CN102669183B - Biological pesticide combination used for controlling rice sheath blight and containing paenibacillus polymyxa, validamycin and trisiloxane polyoxyethylene ether - Google Patents
Biological pesticide combination used for controlling rice sheath blight and containing paenibacillus polymyxa, validamycin and trisiloxane polyoxyethylene ether Download PDFInfo
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- CN102669183B CN102669183B CN2012101125768A CN201210112576A CN102669183B CN 102669183 B CN102669183 B CN 102669183B CN 2012101125768 A CN2012101125768 A CN 2012101125768A CN 201210112576 A CN201210112576 A CN 201210112576A CN 102669183 B CN102669183 B CN 102669183B
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- paenibacillus polymyxa
- biological pesticide
- jinggangmeisu
- sheath blight
- rice sheath
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Abstract
The invention discloses a biological pesticide combination used for controlling rice sheath blight and containing paenibacillus polymyxa, validamycin and trisiloxane polyoxyethylene ether. The biological pesticide combination comprises paenibacillus polymyxa, validamycin and organosilicone. The paenibacillus polymyxa and the validamycin are made into mixture according to the weight ratio of 1:0.01-100. The trisiloxane polyoxyethylene ether is then added into the mixture according to the volume ratio of 95-97:3-5. The concentration of the trisiloxane polyoxyethylene ether is 95-97%. The auxiliary, trisiloxane polyoxyethylene ether, is added into biological pesticide to make the biological pesticide combination. Control effect of biological pesticide combination can be improved. Outdoor degradation rate of viable bacteria is slowed. Spreading of liquor of the biological pesticide combination is improved. Organic silicone oil is nontoxic, and accordingly the biological pesticide combination is environment-friendly.
Description
Technical field
The present invention relates to field of biological pesticide, is exactly the biological pesticide composite of Paenibacillus polymyxa, jinggangmeisu and the trisiloxanes APEO of preventing and treating rice sheath blight disease.
Background technology
Domestic rice sheath blight disease (Rhizoctonia solani) biopesticide of preventing and treating is mainly at present; Bacillus subtilis, bacillus polymyxa, waxy Bacillus and composite jinggangmycin thing, formulation is divided into two kinds, aqua and pulvis.Which kind of biopesticide is all to take spore and shaft-like thalline as main, and the pathogen of controlling rice sheath blight disease expands on rice plant, and this class viable bacteria body is prevented and treated the rice sheath blight disease effect and affected by outdoor temp, wet, intensity of illumination.Particularly the ubiquity of peasant's dispenser in recent years the amount of liquid medicine deficiency, and the rice sheath blight disease happening part is mainly in the paddy rice middle and lower part, because the not enough viable bacteria body of amount of liquid medicine is difficult to reach site of pathological change, Here it is biopesticide to the rice sheath blight disease preventive effect a little less than one of chemical pesticide principal element.
In order to address the above problem, we carried out first at home gram line spirit (agent of Paenibacillus polymyxa composite jinggangmycin) and added organic silicone oil (trisiloxanes APEO) this year, to the Rhizoctonia solani Kuhn field control effectiveness test, wherein national patent has been applied in the spirit of gram line, the patent No. 200810019537.7, patent name is: Paenibacillus polymyxa and the validamycin composition of preventing and treating rice sheath blight disease.
Summary of the invention
The purpose of this invention is to provide a kind of preventive effect that improves, slow down the viable bacteria body at outdoor degradation speed, improve liquid and expand on rice plant, organic silicone oil is nontoxic, the environment amenable agricultural chemicals of preventing and treating rice sheath blight disease.
Above-mentioned purpose realizes by following scheme:
Prevent and treat the Paenibacillus polymyxa of rice sheath blight disease, the biological pesticide composite of jinggangmeisu and trisiloxanes APEO, it is characterized in that: it is by Paenibacillus polymyxa, jinggangmeisu and trisiloxanes APEO form, wherein Paenibacillus polymyxa and jinggangmeisu form built agent according to weight ratio 1:0.01-100, the concentration 2000-4000CFU/ μ L of Paenibacillus polymyxa in built agent, the weight content of jinggangmeisu is 5-12%, add again the trisiloxanes APEO in described built agent, the volume ratio of built agent and trisiloxanes APEO is 95-97:3-5, described trisiloxanes APEO concentration is 95-97%.
The described biotinylated biomolecule composition pesticide of preventing and treating Paenibacillus polymyxa, jinggangmeisu and the trisiloxanes APEO of rice sheath blight disease is characterized in that: Paenibacillus polymyxa and jinggangmeisu form built agent according to weight ratio 1:50-80.
The described biotinylated biomolecule composition pesticide of preventing and treating Paenibacillus polymyxa, jinggangmeisu and the trisiloxanes APEO of rice sheath blight disease, it is characterized in that: described biological pesticide composite formulation is aqua.
