CN102669183A - Biological pesticide combination used for controlling rice sheath blight and containing paenibacillus polymyxa, validamycin and trisiloxane polyoxyethylene ether - Google Patents
Biological pesticide combination used for controlling rice sheath blight and containing paenibacillus polymyxa, validamycin and trisiloxane polyoxyethylene ether Download PDFInfo
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- CN102669183A CN102669183A CN2012101125768A CN201210112576A CN102669183A CN 102669183 A CN102669183 A CN 102669183A CN 2012101125768 A CN2012101125768 A CN 2012101125768A CN 201210112576 A CN201210112576 A CN 201210112576A CN 102669183 A CN102669183 A CN 102669183A
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- paenibacillus polymyxa
- biological pesticide
- jinggangmeisu
- sheath blight
- rice sheath
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Abstract
The invention discloses a biological pesticide combination used for controlling rice sheath blight and containing paenibacillus polymyxa, validamycin and trisiloxane polyoxyethylene ether. The biological pesticide combination comprises paenibacillus polymyxa, validamycin and organosilicone. The paenibacillus polymyxa and the validamycin are made into mixture according to the weight ratio of 1:0.01-100. The trisiloxane polyoxyethylene ether is then added into the mixture according to the volume ratio of 95-97:3-5. The concentration of the trisiloxane polyoxyethylene ether is 95-97%. The auxiliary, trisiloxane polyoxyethylene ether, is added into biological pesticide to make the biological pesticide combination. Control effect of biological pesticide combination can be improved. Outdoor degradation rate of viable bacteria is slowed. Spreading of liquor of the biological pesticide combination is improved. Organic silicone oil is nontoxic, and accordingly the biological pesticide combination is environment-friendly.
Description
Technical field
The present invention relates to field of biological pesticide, exactly is the biological pesticide composite of Paenibacillus polymyxa, jinggangmeisu and the trisiloxanes APEO of preventing and treating rice sheath blight disease.
Background technology
Domestic rice sheath blight disease (Rhizoctonia solani) biopesticide of preventing and treating mainly is at present; Bacillus subtilis, bacillus polymyxa, waxy Bacillus and composite jinggangmycin thing, formulation are divided into two kinds in aqua and pulvis.Which kind of biopesticide all is to be main with spore and shaft-like thalline, and the pathogen of control rice sheath blight disease expands on rice plant, and this type viable bacteria body is prevented and treated the rice sheath blight disease effect and influenced by outdoor temperature, wet, intensity of illumination.Particularly the ubiquity of peasant's dispenser in recent years the amount of liquid medicine deficiency; And the rice sheath blight disease happening part is mainly in the paddy rice middle and lower part; The viable bacteria body is difficult to reach site of pathological change because amount of liquid medicine is not enough, Here it is biopesticide to the rice sheath blight disease preventive effect a little less than one of chemical pesticide principal element.
In order to address the above problem; We carried out gram line spirit (agent of Paenibacillus polymyxa composite jinggangmycin) at home first and added organic silicone oil (trisiloxanes APEO) this year; To the Rhizoctonia solani Kuhn field control effectiveness test; Wherein restrain the line spirit and applied for national patent, the patent No. 200810019537.7, patent name is: Paenibacillus polymyxa and the jinggangmeisu composition of preventing and treating rice sheath blight disease.
Summary of the invention
The purpose of this invention is to provide a kind of preventive effect that improves, slow down the viable bacteria body at outdoor degradation speed, improve soup and on rice plant, expand, organic silicone oil is nontoxic, the environment amenable agricultural chemicals of preventing and treating rice sheath blight disease.
Above-mentioned purpose realizes through following scheme:
Prevent and treat the biological pesticide composite of Paenibacillus polymyxa, jinggangmeisu and the trisiloxanes APEO of rice sheath blight disease; It is characterized in that: it is made up of Paenibacillus polymyxa, jinggangmeisu and trisiloxanes APEO; Wherein Paenibacillus polymyxa and jinggangmeisu are formed composite dose according to weight ratio 1:0.01-100; The concentration 2000-4000CFU/ μ L of Paenibacillus polymyxa in composite dose; The weight content of jinggangmeisu is 5-12%; In described composite dose, add the trisiloxanes APEO again, the composite dose of volume ratio with the trisiloxanes APEO is 95-97:3-5, and described trisiloxanes APEO concentration is 95-97%.
