methanol in the said step (3) or ethanol water are methanol or the ethanol water of volume fraction 15%-20%.
6. like each described method for preparing among the claim 1-4, wherein, methanol in the said step (4) or ethanol water are methanol or the ethanol water of volume fraction 70%-80%.
7. a Radix Smilacis Chinae stem extract is characterized in that, it is made by the method that may further comprise the steps:
(1) with solvent extraction Rhizoma Smilacis Chinensis rhizome medical material;
(2) extracting solution is concentrated, be dissolved in water again;
(3) water-soluble liquid that step (2) is obtained passes through macroporous adsorbent resin, the methanol of water or 1～20 weight % or ethanol water drip washing;
(4) with methanol or the ethanol water eluting of 30～95 weight %, collect eluent, remove and desolvate, be drying to obtain Rhizoma Smilacis Chinensis extract.
8. the described Radix Smilacis Chinae stem extract of claim 7 is used for preventing, alleviating or treat the application of the medicine of inflammation in preparation.
9. application as claimed in claim 8, wherein, said inflammation is caused by staphylococcus aureus and escherichia coli; Preferably, said inflammation is a gynecological inflammation; More preferably, said gynecological inflammation is selected from acute pelvic inflammatory disease, chronic pelvic inflammatory disease, vaginitis, cervicitis, adnexitis, inflammatory masses of ovarian appendages.
10. purposes as claimed in claim 8; Wherein, said medicine is capsule, tablet, granule, drop pill, soft capsule, dispersible tablet, oral cavity disintegration tablet, slow releasing tablet, controlled release tablet, injection, transfusion or the injectable sterile powder that comprises the Radix Smilacis Chinae stem extract.
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| CN108434245A (en) * | 2018-06-20 | 2018-08-24 | 广东工业大学 | A kind of plant button son eggplant extract and its preparation method and application |
| KR102036339B1 (en) * | 2019-05-29 | 2019-10-25 | 동국대학교 경주캠퍼스 산학협력단 | Antiinflammatory, Antibacterial and Antifungal Composition containing Ulmus macrocarpa Hance, Leonurus sibiricus L. and Smilax china L. Root Extract and Pharmaceutical Composition for Preventing or Treating Vaginitis, and Composition for Female Cleansing Agent containing the same |
| KR102048525B1 (en) * | 2019-05-29 | 2019-11-26 | 동국대학교 경주캠퍼스 산학협력단 | Antiinflammatory, Antibacterial and Antifungal Composition containing Ulmus macrocarpa Hance and Smilax china L. Root Extract and Pharmaceutical Composition for Preventing or Treating Vaginitis, and Composition for Female Cleansing Agent containing the same |
| CN110776482A (en) * | 2018-07-30 | 2020-02-11 | 上海中医药大学 | Compound with activity of inhibiting macrophage from releasing NO, and preparation method and application thereof |
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| CN110776482B (en) * | 2018-07-30 | 2022-09-30 | 上海中医药大学 | Compound with activity of inhibiting macrophage from releasing NO, and preparation method and application thereof |
| KR102036339B1 (en) * | 2019-05-29 | 2019-10-25 | 동국대학교 경주캠퍼스 산학협력단 | Antiinflammatory, Antibacterial and Antifungal Composition containing Ulmus macrocarpa Hance, Leonurus sibiricus L. and Smilax china L. Root Extract and Pharmaceutical Composition for Preventing or Treating Vaginitis, and Composition for Female Cleansing Agent containing the same |
| KR102048525B1 (en) * | 2019-05-29 | 2019-11-26 | 동국대학교 경주캠퍼스 산학협력단 | Antiinflammatory, Antibacterial and Antifungal Composition containing Ulmus macrocarpa Hance and Smilax china L. Root Extract and Pharmaceutical Composition for Preventing or Treating Vaginitis, and Composition for Female Cleansing Agent containing the same |
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Application publication date: 20120919 |