The described biotinylated biomolecule composition pesticide of preventing and treating Paenibacillus polymyxa, jinggangmeisu and the trisiloxanes APEO of rice sheath blight disease, it is characterized in that: using method is: in fringe phase beginning, execute medicine for the first time, interval 11-13 days executes medicine for the second time, every 662-669 m
2Use biological pesticide composite 100ml.
Beneficial effect of the present invention is:
The present invention utilizes biopesticide to add organosilicon, be that trisiloxanes APEO auxiliary agent is as biological pesticide composite, can improve preventive effect, slow down the viable bacteria body at outdoor degradation speed, improve liquid expands on rice plant, organic silicone oil is nontoxic, environmentally friendly, thereby the organosilicon auxiliary agent is applied and had the space of developing on a large scale very much the biopesticide aspect.
Embodiment
Prevent and treat the biological pesticide composite gram line spirit of Paenibacillus polymyxa, jinggangmeisu and the trisiloxanes APEO of rice sheath blight disease. organosilicon, raw material: 200810019537.7 patents-Ke line spirit, wherein Paenibacillus polymyxa and jinggangmeisu form built agent according to weight ratio 1:0.01-100, the concentration 2000-4000CFU/ μ L of Paenibacillus polymyxa in built agent, the weight content of jinggangmeisu is 9-12%; Add the trisiloxanes APEO again in described built agent (spirit of gram line), built agent (spirit of gram line) is 95-97:3-5 with the volume ratio of trisiloxanes APEO, and described trisiloxanes APEO concentration is 95-97%.
Using method is: execute medicine for the first time in fringe phase beginning, medicine is for the second time executed at interval in 12 days, every 667 m
2Spray medicine 100 mL, be diluted to liquid 60L, uses the manual sprayer even spraying.
Carry out comparative trial with other agricultural chemicals:
1.1 test material
The development of reagent agent (1) gram line spirit Institute of Botany Protection
(2) gram line spirit. the development of organosilicon (being the present invention) Institute of Botany Protection
(3) 20% jinggangmycin A F Zhejiang Tonglu Huifeng Bio-Chemical Co., Ltd.
(4) 40% wax bud bacterium WP Jiangsu Province, well ridge Zhongnan Chemical Industry Co., Ltd., Liyang.
Field control effectiveness test is located at Meiling Hill village, Lu'an City Shucheng County Shu Cha town on August 18th, 2011 to carry out in experimental field.For the examination conventional rice variety, be new two excellent No. 6, twice totally dispenser.
1.2 test method
Test is established 4 processing, 1: gram line spirit 100mL/667m
2, 2: the spirit of gram line. and organosilicon (being the present invention) 100 mL/ 667m
2, 3:20% jinggangmycin A F62.5g/667m
2, 4:40% well ridge wax bud bacterium WP60g/667m
2, 5: the clear water contrast.Every processing is established and repeated ，Mei community area 3 times is 40m
2, the community randomized arrangement, the cultivation condition of all treatment regions is consistent.Fringe phase beginning is executed medicine for the first time, and medicine is for the second time executed at interval in 12 days.Every 667 m
2Spraying liquid 60L, by WS-16P type manual sprayer even spraying.
Investigation method and drug effect are calculated by " bactericidal agent for preventing and treating rice sheath blight disease test of pesticide effectiveness criterion " and are undertaken, and adopt 5 samplings of diagonal, and every fixing investigation is connected 5 clumps, totally 25 clumps, divide 9 grades of records, calculate disease and refer to and control efficiency, and carry out significance of difference test by the DMRT method.
2, result and discussion
Spraying time is August 10 for the first time, and the medicine time is August 25 for the second time.Investigation state of an illness radix before dispenser, 10 days investigation preventive effects after dispenser for the second time.Result is as follows;
Disease index before and after table 1 control
| Chemicals treatment | Before medicine, disease refers to | After medicine, disease refers to | Preventive effect (%) | Variance analysis |
| The spirit of gram line | 10.46 | 12.56 | 65.67 | a? A |
| The spirit of gram line. organosilicon | 11.94 | 11.02 | 79.51 | a? A |
| 20% jinggangmycin A F | 11.83 | 14.76 | 63.76 | a? A |
| 40% well ridge wax bud bacterium WP | 11.69 | 13.02 | 64.57 | a? A |
| Blank | 11.75 | 42.90 | — | — |
By table 1, can be drawn, the agent of investigation result gram line spirit organic silicon compound will be apparently higher than the control efficiency of other biological agricultural chemicals, from result for the second time, can find out, the present invention reaches the control efficiency 64.57% of 79.51%, 40% well ridge wax bud bacterium to the control efficiency of rice sheath blight disease.The preventive effect 65.67% of gram line spirit to rice sheath blight disease.