The described biotinylated biomolecule composition pesticide of preventing and treating Paenibacillus polymyxa, jinggangmeisu and the trisiloxanes APEO of rice sheath blight disease is characterized in that: Paenibacillus polymyxa and jinggangmeisu are formed composite dose according to weight ratio 1:50-80.
The described biotinylated biomolecule composition pesticide of preventing and treating Paenibacillus polymyxa, jinggangmeisu and the trisiloxanes APEO of rice sheath blight disease, it is characterized in that: described biological pesticide composite formulation is an aqua.
The described biotinylated biomolecule composition pesticide of preventing and treating Paenibacillus polymyxa, jinggangmeisu and the trisiloxanes APEO of rice sheath blight disease; It is characterized in that: method for using is: execute medicine for the first time in fringe phase beginning; Executed medicine for the second time at interval in 11-13 days, every 662-669 m
2Use biological pesticide composite 100ml.
Beneficial effect of the present invention is:
The present invention utilizes biopesticide to add organosilicon; Be trisiloxanes APEO auxiliary agent as biological pesticide composite, can improve preventive effect, slow down the viable bacteria body at outdoor degradation speed; Improving soup expands on rice plant; Organic silicone oil is nontoxic, and is environmentally friendly, thereby the organosilicon auxiliary agent is used the biopesticide aspect and had the space of developing on a large scale very much.
Embodiment
Prevent and treat the biological pesticide composite gram line spirit of Paenibacillus polymyxa, jinggangmeisu and the trisiloxanes APEO of rice sheath blight disease. organosilicon; Raw material: 200810019537.7 patents-Ke line spirit; Wherein Paenibacillus polymyxa and jinggangmeisu are formed composite dose according to weight ratio 1:0.01-100; The concentration 2000-4000CFU/ μ L of Paenibacillus polymyxa in composite dose, the weight content of jinggangmeisu is 9-12%; In described composite dose (spirit of gram line), add the trisiloxanes APEO again, composite dose (spirit of gram line) is 95-97:3-5 with the volume ratio of trisiloxanes APEO, and described trisiloxanes APEO concentration is 95-97%.
Method for using is: execute medicine for the first time in fringe phase beginning, executed medicine for the second time at interval in 12 days, per 667 m
2Spray medicine 100 mL are diluted to soup 60L, use the manual sprayer even spraying.
Compare test with other agricultural chemicals:
1.1 test material
Supply the development of reagent agent (1) gram line spirit Institute of Botany Protection
(2) gram line spirit. the development of organosilicon (being the present invention) Institute of Botany Protection
(3) 20% jinggangmycin A F Zhejiang Tonglu Huifeng Bio-Chemical Co., Ltd.
Wax bud bacterium WP Jiangsu Province, (4) 40% well ridge Zhongnan Chemical Industry Co., Ltd., Liyang.
Field control effectiveness test is located at the easypro Cha Zhen Meiling Hill village of Lu'an City Shucheng County on August 18th, 2011 to carry out in the experimental field.Supply the examination conventional rice variety is new two excellent No. 6, twice totally dispenser.
1.2 test method
Test is established 4 processing, 1: gram line spirit 100mL/667m
2, 2: the spirit of gram line. and organosilicon (being the present invention) 100 mL/ 667m
2, 3:20% jinggangmycin A F62.5g/667m
2, 4:40% well ridge wax bud bacterium WP60g/667m
2, 5: the clear water contrast.3 repetitions are established in every processing, and every sub-district area is 40m
2, the sub-district randomized arrangement, the cultivation condition of all treatment regions is consistent.Fringe phase beginning is executed medicine for the first time, executes medicine for the second time at interval in 12 days.Per 667 m
2Spraying liquid 60L is with WS-16P type manual sprayer even spraying.