By the field control effectiveness test result, show; Biopesticide adds organic silicon additive can improve preventive effect, slows down the viable bacteria body at outdoor degradation speed, improves liquid and expands on rice plant, and organic silicone oil is nontoxic, environmentally friendly, thereby the organosilicon auxiliary agent is applied and had the space of developing on a large scale very much the biopesticide aspect.
Claims (4)
1. prevent and treat the Paenibacillus polymyxa of rice sheath blight disease, the biological pesticide composite of jinggangmeisu and trisiloxanes APEO, it is characterized in that: it is by Paenibacillus polymyxa, jinggangmeisu and trisiloxanes APEO form, wherein Paenibacillus polymyxa and jinggangmeisu form built agent according to weight ratio 1:0.01-100, the concentration 2000-4000CFU/ μ L of Paenibacillus polymyxa in built agent, the weight content of jinggangmeisu is 5-12%, add again the trisiloxanes APEO in described built agent, the volume ratio of built agent and trisiloxanes APEO is 95-97:3-5, described trisiloxanes APEO concentration is 95-97%.
2. the biotinylated biomolecule composition pesticide of preventing and treating Paenibacillus polymyxa, jinggangmeisu and the trisiloxanes APEO of rice sheath blight disease according to claim 1 is characterized in that: Paenibacillus polymyxa and jinggangmeisu form built agent according to weight ratio 1:50-80.
3. the biotinylated biomolecule composition pesticide of preventing and treating Paenibacillus polymyxa, jinggangmeisu and the trisiloxanes APEO of rice sheath blight disease according to claim 1, it is characterized in that: described biological pesticide composite formulation is aqua.
4. the biotinylated biomolecule composition pesticide of preventing and treating Paenibacillus polymyxa, jinggangmeisu and the trisiloxanes APEO of rice sheath blight disease according to claim 1, it is characterized in that: using method is: in fringe phase beginning, execute medicine for the first time, interval 11-13 days executes medicine for the second time, every 662-669 m
2Use biological pesticide composite 100ml.
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| CN2012101125768A CN102669183B (en) | 2012-04-17 | 2012-04-17 | Biological pesticide combination used for controlling rice sheath blight and containing paenibacillus polymyxa, validamycin and trisiloxane polyoxyethylene ether |
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| CN2012101125768A CN102669183B (en) | 2012-04-17 | 2012-04-17 | Biological pesticide combination used for controlling rice sheath blight and containing paenibacillus polymyxa, validamycin and trisiloxane polyoxyethylene ether |
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| CN102669183B true CN102669183B (en) | 2013-12-04 |
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| CN103329946B (en) * | 2013-06-21 | 2015-06-17 | 苏州市阳澄湖现代农业发展有限公司 | Oil suspension pesticide composition for preventing and treating rice sheath blight disease and preparation method thereof |
| CN103329947B (en) * | 2013-06-21 | 2015-08-19 | 苏州市阳澄湖现代农业发展有限公司 | Biological pesticide composite of a kind of water prevention rice sheath blight and preparation method thereof |
| CN103875714A (en) * | 2014-03-07 | 2014-06-25 | 安徽省农业科学院植物保护与农产品质量安全研究所 | Bacillus thuringiensis.spinosyns.trisiloxane polyoxyethylene ether composition and use of bacillus thuringiensis.spinosyns.trisiloxane polyoxyethylene ether composition for preventing and controlling rice boring insects |
| CN104336091B (en) * | 2014-09-28 | 2016-08-10 | 句容市农业技术推广中心 | A kind of rice sheath blight disease controlling medicament |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101914600A (en) * | 2010-08-27 | 2010-12-15 | 上海交通大学 | Validamycin optimal fermentation method based on hydrogen peroxide |
| CN101921244A (en) * | 2010-09-02 | 2010-12-22 | 南开大学 | Derivative of 4-methyl-1,2,3-thiadiazole containing 4-(1,1,2,2-tetrafluoroethoxy)-3,5-dichlorobenzene amino and synthesis method and application thereof |
| CN101942403A (en) * | 2010-06-12 | 2011-01-12 | 浙江大学 | Bacillus pumilus as well as culture method and application thereof |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101942403A (en) * | 2010-06-12 | 2011-01-12 | 浙江大学 | Bacillus pumilus as well as culture method and application thereof |
| CN101914600A (en) * | 2010-08-27 | 2010-12-15 | 上海交通大学 | Validamycin optimal fermentation method based on hydrogen peroxide |
| CN101921244A (en) * | 2010-09-02 | 2010-12-22 | 南开大学 | Derivative of 4-methyl-1,2,3-thiadiazole containing 4-(1,1,2,2-tetrafluoroethoxy)-3,5-dichlorobenzene amino and synthesis method and application thereof |
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