Investigation method and drug effect are calculated by " bactericidal agent for preventing and treating rice sheath blight disease test of pesticide effectiveness criterion " and are undertaken, and adopt 5 samplings of diagonal, and every fixing investigation links to each other 5 clumps; Totally 25 clumps; Divide 9 grades of records, calculate disease and refer to and control efficiency, and carry out significance of difference test with the DMRT method.
2, result and discussion
Spraying time is August 10 for the first time, and the medicine time is August 25 for the second time.Investigation state of an illness radix before the dispenser, 10 days investigation preventive effects after the dispenser for the second time.The result is following;
Disease index before and after table 1 control
| Chemicals treatment | Disease refers to before the medicine | Disease refers to behind the medicine | Preventive effect (%) | Variance analysis |
| The spirit of gram line | 10.46 | 12.56 | 65.67 | a? A |
| The spirit of gram line. organosilicon | 11.94 | 11.02 | 79.51 | a? A |
| 20% jinggangmycin A F | 11.83 | 14.76 | 63.76 | a? A |
| 40% well ridge wax bud bacterium WP | 11.69 | 13.02 | 64.57 | a? A |
| Blank | 11.75 | 42.90 | — | — |
Can draw by table 1; The agent of investigation result gram line spirit organic silicon compound will be apparently higher than the control efficiency of other biological agricultural chemicals; The result can find out from the second time, and the present invention reaches the control efficiency 64.57% of 79.51%, 40% well ridge wax bud bacterium to the control efficiency of rice sheath blight disease.The spirit of gram line is to the preventive effect 65.67% of rice sheath blight disease.
The result shows through field control effectiveness test; Biopesticide adds organic silicon additive can improve preventive effect, slows down the viable bacteria body at outdoor degradation speed, improves soup and on rice plant, expands, and organic silicone oil is nontoxic, and is environmentally friendly, thereby the organosilicon auxiliary agent is used the biopesticide aspect and had the space of developing on a large scale very much.
Claims (4)
1. prevent and treat the biological pesticide composite of Paenibacillus polymyxa, jinggangmeisu and the trisiloxanes APEO of rice sheath blight disease; It is characterized in that: it is made up of Paenibacillus polymyxa, jinggangmeisu and trisiloxanes APEO; Wherein Paenibacillus polymyxa and jinggangmeisu are formed composite dose according to weight ratio 1:0.01-100; The concentration 2000-4000CFU/ μ L of Paenibacillus polymyxa in composite dose; The weight content of jinggangmeisu is 5-12%; In described composite dose, add the trisiloxanes APEO again, the composite dose of volume ratio with the trisiloxanes APEO is 95-97:3-5, and described trisiloxanes APEO concentration is 95-97%.
2. the biotinylated biomolecule composition pesticide of preventing and treating Paenibacillus polymyxa, jinggangmeisu and the trisiloxanes APEO of rice sheath blight disease according to claim 1 is characterized in that: Paenibacillus polymyxa and jinggangmeisu are formed composite dose according to weight ratio 1:50-80.
3. the biotinylated biomolecule composition pesticide of preventing and treating Paenibacillus polymyxa, jinggangmeisu and the trisiloxanes APEO of rice sheath blight disease according to claim 1, it is characterized in that: described biological pesticide composite formulation is an aqua.
4. the biotinylated biomolecule composition pesticide of preventing and treating Paenibacillus polymyxa, jinggangmeisu and the trisiloxanes APEO of rice sheath blight disease according to claim 1; It is characterized in that: method for using is: execute medicine for the first time in fringe phase beginning; Executed medicine for the second time at interval in 11-13 days, every 662-669 m
2Use biological pesticide composite 100ml.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101125768A CN102669183B (en) | 2012-04-17 | 2012-04-17 | Biological pesticide combination used for controlling rice sheath blight and containing paenibacillus polymyxa, validamycin and trisiloxane polyoxyethylene ether |
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| CN2012101125768A CN102669183B (en) | 2012-04-17 | 2012-04-17 | Biological pesticide combination used for controlling rice sheath blight and containing paenibacillus polymyxa, validamycin and trisiloxane polyoxyethylene ether |
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| CN102669183A true CN102669183A (en) | 2012-09-19 |
| CN102669183B CN102669183B (en) | 2013-12-04 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103329946A (en) * | 2013-06-21 | 2013-10-02 | 苏州市阳澄湖现代农业发展有限公司 | Oil suspension pesticide composition for preventing and treating rice sheath blight disease and preparation method thereof |
| CN103329947A (en) * | 2013-06-21 | 2013-10-02 | 苏州市阳澄湖现代农业发展有限公司 | Biological pesticide composition for preventing and treating rice sheath blight disease and preparation method thereof |
| CN103875714A (en) * | 2014-03-07 | 2014-06-25 | 安徽省农业科学院植物保护与农产品质量安全研究所 | Bacillus thuringiensis.spinosyns.trisiloxane polyoxyethylene ether composition and use of bacillus thuringiensis.spinosyns.trisiloxane polyoxyethylene ether composition for preventing and controlling rice boring insects |
| CN104336091A (en) * | 2014-09-28 | 2015-02-11 | 句容市农业技术推广中心 | Rice sheath blight disease prevention and control agent |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101914600A (en) * | 2010-08-27 | 2010-12-15 | 上海交通大学 | Validamycin optimal fermentation method based on hydrogen peroxide |
| CN101921244A (en) * | 2010-09-02 | 2010-12-22 | 南开大学 | Derivative of 4-methyl-1,2,3-thiadiazole containing 4-(1,1,2,2-tetrafluoroethoxy)-3,5-dichlorobenzene amino and synthesis method and application thereof |
| CN101942403A (en) * | 2010-06-12 | 2011-01-12 | 浙江大学 | Bacillus pumilus as well as culture method and application thereof |
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2012
- 2012-04-17 CN CN2012101125768A patent/CN102669183B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101942403A (en) * | 2010-06-12 | 2011-01-12 | 浙江大学 | Bacillus pumilus as well as culture method and application thereof |
| CN101914600A (en) * | 2010-08-27 | 2010-12-15 | 上海交通大学 | Validamycin optimal fermentation method based on hydrogen peroxide |
| CN101921244A (en) * | 2010-09-02 | 2010-12-22 | 南开大学 | Derivative of 4-methyl-1,2,3-thiadiazole containing 4-(1,1,2,2-tetrafluoroethoxy)-3,5-dichlorobenzene amino and synthesis method and application thereof |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103329946A (en) * | 2013-06-21 | 2013-10-02 | 苏州市阳澄湖现代农业发展有限公司 | Oil suspension pesticide composition for preventing and treating rice sheath blight disease and preparation method thereof |
| CN103329947A (en) * | 2013-06-21 | 2013-10-02 | 苏州市阳澄湖现代农业发展有限公司 | Biological pesticide composition for preventing and treating rice sheath blight disease and preparation method thereof |
| CN103329946B (en) * | 2013-06-21 | 2015-06-17 | 苏州市阳澄湖现代农业发展有限公司 | Oil suspension pesticide composition for preventing and treating rice sheath blight disease and preparation method thereof |
| CN103329947B (en) * | 2013-06-21 | 2015-08-19 | 苏州市阳澄湖现代农业发展有限公司 | Biological pesticide composite of a kind of water prevention rice sheath blight and preparation method thereof |
| CN103875714A (en) * | 2014-03-07 | 2014-06-25 | 安徽省农业科学院植物保护与农产品质量安全研究所 | Bacillus thuringiensis.spinosyns.trisiloxane polyoxyethylene ether composition and use of bacillus thuringiensis.spinosyns.trisiloxane polyoxyethylene ether composition for preventing and controlling rice boring insects |
| CN104336091A (en) * | 2014-09-28 | 2015-02-11 | 句容市农业技术推广中心 | Rice sheath blight disease prevention and control agent |
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